Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
Само за регистроване кориснике
2017
Аутори
Ćetenović, BojanaProkić, Bogomir
Vasilijić, Saša
Dojčinović, Biljana
Magić, Marko
Jokanović, Vukoman
Marković, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPC...Al, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
Кључне речи:
Biocompatibility / biomaterials / calcium silicates / intraosseous implantation / MTA / MITИзвор:
Journal of Endodontics, 2017, 43, 3, 425-432Издавач:
- Elsevier Science Inc, New York
Финансирање / пројекти:
- Хемијско и структурно дизајнирање наноматеријала за примену у медицини и инжењерству ткива (RS-MESTD-Basic Research (BR or ON)-172026)
DOI: 10.1016/j.joen.2016.10.041
ISSN: 0099-2399
PubMed: 28231981
WoS: 000395971900011
Scopus: 2-s2.0-85013350258
Колекције
Институција/група
Stomatološki fakultetTY - JOUR AU - Ćetenović, Bojana AU - Prokić, Bogomir AU - Vasilijić, Saša AU - Dojčinović, Biljana AU - Magić, Marko AU - Jokanović, Vukoman AU - Marković, Dejan PY - 2017 UR - https://smile.stomf.bg.ac.rs/handle/123456789/2196 AB - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations. PB - Elsevier Science Inc, New York T2 - Journal of Endodontics T1 - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers VL - 43 IS - 3 SP - 425 EP - 432 DO - 10.1016/j.joen.2016.10.041 ER -
@article{ author = "Ćetenović, Bojana and Prokić, Bogomir and Vasilijić, Saša and Dojčinović, Biljana and Magić, Marko and Jokanović, Vukoman and Marković, Dejan", year = "2017", abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.", publisher = "Elsevier Science Inc, New York", journal = "Journal of Endodontics", title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers", volume = "43", number = "3", pages = "425-432", doi = "10.1016/j.joen.2016.10.041" }
Ćetenović, B., Prokić, B., Vasilijić, S., Dojčinović, B., Magić, M., Jokanović, V.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics Elsevier Science Inc, New York., 43(3), 425-432. https://doi.org/10.1016/j.joen.2016.10.041
Ćetenović B, Prokić B, Vasilijić S, Dojčinović B, Magić M, Jokanović V, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432. doi:10.1016/j.joen.2016.10.041 .
Ćetenović, Bojana, Prokić, Bogomir, Vasilijić, Saša, Dojčinović, Biljana, Magić, Marko, Jokanović, Vukoman, Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432, https://doi.org/10.1016/j.joen.2016.10.041 . .