Mesenchymal stem cells isolated from human periodontal ligament
2014
Аутори
Miletić, MajaMojsilović, S.
Okic-Đorđević, Ivana
Kukolj, Tamara
Jauković, Aleksandra
Santibanez, J. F.
Jovcić, Gordana
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells ...should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
Кључне речи:
Periodontal ligament / mesenchymal stem cells / characterization / proliferation / differentiation / tissue regenerationИзвор:
Archives of Biological Sciences, 2014, 66, 1, 261-271Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
- Примене нискотемпературних плазми у биомедицини, заштити човекове околине и нанотехнологијама (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41011)
DOI: 10.2298/ABS1401261M
ISSN: 0354-4664
WoS: 000330552400035
Scopus: 2-s2.0-84892417888
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Miletić, Maja AU - Mojsilović, S. AU - Okic-Đorđević, Ivana AU - Kukolj, Tamara AU - Jauković, Aleksandra AU - Santibanez, J. F. AU - Jovcić, Gordana AU - Bugarski, Diana PY - 2014 UR - https://smile.stomf.bg.ac.rs/handle/123456789/1951 AB - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Mesenchymal stem cells isolated from human periodontal ligament VL - 66 IS - 1 SP - 261 EP - 271 DO - 10.2298/ABS1401261M ER -
@article{ author = "Miletić, Maja and Mojsilović, S. and Okic-Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, J. F. and Jovcić, Gordana and Bugarski, Diana", year = "2014", abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Mesenchymal stem cells isolated from human periodontal ligament", volume = "66", number = "1", pages = "261-271", doi = "10.2298/ABS1401261M" }
Miletić, M., Mojsilović, S., Okic-Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovcić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271. https://doi.org/10.2298/ABS1401261M
Miletić M, Mojsilović S, Okic-Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovcić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271. doi:10.2298/ABS1401261M .
Miletić, Maja, Mojsilović, S., Okic-Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, J. F., Jovcić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271, https://doi.org/10.2298/ABS1401261M . .