TY  - JOUR
AU  - Nikolić-Jakoba, Nataša
AU  - Vojnović, Sandra
AU  - Pavić, A.
AU  - Janković, Saša
AU  - Leković, Vojislav
AU  - Vasiljević, Branka
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1696
AB  - Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?
VL  - 64
IS  - 4
SP  - 1413
EP  - 1423
DO  - 10.2298/ABS1204413J
ER  - 

@article{
author = "Nikolić-Jakoba, Nataša and Vojnović, Sandra and Pavić, A. and Janković, Saša and Leković, Vojislav and Vasiljević, Branka",
year = "2012",
abstract = "Aggregatibacter actinomycetemcomitans is considered one of the bacterial species of etiological importance in periodontitis. The aim of this study was to evaluate the serotype of A. actinomycetemcomitans in the subgingival biofilm in subjects with periodontal health and disease. Pooled samples of subgingival plaque were taken for culture-based identification of microorganisms. Colonies suspected to be A. actinomycetemcomitans were selected for molecular identification using either multiplex or conventional PCR in serotype-specific genotyping and 16S rRNA gene sequencing. In silico analysis showed that most selected colonies belong to the genus Campylobacter, although positive signals for serotypes of A. actinomycetemcomitans were obtained with these samples. Identification of A. actinomycetemcomitans by conventional PCR for 16S rRNA with one species-specific and one universal primer was inconclusive because an almost identical signal with Campylobacter gracilis was obtained. Although PCR-based methods for the identification of A. actinomycetemcomitans are more rapid, sequencing should not be omitted.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?",
volume = "64",
number = "4",
pages = "1413-1423",
doi = "10.2298/ABS1204413J"
}

Nikolić-Jakoba, N., Vojnović, S., Pavić, A., Janković, S., Leković, V.,& Vasiljević, B.. (2012). Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1413-1423.
https://doi.org/10.2298/ABS1204413J

Nikolić-Jakoba N, Vojnović S, Pavić A, Janković S, Leković V, Vasiljević B. Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?. in Archives of Biological Sciences. 2012;64(4):1413-1423.
doi:10.2298/ABS1204413J .

Nikolić-Jakoba, Nataša, Vojnović, Sandra, Pavić, A., Janković, Saša, Leković, Vojislav, Vasiljević, Branka, "Polymerase chain reaction in the identification of periodontopathogens: A reliable and satisfactory method?" in Archives of Biological Sciences, 64, no. 4 (2012):1413-1423,
https://doi.org/10.2298/ABS1204413J . .