Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts
Samo za registrovane korisnike
2016
Autori
Velicković, MilenaPejnović, Nada
Petrović, Renata
Mitrović, Slobodanka
Jeftić, Ilija
Kanjevac, Tatjana
Lukić, Aleksandra
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of ...IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.
Ključne reči:
inflammation / interleukin-33 / periapical granuloma / radicular cyst / ST2Izvor:
Journal of Oral Pathology & Medicine, 2016, 45, 1, 70-76Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Uticaj IL-33/ST2 signalnog puta i galektina-3 u patogenezi eksperimentalnih periapikalnih promena (RS-MESTD-Basic Research (BR or ON)-175071)
DOI: 10.1111/jop.12312
ISSN: 0904-2512
PubMed: 25677987
WoS: 000369990100012
Scopus: 2-s2.0-84955747679
Kolekcije
Institucija/grupa
Stomatološki fakultetTY - JOUR AU - Velicković, Milena AU - Pejnović, Nada AU - Petrović, Renata AU - Mitrović, Slobodanka AU - Jeftić, Ilija AU - Kanjevac, Tatjana AU - Lukić, Aleksandra PY - 2016 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2135 AB - BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis. PB - Wiley, Hoboken T2 - Journal of Oral Pathology & Medicine T1 - Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts VL - 45 IS - 1 SP - 70 EP - 76 DO - 10.1111/jop.12312 ER -
@article{ author = "Velicković, Milena and Pejnović, Nada and Petrović, Renata and Mitrović, Slobodanka and Jeftić, Ilija and Kanjevac, Tatjana and Lukić, Aleksandra", year = "2016", abstract = "BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P lt 0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.", publisher = "Wiley, Hoboken", journal = "Journal of Oral Pathology & Medicine", title = "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts", volume = "45", number = "1", pages = "70-76", doi = "10.1111/jop.12312" }
Velicković, M., Pejnović, N., Petrović, R., Mitrović, S., Jeftić, I., Kanjevac, T.,& Lukić, A.. (2016). Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine Wiley, Hoboken., 45(1), 70-76. https://doi.org/10.1111/jop.12312
Velicković M, Pejnović N, Petrović R, Mitrović S, Jeftić I, Kanjevac T, Lukić A. Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine. 2016;45(1):70-76. doi:10.1111/jop.12312 .
Velicković, Milena, Pejnović, Nada, Petrović, Renata, Mitrović, Slobodanka, Jeftić, Ilija, Kanjevac, Tatjana, Lukić, Aleksandra, "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts" in Journal of Oral Pathology & Medicine, 45, no. 1 (2016):70-76, https://doi.org/10.1111/jop.12312 . .