TY  - JOUR
AU  - Jovanović-Medojević, Milica
AU  - Opačić-Galić, Vanja
AU  - Geler, Katarina
AU  - Pavlica, Dušan
PY  - 2023
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3273
AB  - Background/Aim. There is no standard protocol for cleaning and disinfection of used endodontic instruments before their sterilization and reuse. The aim of this study was to determine the efficiency of the different methods of removing biological debris from different types of used hand stainless steel endodontic instruments. Methods. A total of 120 hand stainless steel endodontic instruments: KerrTM reamers, KerrTM files, and HedströmTM files, each forty ISO 25, used for root canal treatment on extracted teeth, were analyzed. The used instruments were divided into four groups based on different decontamination protocols. The evaluation of the efficiency of the cleaning methods was based on the evaluation of the amount of stained organic residues on the instruments (Van Gieson staining). Samples were analyzed by stereomicroscopy (x40). Statistical analysis was performed using the Mann-Whitney U test for the KerrTM reamers and HedströmTM files, while the One-Way ANOVA/Bonferroni test was used for the KerrTM files, at a significance level of 5% (a = 0.05). Results. Residual biological debris was observed on 93.3% of all the samples taken. The thermal disinfectant cleaning method showed the lowest contamination values for all types of instruments. The method of mechanical cleaning showed that the mean value of maximum biologic contamination (MBC) was 58.5% for the KerrTM reamers and 56.2% for KerrTM files, while for HedströmTM files, the highest MBC (50.2%) was shown by the ultrasonic method of cleaning. Conclusion. The use of a thermal disinfectant was the most efficient cleaning method for all three types of hand endodontic instruments.
AB  - Uvod/Cilj. Ne postoji standard za čišćenje i dezinfekciju upotrebljenih endodontskih instrumenata pre njihove sterilizacije i ponovnog korišćenja. Cilj rada bio je da se ustanovi efikasnost različitih metoda uklanjanja ostataka biološkog materijala sa radnih površina upotrebljenih ručnih endodontskih instrumenata od nerđajućeg čelika. Metode. Analizirano je ukupno 120 ručnih endodontskih instrumenata od nerđajućeg čelika: po četrdeset KerrTM proširivača, KerrTM turpija i HedströmTM turpija ISO 25, upotrebljenih za obradu kanala korena na ekstrahovanim zubima. Na osnovu protokola koji je korišćen za dekontaminaciju instrumenata, upotrebljeni instrumenti su podeljeni u četiri grupe. Procena efikasnosti korišćenih metoda čišćenja zasnovana je na proceni količine prebojenih organskih ostataka na instrumentima (bojenje po Van Gieson-u). Uzorci su analizirani stereomikroskopijom (x40). Statistička analiza dobijenih rezultata izvršena je MannWhitney U testom za KerrTM proširivače i HedströmTM turpije dok je za KerrTM turpije korišćen One-Way ANOVA/Bonferoni test, na nivou pouzdanosti od 5% (a = 0,05). Rezultati. Prisustvo rezidualnih ostataka biološkog materijala uočeno je na 93,3% svih analiziranih instrumenata. Metoda čišćenja toplotnim dezinfikatorom pokazala je najniže vrednosti kontaminacije za sve tipove instrumenata. Metoda mehaničkog čišćenja pokazala je da je srednja vrednost parametra maksimum biološke kontaminacije (MBK) bila 58,5% za KerrTM proširivače i 56,2% za KerrTM turpije, dok je za HedströmTM turpije najviša vrednost MBK (50,2%) bila posle ultrazvučne metode čišćenja instrumenata. Zaključak. Primena toplotnog dezinfikatora je najefikasnija metoda čišćenja za sva tri tipa ručnih endodontskih instrumenata.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments
T1  - Upoređivanje efikasnosti protokola čišćenja i dezinfekcije ručnih endodontskih instrumenata
VL  - 80
IS  - 7
SP  - 596
EP  - 603
DO  - 10.2298/VSP220913103J
ER  - 

@article{
author = "Jovanović-Medojević, Milica and Opačić-Galić, Vanja and Geler, Katarina and Pavlica, Dušan",
year = "2023",
abstract = "Background/Aim. There is no standard protocol for cleaning and disinfection of used endodontic instruments before their sterilization and reuse. The aim of this study was to determine the efficiency of the different methods of removing biological debris from different types of used hand stainless steel endodontic instruments. Methods. A total of 120 hand stainless steel endodontic instruments: KerrTM reamers, KerrTM files, and HedströmTM files, each forty ISO 25, used for root canal treatment on extracted teeth, were analyzed. The used instruments were divided into four groups based on different decontamination protocols. The evaluation of the efficiency of the cleaning methods was based on the evaluation of the amount of stained organic residues on the instruments (Van Gieson staining). Samples were analyzed by stereomicroscopy (x40). Statistical analysis was performed using the Mann-Whitney U test for the KerrTM reamers and HedströmTM files, while the One-Way ANOVA/Bonferroni test was used for the KerrTM files, at a significance level of 5% (a = 0.05). Results. Residual biological debris was observed on 93.3% of all the samples taken. The thermal disinfectant cleaning method showed the lowest contamination values for all types of instruments. The method of mechanical cleaning showed that the mean value of maximum biologic contamination (MBC) was 58.5% for the KerrTM reamers and 56.2% for KerrTM files, while for HedströmTM files, the highest MBC (50.2%) was shown by the ultrasonic method of cleaning. Conclusion. The use of a thermal disinfectant was the most efficient cleaning method for all three types of hand endodontic instruments., Uvod/Cilj. Ne postoji standard za čišćenje i dezinfekciju upotrebljenih endodontskih instrumenata pre njihove sterilizacije i ponovnog korišćenja. Cilj rada bio je da se ustanovi efikasnost različitih metoda uklanjanja ostataka biološkog materijala sa radnih površina upotrebljenih ručnih endodontskih instrumenata od nerđajućeg čelika. Metode. Analizirano je ukupno 120 ručnih endodontskih instrumenata od nerđajućeg čelika: po četrdeset KerrTM proširivača, KerrTM turpija i HedströmTM turpija ISO 25, upotrebljenih za obradu kanala korena na ekstrahovanim zubima. Na osnovu protokola koji je korišćen za dekontaminaciju instrumenata, upotrebljeni instrumenti su podeljeni u četiri grupe. Procena efikasnosti korišćenih metoda čišćenja zasnovana je na proceni količine prebojenih organskih ostataka na instrumentima (bojenje po Van Gieson-u). Uzorci su analizirani stereomikroskopijom (x40). Statistička analiza dobijenih rezultata izvršena je MannWhitney U testom za KerrTM proširivače i HedströmTM turpije dok je za KerrTM turpije korišćen One-Way ANOVA/Bonferoni test, na nivou pouzdanosti od 5% (a = 0,05). Rezultati. Prisustvo rezidualnih ostataka biološkog materijala uočeno je na 93,3% svih analiziranih instrumenata. Metoda čišćenja toplotnim dezinfikatorom pokazala je najniže vrednosti kontaminacije za sve tipove instrumenata. Metoda mehaničkog čišćenja pokazala je da je srednja vrednost parametra maksimum biološke kontaminacije (MBK) bila 58,5% za KerrTM proširivače i 56,2% za KerrTM turpije, dok je za HedströmTM turpije najviša vrednost MBK (50,2%) bila posle ultrazvučne metode čišćenja instrumenata. Zaključak. Primena toplotnog dezinfikatora je najefikasnija metoda čišćenja za sva tri tipa ručnih endodontskih instrumenata.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments, Upoređivanje efikasnosti protokola čišćenja i dezinfekcije ručnih endodontskih instrumenata",
volume = "80",
number = "7",
pages = "596-603",
doi = "10.2298/VSP220913103J"
}

Jovanović-Medojević, M., Opačić-Galić, V., Geler, K.,& Pavlica, D.. (2023). Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 80(7), 596-603.
https://doi.org/10.2298/VSP220913103J

Jovanović-Medojević M, Opačić-Galić V, Geler K, Pavlica D. Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments. in Vojnosanitetski pregled. 2023;80(7):596-603.
doi:10.2298/VSP220913103J .

Jovanović-Medojević, Milica, Opačić-Galić, Vanja, Geler, Katarina, Pavlica, Dušan, "Comparison of the efficiency of cleaning and disinfection protocols for hand endodontic instruments" in Vojnosanitetski pregled, 80, no. 7 (2023):596-603,
https://doi.org/10.2298/VSP220913103J . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Radunović, Milena
AU  - Barać, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2463
AB  - Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects
VL  - 14
IS  - 1
DO  - 10.1371/journal.pone.0210527
ER  - 

@article{
author = "Matić-Petrović, Sanja and Radunović, Milena and Barać, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2019",
abstract = "Objectives The aim of this cross-sectional observational study was to compare the prevalence of different oral Candida spp. in patients with Type 2 Diabetes and chronic periodontitis in two oral sites: dorsal surface of the tongue and subgingival area. In order to determine subgingival areas as potential reservoirs of yeasts, this study aimed to find differences in the yeasts' detection between the dorsum of the tongue, as the oral site most commonly inhabited with microorganisms, and subgingival samples. Additionally, potential predictors for the yeasts prevalence were determined. Material and methods Subjects (N = 146) were divided into four groups: group A-healthy individuals without periodontitis, group B-healthy individuals with chronic periodontitis, group C-Type 2 Diabetes patients with good glycoregulation and Chronic periodontitis and group D-Type 2 Diabetes patients with poor glycoregulation and Chronic periodontitis. Samples were obtained from the tongue by swabbing. Subgingival plaque samples were taken by paper points and periodontal curette. Isolation and identification of different Candida spp. was done using ChromAgar medium. In addition, germ-tube production and carbohydrate assimilation tests were performed. Results The prevalence of Candida spp. was higher in diabetics with poor glycoregulation. The most frequently isolated species was Candida albicans followed by Candida glabrata and Candida tropicalis. In 15.6% of cases, Candida spp. was present in the subgingival area while absent on the tongue. Multivariate regression model showed that HbA1c was Candida spp. predictor for both locations. Conclusions Our results confirmed that there are Candida spp. carriers among subjects with clinically healthy oral mucosa. Also, this study identified subgingival areas as potential reservoirs of these pathogenic species. Glycoregulation has been recognized as a positive predictor factor of Candida spp.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects",
volume = "14",
number = "1",
doi = "10.1371/journal.pone.0210527"
}

Matić-Petrović, S., Radunović, M., Barać, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2019). Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One
Public Library Science, San Francisco., 14(1).
https://doi.org/10.1371/journal.pone.0210527

Matić-Petrović S, Radunović M, Barać M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects. in PLoS One. 2019;14(1).
doi:10.1371/journal.pone.0210527 .

Matić-Petrović, Sanja, Radunović, Milena, Barać, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival areas as potential reservoirs of different Candida spp in type 2 diabetes patients and healthy subjects" in PLoS One, 14, no. 1 (2019),
https://doi.org/10.1371/journal.pone.0210527 . .

TY  - JOUR
AU  - Matić-Petrović, Sanja
AU  - Đorđević, Mihajlo
AU  - Radunović, Milena
AU  - Živanović, Tanja
AU  - Pavlica, Dušan
AU  - Pucar, Ana
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2406
AB  - Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - Geographic tongue: Does Candida play a role in its pathogenesis
VL  - 23
IS  - 3
SP  - 152
EP  - 156
DO  - 10.2478/bjdm-2019-0027
ER  - 

@article{
author = "Matić-Petrović, Sanja and Đorđević, Mihajlo and Radunović, Milena and Živanović, Tanja and Pavlica, Dušan and Pucar, Ana",
year = "2019",
abstract = "Background/Aim: Geographic tongue (GT), a benign self-limiting condition (inflammation) is commonly seen in practice. Although quite easy for clinical diagnosis, in clinical practice it is commonly misdiagnosed and treated as Candida infection. The main aim of this study was to compare the prevalence of Candida spp. on tongue in patients with GT and subjects with clinically healthy oral mucosa. Additional aims were to evaluate subjective symptoms, uncontrolled usage of prescribed or non-prescribed antifungal topical medications and the presence of the cancerophobia or other health concerns in patients with GT. Material and Methods: A total of 70 subjects were divided into two groups: Group B - patients with diagnosed Geographic tongue and group A - aged and gender matched controls with clinically health oral mucosa. Anamnestic charts designed for this study included information about symptoms (measured by Visual Analogue Scale), previous knowledge or fear about presence of GT and received therapy for this condition. Detection of Candida spp. from tongue was done using sterile cotton swab and Sabouraud dextrose agar. Results: At the time of this study, 18 (52.94%) of subjects with GT were aware of having this condition and even 12 of them (66.7%) used topical antifungal drugs prescribed by physician. Also, 66.7% of them experienced fear about the presence of GT at some moment. Detection of Candida spp. was similarly distributed in both groups (22.2% in group A and 17.6% in group B). Conclusions: Geographic tongue has frequently been treated by topical antifungal drugs. In this study, GT was not associated with presence of Candida spp.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "Geographic tongue: Does Candida play a role in its pathogenesis",
volume = "23",
number = "3",
pages = "152-156",
doi = "10.2478/bjdm-2019-0027"
}

Matić-Petrović, S., Đorđević, M., Radunović, M., Živanović, T., Pavlica, D.,& Pucar, A.. (2019). Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 23(3), 152-156.
https://doi.org/10.2478/bjdm-2019-0027

Matić-Petrović S, Đorđević M, Radunović M, Živanović T, Pavlica D, Pucar A. Geographic tongue: Does Candida play a role in its pathogenesis. in Balkan Journal of Dental Medicine. 2019;23(3):152-156.
doi:10.2478/bjdm-2019-0027 .

Matić-Petrović, Sanja, Đorđević, Mihajlo, Radunović, Milena, Živanović, Tanja, Pavlica, Dušan, Pucar, Ana, "Geographic tongue: Does Candida play a role in its pathogenesis" in Balkan Journal of Dental Medicine, 23, no. 3 (2019):152-156,
https://doi.org/10.2478/bjdm-2019-0027 . .

TY  - CONF
AU  - Radunović, Milena
AU  - Matić-Petrović, Sanja
AU  - Cimbaljević, Milena
AU  - Kuzmanović-Pfićer, Jovana
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
AU  - Pucar, Ana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2295
PB  - Oxford Univ Press, Oxford
C3  - Medical Mycology
T1  - Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics
VL  - 56
SP  - S108
EP  - S108
UR  - https://hdl.handle.net/21.15107/rcub_smile_2295
ER  - 

@conference{
author = "Radunović, Milena and Matić-Petrović, Sanja and Cimbaljević, Milena and Kuzmanović-Pfićer, Jovana and Pavlica, Dušan and Arsić-Arsenijević, Valentina and Pucar, Ana",
year = "2018",
publisher = "Oxford Univ Press, Oxford",
journal = "Medical Mycology",
title = "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics",
volume = "56",
pages = "S108-S108",
url = "https://hdl.handle.net/21.15107/rcub_smile_2295"
}

Radunović, M., Matić-Petrović, S., Cimbaljević, M., Kuzmanović-Pfićer, J., Pavlica, D., Arsić-Arsenijević, V.,& Pucar, A.. (2018). Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology
Oxford Univ Press, Oxford., 56, S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295

Radunović M, Matić-Petrović S, Cimbaljević M, Kuzmanović-Pfićer J, Pavlica D, Arsić-Arsenijević V, Pucar A. Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics. in Medical Mycology. 2018;56:S108-S108.
https://hdl.handle.net/21.15107/rcub_smile_2295 .

Radunović, Milena, Matić-Petrović, Sanja, Cimbaljević, Milena, Kuzmanović-Pfićer, Jovana, Pavlica, Dušan, Arsić-Arsenijević, Valentina, Pucar, Ana, "Subgingival sites and oral cavity as reservoirs for Candida spp. in diabetics and non-diabetics" in Medical Mycology, 56 (2018):S108-S108,
https://hdl.handle.net/21.15107/rcub_smile_2295 .

TY  - CONF
AU  - Perić, M.
AU  - Pucar, Ana
AU  - Živković, Rade
AU  - Radunović, Milena
AU  - Pavlica, Dušan
AU  - Arsić-Arsenijević, Valentina
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1927
PB  - Wiley-Blackwell, Hoboken
C3  - Oral Diseases
T1  - Evaluation of different sampling methods in patients with denture stomatitis
VL  - 20
SP  - 22
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_smile_1927
ER  - 

@conference{
author = "Perić, M. and Pucar, Ana and Živković, Rade and Radunović, Milena and Pavlica, Dušan and Arsić-Arsenijević, Valentina",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Oral Diseases",
title = "Evaluation of different sampling methods in patients with denture stomatitis",
volume = "20",
pages = "22-22",
url = "https://hdl.handle.net/21.15107/rcub_smile_1927"
}

Perić, M., Pucar, A., Živković, R., Radunović, M., Pavlica, D.,& Arsić-Arsenijević, V.. (2014). Evaluation of different sampling methods in patients with denture stomatitis. in Oral Diseases
Wiley-Blackwell, Hoboken., 20, 22-22.
https://hdl.handle.net/21.15107/rcub_smile_1927

Perić M, Pucar A, Živković R, Radunović M, Pavlica D, Arsić-Arsenijević V. Evaluation of different sampling methods in patients with denture stomatitis. in Oral Diseases. 2014;20:22-22.
https://hdl.handle.net/21.15107/rcub_smile_1927 .

Perić, M., Pucar, Ana, Živković, Rade, Radunović, Milena, Pavlica, Dušan, Arsić-Arsenijević, Valentina, "Evaluation of different sampling methods in patients with denture stomatitis" in Oral Diseases, 20 (2014):22-22,
https://hdl.handle.net/21.15107/rcub_smile_1927 .

TY  - JOUR
AU  - Lazović, Saša
AU  - Puač, Nevena
AU  - Miletić, Maja
AU  - Pavlica, Dušan
AU  - Jovanović, Milena
AU  - Bugarski, Diana
AU  - Mojsilović, Slavko
AU  - Maletić, Dejan
AU  - Malović, Gordana
AU  - Milenković, Pavle
AU  - Petrović, Zoran Lj.
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1559
AB  - In this paper, we study the application of a plasma needle to induce necrosis in planktonic samples containing a single breed of bacteria. Two different types of bacteria, Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), were covered in this study. In all experiments with bacteria, the samples were liquid suspensions of several different concentrations of bacteria prepared according to the McFarland standard. The second system studied in this paper was human peripheral blood mesenchymal stem cells (hPB-MSC). In the case of hPB-MSC, two sets of experiments were performed: when cells were covered with a certain amount of liquid (indirect) and when the cell sample was in direct contact with the plasma. Most importantly, the study is made with the aim to see the effects when the living cells are in a liquid medium, which normally acts as protection against the many agents that may be released by plasmas. It was found that a good effect may be expected for a wide range of initial cell densities and operating conditions causing destruction of several orders of magnitude even under the protection of a liquid. It was established independently that a temperature increase could not affect the cells under the conditions of our experiment, so the effect could those hPB-MSC that were not protected by a liquid, gas flow proved to produce a considerable effect, presumably due to poor adhesion of the cells, but in a liquid the effect was only due to the plasma. Further optimization of the operation may be attempted, opening up the possibility of localized in vivo sterilization.
PB  - Iop Publishing Ltd, Bristol
T2  - New Journal of Physics
T1  - The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells
VL  - 12
DO  - 10.1088/1367-2630/12/8/083037
ER  - 

@article{
author = "Lazović, Saša and Puač, Nevena and Miletić, Maja and Pavlica, Dušan and Jovanović, Milena and Bugarski, Diana and Mojsilović, Slavko and Maletić, Dejan and Malović, Gordana and Milenković, Pavle and Petrović, Zoran Lj.",
year = "2010",
abstract = "In this paper, we study the application of a plasma needle to induce necrosis in planktonic samples containing a single breed of bacteria. Two different types of bacteria, Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922), were covered in this study. In all experiments with bacteria, the samples were liquid suspensions of several different concentrations of bacteria prepared according to the McFarland standard. The second system studied in this paper was human peripheral blood mesenchymal stem cells (hPB-MSC). In the case of hPB-MSC, two sets of experiments were performed: when cells were covered with a certain amount of liquid (indirect) and when the cell sample was in direct contact with the plasma. Most importantly, the study is made with the aim to see the effects when the living cells are in a liquid medium, which normally acts as protection against the many agents that may be released by plasmas. It was found that a good effect may be expected for a wide range of initial cell densities and operating conditions causing destruction of several orders of magnitude even under the protection of a liquid. It was established independently that a temperature increase could not affect the cells under the conditions of our experiment, so the effect could those hPB-MSC that were not protected by a liquid, gas flow proved to produce a considerable effect, presumably due to poor adhesion of the cells, but in a liquid the effect was only due to the plasma. Further optimization of the operation may be attempted, opening up the possibility of localized in vivo sterilization.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "New Journal of Physics",
title = "The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells",
volume = "12",
doi = "10.1088/1367-2630/12/8/083037"
}

Lazović, S., Puač, N., Miletić, M., Pavlica, D., Jovanović, M., Bugarski, D., Mojsilović, S., Maletić, D., Malović, G., Milenković, P.,& Petrović, Z. Lj.. (2010). The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells. in New Journal of Physics
Iop Publishing Ltd, Bristol., 12.
https://doi.org/10.1088/1367-2630/12/8/083037

Lazović S, Puač N, Miletić M, Pavlica D, Jovanović M, Bugarski D, Mojsilović S, Maletić D, Malović G, Milenković P, Petrović ZL. The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells. in New Journal of Physics. 2010;12.
doi:10.1088/1367-2630/12/8/083037 .

Lazović, Saša, Puač, Nevena, Miletić, Maja, Pavlica, Dušan, Jovanović, Milena, Bugarski, Diana, Mojsilović, Slavko, Maletić, Dejan, Malović, Gordana, Milenković, Pavle, Petrović, Zoran Lj., "The effect of a plasma needle on bacteria in planktonic samples and on peripheral blood mesenchymal stem cells" in New Journal of Physics, 12 (2010),
https://doi.org/10.1088/1367-2630/12/8/083037 . .

TY  - JOUR
AU  - Rakić, Mia
AU  - Zelić, Ksenija
AU  - Pavlica, Dušan
AU  - Hadži-Mihailović, Miloš
AU  - Milašin, Jelena
AU  - Miličić, Biljana
AU  - Nikolić, Nebojša
AU  - Stamatović, Novak
AU  - Matić, Smiljana
AU  - Aleksić, Zoran
AU  - Janković, Saša
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1543
AB  - Background/Aim. Periodontitis is a chronic inflammatory disease of periodontal tissues with consequential is bone loss as a result of host immunological reactions caused by periopathogens. The aim of the study was to investigate if there is a correlation between clinical parameters and the presence of two most aggressive periopathogens (Aggregatibacter actinomycetemcomitans - Aa and Porphyromonas gingivalis - Pg) in patients with progressive periodontal lesions. Methods. A total of 34 systemic healthy people, 23 to 70 years old, were included in the study. The patients were clinically and radiologically examined, and after that, the representative pocket with greatest pocket depth was chosen and the sample was collected from that place. The measured clinic parameters were: gingival index, index of gingival bleeding, pocket depth and plaque indices. The multiplex Polymerase Chain Reaction (PCR) method was used for detection of periopathogens. After obtaining results, appropriate statistical tests were used to correlate the clinical and microbiological results. Results. Aa and Pg were detected in the same percentage of samples. Aa and Pg were detected in 35.29% samples alone, and in 29.41% both were detected. The values of measured clinical parameters did not show a statistical significance between the groups. In analysis of correlations among clinical parameters inside the groups, a statistical significance was found only between gingival and plaque index in the group with Aa. Conclusion. Clinical course of periodontitis in the developed stage does not differ in relation to the presence of different periopathogens as the major inductors of immunologically guided destructive processes.
AB  - Uvod/Cilj. Parodontopatija je hronično inflamatorno oboljenje parodontalnih tkiva koje za krajnji ishod ima gubitak potpornog koštanog tkiva zuba usled imunoloških reakcija izazvanih parodontopatogenim bakterijama. Cilj studije bio je korelisanje kliničkih parametara i prisustva dve najagresivnije parodontopatogene bakterije (Aggregatibacter actinomycetemcomitans - A.a. i Porphyromonas gingivalis - P.g.) kod bolesnika sa progresivnim parodontalnim lezijama. Metode. U studiju su bila uključena 34 sistemski zdrava ispitanika, starost 23-70 godina. Ispitanici su klinički i radiološki pregledani i uzorak je uziman iz reprezentativnog parodontalnog džepa sa najvećom dubinom sondiranja. Od kliničkih parametara mereni su gingivalni indeks, indeks krvarenja gingive, dubina parodontalnog džepa i indeks plaka. Prisustvo parodontopatogena dokazivano je multipleks metodom PCR (Polymerase Chain Reaction), a rezultati su korelisani sa kliničkim parametrima primenom odgovarajućih statističkih testova. Rezultati. Ista procentualna zastupljenost oba mikroorganizma dokazana je u uzorcima, naime i A.a. i P.g. bili su prisutni u po 35,39% uzoraka, a u 29,41% dokazana su oba mikroorganizma. Rezultati su korelisani po grupama formiranim u odnosu na prisustvo bakterija. Vrednosti merenih kliničkih parametara nisu se statistički značajno razlikovale u zavisnosti od prisustva parodontopatogena. Međusobne korelacije kliničkih parametara unutar grupe nisu pokazale statističku značajnost, osim korelacije gingivalnog i plak indeksa u grupi sa A.a. Zaključak. Klinički tok uznapredovale faze parodontopatije ne razlikuje se u odnosu na vrstu parodontalnih bakterija kao induktora imunološki posredovanih destruktivnih procesa.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Association between clinical parameters and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in patients with progressive periodontal lesions
T1  - Povezanost kliničkih parametara i prisustva Aggregatibacter actinomycetemcomitans i Porphyromonas gingivalis kod pacijenata sa progresivnim parodontalnim lezijama
VL  - 67
IS  - 11
SP  - 898
EP  - 902
DO  - 10.2298/VSP1011898R
ER  - 

@article{
author = "Rakić, Mia and Zelić, Ksenija and Pavlica, Dušan and Hadži-Mihailović, Miloš and Milašin, Jelena and Miličić, Biljana and Nikolić, Nebojša and Stamatović, Novak and Matić, Smiljana and Aleksić, Zoran and Janković, Saša",
year = "2010",
abstract = "Background/Aim. Periodontitis is a chronic inflammatory disease of periodontal tissues with consequential is bone loss as a result of host immunological reactions caused by periopathogens. The aim of the study was to investigate if there is a correlation between clinical parameters and the presence of two most aggressive periopathogens (Aggregatibacter actinomycetemcomitans - Aa and Porphyromonas gingivalis - Pg) in patients with progressive periodontal lesions. Methods. A total of 34 systemic healthy people, 23 to 70 years old, were included in the study. The patients were clinically and radiologically examined, and after that, the representative pocket with greatest pocket depth was chosen and the sample was collected from that place. The measured clinic parameters were: gingival index, index of gingival bleeding, pocket depth and plaque indices. The multiplex Polymerase Chain Reaction (PCR) method was used for detection of periopathogens. After obtaining results, appropriate statistical tests were used to correlate the clinical and microbiological results. Results. Aa and Pg were detected in the same percentage of samples. Aa and Pg were detected in 35.29% samples alone, and in 29.41% both were detected. The values of measured clinical parameters did not show a statistical significance between the groups. In analysis of correlations among clinical parameters inside the groups, a statistical significance was found only between gingival and plaque index in the group with Aa. Conclusion. Clinical course of periodontitis in the developed stage does not differ in relation to the presence of different periopathogens as the major inductors of immunologically guided destructive processes., Uvod/Cilj. Parodontopatija je hronično inflamatorno oboljenje parodontalnih tkiva koje za krajnji ishod ima gubitak potpornog koštanog tkiva zuba usled imunoloških reakcija izazvanih parodontopatogenim bakterijama. Cilj studije bio je korelisanje kliničkih parametara i prisustva dve najagresivnije parodontopatogene bakterije (Aggregatibacter actinomycetemcomitans - A.a. i Porphyromonas gingivalis - P.g.) kod bolesnika sa progresivnim parodontalnim lezijama. Metode. U studiju su bila uključena 34 sistemski zdrava ispitanika, starost 23-70 godina. Ispitanici su klinički i radiološki pregledani i uzorak je uziman iz reprezentativnog parodontalnog džepa sa najvećom dubinom sondiranja. Od kliničkih parametara mereni su gingivalni indeks, indeks krvarenja gingive, dubina parodontalnog džepa i indeks plaka. Prisustvo parodontopatogena dokazivano je multipleks metodom PCR (Polymerase Chain Reaction), a rezultati su korelisani sa kliničkim parametrima primenom odgovarajućih statističkih testova. Rezultati. Ista procentualna zastupljenost oba mikroorganizma dokazana je u uzorcima, naime i A.a. i P.g. bili su prisutni u po 35,39% uzoraka, a u 29,41% dokazana su oba mikroorganizma. Rezultati su korelisani po grupama formiranim u odnosu na prisustvo bakterija. Vrednosti merenih kliničkih parametara nisu se statistički značajno razlikovale u zavisnosti od prisustva parodontopatogena. Međusobne korelacije kliničkih parametara unutar grupe nisu pokazale statističku značajnost, osim korelacije gingivalnog i plak indeksa u grupi sa A.a. Zaključak. Klinički tok uznapredovale faze parodontopatije ne razlikuje se u odnosu na vrstu parodontalnih bakterija kao induktora imunološki posredovanih destruktivnih procesa.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Association between clinical parameters and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in patients with progressive periodontal lesions, Povezanost kliničkih parametara i prisustva Aggregatibacter actinomycetemcomitans i Porphyromonas gingivalis kod pacijenata sa progresivnim parodontalnim lezijama",
volume = "67",
number = "11",
pages = "898-902",
doi = "10.2298/VSP1011898R"
}

Rakić, M., Zelić, K., Pavlica, D., Hadži-Mihailović, M., Milašin, J., Miličić, B., Nikolić, N., Stamatović, N., Matić, S., Aleksić, Z.,& Janković, S.. (2010). Association between clinical parameters and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in patients with progressive periodontal lesions. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 67(11), 898-902.
https://doi.org/10.2298/VSP1011898R

Rakić M, Zelić K, Pavlica D, Hadži-Mihailović M, Milašin J, Miličić B, Nikolić N, Stamatović N, Matić S, Aleksić Z, Janković S. Association between clinical parameters and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in patients with progressive periodontal lesions. in Vojnosanitetski pregled. 2010;67(11):898-902.
doi:10.2298/VSP1011898R .

Rakić, Mia, Zelić, Ksenija, Pavlica, Dušan, Hadži-Mihailović, Miloš, Milašin, Jelena, Miličić, Biljana, Nikolić, Nebojša, Stamatović, Novak, Matić, Smiljana, Aleksić, Zoran, Janković, Saša, "Association between clinical parameters and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in patients with progressive periodontal lesions" in Vojnosanitetski pregled, 67, no. 11 (2010):898-902,
https://doi.org/10.2298/VSP1011898R . .

TY  - JOUR
AU  - Đurić, Milanko
AU  - Janković, Ljiljana
AU  - Jovanović, Tanja
AU  - Pavlica, Dušan
AU  - Brkić, Snežana
AU  - Knežević, Aleksandra
AU  - Marković, Dubravka
AU  - Milašin, Jelena
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1504
AB  - Oral reactivation of latent Herpes simplex virus (HSV) infection may easily occur in cancer patients. Virus reactivation can cause oral mucosa damage, worsen already existing lesions caused by stomatotoxic effect of cancer therapy and, whether symptomatic or asymptomatic, ample spreading and promote viral transmission. Polymerase chain reaction (PCR), cell-culture and direct immunofluorescence have been used to determine the frequency of oral HSV reactivation in 60 patients undergoing chemotherapy for different malignancies. By means of PCR, the presence of viral DNA was detected in 71.7% of patients prior to chemotherapy and in 85.0% after chemotherapy. 33.3% of patients before and 40.0% after chemotherapy were viral-culture positive, while 3.3% of patients before and 11.7% after chemotherapy were positive as shown by direct immunofluorescence. No significant difference in HSV-1 reactivation was found before and after chemotherapy. In addition, no significant difference was found when comparing HSV-1 reactivation in patients with and without mucositis. HSV-2 was not detected in any of the patients. Reactivation of latent HSV is exceptionally frequent in cancer patients. The results of this study suggest that virus reactivation occurs independently of cancer chemotherapy. The potential role of HSV reactivation in oral mucosa damage remains unclear.
PB  - Wiley, Hoboken
T2  - Journal of Oral Pathology & Medicine
T1  - Prevalence of oral herpes simplex virus reactivation in cancer patients: a comparison of different techniques of viral detection
VL  - 38
IS  - 2
SP  - 167
EP  - 173
DO  - 10.1111/j.1600-0714.2008.00684.x
ER  - 

@article{
author = "Đurić, Milanko and Janković, Ljiljana and Jovanović, Tanja and Pavlica, Dušan and Brkić, Snežana and Knežević, Aleksandra and Marković, Dubravka and Milašin, Jelena",
year = "2009",
abstract = "Oral reactivation of latent Herpes simplex virus (HSV) infection may easily occur in cancer patients. Virus reactivation can cause oral mucosa damage, worsen already existing lesions caused by stomatotoxic effect of cancer therapy and, whether symptomatic or asymptomatic, ample spreading and promote viral transmission. Polymerase chain reaction (PCR), cell-culture and direct immunofluorescence have been used to determine the frequency of oral HSV reactivation in 60 patients undergoing chemotherapy for different malignancies. By means of PCR, the presence of viral DNA was detected in 71.7% of patients prior to chemotherapy and in 85.0% after chemotherapy. 33.3% of patients before and 40.0% after chemotherapy were viral-culture positive, while 3.3% of patients before and 11.7% after chemotherapy were positive as shown by direct immunofluorescence. No significant difference in HSV-1 reactivation was found before and after chemotherapy. In addition, no significant difference was found when comparing HSV-1 reactivation in patients with and without mucositis. HSV-2 was not detected in any of the patients. Reactivation of latent HSV is exceptionally frequent in cancer patients. The results of this study suggest that virus reactivation occurs independently of cancer chemotherapy. The potential role of HSV reactivation in oral mucosa damage remains unclear.",
publisher = "Wiley, Hoboken",
journal = "Journal of Oral Pathology & Medicine",
title = "Prevalence of oral herpes simplex virus reactivation in cancer patients: a comparison of different techniques of viral detection",
volume = "38",
number = "2",
pages = "167-173",
doi = "10.1111/j.1600-0714.2008.00684.x"
}

Đurić, M., Janković, L., Jovanović, T., Pavlica, D., Brkić, S., Knežević, A., Marković, D.,& Milašin, J.. (2009). Prevalence of oral herpes simplex virus reactivation in cancer patients: a comparison of different techniques of viral detection. in Journal of Oral Pathology & Medicine
Wiley, Hoboken., 38(2), 167-173.
https://doi.org/10.1111/j.1600-0714.2008.00684.x

Đurić M, Janković L, Jovanović T, Pavlica D, Brkić S, Knežević A, Marković D, Milašin J. Prevalence of oral herpes simplex virus reactivation in cancer patients: a comparison of different techniques of viral detection. in Journal of Oral Pathology & Medicine. 2009;38(2):167-173.
doi:10.1111/j.1600-0714.2008.00684.x .

Đurić, Milanko, Janković, Ljiljana, Jovanović, Tanja, Pavlica, Dušan, Brkić, Snežana, Knežević, Aleksandra, Marković, Dubravka, Milašin, Jelena, "Prevalence of oral herpes simplex virus reactivation in cancer patients: a comparison of different techniques of viral detection" in Journal of Oral Pathology & Medicine, 38, no. 2 (2009):167-173,
https://doi.org/10.1111/j.1600-0714.2008.00684.x . .

TY  - JOUR
AU  - Milićević, Radovan
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Pavlica, Dušan
AU  - Leković, Vojislav
AU  - Milašin, Jelena
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1385
AB  - INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.
AB  - Uvod Epidemiološki podaci iz čitavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma ključnu ulogu igraju različiti rodovi Gram-negativnih bakterija, ponajviše striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u različitim uzorcima poreklom iz usne duplje pacijenata s klinički dijagnostikovanom parodontopatijom. Metod rada Kao biološki materijal u kojem je dokazivano postojanje DNK mikroorganizama korišćeni su zubni plak, tkivo zapaljene gingive i pljuvačka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lančanog umnožavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve različite bakterije. Rezultati S manjom ili većom učestalošću, u svim ispitanim uzorcima utvrđeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najčešći je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najvećem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, što je odlika hroničnog oblika parodontopatije, a u pljuvački ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapaženo u pljuvački. Zaključak Primenjeni metod PCR ima veliku osetljivost i specifičnost. Brzo i precizno otkrivanje mikroorganizama je veoma važno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i lečenje parodontopatija. U svakodnevnoj kliničkoj praksi optimalan biološki materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Identification of periodontopathogen microorganisms by PCR technique
T1  - Identifikacija parodontopatogenih mikroorganizama PCR tehnikom
VL  - 136
IS  - 9-10
SP  - 476
EP  - 480
DO  - 10.2298/SARH0810476M
ER  - 

@article{
author = "Milićević, Radovan and Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Pavlica, Dušan and Leković, Vojislav and Milašin, Jelena",
year = "2008",
abstract = "INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria., Uvod Epidemiološki podaci iz čitavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma ključnu ulogu igraju različiti rodovi Gram-negativnih bakterija, ponajviše striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u različitim uzorcima poreklom iz usne duplje pacijenata s klinički dijagnostikovanom parodontopatijom. Metod rada Kao biološki materijal u kojem je dokazivano postojanje DNK mikroorganizama korišćeni su zubni plak, tkivo zapaljene gingive i pljuvačka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lančanog umnožavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve različite bakterije. Rezultati S manjom ili većom učestalošću, u svim ispitanim uzorcima utvrđeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najčešći je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najvećem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, što je odlika hroničnog oblika parodontopatije, a u pljuvački ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapaženo u pljuvački. Zaključak Primenjeni metod PCR ima veliku osetljivost i specifičnost. Brzo i precizno otkrivanje mikroorganizama je veoma važno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i lečenje parodontopatija. U svakodnevnoj kliničkoj praksi optimalan biološki materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Identification of periodontopathogen microorganisms by PCR technique, Identifikacija parodontopatogenih mikroorganizama PCR tehnikom",
volume = "136",
number = "9-10",
pages = "476-480",
doi = "10.2298/SARH0810476M"
}

Milićević, R., Brajović, G., Nikolić-Jakoba, N., Popović, B., Pavlica, D., Leković, V.,& Milašin, J.. (2008). Identification of periodontopathogen microorganisms by PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 136(9-10), 476-480.
https://doi.org/10.2298/SARH0810476M

Milićević R, Brajović G, Nikolić-Jakoba N, Popović B, Pavlica D, Leković V, Milašin J. Identification of periodontopathogen microorganisms by PCR technique. in Srpski arhiv za celokupno lekarstvo. 2008;136(9-10):476-480.
doi:10.2298/SARH0810476M .

Milićević, Radovan, Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Pavlica, Dušan, Leković, Vojislav, Milašin, Jelena, "Identification of periodontopathogen microorganisms by PCR technique" in Srpski arhiv za celokupno lekarstvo, 136, no. 9-10 (2008):476-480,
https://doi.org/10.2298/SARH0810476M . .

TY  - JOUR
AU  - Đurić, M.
AU  - Pavlica, Dušan
AU  - Janković, L.
AU  - Milašin, Jelena
AU  - Jovanović, T.
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1344
AB  - Background The aim of this study was to confirm the presence of herpes simplex virus type 1 and 2 on the oral mucosa, in patients undergoing chemotherapy, by means of polymerase chain reaction (PCR). Methods The research was carried out on 40 patients receiving chemotherapy as treatment for different malignancies. The status of oral mucosa and viral presence were assessed in all patients at the initial examination (prior to chemotherapy), and at the control examination (two weeks after the initiation of the chemotherapeutic cycle). Results The presence of HSV-1 was detected in 28 patients (70%) prior to chemotherapy, of whom 7 (25%) manifested oral complications. The control examination showed the presence of HSV-1 in 35 patients (87.5%), of whom 23 (65.7%) presented oral mucosa changes. HSV-2 has not been detected in any of the patients.
PB  - Sage Publications Ltd, London
T2  - Scottish Medical Journal
T1  - Presence of herpes simplex virus on the oral mucosa in patients undergoing chemotherapy
VL  - 52
IS  - 1
SP  - 28
EP  - 31
DO  - 10.1258/rsmsmj.52.1.28
ER  - 

@article{
author = "Đurić, M. and Pavlica, Dušan and Janković, L. and Milašin, Jelena and Jovanović, T.",
year = "2007",
abstract = "Background The aim of this study was to confirm the presence of herpes simplex virus type 1 and 2 on the oral mucosa, in patients undergoing chemotherapy, by means of polymerase chain reaction (PCR). Methods The research was carried out on 40 patients receiving chemotherapy as treatment for different malignancies. The status of oral mucosa and viral presence were assessed in all patients at the initial examination (prior to chemotherapy), and at the control examination (two weeks after the initiation of the chemotherapeutic cycle). Results The presence of HSV-1 was detected in 28 patients (70%) prior to chemotherapy, of whom 7 (25%) manifested oral complications. The control examination showed the presence of HSV-1 in 35 patients (87.5%), of whom 23 (65.7%) presented oral mucosa changes. HSV-2 has not been detected in any of the patients.",
publisher = "Sage Publications Ltd, London",
journal = "Scottish Medical Journal",
title = "Presence of herpes simplex virus on the oral mucosa in patients undergoing chemotherapy",
volume = "52",
number = "1",
pages = "28-31",
doi = "10.1258/rsmsmj.52.1.28"
}

Đurić, M., Pavlica, D., Janković, L., Milašin, J.,& Jovanović, T.. (2007). Presence of herpes simplex virus on the oral mucosa in patients undergoing chemotherapy. in Scottish Medical Journal
Sage Publications Ltd, London., 52(1), 28-31.
https://doi.org/10.1258/rsmsmj.52.1.28

Đurić M, Pavlica D, Janković L, Milašin J, Jovanović T. Presence of herpes simplex virus on the oral mucosa in patients undergoing chemotherapy. in Scottish Medical Journal. 2007;52(1):28-31.
doi:10.1258/rsmsmj.52.1.28 .

Đurić, M., Pavlica, Dušan, Janković, L., Milašin, Jelena, Jovanović, T., "Presence of herpes simplex virus on the oral mucosa in patients undergoing chemotherapy" in Scottish Medical Journal, 52, no. 1 (2007):28-31,
https://doi.org/10.1258/rsmsmj.52.1.28 . .

TY  - JOUR
AU  - Strahinić, I.
AU  - Busarcević, M.
AU  - Pavlica, Dušan
AU  - Milašin, Jelena
AU  - Golić, N.
AU  - Topisirović, L.
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1338
AB  - Introduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains.
PB  - Wiley, Hoboken
T2  - Oral Microbiology & Immunology
T1  - Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates
VL  - 22
IS  - 2
SP  - 111
EP  - 117
DO  - 10.1111/j.1399-302X.2007.00331.x
ER  - 

@article{
author = "Strahinić, I. and Busarcević, M. and Pavlica, Dušan and Milašin, Jelena and Golić, N. and Topisirović, L.",
year = "2007",
abstract = "Introduction: The objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. Methods: Samples were collected from four different locations within the oral cavity: surface of healthy tooth, oral mucous membrane, surface of tooth decay and deep tooth decay. On the basis of morphological and biochemical properties eight categories were formed and 26 isolates were selected for further characterization. The isolates were determined as Lactobacillus sp. using primers specific for 16S rDNA. Sequencing of 16S rDNA genes and repetitive sequence-based polymerase chain reactions were used for determination to species and subspecies levels. Results: Predominant species were Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus salivarius and Lactobacillus paracasei subsp. paracasei, while Lactobacillus acidophilus, Lactobacillus cellobiosus, Lactobacillus delbrueckii subsp. lactis and Lactobacillus gasseri were also present. The isolates Lactobacillus salivarius BGHO1, Lactobacillus fermentum BGHO36 and BGHO64, Lactobacillus gasseri BGHO89 and Lactobacillus delbrueckii subsp. lactis BGHO99 exhibited antagonistic action on the growth of Staphylococcus aureus, Enterococcus faecalis, Micrococcus flavus, Salmonella enteritidis, Streptococcus pneumoniae and Streptococcus mutans, but not on growth of Candida albicans. Moreover, the isolates L. salivarius BGHO1 and L. gasseri BGHO89 were tolerant to low pH and high concentration of bile salts. Conclusion: Taken together, these findings imply that L. salivarius BGHO1 and L. gasseri BGHO89 might be subjects for additional investigation as potential probiotic strains.",
publisher = "Wiley, Hoboken",
journal = "Oral Microbiology & Immunology",
title = "Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates",
volume = "22",
number = "2",
pages = "111-117",
doi = "10.1111/j.1399-302X.2007.00331.x"
}

Strahinić, I., Busarcević, M., Pavlica, D., Milašin, J., Golić, N.,& Topisirović, L.. (2007). Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. in Oral Microbiology & Immunology
Wiley, Hoboken., 22(2), 111-117.
https://doi.org/10.1111/j.1399-302X.2007.00331.x

Strahinić I, Busarcević M, Pavlica D, Milašin J, Golić N, Topisirović L. Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. in Oral Microbiology & Immunology. 2007;22(2):111-117.
doi:10.1111/j.1399-302X.2007.00331.x .

Strahinić, I., Busarcević, M., Pavlica, Dušan, Milašin, Jelena, Golić, N., Topisirović, L., "Molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates" in Oral Microbiology & Immunology, 22, no. 2 (2007):111-117,
https://doi.org/10.1111/j.1399-302X.2007.00331.x . .

TY  - JOUR
AU  - Todorović, Tatjana
AU  - Dožić, Ivan
AU  - Pavlica, Dušan
AU  - Marković, Dejan
AU  - Ivanović, Mirjana
AU  - Brajović, Gavrilo
AU  - Stefanović, Gordana
AU  - Mirković, Silvija
AU  - Anđelski, Biljana
PY  - 2005
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1233
AB  - Saliva is a secretion of the salivary and mucous glands and is of major importance in the maintainance of oral health. Over the last few decades, saliva has been evaluated as a diagnostic fluid in medicine for determining systemic disease markers as well as for monitoring numerous drugs, narcotics, and hormones. The biochemical analysis of saliva is particularly important in dentistry. The estimation of the risk of appearance and diagnosis of disease, monitoring of disease progression, evaluation of therapy efficacy for caries, periodontitis, premalignant and malignant oral lesions, as well as infectious diseases of the oral cavity, can be assessed by analyzing different constituent: of saliva, individuals at risk of caries can be identified using test: that determine saliva flow rate, saliva buffer capacity, and colonization of the oral cavity by cariogenic bacteria. Today, these rapid and simple diagnostic tests are used routinely in caries risk determination. The study and use of saliva-based diagnostics have increased over the last few decades. Clinical testing of saliva shows much promise. However, there is a need for much additional research in this area, before the true clinical value of saliva as a diagnostic fluid in dentistry can be determined.
AB  - Pljuvačka je sekret pljuvačnih i mukoznih žlezda koji je značajan za održavanje oralnog zdravlja. Poslednjih decenija sve više se razmatra mogućnost njene primene kao zamena krvi, odnosno krvnom serumu i plazme, radi dokazivanja pokazatelja sistemskih oboljenja, kao i za nadgledanje primene lekova, droga i hormona. Analiziranje biohemijskog sastava pljuvačke posebno je značajno u stomatologiji za procenu rizika nastanka, praćenja toka bolesti i kontrole rezultata primenjenog lečenja zubnog karijesa, parodontopatije, oralnih karcinoma i infektivnih oboljenja usne duplje. U proceni rizika nastanka karijesa na zubima rutinski se izvode ispitivanja kojima se određuju količina izlučene pljuvačke, njen puferski kapacitet i stepen kolonizacije usne duplje kariogenim vrstama bakterija. Jednostavnost izvođenja ovih ispitivanja omogućava da ih u stomatološkoj ordinaciji primenjuje lekar praktičar, što predstavlja veliku pomoć u proceni rizika za nastanak zubnog karijesa. Zbog bliskog kontakta s oralnim tkivima, pljuvačka sadrži brojne biohemijske pokazatelje patoloških procesa lokalizovanih u ovim tkivima (enzimi, imunoglobulini, ostali proteini, fenotipski pokazatelji). Ispitivanje pomenutih pokazatelja je značajno kao dopuna kliničkom dijagnostikovanju ukoliko ono ne pruža dovoljno informacija.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Use of saliva as a diagnostic fluid in dentistry
T1  - Mogućnosti upotrebe pljuvačke kao dijagnostičke tečnosti u stomatologiji
VL  - 133
IS  - 7-8
SP  - 372
EP  - 378
DO  - 10.2298/SARH0508372T
ER  - 

@article{
author = "Todorović, Tatjana and Dožić, Ivan and Pavlica, Dušan and Marković, Dejan and Ivanović, Mirjana and Brajović, Gavrilo and Stefanović, Gordana and Mirković, Silvija and Anđelski, Biljana",
year = "2005",
abstract = "Saliva is a secretion of the salivary and mucous glands and is of major importance in the maintainance of oral health. Over the last few decades, saliva has been evaluated as a diagnostic fluid in medicine for determining systemic disease markers as well as for monitoring numerous drugs, narcotics, and hormones. The biochemical analysis of saliva is particularly important in dentistry. The estimation of the risk of appearance and diagnosis of disease, monitoring of disease progression, evaluation of therapy efficacy for caries, periodontitis, premalignant and malignant oral lesions, as well as infectious diseases of the oral cavity, can be assessed by analyzing different constituent: of saliva, individuals at risk of caries can be identified using test: that determine saliva flow rate, saliva buffer capacity, and colonization of the oral cavity by cariogenic bacteria. Today, these rapid and simple diagnostic tests are used routinely in caries risk determination. The study and use of saliva-based diagnostics have increased over the last few decades. Clinical testing of saliva shows much promise. However, there is a need for much additional research in this area, before the true clinical value of saliva as a diagnostic fluid in dentistry can be determined., Pljuvačka je sekret pljuvačnih i mukoznih žlezda koji je značajan za održavanje oralnog zdravlja. Poslednjih decenija sve više se razmatra mogućnost njene primene kao zamena krvi, odnosno krvnom serumu i plazme, radi dokazivanja pokazatelja sistemskih oboljenja, kao i za nadgledanje primene lekova, droga i hormona. Analiziranje biohemijskog sastava pljuvačke posebno je značajno u stomatologiji za procenu rizika nastanka, praćenja toka bolesti i kontrole rezultata primenjenog lečenja zubnog karijesa, parodontopatije, oralnih karcinoma i infektivnih oboljenja usne duplje. U proceni rizika nastanka karijesa na zubima rutinski se izvode ispitivanja kojima se određuju količina izlučene pljuvačke, njen puferski kapacitet i stepen kolonizacije usne duplje kariogenim vrstama bakterija. Jednostavnost izvođenja ovih ispitivanja omogućava da ih u stomatološkoj ordinaciji primenjuje lekar praktičar, što predstavlja veliku pomoć u proceni rizika za nastanak zubnog karijesa. Zbog bliskog kontakta s oralnim tkivima, pljuvačka sadrži brojne biohemijske pokazatelje patoloških procesa lokalizovanih u ovim tkivima (enzimi, imunoglobulini, ostali proteini, fenotipski pokazatelji). Ispitivanje pomenutih pokazatelja je značajno kao dopuna kliničkom dijagnostikovanju ukoliko ono ne pruža dovoljno informacija.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Use of saliva as a diagnostic fluid in dentistry, Mogućnosti upotrebe pljuvačke kao dijagnostičke tečnosti u stomatologiji",
volume = "133",
number = "7-8",
pages = "372-378",
doi = "10.2298/SARH0508372T"
}

Todorović, T., Dožić, I., Pavlica, D., Marković, D., Ivanović, M., Brajović, G., Stefanović, G., Mirković, S.,& Anđelski, B.. (2005). Use of saliva as a diagnostic fluid in dentistry. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 133(7-8), 372-378.
https://doi.org/10.2298/SARH0508372T

Todorović T, Dožić I, Pavlica D, Marković D, Ivanović M, Brajović G, Stefanović G, Mirković S, Anđelski B. Use of saliva as a diagnostic fluid in dentistry. in Srpski arhiv za celokupno lekarstvo. 2005;133(7-8):372-378.
doi:10.2298/SARH0508372T .

Todorović, Tatjana, Dožić, Ivan, Pavlica, Dušan, Marković, Dejan, Ivanović, Mirjana, Brajović, Gavrilo, Stefanović, Gordana, Mirković, Silvija, Anđelski, Biljana, "Use of saliva as a diagnostic fluid in dentistry" in Srpski arhiv za celokupno lekarstvo, 133, no. 7-8 (2005):372-378,
https://doi.org/10.2298/SARH0508372T . .

TY  - JOUR
AU  - Jovanović, Tanja
AU  - Pavlica, Dušan
AU  - Knežević, Aleksandra
AU  - Lazarević, Ivana B.
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1211
AB  - It is well known that the most of AIDS patient have evidence of more severe herpetic infection than immunocompetent persons. In this study we examined the characteristics of humoral immune response during active herpes simplex virus infection in immunocompetent and immunocompromised patients, and correlate the value of specific markers (titre of specific anti-HSV-IgM, anti-HSV-IgG antibody, titre of neutralization antibody and avidity of IgG) between these 2 groups. The results have shown that although AIDS patients have severe depression of their cellular immune response, they can mount an intensive humoral antiviral response during the active HSV-1 infection. They have higher titre and avidity of specific anti-HSV antibody than immunocompetent persons. But even with high titre of specific antibody, AIDS patient have no enough neutralizing antibodies. According to the clinical manifestation of herpetic infection in AIDS patient, our result showed significant association between the quantity of in vitro neutralizing activity and in vivo protection from severe herpetic infection.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - Virus specific humoral immune response to Herpes Simplex Virus (HSV) in AIDS patients with oropharyngeal herpes simplex infection
VL  - 8
IS  - 2
SP  - 96
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_smile_1211
ER  - 

@article{
author = "Jovanović, Tanja and Pavlica, Dušan and Knežević, Aleksandra and Lazarević, Ivana B.",
year = "2004",
abstract = "It is well known that the most of AIDS patient have evidence of more severe herpetic infection than immunocompetent persons. In this study we examined the characteristics of humoral immune response during active herpes simplex virus infection in immunocompetent and immunocompromised patients, and correlate the value of specific markers (titre of specific anti-HSV-IgM, anti-HSV-IgG antibody, titre of neutralization antibody and avidity of IgG) between these 2 groups. The results have shown that although AIDS patients have severe depression of their cellular immune response, they can mount an intensive humoral antiviral response during the active HSV-1 infection. They have higher titre and avidity of specific anti-HSV antibody than immunocompetent persons. But even with high titre of specific antibody, AIDS patient have no enough neutralizing antibodies. According to the clinical manifestation of herpetic infection in AIDS patient, our result showed significant association between the quantity of in vitro neutralizing activity and in vivo protection from severe herpetic infection.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "Virus specific humoral immune response to Herpes Simplex Virus (HSV) in AIDS patients with oropharyngeal herpes simplex infection",
volume = "8",
number = "2",
pages = "96-98",
url = "https://hdl.handle.net/21.15107/rcub_smile_1211"
}

Jovanović, T., Pavlica, D., Knežević, A.,& Lazarević, I. B.. (2004). Virus specific humoral immune response to Herpes Simplex Virus (HSV) in AIDS patients with oropharyngeal herpes simplex infection. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 8(2), 96-98.
https://hdl.handle.net/21.15107/rcub_smile_1211

Jovanović T, Pavlica D, Knežević A, Lazarević IB. Virus specific humoral immune response to Herpes Simplex Virus (HSV) in AIDS patients with oropharyngeal herpes simplex infection. in Balkan Journal of Stomatology. 2004;8(2):96-98.
https://hdl.handle.net/21.15107/rcub_smile_1211 .

Jovanović, Tanja, Pavlica, Dušan, Knežević, Aleksandra, Lazarević, Ivana B., "Virus specific humoral immune response to Herpes Simplex Virus (HSV) in AIDS patients with oropharyngeal herpes simplex infection" in Balkan Journal of Stomatology, 8, no. 2 (2004):96-98,
https://hdl.handle.net/21.15107/rcub_smile_1211 .

TY  - JOUR
AU  - Marković, Dejan
AU  - Pavlica, Dušan
AU  - Perić, Tamara
PY  - 2003
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1180
AB  - The aim of the study was to assess the efficacy of the chemo-mechani- cal method for caries removal based on microbiological analyses of dentine samples. Sixty freshly extracted teeth with coronaly caries were analysed. In the experimental group (30 teeth) chemo-mechanical caries removal was performed, and in the control group (30 teeth) rotary instruments were used. Before the caries treatment and at the completion of cavity preparation dentine samples were taken by sterile round ISO 012 bur for microbiological analyses. Results of the study showed significant differences between initial and final dentine samples, while differences between the experimental and control group were not significant. The microbiological analyses in the present study imply that the chemo-mechanical method for caries removal is as efficient as the conventional technique.
AB  - Cilj rada je bio da se mikrobiološkom analizom uzoraka dentina sa dna preparisanog kaviteta proceni kvalitet i efikasnost hemijsko-mehaničke metode u uklanjanju karijesa. Istraživanje je obavljeno na 60 sveže ekstrahovanih zuba na kojima je dijagnostikovan koronarni karijes. Nakon ekstrakcije, zubi su podeljeni u dve grupe: u eksperimentalnoj grupi (30 zuba) primenjena je hemijsko-mehanička metoda uklanjanja karijesa, dok je u kontrolnoj grupi (30 zuba) karijes uklanjan mašinskim rotirajućim instrumentima. Neposredno pre i nakon terapijske procedure, sterilnim okruglim ISO 012 borerom uzimani su uzorci dentinskog detritusa za mikrobiološku analizu. Dobijeni rezultati ukazuju na značajnu razliku u broju bakterija između početnih i finalnih uzoraka dentina, ali bez značajnih razlika u eksperimentalnoj i kontrolnoj grupi. Rezultati istraživanja ukazuju na efikasnost hemijsko-mehaničke metode u uklanjanju karijesom oštećenih zubnih tkiva.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Microbiological assessment of the chemo-mechanical caries removal
T1  - Mikrobiološka analiza efikasnosti hemijsko-mehaničke metode uklanjanja karijesa dentina
VL  - 50
IS  - 4
SP  - 192
EP  - 196
DO  - 10.2298/SGS0304192M
ER  - 

@article{
author = "Marković, Dejan and Pavlica, Dušan and Perić, Tamara",
year = "2003",
abstract = "The aim of the study was to assess the efficacy of the chemo-mechani- cal method for caries removal based on microbiological analyses of dentine samples. Sixty freshly extracted teeth with coronaly caries were analysed. In the experimental group (30 teeth) chemo-mechanical caries removal was performed, and in the control group (30 teeth) rotary instruments were used. Before the caries treatment and at the completion of cavity preparation dentine samples were taken by sterile round ISO 012 bur for microbiological analyses. Results of the study showed significant differences between initial and final dentine samples, while differences between the experimental and control group were not significant. The microbiological analyses in the present study imply that the chemo-mechanical method for caries removal is as efficient as the conventional technique., Cilj rada je bio da se mikrobiološkom analizom uzoraka dentina sa dna preparisanog kaviteta proceni kvalitet i efikasnost hemijsko-mehaničke metode u uklanjanju karijesa. Istraživanje je obavljeno na 60 sveže ekstrahovanih zuba na kojima je dijagnostikovan koronarni karijes. Nakon ekstrakcije, zubi su podeljeni u dve grupe: u eksperimentalnoj grupi (30 zuba) primenjena je hemijsko-mehanička metoda uklanjanja karijesa, dok je u kontrolnoj grupi (30 zuba) karijes uklanjan mašinskim rotirajućim instrumentima. Neposredno pre i nakon terapijske procedure, sterilnim okruglim ISO 012 borerom uzimani su uzorci dentinskog detritusa za mikrobiološku analizu. Dobijeni rezultati ukazuju na značajnu razliku u broju bakterija između početnih i finalnih uzoraka dentina, ali bez značajnih razlika u eksperimentalnoj i kontrolnoj grupi. Rezultati istraživanja ukazuju na efikasnost hemijsko-mehaničke metode u uklanjanju karijesom oštećenih zubnih tkiva.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Microbiological assessment of the chemo-mechanical caries removal, Mikrobiološka analiza efikasnosti hemijsko-mehaničke metode uklanjanja karijesa dentina",
volume = "50",
number = "4",
pages = "192-196",
doi = "10.2298/SGS0304192M"
}

Marković, D., Pavlica, D.,& Perić, T.. (2003). Microbiological assessment of the chemo-mechanical caries removal. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 50(4), 192-196.
https://doi.org/10.2298/SGS0304192M

Marković D, Pavlica D, Perić T. Microbiological assessment of the chemo-mechanical caries removal. in Stomatološki glasnik Srbije. 2003;50(4):192-196.
doi:10.2298/SGS0304192M .

Marković, Dejan, Pavlica, Dušan, Perić, Tamara, "Microbiological assessment of the chemo-mechanical caries removal" in Stomatološki glasnik Srbije, 50, no. 4 (2003):192-196,
https://doi.org/10.2298/SGS0304192M . .

TY  - JOUR
AU  - Pavlica, Dušan
PY  - 2002
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1145
AB  - In this study we investigated the possibility of saliva use instead of sera for detecting anti HSV IgM antibodies. Three groups of patients were included in this study. The first two consisted of immunocompetent persons with clinically and virologicaly approved primary and reccurent herpetic infection. The third group was comprised of AIDS patients with recurrent infection. Anti HSV-1 IgM antibodies were detected by immunofluorescence assay. In saliva and sera samples obtained from persons with primary and recurrent HSV infection, findings of specific IgM antibodies correlated with the dynamic of their synthesis in infection generally. But in the third group, which consisted of AIDS patients, in a few cases there were positive anti HSV IgM concentration, both in sera and saliva samples.
AB  - U radu je ispitivana mogućnost upotrebe salive kao materijala alternativnog serumu za dokazivanje anti HSV-1 IgM antitela. U studiju su uključene tri grupe pacijenata od kojih su prve dve činili imunokompetentne osobe sa klinički i virusološki potvrđenom HSV infekcijom, a u treću grupu su bili uključeni pacijenti oboleli od AIDS-a koji su pored mnogobrojnih oportunstičkih infekcija imali i herpetičnu infekciju u formi labijalnog herpesa. Prisustvo anti HSV-1 IgM antitela dokazivano je pomoću testa indirektne imuno fluorescencije u uzorcima seruma i salive svih pacijenata. Nalaz anti IISV-I IgM antitela u obe vrste uzoraka u imunikompetentnih osoba sa primarnom i sekundarnom herpetičnom infekcijom bio je u skladu sa dinamikom njihove sinteze tokom infekcije uopšte. Međutim u grupi pacijenata sa AIDS-om, bez obzira što je kod njih bila prisutna rekurentna herpetična infekcija, u određenom broju slučajeva dokazan je pozitivan nalaz anti HSV-1 IgM antitela u serumu odnosno, salivi.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Possibility of saliva use in detecting anti herpes simplex virus IgM antibodies
T1  - Mogućnost primene salive za dokazivanje anti herpes simplex virusa IgM antitela
VL  - 49
IS  - 1-2
SP  - 46
EP  - 49
DO  - 10.2298/SGS0202046P
ER  - 

@article{
author = "Pavlica, Dušan",
year = "2002",
abstract = "In this study we investigated the possibility of saliva use instead of sera for detecting anti HSV IgM antibodies. Three groups of patients were included in this study. The first two consisted of immunocompetent persons with clinically and virologicaly approved primary and reccurent herpetic infection. The third group was comprised of AIDS patients with recurrent infection. Anti HSV-1 IgM antibodies were detected by immunofluorescence assay. In saliva and sera samples obtained from persons with primary and recurrent HSV infection, findings of specific IgM antibodies correlated with the dynamic of their synthesis in infection generally. But in the third group, which consisted of AIDS patients, in a few cases there were positive anti HSV IgM concentration, both in sera and saliva samples., U radu je ispitivana mogućnost upotrebe salive kao materijala alternativnog serumu za dokazivanje anti HSV-1 IgM antitela. U studiju su uključene tri grupe pacijenata od kojih su prve dve činili imunokompetentne osobe sa klinički i virusološki potvrđenom HSV infekcijom, a u treću grupu su bili uključeni pacijenti oboleli od AIDS-a koji su pored mnogobrojnih oportunstičkih infekcija imali i herpetičnu infekciju u formi labijalnog herpesa. Prisustvo anti HSV-1 IgM antitela dokazivano je pomoću testa indirektne imuno fluorescencije u uzorcima seruma i salive svih pacijenata. Nalaz anti IISV-I IgM antitela u obe vrste uzoraka u imunikompetentnih osoba sa primarnom i sekundarnom herpetičnom infekcijom bio je u skladu sa dinamikom njihove sinteze tokom infekcije uopšte. Međutim u grupi pacijenata sa AIDS-om, bez obzira što je kod njih bila prisutna rekurentna herpetična infekcija, u određenom broju slučajeva dokazan je pozitivan nalaz anti HSV-1 IgM antitela u serumu odnosno, salivi.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Possibility of saliva use in detecting anti herpes simplex virus IgM antibodies, Mogućnost primene salive za dokazivanje anti herpes simplex virusa IgM antitela",
volume = "49",
number = "1-2",
pages = "46-49",
doi = "10.2298/SGS0202046P"
}

Pavlica, D.. (2002). Possibility of saliva use in detecting anti herpes simplex virus IgM antibodies. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 49(1-2), 46-49.
https://doi.org/10.2298/SGS0202046P

Pavlica D. Possibility of saliva use in detecting anti herpes simplex virus IgM antibodies. in Stomatološki glasnik Srbije. 2002;49(1-2):46-49.
doi:10.2298/SGS0202046P .

Pavlica, Dušan, "Possibility of saliva use in detecting anti herpes simplex virus IgM antibodies" in Stomatološki glasnik Srbije, 49, no. 1-2 (2002):46-49,
https://doi.org/10.2298/SGS0202046P . .

TY  - JOUR
AU  - Živković, Slavoljub
AU  - Bojović, S.
AU  - Pavlica, Dušan
PY  - 2001
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1129
AB  - The aim of this study was to assess the quality of the marginal seals of 7 restoratives by means of a bacterial penetration test in vitro. Sixty intact premolars and third molars that were scheduled for extraction were used in the test. There were 2 experimental groups of teeth, as follows: (1)A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface, respectively, of each tooth. (2) A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface. respectively, of each tooth with a completely removed enamel layer. The cavities were then reconstructed with different restorative materials. The quality of the marginal seals was evaluated by submerging the teeth in a bacterial suspension and incubating them in an anaerobic milieu at 37 degreesC for 20 hours. The teeth were subsequently processed for histologic data and bacterial staining. The best marginal sealing in both the wedge erosion and the class V cavities was provided by the Herculite/Optibond system and the Valux Plus/Scotchbond Multipurpose system. Bacterial penetration was slightly greater with the Luxat compomer and the Dyrect compomer, as well as with Vitremer glass ionomer cement and Fuji LC glass ionomer cement. The bacterial penetration test showed that the use of restorative material does not entirely eliminate microleakage.
PB  - Mosby-Elsevier, New York
T2  - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology
T1  - Bacterial penetration of restored cavities
VL  - 91
IS  - 3
SP  - 353
EP  - 358
DO  - 10.1067/moe.2001.113345
ER  - 

@article{
author = "Živković, Slavoljub and Bojović, S. and Pavlica, Dušan",
year = "2001",
abstract = "The aim of this study was to assess the quality of the marginal seals of 7 restoratives by means of a bacterial penetration test in vitro. Sixty intact premolars and third molars that were scheduled for extraction were used in the test. There were 2 experimental groups of teeth, as follows: (1)A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface, respectively, of each tooth. (2) A class V conventional cavity and a wedge erosion cavity were prepared on the buccal surface and the lingual surface. respectively, of each tooth with a completely removed enamel layer. The cavities were then reconstructed with different restorative materials. The quality of the marginal seals was evaluated by submerging the teeth in a bacterial suspension and incubating them in an anaerobic milieu at 37 degreesC for 20 hours. The teeth were subsequently processed for histologic data and bacterial staining. The best marginal sealing in both the wedge erosion and the class V cavities was provided by the Herculite/Optibond system and the Valux Plus/Scotchbond Multipurpose system. Bacterial penetration was slightly greater with the Luxat compomer and the Dyrect compomer, as well as with Vitremer glass ionomer cement and Fuji LC glass ionomer cement. The bacterial penetration test showed that the use of restorative material does not entirely eliminate microleakage.",
publisher = "Mosby-Elsevier, New York",
journal = "Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology",
title = "Bacterial penetration of restored cavities",
volume = "91",
number = "3",
pages = "353-358",
doi = "10.1067/moe.2001.113345"
}

Živković, S., Bojović, S.,& Pavlica, D.. (2001). Bacterial penetration of restored cavities. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology
Mosby-Elsevier, New York., 91(3), 353-358.
https://doi.org/10.1067/moe.2001.113345

Živković S, Bojović S, Pavlica D. Bacterial penetration of restored cavities. in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology. 2001;91(3):353-358.
doi:10.1067/moe.2001.113345 .

Živković, Slavoljub, Bojović, S., Pavlica, Dušan, "Bacterial penetration of restored cavities" in Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontology, 91, no. 3 (2001):353-358,
https://doi.org/10.1067/moe.2001.113345 . .

TY  - THES
AU  - Pavlica, Dušan
PY  - 1991
UR  - https://plus.sr.cobiss.net/opac7/bib/1024083598
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/265
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Nalaz i distribucija gljivica iz roda Candida u usnoj duplji u osoba sa imunodeficijencijom
UR  - https://hdl.handle.net/21.15107/rcub_smile_265
ER  - 

@mastersthesis{
author = "Pavlica, Dušan",
year = "1991",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Nalaz i distribucija gljivica iz roda Candida u usnoj duplji u osoba sa imunodeficijencijom",
url = "https://hdl.handle.net/21.15107/rcub_smile_265"
}

Pavlica, D.. (1991). Nalaz i distribucija gljivica iz roda Candida u usnoj duplji u osoba sa imunodeficijencijom. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_smile_265

Pavlica D. Nalaz i distribucija gljivica iz roda Candida u usnoj duplji u osoba sa imunodeficijencijom. 1991;.
https://hdl.handle.net/21.15107/rcub_smile_265 .

Pavlica, Dušan, "Nalaz i distribucija gljivica iz roda Candida u usnoj duplji u osoba sa imunodeficijencijom" (1991),
https://hdl.handle.net/21.15107/rcub_smile_265 .