TY  - JOUR
AU  - Radunović, Milena
AU  - Petrini, M.
AU  - Vlajić, T.
AU  - Iezzi, G.
AU  - Di Lodovico, S.
AU  - Piattelli, Adriano
AU  - D'Ercole, S.
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2509
AB  - Antibiotic resistance is a major public health problem worldwide and the finding of alternative methods for eliminating bacteria is one of the prerogatives of medical research. The indiscriminate use of antibiotics in dentistry, especially for the treatment of peri-implantitis, could lead to superinfections. Alternative methods, like photodynamic therapy mediated by the use of aminolevulinic acid and a red light has been largely described, especially in dentistry, but results were encouraging against Gram-positive bacteria, but limited against Gram-negative. The effectiveness of photodynamic therapy mediated by a novel product containing aminolevulinic acid, Aladent (ALAD) has been tested in this in vitro study, against different types of bacteria particularly involved in the infections of the oral cavity and peri-implantitis. The novelty of ALAD is the marked hydrophilicity that should increase the passage of the molecule through the membrane pores of Gram-negative bacteria. Considering the novelty of the product a preliminary experiment permitted to test the effectiveness against Enterococcus faecalis after 1 h of ALAD incubation at different concentrations, with or without different timings of LED irradiation. The count of CFUs and the live/dead observation with fluorescent microscopy showed a significant reduction and killing of bacterium. Then, in the second stage, that could meet the necessity of effectiveness and the clinician's requests to reduce the timing of treatment, ALAD, with and without irradiation, was tested on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Veillonella parvula and Porphyromonas gingivalis. In particular, the efficiency of different concentrations of the product after a 25 min incubation was tested with and without the adjunctive LED irradiation for 5 min. A slight ALAD bactericidal effect was reported for all bacteria, also without LED irradiation, however, the most effective treatment was 25 min of 50% ALAD incubation followed by 5 min of a red LED. The in vitro tests demonstrated that ALAD gel with LED irradiation exerts a potent antibacterial activity on different bacteria, both Gram-positive and Gram-negative.
PB  - Elsevier Science Sa, Lausanne
T2  - Journal of Photochemistry & Photobiology B-Biology
T1  - Effects of a novel gel containing 5-aminolevulinic acid and red LED against bacteria involved in peri-implantitis and other oral infections
VL  - 205
DO  - 10.1016/j.jphotobiol.2020.111826
ER  - 

@article{
author = "Radunović, Milena and Petrini, M. and Vlajić, T. and Iezzi, G. and Di Lodovico, S. and Piattelli, Adriano and D'Ercole, S.",
year = "2020",
abstract = "Antibiotic resistance is a major public health problem worldwide and the finding of alternative methods for eliminating bacteria is one of the prerogatives of medical research. The indiscriminate use of antibiotics in dentistry, especially for the treatment of peri-implantitis, could lead to superinfections. Alternative methods, like photodynamic therapy mediated by the use of aminolevulinic acid and a red light has been largely described, especially in dentistry, but results were encouraging against Gram-positive bacteria, but limited against Gram-negative. The effectiveness of photodynamic therapy mediated by a novel product containing aminolevulinic acid, Aladent (ALAD) has been tested in this in vitro study, against different types of bacteria particularly involved in the infections of the oral cavity and peri-implantitis. The novelty of ALAD is the marked hydrophilicity that should increase the passage of the molecule through the membrane pores of Gram-negative bacteria. Considering the novelty of the product a preliminary experiment permitted to test the effectiveness against Enterococcus faecalis after 1 h of ALAD incubation at different concentrations, with or without different timings of LED irradiation. The count of CFUs and the live/dead observation with fluorescent microscopy showed a significant reduction and killing of bacterium. Then, in the second stage, that could meet the necessity of effectiveness and the clinician's requests to reduce the timing of treatment, ALAD, with and without irradiation, was tested on Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Veillonella parvula and Porphyromonas gingivalis. In particular, the efficiency of different concentrations of the product after a 25 min incubation was tested with and without the adjunctive LED irradiation for 5 min. A slight ALAD bactericidal effect was reported for all bacteria, also without LED irradiation, however, the most effective treatment was 25 min of 50% ALAD incubation followed by 5 min of a red LED. The in vitro tests demonstrated that ALAD gel with LED irradiation exerts a potent antibacterial activity on different bacteria, both Gram-positive and Gram-negative.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Journal of Photochemistry & Photobiology B-Biology",
title = "Effects of a novel gel containing 5-aminolevulinic acid and red LED against bacteria involved in peri-implantitis and other oral infections",
volume = "205",
doi = "10.1016/j.jphotobiol.2020.111826"
}

Radunović, M., Petrini, M., Vlajić, T., Iezzi, G., Di Lodovico, S., Piattelli, A.,& D'Ercole, S.. (2020). Effects of a novel gel containing 5-aminolevulinic acid and red LED against bacteria involved in peri-implantitis and other oral infections. in Journal of Photochemistry & Photobiology B-Biology
Elsevier Science Sa, Lausanne., 205.
https://doi.org/10.1016/j.jphotobiol.2020.111826

Radunović M, Petrini M, Vlajić T, Iezzi G, Di Lodovico S, Piattelli A, D'Ercole S. Effects of a novel gel containing 5-aminolevulinic acid and red LED against bacteria involved in peri-implantitis and other oral infections. in Journal of Photochemistry & Photobiology B-Biology. 2020;205.
doi:10.1016/j.jphotobiol.2020.111826 .

Radunović, Milena, Petrini, M., Vlajić, T., Iezzi, G., Di Lodovico, S., Piattelli, Adriano, D'Ercole, S., "Effects of a novel gel containing 5-aminolevulinic acid and red LED against bacteria involved in peri-implantitis and other oral infections" in Journal of Photochemistry & Photobiology B-Biology, 205 (2020),
https://doi.org/10.1016/j.jphotobiol.2020.111826 . .

TY  - JOUR
AU  - De Colli, Marianna
AU  - Radunović, Milena
AU  - Zizzari, Vincenzo L.
AU  - Di Giacomo, Viviana
AU  - Di Nisio, Chiara
AU  - Piattelli, Adriano
AU  - Calvo Guirado, Jose Luis
AU  - Zavan, Barbara
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2304
AB  - Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.
PB  - Japanese Soc Dental Materials Devices, Tokyo
T2  - Dental Materials Journal
T1  - Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface
VL  - 37
IS  - 2
SP  - 197
EP  - 205
DO  - 10.4012/dmj.2016-418
ER  - 

@article{
author = "De Colli, Marianna and Radunović, Milena and Zizzari, Vincenzo L. and Di Giacomo, Viviana and Di Nisio, Chiara and Piattelli, Adriano and Calvo Guirado, Jose Luis and Zavan, Barbara and Cataldi, Amelia and Zara, Susi",
year = "2018",
abstract = "Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.",
publisher = "Japanese Soc Dental Materials Devices, Tokyo",
journal = "Dental Materials Journal",
title = "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface",
volume = "37",
number = "2",
pages = "197-205",
doi = "10.4012/dmj.2016-418"
}

De Colli, M., Radunović, M., Zizzari, V. L., Di Giacomo, V., Di Nisio, C., Piattelli, A., Calvo Guirado, J. L., Zavan, B., Cataldi, A.,& Zara, S.. (2018). Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal
Japanese Soc Dental Materials Devices, Tokyo., 37(2), 197-205.
https://doi.org/10.4012/dmj.2016-418

De Colli M, Radunović M, Zizzari VL, Di Giacomo V, Di Nisio C, Piattelli A, Calvo Guirado JL, Zavan B, Cataldi A, Zara S. Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal. 2018;37(2):197-205.
doi:10.4012/dmj.2016-418 .

De Colli, Marianna, Radunović, Milena, Zizzari, Vincenzo L., Di Giacomo, Viviana, Di Nisio, Chiara, Piattelli, Adriano, Calvo Guirado, Jose Luis, Zavan, Barbara, Cataldi, Amelia, Zara, Susi, "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface" in Dental Materials Journal, 37, no. 2 (2018):197-205,
https://doi.org/10.4012/dmj.2016-418 . .

TY  - JOUR
AU  - De Marco, Patrizia
AU  - Zara, Susi
AU  - De Colli, Marianna
AU  - Radunović, Milena
AU  - Lazović, Vladimir
AU  - Ettorre, Valeria
AU  - Di Crescenzo, Antonello
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Fontana, Antonella
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2218
AB  - Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.
PB  - Iop Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts
VL  - 12
IS  - 5
DO  - 10.1088/1748-605X/aa7907
ER  - 

@article{
author = "De Marco, Patrizia and Zara, Susi and De Colli, Marianna and Radunović, Milena and Lazović, Vladimir and Ettorre, Valeria and Di Crescenzo, Antonello and Piattelli, Adriano and Cataldi, Amelia and Fontana, Antonella",
year = "2017",
abstract = "Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts",
volume = "12",
number = "5",
doi = "10.1088/1748-605X/aa7907"
}

De Marco, P., Zara, S., De Colli, M., Radunović, M., Lazović, V., Ettorre, V., Di Crescenzo, A., Piattelli, A., Cataldi, A.,& Fontana, A.. (2017). Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials
Iop Publishing Ltd, Bristol., 12(5).
https://doi.org/10.1088/1748-605X/aa7907

De Marco P, Zara S, De Colli M, Radunović M, Lazović V, Ettorre V, Di Crescenzo A, Piattelli A, Cataldi A, Fontana A. Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials. 2017;12(5).
doi:10.1088/1748-605X/aa7907 .

De Marco, Patrizia, Zara, Susi, De Colli, Marianna, Radunović, Milena, Lazović, Vladimir, Ettorre, Valeria, Di Crescenzo, Antonello, Piattelli, Adriano, Cataldi, Amelia, Fontana, Antonella, "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts" in Biomedical Materials, 12, no. 5 (2017),
https://doi.org/10.1088/1748-605X/aa7907 . .

TY  - JOUR
AU  - Gehrke, Sergio A.
AU  - Perez-Albacete, Carlos
AU  - Martinez
AU  - Piattelli, Adriano
AU  - Shibli, Jamil A.
AU  - Marković, Aleksa
AU  - Calvo Guirado, Jose Luis
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2193
AB  - Objectives: The aim of this study was to perform a histomorphometric and biomechanical comparison of three implants with different designs of the apical area to promote a better bone initial stability and its correlation with the osseointegration. Material and methods: Fifty-four tapered implants with same length, diameter and surface properties but with three different apical configurations (Group I: MK4: Group II: C1 and Group III: MK7) were inserted in the tibia of rabbits. Implant stability and bone formation were evaluated by resonance frequency analysis measured at 0, 6, 8 and 12 weeks and by histomorphometric analysis performed at 6, 8 and 12 weeks. Results: Statistical test to compare the stability through the implant stability quotient in the four times showed few differences between the groups and time periods proposed, with significance set at P  lt  0.05. In the bone-implant contact, by comparing the groups in the three times proposed, it was possible concluded that there is a similar behavior among the three implant design (P  lt  0.05). Conclusion: With the limitations of this animal study, it can be concluded that the design of the apical area influences the implant stability and the bone-to-implant contact.
PB  - Wiley, Hoboken
T2  - Clinical Oral Implants Research
T1  - The influence of three different apical implant designs at stability and osseointegration process: experimental study in rabbits
VL  - 28
IS  - 3
SP  - 355
EP  - 361
DO  - 10.1111/clr.12807
ER  - 

@article{
author = "Gehrke, Sergio A. and Perez-Albacete, Carlos and Martinez and Piattelli, Adriano and Shibli, Jamil A. and Marković, Aleksa and Calvo Guirado, Jose Luis",
year = "2017",
abstract = "Objectives: The aim of this study was to perform a histomorphometric and biomechanical comparison of three implants with different designs of the apical area to promote a better bone initial stability and its correlation with the osseointegration. Material and methods: Fifty-four tapered implants with same length, diameter and surface properties but with three different apical configurations (Group I: MK4: Group II: C1 and Group III: MK7) were inserted in the tibia of rabbits. Implant stability and bone formation were evaluated by resonance frequency analysis measured at 0, 6, 8 and 12 weeks and by histomorphometric analysis performed at 6, 8 and 12 weeks. Results: Statistical test to compare the stability through the implant stability quotient in the four times showed few differences between the groups and time periods proposed, with significance set at P  lt  0.05. In the bone-implant contact, by comparing the groups in the three times proposed, it was possible concluded that there is a similar behavior among the three implant design (P  lt  0.05). Conclusion: With the limitations of this animal study, it can be concluded that the design of the apical area influences the implant stability and the bone-to-implant contact.",
publisher = "Wiley, Hoboken",
journal = "Clinical Oral Implants Research",
title = "The influence of three different apical implant designs at stability and osseointegration process: experimental study in rabbits",
volume = "28",
number = "3",
pages = "355-361",
doi = "10.1111/clr.12807"
}

Gehrke, S. A., Perez-Albacete, C., Martinez, Piattelli, A., Shibli, J. A., Marković, A.,& Calvo Guirado, J. L.. (2017). The influence of three different apical implant designs at stability and osseointegration process: experimental study in rabbits. in Clinical Oral Implants Research
Wiley, Hoboken., 28(3), 355-361.
https://doi.org/10.1111/clr.12807

Gehrke SA, Perez-Albacete C, Martinez, Piattelli A, Shibli JA, Marković A, Calvo Guirado JL. The influence of three different apical implant designs at stability and osseointegration process: experimental study in rabbits. in Clinical Oral Implants Research. 2017;28(3):355-361.
doi:10.1111/clr.12807 .

Gehrke, Sergio A., Perez-Albacete, Carlos, Martinez, Piattelli, Adriano, Shibli, Jamil A., Marković, Aleksa, Calvo Guirado, Jose Luis, "The influence of three different apical implant designs at stability and osseointegration process: experimental study in rabbits" in Clinical Oral Implants Research, 28, no. 3 (2017):355-361,
https://doi.org/10.1111/clr.12807 . .

TY  - JOUR
AU  - Radunović, Milena
AU  - De Colli, Marianna
AU  - De Marco, Patrizia
AU  - Di Nisio, Chiara
AU  - Fontana, Antonella
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2186
AB  - Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence
VL  - 105
IS  - 8
SP  - 2312
EP  - 2320
DO  - 10.1002/jbm.a.36085
ER  - 

@article{
author = "Radunović, Milena and De Colli, Marianna and De Marco, Patrizia and Di Nisio, Chiara and Fontana, Antonella and Piattelli, Adriano and Cataldi, Amelia and Zara, Susi",
year = "2017",
abstract = "Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence",
volume = "105",
number = "8",
pages = "2312-2320",
doi = "10.1002/jbm.a.36085"
}

Radunović, M., De Colli, M., De Marco, P., Di Nisio, C., Fontana, A., Piattelli, A., Cataldi, A.,& Zara, S.. (2017). Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 105(8), 2312-2320.
https://doi.org/10.1002/jbm.a.36085

Radunović M, De Colli M, De Marco P, Di Nisio C, Fontana A, Piattelli A, Cataldi A, Zara S. Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A. 2017;105(8):2312-2320.
doi:10.1002/jbm.a.36085 .

Radunović, Milena, De Colli, Marianna, De Marco, Patrizia, Di Nisio, Chiara, Fontana, Antonella, Piattelli, Adriano, Cataldi, Amelia, Zara, Susi, "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence" in Journal of Biomedical Materials Research Part A, 105, no. 8 (2017):2312-2320,
https://doi.org/10.1002/jbm.a.36085 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Marković, Aleksa
AU  - Calvo Guirado, Jose Luis
AU  - Lazić, Zoran
AU  - Piattelli, Adriano
AU  - Boticelli, Daniele
AU  - Maté-Sánchez, José Eduardo
AU  - Negri, Bruno
AU  - Ramírez-Fernández, María Piedad
AU  - Mišić, Tijana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1900
AB  - Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels.
AB  - Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs
T1  - Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima
VL  - 71
IS  - 5
SP  - 451
EP  - 461
DO  - 10.2298/VSP121003034D
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Marković, Aleksa and Calvo Guirado, Jose Luis and Lazić, Zoran and Piattelli, Adriano and Boticelli, Daniele and Maté-Sánchez, José Eduardo and Negri, Bruno and Ramírez-Fernández, María Piedad and Mišić, Tijana",
year = "2014",
abstract = "Background/Aim. The modification of implant surfaces could affect mechanical implant stability as well as dynamics and quality of peri-implant bone healing. The aim of this 3-month experimental study in dogs was to investigate implant stability, marginal bone levels and bone tissue response to zirconia dental implants with two laser-micro-grooved intraosseous surfaces in comparison with nongrooved sandblasted zirconia and sandblasted, high-temperature etched titanium implants. Methods. Implant surface characterization was performed using optical interferometric profilometry and energy dispersive X-ray spectroscopy. A total of 96 implants (4 mm in diameter and 10 mm in length) were inserted randomly in both sides of the lower jaw of 12 Fox Hound dogs divided into groups of 24 each: the control (titanium), the group A (sandblasted zirconia), the group B (sandblasted zirconia plus microgrooved neck) and the group C (sandblasted zirconia plus all microgrooved). All the implants were immediately loaded. Insertion torque, periotest values, radiographic crestal bone level and removal torque were recorded during the 3-month follow-up. Qualitative scanning electon microscope (SEM) analysis of the bone-implant interfaces of each group was performed. Results. Insertion torque values were higher in the group C and control implants (p  lt  0.05). Periotest values increased in all the periods in proportion to the extent of microgrooving as follows: the group C > the control > the group B > the group A (p  lt  0.05). Radiographic measurements showed minimal crestal bone loss at 3 months for microgrooved zirconia implants (groups C and B) and control implants compared with the group A implants (p  lt  0.05). The removal torque values increased with time for all the groups as follows: the group C > the control > the group B > the group A (p  lt  0.05). SEM showed that implant surfaces of the groups B and C had an extra bone growth inside the microgrooves that corresponded to the shape and direction of the microgrooves. Conclusion. The addition of microgrooves to the entire intraosseous surface of zirconia dental implants enhances primary and secondary implant stability, promotes bone tissue in growth and preserves crestal bone levels., Uvod/Cilj. Modifikacija površine implantata može uticati na njegovu mehaničku stabilnost kao i na dinamiku i kvalitet periimplantatnog koštanog zarastanja. Cilj ove tromesečne eksperimentalne studije na psima bio je da se ispita stabilnost implantata, nivo marginalne kosti i odgovor koštanog tkiva na cirkonijum endoosealne implantate sa dve intraosealne površine mikrostrukturirane laserom u poređenju sa peskiranim cirkonijum implantatima čija površina nije mikrostrukturirana kao i sa titanijum implantatima čije su površine peskirane i nagrižene visokom temperaturom. Metode. Karakterizacija površine implantata učinjena je optičkom interferometrijskom profilometrijom i analizom energetskog spektra pri difrakciji X-zračenja. Ukupno 96 implantata (prečnika 4 mm i dužine 10 mm) ugrađeno je nasumično i obostrano u donju vilicu kod 12 pasa (lisičara) i podeljeno u četiri grupe po 24: kontrolna (titanijum implantati); grupa A (peskirani cirkonijum implantati); grupa B (peskirani cirkonijum implantati sa mikrokanalima u koronarnoj trećini); grupa C (peskirani cirkonijum implantati sa mikrokanalima duž cele površine). Svi implantati su odmah opterećeni. Meren je obrtni momenat pri ugradnji implantata, vrednosti periotesta, radiografski nivo marginalne kosti i obrtni moment za uklanjanje implantata tokom tromesečnog perioda praćenja. Međuspoj kosti i implantata iz svake grupe ispitivan je kvalitativnom skenirajućom elektronskom mikroskopijom (SEM). Rezultati. Veći obrtni momenat zabeležen je pri ugradnji implantata kod grupe C i kontrolne grupe (p  lt  0,05). U ispitivanom vremenskom periodu, vrednosti periotesta uvećavale su se srazmerno obimu mikrostrukturiranja površine i to: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Radiografskom analizom utvrđen je minimalni gubitak marginalne kosti u trećem mesecu praćenja oko cirkonijum implantata sa mikrokanalima (grupa B i C) i kontrola u poređenju sa implantatima grupe A (p  lt  0,05). Vrednosti obrtnog momenta za uklanjanje implantata vremenom su se uvećavale u svim grupama na sledeći način: grupa C > kontrolna grupa > grupa B > grupa A (p  lt  0,05). Kod implantatnih površina grupa B i C, SEM je pokazala dodatni rast koštanog tkiva unutar mikrokanala koji odgovara njihovom obliku i pravcu. Zaključak. Formiranje mikrokanala duž cele intraosealne površine cirkonijum endoosealnih implantata povećava primarnu i sekundarnu implantatnu stabilnost, podstiče urastanje koštanog tkiva i održava nivo marginalne kosti.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs, Implantatna stabilnost i nivo marginalne kosti kod cirkonijum endoosealnih implantata sa mikrostrukturiranom površinom - tromesečna eksperimentalna studija na psima",
volume = "71",
number = "5",
pages = "451-461",
doi = "10.2298/VSP121003034D"
}

Delgado-Ruiz, R., Marković, A., Calvo Guirado, J. L., Lazić, Z., Piattelli, A., Boticelli, D., Maté-Sánchez, J. E., Negri, B., Ramírez-Fernández, M. P.,& Mišić, T.. (2014). Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(5), 451-461.
https://doi.org/10.2298/VSP121003034D

Delgado-Ruiz R, Marković A, Calvo Guirado JL, Lazić Z, Piattelli A, Boticelli D, Maté-Sánchez JE, Negri B, Ramírez-Fernández MP, Mišić T. Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs. in Vojnosanitetski pregled. 2014;71(5):451-461.
doi:10.2298/VSP121003034D .

Delgado-Ruiz, Rafael, Marković, Aleksa, Calvo Guirado, Jose Luis, Lazić, Zoran, Piattelli, Adriano, Boticelli, Daniele, Maté-Sánchez, José Eduardo, Negri, Bruno, Ramírez-Fernández, María Piedad, Mišić, Tijana, "Implant stability and marginal bone level of microgrooved zirconia dental implants: A 3-month experimental study on dogs" in Vojnosanitetski pregled, 71, no. 5 (2014):451-461,
https://doi.org/10.2298/VSP121003034D . .