TY  - JOUR
AU  - Andjus, Stefan
AU  - Nikolić, Nadja
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Lazović, Vladimir
AU  - Tubić, Bojana
AU  - Čanak-Atlagić, Jelena
AU  - Nikolić, Vera
AU  - Paunović, Momir
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2381
AB  - Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.
PB  - Elsevier Gmbh, Munich
T2  - Limnologica
T1  - First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study
VL  - 76
SP  - 48
EP  - 51
DO  - 10.1016/j.limno.2019.03.004
ER  - 

@article{
author = "Andjus, Stefan and Nikolić, Nadja and Marjanović, Ana and Branković, Marija and Lazović, Vladimir and Tubić, Bojana and Čanak-Atlagić, Jelena and Nikolić, Vera and Paunović, Momir",
year = "2019",
abstract = "Trochospongilla horrida Weltner, 1893 (Spongillidae, Spongillida) has a relatively wide, though discontinuous, distribution in the Northern Hemisphere. The aim of this paper was to report the first finding of T. horrida in Serbia. During 2017, research was carried out on ten tributaries of the Sava and the Danube. T. horrida was identified at three localities in Tisa, Juzna Morava and Zapadna Morava rivers, out of 51 inspected sites. For the first time ribosomal 28S DNA was used for T. horrida identification.",
publisher = "Elsevier Gmbh, Munich",
journal = "Limnologica",
title = "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study",
volume = "76",
pages = "48-51",
doi = "10.1016/j.limno.2019.03.004"
}

Andjus, S., Nikolić, N., Marjanović, A., Branković, M., Lazović, V., Tubić, B., Čanak-Atlagić, J., Nikolić, V.,& Paunović, M.. (2019). First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study. in Limnologica
Elsevier Gmbh, Munich., 76, 48-51.
https://doi.org/10.1016/j.limno.2019.03.004

Andjus S, Nikolić N, Marjanović A, Branković M, Lazović V, Tubić B, Čanak-Atlagić J, Nikolić V, Paunović M. First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study. in Limnologica. 2019;76:48-51.
doi:10.1016/j.limno.2019.03.004 .

Andjus, Stefan, Nikolić, Nadja, Marjanović, Ana, Branković, Marija, Lazović, Vladimir, Tubić, Bojana, Čanak-Atlagić, Jelena, Nikolić, Vera, Paunović, Momir, "First record of freshwater sponge Trochospongilla horrida Weltner, 1893 in Serbia - A morphological and genetic study" in Limnologica, 76 (2019):48-51,
https://doi.org/10.1016/j.limno.2019.03.004 . .

TY  - JOUR
AU  - Trifunović, Jovanka
AU  - Basta-Jovanović, Gordana
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Radojević-Škodrić, Sanja
AU  - Prvanović, Mirjana
AU  - Jovanović, Aleksandar
AU  - Džamić, Zoran
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2272
AB  - Purpose: Renal cell carcinoma (RCC) is the most common renal cancer in adults and includes several subtypes that may be distinguished by their histology, genetic background, clinical course and responses to treatment. Human telomerase reverse transcriptase (hTERT), a crucial enzyme for telomere maintenance, has been linked to RCC development. The purpose of this study was to search for genetic and epigenetic alterations in hTERT (promoter mutations and methylation and gene amplification), and to establish a possible association between molecular and clinico-pathological characteristics of RCC. Methods: DNA was extracted from 31 formalin fixed, para,, n-embedded tumor samples and 23 blood samples from 54 patients with RCC. Polymerase chain reaction (PCR) products were sequenced and analyzed using the Sequencher software. HTERT amplification was determined by quantitative PCR, while the promoter methylation status was assessed by methylation specific PCR. Statistical analysis was performed using SPSS. Results: No mutations could be detected in the hTERT promoter but only a single nucleotide polymorphism (SNP) (-245 T > C). In 54 analyzed RCC cases, the variant allele C was present in homozygous or heterozygous form in 48% of the patients. The C allele was significantly more frequent in low grade tumors (p=0.046). Gene amplification was detected in 19.4% of the 31 RCCs and hTERT methylation in 54.8% of the 31 samples. An association was established between methylation and histological type of RCC (p=0.047). Conclusions: HTERT seems to be implicated in RCC pathogenesis since the promoter polymorphism exerts a modulation effect on tumor behavior. In addition, hTERT promoter methylation status is related to RCC histology.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population
VL  - 23
IS  - 6
SP  - 1887
EP  - 1892
UR  - https://hdl.handle.net/21.15107/rcub_smile_2272
ER  - 

@article{
author = "Trifunović, Jovanka and Basta-Jovanović, Gordana and Nikolić, Nadja and Čarkić, Jelena and Marjanović, Ana and Branković, Marija and Radojević-Škodrić, Sanja and Prvanović, Mirjana and Jovanović, Aleksandar and Džamić, Zoran and Milašin, Jelena",
year = "2018",
abstract = "Purpose: Renal cell carcinoma (RCC) is the most common renal cancer in adults and includes several subtypes that may be distinguished by their histology, genetic background, clinical course and responses to treatment. Human telomerase reverse transcriptase (hTERT), a crucial enzyme for telomere maintenance, has been linked to RCC development. The purpose of this study was to search for genetic and epigenetic alterations in hTERT (promoter mutations and methylation and gene amplification), and to establish a possible association between molecular and clinico-pathological characteristics of RCC. Methods: DNA was extracted from 31 formalin fixed, para,, n-embedded tumor samples and 23 blood samples from 54 patients with RCC. Polymerase chain reaction (PCR) products were sequenced and analyzed using the Sequencher software. HTERT amplification was determined by quantitative PCR, while the promoter methylation status was assessed by methylation specific PCR. Statistical analysis was performed using SPSS. Results: No mutations could be detected in the hTERT promoter but only a single nucleotide polymorphism (SNP) (-245 T > C). In 54 analyzed RCC cases, the variant allele C was present in homozygous or heterozygous form in 48% of the patients. The C allele was significantly more frequent in low grade tumors (p=0.046). Gene amplification was detected in 19.4% of the 31 RCCs and hTERT methylation in 54.8% of the 31 samples. An association was established between methylation and histological type of RCC (p=0.047). Conclusions: HTERT seems to be implicated in RCC pathogenesis since the promoter polymorphism exerts a modulation effect on tumor behavior. In addition, hTERT promoter methylation status is related to RCC histology.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population",
volume = "23",
number = "6",
pages = "1887-1892",
url = "https://hdl.handle.net/21.15107/rcub_smile_2272"
}

Trifunović, J., Basta-Jovanović, G., Nikolić, N., Čarkić, J., Marjanović, A., Branković, M., Radojević-Škodrić, S., Prvanović, M., Jovanović, A., Džamić, Z.,& Milašin, J.. (2018). HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(6), 1887-1892.
https://hdl.handle.net/21.15107/rcub_smile_2272

Trifunović J, Basta-Jovanović G, Nikolić N, Čarkić J, Marjanović A, Branković M, Radojević-Škodrić S, Prvanović M, Jovanović A, Džamić Z, Milašin J. HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population. in Journal of BUON. 2018;23(6):1887-1892.
https://hdl.handle.net/21.15107/rcub_smile_2272 .

Trifunović, Jovanka, Basta-Jovanović, Gordana, Nikolić, Nadja, Čarkić, Jelena, Marjanović, Ana, Branković, Marija, Radojević-Škodrić, Sanja, Prvanović, Mirjana, Jovanović, Aleksandar, Džamić, Zoran, Milašin, Jelena, "HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population" in Journal of BUON, 23, no. 6 (2018):1887-1892,
https://hdl.handle.net/21.15107/rcub_smile_2272 .