TY  - JOUR
AU  - Stasić, Jovana
AU  - Selaković, Nenad
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
AU  - Veljović, Đorđe
AU  - Miletić, Vesna
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2535
AB  - ObjectivesThe study aims to evaluate the effects of non-thermal atmospheric plasma (NTAP) treatments on dentin wetting and surface free energy (SFE) and compare the effects of NTAP treatment, etch-and-rinse, and self-etch protocols for application of universal adhesives.Materials and methodsMid-coronal dentin of intact third molars was used to measure contact angles of distilled water, ethylene-glycol, and diiodomethane and calculate SFE following different NTAP preset treatments (feeding gas consisting of pure He, He + 1% O-2, He + 1.5% O-2), power input (1 or 3W), and tip-to-surface distance (2, 4, or 8mm). Contact angles of reference liquids and SFE of dentin following He + 1.5% O-2 at 3-W and 4-mm treatment was compared to phosphoric acid etching. Contact angles of Single Bond Universal (SBU; 3M ESPE) and Clearfil Universal Bond (CUB; Kuraray Noritake) were measured following NTAP, etch-and-rinse, and self-etch protocols.ResultsNTAP significantly reduced contact angles of reference liquids and increased dentin SFE compared to untreated dentin (p lt 0.05). O-2 intensified the effect of He NTAP (p lt 0.05). NTAP and phosphoric acid increased dentin polarity and Lewis base surface characteristics. Phosphoric acid increased contact angles of adhesives compared to the self-etch protocol (p lt 0.05). NTAP resulted in lower adhesive contact angles than phosphoric acid, the difference being statistically significant for CUB (p lt 0.05). Compared to the self-etch protocol, NTAP slightly reduced CUB contact angle but not that of SBU (p>0.05).ConclusionsHe NTAP with and without O-2 increased dentin wetting and SFE, surpassing the effect of phosphoric acid and lowering adhesive contact angles. NTAP produced no apparent micro-morphological changes on dentin surface comparable to acid etching.Clinical significanceNTAP treatment of dentin prior to adhesive application increases dentin wetting and surface free energy facilitating better adhesive distribution on dentin surface compared to phosphoric acid etching and similar to the self-etch application protocol.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives
VL  - 23
IS  - 3
SP  - 1383
EP  - 1396
DO  - 10.1007/s00784-018-2563-2
ER  - 

@article{
author = "Stasić, Jovana and Selaković, Nenad and Puač, Nevena and Miletić, Maja and Malović, Gordana and Petrović, Zoran Lj. and Veljović, Đorđe and Miletić, Vesna",
year = "2019",
abstract = "ObjectivesThe study aims to evaluate the effects of non-thermal atmospheric plasma (NTAP) treatments on dentin wetting and surface free energy (SFE) and compare the effects of NTAP treatment, etch-and-rinse, and self-etch protocols for application of universal adhesives.Materials and methodsMid-coronal dentin of intact third molars was used to measure contact angles of distilled water, ethylene-glycol, and diiodomethane and calculate SFE following different NTAP preset treatments (feeding gas consisting of pure He, He + 1% O-2, He + 1.5% O-2), power input (1 or 3W), and tip-to-surface distance (2, 4, or 8mm). Contact angles of reference liquids and SFE of dentin following He + 1.5% O-2 at 3-W and 4-mm treatment was compared to phosphoric acid etching. Contact angles of Single Bond Universal (SBU; 3M ESPE) and Clearfil Universal Bond (CUB; Kuraray Noritake) were measured following NTAP, etch-and-rinse, and self-etch protocols.ResultsNTAP significantly reduced contact angles of reference liquids and increased dentin SFE compared to untreated dentin (p lt 0.05). O-2 intensified the effect of He NTAP (p lt 0.05). NTAP and phosphoric acid increased dentin polarity and Lewis base surface characteristics. Phosphoric acid increased contact angles of adhesives compared to the self-etch protocol (p lt 0.05). NTAP resulted in lower adhesive contact angles than phosphoric acid, the difference being statistically significant for CUB (p lt 0.05). Compared to the self-etch protocol, NTAP slightly reduced CUB contact angle but not that of SBU (p>0.05).ConclusionsHe NTAP with and without O-2 increased dentin wetting and SFE, surpassing the effect of phosphoric acid and lowering adhesive contact angles. NTAP produced no apparent micro-morphological changes on dentin surface comparable to acid etching.Clinical significanceNTAP treatment of dentin prior to adhesive application increases dentin wetting and surface free energy facilitating better adhesive distribution on dentin surface compared to phosphoric acid etching and similar to the self-etch application protocol.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives",
volume = "23",
number = "3",
pages = "1383-1396",
doi = "10.1007/s00784-018-2563-2"
}

Stasić, J., Selaković, N., Puač, N., Miletić, M., Malović, G., Petrović, Z. Lj., Veljović, Đ.,& Miletić, V.. (2019). Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(3), 1383-1396.
https://doi.org/10.1007/s00784-018-2563-2

Stasić J, Selaković N, Puač N, Miletić M, Malović G, Petrović ZL, Veljović Đ, Miletić V. Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives. in Clinical Oral Investigations. 2019;23(3):1383-1396.
doi:10.1007/s00784-018-2563-2 .

Stasić, Jovana, Selaković, Nenad, Puač, Nevena, Miletić, Maja, Malović, Gordana, Petrović, Zoran Lj., Veljović, Đorđe, Miletić, Vesna, "Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives" in Clinical Oral Investigations, 23, no. 3 (2019):1383-1396,
https://doi.org/10.1007/s00784-018-2563-2 . .

TY  - JOUR
AU  - Stasić, Jovana
AU  - Selaković, Nenad
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
AU  - Veljović, Đorđe
AU  - Miletić, Vesna
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2389
AB  - ObjectivesThe study aims to evaluate the effects of non-thermal atmospheric plasma (NTAP) treatments on dentin wetting and surface free energy (SFE) and compare the effects of NTAP treatment, etch-and-rinse, and self-etch protocols for application of universal adhesives.Materials and methodsMid-coronal dentin of intact third molars was used to measure contact angles of distilled water, ethylene-glycol, and diiodomethane and calculate SFE following different NTAP preset treatments (feeding gas consisting of pure He, He + 1% O-2, He + 1.5% O-2), power input (1 or 3W), and tip-to-surface distance (2, 4, or 8mm). Contact angles of reference liquids and SFE of dentin following He + 1.5% O-2 at 3-W and 4-mm treatment was compared to phosphoric acid etching. Contact angles of Single Bond Universal (SBU; 3M ESPE) and Clearfil Universal Bond (CUB; Kuraray Noritake) were measured following NTAP, etch-and-rinse, and self-etch protocols.ResultsNTAP significantly reduced contact angles of reference liquids and increased dentin SFE compared to untreated dentin (p lt 0.05). O-2 intensified the effect of He NTAP (p lt 0.05). NTAP and phosphoric acid increased dentin polarity and Lewis base surface characteristics. Phosphoric acid increased contact angles of adhesives compared to the self-etch protocol (p lt 0.05). NTAP resulted in lower adhesive contact angles than phosphoric acid, the difference being statistically significant for CUB (p lt 0.05). Compared to the self-etch protocol, NTAP slightly reduced CUB contact angle but not that of SBU (p>0.05).ConclusionsHe NTAP with and without O-2 increased dentin wetting and SFE, surpassing the effect of phosphoric acid and lowering adhesive contact angles. NTAP produced no apparent micro-morphological changes on dentin surface comparable to acid etching.Clinical significanceNTAP treatment of dentin prior to adhesive application increases dentin wetting and surface free energy facilitating better adhesive distribution on dentin surface compared to phosphoric acid etching and similar to the self-etch application protocol.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives
VL  - 23
IS  - 3
SP  - 1383
EP  - 1396
DO  - 10.1007/s00784-018-2563-2
ER  - 

@article{
author = "Stasić, Jovana and Selaković, Nenad and Puač, Nevena and Miletić, Maja and Malović, Gordana and Petrović, Zoran Lj. and Veljović, Đorđe and Miletić, Vesna",
year = "2019",
abstract = "ObjectivesThe study aims to evaluate the effects of non-thermal atmospheric plasma (NTAP) treatments on dentin wetting and surface free energy (SFE) and compare the effects of NTAP treatment, etch-and-rinse, and self-etch protocols for application of universal adhesives.Materials and methodsMid-coronal dentin of intact third molars was used to measure contact angles of distilled water, ethylene-glycol, and diiodomethane and calculate SFE following different NTAP preset treatments (feeding gas consisting of pure He, He + 1% O-2, He + 1.5% O-2), power input (1 or 3W), and tip-to-surface distance (2, 4, or 8mm). Contact angles of reference liquids and SFE of dentin following He + 1.5% O-2 at 3-W and 4-mm treatment was compared to phosphoric acid etching. Contact angles of Single Bond Universal (SBU; 3M ESPE) and Clearfil Universal Bond (CUB; Kuraray Noritake) were measured following NTAP, etch-and-rinse, and self-etch protocols.ResultsNTAP significantly reduced contact angles of reference liquids and increased dentin SFE compared to untreated dentin (p lt 0.05). O-2 intensified the effect of He NTAP (p lt 0.05). NTAP and phosphoric acid increased dentin polarity and Lewis base surface characteristics. Phosphoric acid increased contact angles of adhesives compared to the self-etch protocol (p lt 0.05). NTAP resulted in lower adhesive contact angles than phosphoric acid, the difference being statistically significant for CUB (p lt 0.05). Compared to the self-etch protocol, NTAP slightly reduced CUB contact angle but not that of SBU (p>0.05).ConclusionsHe NTAP with and without O-2 increased dentin wetting and SFE, surpassing the effect of phosphoric acid and lowering adhesive contact angles. NTAP produced no apparent micro-morphological changes on dentin surface comparable to acid etching.Clinical significanceNTAP treatment of dentin prior to adhesive application increases dentin wetting and surface free energy facilitating better adhesive distribution on dentin surface compared to phosphoric acid etching and similar to the self-etch application protocol.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives",
volume = "23",
number = "3",
pages = "1383-1396",
doi = "10.1007/s00784-018-2563-2"
}

Stasić, J., Selaković, N., Puač, N., Miletić, M., Malović, G., Petrović, Z. Lj., Veljović, Đ.,& Miletić, V.. (2019). Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 23(3), 1383-1396.
https://doi.org/10.1007/s00784-018-2563-2

Stasić J, Selaković N, Puač N, Miletić M, Malović G, Petrović ZL, Veljović Đ, Miletić V. Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives. in Clinical Oral Investigations. 2019;23(3):1383-1396.
doi:10.1007/s00784-018-2563-2 .

Stasić, Jovana, Selaković, Nenad, Puač, Nevena, Miletić, Maja, Malović, Gordana, Petrović, Zoran Lj., Veljović, Đorđe, Miletić, Vesna, "Effects of non-thermal atmospheric plasma treatment on dentin wetting and surface free energy for application of universal adhesives" in Clinical Oral Investigations, 23, no. 3 (2019):1383-1396,
https://doi.org/10.1007/s00784-018-2563-2 . .

TY  - JOUR
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Mojović, Miloš
AU  - Popović-Bijelić, Ana
AU  - Vuković, Dragana
AU  - Miličić, Biljana
AU  - Maletić, Dejan
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1966
AB  - In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Open Chemistry
T1  - Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment
VL  - 13
IS  - 1
SP  - 332
EP  - 338
DO  - 10.1515/chem-2015-0041
ER  - 

@article{
author = "Puač, Nevena and Miletić, Maja and Mojović, Miloš and Popović-Bijelić, Ana and Vuković, Dragana and Miličić, Biljana and Maletić, Dejan and Lazović, Saša and Malović, Gordana and Petrović, Zoran Lj.",
year = "2015",
abstract = "In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Open Chemistry",
title = "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment",
volume = "13",
number = "1",
pages = "332-338",
doi = "10.1515/chem-2015-0041"
}

Puač, N., Miletić, M., Mojović, M., Popović-Bijelić, A., Vuković, D., Miličić, B., Maletić, D., Lazović, S., Malović, G.,& Petrović, Z. Lj.. (2015). Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry
De Gruyter Open Ltd, Warsaw., 13(1), 332-338.
https://doi.org/10.1515/chem-2015-0041

Puač N, Miletić M, Mojović M, Popović-Bijelić A, Vuković D, Miličić B, Maletić D, Lazović S, Malović G, Petrović ZL. Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment. in Open Chemistry. 2015;13(1):332-338.
doi:10.1515/chem-2015-0041 .

Puač, Nevena, Miletić, Maja, Mojović, Miloš, Popović-Bijelić, Ana, Vuković, Dragana, Miličić, Biljana, Maletić, Dejan, Lazović, Saša, Malović, Gordana, Petrović, Zoran Lj., "Sterilization of bacteria suspensions and identification of radicals deposited during plasma treatment" in Open Chemistry, 13, no. 1 (2015):332-338,
https://doi.org/10.1515/chem-2015-0041 . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Vuković, Dragana
AU  - Zivanović, Irena
AU  - Dakić, Ivana
AU  - Soldatović, Ivan
AU  - Maletić, Dejan
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Petrović, Zoran Lj.
AU  - Puač, Nevena
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1947
AB  - In numerous recent papers plasma chemistry of non equilibrium plasma sources operating at atmospheric pressure has been linked to plasma medical effects including sterilization. In this paper we present a study of the effectiveness of an atmospheric pressure plasma source, known as plasma needle, in inhibition of the growth of biofilm produced by methicillin resistant Staphylococcus aureus (MRSA). Even at the lowest powers the biofilms formed by inoculi of MRSA of 10(4) and 10(5) CFU have been strongly affected by plasma and growth in biofilms was inhibited. The eradication of the already formed biofilm was not achieved and it is required to go to more effective sources.
PB  - De Gruyter Open Ltd, Warsaw
T2  - Central European Journal of Physics
T1  - Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle
VL  - 12
IS  - 3
SP  - 160
EP  - 167
DO  - 10.2478/s11534-014-0437-z
ER  - 

@article{
author = "Miletić, Maja and Vuković, Dragana and Zivanović, Irena and Dakić, Ivana and Soldatović, Ivan and Maletić, Dejan and Lazović, Saša and Malović, Gordana and Petrović, Zoran Lj. and Puač, Nevena",
year = "2014",
abstract = "In numerous recent papers plasma chemistry of non equilibrium plasma sources operating at atmospheric pressure has been linked to plasma medical effects including sterilization. In this paper we present a study of the effectiveness of an atmospheric pressure plasma source, known as plasma needle, in inhibition of the growth of biofilm produced by methicillin resistant Staphylococcus aureus (MRSA). Even at the lowest powers the biofilms formed by inoculi of MRSA of 10(4) and 10(5) CFU have been strongly affected by plasma and growth in biofilms was inhibited. The eradication of the already formed biofilm was not achieved and it is required to go to more effective sources.",
publisher = "De Gruyter Open Ltd, Warsaw",
journal = "Central European Journal of Physics",
title = "Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle",
volume = "12",
number = "3",
pages = "160-167",
doi = "10.2478/s11534-014-0437-z"
}

Miletić, M., Vuković, D., Zivanović, I., Dakić, I., Soldatović, I., Maletić, D., Lazović, S., Malović, G., Petrović, Z. Lj.,& Puač, N.. (2014). Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle. in Central European Journal of Physics
De Gruyter Open Ltd, Warsaw., 12(3), 160-167.
https://doi.org/10.2478/s11534-014-0437-z

Miletić M, Vuković D, Zivanović I, Dakić I, Soldatović I, Maletić D, Lazović S, Malović G, Petrović ZL, Puač N. Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle. in Central European Journal of Physics. 2014;12(3):160-167.
doi:10.2478/s11534-014-0437-z .

Miletić, Maja, Vuković, Dragana, Zivanović, Irena, Dakić, Ivana, Soldatović, Ivan, Maletić, Dejan, Lazović, Saša, Malović, Gordana, Petrović, Zoran Lj., Puač, Nevena, "Inhibition of methicillin resistant Staphylococcus aureus by a plasma needle" in Central European Journal of Physics, 12, no. 3 (2014):160-167,
https://doi.org/10.2478/s11534-014-0437-z . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okic-Đorđević, Ivana
AU  - Maletić, D.
AU  - Puač, Nevena
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Milenković, P.
AU  - Petrović, Zoran Lj.
AU  - Bugarski, Diana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1833
AB  - Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.
PB  - Iop Publishing Ltd, Bristol
T2  - Journal of Physics D - Applied Physics
T1  - Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells
VL  - 46
IS  - 34
DO  - 10.1088/0022-3727/46/34/345401
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okic-Đorđević, Ivana and Maletić, D. and Puač, Nevena and Lazović, Saša and Malović, Gordana and Milenković, P. and Petrović, Zoran Lj. and Bugarski, Diana",
year = "2013",
abstract = "Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Journal of Physics D - Applied Physics",
title = "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells",
volume = "46",
number = "34",
doi = "10.1088/0022-3727/46/34/345401"
}

Miletić, M., Mojsilović, S., Okic-Đorđević, I., Maletić, D., Puač, N., Lazović, S., Malović, G., Milenković, P., Petrović, Z. Lj.,& Bugarski, D.. (2013). Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D - Applied Physics
Iop Publishing Ltd, Bristol., 46(34).
https://doi.org/10.1088/0022-3727/46/34/345401

Miletić M, Mojsilović S, Okic-Đorđević I, Maletić D, Puač N, Lazović S, Malović G, Milenković P, Petrović ZL, Bugarski D. Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D - Applied Physics. 2013;46(34).
doi:10.1088/0022-3727/46/34/345401 .

Miletić, Maja, Mojsilović, S., Okic-Đorđević, Ivana, Maletić, D., Puač, Nevena, Lazović, Saša, Malović, Gordana, Milenković, P., Petrović, Zoran Lj., Bugarski, Diana, "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells" in Journal of Physics D - Applied Physics, 46, no. 34 (2013),
https://doi.org/10.1088/0022-3727/46/34/345401 . .

TY  - JOUR
AU  - Petrović, Zoran Lj.
AU  - Puač, Nevena
AU  - Malović, Gordana
AU  - Lazović, Saša
AU  - Maletić, Dejan
AU  - Miletić, Maja
AU  - Mojsilović, Slavko
AU  - Milenković, Pavle
AU  - Bugarski, Diana
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1724
AB  - The potential of plasma applications medicine, the connections to nanotechnologies and the results obtained by our group are reviewed. A special issue in plasma medicine is the development of the plasma sources that would achieve non-equilibrium at atmospheric pressure in an atmospheric gas mixture with no or only marginal heating of the gas, and with desired properties and mechanisms that may be controlled. Our studies have shown that control of radicals or chemically active products of the discharge, such as ROS (reactive oxygen species) and/or NO, may be used to control the growth of the seeds. Simultaneously, a specially designed plasma needle and other sources were shown to be efficient to sterilize not only colonies of bacteria but also plank- tonic samples (microorganisms protected by water) or bio films. Finally, it was shown that a plasma might induce differentiation of stem cells. Non-equilibrium plasmas may be used in detection of different specific markers in medicine. For example proton transfer mass spectroscopy may be employed in the detection of volatile organic compounds without their dissociation and thus as a technique for instantaneous measurement of the presence of markers for numerous diseases.
AB  - U ovom radu dat je pregled primene plazme u medicini, povezanost sa nanotehnologijama i rezultate na ovom polju koje je postigla naša grupa. Poseban problem u plazma medicini je razvoj izvora plazme koji bi radili u neravnotežnim uslovima na atmosferskom pritisku i u smeši gasova kakva je u atmosferi uz zanemarljivo grejanje gasa i sa željenim karakteristikama koje se mogu podešavati po želji. Naša istraživanja su pokazala da se kontrola prisustva radikala i drugih hemijski aktivnih čestica kao što su reaktivne kiseonične čestice (ROS) i/ili NO, može koristiti za kontrolu klijanja semenki. U isto vreme je dokazano za posebno konstruisanu plazma iglu da može efikasno da steriliše ne samo kolonije bakterija već i planktonske uzorke (mikroorganizme zaštićene vodom) pa i biofilmove. Na kraju, mi smo pokazali da plazma može da indukuje diferencijaciju matičnih ćelija. Neravnotežna plazma se može koristiti za detekciju raznih specifičnih markera u medicini. Na primer masena spektroskopija na bazi izmene protona može da se koristi za detekciju isparivih organskih jedinjenja bez njihove disocijacije i na taj način se može ostvariti trenutna detekcija markera za brojne bolesti iz daha.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Application of non-equilibrium plasmas in medicine
T1  - Primena neravnotežne plazme u medicini
VL  - 77
IS  - 12
SP  - 1689
EP  - 1699
DO  - 10.2298/JSC121020142P
ER  - 

@article{
author = "Petrović, Zoran Lj. and Puač, Nevena and Malović, Gordana and Lazović, Saša and Maletić, Dejan and Miletić, Maja and Mojsilović, Slavko and Milenković, Pavle and Bugarski, Diana",
year = "2012",
abstract = "The potential of plasma applications medicine, the connections to nanotechnologies and the results obtained by our group are reviewed. A special issue in plasma medicine is the development of the plasma sources that would achieve non-equilibrium at atmospheric pressure in an atmospheric gas mixture with no or only marginal heating of the gas, and with desired properties and mechanisms that may be controlled. Our studies have shown that control of radicals or chemically active products of the discharge, such as ROS (reactive oxygen species) and/or NO, may be used to control the growth of the seeds. Simultaneously, a specially designed plasma needle and other sources were shown to be efficient to sterilize not only colonies of bacteria but also plank- tonic samples (microorganisms protected by water) or bio films. Finally, it was shown that a plasma might induce differentiation of stem cells. Non-equilibrium plasmas may be used in detection of different specific markers in medicine. For example proton transfer mass spectroscopy may be employed in the detection of volatile organic compounds without their dissociation and thus as a technique for instantaneous measurement of the presence of markers for numerous diseases., U ovom radu dat je pregled primene plazme u medicini, povezanost sa nanotehnologijama i rezultate na ovom polju koje je postigla naša grupa. Poseban problem u plazma medicini je razvoj izvora plazme koji bi radili u neravnotežnim uslovima na atmosferskom pritisku i u smeši gasova kakva je u atmosferi uz zanemarljivo grejanje gasa i sa željenim karakteristikama koje se mogu podešavati po želji. Naša istraživanja su pokazala da se kontrola prisustva radikala i drugih hemijski aktivnih čestica kao što su reaktivne kiseonične čestice (ROS) i/ili NO, može koristiti za kontrolu klijanja semenki. U isto vreme je dokazano za posebno konstruisanu plazma iglu da može efikasno da steriliše ne samo kolonije bakterija već i planktonske uzorke (mikroorganizme zaštićene vodom) pa i biofilmove. Na kraju, mi smo pokazali da plazma može da indukuje diferencijaciju matičnih ćelija. Neravnotežna plazma se može koristiti za detekciju raznih specifičnih markera u medicini. Na primer masena spektroskopija na bazi izmene protona može da se koristi za detekciju isparivih organskih jedinjenja bez njihove disocijacije i na taj način se može ostvariti trenutna detekcija markera za brojne bolesti iz daha.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Application of non-equilibrium plasmas in medicine, Primena neravnotežne plazme u medicini",
volume = "77",
number = "12",
pages = "1689-1699",
doi = "10.2298/JSC121020142P"
}

Petrović, Z. Lj., Puač, N., Malović, G., Lazović, S., Maletić, D., Miletić, M., Mojsilović, S., Milenković, P.,& Bugarski, D.. (2012). Application of non-equilibrium plasmas in medicine. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(12), 1689-1699.
https://doi.org/10.2298/JSC121020142P

Petrović ZL, Puač N, Malović G, Lazović S, Maletić D, Miletić M, Mojsilović S, Milenković P, Bugarski D. Application of non-equilibrium plasmas in medicine. in Journal of the Serbian Chemical Society. 2012;77(12):1689-1699.
doi:10.2298/JSC121020142P .

Petrović, Zoran Lj., Puač, Nevena, Malović, Gordana, Lazović, Saša, Maletić, Dejan, Miletić, Maja, Mojsilović, Slavko, Milenković, Pavle, Bugarski, Diana, "Application of non-equilibrium plasmas in medicine" in Journal of the Serbian Chemical Society, 77, no. 12 (2012):1689-1699,
https://doi.org/10.2298/JSC121020142P . .

TY  - JOUR
AU  - Lazović, Saša
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Pavlica, Dušan
AU  - Jovanović, Milena
AU  - Bugarski, Diana
AU  - Mojsilović, Slavko
AU  - Maletić, Dejan
AU  - Malović, Gordana
AU  - Milenković, Pavle
AU  - Petrović, Zoran Lj.
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1559
AB  - In this paper, we study the application of a plasma needle to induce necrosis in planktonic samples containing a single breed of bacteria. Two different types of bacteria, Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), were covered in this study. In all experiments with bacteria, the samples were liquid suspensions of several different concentrations of bacteria prepared according to the McFarland standard. The second system studied in this paper was human peripheral blood mesenchymal stem cells (hPB-MSC). In the case of hPB-MSC, two sets of experiments were performed: when cells were covered with a certain amount of liquid (indirect) and when the cell sample was in direct contact with the plasma. Most importantly, the study is made with the aim to see the effects when the living cells are in a liquid medium, which normally acts as protection against the many agents that may be released by plasmas. It was found that a good effect may be expected for a wide range of initial cell densities and operating conditions causing destruction of several orders of magnitude even under the protection of a liquid. It was established independently that a temperature increase could not affect the cells under the conditions of our experiment, so the effect could those hPB-MSC that were not protected by a liquid, gas flow proved to produce a considerable effect, presumably due to poor adhesion of the cells, but in a liquid the effect was only due to the plasma. Further optimization of the operation may be attempted, opening up the possibility of localized in vivo sterilization.
PB  - Iop Publishing Ltd, Bristol
T2  - New Journal of Physics
T1  - The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells
VL  - 12
DO  - 10.1088/1367-2630/12/8/083037
ER  - 

@article{
author = "Lazović, Saša and Puač, Nevena and Miletić, Maja and Pavlica, Dušan and Jovanović, Milena and Bugarski, Diana and Mojsilović, Slavko and Maletić, Dejan and Malović, Gordana and Milenković, Pavle and Petrović, Zoran Lj.",
year = "2010",
abstract = "In this paper, we study the application of a plasma needle to induce necrosis in planktonic samples containing a single breed of bacteria. Two different types of bacteria, Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), were covered in this study. In all experiments with bacteria, the samples were liquid suspensions of several different concentrations of bacteria prepared according to the McFarland standard. The second system studied in this paper was human peripheral blood mesenchymal stem cells (hPB-MSC). In the case of hPB-MSC, two sets of experiments were performed: when cells were covered with a certain amount of liquid (indirect) and when the cell sample was in direct contact with the plasma. Most importantly, the study is made with the aim to see the effects when the living cells are in a liquid medium, which normally acts as protection against the many agents that may be released by plasmas. It was found that a good effect may be expected for a wide range of initial cell densities and operating conditions causing destruction of several orders of magnitude even under the protection of a liquid. It was established independently that a temperature increase could not affect the cells under the conditions of our experiment, so the effect could those hPB-MSC that were not protected by a liquid, gas flow proved to produce a considerable effect, presumably due to poor adhesion of the cells, but in a liquid the effect was only due to the plasma. Further optimization of the operation may be attempted, opening up the possibility of localized in vivo sterilization.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "New Journal of Physics",
title = "The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells",
volume = "12",
doi = "10.1088/1367-2630/12/8/083037"
}

Lazović, S., Puač, N., Miletić, M., Pavlica, D., Jovanović, M., Bugarski, D., Mojsilović, S., Maletić, D., Malović, G., Milenković, P.,& Petrović, Z. Lj.. (2010). The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells. in New Journal of Physics
Iop Publishing Ltd, Bristol., 12.
https://doi.org/10.1088/1367-2630/12/8/083037

Lazović S, Puač N, Miletić M, Pavlica D, Jovanović M, Bugarski D, Mojsilović S, Maletić D, Malović G, Milenković P, Petrović ZL. The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells. in New Journal of Physics. 2010;12.
doi:10.1088/1367-2630/12/8/083037 .

Lazović, Saša, Puač, Nevena, Miletić, Maja, Pavlica, Dušan, Jovanović, Milena, Bugarski, Diana, Mojsilović, Slavko, Maletić, Dejan, Malović, Gordana, Milenković, Pavle, Petrović, Zoran Lj., "The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells" in New Journal of Physics, 12 (2010),
https://doi.org/10.1088/1367-2630/12/8/083037 . .