TY  - JOUR
AU  - Grubiša, Ivana
AU  - Otašević, Petar
AU  - Vucinić, Nada
AU  - Miličić, Biljana
AU  - Jozić, Tanja
AU  - Krstić, Slobodan
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2287
AB  - Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold increase in atherosclerosis risk (OR: 2.1, 95%CI=1.39-3.17, P=0.0004). GSTT1 null genotype alone did not show a statistically significant effect on atherosclerosis risk modulation, but the association approached significance (OR: 1.57, 95%CI=0.94-2.64, P=0.08). The combined analysis showed that the presence of both genes had a protective effect against atherosclerosis (OR=0.55, 95%CI=0.37-0.83, P=0.005) while double null genotypes led to a robust atherosclerosis risk increase (OR: 8.14, 95%CI=2.41-27.51, P  lt  0.0001). This study demonstrated that the GSTM1 null and combined GSTM1/GSTT1 null genotypes are susceptibility factors for development of atherosclerosis in a Serbian population.
PB  - Soc Brasil Genetica, Ribeirao Pret
T2  - Genetics & Molecular Biology
T1  - Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population
VL  - 41
IS  - 1
SP  - 35
EP  - 40
DO  - 10.1590/1678-4685-GMB-2017-0034
ER  - 

@article{
author = "Grubiša, Ivana and Otašević, Petar and Vucinić, Nada and Miličić, Biljana and Jozić, Tanja and Krstić, Slobodan and Milašin, Jelena",
year = "2018",
abstract = "Oxidative stress (OS) plays an important role in atherogenesis and since glutathione S-transferases (GSTs) provide protection against OS, we have tested the hypothesis that deletion polymorphisms in two GSTs (GSTM1 and GSTT1) may affect the risk of developing atherosclerosis. A total of 382 individuals (200 patients with atherosclerosis and 182 healthy controls) were included in this association study. Genomic DNA was isolated from peripheral blood cells or from buccal epithelial cells and genotyping was performed using multiplex-PCR or real-time PCR methods. GSTM1 null genotype was significantly more frequent in atherosclerotic patients than in controls (52.0% vs 34.1%) and individuals with the GSTM1 null genotype had an approximately 2-fold increase in atherosclerosis risk (OR: 2.1, 95%CI=1.39-3.17, P=0.0004). GSTT1 null genotype alone did not show a statistically significant effect on atherosclerosis risk modulation, but the association approached significance (OR: 1.57, 95%CI=0.94-2.64, P=0.08). The combined analysis showed that the presence of both genes had a protective effect against atherosclerosis (OR=0.55, 95%CI=0.37-0.83, P=0.005) while double null genotypes led to a robust atherosclerosis risk increase (OR: 8.14, 95%CI=2.41-27.51, P  lt  0.0001). This study demonstrated that the GSTM1 null and combined GSTM1/GSTT1 null genotypes are susceptibility factors for development of atherosclerosis in a Serbian population.",
publisher = "Soc Brasil Genetica, Ribeirao Pret",
journal = "Genetics & Molecular Biology",
title = "Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population",
volume = "41",
number = "1",
pages = "35-40",
doi = "10.1590/1678-4685-GMB-2017-0034"
}

Grubiša, I., Otašević, P., Vucinić, N., Miličić, B., Jozić, T., Krstić, S.,& Milašin, J.. (2018). Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population. in Genetics & Molecular Biology
Soc Brasil Genetica, Ribeirao Pret., 41(1), 35-40.
https://doi.org/10.1590/1678-4685-GMB-2017-0034

Grubiša I, Otašević P, Vucinić N, Miličić B, Jozić T, Krstić S, Milašin J. Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population. in Genetics & Molecular Biology. 2018;41(1):35-40.
doi:10.1590/1678-4685-GMB-2017-0034 .

Grubiša, Ivana, Otašević, Petar, Vucinić, Nada, Miličić, Biljana, Jozić, Tanja, Krstić, Slobodan, Milašin, Jelena, "Combined GSTM1 and GSTT1 null genotypes are strong risk factors for atherogenesis in a Serbian population" in Genetics & Molecular Biology, 41, no. 1 (2018):35-40,
https://doi.org/10.1590/1678-4685-GMB-2017-0034 . .

TY  - JOUR
AU  - Ilić, Branislav
AU  - Nikolić, Nadja
AU  - Andrić, Miroslav
AU  - Jelovac, Drago
AU  - Miličić, Biljana
AU  - Jozić, Tanja
AU  - Krstić, Slobodan
AU  - Milašin, Jelena
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2190
AB  - BACKGROUND: Polymorphisms in genes encoding tumor necrosis factor-alpha (TNF-alpha) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-alpha (-308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients. METHODS: Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis. RESULTS: A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P  lt  0.05). Carriers of the TNF-alpha A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98-16.56, P  lt  0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR= 3.65, CI: 1.60-8.40, P = 0.001). CONCLUSIONS: Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population.
PB  - Wiley, Hoboken
T2  - Journal of Oral Pathology & Medicine
T1  - TNF-alpha (-308G > A) and TNF-R1 (36A > G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development
VL  - 46
IS  - 4
SP  - 292
EP  - 296
DO  - 10.1111/jop.12564
ER  - 

@article{
author = "Ilić, Branislav and Nikolić, Nadja and Andrić, Miroslav and Jelovac, Drago and Miličić, Biljana and Jozić, Tanja and Krstić, Slobodan and Milašin, Jelena",
year = "2017",
abstract = "BACKGROUND: Polymorphisms in genes encoding tumor necrosis factor-alpha (TNF-alpha) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-alpha (-308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients. METHODS: Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis. RESULTS: A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P  lt  0.05). Carriers of the TNF-alpha A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98-16.56, P  lt  0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR= 3.65, CI: 1.60-8.40, P = 0.001). CONCLUSIONS: Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population.",
publisher = "Wiley, Hoboken",
journal = "Journal of Oral Pathology & Medicine",
title = "TNF-alpha (-308G > A) and TNF-R1 (36A > G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development",
volume = "46",
number = "4",
pages = "292-296",
doi = "10.1111/jop.12564"
}

Ilić, B., Nikolić, N., Andrić, M., Jelovac, D., Miličić, B., Jozić, T., Krstić, S.,& Milašin, J.. (2017). TNF-alpha (-308G > A) and TNF-R1 (36A > G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development. in Journal of Oral Pathology & Medicine
Wiley, Hoboken., 46(4), 292-296.
https://doi.org/10.1111/jop.12564

Ilić B, Nikolić N, Andrić M, Jelovac D, Miličić B, Jozić T, Krstić S, Milašin J. TNF-alpha (-308G > A) and TNF-R1 (36A > G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development. in Journal of Oral Pathology & Medicine. 2017;46(4):292-296.
doi:10.1111/jop.12564 .

Ilić, Branislav, Nikolić, Nadja, Andrić, Miroslav, Jelovac, Drago, Miličić, Biljana, Jozić, Tanja, Krstić, Slobodan, Milašin, Jelena, "TNF-alpha (-308G > A) and TNF-R1 (36A > G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development" in Journal of Oral Pathology & Medicine, 46, no. 4 (2017):292-296,
https://doi.org/10.1111/jop.12564 . .

TY  - JOUR
AU  - Jeremić, Vasilije
AU  - Alempijević, Tamara
AU  - Mijatović, Srđan
AU  - Sijacki, Ana
AU  - Dragašević, Sanja
AU  - Pavlović, Sonja
AU  - Miličić, Biljana
AU  - Krstić, Slobodan
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1884
AB  - In polytrauma, injuries that may be surgically treated under regular circumstances due to a systemic inflammatory response become life-threatening. The inflammatory response involves a complex pattern of humoral and cellular responses and the expression of related factors is thought to be governed by genetic variations. This aim of this paper is to examine the influence of interleukin (IL) 6 single nucleotide polymorphism (SNP) -174C/G and -596G/A on the treatment outcome in severely injured patients. Forty-seven severely injured patients were included in this study. Patients were assigned an Injury Severity Score. Blood samples were drawn within 24 h after admission (designated day 1) and on subsequent days (24, 48, 72, hours and 7days) of hospitalization. The IL-6 levels were determined through ELSA technique. Polymorphisms were analyzed by a method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR). Among subjects with different outcomes, no statistically relevant difference was found with regards to the gene IL-6 SNP-174G/C polymorphism. More than a half of subjects who died had the SNP-174G/C polymorphism, while this polymorphism was represented in a slightly lower number in survivors. The incidence of subjects without polymorphism and those with heterozygous and homozygous gene IL-6 SNP-596G/A polymorphism did not present statistically significant variations between survivors and those who died. The levels of IL-6 over the observation period did not present any statistically relevant difference among subjects without the IL-6 SNP-174 or IL- 6 SNP -596 gene polymorphism and those who had either a heterozygous or a homozygous polymorphism.
PB  - Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo, Cekalusa
T2  - Bosnian Journal of Basic Medical Sciences
T1  - Clinical relevance of IL-6 gene polymorphism in severely injured patients
VL  - 14
IS  - 2
SP  - 110
EP  - 117
DO  - 10.17305/bjbms.2014.2274
ER  - 

@article{
author = "Jeremić, Vasilije and Alempijević, Tamara and Mijatović, Srđan and Sijacki, Ana and Dragašević, Sanja and Pavlović, Sonja and Miličić, Biljana and Krstić, Slobodan",
year = "2014",
abstract = "In polytrauma, injuries that may be surgically treated under regular circumstances due to a systemic inflammatory response become life-threatening. The inflammatory response involves a complex pattern of humoral and cellular responses and the expression of related factors is thought to be governed by genetic variations. This aim of this paper is to examine the influence of interleukin (IL) 6 single nucleotide polymorphism (SNP) -174C/G and -596G/A on the treatment outcome in severely injured patients. Forty-seven severely injured patients were included in this study. Patients were assigned an Injury Severity Score. Blood samples were drawn within 24 h after admission (designated day 1) and on subsequent days (24, 48, 72, hours and 7days) of hospitalization. The IL-6 levels were determined through ELSA technique. Polymorphisms were analyzed by a method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR). Among subjects with different outcomes, no statistically relevant difference was found with regards to the gene IL-6 SNP-174G/C polymorphism. More than a half of subjects who died had the SNP-174G/C polymorphism, while this polymorphism was represented in a slightly lower number in survivors. The incidence of subjects without polymorphism and those with heterozygous and homozygous gene IL-6 SNP-596G/A polymorphism did not present statistically significant variations between survivors and those who died. The levels of IL-6 over the observation period did not present any statistically relevant difference among subjects without the IL-6 SNP-174 or IL- 6 SNP -596 gene polymorphism and those who had either a heterozygous or a homozygous polymorphism.",
publisher = "Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo, Cekalusa",
journal = "Bosnian Journal of Basic Medical Sciences",
title = "Clinical relevance of IL-6 gene polymorphism in severely injured patients",
volume = "14",
number = "2",
pages = "110-117",
doi = "10.17305/bjbms.2014.2274"
}

Jeremić, V., Alempijević, T., Mijatović, S., Sijacki, A., Dragašević, S., Pavlović, S., Miličić, B.,& Krstić, S.. (2014). Clinical relevance of IL-6 gene polymorphism in severely injured patients. in Bosnian Journal of Basic Medical Sciences
Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo, Cekalusa., 14(2), 110-117.
https://doi.org/10.17305/bjbms.2014.2274

Jeremić V, Alempijević T, Mijatović S, Sijacki A, Dragašević S, Pavlović S, Miličić B, Krstić S. Clinical relevance of IL-6 gene polymorphism in severely injured patients. in Bosnian Journal of Basic Medical Sciences. 2014;14(2):110-117.
doi:10.17305/bjbms.2014.2274 .

Jeremić, Vasilije, Alempijević, Tamara, Mijatović, Srđan, Sijacki, Ana, Dragašević, Sanja, Pavlović, Sonja, Miličić, Biljana, Krstić, Slobodan, "Clinical relevance of IL-6 gene polymorphism in severely injured patients" in Bosnian Journal of Basic Medical Sciences, 14, no. 2 (2014):110-117,
https://doi.org/10.17305/bjbms.2014.2274 . .