TY  - JOUR
AU  - Trifunović, Jovanka
AU  - Basta-Jovanović, Gordana
AU  - Nikolić, Nadja
AU  - Čarkić, Jelena
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Radojević-Škodrić, Sanja
AU  - Prvanović, Mirjana
AU  - Jovanović, Aleksandar
AU  - Džamić, Zoran
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2272
AB  - Purpose: Renal cell carcinoma (RCC) is the most common renal cancer in adults and includes several subtypes that may be distinguished by their histology, genetic background, clinical course and responses to treatment. Human telomerase reverse transcriptase (hTERT), a crucial enzyme for telomere maintenance, has been linked to RCC development. The purpose of this study was to search for genetic and epigenetic alterations in hTERT (promoter mutations and methylation and gene amplification), and to establish a possible association between molecular and clinico-pathological characteristics of RCC. Methods: DNA was extracted from 31 formalin fixed, para,, n-embedded tumor samples and 23 blood samples from 54 patients with RCC. Polymerase chain reaction (PCR) products were sequenced and analyzed using the Sequencher software. HTERT amplification was determined by quantitative PCR, while the promoter methylation status was assessed by methylation specific PCR. Statistical analysis was performed using SPSS. Results: No mutations could be detected in the hTERT promoter but only a single nucleotide polymorphism (SNP) (-245 T > C). In 54 analyzed RCC cases, the variant allele C was present in homozygous or heterozygous form in 48% of the patients. The C allele was significantly more frequent in low grade tumors (p=0.046). Gene amplification was detected in 19.4% of the 31 RCCs and hTERT methylation in 54.8% of the 31 samples. An association was established between methylation and histological type of RCC (p=0.047). Conclusions: HTERT seems to be implicated in RCC pathogenesis since the promoter polymorphism exerts a modulation effect on tumor behavior. In addition, hTERT promoter methylation status is related to RCC histology.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population
VL  - 23
IS  - 6
SP  - 1887
EP  - 1892
UR  - https://hdl.handle.net/21.15107/rcub_smile_2272
ER  - 

@article{
author = "Trifunović, Jovanka and Basta-Jovanović, Gordana and Nikolić, Nadja and Čarkić, Jelena and Marjanović, Ana and Branković, Marija and Radojević-Škodrić, Sanja and Prvanović, Mirjana and Jovanović, Aleksandar and Džamić, Zoran and Milašin, Jelena",
year = "2018",
abstract = "Purpose: Renal cell carcinoma (RCC) is the most common renal cancer in adults and includes several subtypes that may be distinguished by their histology, genetic background, clinical course and responses to treatment. Human telomerase reverse transcriptase (hTERT), a crucial enzyme for telomere maintenance, has been linked to RCC development. The purpose of this study was to search for genetic and epigenetic alterations in hTERT (promoter mutations and methylation and gene amplification), and to establish a possible association between molecular and clinico-pathological characteristics of RCC. Methods: DNA was extracted from 31 formalin fixed, para,, n-embedded tumor samples and 23 blood samples from 54 patients with RCC. Polymerase chain reaction (PCR) products were sequenced and analyzed using the Sequencher software. HTERT amplification was determined by quantitative PCR, while the promoter methylation status was assessed by methylation specific PCR. Statistical analysis was performed using SPSS. Results: No mutations could be detected in the hTERT promoter but only a single nucleotide polymorphism (SNP) (-245 T > C). In 54 analyzed RCC cases, the variant allele C was present in homozygous or heterozygous form in 48% of the patients. The C allele was significantly more frequent in low grade tumors (p=0.046). Gene amplification was detected in 19.4% of the 31 RCCs and hTERT methylation in 54.8% of the 31 samples. An association was established between methylation and histological type of RCC (p=0.047). Conclusions: HTERT seems to be implicated in RCC pathogenesis since the promoter polymorphism exerts a modulation effect on tumor behavior. In addition, hTERT promoter methylation status is related to RCC histology.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population",
volume = "23",
number = "6",
pages = "1887-1892",
url = "https://hdl.handle.net/21.15107/rcub_smile_2272"
}

Trifunović, J., Basta-Jovanović, G., Nikolić, N., Čarkić, J., Marjanović, A., Branković, M., Radojević-Škodrić, S., Prvanović, M., Jovanović, A., Džamić, Z.,& Milašin, J.. (2018). HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(6), 1887-1892.
https://hdl.handle.net/21.15107/rcub_smile_2272

Trifunović J, Basta-Jovanović G, Nikolić N, Čarkić J, Marjanović A, Branković M, Radojević-Škodrić S, Prvanović M, Jovanović A, Džamić Z, Milašin J. HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population. in Journal of BUON. 2018;23(6):1887-1892.
https://hdl.handle.net/21.15107/rcub_smile_2272 .

Trifunović, Jovanka, Basta-Jovanović, Gordana, Nikolić, Nadja, Čarkić, Jelena, Marjanović, Ana, Branković, Marija, Radojević-Škodrić, Sanja, Prvanović, Mirjana, Jovanović, Aleksandar, Džamić, Zoran, Milašin, Jelena, "HTERT promoter methylation and single nucleotide polymorphism (-245 T > C) affect renal cell carcinoma behavior in Serbian population" in Journal of BUON, 23, no. 6 (2018):1887-1892,
https://hdl.handle.net/21.15107/rcub_smile_2272 .

TY  - JOUR
AU  - Latić, Dragana
AU  - Radojević-Škodrić, Sanja
AU  - Nikolić, Srđan
AU  - Prvanović, Mirjana
AU  - Lazić, Miodrag
AU  - Džamić, Zoran
AU  - Bogdanović, Ljiljana
AU  - Radunović, Milena
AU  - Vuković, Marina
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2206
AB  - Purpose: Renal cell carcinoma (RCC) is the most common malignant kidney tumor in adults. Dysregulation of the cell cycle can lead to cancer development. In this study, the mitosis-associated cyclin A and p16, a negative controller, were investigated as potential key points in the RCC development. Methods: This retrospective study included 74 patients with RCC. The expression of cyclin A and p16 and their correlation to histopathological parameters (TNM stage, histological subtype, nuclear grade, tumor size), gender, age, and clinical outcome were studied and analyzed. Results: The highest median value for cyclin A (40%; range 0-70)) and for p16 (57.5%; range 35-80) were found in the papillary histological subtype. Survival analysis showed that in the group of patients that had died before September 2015, the median value for cyclin A was 20% (range 0-60), which was significantly higher than 5% (range 0-70), found in the group of patients that survived (p=0.019). Conclusions: In relation to the histological subtype, the papillary type of RCC was associated with a significantly higher expression of cyclin A and p16 compared to other subtypes of RCC. High expression of cyclin A indicated worse prognosis, therefore cyclin A could be considered to be a significant prognostic marker.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Immunohistochemical study of cyclin A and p16 expression in patients with renal cell carcinoma
VL  - 22
IS  - 5
SP  - 1322
EP  - 1327
UR  - https://hdl.handle.net/21.15107/rcub_smile_2206
ER  - 

@article{
author = "Latić, Dragana and Radojević-Škodrić, Sanja and Nikolić, Srđan and Prvanović, Mirjana and Lazić, Miodrag and Džamić, Zoran and Bogdanović, Ljiljana and Radunović, Milena and Vuković, Marina",
year = "2017",
abstract = "Purpose: Renal cell carcinoma (RCC) is the most common malignant kidney tumor in adults. Dysregulation of the cell cycle can lead to cancer development. In this study, the mitosis-associated cyclin A and p16, a negative controller, were investigated as potential key points in the RCC development. Methods: This retrospective study included 74 patients with RCC. The expression of cyclin A and p16 and their correlation to histopathological parameters (TNM stage, histological subtype, nuclear grade, tumor size), gender, age, and clinical outcome were studied and analyzed. Results: The highest median value for cyclin A (40%; range 0-70)) and for p16 (57.5%; range 35-80) were found in the papillary histological subtype. Survival analysis showed that in the group of patients that had died before September 2015, the median value for cyclin A was 20% (range 0-60), which was significantly higher than 5% (range 0-70), found in the group of patients that survived (p=0.019). Conclusions: In relation to the histological subtype, the papillary type of RCC was associated with a significantly higher expression of cyclin A and p16 compared to other subtypes of RCC. High expression of cyclin A indicated worse prognosis, therefore cyclin A could be considered to be a significant prognostic marker.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Immunohistochemical study of cyclin A and p16 expression in patients with renal cell carcinoma",
volume = "22",
number = "5",
pages = "1322-1327",
url = "https://hdl.handle.net/21.15107/rcub_smile_2206"
}

Latić, D., Radojević-Škodrić, S., Nikolić, S., Prvanović, M., Lazić, M., Džamić, Z., Bogdanović, L., Radunović, M.,& Vuković, M.. (2017). Immunohistochemical study of cyclin A and p16 expression in patients with renal cell carcinoma. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 22(5), 1322-1327.
https://hdl.handle.net/21.15107/rcub_smile_2206

Latić D, Radojević-Škodrić S, Nikolić S, Prvanović M, Lazić M, Džamić Z, Bogdanović L, Radunović M, Vuković M. Immunohistochemical study of cyclin A and p16 expression in patients with renal cell carcinoma. in Journal of BUON. 2017;22(5):1322-1327.
https://hdl.handle.net/21.15107/rcub_smile_2206 .

Latić, Dragana, Radojević-Škodrić, Sanja, Nikolić, Srđan, Prvanović, Mirjana, Lazić, Miodrag, Džamić, Zoran, Bogdanović, Ljiljana, Radunović, Milena, Vuković, Marina, "Immunohistochemical study of cyclin A and p16 expression in patients with renal cell carcinoma" in Journal of BUON, 22, no. 5 (2017):1322-1327,
https://hdl.handle.net/21.15107/rcub_smile_2206 .