TY  - JOUR
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Pekmezović, Marina
AU  - Marinković, Jelena
AU  - Živković, Rade
AU  - Arsić-Arsenijević, Valentina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2379
AB  - Purpose Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to DS, but it seems that Candida plays the key role. The aim of the study was to evaluate the denture sonication method to: (i) increase the possibility of diagnosing patients with Candida-associated DS; (ii) detect and identify the mixed Candida spp., and (iii) determine the Candida colony forming units (CFU) and its possible relationship with DS severity, based on Newton's classification. Materials and Methods The cross-sectional study conducted at the Clinic for Dental Prosthetics, Belgrade (Serbia) from June 2013 to December 2014 enrolled edentulous patients with dentures (n = 250). Patients without DS (n = 20) were the control group (CG). The patients' data were collected, and patients with DS (study group/SG) were selected and divided into SG Candida+ and SG Candida-. Based on severity of DS, the SG patients were classified in 3 groups (Newton's classification). Four sampling methods were applied to detect patients with Candida-associated DS: mucosa swab, denture swab, oral rinse, and denture sonication method. The sensitivity and specificity of denture sonication method were shown using the receiver operating characteristic (ROC) curve and the area under the curve (AUC). Results In 97 (38.8%), out of 250 clinically examined patients, DS was diagnosed. In 82 (84.5%), out of 97 mycologically examined patients, Candida-associated DS was detected when denture sonication method was applied. Additionally, using the denture sonication method we observed: (i) the largest number of Candida positive patients compared to other sampling methods (p  lt  0.0001); (ii) the highest number of Candida CFU/ml (10(5)), and (iii) the possibility to detect mixed Candida cultures. The largest number of patients with Candida-associated DS showed type II (60%) DS, followed by type I (21%), and type III (19%) DS. Conclusion The denture sonication method is easy, accurate, and sensitive, and increases the possibility of diagnosing patients with Candida-associated DS. Additionally, yeast quantification, mixed Candida spp., and non-albicans Candida were detectable when cultivation on Candida CHROMagar was performed. It was not possible using conventional methods, such as swab or oral rinse.
PB  - Wiley, Hoboken
T2  - Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
T1  - Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis
VL  - 28
IS  - 5
SP  - 580
EP  - 586
DO  - 10.1111/jopr.12610
ER  - 

@article{
author = "Perić, Mirjana and Radunović, Milena and Pekmezović, Marina and Marinković, Jelena and Živković, Rade and Arsić-Arsenijević, Valentina",
year = "2019",
abstract = "Purpose Denture stomatitis (DS) is a disease characterized by inflammation and erythema of the oral mucosa areas covered by the denture. Multifactorial etiological factors contribute to DS, but it seems that Candida plays the key role. The aim of the study was to evaluate the denture sonication method to: (i) increase the possibility of diagnosing patients with Candida-associated DS; (ii) detect and identify the mixed Candida spp., and (iii) determine the Candida colony forming units (CFU) and its possible relationship with DS severity, based on Newton's classification. Materials and Methods The cross-sectional study conducted at the Clinic for Dental Prosthetics, Belgrade (Serbia) from June 2013 to December 2014 enrolled edentulous patients with dentures (n = 250). Patients without DS (n = 20) were the control group (CG). The patients' data were collected, and patients with DS (study group/SG) were selected and divided into SG Candida+ and SG Candida-. Based on severity of DS, the SG patients were classified in 3 groups (Newton's classification). Four sampling methods were applied to detect patients with Candida-associated DS: mucosa swab, denture swab, oral rinse, and denture sonication method. The sensitivity and specificity of denture sonication method were shown using the receiver operating characteristic (ROC) curve and the area under the curve (AUC). Results In 97 (38.8%), out of 250 clinically examined patients, DS was diagnosed. In 82 (84.5%), out of 97 mycologically examined patients, Candida-associated DS was detected when denture sonication method was applied. Additionally, using the denture sonication method we observed: (i) the largest number of Candida positive patients compared to other sampling methods (p  lt  0.0001); (ii) the highest number of Candida CFU/ml (10(5)), and (iii) the possibility to detect mixed Candida cultures. The largest number of patients with Candida-associated DS showed type II (60%) DS, followed by type I (21%), and type III (19%) DS. Conclusion The denture sonication method is easy, accurate, and sensitive, and increases the possibility of diagnosing patients with Candida-associated DS. Additionally, yeast quantification, mixed Candida spp., and non-albicans Candida were detectable when cultivation on Candida CHROMagar was performed. It was not possible using conventional methods, such as swab or oral rinse.",
publisher = "Wiley, Hoboken",
journal = "Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry",
title = "Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis",
volume = "28",
number = "5",
pages = "580-586",
doi = "10.1111/jopr.12610"
}

Perić, M., Radunović, M., Pekmezović, M., Marinković, J., Živković, R.,& Arsić-Arsenijević, V.. (2019). Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry
Wiley, Hoboken., 28(5), 580-586.
https://doi.org/10.1111/jopr.12610

Perić M, Radunović M, Pekmezović M, Marinković J, Živković R, Arsić-Arsenijević V. Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis. in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry. 2019;28(5):580-586.
doi:10.1111/jopr.12610 .

Perić, Mirjana, Radunović, Milena, Pekmezović, Marina, Marinković, Jelena, Živković, Rade, Arsić-Arsenijević, Valentina, "Laboratory-Based Investigation of Denture Sonication Method in Patients with Candida-Associated Denture Stomatitis" in Journal of Prosthodontics-Implant Esthetic & Reconstructive Dentistry, 28, no. 5 (2019):580-586,
https://doi.org/10.1111/jopr.12610 . .

TY  - JOUR
AU  - Barac, Aleksandra
AU  - Stevanović, Goran
AU  - Pekmezović, Marina
AU  - Rakočević, Zoran
AU  - Stošović, Rajica
AU  - Erović, Boban
AU  - Tomić-Spirić, Vesna
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2310
AB  - Dysbiosis of the microbiome on the airway mucosa leads to the development of chronic inflammatory and allergic disorders. The aim of this study was to consider the potential diagnostic criteria for allergic fungal rhinosinusitis (AFRS) and nonallergic fungal rhinosinusitis (FRS), and the role of fungal presence in an environment for the development of AFRS. In this study, 136 patients were divided into two groups: patients with positive specific immunoglobulin E (sIgE) and fungal finding (AFRS group), and patients with negative sIgE and positive fungal finding (FRS group). The study design included: anamnesis data, sIgE, eosinophil count and skin-prick test, rhinology and computerized tomography (CT) observation and mycological finding. Our results showed: (i) the prevalence in Serbia is: AFRS 1.3%, FRS 2.8%; (ii) 30.4% patients with sIgE+ had more often severe and recurrent chronic rhinosinusitis (CRS) (P = .005) and the presence of polyps (P = .025); (iii) 46.4% patients with sIgE+ had positive fungi on the sinonasal mucosa and were considered as AFRS; (iv) patients with AFRS had more frequent asthma (P = .024) and chronicity of CRS > 10 years (P = .000). The persistent fungal presence and prolonged duration of CRS could be a silent threat for the progression of inflammation and development of FRS. Lavage with hypertonic-NaCl should be included in the everyday hygiene routine in an effort to decrease fungal load and antigenic exposure. The presence of allergological parameters and better response to corticosteroid therapy in AFRS patients should be considered as crucial diagnostic criteria for AFRS.
PB  - Oxford Univ Press, Oxford
T2  - Medical Mycology
T1  - Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis
VL  - 56
IS  - 2
SP  - 162
EP  - 171
DO  - 10.1093/mmy/myx032
ER  - 

@article{
author = "Barac, Aleksandra and Stevanović, Goran and Pekmezović, Marina and Rakočević, Zoran and Stošović, Rajica and Erović, Boban and Tomić-Spirić, Vesna",
year = "2018",
abstract = "Dysbiosis of the microbiome on the airway mucosa leads to the development of chronic inflammatory and allergic disorders. The aim of this study was to consider the potential diagnostic criteria for allergic fungal rhinosinusitis (AFRS) and nonallergic fungal rhinosinusitis (FRS), and the role of fungal presence in an environment for the development of AFRS. In this study, 136 patients were divided into two groups: patients with positive specific immunoglobulin E (sIgE) and fungal finding (AFRS group), and patients with negative sIgE and positive fungal finding (FRS group). The study design included: anamnesis data, sIgE, eosinophil count and skin-prick test, rhinology and computerized tomography (CT) observation and mycological finding. Our results showed: (i) the prevalence in Serbia is: AFRS 1.3%, FRS 2.8%; (ii) 30.4% patients with sIgE+ had more often severe and recurrent chronic rhinosinusitis (CRS) (P = .005) and the presence of polyps (P = .025); (iii) 46.4% patients with sIgE+ had positive fungi on the sinonasal mucosa and were considered as AFRS; (iv) patients with AFRS had more frequent asthma (P = .024) and chronicity of CRS > 10 years (P = .000). The persistent fungal presence and prolonged duration of CRS could be a silent threat for the progression of inflammation and development of FRS. Lavage with hypertonic-NaCl should be included in the everyday hygiene routine in an effort to decrease fungal load and antigenic exposure. The presence of allergological parameters and better response to corticosteroid therapy in AFRS patients should be considered as crucial diagnostic criteria for AFRS.",
publisher = "Oxford Univ Press, Oxford",
journal = "Medical Mycology",
title = "Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis",
volume = "56",
number = "2",
pages = "162-171",
doi = "10.1093/mmy/myx032"
}

Barac, A., Stevanović, G., Pekmezović, M., Rakočević, Z., Stošović, R., Erović, B.,& Tomić-Spirić, V.. (2018). Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis. in Medical Mycology
Oxford Univ Press, Oxford., 56(2), 162-171.
https://doi.org/10.1093/mmy/myx032

Barac A, Stevanović G, Pekmezović M, Rakočević Z, Stošović R, Erović B, Tomić-Spirić V. Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis. in Medical Mycology. 2018;56(2):162-171.
doi:10.1093/mmy/myx032 .

Barac, Aleksandra, Stevanović, Goran, Pekmezović, Marina, Rakočević, Zoran, Stošović, Rajica, Erović, Boban, Tomić-Spirić, Vesna, "Study toward resolving the controversy over the definition of allergic fungal rhinosinusitis" in Medical Mycology, 56, no. 2 (2018):162-171,
https://doi.org/10.1093/mmy/myx032 . .

TY  - CONF
AU  - Barac, Aleksandra
AU  - Pekmezović, Marina
AU  - Rakočević, Zoran
AU  - Janović, Aleksa
AU  - Bracanović, Đurđa
AU  - Antić, Svetlana
AU  - Stevanović, G.
AU  - Tomić-Spirić, Vesna
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2221
PB  - Wiley, Hoboken
C3  - Allergy
T1  - Are there any specific signs of allergic fungal rhinosinusitis visible by computed tomography scan?
VL  - 72
SP  - 206
EP  - 206
UR  - https://hdl.handle.net/21.15107/rcub_smile_2221
ER  - 

@conference{
author = "Barac, Aleksandra and Pekmezović, Marina and Rakočević, Zoran and Janović, Aleksa and Bracanović, Đurđa and Antić, Svetlana and Stevanović, G. and Tomić-Spirić, Vesna",
year = "2017",
publisher = "Wiley, Hoboken",
journal = "Allergy",
title = "Are there any specific signs of allergic fungal rhinosinusitis visible by computed tomography scan?",
volume = "72",
pages = "206-206",
url = "https://hdl.handle.net/21.15107/rcub_smile_2221"
}

Barac, A., Pekmezović, M., Rakočević, Z., Janović, A., Bracanović, Đ., Antić, S., Stevanović, G.,& Tomić-Spirić, V.. (2017). Are there any specific signs of allergic fungal rhinosinusitis visible by computed tomography scan?. in Allergy
Wiley, Hoboken., 72, 206-206.
https://hdl.handle.net/21.15107/rcub_smile_2221

Barac A, Pekmezović M, Rakočević Z, Janović A, Bracanović Đ, Antić S, Stevanović G, Tomić-Spirić V. Are there any specific signs of allergic fungal rhinosinusitis visible by computed tomography scan?. in Allergy. 2017;72:206-206.
https://hdl.handle.net/21.15107/rcub_smile_2221 .

Barac, Aleksandra, Pekmezović, Marina, Rakočević, Zoran, Janović, Aleksa, Bracanović, Đurđa, Antić, Svetlana, Stevanović, G., Tomić-Spirić, Vesna, "Are there any specific signs of allergic fungal rhinosinusitis visible by computed tomography scan?" in Allergy, 72 (2017):206-206,
https://hdl.handle.net/21.15107/rcub_smile_2221 .

TY  - CONF
AU  - Barac, Aleksandra
AU  - Pekmezović, Marina
AU  - Rajković, Katarina
AU  - Rakočević, Zoran
AU  - Janović, Aleksa
AU  - Bracanović, Đurđa
AU  - Stošović, Rajica
AU  - Spirić, Tomić, V
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2085
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - Mathematical modeling and image analysis: possible clinical application in practice as a predictor of fungal rhinosinusitis
VL  - 71
SP  - 426
EP  - 426
UR  - https://hdl.handle.net/21.15107/rcub_smile_2085
ER  - 

@conference{
author = "Barac, Aleksandra and Pekmezović, Marina and Rajković, Katarina and Rakočević, Zoran and Janović, Aleksa and Bracanović, Đurđa and Stošović, Rajica and Spirić, Tomić, V",
year = "2016",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "Mathematical modeling and image analysis: possible clinical application in practice as a predictor of fungal rhinosinusitis",
volume = "71",
pages = "426-426",
url = "https://hdl.handle.net/21.15107/rcub_smile_2085"
}

Barac, A., Pekmezović, M., Rajković, K., Rakočević, Z., Janović, A., Bracanović, Đ., Stošović, R.,& Spirić, T. V.. (2016). Mathematical modeling and image analysis: possible clinical application in practice as a predictor of fungal rhinosinusitis. in Allergy
Wiley-Blackwell, Hoboken., 71, 426-426.
https://hdl.handle.net/21.15107/rcub_smile_2085

Barac A, Pekmezović M, Rajković K, Rakočević Z, Janović A, Bracanović Đ, Stošović R, Spirić TV. Mathematical modeling and image analysis: possible clinical application in practice as a predictor of fungal rhinosinusitis. in Allergy. 2016;71:426-426.
https://hdl.handle.net/21.15107/rcub_smile_2085 .

Barac, Aleksandra, Pekmezović, Marina, Rajković, Katarina, Rakočević, Zoran, Janović, Aleksa, Bracanović, Đurđa, Stošović, Rajica, Spirić, Tomić, V, "Mathematical modeling and image analysis: possible clinical application in practice as a predictor of fungal rhinosinusitis" in Allergy, 71 (2016):426-426,
https://hdl.handle.net/21.15107/rcub_smile_2085 .

TY  - JOUR
AU  - Barac, Aleksandra
AU  - Pekmezović, Marina
AU  - Milobratović, Danica
AU  - Otašević-Tasić, Suzana
AU  - Radunović, Milena
AU  - Arsić-Arsenijević, Valentina
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2023
AB  - Malassezia yeast belongs to the normal cutaneous flora and under certain conditions it causes seborrhoeic dermatitis (SD). There is no culture-based study about the presence and density of the Malassezia in SD patients in Serbia. Aim was to show the presence, species distribution and density of Malassezia in patients with SD on lesional skin (LS) and non-lesional skin (NLS) and healthy controls (HC) and to compare data between Serbia and other countries. The study included 70 HC and 60 patients with SD in the study group (SG). Isolation, identification and examination of density of Malassezia colony-forming units from LS and NLS were performed. Malassezia was found more frequently in the SG than in HC, 90% and 60%, respectively (P lt 0.01). The most frequent isolates in SG on LS were M. slooffiae (26%), followed by M. globosa (17%) and M. sympodialis (17%). The yeast density was much higher on LS of SG than on NLS of SG or in the HC group (P lt 0.05). Higher density of Malassezia was shown on LS of SG than on NLS of SG and HC. M. slooffiae is the most prevalent species in SD patients in Serbia. This study demonstrated a positive relationship between severity of SD and presence of Malassezia spp.
PB  - Wiley, Hoboken
T2  - Mycoses
T1  - Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature
VL  - 58
IS  - 2
SP  - 69
EP  - 75
DO  - 10.1111/myc.12276
ER  - 

@article{
author = "Barac, Aleksandra and Pekmezović, Marina and Milobratović, Danica and Otašević-Tasić, Suzana and Radunović, Milena and Arsić-Arsenijević, Valentina",
year = "2015",
abstract = "Malassezia yeast belongs to the normal cutaneous flora and under certain conditions it causes seborrhoeic dermatitis (SD). There is no culture-based study about the presence and density of the Malassezia in SD patients in Serbia. Aim was to show the presence, species distribution and density of Malassezia in patients with SD on lesional skin (LS) and non-lesional skin (NLS) and healthy controls (HC) and to compare data between Serbia and other countries. The study included 70 HC and 60 patients with SD in the study group (SG). Isolation, identification and examination of density of Malassezia colony-forming units from LS and NLS were performed. Malassezia was found more frequently in the SG than in HC, 90% and 60%, respectively (P lt 0.01). The most frequent isolates in SG on LS were M. slooffiae (26%), followed by M. globosa (17%) and M. sympodialis (17%). The yeast density was much higher on LS of SG than on NLS of SG or in the HC group (P lt 0.05). Higher density of Malassezia was shown on LS of SG than on NLS of SG and HC. M. slooffiae is the most prevalent species in SD patients in Serbia. This study demonstrated a positive relationship between severity of SD and presence of Malassezia spp.",
publisher = "Wiley, Hoboken",
journal = "Mycoses",
title = "Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature",
volume = "58",
number = "2",
pages = "69-75",
doi = "10.1111/myc.12276"
}

Barac, A., Pekmezović, M., Milobratović, D., Otašević-Tasić, S., Radunović, M.,& Arsić-Arsenijević, V.. (2015). Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature. in Mycoses
Wiley, Hoboken., 58(2), 69-75.
https://doi.org/10.1111/myc.12276

Barac A, Pekmezović M, Milobratović D, Otašević-Tasić S, Radunović M, Arsić-Arsenijević V. Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature. in Mycoses. 2015;58(2):69-75.
doi:10.1111/myc.12276 .

Barac, Aleksandra, Pekmezović, Marina, Milobratović, Danica, Otašević-Tasić, Suzana, Radunović, Milena, Arsić-Arsenijević, Valentina, "Presence, species distribution, and density of Malassezia yeast in patients with seborrhoeic dermatitis - a community-based case-control study and review of literature" in Mycoses, 58, no. 2 (2015):69-75,
https://doi.org/10.1111/myc.12276 . .

TY  - JOUR
AU  - Živković, Rade
AU  - Perić, Mirjana
AU  - Arsić-Arsenijević, Valentina
AU  - Martinović, Željko
AU  - Pekmezović, Marina
AU  - Stojić, Ž.
AU  - Raičković, Vanja
AU  - Đurišić, Slobodan
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1782
AB  - Candida spp. form a part of human and animal oral cavity flora. However Candida spp. is the main cause of dental related stomatitis in humans and stomatitis in dogs. Stomatitis treatment implies the use of azoles and polyenes to which yeasts build up resistance. The research is directed to the use of natural compounds such as essential oils. The aim of this paper is to define the antifungal activity of thyme oil on 15 clinical strains of Candida spp., isolated from humans and dogs and to determine if there is a difference in susceptibility between human and dog isolates. Sampling in patients with stomatitis was done by swabbing the denture or oral mucosa swab while sampling in dogs was done by swabbing the oral cavity mucosa after stomatitis has been diagnosed. In order to investigate the antifungal activity of thyme oil in vitro, microdilution method was used. Thyme oil expressed antifungal effects on all investigated strains. Also, our data show that the values of minimum fungicide concentration (MFC) and minimum inhibitory concentration (MIC) are lower in human strains. Explanation is that in most cases, stomatitis in humans is asymptomatic and thus not treated, so Candida strains have not developed resistance. On the other hand, stomatitis in dogs is followed by a marked clinical picture and treated is by antimicotics (mostly by azoles), therefore resistant Candida strains are more likely to occur.
AB  - Candida spp. je sastavni deo mikrobioma usne duplje čoveka i psa. Međutim Candida spp. predstavlja glavni uzročnik proteznog stomatitisa kod čoveka i stomatitisa kod psa. Terapija stomatitisa podrazumeva korišćenje azola i poliena na koje poslednjih godina gljivice razvijaju otpornost. Istrživanja se usmeravaju ka primeni prirodnih preparata kao što su etarska ulja. Cilj rada je utvrdi antifungalnu aktivnost ulja timijana na kliničke izolate Candida spp. čoveka i psa, i utvrdi da li postoji razlika u osetljivosti sojeva Candide spp. izolovanih kod čoveka i psa. Uzorkovanje kod pacijenata sa stomatitisom je vršeno brisom proteze ili brisom sluzokože. Kod pasa je uzrokovanje vršeno brisom sluzokože usne duplje kada je dijagnostikovan stomatitis. Zasejani sojevi su in vitro tretirani korišćenjem mikrodilucione metode da bi se utvrdilo da li ulje timijana deluje na kliničke izolate Candida spp. čoveka i psa. Rezultati pokazuju da su vrednosti minimalne fungicidne koncentracije (MFC) i minimalne inhibitorne koncentracije (MIC) manje kod čoveka. Stomatitis je kod ljudi najčešće je asimptomatski, pacijenti se ne podvrgavaju lečenju, pa sojevi Candida spp. nisu razvili rezistenciju na antifungicidne preparate. Kod pasa stomatitis je praćen burnijom kliničkom slikom, leči se antimikoticima (uglavnom azolima) pa je verovatnoća pojave rezistentnih sojeva Candida spp. veća.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Susceptibility profile of Candida spp. isolated from humans and dogs with stomatitis to the essential oil of Thymus vulgaris
T1  - Osetljivost sojeva Candida spp. izolovanih kod čoveka i psa sa stomatitisom na etarsko ulje timijana
VL  - 63
IS  - 5-6
SP  - 707
EP  - 715
DO  - 10.2298/AVB1306707Z
ER  - 

@article{
author = "Živković, Rade and Perić, Mirjana and Arsić-Arsenijević, Valentina and Martinović, Željko and Pekmezović, Marina and Stojić, Ž. and Raičković, Vanja and Đurišić, Slobodan",
year = "2013",
abstract = "Candida spp. form a part of human and animal oral cavity flora. However Candida spp. is the main cause of dental related stomatitis in humans and stomatitis in dogs. Stomatitis treatment implies the use of azoles and polyenes to which yeasts build up resistance. The research is directed to the use of natural compounds such as essential oils. The aim of this paper is to define the antifungal activity of thyme oil on 15 clinical strains of Candida spp., isolated from humans and dogs and to determine if there is a difference in susceptibility between human and dog isolates. Sampling in patients with stomatitis was done by swabbing the denture or oral mucosa swab while sampling in dogs was done by swabbing the oral cavity mucosa after stomatitis has been diagnosed. In order to investigate the antifungal activity of thyme oil in vitro, microdilution method was used. Thyme oil expressed antifungal effects on all investigated strains. Also, our data show that the values of minimum fungicide concentration (MFC) and minimum inhibitory concentration (MIC) are lower in human strains. Explanation is that in most cases, stomatitis in humans is asymptomatic and thus not treated, so Candida strains have not developed resistance. On the other hand, stomatitis in dogs is followed by a marked clinical picture and treated is by antimicotics (mostly by azoles), therefore resistant Candida strains are more likely to occur., Candida spp. je sastavni deo mikrobioma usne duplje čoveka i psa. Međutim Candida spp. predstavlja glavni uzročnik proteznog stomatitisa kod čoveka i stomatitisa kod psa. Terapija stomatitisa podrazumeva korišćenje azola i poliena na koje poslednjih godina gljivice razvijaju otpornost. Istrživanja se usmeravaju ka primeni prirodnih preparata kao što su etarska ulja. Cilj rada je utvrdi antifungalnu aktivnost ulja timijana na kliničke izolate Candida spp. čoveka i psa, i utvrdi da li postoji razlika u osetljivosti sojeva Candide spp. izolovanih kod čoveka i psa. Uzorkovanje kod pacijenata sa stomatitisom je vršeno brisom proteze ili brisom sluzokože. Kod pasa je uzrokovanje vršeno brisom sluzokože usne duplje kada je dijagnostikovan stomatitis. Zasejani sojevi su in vitro tretirani korišćenjem mikrodilucione metode da bi se utvrdilo da li ulje timijana deluje na kliničke izolate Candida spp. čoveka i psa. Rezultati pokazuju da su vrednosti minimalne fungicidne koncentracije (MFC) i minimalne inhibitorne koncentracije (MIC) manje kod čoveka. Stomatitis je kod ljudi najčešće je asimptomatski, pacijenti se ne podvrgavaju lečenju, pa sojevi Candida spp. nisu razvili rezistenciju na antifungicidne preparate. Kod pasa stomatitis je praćen burnijom kliničkom slikom, leči se antimikoticima (uglavnom azolima) pa je verovatnoća pojave rezistentnih sojeva Candida spp. veća.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Susceptibility profile of Candida spp. isolated from humans and dogs with stomatitis to the essential oil of Thymus vulgaris, Osetljivost sojeva Candida spp. izolovanih kod čoveka i psa sa stomatitisom na etarsko ulje timijana",
volume = "63",
number = "5-6",
pages = "707-715",
doi = "10.2298/AVB1306707Z"
}

Živković, R., Perić, M., Arsić-Arsenijević, V., Martinović, Ž., Pekmezović, M., Stojić, Ž., Raičković, V.,& Đurišić, S.. (2013). Susceptibility profile of Candida spp. isolated from humans and dogs with stomatitis to the essential oil of Thymus vulgaris. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 707-715.
https://doi.org/10.2298/AVB1306707Z

Živković R, Perić M, Arsić-Arsenijević V, Martinović Ž, Pekmezović M, Stojić Ž, Raičković V, Đurišić S. Susceptibility profile of Candida spp. isolated from humans and dogs with stomatitis to the essential oil of Thymus vulgaris. in Acta veterinaria. 2013;63(5-6):707-715.
doi:10.2298/AVB1306707Z .

Živković, Rade, Perić, Mirjana, Arsić-Arsenijević, Valentina, Martinović, Željko, Pekmezović, Marina, Stojić, Ž., Raičković, Vanja, Đurišić, Slobodan, "Susceptibility profile of Candida spp. isolated from humans and dogs with stomatitis to the essential oil of Thymus vulgaris" in Acta veterinaria, 63, no. 5-6 (2013):707-715,
https://doi.org/10.2298/AVB1306707Z . .