Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions
2017
Аутори
Smiljković, MarijaStanisavljević, Danijela
Stojković, Dejan
Petrović, Isidora
Marjanović-Vicentić, Jelena
Popović, Jelena
Golic-Grdadolnik, Simona
Marković, Dejan
Sanković-Babić, Snežana
Glamočlija, Jasmina
Stevanović, Milena
Soković, Marina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both com...pounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
Кључне речи:
apigenin-7-O-glucoside / apigenin / antifungal / Candida spp. / cytotoxic / HCT116Извор:
EXCLI Journal, 2017, 16, 795-807Издавач:
- Excli Journal Managing Office, Dortmund
Финансирање / пројекти:
- Карактеризација и примена метаболита гљива и утврђивање потенцијала нових биофунгицида (RS-MESTD-Basic Research (BR or ON)-173032)
- Slovenian Research AgencySlovenian Research Agency - Slovenia [P1-0010]
- Programme of scientific and technological cooperation between the Republic of Serbia
- Republic of Slovenia, A combined methodology towards the development of novel, selective inhibitors of Candida [CYP51]
- Проучавање сигналних путева и епигенетичких механизама укључених у контролу експресије хуманих SOX гена: даље расветљавање њихове улоге у одређивању судбине и диференцијацији ћелија (RS-MESTD-Basic Research (BR or ON)-173051)
DOI: 10.17179/excli2017-300
ISSN: 1611-2156
PubMed: 28827996
WoS: 000406272100012
Scopus: 2-s2.0-85020933501
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Smiljković, Marija AU - Stanisavljević, Danijela AU - Stojković, Dejan AU - Petrović, Isidora AU - Marjanović-Vicentić, Jelena AU - Popović, Jelena AU - Golic-Grdadolnik, Simona AU - Marković, Dejan AU - Sanković-Babić, Snežana AU - Glamočlija, Jasmina AU - Stevanović, Milena AU - Soković, Marina PY - 2017 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2225 AB - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used. PB - Excli Journal Managing Office, Dortmund T2 - EXCLI Journal T1 - Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions VL - 16 SP - 795 EP - 807 DO - 10.17179/excli2017-300 ER -
@article{ author = "Smiljković, Marija and Stanisavljević, Danijela and Stojković, Dejan and Petrović, Isidora and Marjanović-Vicentić, Jelena and Popović, Jelena and Golic-Grdadolnik, Simona and Marković, Dejan and Sanković-Babić, Snežana and Glamočlija, Jasmina and Stevanović, Milena and Soković, Marina", year = "2017", abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.", publisher = "Excli Journal Managing Office, Dortmund", journal = "EXCLI Journal", title = "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions", volume = "16", pages = "795-807", doi = "10.17179/excli2017-300" }
Smiljković, M., Stanisavljević, D., Stojković, D., Petrović, I., Marjanović-Vicentić, J., Popović, J., Golic-Grdadolnik, S., Marković, D., Sanković-Babić, S., Glamočlija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal Excli Journal Managing Office, Dortmund., 16, 795-807. https://doi.org/10.17179/excli2017-300
Smiljković M, Stanisavljević D, Stojković D, Petrović I, Marjanović-Vicentić J, Popović J, Golic-Grdadolnik S, Marković D, Sanković-Babić S, Glamočlija J, Stevanović M, Soković M. Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807. doi:10.17179/excli2017-300 .
Smiljković, Marija, Stanisavljević, Danijela, Stojković, Dejan, Petrović, Isidora, Marjanović-Vicentić, Jelena, Popović, Jelena, Golic-Grdadolnik, Simona, Marković, Dejan, Sanković-Babić, Snežana, Glamočlija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807, https://doi.org/10.17179/excli2017-300 . .