TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Kontić-Vučinić, Olivera
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2572
AB  - Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.
PB  - Taylor & Francis Inc.
T2  - Hypertension in Pregnancy
T1  - VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia
VL  - 39
IS  - 4
SP  - 471
EP  - 480
DO  - 10.1080/10641955.2020.1843663
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Milašin, Jelena",
year = "2020",
abstract = "Objectives: Establishment of association between: (a) Val158Met COMT (G1947A) polymorphism and preeclampsia; (b) cytokines gene expression and COMT genotypes. Methods: 50 preeclampsia and 50 healthy pregnant women were enrolled. COMT genotyping was done by PCR/RFLP. TNF-α, IL-1β, and IL-6 mRNA levels were determined by Real-time PCR. Results: Variant (AA) homozygotes carried 3.7-fold increased preeclampsia odds, especially for severe (OR = 9.0, 95%CI (2.09-38.799)) and early forms (OR = 6.6, 95%CI (1.62-26.87)). AA homozygotes with PE had higher TNF-α levels compared to controls (P = 0.012). Conclusions: Val158Met COMT polymorphism increases preeclampsia risk. TNF-α expression and Val158Met COMT polymorphism have concomitant roles in PE pathogenesis.",
publisher = "Taylor & Francis Inc.",
journal = "Hypertension in Pregnancy",
title = "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia",
volume = "39",
number = "4",
pages = "471-480",
doi = "10.1080/10641955.2020.1843663"
}

Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J.,& Milašin, J.. (2020). VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy
Taylor & Francis Inc.., 39(4), 471-480.
https://doi.org/10.1080/10641955.2020.1843663

Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Milašin J. VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia. in Hypertension in Pregnancy. 2020;39(4):471-480.
doi:10.1080/10641955.2020.1843663 .

Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Milašin, Jelena, "VAL158MET catechol O-methyltransferase polymorphism contributes to the development of preeclampsia" in Hypertension in Pregnancy, 39, no. 4 (2020):471-480,
https://doi.org/10.1080/10641955.2020.1843663 . .

TY  - JOUR
AU  - Šljivančanin Jakovljević, Tamara
AU  - Kontić-Vučinić, Olivera
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Soldatović, Ivan
AU  - Milašin, Jelena
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2421
AB  - Problem Preeclampsia has a multifactorial origin with genetic, immunological, and environmental factors described as main contributors to its onset. This study aimed to investigate glutathione-S-transferase M1 (GSTM1) and glutathione-S-transferase T1 (GSTT1) gene polymorphisms, the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), and the potential relationship between GST polymorphisms and cytokine expression levels in preeclampsia and uncomplicated pregnancy. Method of Study This prospective case-control study included 50 women with preeclampsia and 50 healthy pregnant women. DNA and RNA were extracted from women leukocytes. Deletion polymorphisms were analyzed by PCR, while cytokine mRNA expression was analyzed by real-time PCR. Results GSTM1 null genotype with present GSTT1 increased the risk for preeclampsia development. Deletion of GSTT1 without deletion of GSTM1 increased the risk for early preeclampsia. Relative mRNA expression of TNF-alpha was significantly higher in preeclampsia compared to healthy pregnant women (P = 0.006). Expression of IL-1 beta was significantly higher in severe and late preeclampsia compared to the control group (P = 0.005, P = 0.007, respectively). A significant positive correlation between TNF-alpha and IL-1 beta was observed (Spearman's rho = 0.312, P = 0.028) and between IL-1 beta and IL-6, in preeclampsia group (Spearman's rho = 0.296, P = 0.037). IL-1 beta was significantly increased in patients with GSTT1 null genotype (P = 0.015) while IL-6 was increased in patients with GSTM1 null genotype (P = 0.015). Conclusions GSTM1 null genotype represents a risk factor for preeclampsia development, while GSTT1 null genotype favors early preeclampsia. Preeclampsia is also associated with increased expression of pro-inflammatory cytokines, predominantly TNF-alpha and IL-1 beta.
PB  - Wiley, Hoboken
T2  - American Journal of Reproductive Immunology
T1  - Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women
VL  - 81
IS  - 5
DO  - 10.1111/aji.13105
ER  - 

@article{
author = "Šljivančanin Jakovljević, Tamara and Kontić-Vučinić, Olivera and Nikolić, Nadja and Čarkić, Jelena and Soldatović, Ivan and Milašin, Jelena",
year = "2019",
abstract = "Problem Preeclampsia has a multifactorial origin with genetic, immunological, and environmental factors described as main contributors to its onset. This study aimed to investigate glutathione-S-transferase M1 (GSTM1) and glutathione-S-transferase T1 (GSTT1) gene polymorphisms, the expression of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), and the potential relationship between GST polymorphisms and cytokine expression levels in preeclampsia and uncomplicated pregnancy. Method of Study This prospective case-control study included 50 women with preeclampsia and 50 healthy pregnant women. DNA and RNA were extracted from women leukocytes. Deletion polymorphisms were analyzed by PCR, while cytokine mRNA expression was analyzed by real-time PCR. Results GSTM1 null genotype with present GSTT1 increased the risk for preeclampsia development. Deletion of GSTT1 without deletion of GSTM1 increased the risk for early preeclampsia. Relative mRNA expression of TNF-alpha was significantly higher in preeclampsia compared to healthy pregnant women (P = 0.006). Expression of IL-1 beta was significantly higher in severe and late preeclampsia compared to the control group (P = 0.005, P = 0.007, respectively). A significant positive correlation between TNF-alpha and IL-1 beta was observed (Spearman's rho = 0.312, P = 0.028) and between IL-1 beta and IL-6, in preeclampsia group (Spearman's rho = 0.296, P = 0.037). IL-1 beta was significantly increased in patients with GSTT1 null genotype (P = 0.015) while IL-6 was increased in patients with GSTM1 null genotype (P = 0.015). Conclusions GSTM1 null genotype represents a risk factor for preeclampsia development, while GSTT1 null genotype favors early preeclampsia. Preeclampsia is also associated with increased expression of pro-inflammatory cytokines, predominantly TNF-alpha and IL-1 beta.",
publisher = "Wiley, Hoboken",
journal = "American Journal of Reproductive Immunology",
title = "Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women",
volume = "81",
number = "5",
doi = "10.1111/aji.13105"
}

Šljivančanin Jakovljević, T., Kontić-Vučinić, O., Nikolić, N., Čarkić, J., Soldatović, I.,& Milašin, J.. (2019). Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women. in American Journal of Reproductive Immunology
Wiley, Hoboken., 81(5).
https://doi.org/10.1111/aji.13105

Šljivančanin Jakovljević T, Kontić-Vučinić O, Nikolić N, Čarkić J, Soldatović I, Milašin J. Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women. in American Journal of Reproductive Immunology. 2019;81(5).
doi:10.1111/aji.13105 .

Šljivančanin Jakovljević, Tamara, Kontić-Vučinić, Olivera, Nikolić, Nadja, Čarkić, Jelena, Soldatović, Ivan, Milašin, Jelena, "Glutathione-S-transferase M1 polymorphism and pro-inflammatory cytokines tumour necrosis factor-alpha and interleukin-1 beta are associated with preeclampsia in Serbian women" in American Journal of Reproductive Immunology, 81, no. 5 (2019),
https://doi.org/10.1111/aji.13105 . .