TY  - JOUR
AU  - Magić, Marko
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Vasilijić, Saša
AU  - Marković, Milan
AU  - Vučević, Dragana
AU  - Rudolf, Rebeka
AU  - Čolić, Snježana
AU  - Čolić, Miodrag
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2445
AB  - Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo.
AB  - Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition
T1  - Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme
VL  - 76
IS  - 5
SP  - 492
EP  - 501
DO  - 10.2298/VSP170809097M
ER  - 

@article{
author = "Magić, Marko and Čolović, Božana and Jokanović, Vukoman and Vasilijić, Saša and Marković, Milan and Vučević, Dragana and Rudolf, Rebeka and Čolić, Snježana and Čolić, Miodrag",
year = "2019",
abstract = "Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo., Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition, Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme",
volume = "76",
number = "5",
pages = "492-501",
doi = "10.2298/VSP170809097M"
}

Magić, M., Čolović, B., Jokanović, V., Vasilijić, S., Marković, M., Vučević, D., Rudolf, R., Čolić, S.,& Čolić, M.. (2019). Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(5), 492-501.
https://doi.org/10.2298/VSP170809097M

Magić M, Čolović B, Jokanović V, Vasilijić S, Marković M, Vučević D, Rudolf R, Čolić S, Čolić M. Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled. 2019;76(5):492-501.
doi:10.2298/VSP170809097M .

Magić, Marko, Čolović, Božana, Jokanović, Vukoman, Vasilijić, Saša, Marković, Milan, Vučević, Dragana, Rudolf, Rebeka, Čolić, Snježana, Čolić, Miodrag, "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition" in Vojnosanitetski pregled, 76, no. 5 (2019):492-501,
https://doi.org/10.2298/VSP170809097M . .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Tomić, Sergej
AU  - Rudolf, Rebeka
AU  - Marković, Evgenija
AU  - Šćepan, Ivana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2090
AB  - Superelastic (SE) and thermo-activated (TA) nickel-titanium (NiTi) archwires are used in everyday orthodontic practice, based on their acceptable biocompatibility and well-defined shape memory properties. However, the differences in their surface microstructure and cytotoxicity have not been clearly defined, and the standard cytotoxicity tests are too robust to detect small differences in the cytotoxicity of these alloys, all of which can lead to unexpected adverse reactions in some patients. Therefore, we tested the hypothesis that the differences in manufacture and microstructure of commercially available SE and TA archwires may influence their biocompatibility. The archwires were studied as-received and after conditioning for 24 h or 35 days in a cell culture medium under static conditions. All of the tested archwires, including their conditioned medium (CM), were non-cytotoxic for L929 cells, but Rematitan SE (both as received and conditioned) induced the apoptosis of rat thymocytes in a direct contact. In contrast, TruFlex SE and Equire TA increased the proliferation of thymocytes. The cytotoxic effect of Rematitan SE correlated with the higher release of Ni ions in CM, higher concentration of surface Ni and an increased oxygen layer thickness after the conditioning. In conclusion, the apoptosis assay on rat thymocytes, in contrast to the less sensitive standard assay on L929 cells, revealed that Rematitan SE was less cytocompatible compared to other archwires and the effect was most probably associated with a higher exposition of the cells to Ni on the surface of the archwire, due to the formation of unstable oxide layer.
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires
VL  - 27
IS  - 8
DO  - 10.1007/s10856-016-5742-1
ER  - 

@article{
author = "Čolić, Miodrag and Tomić, Sergej and Rudolf, Rebeka and Marković, Evgenija and Šćepan, Ivana",
year = "2016",
abstract = "Superelastic (SE) and thermo-activated (TA) nickel-titanium (NiTi) archwires are used in everyday orthodontic practice, based on their acceptable biocompatibility and well-defined shape memory properties. However, the differences in their surface microstructure and cytotoxicity have not been clearly defined, and the standard cytotoxicity tests are too robust to detect small differences in the cytotoxicity of these alloys, all of which can lead to unexpected adverse reactions in some patients. Therefore, we tested the hypothesis that the differences in manufacture and microstructure of commercially available SE and TA archwires may influence their biocompatibility. The archwires were studied as-received and after conditioning for 24 h or 35 days in a cell culture medium under static conditions. All of the tested archwires, including their conditioned medium (CM), were non-cytotoxic for L929 cells, but Rematitan SE (both as received and conditioned) induced the apoptosis of rat thymocytes in a direct contact. In contrast, TruFlex SE and Equire TA increased the proliferation of thymocytes. The cytotoxic effect of Rematitan SE correlated with the higher release of Ni ions in CM, higher concentration of surface Ni and an increased oxygen layer thickness after the conditioning. In conclusion, the apoptosis assay on rat thymocytes, in contrast to the less sensitive standard assay on L929 cells, revealed that Rematitan SE was less cytocompatible compared to other archwires and the effect was most probably associated with a higher exposition of the cells to Ni on the surface of the archwire, due to the formation of unstable oxide layer.",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires",
volume = "27",
number = "8",
doi = "10.1007/s10856-016-5742-1"
}

Čolić, M., Tomić, S., Rudolf, R., Marković, E.,& Šćepan, I.. (2016). Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 27(8).
https://doi.org/10.1007/s10856-016-5742-1

Čolić M, Tomić S, Rudolf R, Marković E, Šćepan I. Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires. in Journal of Materials Science-Materials in Medicine. 2016;27(8).
doi:10.1007/s10856-016-5742-1 .

Čolić, Miodrag, Tomić, Sergej, Rudolf, Rebeka, Marković, Evgenija, Šćepan, Ivana, "Differences in cytocompatibility, dynamics of the oxide layers' formation, and nickel release between superelastic and thermo-activated nickel-titanium archwires" in Journal of Materials Science-Materials in Medicine, 27, no. 8 (2016),
https://doi.org/10.1007/s10856-016-5742-1 . .

TY  - JOUR
AU  - Daković, Dragana
AU  - Čolić, Miodrag
AU  - Čakić, Saša
AU  - Mileusnić, Ivan
AU  - Hajduković, Zoran
AU  - Stamatović, Novak
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1852
AB  - Objective: The aim of this study was to investigate the differences between the salivary levels of IL-8 in patients with Type I diabetes mellitus (DM) with (DM+P) or without (DM-P) concomitant periodontitis and healthy subjects. The correlations between the levels of these cytokines and clinical periodontal parameters were also established Methods: Twenty children and adolescents with Type I DM (10 diagnosed with periodontitis, 10 presenting no signs of periodontitis) and a control group consisting of 20 healthy children and adolescents aged 7-18 years were recruited for this study. Results: The Salivary IL-8 level was statistically significantly (p lt 0.005) elevated in subjects with Type 1 DM (474.47 +/- 716.76) compared to non-diabetic control group (101.99 +/- 68.32). There was no difference (p>0.05) in the salivary IL-8 level when subjects with Type I DM with concomitant periodontitis were compared to diabetics without periodontitis. When the salivary IL-8 level in subjects with Type I DM was correlated with the clinical parameters, no statistical significance was found Conclusion: An elevated salivary IL-8 level in subjects with Type 1 DM without concomitant periodontitis plays a major role in the development of diabetic micro and macroangiopathy and pathogenesis of atherosclerosis. Consequently, this may offer a basis for the assessment of risk, prophylaxis and treatment of diabetic complications.
PB  - Journal Pedodontics Inc, Birmingham
T2  - Journal of Clinical Pediatric Dentistry
T1  - Salivary interleukin-8 levels in children suffering from Type 1 diabetes mellitus
VL  - 37
IS  - 4
SP  - 377
EP  - 380
DO  - 10.17796/jcpd.37.4.l135531h4542gj66
ER  - 

@article{
author = "Daković, Dragana and Čolić, Miodrag and Čakić, Saša and Mileusnić, Ivan and Hajduković, Zoran and Stamatović, Novak",
year = "2013",
abstract = "Objective: The aim of this study was to investigate the differences between the salivary levels of IL-8 in patients with Type I diabetes mellitus (DM) with (DM+P) or without (DM-P) concomitant periodontitis and healthy subjects. The correlations between the levels of these cytokines and clinical periodontal parameters were also established Methods: Twenty children and adolescents with Type I DM (10 diagnosed with periodontitis, 10 presenting no signs of periodontitis) and a control group consisting of 20 healthy children and adolescents aged 7-18 years were recruited for this study. Results: The Salivary IL-8 level was statistically significantly (p lt 0.005) elevated in subjects with Type 1 DM (474.47 +/- 716.76) compared to non-diabetic control group (101.99 +/- 68.32). There was no difference (p>0.05) in the salivary IL-8 level when subjects with Type I DM with concomitant periodontitis were compared to diabetics without periodontitis. When the salivary IL-8 level in subjects with Type I DM was correlated with the clinical parameters, no statistical significance was found Conclusion: An elevated salivary IL-8 level in subjects with Type 1 DM without concomitant periodontitis plays a major role in the development of diabetic micro and macroangiopathy and pathogenesis of atherosclerosis. Consequently, this may offer a basis for the assessment of risk, prophylaxis and treatment of diabetic complications.",
publisher = "Journal Pedodontics Inc, Birmingham",
journal = "Journal of Clinical Pediatric Dentistry",
title = "Salivary interleukin-8 levels in children suffering from Type 1 diabetes mellitus",
volume = "37",
number = "4",
pages = "377-380",
doi = "10.17796/jcpd.37.4.l135531h4542gj66"
}

Daković, D., Čolić, M., Čakić, S., Mileusnić, I., Hajduković, Z.,& Stamatović, N.. (2013). Salivary interleukin-8 levels in children suffering from Type 1 diabetes mellitus. in Journal of Clinical Pediatric Dentistry
Journal Pedodontics Inc, Birmingham., 37(4), 377-380.
https://doi.org/10.17796/jcpd.37.4.l135531h4542gj66

Daković D, Čolić M, Čakić S, Mileusnić I, Hajduković Z, Stamatović N. Salivary interleukin-8 levels in children suffering from Type 1 diabetes mellitus. in Journal of Clinical Pediatric Dentistry. 2013;37(4):377-380.
doi:10.17796/jcpd.37.4.l135531h4542gj66 .

Daković, Dragana, Čolić, Miodrag, Čakić, Saša, Mileusnić, Ivan, Hajduković, Zoran, Stamatović, Novak, "Salivary interleukin-8 levels in children suffering from Type 1 diabetes mellitus" in Journal of Clinical Pediatric Dentistry, 37, no. 4 (2013):377-380,
https://doi.org/10.17796/jcpd.37.4.l135531h4542gj66 . .

TY  - JOUR
AU  - Stanković-Popović, Verica
AU  - Popović, Dragan
AU  - Dimković, Nada
AU  - Maksić, Đoko
AU  - Vasilijić, Saša
AU  - Čolić, Miodrag
AU  - Vučinić, Žarko
AU  - Rađen, Slavica
AU  - Miličić, Biljana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1774
AB  - Background/Aim. Morbidity and mortality of continous ambulatory peritoneal dialysis (CAPD) patients is still very high. The aim of the study was to evaluate the effects of peritoneal dialysis (PD) solutions (standard vs biocompatible) on long-term patients' and the techique survival. Methods. A total of 42 stable patients on CAPD participated in this crosssectional study. They were prospectively followed-up during the twelve years. Patients with severe anemia (Hb  lt  10 g/L) and malignant disease ware excluded. Twenty one (50%) patients were treated with the standard PD solutions (CAPDP- 1) while the other 21 (50%) were treated with biocompatible PD solutions [(lower level of glucose degradation products, lower concentration of Ca2+ and neutral pH (CAPDP-2)]. All patients were analyzed for a presence of vascular calcification, nutrition status, and parameters of inflammation after 2.5 ± 0.6 years of starting CAPD, and these variables considered in the analysis as risk factors. Results. The patients from the group CAPDP-2 compared to those from the group CAPDP-1 had lower level of high-sensitivity C-reactive protein (hs-CRP) (p = 0.003), and better nutritional status as confirmed by the mid-arm circumference (p = 0.015), and midarm muscle circumference (p = 0.002) and subjective global assessment (p = 0.000). Also, they had lower vascular calcifications as confirmed by intima media thickness (IMT) (p = 0.003), degree of carotid narrowing (p = 0.001) and calcified plaques of common carotid arteries (CCA) (p = 0.008). Kaplan- Meier analysis confirmed better survival of patients from the group CAPDP-2 than those from the group CAPDP-1 (1-, 5-, and 10-year patients survival rate was: 100%, 61.9% and 14.3% for the group CAPDP-1, and 100%, 85.7%, and 52.4% for the group CAPDP-2, respectively; p = 0.0345). The 1-, 5-, and 10-year technique survival rate was: 100%, 71.4%, and 38.1% for the group CAPDP-1, and 100%, 85.7%, and 76.2% for the group CAPDP-2, respectively; (p = 0.0719). Duration of dialysis, serum triglyceride and cardiovascular score (quantitative scoring system consisting of: ejection fraction (EF) of left ventricle  lt  50%; IMT > 1 mm; carotid narrowing degree > 50%, presence of carotid plaques in both common carotide, ischaemic heart disease, cerebrovascular event and peripheral vascular disease with or without amputation) were independent predictors of overall patient survival. Duration of dialysis was only independent predictor of overall technique survival. Conclusion. Although patients treated with biocompatible solutions showed significantly better survival, the role of biocompatibility of CAPD solutions in patients and technique survival have to be confirmed. Namely, multivariate analysis confirmed that duration of dialysis, serum triglyceride and cardiovascular score significantly predicted overall CAPD patients survival, while only duration of dialysis was found to be independent predictor of overall technique survival.
AB  - Uvod/Cilj. Morbiditet i mortalitet bolesnika na kontinuiranoj ambulantnoj peritoneumskoj dijalizi (KAPD) i dalje je neprihvatljivo visok. Cilj rada bio je da se proceni uticaj vrste dijaliznih rasvora (bioinkompatibilni vs biokompatibilni) na višegodišnje preživljavanje bolesnika i same tehnike KAPD. Metode. Ovom studijom preseka sa delimično prospektivnim praćenjem ishoda lečenja obuhvaćeno je ukupno 42 nasumice izabrana, stabilna bolesnika (26 muškaraca i 16 žena) lečena primenom metode KAPD tokom poslednjih 12 godina. Isključeni su bolesnici sa teškom anemijom (Hb  lt 10 g/L) i malignom bolešću. Pri tome, 21 (50%) bolesnika kontinuirano je lečeno bioinkompatibilnim rastvorom za KAPD (kiseli standardni rastvor - ANDY-disc; grupa KAPDB-1), dok je preostalih 21 bolesnik sve vreme bilo na biokompatibilnijem rastvoru za KAPD (neutralni rastvor sa znatno manjom koncentracijom degradacionih produkata glukoze, 1.25 mmol/L Ca i 40 mmol/L laktata - Gambrosol Trio; grupa KAPDB-2). Svim bolesnicima određeni su odabrani parametri hronične inflamacije, malnutricije i ateroskleroze zajedno sa transportnim karakteristikama peritoneumske membrane i rezidualnom bubrežnom funkcijom nakon 2,5 ± 0,6 god od započinjanja KAPD. Svi dobijeni rezultati analizirani su kao potencijalni faktori rizika. Rezultati. Grupa KAPDB-2 u odnosu na KAPDB-1 imala je statistički značajno niže vrednosti serumskog hs-CRP (p = 0,003) i bolje parametre nutritivnog statusa izražene kroz obim nadlaktice (p = 0,015), obim mišića nadlaktice (p = 0,002) i subjektivnu globalnu procenu (p = 0,000) kao i u manjoj meri prisutnu aterosklerozu potvrđeno debljinom intimomedijalnog kompleksa (IMT) (p = 0,003), stepenom suženja karotida (p = 0,001) i prisustvom kalcifikovanih ateromatoznih plakova na karotidnim arterijama (p = 0,008). Kaplan-Meier-ova kriva preživljavanja potvrdila je značajno duže preživljavanje bolesnika u grupi KAPD-2 u odnosu na KAPDB-1 (1-, 5-, i 10-godišnje preživljavanje bolesnika iznosilo je redom: 100%, 61.9% i 14.3% u KAPDB-1, a 100%, 85,7% i 52,4% u KAPDB-2 grupi; p = 0,0345). Stopa 1-, 5-, i 10-godišnjeg preživljavanja metode iznosila je: 100%, 71,4% i 38,1% u KAPDB-1, a 100%, 85,7% i 76,2% u KAPDB-2 grupi (p = 0,0719). Kao nezavisni prediktori opšteg preživljavanja bolesnika na KAPD izdvojili su se: dijalizni staž, nivo serumskih triglicerida i skor kardiovaskularnog morbiditeta (kvantitativni sistem zbrajanja prisutnih sledećih parametara: ejekciona frakcija (EF) leve komore  lt  50%; IMT >1 mm; suženje lumena karotida > 50%; kalcifikovani ateromatozni plakovi na obe karotide; ishemijska bolest srca; cerebrovaskularni događaj i periferna vaskularna bolest sa ili bez gangrene). Kao nezavisan prediktor preživljavanja metode izdvojio se jedino dijalizni staž. Zaključak. Iako su bolesnici na KAPD sa biokompatibilnijim rastvorima pokazali statistički značajno bolje preživljavanje, ne možemo tvrditi da bioinkompatibilnost dijaliznih rastvora predstavlja značajan faktor rizika od preživljavanja bolesnika i same metode lečenja. Naime, multivarijantnom analizom kao prediktori opšteg preživljavanja bolesnika izdvojili su se samo dijalizni staž, nivo serumskih triglicerida i skor kardiovaskularnog morbiditeta, dok se za očuvanje peritoneumske membrane kao nezavisan faktor rizika prikazao samo dijalizni staž.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Influence of peritoneal dialysis solution biocompatibility on long-term survival of patients on continuous ambulatory peritoneal dialysis and the technique itself
T1  - Uticaj biokompatibilnosti rastvora za peritoneumsku dijalizu na višegodišnje preživljavanje bolesnika na kontinuiranoj ambulantnoj peritoneumskoj dijalizi i same metode lečenja
VL  - 70
IS  - 4
SP  - 352
EP  - 362
DO  - 10.2298/VSP1304352S
ER  - 

@article{
author = "Stanković-Popović, Verica and Popović, Dragan and Dimković, Nada and Maksić, Đoko and Vasilijić, Saša and Čolić, Miodrag and Vučinić, Žarko and Rađen, Slavica and Miličić, Biljana",
year = "2013",
abstract = "Background/Aim. Morbidity and mortality of continous ambulatory peritoneal dialysis (CAPD) patients is still very high. The aim of the study was to evaluate the effects of peritoneal dialysis (PD) solutions (standard vs biocompatible) on long-term patients' and the techique survival. Methods. A total of 42 stable patients on CAPD participated in this crosssectional study. They were prospectively followed-up during the twelve years. Patients with severe anemia (Hb  lt  10 g/L) and malignant disease ware excluded. Twenty one (50%) patients were treated with the standard PD solutions (CAPDP- 1) while the other 21 (50%) were treated with biocompatible PD solutions [(lower level of glucose degradation products, lower concentration of Ca2+ and neutral pH (CAPDP-2)]. All patients were analyzed for a presence of vascular calcification, nutrition status, and parameters of inflammation after 2.5 ± 0.6 years of starting CAPD, and these variables considered in the analysis as risk factors. Results. The patients from the group CAPDP-2 compared to those from the group CAPDP-1 had lower level of high-sensitivity C-reactive protein (hs-CRP) (p = 0.003), and better nutritional status as confirmed by the mid-arm circumference (p = 0.015), and midarm muscle circumference (p = 0.002) and subjective global assessment (p = 0.000). Also, they had lower vascular calcifications as confirmed by intima media thickness (IMT) (p = 0.003), degree of carotid narrowing (p = 0.001) and calcified plaques of common carotid arteries (CCA) (p = 0.008). Kaplan- Meier analysis confirmed better survival of patients from the group CAPDP-2 than those from the group CAPDP-1 (1-, 5-, and 10-year patients survival rate was: 100%, 61.9% and 14.3% for the group CAPDP-1, and 100%, 85.7%, and 52.4% for the group CAPDP-2, respectively; p = 0.0345). The 1-, 5-, and 10-year technique survival rate was: 100%, 71.4%, and 38.1% for the group CAPDP-1, and 100%, 85.7%, and 76.2% for the group CAPDP-2, respectively; (p = 0.0719). Duration of dialysis, serum triglyceride and cardiovascular score (quantitative scoring system consisting of: ejection fraction (EF) of left ventricle  lt  50%; IMT > 1 mm; carotid narrowing degree > 50%, presence of carotid plaques in both common carotide, ischaemic heart disease, cerebrovascular event and peripheral vascular disease with or without amputation) were independent predictors of overall patient survival. Duration of dialysis was only independent predictor of overall technique survival. Conclusion. Although patients treated with biocompatible solutions showed significantly better survival, the role of biocompatibility of CAPD solutions in patients and technique survival have to be confirmed. Namely, multivariate analysis confirmed that duration of dialysis, serum triglyceride and cardiovascular score significantly predicted overall CAPD patients survival, while only duration of dialysis was found to be independent predictor of overall technique survival., Uvod/Cilj. Morbiditet i mortalitet bolesnika na kontinuiranoj ambulantnoj peritoneumskoj dijalizi (KAPD) i dalje je neprihvatljivo visok. Cilj rada bio je da se proceni uticaj vrste dijaliznih rasvora (bioinkompatibilni vs biokompatibilni) na višegodišnje preživljavanje bolesnika i same tehnike KAPD. Metode. Ovom studijom preseka sa delimično prospektivnim praćenjem ishoda lečenja obuhvaćeno je ukupno 42 nasumice izabrana, stabilna bolesnika (26 muškaraca i 16 žena) lečena primenom metode KAPD tokom poslednjih 12 godina. Isključeni su bolesnici sa teškom anemijom (Hb  lt 10 g/L) i malignom bolešću. Pri tome, 21 (50%) bolesnika kontinuirano je lečeno bioinkompatibilnim rastvorom za KAPD (kiseli standardni rastvor - ANDY-disc; grupa KAPDB-1), dok je preostalih 21 bolesnik sve vreme bilo na biokompatibilnijem rastvoru za KAPD (neutralni rastvor sa znatno manjom koncentracijom degradacionih produkata glukoze, 1.25 mmol/L Ca i 40 mmol/L laktata - Gambrosol Trio; grupa KAPDB-2). Svim bolesnicima određeni su odabrani parametri hronične inflamacije, malnutricije i ateroskleroze zajedno sa transportnim karakteristikama peritoneumske membrane i rezidualnom bubrežnom funkcijom nakon 2,5 ± 0,6 god od započinjanja KAPD. Svi dobijeni rezultati analizirani su kao potencijalni faktori rizika. Rezultati. Grupa KAPDB-2 u odnosu na KAPDB-1 imala je statistički značajno niže vrednosti serumskog hs-CRP (p = 0,003) i bolje parametre nutritivnog statusa izražene kroz obim nadlaktice (p = 0,015), obim mišića nadlaktice (p = 0,002) i subjektivnu globalnu procenu (p = 0,000) kao i u manjoj meri prisutnu aterosklerozu potvrđeno debljinom intimomedijalnog kompleksa (IMT) (p = 0,003), stepenom suženja karotida (p = 0,001) i prisustvom kalcifikovanih ateromatoznih plakova na karotidnim arterijama (p = 0,008). Kaplan-Meier-ova kriva preživljavanja potvrdila je značajno duže preživljavanje bolesnika u grupi KAPD-2 u odnosu na KAPDB-1 (1-, 5-, i 10-godišnje preživljavanje bolesnika iznosilo je redom: 100%, 61.9% i 14.3% u KAPDB-1, a 100%, 85,7% i 52,4% u KAPDB-2 grupi; p = 0,0345). Stopa 1-, 5-, i 10-godišnjeg preživljavanja metode iznosila je: 100%, 71,4% i 38,1% u KAPDB-1, a 100%, 85,7% i 76,2% u KAPDB-2 grupi (p = 0,0719). Kao nezavisni prediktori opšteg preživljavanja bolesnika na KAPD izdvojili su se: dijalizni staž, nivo serumskih triglicerida i skor kardiovaskularnog morbiditeta (kvantitativni sistem zbrajanja prisutnih sledećih parametara: ejekciona frakcija (EF) leve komore  lt  50%; IMT >1 mm; suženje lumena karotida > 50%; kalcifikovani ateromatozni plakovi na obe karotide; ishemijska bolest srca; cerebrovaskularni događaj i periferna vaskularna bolest sa ili bez gangrene). Kao nezavisan prediktor preživljavanja metode izdvojio se jedino dijalizni staž. Zaključak. Iako su bolesnici na KAPD sa biokompatibilnijim rastvorima pokazali statistički značajno bolje preživljavanje, ne možemo tvrditi da bioinkompatibilnost dijaliznih rastvora predstavlja značajan faktor rizika od preživljavanja bolesnika i same metode lečenja. Naime, multivarijantnom analizom kao prediktori opšteg preživljavanja bolesnika izdvojili su se samo dijalizni staž, nivo serumskih triglicerida i skor kardiovaskularnog morbiditeta, dok se za očuvanje peritoneumske membrane kao nezavisan faktor rizika prikazao samo dijalizni staž.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Influence of peritoneal dialysis solution biocompatibility on long-term survival of patients on continuous ambulatory peritoneal dialysis and the technique itself, Uticaj biokompatibilnosti rastvora za peritoneumsku dijalizu na višegodišnje preživljavanje bolesnika na kontinuiranoj ambulantnoj peritoneumskoj dijalizi i same metode lečenja",
volume = "70",
number = "4",
pages = "352-362",
doi = "10.2298/VSP1304352S"
}

Stanković-Popović, V., Popović, D., Dimković, N., Maksić, Đ., Vasilijić, S., Čolić, M., Vučinić, Ž., Rađen, S.,& Miličić, B.. (2013). Influence of peritoneal dialysis solution biocompatibility on long-term survival of patients on continuous ambulatory peritoneal dialysis and the technique itself. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 70(4), 352-362.
https://doi.org/10.2298/VSP1304352S

Stanković-Popović V, Popović D, Dimković N, Maksić Đ, Vasilijić S, Čolić M, Vučinić Ž, Rađen S, Miličić B. Influence of peritoneal dialysis solution biocompatibility on long-term survival of patients on continuous ambulatory peritoneal dialysis and the technique itself. in Vojnosanitetski pregled. 2013;70(4):352-362.
doi:10.2298/VSP1304352S .

Stanković-Popović, Verica, Popović, Dragan, Dimković, Nada, Maksić, Đoko, Vasilijić, Saša, Čolić, Miodrag, Vučinić, Žarko, Rađen, Slavica, Miličić, Biljana, "Influence of peritoneal dialysis solution biocompatibility on long-term survival of patients on continuous ambulatory peritoneal dialysis and the technique itself" in Vojnosanitetski pregled, 70, no. 4 (2013):352-362,
https://doi.org/10.2298/VSP1304352S . .

TY  - JOUR
AU  - Dožić, Ivan
AU  - Todorović, Tatjana
AU  - Čolić, Miodrag
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1703
AB  - Introduction. Submandibular salivary gland is a part of the neuro-immune-endocrine system. It contains biological factors which regulate a number of functions in the body including the modulation of thymus function. Objective. The aim of the study was to investigate immunophenotypic characteristics of submandibular salivary glands of rats during ontogenesis, using the panels of monoclonal antibodies and to compare with the phenotypic characteristics of epithelial components of the thymus. Methods. Submandibular salivary glands and thymus were obtained from 1, 30 and 60 days old male AO (Albino, Oxford) rats. Streptavidin-biotin peroxidase method was used for staining. Results. Immunohistochemical analysis of rat submandibular salivary glands showed phenotypic heterogeneity of particular components of this gland during the postnatal development. We demonstrated that rat submandibular salivary glands share common antigens with rat thymic epithelial cells, but the observed phenotypic similarity between the individual regions was considered much more significant. Our data showed that the phenotypic similarity between duct epithelial cells and subcapsular epithelial cells and most medullary cells, whereas cortical epithelial cells are phenotypically similar to acinar cells. Conclusion. This immunohistological study showed phenotypic complexity of the submandibular salivary gland and similarity to the thymus that opens new perspectives in studying phenotypic similarities between this gland and lymphatic organs.
AB  - Uvod. Submandibularna pljuvačna žlezda je deo neuroimunoendokrinog sistema koja posredstvom bioloških faktora reguliše mnoge funkcije u organizmu, uključujući i modulaciju funkcije timusa. Cilj rada. Cilj rada bio je da se ispitaju imunofenotipske odlike submandibularne pljuvačne žlezde pacova tokom ontogeneze pomoću panela monoklonskih antitela i uporede s fenotipskim svojstvima epitelnih komponenti timusa. Metode rada. Istraživanja su rađena na pacovima soja AO (Albino Oxford), muškog pola, starosti od jednog, 30 i 60 dana. Za fenotipsku analizu korišćena je streptavidin-biotin- peroksidazna metoda bojenja. Rezultati. Ova imunohistološka studija ukazuje na heterogenost fenotipskih odlika pojedinih komponenti submandibularne pljuvačne žlezde tokom postnatalnog razvoja. Takođe je ustanovljeno da submandibularna pljuvačna žlezda i timus imaju zajedničke antigene, ali je mnogo značajnija fenotipska sličnost između pojedinih regiona u oba ispitivana organa. Epitelne ćelije izvodnih kanala submandibularne pljuvačne žlezde su fenotipski slične epitelnim ćelijama subkapsularne i većini ćelija u meduli timusa. Epitelne ćelije acinusa su fenotipski slične epitelnim ćelijama korteksa timusa. Zaključak. Fenotipska kompleksnost submandibularne pljuvačne žlezde i sličnost s timusom otvara nove mogućnosti za analizu fenotipske sličnosti između ove žlezde i limfatičnih organa.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Common immunophenotypic features of submandibular salivary glands and thymus in rats
T1  - Zajedničke imunofenotipske odlike podvilične pljuvačne žlezde i grudne žlezde pacova
VL  - 140
IS  - 5-6
SP  - 270
EP  - 277
DO  - 10.2298/SARH1206270D
ER  - 

@article{
author = "Dožić, Ivan and Todorović, Tatjana and Čolić, Miodrag",
year = "2012",
abstract = "Introduction. Submandibular salivary gland is a part of the neuro-immune-endocrine system. It contains biological factors which regulate a number of functions in the body including the modulation of thymus function. Objective. The aim of the study was to investigate immunophenotypic characteristics of submandibular salivary glands of rats during ontogenesis, using the panels of monoclonal antibodies and to compare with the phenotypic characteristics of epithelial components of the thymus. Methods. Submandibular salivary glands and thymus were obtained from 1, 30 and 60 days old male AO (Albino, Oxford) rats. Streptavidin-biotin peroxidase method was used for staining. Results. Immunohistochemical analysis of rat submandibular salivary glands showed phenotypic heterogeneity of particular components of this gland during the postnatal development. We demonstrated that rat submandibular salivary glands share common antigens with rat thymic epithelial cells, but the observed phenotypic similarity between the individual regions was considered much more significant. Our data showed that the phenotypic similarity between duct epithelial cells and subcapsular epithelial cells and most medullary cells, whereas cortical epithelial cells are phenotypically similar to acinar cells. Conclusion. This immunohistological study showed phenotypic complexity of the submandibular salivary gland and similarity to the thymus that opens new perspectives in studying phenotypic similarities between this gland and lymphatic organs., Uvod. Submandibularna pljuvačna žlezda je deo neuroimunoendokrinog sistema koja posredstvom bioloških faktora reguliše mnoge funkcije u organizmu, uključujući i modulaciju funkcije timusa. Cilj rada. Cilj rada bio je da se ispitaju imunofenotipske odlike submandibularne pljuvačne žlezde pacova tokom ontogeneze pomoću panela monoklonskih antitela i uporede s fenotipskim svojstvima epitelnih komponenti timusa. Metode rada. Istraživanja su rađena na pacovima soja AO (Albino Oxford), muškog pola, starosti od jednog, 30 i 60 dana. Za fenotipsku analizu korišćena je streptavidin-biotin- peroksidazna metoda bojenja. Rezultati. Ova imunohistološka studija ukazuje na heterogenost fenotipskih odlika pojedinih komponenti submandibularne pljuvačne žlezde tokom postnatalnog razvoja. Takođe je ustanovljeno da submandibularna pljuvačna žlezda i timus imaju zajedničke antigene, ali je mnogo značajnija fenotipska sličnost između pojedinih regiona u oba ispitivana organa. Epitelne ćelije izvodnih kanala submandibularne pljuvačne žlezde su fenotipski slične epitelnim ćelijama subkapsularne i većini ćelija u meduli timusa. Epitelne ćelije acinusa su fenotipski slične epitelnim ćelijama korteksa timusa. Zaključak. Fenotipska kompleksnost submandibularne pljuvačne žlezde i sličnost s timusom otvara nove mogućnosti za analizu fenotipske sličnosti između ove žlezde i limfatičnih organa.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Common immunophenotypic features of submandibular salivary glands and thymus in rats, Zajedničke imunofenotipske odlike podvilične pljuvačne žlezde i grudne žlezde pacova",
volume = "140",
number = "5-6",
pages = "270-277",
doi = "10.2298/SARH1206270D"
}

Dožić, I., Todorović, T.,& Čolić, M.. (2012). Common immunophenotypic features of submandibular salivary glands and thymus in rats. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 140(5-6), 270-277.
https://doi.org/10.2298/SARH1206270D

Dožić I, Todorović T, Čolić M. Common immunophenotypic features of submandibular salivary glands and thymus in rats. in Srpski arhiv za celokupno lekarstvo. 2012;140(5-6):270-277.
doi:10.2298/SARH1206270D .

Dožić, Ivan, Todorović, Tatjana, Čolić, Miodrag, "Common immunophenotypic features of submandibular salivary glands and thymus in rats" in Srpski arhiv za celokupno lekarstvo, 140, no. 5-6 (2012):270-277,
https://doi.org/10.2298/SARH1206270D . .

TY  - JOUR
AU  - Rudolf, Rebeka
AU  - Anzel, M.
AU  - Marković, Evgenija
AU  - Čolić, Miodrag
AU  - Stamenković, D.
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1688
AB  - This paper deals with gold, which is described as a chemical element. Special attention is paid to its physical-chemical properties and, furthermore, where or in what form it can be found in nature. We discuss the role it has played through history and we inform how gold has been developed to the level it has reached today's value. Still more, when gold is broken into nanoparticles, this form could be highly useful for a wide range of processes, including general nanotechnology, electronics manufacturing and the synthesizing of different functional materials. It is important that we know that gold is also used in industry in many engineering applications (contacts in micro-electronics) and medicine (dental alloys, implants).
PB  - Croatian Metallurgical Society
T2  - Metalurgija
T1  - Gold in the past, today and future
VL  - 51
IS  - 2
SP  - 261
EP  - 264
UR  - https://hdl.handle.net/21.15107/rcub_smile_1688
ER  - 

@article{
author = "Rudolf, Rebeka and Anzel, M. and Marković, Evgenija and Čolić, Miodrag and Stamenković, D.",
year = "2012",
abstract = "This paper deals with gold, which is described as a chemical element. Special attention is paid to its physical-chemical properties and, furthermore, where or in what form it can be found in nature. We discuss the role it has played through history and we inform how gold has been developed to the level it has reached today's value. Still more, when gold is broken into nanoparticles, this form could be highly useful for a wide range of processes, including general nanotechnology, electronics manufacturing and the synthesizing of different functional materials. It is important that we know that gold is also used in industry in many engineering applications (contacts in micro-electronics) and medicine (dental alloys, implants).",
publisher = "Croatian Metallurgical Society",
journal = "Metalurgija",
title = "Gold in the past, today and future",
volume = "51",
number = "2",
pages = "261-264",
url = "https://hdl.handle.net/21.15107/rcub_smile_1688"
}

Rudolf, R., Anzel, M., Marković, E., Čolić, M.,& Stamenković, D.. (2012). Gold in the past, today and future. in Metalurgija
Croatian Metallurgical Society., 51(2), 261-264.
https://hdl.handle.net/21.15107/rcub_smile_1688

Rudolf R, Anzel M, Marković E, Čolić M, Stamenković D. Gold in the past, today and future. in Metalurgija. 2012;51(2):261-264.
https://hdl.handle.net/21.15107/rcub_smile_1688 .

Rudolf, Rebeka, Anzel, M., Marković, Evgenija, Čolić, Miodrag, Stamenković, D., "Gold in the past, today and future" in Metalurgija, 51, no. 2 (2012):261-264,
https://hdl.handle.net/21.15107/rcub_smile_1688 .

TY  - JOUR
AU  - Stanković-Popović, Verica
AU  - Nesić, V.
AU  - Popović, D.
AU  - Maksić, Đoko
AU  - Čolić, Miodrag
AU  - Vasilijić, Saša
AU  - Vučinić, Žarko
AU  - Miličić, Biljana
AU  - Rađen, Slavica
AU  - Dimković, Nada
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1614
AB  - Background: Chronic inflammation, malnutrition and atherosclerosis (MIA syndrome) are important predictors of high mortality in continuous ambulatory peritoneal dialysis (CAPD) patients. We aimed to evaluate the effects of PD solutions (standard vs. biocompatible) on some parameters of MIA syndrome in patients undergoing CAPD. Methods: 42 stable patients who were on CAPD at least 2.5 years participated in this cross-sectional study. Patients who had severe anemia (Hb  lt  10 g/l), immunomodulatory therapy, peritonitis or any inflammatory conditions for at least 3 months before the analysis, malignant disease and acute exacerbation of heart failure, were excluded. 21 (50%) patients were treated with standard PD solutions (CAPDP-1), while the remaining 21(50% of patients) were treated with biocompatible PD solutions (neutral solutions with lower level of glucose degradation products and lower concentration of calcium, CAPDP-2). All patients underwent echocardiography and B-mode ultrasonography of common carotid arteries together with assessments of nutrition status and parameters of systemic and local inflammation. Results: There were no significant differences between the groups concerning age, gender, underlying disease, residual renal function, peritoneal transport characteristics, comorbidity or therapy applied. Patients from group CAPDP-2 had a significantly lower serum level of hs-CRP (3.7 +/- 2.6 mg/l vs. 6.3 +/- 4.5 mg/l; p = 0.023) and significantly better nutritional status confirmed by mid-arm circumference (p = 0.015), mid-arm muscle circumference (p = 0.002) and subjective global assessment (14.28% of patients in CAPDP-2 vs. 71% of patients in CAPDP-1 were malnourished; p = 0.000). Group CAPD-2 had less frequent left ventricular hypertrophy (p = 0.039), thinner intima-media thickness (p = 0.005), smaller carotid narrowing (p = 0.000) and fewer calcified plaques of common carotide arteries (p = 0.003). No significant difference between the CAPDP groups was observed in serum and effluent levels of inflammatory cytokines (IL-1, IL-6 and TNF-alpha) and CA-125 effluent level. Logistic regression analysis did not confirm that biocompatibility of PD solutions was an independent predictor of any parameter of MIA syndrome. Conclusions: According to the present study and logistic regression analysis, the effect of biocompatible CAPD solutions on parameters of malnutrition, inflammation and atherosclerosis have to be confirmed by well-designed and controlled studies in a higher number of patients.
PB  - Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen
T2  - Clinical Nephrology
T1  - Effects of conventional versus biocompatible peritoneal dialysis solutions on peritoneal and systemic inflammation, malnutrition and atherosclerosis in CAPD patients
VL  - 76
IS  - 4
SP  - 314
EP  - 322
DO  - 10.5414/CN106991
ER  - 

@article{
author = "Stanković-Popović, Verica and Nesić, V. and Popović, D. and Maksić, Đoko and Čolić, Miodrag and Vasilijić, Saša and Vučinić, Žarko and Miličić, Biljana and Rađen, Slavica and Dimković, Nada",
year = "2011",
abstract = "Background: Chronic inflammation, malnutrition and atherosclerosis (MIA syndrome) are important predictors of high mortality in continuous ambulatory peritoneal dialysis (CAPD) patients. We aimed to evaluate the effects of PD solutions (standard vs. biocompatible) on some parameters of MIA syndrome in patients undergoing CAPD. Methods: 42 stable patients who were on CAPD at least 2.5 years participated in this cross-sectional study. Patients who had severe anemia (Hb  lt  10 g/l), immunomodulatory therapy, peritonitis or any inflammatory conditions for at least 3 months before the analysis, malignant disease and acute exacerbation of heart failure, were excluded. 21 (50%) patients were treated with standard PD solutions (CAPDP-1), while the remaining 21(50% of patients) were treated with biocompatible PD solutions (neutral solutions with lower level of glucose degradation products and lower concentration of calcium, CAPDP-2). All patients underwent echocardiography and B-mode ultrasonography of common carotid arteries together with assessments of nutrition status and parameters of systemic and local inflammation. Results: There were no significant differences between the groups concerning age, gender, underlying disease, residual renal function, peritoneal transport characteristics, comorbidity or therapy applied. Patients from group CAPDP-2 had a significantly lower serum level of hs-CRP (3.7 +/- 2.6 mg/l vs. 6.3 +/- 4.5 mg/l; p = 0.023) and significantly better nutritional status confirmed by mid-arm circumference (p = 0.015), mid-arm muscle circumference (p = 0.002) and subjective global assessment (14.28% of patients in CAPDP-2 vs. 71% of patients in CAPDP-1 were malnourished; p = 0.000). Group CAPD-2 had less frequent left ventricular hypertrophy (p = 0.039), thinner intima-media thickness (p = 0.005), smaller carotid narrowing (p = 0.000) and fewer calcified plaques of common carotide arteries (p = 0.003). No significant difference between the CAPDP groups was observed in serum and effluent levels of inflammatory cytokines (IL-1, IL-6 and TNF-alpha) and CA-125 effluent level. Logistic regression analysis did not confirm that biocompatibility of PD solutions was an independent predictor of any parameter of MIA syndrome. Conclusions: According to the present study and logistic regression analysis, the effect of biocompatible CAPD solutions on parameters of malnutrition, inflammation and atherosclerosis have to be confirmed by well-designed and controlled studies in a higher number of patients.",
publisher = "Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen",
journal = "Clinical Nephrology",
title = "Effects of conventional versus biocompatible peritoneal dialysis solutions on peritoneal and systemic inflammation, malnutrition and atherosclerosis in CAPD patients",
volume = "76",
number = "4",
pages = "314-322",
doi = "10.5414/CN106991"
}

Stanković-Popović, V., Nesić, V., Popović, D., Maksić, Đ., Čolić, M., Vasilijić, S., Vučinić, Ž., Miličić, B., Rađen, S.,& Dimković, N.. (2011). Effects of conventional versus biocompatible peritoneal dialysis solutions on peritoneal and systemic inflammation, malnutrition and atherosclerosis in CAPD patients. in Clinical Nephrology
Dustri-Verlag Dr Karl Feistle, Deisenhofen-Muenchen., 76(4), 314-322.
https://doi.org/10.5414/CN106991

Stanković-Popović V, Nesić V, Popović D, Maksić Đ, Čolić M, Vasilijić S, Vučinić Ž, Miličić B, Rađen S, Dimković N. Effects of conventional versus biocompatible peritoneal dialysis solutions on peritoneal and systemic inflammation, malnutrition and atherosclerosis in CAPD patients. in Clinical Nephrology. 2011;76(4):314-322.
doi:10.5414/CN106991 .

Stanković-Popović, Verica, Nesić, V., Popović, D., Maksić, Đoko, Čolić, Miodrag, Vasilijić, Saša, Vučinić, Žarko, Miličić, Biljana, Rađen, Slavica, Dimković, Nada, "Effects of conventional versus biocompatible peritoneal dialysis solutions on peritoneal and systemic inflammation, malnutrition and atherosclerosis in CAPD patients" in Clinical Nephrology, 76, no. 4 (2011):314-322,
https://doi.org/10.5414/CN106991 . .

TY  - JOUR
AU  - Rudolf, Rebeka
AU  - Anžel, Ivan
AU  - Gusel, L.
AU  - Stamenković, D.
AU  - Todorović, A.
AU  - Čolić, Miodrag
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1579
AB  - Microstructures of two high noble experimental Au-Pt alloys were compared before and after conditioning for biocompatibility, in order to identify phases and microelements responsible for the alloys' corrosive behaviour. Microstructural characterization was carried-out by optical and scanning electron microscopy, in addition to energy dispersive X-ray analysis. X-ray diffraction was applied to determine the phases' composition and their contribution in the alloys. Additionally, simultaneous thermal analysis was used to identify the temperatures of phase transformations. An overall assessment before conditioning showed that Au-Pt I is a two-phase alloy containing a dominant Au-rich alpha(1) phase and a minor Pt-rich alpha(2) phase, while the Au-Pt II alloy contains in addition three minor phases: AuZn3, Pt3Zn and Au1.4Zn0.52. The highest content of Zn (up to 6.76 wt.%) was detected in the Pt3Zn phase. After RPMI cell culture medium conditioning, the Pt3Zn and AuZn3 phases disappeared, suggesting that they are predominantly responsible for Zn loss and the lower corrosive stability of the Au-Pt II alloy.
PB  - Korean Inst Metals Materials, Seoul
T2  - Metals & Materials International
T1  - Microstructural analyses of two high noble gold-platinum alloys before and after conditioning in a cell culture medium
VL  - 16
IS  - 6
SP  - 931
EP  - 940
DO  - 10.1007/s12540-010-1211-4
ER  - 

@article{
author = "Rudolf, Rebeka and Anžel, Ivan and Gusel, L. and Stamenković, D. and Todorović, A. and Čolić, Miodrag",
year = "2010",
abstract = "Microstructures of two high noble experimental Au-Pt alloys were compared before and after conditioning for biocompatibility, in order to identify phases and microelements responsible for the alloys' corrosive behaviour. Microstructural characterization was carried-out by optical and scanning electron microscopy, in addition to energy dispersive X-ray analysis. X-ray diffraction was applied to determine the phases' composition and their contribution in the alloys. Additionally, simultaneous thermal analysis was used to identify the temperatures of phase transformations. An overall assessment before conditioning showed that Au-Pt I is a two-phase alloy containing a dominant Au-rich alpha(1) phase and a minor Pt-rich alpha(2) phase, while the Au-Pt II alloy contains in addition three minor phases: AuZn3, Pt3Zn and Au1.4Zn0.52. The highest content of Zn (up to 6.76 wt.%) was detected in the Pt3Zn phase. After RPMI cell culture medium conditioning, the Pt3Zn and AuZn3 phases disappeared, suggesting that they are predominantly responsible for Zn loss and the lower corrosive stability of the Au-Pt II alloy.",
publisher = "Korean Inst Metals Materials, Seoul",
journal = "Metals & Materials International",
title = "Microstructural analyses of two high noble gold-platinum alloys before and after conditioning in a cell culture medium",
volume = "16",
number = "6",
pages = "931-940",
doi = "10.1007/s12540-010-1211-4"
}

Rudolf, R., Anžel, I., Gusel, L., Stamenković, D., Todorović, A.,& Čolić, M.. (2010). Microstructural analyses of two high noble gold-platinum alloys before and after conditioning in a cell culture medium. in Metals & Materials International
Korean Inst Metals Materials, Seoul., 16(6), 931-940.
https://doi.org/10.1007/s12540-010-1211-4

Rudolf R, Anžel I, Gusel L, Stamenković D, Todorović A, Čolić M. Microstructural analyses of two high noble gold-platinum alloys before and after conditioning in a cell culture medium. in Metals & Materials International. 2010;16(6):931-940.
doi:10.1007/s12540-010-1211-4 .

Rudolf, Rebeka, Anžel, Ivan, Gusel, L., Stamenković, D., Todorović, A., Čolić, Miodrag, "Microstructural analyses of two high noble gold-platinum alloys before and after conditioning in a cell culture medium" in Metals & Materials International, 16, no. 6 (2010):931-940,
https://doi.org/10.1007/s12540-010-1211-4 . .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Rudolf, Rebeka
AU  - Stamenković, Dragoslav
AU  - Anžel, Ivan
AU  - Vucević, Dragana
AU  - Jenko, Monika
AU  - Lazić, Vojkan
AU  - Lojen, Gorazd
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1553
AB  - Cu-Al-Ni shape memory alloys (SMAs) have been investigated as materials for medical devices, but their biomedical application is still limited. The aim of this work was to compare the microstructure, corrosion and cytotoxicity in vitro of a Cu-Al-Ni SMA. Rapidly solidified (RS) thin ribbons, manufactured via melt spinning, were used for the tests. The control alloy was a permanent mould casting of the same composition, but without shape memory effect. The results show that RS ribbons are significantly more resistant to corrosion compared with the control alloy, as judged by the lesser release of Cu and Ni into the conditioning medium. These results correlate with the finding that RS ribbons were not cytotoxic to L929 mouse fibroblasts and rat thymocytes. In addition, the RS ribbon conditioning medium inhibited cellular proliferation and IL-2 production by activated rat splenocytes to a much lesser extent. The inhibitory effects were almost completely abolished by conditioning the RS ribbons in culture medium for 4 weeks. Microstructural analysis showed that RS ribbons are martensitic, with boron particles as a minor phase. In contrast, the control Cu-Al-Ni alloy had a complex multiphase microstructure. Examination of the alloy surfaces after conditioning by energy dispersive X-ray and Auger electron spectroscopy showed the formation of Cu and Al oxide layers and confirmed that the metals in RS ribbons are less susceptible to oxidation and corrosion compared with the control alloy. In conclusion, these results suggest that rapid solidification significantly improves the corrosion stability and biocompatibility in vitro of Cu-Al-Ni SMA ribbons.
PB  - Elsevier Sci Ltd, Oxford
T2  - Acta Biomaterialia
T1  - Relationship between microstructure, cytotoxicity and corrosion properties of a Cu-Al-Ni shape memory alloy
VL  - 6
IS  - 1
SP  - 308
EP  - 317
DO  - 10.1016/j.actbio.2009.06.027
ER  - 

@article{
author = "Čolić, Miodrag and Rudolf, Rebeka and Stamenković, Dragoslav and Anžel, Ivan and Vucević, Dragana and Jenko, Monika and Lazić, Vojkan and Lojen, Gorazd",
year = "2010",
abstract = "Cu-Al-Ni shape memory alloys (SMAs) have been investigated as materials for medical devices, but their biomedical application is still limited. The aim of this work was to compare the microstructure, corrosion and cytotoxicity in vitro of a Cu-Al-Ni SMA. Rapidly solidified (RS) thin ribbons, manufactured via melt spinning, were used for the tests. The control alloy was a permanent mould casting of the same composition, but without shape memory effect. The results show that RS ribbons are significantly more resistant to corrosion compared with the control alloy, as judged by the lesser release of Cu and Ni into the conditioning medium. These results correlate with the finding that RS ribbons were not cytotoxic to L929 mouse fibroblasts and rat thymocytes. In addition, the RS ribbon conditioning medium inhibited cellular proliferation and IL-2 production by activated rat splenocytes to a much lesser extent. The inhibitory effects were almost completely abolished by conditioning the RS ribbons in culture medium for 4 weeks. Microstructural analysis showed that RS ribbons are martensitic, with boron particles as a minor phase. In contrast, the control Cu-Al-Ni alloy had a complex multiphase microstructure. Examination of the alloy surfaces after conditioning by energy dispersive X-ray and Auger electron spectroscopy showed the formation of Cu and Al oxide layers and confirmed that the metals in RS ribbons are less susceptible to oxidation and corrosion compared with the control alloy. In conclusion, these results suggest that rapid solidification significantly improves the corrosion stability and biocompatibility in vitro of Cu-Al-Ni SMA ribbons.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Acta Biomaterialia",
title = "Relationship between microstructure, cytotoxicity and corrosion properties of a Cu-Al-Ni shape memory alloy",
volume = "6",
number = "1",
pages = "308-317",
doi = "10.1016/j.actbio.2009.06.027"
}

Čolić, M., Rudolf, R., Stamenković, D., Anžel, I., Vucević, D., Jenko, M., Lazić, V.,& Lojen, G.. (2010). Relationship between microstructure, cytotoxicity and corrosion properties of a Cu-Al-Ni shape memory alloy. in Acta Biomaterialia
Elsevier Sci Ltd, Oxford., 6(1), 308-317.
https://doi.org/10.1016/j.actbio.2009.06.027

Čolić M, Rudolf R, Stamenković D, Anžel I, Vucević D, Jenko M, Lazić V, Lojen G. Relationship between microstructure, cytotoxicity and corrosion properties of a Cu-Al-Ni shape memory alloy. in Acta Biomaterialia. 2010;6(1):308-317.
doi:10.1016/j.actbio.2009.06.027 .

Čolić, Miodrag, Rudolf, Rebeka, Stamenković, Dragoslav, Anžel, Ivan, Vucević, Dragana, Jenko, Monika, Lazić, Vojkan, Lojen, Gorazd, "Relationship between microstructure, cytotoxicity and corrosion properties of a Cu-Al-Ni shape memory alloy" in Acta Biomaterialia, 6, no. 1 (2010):308-317,
https://doi.org/10.1016/j.actbio.2009.06.027 . .

TY  - JOUR
AU  - Dožić, Ivan
AU  - Todorović, Tatjana
AU  - Dožić, Branko
AU  - Čolić, Miodrag
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1471
AB  - Cytokeratins (CK) are a group of proteins comprised of at least 20 different skeletal polypeptides specific for simple and complex epithelia of almost all tissues. Until recently only a few antibodies for cytoskeletal proteins were available and thus it was very difficult to visualize all the components of postnatal cytodifferentiation of salivary glands. Therefore, morphologic and immunophenotypic features of the CK polypeptides, have been analized within the rat submandibular salivary glands (SSG) during postnatal development. SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with monoclonal antibodies (mAbs) which specifically react with CK polypeptides (CK7, CK8, CK18, CK kidney, K8.12, K8.13) and rat epithelium (PT13D11). Immunohistochemical analysis of the rat SSG showed phenotypic heterogeneity of particular components of this gland during postnatal development. Regarding CK profiles it was shown that epithelial cells of the acini expressed CK18, whereas epithelial cells of the duct expressed K8.12 and K8.13 mAbs. Epithelial cells of the acini were not stained by K8.12 and PT13D11 mAbs. Intercalated ducts were strongly CK kidney+ and PT13D11+. Myoepithelial cells of the acini were stained by K8.13 mAbs, whereas these cells were not stained by mAbs specific for simple epithelia. This immunohistological study showed that the heterogeneity expression of CK polypeptides might also be useful to further understand the origin of epithelial SSG cells, as well as their development and function.
AB  - Citokeratini (CK) su grupa složenih proteina koji se sastoje od najmanje 20 različitih polipeptida citoskeleta, specifični za običan i složeni epitel skoro svih tkiva. Do skoro je bilo dostupno samo nekoliko antitela specifičnih za proteine citoskeleta i zbog toga je bila otežana vizualizacija komponenti postnatalne citodiferencijacije pljuvačnih žlezda. U ovoj studiji su, pomoću panela monoklonskih antitela (mAt), analizirane morfološke i imunofenotipske osobine CK polipeptida, u submandibularnoj pljuvač noj žlezdi (SPŽ) pacova tokom ontogeneze. Submandiblarne pljuvačne žlezde uzimane su od soja AO pacova, muškog pola, starosti 1, 30 i 60 dana. U ovom radu korišćena je imunohistohemijska metoda streptavidin-biotin peroksidaznog bojenja. Tkivni preseci fiksirani su u acetonu i inkubirani sa mAt specifičnim za CK polipeptide (CK7, CK 8, CK 18, CK bubrega, K8.12, K8.13) i epitel pacova (PT13D11). U ovoj imunohistohemijskoj analizi, ekspresija anti -CK antitela na SPŽ pacova, pokazuje fenotipsku heterogenost određenih komponenti ove žlezde tokom postnatalnog perioda. Epitelne ćelije acinusa su CK18+, dok epitelne ćelije kanalića imaju izražene CK definisane sa K8.12 i K8.13 mAt. Epitelne ćelije acinusa nisu bile obojene sa K8.12 i PT13D11 mAt. Umetnuti kanali su bili izrazito CK bubrega+ i PT13D11+. Mioepitelne ćelije acinusa su bile obojene sa K8.13 i PT13D11 mAt, dok su ove ćelije imale negativnu reakciju sa mAt specifičnim za obični epitel. Ova imunohistohemijska studija pokazuje heterogenost u ekspresiji CK polipeptida i može poslužiti za razumevanje porekla, razvoja i funkcije epitelnih ćelija SPŽ pacova.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis
T1  - Imunohistohemijska identifikacija citokeratina u submandibularnoj pljuvačnoj žlezdi pacova u toku ontogeneze
VL  - 59
IS  - 1
SP  - 69
EP  - 80
DO  - 10.2298/AVB0901069D
ER  - 

@article{
author = "Dožić, Ivan and Todorović, Tatjana and Dožić, Branko and Čolić, Miodrag",
year = "2009",
abstract = "Cytokeratins (CK) are a group of proteins comprised of at least 20 different skeletal polypeptides specific for simple and complex epithelia of almost all tissues. Until recently only a few antibodies for cytoskeletal proteins were available and thus it was very difficult to visualize all the components of postnatal cytodifferentiation of salivary glands. Therefore, morphologic and immunophenotypic features of the CK polypeptides, have been analized within the rat submandibular salivary glands (SSG) during postnatal development. SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with monoclonal antibodies (mAbs) which specifically react with CK polypeptides (CK7, CK8, CK18, CK kidney, K8.12, K8.13) and rat epithelium (PT13D11). Immunohistochemical analysis of the rat SSG showed phenotypic heterogeneity of particular components of this gland during postnatal development. Regarding CK profiles it was shown that epithelial cells of the acini expressed CK18, whereas epithelial cells of the duct expressed K8.12 and K8.13 mAbs. Epithelial cells of the acini were not stained by K8.12 and PT13D11 mAbs. Intercalated ducts were strongly CK kidney+ and PT13D11+. Myoepithelial cells of the acini were stained by K8.13 mAbs, whereas these cells were not stained by mAbs specific for simple epithelia. This immunohistological study showed that the heterogeneity expression of CK polypeptides might also be useful to further understand the origin of epithelial SSG cells, as well as their development and function., Citokeratini (CK) su grupa složenih proteina koji se sastoje od najmanje 20 različitih polipeptida citoskeleta, specifični za običan i složeni epitel skoro svih tkiva. Do skoro je bilo dostupno samo nekoliko antitela specifičnih za proteine citoskeleta i zbog toga je bila otežana vizualizacija komponenti postnatalne citodiferencijacije pljuvačnih žlezda. U ovoj studiji su, pomoću panela monoklonskih antitela (mAt), analizirane morfološke i imunofenotipske osobine CK polipeptida, u submandibularnoj pljuvač noj žlezdi (SPŽ) pacova tokom ontogeneze. Submandiblarne pljuvačne žlezde uzimane su od soja AO pacova, muškog pola, starosti 1, 30 i 60 dana. U ovom radu korišćena je imunohistohemijska metoda streptavidin-biotin peroksidaznog bojenja. Tkivni preseci fiksirani su u acetonu i inkubirani sa mAt specifičnim za CK polipeptide (CK7, CK 8, CK 18, CK bubrega, K8.12, K8.13) i epitel pacova (PT13D11). U ovoj imunohistohemijskoj analizi, ekspresija anti -CK antitela na SPŽ pacova, pokazuje fenotipsku heterogenost određenih komponenti ove žlezde tokom postnatalnog perioda. Epitelne ćelije acinusa su CK18+, dok epitelne ćelije kanalića imaju izražene CK definisane sa K8.12 i K8.13 mAt. Epitelne ćelije acinusa nisu bile obojene sa K8.12 i PT13D11 mAt. Umetnuti kanali su bili izrazito CK bubrega+ i PT13D11+. Mioepitelne ćelije acinusa su bile obojene sa K8.13 i PT13D11 mAt, dok su ove ćelije imale negativnu reakciju sa mAt specifičnim za obični epitel. Ova imunohistohemijska studija pokazuje heterogenost u ekspresiji CK polipeptida i može poslužiti za razumevanje porekla, razvoja i funkcije epitelnih ćelija SPŽ pacova.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis, Imunohistohemijska identifikacija citokeratina u submandibularnoj pljuvačnoj žlezdi pacova u toku ontogeneze",
volume = "59",
number = "1",
pages = "69-80",
doi = "10.2298/AVB0901069D"
}

Dožić, I., Todorović, T., Dožić, B.,& Čolić, M.. (2009). Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 69-80.
https://doi.org/10.2298/AVB0901069D

Dožić I, Todorović T, Dožić B, Čolić M. Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis. in Acta veterinaria. 2009;59(1):69-80.
doi:10.2298/AVB0901069D .

Dožić, Ivan, Todorović, Tatjana, Dožić, Branko, Čolić, Miodrag, "Immunohistochemical identification of cytokeratins in the rat submandibular salivary glands during ontogenesis" in Acta veterinaria, 59, no. 1 (2009):69-80,
https://doi.org/10.2298/AVB0901069D . .

TY  - JOUR
AU  - Stamenković, Dragoslav
AU  - Čairović, Aleksandra
AU  - Čolić, Miodrag
AU  - Rudolf, Rebeka
AU  - Radović, Katarina
AU  - Đorđević, Igor
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1478
AB  - Although highly noble Au alloys have been considered as biocompatible dental materials, little is known about whether recasting affects their biocompatibility. The aim of this work was to study the effect of repeated casting procedures on the microstructure and biocompatibilty of a noble Au dental alloy. The prepared samples of one- four- and eight times melted/cast Dentor S were used to study primary cutaneous irritation and sensibilization on experimental animals. The extract of the alloy prepared by conditioning the Dentor S samples in a cell culture medium was used to study the cytotoxicity on L929 cells. The elemental composition and surface changes of the alloy were examined by Energy Dispersive X-ray (EDX) analysis and optical microscopy, respectively. Our results showed that repeated casting decreased the biocompatibility of Dentor S, manifested as decreased metabolic and proliferative activities of L929 cells and that the effect correlated with the number of melting/casting procedures. However, neither sample of Dentor S caused irritation and sensibilization on experimental animals. EDX showed that recasting slightly increased the content of Au and Cu and slightly decreased the content of Pt, Pd and Zn. These changes correlated with the observations by optical microscopy. In conclusion, our results show that repeated casting of noble Au alloy changes its microstructure and decreases the biocompatibilty, suggesting that this procedure should be avoided in dental practice.
AB  - Visoko plemenite legure su biokompatibilni materijali, ali je malo poznato da li ponovljena livenja legura utiču na njihovu biokompatibilnost. Cilj ovog rada je bio da se utvrdi efekat ponovljenog topljenja i livenja na mikrostrukturu i biokompatibilnost visoko plemenitih dentalnih legura zlata. Pripremjena je serija uzoraka od jednom, četiri i osam puta topljene i livene Dentor S legure za ispitivanje primarne iritacije kože i senzibilizacije na eksperimentalnim životinjama, kao i in vitro ispitivanje citotoksičnosti na L929 ćelijama. Hemijski sastav i promena površine legure ispitivana je EDX analizom i optičkim mikroskopom. Naši rezultati su ukazali da ponavljana livenja smanjuju biokompatibilnost Dentor S legure, koja se manifestuje smanjenjem metabolitičke i proliferativne aktivnosti L929 ćelija i da je efekat u korelaciji s brojem topljenja i livenja. Ni jedan uzorak Dentor S legure ne uzrokuje iritaciju i senzibilizaciju eksperimentalnih životinja. EDX analiza dokazuje da ponovna livenja blago povećavaju procenat Au i Cu i blago smanjuju procenat Pt, Pd i Zn. Ove promene su u korelaciji sa zapažanjima na optičkom mikroskopu. Autori zaključuju da ponovljena livenja visoko plemenitih legura zlata menjaju mikrostrukturu i smanjuju biokompatibilnost i preporučuju da se ova procedura izbegava u svakodnevnoj praksi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effect of repeated casting on the biocompatibility of a dental gold alloy
T1  - Uticaj ponavljanog livenja na biokompatibilnost dentalnih legura zlata
VL  - 59
IS  - 5-6
SP  - 641
EP  - 652
DO  - 10.2298/AVB0906641S
ER  - 

@article{
author = "Stamenković, Dragoslav and Čairović, Aleksandra and Čolić, Miodrag and Rudolf, Rebeka and Radović, Katarina and Đorđević, Igor",
year = "2009",
abstract = "Although highly noble Au alloys have been considered as biocompatible dental materials, little is known about whether recasting affects their biocompatibility. The aim of this work was to study the effect of repeated casting procedures on the microstructure and biocompatibilty of a noble Au dental alloy. The prepared samples of one- four- and eight times melted/cast Dentor S were used to study primary cutaneous irritation and sensibilization on experimental animals. The extract of the alloy prepared by conditioning the Dentor S samples in a cell culture medium was used to study the cytotoxicity on L929 cells. The elemental composition and surface changes of the alloy were examined by Energy Dispersive X-ray (EDX) analysis and optical microscopy, respectively. Our results showed that repeated casting decreased the biocompatibility of Dentor S, manifested as decreased metabolic and proliferative activities of L929 cells and that the effect correlated with the number of melting/casting procedures. However, neither sample of Dentor S caused irritation and sensibilization on experimental animals. EDX showed that recasting slightly increased the content of Au and Cu and slightly decreased the content of Pt, Pd and Zn. These changes correlated with the observations by optical microscopy. In conclusion, our results show that repeated casting of noble Au alloy changes its microstructure and decreases the biocompatibilty, suggesting that this procedure should be avoided in dental practice., Visoko plemenite legure su biokompatibilni materijali, ali je malo poznato da li ponovljena livenja legura utiču na njihovu biokompatibilnost. Cilj ovog rada je bio da se utvrdi efekat ponovljenog topljenja i livenja na mikrostrukturu i biokompatibilnost visoko plemenitih dentalnih legura zlata. Pripremjena je serija uzoraka od jednom, četiri i osam puta topljene i livene Dentor S legure za ispitivanje primarne iritacije kože i senzibilizacije na eksperimentalnim životinjama, kao i in vitro ispitivanje citotoksičnosti na L929 ćelijama. Hemijski sastav i promena površine legure ispitivana je EDX analizom i optičkim mikroskopom. Naši rezultati su ukazali da ponavljana livenja smanjuju biokompatibilnost Dentor S legure, koja se manifestuje smanjenjem metabolitičke i proliferativne aktivnosti L929 ćelija i da je efekat u korelaciji s brojem topljenja i livenja. Ni jedan uzorak Dentor S legure ne uzrokuje iritaciju i senzibilizaciju eksperimentalnih životinja. EDX analiza dokazuje da ponovna livenja blago povećavaju procenat Au i Cu i blago smanjuju procenat Pt, Pd i Zn. Ove promene su u korelaciji sa zapažanjima na optičkom mikroskopu. Autori zaključuju da ponovljena livenja visoko plemenitih legura zlata menjaju mikrostrukturu i smanjuju biokompatibilnost i preporučuju da se ova procedura izbegava u svakodnevnoj praksi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effect of repeated casting on the biocompatibility of a dental gold alloy, Uticaj ponavljanog livenja na biokompatibilnost dentalnih legura zlata",
volume = "59",
number = "5-6",
pages = "641-652",
doi = "10.2298/AVB0906641S"
}

Stamenković, D., Čairović, A., Čolić, M., Rudolf, R., Radović, K.,& Đorđević, I.. (2009). The effect of repeated casting on the biocompatibility of a dental gold alloy. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 641-652.
https://doi.org/10.2298/AVB0906641S

Stamenković D, Čairović A, Čolić M, Rudolf R, Radović K, Đorđević I. The effect of repeated casting on the biocompatibility of a dental gold alloy. in Acta veterinaria. 2009;59(5-6):641-652.
doi:10.2298/AVB0906641S .

Stamenković, Dragoslav, Čairović, Aleksandra, Čolić, Miodrag, Rudolf, Rebeka, Radović, Katarina, Đorđević, Igor, "The effect of repeated casting on the biocompatibility of a dental gold alloy" in Acta veterinaria, 59, no. 5-6 (2009):641-652,
https://doi.org/10.2298/AVB0906641S . .

TY  - JOUR
AU  - Todorović, Vera
AU  - Marković, Dejan
AU  - Milošević-Jovčić, Nadežda
AU  - Petakov, Marijana
AU  - Balint, Bela
AU  - Čolić, Miodrag
AU  - Milenković, Ana
AU  - Čolak, Ivana
AU  - Jokanović, Vukoman
AU  - Nikolić, Nebojša
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1412
AB  - To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages.
AB  - Iz zubne pulpe su do danas izolovane tri populacije matičnih ćelija koje su označene kao matične ćelije zubne pulpe (engl. Dental Pulp Stem Cells, DPSC), matične ćelije iz eksfoliranih mlečnih zuba (engl. Stem Cells From Human Exfoliated Decidual Teeth, SHED) i nezrele matične ćelije zubne pulpe (engl. Immature Dental Pulp Stem Cells, IDPC). Sve matične ćelije zubne pulpe su ektomezenhimalnog porekla i lokalizovane su u perivaskularnoj niši. One se lako i efikasno izoluju, visoko su proliferativne, klonogene, multipotentne, ispoljavaju visok stepen plasticiteta i i slične su mezenhimalnim matičnim ćelijama koštane srži (BMSC). U njima je pokazana visoka ekspresija gena alkalne fosfataze, proteina 1 matriksa dentina i dentin-sijalofosfoproteina. Takođe, istaknuta je važnost u ovim ćelijama ekspresije više gena koji kodiraju sintezu komponenti ekstracelularnog matriksa, molekula ćelijske adhezije, faktora rasta, transkripcionih faktora, gena prenosa ćelijskih signala, ćelijske komunikacije i metabolizma. U uslovima in vitro ili in vivo ove ćelije mogu da se diferenciraju, s određenim međusobnim razlikama, u pravcu odontoblasta, hondrocita, osteoblasta, adipocita, neurona/glije, glatkih i skeletnih mišićnih ćelija, endotelnih ćelija i melanocita. U uslovima in vivo, nakon implantacije, pokazuju različit potencijal za formiranje dentina, ali i koštanog, masnog i nervnog tkiva. Generalno se smatra da DPSC imaju anti-inflamatorno dejstvo i ispoljavaju imunom-odulatorni efekat. Takođe, dovode do imunološke tolerancije ukoliko se implantiraju u alogena tkiva. Sposobnost inhibicije proliferacije T limfocita ukazuje na njihovo imunosupresivno dejstvo. Matične ćelije zubne pulpe otvorile su nove perspektive u terapijskoj primeni ovih ćelija ne samo u regeneraciji dentina, tkiva periodoncijuma i koštano-zglobnog tkiva kraniofacijalne regije, već i u lečenju neurotraume, autoimunskih oboljenja, infarkta miokarda, mišićne distrofije i oštećenja vezivnog tkiva.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Dental pulp stem cells: Potential significance in regenerative medicine
T1  - Matične ćelije zubne pulpe i njihov potencijalni značaj u regenerativnoj medicini
VL  - 55
IS  - 3
SP  - 170
EP  - 179
DO  - 10.2298/SGS0803170T
ER  - 

@article{
author = "Todorović, Vera and Marković, Dejan and Milošević-Jovčić, Nadežda and Petakov, Marijana and Balint, Bela and Čolić, Miodrag and Milenković, Ana and Čolak, Ivana and Jokanović, Vukoman and Nikolić, Nebojša",
year = "2008",
abstract = "To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages., Iz zubne pulpe su do danas izolovane tri populacije matičnih ćelija koje su označene kao matične ćelije zubne pulpe (engl. Dental Pulp Stem Cells, DPSC), matične ćelije iz eksfoliranih mlečnih zuba (engl. Stem Cells From Human Exfoliated Decidual Teeth, SHED) i nezrele matične ćelije zubne pulpe (engl. Immature Dental Pulp Stem Cells, IDPC). Sve matične ćelije zubne pulpe su ektomezenhimalnog porekla i lokalizovane su u perivaskularnoj niši. One se lako i efikasno izoluju, visoko su proliferativne, klonogene, multipotentne, ispoljavaju visok stepen plasticiteta i i slične su mezenhimalnim matičnim ćelijama koštane srži (BMSC). U njima je pokazana visoka ekspresija gena alkalne fosfataze, proteina 1 matriksa dentina i dentin-sijalofosfoproteina. Takođe, istaknuta je važnost u ovim ćelijama ekspresije više gena koji kodiraju sintezu komponenti ekstracelularnog matriksa, molekula ćelijske adhezije, faktora rasta, transkripcionih faktora, gena prenosa ćelijskih signala, ćelijske komunikacije i metabolizma. U uslovima in vitro ili in vivo ove ćelije mogu da se diferenciraju, s određenim međusobnim razlikama, u pravcu odontoblasta, hondrocita, osteoblasta, adipocita, neurona/glije, glatkih i skeletnih mišićnih ćelija, endotelnih ćelija i melanocita. U uslovima in vivo, nakon implantacije, pokazuju različit potencijal za formiranje dentina, ali i koštanog, masnog i nervnog tkiva. Generalno se smatra da DPSC imaju anti-inflamatorno dejstvo i ispoljavaju imunom-odulatorni efekat. Takođe, dovode do imunološke tolerancije ukoliko se implantiraju u alogena tkiva. Sposobnost inhibicije proliferacije T limfocita ukazuje na njihovo imunosupresivno dejstvo. Matične ćelije zubne pulpe otvorile su nove perspektive u terapijskoj primeni ovih ćelija ne samo u regeneraciji dentina, tkiva periodoncijuma i koštano-zglobnog tkiva kraniofacijalne regije, već i u lečenju neurotraume, autoimunskih oboljenja, infarkta miokarda, mišićne distrofije i oštećenja vezivnog tkiva.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Dental pulp stem cells: Potential significance in regenerative medicine, Matične ćelije zubne pulpe i njihov potencijalni značaj u regenerativnoj medicini",
volume = "55",
number = "3",
pages = "170-179",
doi = "10.2298/SGS0803170T"
}

Todorović, V., Marković, D., Milošević-Jovčić, N., Petakov, M., Balint, B., Čolić, M., Milenković, A., Čolak, I., Jokanović, V.,& Nikolić, N.. (2008). Dental pulp stem cells: Potential significance in regenerative medicine. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 55(3), 170-179.
https://doi.org/10.2298/SGS0803170T

Todorović V, Marković D, Milošević-Jovčić N, Petakov M, Balint B, Čolić M, Milenković A, Čolak I, Jokanović V, Nikolić N. Dental pulp stem cells: Potential significance in regenerative medicine. in Stomatološki glasnik Srbije. 2008;55(3):170-179.
doi:10.2298/SGS0803170T .

Todorović, Vera, Marković, Dejan, Milošević-Jovčić, Nadežda, Petakov, Marijana, Balint, Bela, Čolić, Miodrag, Milenković, Ana, Čolak, Ivana, Jokanović, Vukoman, Nikolić, Nebojša, "Dental pulp stem cells: Potential significance in regenerative medicine" in Stomatološki glasnik Srbije, 55, no. 3 (2008):170-179,
https://doi.org/10.2298/SGS0803170T . .

TY  - JOUR
AU  - Dožić, Ivan
AU  - Čolić, Miodrag
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1438
AB  - Aim: The aim of this study was to investigate the expression of monoclonal antibody (mAbs) in the myoepithelial cells of the rat submandibular salivary glands (SSG) during postnatal development. Material and method: SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with mAbs which specifically reacts with cytokeratins (CK) polypeptides ( CK7, CK8, CK18, CK kidney, K8.12, K8.13), rats epithelial (PT13D11), rats collagen (RMC-23), laminin (α6β1) and rats sialoadhesin (ED3). Results: Immunohistochemical analysis of the myoepithelial cells showed phenotypic heterogeneity during the postnatal development. Myoepithelial cells of acini were stained by K8.13, PT13D11 and ED3 mAbs, whereas these cells were not stained by mAbs CK7, CK8, CK18, CK-kidney, RMC-23 and α6β1. In adult rats, myoepithelial cells of excretory duct were stained by K8.12 mAb. Conclusion: The obtained results show phenotypic heterogeneity of myoepithelial cells and might also be useful to further understanding of myoepithelial cells development and functions.
AB  - Cilj ovog rada je bio da se ispita ekspresija monoklonskih antitela (mAt) u mioepitelnim ćelijama podvilične pljuvačne žlezde (PPŽ) pacova tokom postnatalnog razvoja. Materijal i metode: Istraživanja su rađena na pacovima soja AO, muškog pola, starosti 1, 30, 60 dana. Korišćena je imunohistohemijska metoda streptavidin-biotin peroksidazno bojenje. Kriostatski preseci su fiksirani u acetonu, inkubirani su sa mAt specifičnim za polipeptide citokeratina (CK) (CK7, CK8, CK18, CK bubrega, K8.12, K8.13), epitel pacova (PT13D11), kolagen pacova (RMC-23), laminin (α6β1) i sijaloadhezin pacova (ED3). Rezultati: Dobijeni rezlutati ove imunhistohemijske analize su pokazali fenotipsku heterogenost mioepitelnih ćelija tokom postnatalnog razvoja. Mioepitelne ćelije oko acinusa bile su reaktivne sa mAt K8.13, PT13D11 i ED3, dok su pokazale negativnu reakciju sa CK7, CK8, CK18, CK-bubrega, RMC-23 i α6β1 mAt. Kod odraslih životinja (60 dana), mioepitelne ćelije oko ekskretornih kanala su bile obojene sa K8.12 mAt. Zaključak: Ovi rezultati pokazuju fenotipsku heterogenost mioepitelnih ćelija i mogu biti korisni za razumevanje razvoja i funkcije ovih ćelija.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Immunohistological characterization of myoepithelial cells using monoclonal antibodies
T1  - Imunohistološke karakteristike mioepitelnih ćelija pomoću monoklonskih antitela
VL  - 55
IS  - 1
SP  - 7
EP  - 14
DO  - 10.2298/SGS0801007D
ER  - 

@article{
author = "Dožić, Ivan and Čolić, Miodrag",
year = "2008",
abstract = "Aim: The aim of this study was to investigate the expression of monoclonal antibody (mAbs) in the myoepithelial cells of the rat submandibular salivary glands (SSG) during postnatal development. Material and method: SSG were obtained from 1, 30 and 60 days old, male, AO rats. Streptavidin-biotin immunoperoxidase method was used for immunohistochemistry. Cryostat sections were fixed in acetone and incubated with mAbs which specifically reacts with cytokeratins (CK) polypeptides ( CK7, CK8, CK18, CK kidney, K8.12, K8.13), rats epithelial (PT13D11), rats collagen (RMC-23), laminin (α6β1) and rats sialoadhesin (ED3). Results: Immunohistochemical analysis of the myoepithelial cells showed phenotypic heterogeneity during the postnatal development. Myoepithelial cells of acini were stained by K8.13, PT13D11 and ED3 mAbs, whereas these cells were not stained by mAbs CK7, CK8, CK18, CK-kidney, RMC-23 and α6β1. In adult rats, myoepithelial cells of excretory duct were stained by K8.12 mAb. Conclusion: The obtained results show phenotypic heterogeneity of myoepithelial cells and might also be useful to further understanding of myoepithelial cells development and functions., Cilj ovog rada je bio da se ispita ekspresija monoklonskih antitela (mAt) u mioepitelnim ćelijama podvilične pljuvačne žlezde (PPŽ) pacova tokom postnatalnog razvoja. Materijal i metode: Istraživanja su rađena na pacovima soja AO, muškog pola, starosti 1, 30, 60 dana. Korišćena je imunohistohemijska metoda streptavidin-biotin peroksidazno bojenje. Kriostatski preseci su fiksirani u acetonu, inkubirani su sa mAt specifičnim za polipeptide citokeratina (CK) (CK7, CK8, CK18, CK bubrega, K8.12, K8.13), epitel pacova (PT13D11), kolagen pacova (RMC-23), laminin (α6β1) i sijaloadhezin pacova (ED3). Rezultati: Dobijeni rezlutati ove imunhistohemijske analize su pokazali fenotipsku heterogenost mioepitelnih ćelija tokom postnatalnog razvoja. Mioepitelne ćelije oko acinusa bile su reaktivne sa mAt K8.13, PT13D11 i ED3, dok su pokazale negativnu reakciju sa CK7, CK8, CK18, CK-bubrega, RMC-23 i α6β1 mAt. Kod odraslih životinja (60 dana), mioepitelne ćelije oko ekskretornih kanala su bile obojene sa K8.12 mAt. Zaključak: Ovi rezultati pokazuju fenotipsku heterogenost mioepitelnih ćelija i mogu biti korisni za razumevanje razvoja i funkcije ovih ćelija.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Immunohistological characterization of myoepithelial cells using monoclonal antibodies, Imunohistološke karakteristike mioepitelnih ćelija pomoću monoklonskih antitela",
volume = "55",
number = "1",
pages = "7-14",
doi = "10.2298/SGS0801007D"
}

Dožić, I.,& Čolić, M.. (2008). Immunohistological characterization of myoepithelial cells using monoclonal antibodies. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 55(1), 7-14.
https://doi.org/10.2298/SGS0801007D

Dožić I, Čolić M. Immunohistological characterization of myoepithelial cells using monoclonal antibodies. in Stomatološki glasnik Srbije. 2008;55(1):7-14.
doi:10.2298/SGS0801007D .

Dožić, Ivan, Čolić, Miodrag, "Immunohistological characterization of myoepithelial cells using monoclonal antibodies" in Stomatološki glasnik Srbije, 55, no. 1 (2008):7-14,
https://doi.org/10.2298/SGS0801007D . .

TY  - JOUR
AU  - Dožić, Ivan
AU  - Čolić, Miodrag
PY  - 2003
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1183
AB  - The aim of this study was to investigate the expression of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during postnatal development (1st, 30th,and 60th day) by using various monoclonal antibodies (mAbs) RMC-23 (specific for collagen),a6ß1 (specific for laminin) and ED3 (specific for sialoadhezin). These components of extracellular matrix were detected. RMC-23 mAb showed strong positivity to the basal membranes of the ductal system (intercalated, striated and excretory ducts) and of intersticium. Increased expression in the basal membrane of acini during development of glands was noted. Similar immunoreactivity was shown byßmAb but the intersticium showed a negative reaction to 1a6 this antibody. Positive reactivity of ß1a6 mAb of epithelial ductal cells, particularly of the neonatal animals, was found. In contrast to ß 1a6 and RMC23 mAbs, ED3 mAb was increasingly expressed in the myoepithelial cells during ontogenesis. Our findings regarding the immunoreactivity of collagens and laminins are in accordance with the findings of other autors. The very interesting finding of sialoadhesin in myoepithelial cells of the rat submandibular salivary gland, which is not described in literature and needs further investigation. Our results suggest that adhesion molecules and extracellular matrix proteins have an important biochemical role during postnatal development of the submandibular salivary gland.
AB  - Cilj ovog rada je bio da se ispita ekspresija kolagena, laminina i sijaloadhezina u podviličnoj pljuvačnoj žlezdi (PPŽ) pacova tokom postnatalnog razvoja (1, 30, 60 dana). Imunohistohemijski streptavidin-biotin peroksidaznom bojenjem, upotrebom različitih monoklonskih antitela RMC-23 (specifično za kolagen), aß1(specifično za laminin) i ED3 (specifično za sijaloadhezin) detektovane su ove komponente ekstraćelijskog matriksa. RMC-23 mAt se snažno vezuje za bazalne membrane izvodnih kanala (umetnuti, prugasti i ekskretorni kanali) i intersticijum. Ekspresija u bazalnim membranama acinusa se povećava u toku razvoja žlezde. Sličnu imunoreaktivnost ima aß1 mAt, ali je intersticijum pokazivao negativnu reakciju. Epitelne ćelije izvodnih kanala posebno neonatalnih životinja bile suaß+. ED3 mAt je za razliku odaßi RMC-23 mAt detektovano i u mioepitelnim ćelijama čija se reaktivnost povećava tokom ontogeneze. Veoma je interesantan nalaz sijaloadhezina u mioepitelnim ćelijama PPŽ pacova koji nije opisan u literaturi, što zahteva dalja ispitivanja. Ovi rezultati pokazuju da adhezioni molekuli i proteini ekstraćelijskog matriksa imaju značajnu biohemijsku ulogu u toku postnatalnog razvoja podvilične pljuvačne žlezde.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis
T1  - Imunohistološke karaktersitike kolagena, laminina i sijaloadhezina u podviličnoj pljuvačnoj žlezdi pacova tokom ontogeneze
VL  - 50
IS  - 4
SP  - 179
EP  - 183
DO  - 10.2298/SGS0304179D
ER  - 

@article{
author = "Dožić, Ivan and Čolić, Miodrag",
year = "2003",
abstract = "The aim of this study was to investigate the expression of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during postnatal development (1st, 30th,and 60th day) by using various monoclonal antibodies (mAbs) RMC-23 (specific for collagen),a6ß1 (specific for laminin) and ED3 (specific for sialoadhezin). These components of extracellular matrix were detected. RMC-23 mAb showed strong positivity to the basal membranes of the ductal system (intercalated, striated and excretory ducts) and of intersticium. Increased expression in the basal membrane of acini during development of glands was noted. Similar immunoreactivity was shown byßmAb but the intersticium showed a negative reaction to 1a6 this antibody. Positive reactivity of ß1a6 mAb of epithelial ductal cells, particularly of the neonatal animals, was found. In contrast to ß 1a6 and RMC23 mAbs, ED3 mAb was increasingly expressed in the myoepithelial cells during ontogenesis. Our findings regarding the immunoreactivity of collagens and laminins are in accordance with the findings of other autors. The very interesting finding of sialoadhesin in myoepithelial cells of the rat submandibular salivary gland, which is not described in literature and needs further investigation. Our results suggest that adhesion molecules and extracellular matrix proteins have an important biochemical role during postnatal development of the submandibular salivary gland., Cilj ovog rada je bio da se ispita ekspresija kolagena, laminina i sijaloadhezina u podviličnoj pljuvačnoj žlezdi (PPŽ) pacova tokom postnatalnog razvoja (1, 30, 60 dana). Imunohistohemijski streptavidin-biotin peroksidaznom bojenjem, upotrebom različitih monoklonskih antitela RMC-23 (specifično za kolagen), aß1(specifično za laminin) i ED3 (specifično za sijaloadhezin) detektovane su ove komponente ekstraćelijskog matriksa. RMC-23 mAt se snažno vezuje za bazalne membrane izvodnih kanala (umetnuti, prugasti i ekskretorni kanali) i intersticijum. Ekspresija u bazalnim membranama acinusa se povećava u toku razvoja žlezde. Sličnu imunoreaktivnost ima aß1 mAt, ali je intersticijum pokazivao negativnu reakciju. Epitelne ćelije izvodnih kanala posebno neonatalnih životinja bile suaß+. ED3 mAt je za razliku odaßi RMC-23 mAt detektovano i u mioepitelnim ćelijama čija se reaktivnost povećava tokom ontogeneze. Veoma je interesantan nalaz sijaloadhezina u mioepitelnim ćelijama PPŽ pacova koji nije opisan u literaturi, što zahteva dalja ispitivanja. Ovi rezultati pokazuju da adhezioni molekuli i proteini ekstraćelijskog matriksa imaju značajnu biohemijsku ulogu u toku postnatalnog razvoja podvilične pljuvačne žlezde.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis, Imunohistološke karaktersitike kolagena, laminina i sijaloadhezina u podviličnoj pljuvačnoj žlezdi pacova tokom ontogeneze",
volume = "50",
number = "4",
pages = "179-183",
doi = "10.2298/SGS0304179D"
}

Dožić, I.,& Čolić, M.. (2003). Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 50(4), 179-183.
https://doi.org/10.2298/SGS0304179D

Dožić I, Čolić M. Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis. in Stomatološki glasnik Srbije. 2003;50(4):179-183.
doi:10.2298/SGS0304179D .

Dožić, Ivan, Čolić, Miodrag, "Immunohistological characteristics of collagen, laminin and sialoadhesin in the rat submandibular salivary gland during ontogenesis" in Stomatološki glasnik Srbije, 50, no. 4 (2003):179-183,
https://doi.org/10.2298/SGS0304179D . .