TY  - JOUR
AU  - Velicković, Milena
AU  - Pejnović, Nada
AU  - Petrović, Renata
AU  - Mitrović, Slobodanka
AU  - Jeftić, Ilija
AU  - Kanjevac, Tatjana
AU  - Lukić, Aleksandra
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2135
AB  - BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P  lt  0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.
PB  - Wiley, Hoboken
T2  - Journal of Oral Pathology & Medicine
T1  - Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts
VL  - 45
IS  - 1
SP  - 70
EP  - 76
DO  - 10.1111/jop.12312
ER  - 

@article{
author = "Velicković, Milena and Pejnović, Nada and Petrović, Renata and Mitrović, Slobodanka and Jeftić, Ilija and Kanjevac, Tatjana and Lukić, Aleksandra",
year = "2016",
abstract = "BACKGROUND: Interleukin-33 (IL-33) is a recently identified cytokine belonging to the IL-1 family and ligand for the IL-1 receptor-related protein ST2. IL-33/ST2 signaling plays a critical role in allergy, autoimmunity, and chronic inflammatory disorders, but its role in the pathogenesis of periapical lesions is unknown. We aimed to investigate the expression patterns of IL-33 and ST2 in human periapical lesions. METHODS: Periapical lesions (n = 36) and healthy periapical tissues (n = 10) were evaluated by immunohistochemistry using antibodies specific for human IL-33 and ST2. Lesion samples were further analyzed by double immunofluorescence to assess IL-33/ST2 co-expression. RESULTS: The numbers of IL-33- and ST2-positive fibroblasts were significantly higher in periapical lesions compared to healthy periapical tissues (both P  lt  0.05), while the numbers of IL-33-and ST2-positive endothelial cells were similar (both P > 0.05). There were no significant differences in the numbers of IL-33-and ST2-positive fibroblasts and endothelial cells between periapical granulomas and radicular cysts (all P > 0.05). Similarly, numbers of ST2-positive mononuclear cells did not differ between periapical granulomas and radicular cysts (P > 0.05). The majority of epithelial cells in radicular cysts were IL-33 positive, while the small proportion of epithelial cells was ST2 positive. Double immunofluorescence analysis revealed IL-33/ST2 co-expression in fibroblasts and endothelial cells. CONCLUSIONS: IL-33 and ST2 are expressed in periapical granulomas and radicular cysts. Increased numbers of IL-33-and ST2-positive fibroblasts in periapical lesions when compared to healthy periapical tissues suggest that IL-33/ST2 signaling may be involved in periapical inflammation and tissue fibrosis.",
publisher = "Wiley, Hoboken",
journal = "Journal of Oral Pathology & Medicine",
title = "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts",
volume = "45",
number = "1",
pages = "70-76",
doi = "10.1111/jop.12312"
}

Velicković, M., Pejnović, N., Petrović, R., Mitrović, S., Jeftić, I., Kanjevac, T.,& Lukić, A.. (2016). Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine
Wiley, Hoboken., 45(1), 70-76.
https://doi.org/10.1111/jop.12312

Velicković M, Pejnović N, Petrović R, Mitrović S, Jeftić I, Kanjevac T, Lukić A. Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts. in Journal of Oral Pathology & Medicine. 2016;45(1):70-76.
doi:10.1111/jop.12312 .

Velicković, Milena, Pejnović, Nada, Petrović, Renata, Mitrović, Slobodanka, Jeftić, Ilija, Kanjevac, Tatjana, Lukić, Aleksandra, "Expression of interleukin-33 and its receptor ST2 in periapical granulomas and radicular cysts" in Journal of Oral Pathology & Medicine, 45, no. 1 (2016):70-76,
https://doi.org/10.1111/jop.12312 . .