Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits
Samo za registrovane korisnike
2015
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest ...that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.
Ključne reči:
diabetes / parotid secretion / inducible nitric oxide synthase / oxidative stressIzvor:
Oral Diseases, 2015, 21, 5, 634-640Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Kontrola bola i molekularni mehanizmi kao faktori regenerativne terapije u stomatologiji kod zdravih i pacijenata sa dijabetes melitusom (RS-MESTD-Basic Research (BR or ON)-175021)
DOI: 10.1111/odi.12327
ISSN: 1354-523X
PubMed: 25703905
WoS: 000355741700012
Scopus: 2-s2.0-84930276185
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Roganović, Jelena AU - Đukić, Ljiljana AU - Kršljak, Elena AU - Tanić, Nasta AU - Stojić, Dragica PY - 2015 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1963 AB - ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response. PB - Wiley, Hoboken T2 - Oral Diseases T1 - Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits VL - 21 IS - 5 SP - 634 EP - 640 DO - 10.1111/odi.12327 ER -
@article{ author = "Roganović, Jelena and Đukić, Ljiljana and Kršljak, Elena and Tanić, Nasta and Stojić, Dragica", year = "2015", abstract = "ObjectivesThe influence of experimental diabetes (alloxan, 100mgkg(-1)) was studied on rabbit parotid gland function. Material and MethodsCarbachol-induced parotid secretion in vivo, and in vitro quantification of inducible nitric oxide synthase (iNOS) mRNA expression, by real-time RT-PCR, and activity of superoxide dismutase (SOD) and total antioxidant capacity (TAC) in commercial colorimetric assays were measured in parotid glands of non-diabetic and diabetic rabbits. ResultsCarbachol-induced dose-dependent increase in parotid secretion significantly reduced in diabetic rabbits. Functional studies in the presence of muscarinic receptor and nitric oxide synthase (NOS) antagonists revealed that in M-3 receptor-mediated carbachol secretion, nitric oxide, deriving mainly from neuronal NOS (nNOS) in control, and iNOS in diabetic rabbits, was involved. Also, upregulation of iNOS mRNA expression and enhanced SOD activity and TAC were detected in diabetic glands. ConclusionsOur data suggest that decreased M-3 receptor-mediated parotid secretion in diabetic rabbits appears to be due to alterations in NO signaling, mainly due to iNOS induction, accompanied by elevated antioxidant response.", publisher = "Wiley, Hoboken", journal = "Oral Diseases", title = "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits", volume = "21", number = "5", pages = "634-640", doi = "10.1111/odi.12327" }
Roganović, J., Đukić, L., Kršljak, E., Tanić, N.,& Stojić, D.. (2015). Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases Wiley, Hoboken., 21(5), 634-640. https://doi.org/10.1111/odi.12327
Roganović J, Đukić L, Kršljak E, Tanić N, Stojić D. Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits. in Oral Diseases. 2015;21(5):634-640. doi:10.1111/odi.12327 .
Roganović, Jelena, Đukić, Ljiljana, Kršljak, Elena, Tanić, Nasta, Stojić, Dragica, "Reduced muscarinic parotid secretion is underlain by impaired NO signaling in diabetic rabbits" in Oral Diseases, 21, no. 5 (2015):634-640, https://doi.org/10.1111/odi.12327 . .