TY  - JOUR
AU  - Roganović, Jelena
AU  - Radenković, Miroslav
AU  - Tanić, Nikola
AU  - Tanić, Nasta
AU  - Petrović, Nina
AU  - Stojić, Dragica
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1625
AB  - The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M-3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N-G-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M-3 receptor level.
PB  - Wiley, Hoboken
T2  - European Journal of Oral Sciences
T1  - Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit
VL  - 119
IS  - 5
SP  - 352
EP  - 360
DO  - 10.1111/j.1600-0722.2011.00851.x
ER  - 

@article{
author = "Roganović, Jelena and Radenković, Miroslav and Tanić, Nikola and Tanić, Nasta and Petrović, Nina and Stojić, Dragica",
year = "2011",
abstract = "The aim of this study was to assess the effect of type 1 diabetes mellitus (induced by a single intravenous injection of 100 mg kg(-1) of alloxan) on acetylcholine (ACh)-induced relaxation in isolated rabbit parotid gland feeding artery. Isometric force measurements and quantification of inducible nitric oxide synthase (iNOS) mRNA by real-time RT-PCR were made in parotid artery rings from diabetic and control rabbits. Acetylcholine induced concentration- and endothelium-dependent vasorelaxation that was significantly decreased in parotid artery rings from diabetic rabbits. Schild analysis of the ACh vasorelaxant effect, in the presence of selective muscarinic receptor antagonists, revealed involvement of the M-3 receptor subtype in parotid artery rings from both control and diabetic rabbits, with no change in antagonist affinity constants. The inhibitory effects of indomethacin, a non-selective inhibitor of cyclooxygenase, and of high potassium, an inhibitor of hyperpolarization, on ACh vasorelaxation were increased. The effect of N-G-nitro-L-arginine, a non-selective inhibitor of NOS, was decreased in diabetes. S-methylisothiourea, a selective inhibitor of iNOS, significantly reduced ACh vasorelaxation only in parotid artery rings from diabetic rabbits. Also, up-regulation of iNOS mRNA expression was detected in parotid artery rings from diabetic rabbits. These results suggest that in parotid artery rings from diabetic rabbits, impaired endothelium-dependent vasorelaxation to ACh appears to be caused by the loss of a nitric oxide-mediated component and increased iNOS expression, and is unlikely to be caused by a change at the M-3 receptor level.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Oral Sciences",
title = "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit",
volume = "119",
number = "5",
pages = "352-360",
doi = "10.1111/j.1600-0722.2011.00851.x"
}

Roganović, J., Radenković, M., Tanić, N., Tanić, N., Petrović, N.,& Stojić, D.. (2011). Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences
Wiley, Hoboken., 119(5), 352-360.
https://doi.org/10.1111/j.1600-0722.2011.00851.x

Roganović J, Radenković M, Tanić N, Tanić N, Petrović N, Stojić D. Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit. in European Journal of Oral Sciences. 2011;119(5):352-360.
doi:10.1111/j.1600-0722.2011.00851.x .

Roganović, Jelena, Radenković, Miroslav, Tanić, Nikola, Tanić, Nasta, Petrović, Nina, Stojić, Dragica, "Impairment of acetylcholine-mediated endothelium-dependent relaxation in isolated parotid artery of the alloxan-induced diabetic rabbit" in European Journal of Oral Sciences, 119, no. 5 (2011):352-360,
https://doi.org/10.1111/j.1600-0722.2011.00851.x . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Radenković, Miroslav
AU  - Stojić, Dragica
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1592
AB  - Objectives: The influence of carotid artery occlusion (10, 30 and 60 min) on regulatory mechanisms implicated in the vasorelaxant responses of isolated glandular branch of rabbit facial artery to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) was examined. Design: In organ bath studies with arterial rings precontracted with phenylephrine (1 mu M), before and after carotid artery occlusion, changes in isometric tension were recorded. Results: Endothelium-dependent vasorelaxation by ACh and endothelium-independent vasorelaxation by VIP were significantly reduced, started from 30 and 10 mm of carotid occlusion, respectively. Inhibitory effect of indomethacin on ACh vasorelaxation was enhanced whilst effect of N-G-nitro-L-arginine reduced, started from 30 min of carotid occlusion. Sodium nitroprusside-induced vasorelaxation was not changed after carotid occlusion. Inhibition of VIP vasorelaxation by L-N-omega-nitroarginine-2,4-L-diaminobutyric-amide, was reduced, started from 30 min of carotid occlusion. Forskolin enhanced VIP-induced vasorelaxation in control rings but this effect was reduced started from 30 min of occlusion. In the presence of VIP, vasorelaxant effect of ACh was increased; the increase was reduced, started from 10 min of carotid occlusion. Conclusions: The present investigation provides evidence for the decreased responsiveness to both, ACh-endothelium-dependent and VIP-endothelium-independent vasorelaxation in rabbit facial artery after carotid occlusion. In addition, the data suggest that ischaemia alters contribution of endothelial nitric oxide (eNO) and prostaglandin to ACh, and vascular smooth muscle's cAMP and neuronal NO to VIP vasorelaxant effects.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - ACh- and VIP-induced vasorelaxation in rabbit facial artery after carotid artery occlusion
VL  - 55
IS  - 5
SP  - 333
EP  - 342
DO  - 10.1016/j.archoralbio.2010.03.006
ER  - 

@article{
author = "Roganović, Jelena and Radenković, Miroslav and Stojić, Dragica",
year = "2010",
abstract = "Objectives: The influence of carotid artery occlusion (10, 30 and 60 min) on regulatory mechanisms implicated in the vasorelaxant responses of isolated glandular branch of rabbit facial artery to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) was examined. Design: In organ bath studies with arterial rings precontracted with phenylephrine (1 mu M), before and after carotid artery occlusion, changes in isometric tension were recorded. Results: Endothelium-dependent vasorelaxation by ACh and endothelium-independent vasorelaxation by VIP were significantly reduced, started from 30 and 10 mm of carotid occlusion, respectively. Inhibitory effect of indomethacin on ACh vasorelaxation was enhanced whilst effect of N-G-nitro-L-arginine reduced, started from 30 min of carotid occlusion. Sodium nitroprusside-induced vasorelaxation was not changed after carotid occlusion. Inhibition of VIP vasorelaxation by L-N-omega-nitroarginine-2,4-L-diaminobutyric-amide, was reduced, started from 30 min of carotid occlusion. Forskolin enhanced VIP-induced vasorelaxation in control rings but this effect was reduced started from 30 min of occlusion. In the presence of VIP, vasorelaxant effect of ACh was increased; the increase was reduced, started from 10 min of carotid occlusion. Conclusions: The present investigation provides evidence for the decreased responsiveness to both, ACh-endothelium-dependent and VIP-endothelium-independent vasorelaxation in rabbit facial artery after carotid occlusion. In addition, the data suggest that ischaemia alters contribution of endothelial nitric oxide (eNO) and prostaglandin to ACh, and vascular smooth muscle's cAMP and neuronal NO to VIP vasorelaxant effects.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "ACh- and VIP-induced vasorelaxation in rabbit facial artery after carotid artery occlusion",
volume = "55",
number = "5",
pages = "333-342",
doi = "10.1016/j.archoralbio.2010.03.006"
}

Roganović, J., Radenković, M.,& Stojić, D.. (2010). ACh- and VIP-induced vasorelaxation in rabbit facial artery after carotid artery occlusion. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 55(5), 333-342.
https://doi.org/10.1016/j.archoralbio.2010.03.006

Roganović J, Radenković M, Stojić D. ACh- and VIP-induced vasorelaxation in rabbit facial artery after carotid artery occlusion. in Archives of Oral Biology. 2010;55(5):333-342.
doi:10.1016/j.archoralbio.2010.03.006 .

Roganović, Jelena, Radenković, Miroslav, Stojić, Dragica, "ACh- and VIP-induced vasorelaxation in rabbit facial artery after carotid artery occlusion" in Archives of Oral Biology, 55, no. 5 (2010):333-342,
https://doi.org/10.1016/j.archoralbio.2010.03.006 . .

TY  - JOUR
AU  - Pešić, Srđan
AU  - Radenković, Miroslav
AU  - Popović-Roganović, Jelena
AU  - Pešić, Zoran
AU  - Grbović, Leosava
AU  - Stojić, Dragica
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2589
AB  - Endothelial vasodilatory substances may play a central role in the local regulation of vascular tone. We hypothesized that these substances can mediate endothelium-dependent vasodilatory responses to acetylcholine (ACh) and vasoactive intestinal peptide (VIP) in the human submandibular artery. We evaluated the contributions of endothelial vasodilatory substances to vessel relaxation in response to ACh and VIP, using different inhibitors of endothelial vasodilation, the nitric oxide synthase inhibitor, the cyclo-oxygenase inhibitor, indomethacin, the potassium channel blocker, and 4-aminopyridine. ACh and VIP caused an endothelium- and concentration-dependent relaxation in this artery. ACh relaxation was completely blocked after the concomitant addition of N(G)-nitro-L-arginine and indomethacin. The vasorelaxant effect of ACh was not influenced by 4-aminopyridine. VIP relaxation was almost completely abolished by 4-aminopyridine, and was partly inhibited by N(G)-nitro-L-arginine, but was not affected by indomethacin. Thus, in the human submandibular artery, ACh and VIP produced endothelium-dependent vasodilation with different underlying mechanisms: release of nitric oxide (NO) and cyclo-oxygenase products for ACh, and release of NO and endothelium-derived hyperpolarizing factor for VIP.
PB  - Sage Publications Inc.
T2  - Journal of Dental Research
T1  - Responses of the human submandibular artery to ACh and VIP
VL  - 86
IS  - 6
SP  - 565
EP  - 570
DO  - 10.1177/154405910708600615
ER  - 

@article{
author = "Pešić, Srđan and Radenković, Miroslav and Popović-Roganović, Jelena and Pešić, Zoran and Grbović, Leosava and Stojić, Dragica",
year = "2007",
abstract = "Endothelial vasodilatory substances may play a central role in the local regulation of vascular tone. We hypothesized that these substances can mediate endothelium-dependent vasodilatory responses to acetylcholine (ACh) and vasoactive intestinal peptide (VIP) in the human submandibular artery. We evaluated the contributions of endothelial vasodilatory substances to vessel relaxation in response to ACh and VIP, using different inhibitors of endothelial vasodilation, the nitric oxide synthase inhibitor, the cyclo-oxygenase inhibitor, indomethacin, the potassium channel blocker, and 4-aminopyridine. ACh and VIP caused an endothelium- and concentration-dependent relaxation in this artery. ACh relaxation was completely blocked after the concomitant addition of N(G)-nitro-L-arginine and indomethacin. The vasorelaxant effect of ACh was not influenced by 4-aminopyridine. VIP relaxation was almost completely abolished by 4-aminopyridine, and was partly inhibited by N(G)-nitro-L-arginine, but was not affected by indomethacin. Thus, in the human submandibular artery, ACh and VIP produced endothelium-dependent vasodilation with different underlying mechanisms: release of nitric oxide (NO) and cyclo-oxygenase products for ACh, and release of NO and endothelium-derived hyperpolarizing factor for VIP.",
publisher = "Sage Publications Inc.",
journal = "Journal of Dental Research",
title = "Responses of the human submandibular artery to ACh and VIP",
volume = "86",
number = "6",
pages = "565-570",
doi = "10.1177/154405910708600615"
}

Pešić, S., Radenković, M., Popović-Roganović, J., Pešić, Z., Grbović, L.,& Stojić, D.. (2007). Responses of the human submandibular artery to ACh and VIP. in Journal of Dental Research
Sage Publications Inc.., 86(6), 565-570.
https://doi.org/10.1177/154405910708600615

Pešić S, Radenković M, Popović-Roganović J, Pešić Z, Grbović L, Stojić D. Responses of the human submandibular artery to ACh and VIP. in Journal of Dental Research. 2007;86(6):565-570.
doi:10.1177/154405910708600615 .

Pešić, Srđan, Radenković, Miroslav, Popović-Roganović, Jelena, Pešić, Zoran, Grbović, Leosava, Stojić, Dragica, "Responses of the human submandibular artery to ACh and VIP" in Journal of Dental Research, 86, no. 6 (2007):565-570,
https://doi.org/10.1177/154405910708600615 . .

TY  - JOUR
AU  - Stojić, D.
AU  - Radenković, Miroslav
AU  - Kršljak, Elena
AU  - Popović, J
AU  - Pesić, S
AU  - Grbović, L
PY  - 2003
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1188
AB  - The aim was to examine the influence of the endothelium on acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) functional responses in the isolated glandular branch of rabbit facial artery precontracted with phenylephrine as well as the potential contribution of nitric oxide (NO) and prostanoids in the ACh- and VIP-induced effects. Acetylcholine caused endothelium-dependent and VIP endothelium-independent relaxations of facial artery. The effect of ACh was partly inhibited by N-G -monomethyl-L-arginine (L-NMMA, a non-selective NO synthase inhibitor) or by indomethacin (a cyclooxygenase inhibitor) while being completely blocked after concomitant addition of L-NMMA and indomethacin. The relaxation of the facial artery caused by ACh was unaffected by 65 mm KCl. The VIP-induced vasodilation was potentiated by forskolin (an adenylate cyclase stimulator) and partly reduced by L-NMMA or S-methyl-L-thiocitrulline (L-SMTC, a neuronal NO synthase inhibitor), whereas it was unaffected by indomethacin. These results suggest that ACh effects on the rabbit facial artery are mediated through release of endothelium-derived NO and cyclooxygenase products, while the effect of VIP is most probably mediated by an increase of cyclic adenosine 3',5'-monophosphate (cAMP) in vascular smooth muscles and by VIP-induced release of NO from perivascular nerve fibers.
PB  - Wiley, Hoboken
T2  - European Journal of Oral Sciences
T1  - Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery
VL  - 111
IS  - 2
SP  - 137
EP  - 143
DO  - 10.1034/j.1600-0722.2003.00021.x
ER  - 

@article{
author = "Stojić, D. and Radenković, Miroslav and Kršljak, Elena and Popović, J and Pesić, S and Grbović, L",
year = "2003",
abstract = "The aim was to examine the influence of the endothelium on acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) functional responses in the isolated glandular branch of rabbit facial artery precontracted with phenylephrine as well as the potential contribution of nitric oxide (NO) and prostanoids in the ACh- and VIP-induced effects. Acetylcholine caused endothelium-dependent and VIP endothelium-independent relaxations of facial artery. The effect of ACh was partly inhibited by N-G -monomethyl-L-arginine (L-NMMA, a non-selective NO synthase inhibitor) or by indomethacin (a cyclooxygenase inhibitor) while being completely blocked after concomitant addition of L-NMMA and indomethacin. The relaxation of the facial artery caused by ACh was unaffected by 65 mm KCl. The VIP-induced vasodilation was potentiated by forskolin (an adenylate cyclase stimulator) and partly reduced by L-NMMA or S-methyl-L-thiocitrulline (L-SMTC, a neuronal NO synthase inhibitor), whereas it was unaffected by indomethacin. These results suggest that ACh effects on the rabbit facial artery are mediated through release of endothelium-derived NO and cyclooxygenase products, while the effect of VIP is most probably mediated by an increase of cyclic adenosine 3',5'-monophosphate (cAMP) in vascular smooth muscles and by VIP-induced release of NO from perivascular nerve fibers.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Oral Sciences",
title = "Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery",
volume = "111",
number = "2",
pages = "137-143",
doi = "10.1034/j.1600-0722.2003.00021.x"
}

Stojić, D., Radenković, M., Kršljak, E., Popović, J., Pesić, S.,& Grbović, L.. (2003). Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery. in European Journal of Oral Sciences
Wiley, Hoboken., 111(2), 137-143.
https://doi.org/10.1034/j.1600-0722.2003.00021.x

Stojić D, Radenković M, Kršljak E, Popović J, Pesić S, Grbović L. Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery. in European Journal of Oral Sciences. 2003;111(2):137-143.
doi:10.1034/j.1600-0722.2003.00021.x .

Stojić, D., Radenković, Miroslav, Kršljak, Elena, Popović, J, Pesić, S, Grbović, L, "Influence of the endothelium on the vasorelaxant response to acetylcholine and vasoactive intestinal polypeptide in the isolated rabbit facial artery" in European Journal of Oral Sciences, 111, no. 2 (2003):137-143,
https://doi.org/10.1034/j.1600-0722.2003.00021.x . .