@article{
author = "Petrović, Vanja and Pejčić, Nataša and Rakić, Mia and Leković, Vojislav and Vasić, Una and Stojić, Ž.",
year = "2012",
abstract = "Platelet-reach plasma (PRP) is an attractive tool in regenerative medicine due to its ability to stimulate proliferation and differentiation of stem cells. Since dental pulp derived stem cells are recognized as central in apexogenesis, the aim of the study was to evaluate radiologically and histologically effects of PRP on apexogenesis in teeth with immature roots. The study included eight monkeys (Cercopithecus Aethiops) divided in two equal groups for evaluation 3 and 12 months after treatment. All participants obtained the same treatment including pulpotomy and after-treatment with: hydroxiapatite (HA)-incisor and HA+canine PRP. Radiological evaluation was performed using the long cone paralleling technique for recording of defined parameters and histological evaluation was performed using tissue removed en block for the observation of parameters related to apexogenesis. The results obtained radiologically and histologically have shown increase in bridge formation in HA+PRP (75%) group after 3 months comparing to HA group (50%). Contrary to that, after 12 months there were no significant differences between groups. The root delay was not registered in the HA+PRP group contrary to HA group where it was registered in 25% after 12 months. Results of the study suggest that PRP is a powerful tool for intensive and rapid apexogenesis since it offers clear and comprehensive results (mostly in the first three months) which are early radiologically visible without any failure in the proposed requests., Primena plazme bogate trombocitima (PRP) predstavlja atraktivnu metodu u savremenoj regenerativnoj medicini zbog toga što ona ima sposobnost da stimuliš e proliferaciju i diferencijaciju stem ćelija. Kako su stem ćelije poreklom od dentalne pulpe definisane kao glavne u procesu apeksogeneze, cilj ove studije je bio da radiološki i histološki evaluira efekat PRP na apeksogenezu zuba sa nedovrš enim rastom korena. Studija je obuhvatila 8 majmuna (Cercopithecus Aethiops) koji su bili podeljeni u dve jednake grupe za evaluiranje, 3 i 12 meseci nakon terapije. Sve životinje su bile podvrgnute istom tretmanu uključujući pulpotomiju i tretman: hidroksiapatitom (HA) - sekutići i HA+PRP - očnjaci. Radiološka evaluacija je urađena određivanjem zadatih parametara na radiogramima dobijenim paralelnom tehnikom dugog konusa, a histološka analiza je sprovedena analiziranjem parametara svojstvenih apeksogenezi u preparatima uzetim 'en blok' tehnikom. Dobijeni rezultati su radiološki i histološki za dentinski most posle 3 meseca ukazali na porast u grupi HA+PRP (75%) u poređenju sa HA grupom (50%). Nasuprot tome, posle 12 meseci nije bilo razlike među grupama. Zaostatak u razvoju korena nije utvrđen u HA+PRP grupi za razliku od HA grupe gde je utvrđen zaostatak u 25% slučajeva. Rezultati studije navode na zaključak da je PRP moćno sredstvo za brzu i intezivnu apeksogenezu zbog toga što daje jasne i sveobuhvatne rezultate (najviše u prva tri meseca), koji su radiološki rano vidljivi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Effects of the platelet rich plasma on apexogenesis in young monkeys: Radiological and hystologycal evaluation, Efekti plazme bogate trombocitima na apeksogenezu kod mladih majmuna - radiološka i histološka evaluacija",
volume = "62",
number = "1",
pages = "39-52",
doi = "10.2298/AVB1201039P"
}
