P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population
2018
Аутори
Nikolić, NadjaČarkić, Jelena
Ilic-Dimitrijević, Ivana
Eljabo, Najib
Radunović, Milena
Aničić, Boban
Tanić, Nasta
Falk, Markus
Milašin, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P lt .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P lt .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively...). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.
Извор:
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 2018, 125, 1, 52-58Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
DOI: 10.1016/j.oooo.2017.09.013
ISSN: 2212-4403
PubMed: 29079368
WoS: 000417487000010
Scopus: 2-s2.0-85032179417
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Nikolić, Nadja AU - Čarkić, Jelena AU - Ilic-Dimitrijević, Ivana AU - Eljabo, Najib AU - Radunović, Milena AU - Aničić, Boban AU - Tanić, Nasta AU - Falk, Markus AU - Milašin, Jelena PY - 2018 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2317 AB - Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P lt .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P lt .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters. PB - Elsevier Science Inc, New York T2 - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology T1 - P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population VL - 125 IS - 1 SP - 52 EP - 58 DO - 10.1016/j.oooo.2017.09.013 ER -
@article{ author = "Nikolić, Nadja and Čarkić, Jelena and Ilic-Dimitrijević, Ivana and Eljabo, Najib and Radunović, Milena and Aničić, Boban and Tanić, Nasta and Falk, Markus and Milašin, Jelena", year = "2018", abstract = "Objective. To investigate the prevalence of p16(INK4) (a), p14(ARF), tumor protein p53 (TP53), and human telomerase reverse transcriptase (hTERT) promoter hypermethylation in mucoepidermoid carcinomas (MECs) and search for a possible association between methylation status and clinicopathological parameters. Study design. DNA extracted from 35 formalin-fixed and paraffin-embedded MEC samples and 10 normal salivary gland (NSG) tissue samples was analyzed for the presence of promoter hypermethylation using methylation-specific polymerase chain reaction testing. Results. The percentages of gene hypermethylation in MECs versus NSGs were the following: p14: 100% versus 20% (P lt .001); p16: 60% versus 20% (P = .035); hTERT: 54.3% versus 20% (P = .078); and TP53: 31.4% versus 30% (P = .981). Multiple sites were found to be methylated in 86% of MECs compared with 10% in NSGs (P lt .001). TP53 and hTERT were more often methylated in lower clinical stages (P = .033 and P = .005, respectively). Conclusions. Hypermethylation of p14 appears to be an important event in the development of mucoepidermoid carcinoma. High frequency of gene hypermethylation and high incidence of methylation at multiple sites point to the importance of epigenetic phenomena in the pathogenesis of MECs, although with modest impact on clinical parameters.", publisher = "Elsevier Science Inc, New York", journal = "Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology", title = "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population", volume = "125", number = "1", pages = "52-58", doi = "10.1016/j.oooo.2017.09.013" }
Nikolić, N., Čarkić, J., Ilic-Dimitrijević, I., Eljabo, N., Radunović, M., Aničić, B., Tanić, N., Falk, M.,& Milašin, J.. (2018). P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology Elsevier Science Inc, New York., 125(1), 52-58. https://doi.org/10.1016/j.oooo.2017.09.013
Nikolić N, Čarkić J, Ilic-Dimitrijević I, Eljabo N, Radunović M, Aničić B, Tanić N, Falk M, Milašin J. P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology. 2018;125(1):52-58. doi:10.1016/j.oooo.2017.09.013 .
Nikolić, Nadja, Čarkić, Jelena, Ilic-Dimitrijević, Ivana, Eljabo, Najib, Radunović, Milena, Aničić, Boban, Tanić, Nasta, Falk, Markus, Milašin, Jelena, "P14 methylation: an epigenetic signature of salivary gland mucoepidermoid carcinoma in the Serbian population" in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, 125, no. 1 (2018):52-58, https://doi.org/10.1016/j.oooo.2017.09.013 . .