Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus
Apstrakt
Purpose: The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC. Methods: Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain. Results: Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in Has compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intens...e in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression. Conclusion: Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy.
Ključne reči:
Bcl-2 / oral lichen planus / premalignant lesion / squamous cell carcinomaIzvor:
Journal of BUON, 2010, 15, 2, 362-368Izdavač:
- Balkan Union of Oncology (B.U.ON.)
Finansiranje / projekti:
- The Laboratory of Oral Pathology, "Pasteur" Hospital, Nice, France
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Hadži-Mihailović, Miloš AU - Raybaud, Helene AU - Montei, R. AU - Čakić, Saša AU - Đurić, M. AU - Janković, Ljiljana PY - 2010 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1591 AB - Purpose: The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC. Methods: Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain. Results: Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in Has compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intense in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression. Conclusion: Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of BUON T1 - Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus VL - 15 IS - 2 SP - 362 EP - 368 UR - https://hdl.handle.net/21.15107/rcub_smile_1591 ER -
@article{ author = "Hadži-Mihailović, Miloš and Raybaud, Helene and Montei, R. and Čakić, Saša and Đurić, M. and Janković, Ljiljana", year = "2010", abstract = "Purpose: The aim of this study was to investigate the malignant potential of oral lichen planus (OLP) on the basis of the expression of the Bcl-2 marker in healthy individuals (H), patients with OLP and patients with squamous cell carcinoma (SCC), and to establish potential interdependence between expression of Bcl-2 and the different clinical and histopathological parameters in H, OLP, and SCC. Methods: Immunohistochemistry for Bcl-2 was carried out using an avidin-biotin peroxidase complex method. Tissue sections were treated with mouse monoclonal antibody against Bcl-2 (124, DAKO A/S, Denmark; dilution 1/100). Immunohistochemical parameters measured included total tissue area, total stained area and intensity of stain. Results: Keratinocytes were not Bcl-2 positive in H and were sparsely positive in OLP. The number of lymphocytes stained with Bcl-2 was significantly lower in Has compared to patients with SCC and OLP. Bcl-2 staining was weak to moderate in OLP, and moderate to intense in SCC. Bcl-2-positive lymphocytes were more expressed in older OLP patients (>55 years), and in OLP specimens with orthokeratinized epithelium, less developed acanthosis and highest grade of lymphocyte expression. Conclusion: Bcl-2 may not serve as a prognostic biomarker in oral cancer development from OLP, but it could help in selecting patients with higher need of follow up to prevent malignancy.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of BUON", title = "Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus", volume = "15", number = "2", pages = "362-368", url = "https://hdl.handle.net/21.15107/rcub_smile_1591" }
Hadži-Mihailović, M., Raybaud, H., Montei, R., Čakić, S., Đurić, M.,& Janković, L.. (2010). Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus. in Journal of BUON Balkan Union of Oncology (B.U.ON.)., 15(2), 362-368. https://hdl.handle.net/21.15107/rcub_smile_1591
Hadži-Mihailović M, Raybaud H, Montei R, Čakić S, Đurić M, Janković L. Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus. in Journal of BUON. 2010;15(2):362-368. https://hdl.handle.net/21.15107/rcub_smile_1591 .
Hadži-Mihailović, Miloš, Raybaud, Helene, Montei, R., Čakić, Saša, Đurić, M., Janković, Ljiljana, "Bcl-2 expression and its possible influence on malignant transformation of oral lichen planus" in Journal of BUON, 15, no. 2 (2010):362-368, https://hdl.handle.net/21.15107/rcub_smile_1591 .