Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
Само за регистроване кориснике
2015
Аутори
Karadžić, IvanaVučić, Vesna
Jokanović, Vukoman
Debeljak-Martačić, Jasmina
Marković, Dejan
Petrović, Snježana
Glibetić, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiat...ion and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.
Кључне речи:
dental pulp mesenchymal stem cells / hydroxyapatite / scaffolds / osteogenic differentiation / bone tissue engineeringИзвор:
Journal of Biomedical Materials Research Part A, 2015, 103, 1, 350-357Издавач:
- Wiley-Blackwell, Hoboken
Финансирање / пројекти:
- Биолошки механизми, нутритивни унос и статус полинезасићених масних киселина и фолата: Унапређење исхране у Србији (RS-41030)
- Хемијско и структурно дизајнирање наноматеријала за примену у медицини и инжењерству ткива (RS-172026)
DOI: 10.1002/jbm.a.35180
ISSN: 1549-3296
PubMed: 24665062
WoS: 000345572100039
Scopus: 2-s2.0-84912531491
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Karadžić, Ivana AU - Vučić, Vesna AU - Jokanović, Vukoman AU - Debeljak-Martačić, Jasmina AU - Marković, Dejan AU - Petrović, Snježana AU - Glibetić, Marija PY - 2015 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2063 AB - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. PB - Wiley-Blackwell, Hoboken T2 - Journal of Biomedical Materials Research Part A T1 - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells VL - 103 IS - 1 SP - 350 EP - 357 DO - 10.1002/jbm.a.35180 ER -
@article{ author = "Karadžić, Ivana and Vučić, Vesna and Jokanović, Vukoman and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija", year = "2015", abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of Biomedical Materials Research Part A", title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells", volume = "103", number = "1", pages = "350-357", doi = "10.1002/jbm.a.35180" }
Karadžić, I., Vučić, V., Jokanović, V., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A Wiley-Blackwell, Hoboken., 103(1), 350-357. https://doi.org/10.1002/jbm.a.35180
Karadžić I, Vučić V, Jokanović V, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research Part A. 2015;103(1):350-357. doi:10.1002/jbm.a.35180 .
Karadžić, Ivana, Vučić, Vesna, Jokanović, Vukoman, Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research Part A, 103, no. 1 (2015):350-357, https://doi.org/10.1002/jbm.a.35180 . .