TY  - JOUR
AU  - Kannosh, Ibrahim
AU  - Staletović, Danijela
AU  - Toljić, Boško
AU  - Radunović, Milena
AU  - Pucar, Ana
AU  - Matić-Petrović, Sanja
AU  - Grubiša, Ivana
AU  - Lazarević, Miloš
AU  - Brkić, Zlata
AU  - Knežević-Vukcević, Jelena
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2276
AB  - Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.
PB  - J Infection Developing Countries, Tramaniglio
T2  - Journal of Infection in Developing Countries
T1  - The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis
VL  - 12
IS  - 12
SP  - 1088
EP  - 1095
DO  - 10.3855/jidc.10980
ER  - 

@article{
author = "Kannosh, Ibrahim and Staletović, Danijela and Toljić, Boško and Radunović, Milena and Pucar, Ana and Matić-Petrović, Sanja and Grubiša, Ivana and Lazarević, Miloš and Brkić, Zlata and Knežević-Vukcević, Jelena and Milašin, Jelena",
year = "2018",
abstract = "Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis. Methodology: The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td). Results: Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%: 53%), Pi (71%: 31%), Pg (60%: 38%), Aa (39%: 14%) and Td (21%: 6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis. Conclusions: Patient's age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.",
publisher = "J Infection Developing Countries, Tramaniglio",
journal = "Journal of Infection in Developing Countries",
title = "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis",
volume = "12",
number = "12",
pages = "1088-1095",
doi = "10.3855/jidc.10980"
}

Kannosh, I., Staletović, D., Toljić, B., Radunović, M., Pucar, A., Matić-Petrović, S., Grubiša, I., Lazarević, M., Brkić, Z., Knežević-Vukcević, J.,& Milašin, J.. (2018). The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries
J Infection Developing Countries, Tramaniglio., 12(12), 1088-1095.
https://doi.org/10.3855/jidc.10980

Kannosh I, Staletović D, Toljić B, Radunović M, Pucar A, Matić-Petrović S, Grubiša I, Lazarević M, Brkić Z, Knežević-Vukcević J, Milašin J. The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis. in Journal of Infection in Developing Countries. 2018;12(12):1088-1095.
doi:10.3855/jidc.10980 .

Kannosh, Ibrahim, Staletović, Danijela, Toljić, Boško, Radunović, Milena, Pucar, Ana, Matić-Petrović, Sanja, Grubiša, Ivana, Lazarević, Miloš, Brkić, Zlata, Knežević-Vukcević, Jelena, Milašin, Jelena, "The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques- An age related comparative analysis" in Journal of Infection in Developing Countries, 12, no. 12 (2018):1088-1095,
https://doi.org/10.3855/jidc.10980 . .

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Petrović, V.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
AU  - Nikolić, B.
AU  - Knežević-Vukcević, Jelena
AU  - Mitić-Ćulafić, Dragana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1838
AB  - Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes
VL  - 46
IS  - 6
SP  - 506
EP  - 516
DO  - 10.1111/iej.12017
ER  - 

@article{
author = "Opačić-Galić, Vanja and Petrović, V. and Živković, Slavoljub and Jokanović, Vukoman and Nikolić, B. and Knežević-Vukcević, Jelena and Mitić-Ćulafić, Dragana",
year = "2013",
abstract = "Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes",
volume = "46",
number = "6",
pages = "506-516",
doi = "10.1111/iej.12017"
}

Opačić-Galić, V., Petrović, V., Živković, S., Jokanović, V., Nikolić, B., Knežević-Vukcević, J.,& Mitić-Ćulafić, D.. (2013). New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal
Wiley, Hoboken., 46(6), 506-516.
https://doi.org/10.1111/iej.12017

Opačić-Galić V, Petrović V, Živković S, Jokanović V, Nikolić B, Knežević-Vukcević J, Mitić-Ćulafić D. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal. 2013;46(6):506-516.
doi:10.1111/iej.12017 .

Opačić-Galić, Vanja, Petrović, V., Živković, Slavoljub, Jokanović, Vukoman, Nikolić, B., Knežević-Vukcević, Jelena, Mitić-Ćulafić, Dragana, "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes" in International Endodontic Journal, 46, no. 6 (2013):506-516,
https://doi.org/10.1111/iej.12017 . .