TY  - JOUR
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Jelovac, Drago
AU  - Milenković, Sanja
AU  - Tepavčević, Zvezdana
AU  - Baldan, Federica
AU  - Suboticki, Tijana
AU  - Toljić, Boško
AU  - Trišić, Dijana
AU  - Dragović, Miroslav
AU  - Damante, Giuseppe
AU  - Milašin, Jelena
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2484
AB  - Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.
PB  - SPANDIDOS PUBL LTD, ATHENS
T2  - Oncology Letters
T1  - Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study
VL  - 19
IS  - 6
SP  - 3743
EP  - 3750
DO  - 10.3892/ol.2020.11494
ER  - 

@article{
author = "Lazarević, Miloš and Milošević, Maja and Jelovac, Drago and Milenković, Sanja and Tepavčević, Zvezdana and Baldan, Federica and Suboticki, Tijana and Toljić, Boško and Trišić, Dijana and Dragović, Miroslav and Damante, Giuseppe and Milašin, Jelena",
year = "2020",
abstract = "Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, alpha -smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics.",
publisher = "SPANDIDOS PUBL LTD, ATHENS",
journal = "Oncology Letters",
title = "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study",
volume = "19",
number = "6",
pages = "3743-3750",
doi = "10.3892/ol.2020.11494"
}

Lazarević, M., Milošević, M., Jelovac, D., Milenković, S., Tepavčević, Z., Baldan, F., Suboticki, T., Toljić, B., Trišić, D., Dragović, M., Damante, G.,& Milašin, J.. (2020). Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters
SPANDIDOS PUBL LTD, ATHENS., 19(6), 3743-3750.
https://doi.org/10.3892/ol.2020.11494

Lazarević M, Milošević M, Jelovac D, Milenković S, Tepavčević Z, Baldan F, Suboticki T, Toljić B, Trišić D, Dragović M, Damante G, Milašin J. Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. in Oncology Letters. 2020;19(6):3743-3750.
doi:10.3892/ol.2020.11494 .

Lazarević, Miloš, Milošević, Maja, Jelovac, Drago, Milenković, Sanja, Tepavčević, Zvezdana, Baldan, Federica, Suboticki, Tijana, Toljić, Boško, Trišić, Dijana, Dragović, Miroslav, Damante, Giuseppe, Milašin, Jelena, "Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study" in Oncology Letters, 19, no. 6 (2020):3743-3750,
https://doi.org/10.3892/ol.2020.11494 . .

TY  - JOUR
AU  - Paraš, S.
AU  - Trišić, Dijana
AU  - Ajtić, O.M.
AU  - Prokić, B.
AU  - Drobne, D.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2478
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
PB  - Mdpi Ag
T2  - Nanomaterials
T1  - Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats
VL  - 10
IS  - 5
DO  - 10.3390/nano10050918
ER  - 

@article{
author = "Paraš, S. and Trišić, Dijana and Ajtić, O.M. and Prokić, B. and Drobne, D. and Živković, Slavoljub and Jokanović, Vukoman",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
publisher = "Mdpi Ag",
journal = "Nanomaterials",
title = "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats",
volume = "10",
number = "5",
doi = "10.3390/nano10050918"
}

Paraš, S., Trišić, D., Ajtić, O.M., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V.. (2020). Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials
Mdpi Ag., 10(5).
https://doi.org/10.3390/nano10050918

Paraš S, Trišić D, Ajtić O, Prokić B, Drobne D, Živković S, Jokanović V. Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials. 2020;10(5).
doi:10.3390/nano10050918 .

Paraš, S., Trišić, Dijana, Ajtić, O.M., Prokić, B., Drobne, D., Živković, Slavoljub, Jokanović, Vukoman, "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats" in Nanomaterials, 10, no. 5 (2020),
https://doi.org/10.3390/nano10050918 . .

TY  - JOUR
AU  - Paras, S.
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Mitrović-Ajtić, O.
AU  - Dekić, R.
AU  - Soldatović, Ivan
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2393
AB  - Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
VL  - 52
IS  - 8
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 

@article{
author = "Paras, S. and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana and Mitrović-Ajtić, O. and Dekić, R. and Soldatović, Ivan and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman",
year = "2019",
abstract = "Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
volume = "52",
number = "8",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}

Paras, S., Janković, O., Trišić, D., Čolović, B., Mitrović-Ajtić, O., Dekić, R., Soldatović, I., Živković-Sandić, M., Živković, S.,& Jokanović, V.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal
Wiley, Hoboken., 52(8), 1162-1172.
https://doi.org/10.1111/iej.13105

Paras S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković-Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;52(8):1162-1172.
doi:10.1111/iej.13105 .

Paras, S., Janković, Ognjenka, Trišić, Dijana, Čolović, Božana, Mitrović-Ajtić, O., Dekić, R., Soldatović, Ivan, Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman, "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal, 52, no. 8 (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .

TY  - THES
AU  - Trišić, Dijana
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7249
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20993/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51890703
UR  - http://nardus.mpn.gov.rs/123456789/12096
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1041
AB  - Stem cells from apical papilla (SCAPs) represent a homogeneous cell population which differentiates into primary odontoblasts and form primary root dentine. Young permanent teeth, more prone to dental trauma and developmental anomalies, have greater susceptibility to develop endodontic infections. During endodontic treatment of young permanent teeth, control of infection represents a necessary step in creating conditions for further tissue regeneration and healing. Due to good antibacterial potential, the application of photodynamic therapy and a high-power diode laser has been recommended, as addition to standard endodontic protocol. The Aim of this study was to investigate biocompatibility of diode laser and photodynamic therapy on stem cells from apical papilla, as well as the antibacterial effect of standard disinfection, lasers, and materials for definitive root canal obturation in endodontic treatment of young permanent teeth. Materials and methods: SCAPs were isolated and characterized by internationally accepted criteria for mesenchymal stem cells. Biocompatibility of diode laser and photodynamic therapy was investigated on characterized stem cells by the mitochondrial activity assay (MTT assay) and cells viability assay (Neutral red assay) after 1 hour, 1 and 7 days. Lasers bbiostimulation effect on cells migration was evaluated by scratch assay, while the effect on osteogenic differentiation was investigated by measurement of alkaline phosphatase (3 7, and 14 days) and Alizarin red staining. Relative gene expression analysis and protein staining of selected markers for odonto/osteogenic differentiation, have been performed as well after 21 days. In a randomized controlled clinical study, patients have been relocated in 3 study groups. Antibacterial effect was evaluated after standard endodontic treatment, as well as after diode laser application and photodynamic therapy, in corresponding groups. Samples were seeded on microbial plates, and additionally analyzed by molecular methods. Antibacterial effects of calcium hydroxide, mineral trioxide aggregate, and calcium silicate with the addition of bismuth oxide were investigated on clinical isolates, by the method of double agar diffusion test in three time periods (1, 3, and 24 hours)...
AB  - Uvod: Matične ćelije poreklom iz apikalne papile (SCAP) predstavljaju homogenu ćelijsku populaciju koja diferentuje u primarne odontoblaste i formira primarni dentin korena. Mladi stalni zubi, usled sklonosti ka povredama i razvojnim anomalijama, su u velikom riziku za nastanak endodontskih oboljenja. Tokom endodontskog lečenja mladih stalnih zuba kontrola infekcije predstavlja neophodan korak u stvaranju uslova za regeneraciju i zarastanje. Usled dobrog antibakterijskog potencijala, predložena je upotreba fotodinamske terapije i diodnih lasera velike snage u endodontskom lečenju, kao dodatak važećim protokolima. Cilj ovog istraživanja bio je da se ispita biokompatibilnost diodnog lasera i fotodinamske terapije na matičnim ćelijama poreklom iz apikalne papile zuba, kao i antimikrobno dejstvo tradicionalnih antimikrobnih sredstava, lasera, i materijala za definitivnu opturaciju kanala korena u endodontskom lečenju mladih stalnih zuba. Materijali i metode: Ćelije iz apikalne papile su izolovane i okarakterisane prema međunarodno definisanim kriterijumima za mezenhimalne matične ćelije. Na okarakterisanim matičnim ćelijama poreklom iz apikalne papile ispitana je biokompatibilnost diodnog lasera i fotodinamske terapije testom mitohondrijske aktivnosti (MTT) i direktnim testom vijabilnosti ćelija (Neutralno crveno) nakon 1 sata, 1 i 7 dana. Biostimulativno dejstvo lasera na potencijal migracije ispitano je testom ogrebotine, dok je uticaj na potencijal osteogenog usmeravanja ispitana merenjem alkalne fosfataze (3, 7 i 14 dana) i bojenjem Alizarin crvenim. Ispitan je i nivo relativne genske ekspresije i specifično bojenje proteina za markere odonto/osteogene diferencijacije, nakon 21 dana. U randomizovanoj kontrolisanoj kliničkoj studiji, pacijenti su raspoređeni u 3 ogledne grupe. Antimikrobno dejstvo je ispitano nakon dejstva standardnih antimikrobnih sredstava, kao i nakon dodatnog dejstva diodnog lasera i fotodinamske terapije u odgovarajućim grupama...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla
T1  - Uticaj fotodinamske terapije i diodnog lasera na uspešnost endodontskog lečenja mladih stalnih zuba i biostimulaciju matičnih ćelija poreklom iz apikalne papile
UR  - https://hdl.handle.net/21.15107/rcub_nardus_12096
ER  - 

@phdthesis{
author = "Trišić, Dijana",
year = "2019",
abstract = "Stem cells from apical papilla (SCAPs) represent a homogeneous cell population which differentiates into primary odontoblasts and form primary root dentine. Young permanent teeth, more prone to dental trauma and developmental anomalies, have greater susceptibility to develop endodontic infections. During endodontic treatment of young permanent teeth, control of infection represents a necessary step in creating conditions for further tissue regeneration and healing. Due to good antibacterial potential, the application of photodynamic therapy and a high-power diode laser has been recommended, as addition to standard endodontic protocol. The Aim of this study was to investigate biocompatibility of diode laser and photodynamic therapy on stem cells from apical papilla, as well as the antibacterial effect of standard disinfection, lasers, and materials for definitive root canal obturation in endodontic treatment of young permanent teeth. Materials and methods: SCAPs were isolated and characterized by internationally accepted criteria for mesenchymal stem cells. Biocompatibility of diode laser and photodynamic therapy was investigated on characterized stem cells by the mitochondrial activity assay (MTT assay) and cells viability assay (Neutral red assay) after 1 hour, 1 and 7 days. Lasers bbiostimulation effect on cells migration was evaluated by scratch assay, while the effect on osteogenic differentiation was investigated by measurement of alkaline phosphatase (3 7, and 14 days) and Alizarin red staining. Relative gene expression analysis and protein staining of selected markers for odonto/osteogenic differentiation, have been performed as well after 21 days. In a randomized controlled clinical study, patients have been relocated in 3 study groups. Antibacterial effect was evaluated after standard endodontic treatment, as well as after diode laser application and photodynamic therapy, in corresponding groups. Samples were seeded on microbial plates, and additionally analyzed by molecular methods. Antibacterial effects of calcium hydroxide, mineral trioxide aggregate, and calcium silicate with the addition of bismuth oxide were investigated on clinical isolates, by the method of double agar diffusion test in three time periods (1, 3, and 24 hours)..., Uvod: Matične ćelije poreklom iz apikalne papile (SCAP) predstavljaju homogenu ćelijsku populaciju koja diferentuje u primarne odontoblaste i formira primarni dentin korena. Mladi stalni zubi, usled sklonosti ka povredama i razvojnim anomalijama, su u velikom riziku za nastanak endodontskih oboljenja. Tokom endodontskog lečenja mladih stalnih zuba kontrola infekcije predstavlja neophodan korak u stvaranju uslova za regeneraciju i zarastanje. Usled dobrog antibakterijskog potencijala, predložena je upotreba fotodinamske terapije i diodnih lasera velike snage u endodontskom lečenju, kao dodatak važećim protokolima. Cilj ovog istraživanja bio je da se ispita biokompatibilnost diodnog lasera i fotodinamske terapije na matičnim ćelijama poreklom iz apikalne papile zuba, kao i antimikrobno dejstvo tradicionalnih antimikrobnih sredstava, lasera, i materijala za definitivnu opturaciju kanala korena u endodontskom lečenju mladih stalnih zuba. Materijali i metode: Ćelije iz apikalne papile su izolovane i okarakterisane prema međunarodno definisanim kriterijumima za mezenhimalne matične ćelije. Na okarakterisanim matičnim ćelijama poreklom iz apikalne papile ispitana je biokompatibilnost diodnog lasera i fotodinamske terapije testom mitohondrijske aktivnosti (MTT) i direktnim testom vijabilnosti ćelija (Neutralno crveno) nakon 1 sata, 1 i 7 dana. Biostimulativno dejstvo lasera na potencijal migracije ispitano je testom ogrebotine, dok je uticaj na potencijal osteogenog usmeravanja ispitana merenjem alkalne fosfataze (3, 7 i 14 dana) i bojenjem Alizarin crvenim. Ispitan je i nivo relativne genske ekspresije i specifično bojenje proteina za markere odonto/osteogene diferencijacije, nakon 21 dana. U randomizovanoj kontrolisanoj kliničkoj studiji, pacijenti su raspoređeni u 3 ogledne grupe. Antimikrobno dejstvo je ispitano nakon dejstva standardnih antimikrobnih sredstava, kao i nakon dodatnog dejstva diodnog lasera i fotodinamske terapije u odgovarajućim grupama...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla, Uticaj fotodinamske terapije i diodnog lasera na uspešnost endodontskog lečenja mladih stalnih zuba i biostimulaciju matičnih ćelija poreklom iz apikalne papile",
url = "https://hdl.handle.net/21.15107/rcub_nardus_12096"
}

Trišić, D.. (2019). Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_12096

Trišić D. Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_12096 .

Trišić, Dijana, "Influence of photodynamic therapy and diode laser on successful endodontic treatment of young permanent teeth and biostimulation of stem cells from apical papilla" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_12096 .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2444
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
AB  - Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution., Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}

Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T

Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .

Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .

TY  - JOUR
AU  - Marković, Dejan
AU  - Petrović, Bojan
AU  - Perić, Tamara
AU  - Trišić, Dijana
AU  - Kojić, Sanja
AU  - Kuljić, Božidar L.
AU  - Stojanović, Goran
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2376
AB  - Purpose: The objective was to assess the effect of fissure depth and enamel surface preparation protocols on penetration of resin-based, giomer and glass-ionomer fissure sealants. Materials and Methods: Ninety extracted third molars were assigned into three groups according to the material used. All three groups were subsequently divided depending on tooth preparation: no pretreatment or surface preparation with 10% polyacrylic acid, 37% phosphoric acid, or self-etching adhesive. In addition, Er,Cr:YSGG laser or air abrasion were employed in all subgroups. The penetration was evaluated using scanning electron microscopy. Results: The groups that were pretreated with an appropriate acid resulted in superior penetration of glass-ionomer and resin-based sealants when compared with other surface preparation protocols (p  lt  0.05). The exception was teeth treated with both air abrasion and acid etching. Both preparation protocol and fissure depth significantly affected the sealant penetration (p  lt  0.05), but fissure depth had a greater impact on fissure penetration than did enamel surface pretreatment. Conclusion: All investigated materials exhibit similar properties regarding sealant penetration. Penetration of a fissure sealant is significantly influenced by the fissure depth. In regard to enamel surface preparation protocol, a pretreatment with phosphoric acid in resin-based and polyacrylic acid in glass-ionomers appears to be essential in obtaining the adequate penetration of a sealing material.
PB  - Quintessence Publishing Co Inc, Hanover Park
T2  - Oral Health & Preventive Dentistry
T1  - Evaluation of Sealant Penetration in Relation to Fissure Morphology, Enamel Surface Preparation Protocol and Sealing Material
VL  - 17
IS  - 4
SP  - 349
EP  - 355
DO  - 10.3290/j.ohpd.a42689
ER  - 

@article{
author = "Marković, Dejan and Petrović, Bojan and Perić, Tamara and Trišić, Dijana and Kojić, Sanja and Kuljić, Božidar L. and Stojanović, Goran",
year = "2019",
abstract = "Purpose: The objective was to assess the effect of fissure depth and enamel surface preparation protocols on penetration of resin-based, giomer and glass-ionomer fissure sealants. Materials and Methods: Ninety extracted third molars were assigned into three groups according to the material used. All three groups were subsequently divided depending on tooth preparation: no pretreatment or surface preparation with 10% polyacrylic acid, 37% phosphoric acid, or self-etching adhesive. In addition, Er,Cr:YSGG laser or air abrasion were employed in all subgroups. The penetration was evaluated using scanning electron microscopy. Results: The groups that were pretreated with an appropriate acid resulted in superior penetration of glass-ionomer and resin-based sealants when compared with other surface preparation protocols (p  lt  0.05). The exception was teeth treated with both air abrasion and acid etching. Both preparation protocol and fissure depth significantly affected the sealant penetration (p  lt  0.05), but fissure depth had a greater impact on fissure penetration than did enamel surface pretreatment. Conclusion: All investigated materials exhibit similar properties regarding sealant penetration. Penetration of a fissure sealant is significantly influenced by the fissure depth. In regard to enamel surface preparation protocol, a pretreatment with phosphoric acid in resin-based and polyacrylic acid in glass-ionomers appears to be essential in obtaining the adequate penetration of a sealing material.",
publisher = "Quintessence Publishing Co Inc, Hanover Park",
journal = "Oral Health & Preventive Dentistry",
title = "Evaluation of Sealant Penetration in Relation to Fissure Morphology, Enamel Surface Preparation Protocol and Sealing Material",
volume = "17",
number = "4",
pages = "349-355",
doi = "10.3290/j.ohpd.a42689"
}

Marković, D., Petrović, B., Perić, T., Trišić, D., Kojić, S., Kuljić, B. L.,& Stojanović, G.. (2019). Evaluation of Sealant Penetration in Relation to Fissure Morphology, Enamel Surface Preparation Protocol and Sealing Material. in Oral Health & Preventive Dentistry
Quintessence Publishing Co Inc, Hanover Park., 17(4), 349-355.
https://doi.org/10.3290/j.ohpd.a42689

Marković D, Petrović B, Perić T, Trišić D, Kojić S, Kuljić BL, Stojanović G. Evaluation of Sealant Penetration in Relation to Fissure Morphology, Enamel Surface Preparation Protocol and Sealing Material. in Oral Health & Preventive Dentistry. 2019;17(4):349-355.
doi:10.3290/j.ohpd.a42689 .

Marković, Dejan, Petrović, Bojan, Perić, Tamara, Trišić, Dijana, Kojić, Sanja, Kuljić, Božidar L., Stojanović, Goran, "Evaluation of Sealant Penetration in Relation to Fissure Morphology, Enamel Surface Preparation Protocol and Sealing Material" in Oral Health & Preventive Dentistry, 17, no. 4 (2019):349-355,
https://doi.org/10.3290/j.ohpd.a42689 . .

TY  - JOUR
AU  - Lazarević, Miloš
AU  - Milošević, Maja
AU  - Trišić, Dijana
AU  - Toljić, Boško
AU  - Simonović, Jelena
AU  - Nikolić, Nadja
AU  - Miković, Nikola
AU  - Jelovac, Drago
AU  - Petrović, Milan
AU  - Vukadinović, Miroslav
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2271
AB  - Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma
VL  - 23
IS  - 6
SP  - 1686
EP  - 1692
UR  - https://hdl.handle.net/21.15107/rcub_smile_2271
ER  - 

@article{
author = "Lazarević, Miloš and Milošević, Maja and Trišić, Dijana and Toljić, Boško and Simonović, Jelena and Nikolić, Nadja and Miković, Nikola and Jelovac, Drago and Petrović, Milan and Vukadinović, Miroslav and Milašin, Jelena",
year = "2018",
abstract = "Purpose: Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell carcinomas and their respective margins, to characterize these cells using specific markers, to assess their self-renewal potential and determine their chemoresistance. Methods: Cell cultures were obtained from 12 tissue specimens (6 tumours and 6 margins). Total RNA was extracted and gene expression analysis was done by real-time PCR (RTPCR). Flow cytometry, immunocytometry, sphere formation and MTT assays were also applied. Results: With minor differences, cells originating from both tumours and tumour margins showed the presence of stem cell markers CD133, Nanog, Sox2, CD44, and Oct4, had the capacity to form spheroids and showed chemoresistance. Conclusions: Subpopulations of margin cells appeared to have sternness properties which might raise the question of re-evaluation of optimal surgical management.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma",
volume = "23",
number = "6",
pages = "1686-1692",
url = "https://hdl.handle.net/21.15107/rcub_smile_2271"
}

Lazarević, M., Milošević, M., Trišić, D., Toljić, B., Simonović, J., Nikolić, N., Miković, N., Jelovac, D., Petrović, M., Vukadinović, M.,& Milašin, J.. (2018). Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(6), 1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271

Lazarević M, Milošević M, Trišić D, Toljić B, Simonović J, Nikolić N, Miković N, Jelovac D, Petrović M, Vukadinović M, Milašin J. Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma. in Journal of BUON. 2018;23(6):1686-1692.
https://hdl.handle.net/21.15107/rcub_smile_2271 .

Lazarević, Miloš, Milošević, Maja, Trišić, Dijana, Toljić, Boško, Simonović, Jelena, Nikolić, Nadja, Miković, Nikola, Jelovac, Drago, Petrović, Milan, Vukadinović, Miroslav, Milašin, Jelena, "Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma" in Journal of BUON, 23, no. 6 (2018):1686-1692,
https://hdl.handle.net/21.15107/rcub_smile_2271 .

TY  - JOUR
AU  - Milošević, Maja
AU  - Lazarević, Miloš
AU  - Toljić, Boško
AU  - Simonović, Jelena
AU  - Trišić, Dijana
AU  - Nikolić, Nadja
AU  - Petrović, Milan
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2279
AB  - BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.
PB  - Wiley, Hoboken
T2  - Experimental Dermatology
T1  - Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins
VL  - 27
IS  - 10
SP  - 1160
EP  - 1165
DO  - 10.1111/exd.13755
ER  - 

@article{
author = "Milošević, Maja and Lazarević, Miloš and Toljić, Boško and Simonović, Jelena and Trišić, Dijana and Nikolić, Nadja and Petrović, Milan and Milašin, Jelena",
year = "2018",
abstract = "BackgroundUnderstanding the pathogenesis of basal cell carcinoma (BCC) and identifying the cells responsible for propagation and recurrence are crucial for the development of new treatment strategies. The aim of this study was to characterize the cells isolated from BCC and its margin. MethodsPrimary cultures were established from 10 BCCs, their respective close resection margins (3mm) and 10 control tissues. Stem cell markers analysis was carried out by real-time PCR and/or flow cytometry. Spheroid formation and MTT assays were also performed. ResultsReal-time PCR showed a higher expression of embryonic (Oct4, Sox2 and Nanog) and mesenchymal (CD44 and CD73) stem cell markers in tumors compared to margins and controls (P lt 0.05). Bmi-1 and GPR49 were also upregulated in tumors in comparison with margins. Both tumor and margin cells, but not normal, had the capacity to form spheroids. During passages, the number of spheres increased, while the diameter decreased. Tumor cells showed higher chemo-resistance compared to margin and control cells. ConclusionsBasal cell carcinomas expressed stem cell markers, pointing to the existence of a cancer cell side population with stemness characteristics. Margin also appeared to harbour a small number of cancer-initiating cells.",
publisher = "Wiley, Hoboken",
journal = "Experimental Dermatology",
title = "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins",
volume = "27",
number = "10",
pages = "1160-1165",
doi = "10.1111/exd.13755"
}

Milošević, M., Lazarević, M., Toljić, B., Simonović, J., Trišić, D., Nikolić, N., Petrović, M.,& Milašin, J.. (2018). Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology
Wiley, Hoboken., 27(10), 1160-1165.
https://doi.org/10.1111/exd.13755

Milošević M, Lazarević M, Toljić B, Simonović J, Trišić D, Nikolić N, Petrović M, Milašin J. Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins. in Experimental Dermatology. 2018;27(10):1160-1165.
doi:10.1111/exd.13755 .

Milošević, Maja, Lazarević, Miloš, Toljić, Boško, Simonović, Jelena, Trišić, Dijana, Nikolić, Nadja, Petrović, Milan, Milašin, Jelena, "Characterization of stem-like cancer cells in basal cell carcinoma and its surgical margins" in Experimental Dermatology, 27, no. 10 (2018):1160-1165,
https://doi.org/10.1111/exd.13755 . .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Jokanović, Vukoman
AU  - Antonijević, Đorđe
AU  - Marković, Dejan
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2369
AB  - Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells' niche from in vivo conditions. In an attempt to provide biomimetic microenvironments for stem cells in vitro growth, three-dimensional culture techniques have been developed. In our study advantages of newly developed porous scaffolds as the most promising in vitro imitation of niche that provides physical support, enables cell growth, regeneration and neovascularization, while they are replaced in time with newly created tissue was explained. Furthermore, dynamic cultivation techniques have been described, as new way of cell culturing that will be the main subject of our future research. In that manner, by developing an optimal dynamic culturing method, high-quality new cells and tissues would be possible to obtain, for any future clinical application.
AB  - Matične ćelije su pokazale veliki potencijal za primenu u tkivnom inženjerstvu u in vitro uslovima, regenerativnoj medicini, lečenju matičnim ćelijama i farmaceutskoj primeni. Sve ove grane, posebno primena u kliničkim istraživanjima, zahtevaće kontrolisano stvaranje visokokvalitetnih ćelija. Tradicionalnim tehnikama izolovana je većina primarnih ćelijskih kultura i ćelijskih linija, i vremenom su one postale standardne tehnike za različite ćelijske analize. Ipak, ovi uslovi gajenja nisu u mogućnosti da imitiraju dinamične, trodimenzionalne uslove mikrosredine niše matičnih ćelija iz in vivo uslova. U pokušaju da se obezbede navedeni uslovi i u in vitro uzgajanju ćelija, razvile su se trodimenzionalne tehnike gajenja ćelija. U ovom preglednom radu opisujemo prednosti novorazvijenih poroznih nosača ćelija, kao ključnih činilaca u imitaciji ćelijske niše koji obezbeđuju mehaničku potporu, omogućuju rast ćelija, regeneraciju i razvoj novih krvnih sudova, dok vremenom bivaju razgrađeni i zamenjeni novostvorenim tkivom. Dalje, tehnike dinamičnog gajenja ćelija su opisane kao vid novog načina gajenja ćelijskih kultura koji predstavlja i pravac naših budućih istraživanja. U tom smislu, razvijajući optimalan, dinamički model gajenja ćelija, biće moguće obezbediti nove ćelije i tkiva visokog kvaliteta za sva dalja klinička istraživanja.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Stem cells in tissue engineering: Dynamic cultivation requirement
T1  - Matične ćelije u tkivnom inženjerstvu - potreba za dinamičnom kultivacijom
VL  - 65
IS  - 1
SP  - 37
EP  - 44
DO  - 10.2478/sdj-2018-0005
ER  - 

@article{
author = "Trišić, Dijana and Jokanović, Vukoman and Antonijević, Đorđe and Marković, Dejan",
year = "2018",
abstract = "Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells' niche from in vivo conditions. In an attempt to provide biomimetic microenvironments for stem cells in vitro growth, three-dimensional culture techniques have been developed. In our study advantages of newly developed porous scaffolds as the most promising in vitro imitation of niche that provides physical support, enables cell growth, regeneration and neovascularization, while they are replaced in time with newly created tissue was explained. Furthermore, dynamic cultivation techniques have been described, as new way of cell culturing that will be the main subject of our future research. In that manner, by developing an optimal dynamic culturing method, high-quality new cells and tissues would be possible to obtain, for any future clinical application., Matične ćelije su pokazale veliki potencijal za primenu u tkivnom inženjerstvu u in vitro uslovima, regenerativnoj medicini, lečenju matičnim ćelijama i farmaceutskoj primeni. Sve ove grane, posebno primena u kliničkim istraživanjima, zahtevaće kontrolisano stvaranje visokokvalitetnih ćelija. Tradicionalnim tehnikama izolovana je većina primarnih ćelijskih kultura i ćelijskih linija, i vremenom su one postale standardne tehnike za različite ćelijske analize. Ipak, ovi uslovi gajenja nisu u mogućnosti da imitiraju dinamične, trodimenzionalne uslove mikrosredine niše matičnih ćelija iz in vivo uslova. U pokušaju da se obezbede navedeni uslovi i u in vitro uzgajanju ćelija, razvile su se trodimenzionalne tehnike gajenja ćelija. U ovom preglednom radu opisujemo prednosti novorazvijenih poroznih nosača ćelija, kao ključnih činilaca u imitaciji ćelijske niše koji obezbeđuju mehaničku potporu, omogućuju rast ćelija, regeneraciju i razvoj novih krvnih sudova, dok vremenom bivaju razgrađeni i zamenjeni novostvorenim tkivom. Dalje, tehnike dinamičnog gajenja ćelija su opisane kao vid novog načina gajenja ćelijskih kultura koji predstavlja i pravac naših budućih istraživanja. U tom smislu, razvijajući optimalan, dinamički model gajenja ćelija, biće moguće obezbediti nove ćelije i tkiva visokog kvaliteta za sva dalja klinička istraživanja.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Stem cells in tissue engineering: Dynamic cultivation requirement, Matične ćelije u tkivnom inženjerstvu - potreba za dinamičnom kultivacijom",
volume = "65",
number = "1",
pages = "37-44",
doi = "10.2478/sdj-2018-0005"
}

Trišić, D., Jokanović, V., Antonijević, Đ.,& Marković, D.. (2018). Stem cells in tissue engineering: Dynamic cultivation requirement. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(1), 37-44.
https://doi.org/10.2478/sdj-2018-0005

Trišić D, Jokanović V, Antonijević Đ, Marković D. Stem cells in tissue engineering: Dynamic cultivation requirement. in Stomatološki glasnik Srbije. 2018;65(1):37-44.
doi:10.2478/sdj-2018-0005 .

Trišić, Dijana, Jokanović, Vukoman, Antonijević, Đorđe, Marković, Dejan, "Stem cells in tissue engineering: Dynamic cultivation requirement" in Stomatološki glasnik Srbije, 65, no. 1 (2018):37-44,
https://doi.org/10.2478/sdj-2018-0005 . .

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Jovanović, Igor
AU  - Gjorgievska, Elizabeta
AU  - Popović, Branka
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2248
AB  - Background/Aim: The aim of this in vitro study was to investigate the influence of irradiation cycles and resting periods, on thermal effects on the external root surface during root canal irradiation of two diode laser systems (940 nm and 975 nm), at output powers of 1 W and 2 W in continuous mode. In previous studies the rising of temperature above 7°C has been reported as biologically accepted to avoid periodontal damage on the external root surface. Material and Methods: Twenty human inferior incisors were randomly distributed into four groups, the 940 nm, and the 975 nm diode laser irradiation, both with an output power of 1 W and 2 W, in continuous mode. The thermographic camera was used to detect temperature variations on the external root surface. Digital radiography of the samples was made. Results: After three cycles of irradiation, at apical third of the root, mean temperature variation by 940 nm diode laser irradiation was 2.88°C for output power of 1 W, and 6.52°C for output power of 2 W. The 975 nm laser caused a higher temperature increase in the apical region, with temperature variation of 13.56°C by an output power of 1 W, and 30.60°C at 2 W, with a statistical significance of p ≤ 0.0001 between two laser systems compared for the same power. The resting periods of 20 s between cycles were enough to lower temperature under 7°C in the case of 1 W and 2 W for 940 nm diode laser, while for 975 nm laser, after three irradiation cycles overheating occurred at both output power rates. Conclusion: Three cycles irradiation of 940 nm diode laser, with resting periods of 20 seconds, allowed safe usage of 1 W and 2 W in CW for endodontic treatment. For 975 nm at a power rate of 1 W, the last resting period drop the temperature near the safe limit and it came under 7°C in a period less than a minute, while at the power of 2 W the resting periods were not long enough for the safe temperature decrease.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Diode laser irradiation in endodontic therapy through cycles: In vitro study
VL  - 21
IS  - 2
SP  - 108
EP  - 111
DO  - 10.1515/bjdm-2017-0016
ER  - 

@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Jovanović, Igor and Gjorgievska, Elizabeta and Popović, Branka and Marković, Dejan",
year = "2017",
abstract = "Background/Aim: The aim of this in vitro study was to investigate the influence of irradiation cycles and resting periods, on thermal effects on the external root surface during root canal irradiation of two diode laser systems (940 nm and 975 nm), at output powers of 1 W and 2 W in continuous mode. In previous studies the rising of temperature above 7°C has been reported as biologically accepted to avoid periodontal damage on the external root surface. Material and Methods: Twenty human inferior incisors were randomly distributed into four groups, the 940 nm, and the 975 nm diode laser irradiation, both with an output power of 1 W and 2 W, in continuous mode. The thermographic camera was used to detect temperature variations on the external root surface. Digital radiography of the samples was made. Results: After three cycles of irradiation, at apical third of the root, mean temperature variation by 940 nm diode laser irradiation was 2.88°C for output power of 1 W, and 6.52°C for output power of 2 W. The 975 nm laser caused a higher temperature increase in the apical region, with temperature variation of 13.56°C by an output power of 1 W, and 30.60°C at 2 W, with a statistical significance of p ≤ 0.0001 between two laser systems compared for the same power. The resting periods of 20 s between cycles were enough to lower temperature under 7°C in the case of 1 W and 2 W for 940 nm diode laser, while for 975 nm laser, after three irradiation cycles overheating occurred at both output power rates. Conclusion: Three cycles irradiation of 940 nm diode laser, with resting periods of 20 seconds, allowed safe usage of 1 W and 2 W in CW for endodontic treatment. For 975 nm at a power rate of 1 W, the last resting period drop the temperature near the safe limit and it came under 7°C in a period less than a minute, while at the power of 2 W the resting periods were not long enough for the safe temperature decrease.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Diode laser irradiation in endodontic therapy through cycles: In vitro study",
volume = "21",
number = "2",
pages = "108-111",
doi = "10.1515/bjdm-2017-0016"
}

Trišić, D., Ćetenović, B., Jovanović, I., Gjorgievska, E., Popović, B.,& Marković, D.. (2017). Diode laser irradiation in endodontic therapy through cycles: In vitro study. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 21(2), 108-111.
https://doi.org/10.1515/bjdm-2017-0016

Trišić D, Ćetenović B, Jovanović I, Gjorgievska E, Popović B, Marković D. Diode laser irradiation in endodontic therapy through cycles: In vitro study. in Balkan Journal of Dental Medicine. 2017;21(2):108-111.
doi:10.1515/bjdm-2017-0016 .

Trišić, Dijana, Ćetenović, Bojana, Jovanović, Igor, Gjorgievska, Elizabeta, Popović, Branka, Marković, Dejan, "Diode laser irradiation in endodontic therapy through cycles: In vitro study" in Balkan Journal of Dental Medicine, 21, no. 2 (2017):108-111,
https://doi.org/10.1515/bjdm-2017-0016 . .

TY  - JOUR
AU  - Marković, Dejan
AU  - Rakašević, Dragana
AU  - Trišić, Dijana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2008
AB  - Lasers have found important role in clinical application, science and scientific research. The aim of this review is to focus on using soft tissue laser in endodontic treatments. The main goal of endodontic treatment is elimination of pathogenic microorganisms from root canal system. Laser light has the ability to reach parts of the tissue and areas where classical techniques and instruments cannot. New approaches to disinfecting root canals have been proposed recently, which include the use of high-power diode lasers, as well as disinfection of the root canal by using photodynamic therapy. A research is necessary to define a precise protocol for high-power laser and photodynamic therapy in treatment of the root canal system.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Application of high-power diode laser and photodynamic therapy in endodontic treatment: Review of the literature
VL  - 19
IS  - 2
SP  - 71
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_smile_2008
ER  - 

@article{
author = "Marković, Dejan and Rakašević, Dragana and Trišić, Dijana",
year = "2015",
abstract = "Lasers have found important role in clinical application, science and scientific research. The aim of this review is to focus on using soft tissue laser in endodontic treatments. The main goal of endodontic treatment is elimination of pathogenic microorganisms from root canal system. Laser light has the ability to reach parts of the tissue and areas where classical techniques and instruments cannot. New approaches to disinfecting root canals have been proposed recently, which include the use of high-power diode lasers, as well as disinfection of the root canal by using photodynamic therapy. A research is necessary to define a precise protocol for high-power laser and photodynamic therapy in treatment of the root canal system.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Application of high-power diode laser and photodynamic therapy in endodontic treatment: Review of the literature",
volume = "19",
number = "2",
pages = "71-74",
url = "https://hdl.handle.net/21.15107/rcub_smile_2008"
}

Marković, D., Rakašević, D.,& Trišić, D.. (2015). Application of high-power diode laser and photodynamic therapy in endodontic treatment: Review of the literature. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 19(2), 71-74.
https://hdl.handle.net/21.15107/rcub_smile_2008

Marković D, Rakašević D, Trišić D. Application of high-power diode laser and photodynamic therapy in endodontic treatment: Review of the literature. in Balkan Journal of Dental Medicine. 2015;19(2):71-74.
https://hdl.handle.net/21.15107/rcub_smile_2008 .

Marković, Dejan, Rakašević, Dragana, Trišić, Dijana, "Application of high-power diode laser and photodynamic therapy in endodontic treatment: Review of the literature" in Balkan Journal of Dental Medicine, 19, no. 2 (2015):71-74,
https://hdl.handle.net/21.15107/rcub_smile_2008 .