TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Lazarević, Miloš
AU  - Milošević-Marković, Maja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Milašin, Jelena
PY  - 2022
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3282
AB  - Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.
PB  - MDPI
T2  - Nanomaterials
T1  - The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla
VL  - 12
IS  - 18
SP  - 3116
DO  - 10.3390/nano12183116
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Lazarević, Miloš and Milošević-Marković, Maja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Milašin, Jelena",
year = "2022",
abstract = "Background: Dental stem cells, which originate from the neural crest, due to their easy
accessibility might be good candidates in neuro-regenerative procedures, along with graphene-based
nanomaterials shown to promote neurogenesis in vitro. We aimed to explore the potential of liquid-
phase exfoliated graphene (LPEG) film to stimulate the neuro-differentiation of stem cells from apical
papilla (SCAP). Methods: The experimental procedure was structured as follows: (1) fabrication
of graphene film; (2) isolation, cultivation and SCAP stemness characterization by flowcytometry,
multilineage differentiation (osteo, chondro and adipo) and quantitative PCR (qPCR); (3) SCAP
neuro-induction by cultivation on polyethylene terephthalate (PET) coated with graphene film;
(4) evaluation of neural differentiation by means of several microscopy techniques (light, confocal,
atomic force and scanning electron microscopy), followed by neural marker gene expression analysis
using qPCR. Results: SCAP demonstrated exceptional stemness, as judged by mesenchymal markers’
expression (CD73, CD90 and CD105), and by multilineage differentiation capacity (osteo, chondro and
adipo-differentiation). Neuro-induction of SCAP grown on PET coated with graphene film resulted
in neuron-like cellular phenotype observed under different microscopes. This was corroborated
by the high gene expression of all examined key neuronal markers (Ngn2, NF-M, Nestin, MAP2,
MASH1). Conclusions: The ability of SCAPs to differentiate toward neural lineages was markedly
enhanced by graphene film.",
publisher = "MDPI",
journal = "Nanomaterials",
title = "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla",
volume = "12",
number = "18",
pages = "3116",
doi = "10.3390/nano12183116"
}

Simonović, J., Toljić, B., Lazarević, M., Milošević-Marković, M., Perić, M., Vujin, J., Panajotović, R.,& Milašin, J.. (2022). The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials
MDPI., 12(18), 3116.
https://doi.org/10.3390/nano12183116

Simonović J, Toljić B, Lazarević M, Milošević-Marković M, Perić M, Vujin J, Panajotović R, Milašin J. The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla. in Nanomaterials. 2022;12(18):3116.
doi:10.3390/nano12183116 .

Simonović, Jelena, Toljić, Boško, Lazarević, Miloš, Milošević-Marković, Maja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Milašin, Jelena, "The Effect of Liquid-Phase Exfoliated Graphene Film on Neurodifferentiation of Stem Cells from Apical Papilla" in Nanomaterials, 12, no. 18 (2022):3116,
https://doi.org/10.3390/nano12183116 . .

TY  - JOUR
AU  - Simonović, Jelena
AU  - Toljić, Boško
AU  - Nikolić, Nadja
AU  - Perić, Mina
AU  - Vujin, Jasna
AU  - Panajotović, Radmila
AU  - Gajić, Radoš
AU  - Bekyarova, Elena
AU  - Cataldi, Amelia
AU  - Parpura, Vladimir
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2327
AB  - Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes
VL  - 106
IS  - 10
SP  - 2653
EP  - 2661
DO  - 10.1002/jbm.a.36461
ER  - 

@article{
author = "Simonović, Jelena and Toljić, Boško and Nikolić, Nadja and Perić, Mina and Vujin, Jasna and Panajotović, Radmila and Gajić, Radoš and Bekyarova, Elena and Cataldi, Amelia and Parpura, Vladimir and Milašin, Jelena",
year = "2018",
abstract = "Stem cell-based therapies are considered a promising treatment modality for many medical conditions. Several types of stem cells with variable differentiation potentials have been isolated from dental tissues, among them stem cells from apical papilla (SCAP). In parallel, new classes of biocompatible nanomaterials have also been developed, including graphene and carbon nanotube-based materials. The aim of the study was to assess whether graphene dispersion (GD) and water-soluble single walled carbon nanotubes (ws-SWCNT), may enhance SCAPs capacity to undergo neural differentiation. SCAPs cultivated in neuroinductive medium supplemented with GD and ws-SWCNT, separately and in combination, were subjected to neural marker analysis by real-time polymerase chain reaction (neurofilament medium [NF-M], neurogenin-2 [ngn-2], III-tubulin, microtubule-associated protein 2) and immunocytochemistry (NeuN and III-tubulin). GD, ws-SWCNT, and their combination, had neuro-stimulatory effects on SCAPs, as judged by the production of neural markers. Compared to cells grown in nanomaterial free medium, cells with GD showed higher production of B3T, cells with ws-SWCNT had higher production of ngn-2 and NF-M, while the combination of nanomaterials gave similar levels of both B3T and NF-M as the neuroinductive medium aloal ne, but with the finest neuron-like morphology. In conclusion, GD and ws-SWCNT seem to enhance neurdifferentiation of SCAP.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes",
volume = "106",
number = "10",
pages = "2653-2661",
doi = "10.1002/jbm.a.36461"
}

Simonović, J., Toljić, B., Nikolić, N., Perić, M., Vujin, J., Panajotović, R., Gajić, R., Bekyarova, E., Cataldi, A., Parpura, V.,& Milašin, J.. (2018). Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 106(10), 2653-2661.
https://doi.org/10.1002/jbm.a.36461

Simonović J, Toljić B, Nikolić N, Perić M, Vujin J, Panajotović R, Gajić R, Bekyarova E, Cataldi A, Parpura V, Milašin J. Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes. in Journal of Biomedical Materials Research Part A. 2018;106(10):2653-2661.
doi:10.1002/jbm.a.36461 .

Simonović, Jelena, Toljić, Boško, Nikolić, Nadja, Perić, Mina, Vujin, Jasna, Panajotović, Radmila, Gajić, Radoš, Bekyarova, Elena, Cataldi, Amelia, Parpura, Vladimir, Milašin, Jelena, "Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes" in Journal of Biomedical Materials Research Part A, 106, no. 10 (2018):2653-2661,
https://doi.org/10.1002/jbm.a.36461 . .