Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also... present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains.
Кључне речи:
16S rDNA / lactobacilli / oral microflora / probiotic / repetitive sequence-based polymerase chain reactionИзвор:
Oral Microbiology & Immunology, 2007, 22, 2, 111-117Издавач:
- Wiley, Hoboken
DOI: 10.1111/j.1399-302X.2007.00331.x
ISSN: 0902-0055
PubMed: 17311634
WoS: 000244279800006
Scopus: 2-s2.0-33847043017
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Strahinić, I. AU - Busarcević, M. AU - Pavlica, Dušan AU - Milašin, Jelena AU - Golić, N. AU - Topisirović, L. PY - 2007 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1338 AB - Introduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains. PB - Wiley, Hoboken T2 - Oral Microbiology & Immunology T1 - Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates VL - 22 IS - 2 SP - 111 EP - 117 DO - 10.1111/j.1399-302X.2007.00331.x ER -
@article{ author = "Strahinić, I. and Busarcević, M. and Pavlica, Dušan and Milašin, Jelena and Golić, N. and Topisirović, L.", year = "2007", abstract = "Introduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains.", publisher = "Wiley, Hoboken", journal = "Oral Microbiology & Immunology", title = "Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates", volume = "22", number = "2", pages = "111-117", doi = "10.1111/j.1399-302X.2007.00331.x" }
Strahinić, I., Busarcević, M., Pavlica, D., Milašin, J., Golić, N.,& Topisirović, L.. (2007). Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. in Oral Microbiology & Immunology Wiley, Hoboken., 22(2), 111-117. https://doi.org/10.1111/j.1399-302X.2007.00331.x
Strahinić I, Busarcević M, Pavlica D, Milašin J, Golić N, Topisirović L. Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. in Oral Microbiology & Immunology. 2007;22(2):111-117. doi:10.1111/j.1399-302X.2007.00331.x .
Strahinić, I., Busarcević, M., Pavlica, Dušan, Milašin, Jelena, Golić, N., Topisirović, L., "Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates" in Oral Microbiology & Immunology, 22, no. 2 (2007):111-117, https://doi.org/10.1111/j.1399-302X.2007.00331.x . .