TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Calvo Guirado, Jose Luis
AU  - Abboud, Marcus
AU  - Piedad Ramirez-Fernandez, Maria
AU  - Eduardo Mate-Sanchez, Jose
AU  - Negri, Bruno
AU  - Gomez-Moreno, Gerardo
AU  - Marković, Aleksa
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1970
AB  - PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light
VL  - 17
IS  - 4
SP  - 667
EP  - 680
DO  - 10.1111/cid.12161
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Calvo Guirado, Jose Luis and Abboud, Marcus and Piedad Ramirez-Fernandez, Maria and Eduardo Mate-Sanchez, Jose and Negri, Bruno and Gomez-Moreno, Gerardo and Marković, Aleksa",
year = "2015",
abstract = "PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light",
volume = "17",
number = "4",
pages = "667-680",
doi = "10.1111/cid.12161"
}

Delgado-Ruiz, R., Calvo Guirado, J. L., Abboud, M., Piedad Ramirez-Fernandez, M., Eduardo Mate-Sanchez, J., Negri, B., Gomez-Moreno, G.,& Marković, A.. (2015). Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 17(4), 667-680.
https://doi.org/10.1111/cid.12161

Delgado-Ruiz R, Calvo Guirado JL, Abboud M, Piedad Ramirez-Fernandez M, Eduardo Mate-Sanchez J, Negri B, Gomez-Moreno G, Marković A. Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research. 2015;17(4):667-680.
doi:10.1111/cid.12161 .

Delgado-Ruiz, Rafael, Calvo Guirado, Jose Luis, Abboud, Marcus, Piedad Ramirez-Fernandez, Maria, Eduardo Mate-Sanchez, Jose, Negri, Bruno, Gomez-Moreno, Gerardo, Marković, Aleksa, "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light" in Clinical Implant Dentistry & Related Research, 17, no. 4 (2015):667-680,
https://doi.org/10.1111/cid.12161 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Marković, Aleksa
AU  - Calvo Guirado, Jose Luis
AU  - Lazić, Zoran
AU  - Piattelli, Adriano
AU  - Boticelli, Daniele
AU  - Maté-Sánchez, José Eduardo
AU  - Negri, Bruno
AU  - Ramírez-Fernández, María Piedad
AU  - Mišić, Tijana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1900
AB  - Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels.
AB  - Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs
T1  - Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima
VL  - 71
IS  - 5
SP  - 451
EP  - 461
DO  - 10.2298/VSP121003034D
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Marković, Aleksa and Calvo Guirado, Jose Luis and Lazić, Zoran and Piattelli, Adriano and Boticelli, Daniele and Maté-Sánchez, José Eduardo and Negri, Bruno and Ramírez-Fernández, María Piedad and Mišić, Tijana",
year = "2014",
abstract = "Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels., Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs, Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima",
volume = "71",
number = "5",
pages = "451-461",
doi = "10.2298/VSP121003034D"
}

Delgado-Ruiz, R., Marković, A., Calvo Guirado, J. L., Lazić, Z., Piattelli, A., Boticelli, D., Maté-Sánchez, J. E., Negri, B., Ramírez-Fernández, M. P.,& Mišić, T.. (2014). Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(5), 451-461.
https://doi.org/10.2298/VSP121003034D

Delgado-Ruiz R, Marković A, Calvo Guirado JL, Lazić Z, Piattelli A, Boticelli D, Maté-Sánchez JE, Negri B, Ramírez-Fernández MP, Mišić T. Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled. 2014;71(5):451-461.
doi:10.2298/VSP121003034D .

Delgado-Ruiz, Rafael, Marković, Aleksa, Calvo Guirado, Jose Luis, Lazić, Zoran, Piattelli, Adriano, Boticelli, Daniele, Maté-Sánchez, José Eduardo, Negri, Bruno, Ramírez-Fernández, María Piedad, Mišić, Tijana, "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs" in Vojnosanitetski pregled, 71, no. 5 (2014):451-461,
https://doi.org/10.2298/VSP121003034D . .