TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Gomez-Moreno, Gerardo
AU  - Aguilar-Salvatierra, Antonio
AU  - Marković, Aleksa
AU  - Eduardo Mate-Sanchez, Jose
AU  - Calvo Guirado, Jose Luis
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2125
AB  - ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.
PB  - Wiley, Hoboken
T2  - Clinical Oral Implants Research
T1  - Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study
VL  - 27
IS  - 11
SP  - e144
EP  - e153
DO  - 10.1111/clr.12585
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Gomez-Moreno, Gerardo and Aguilar-Salvatierra, Antonio and Marković, Aleksa and Eduardo Mate-Sanchez, Jose and Calvo Guirado, Jose Luis",
year = "2016",
abstract = "ObjectivesTo measure the lateral surface area of microgrooved zirconia implants, to evaluate the cell geometry and cell density of human fetal osteoblasts seeded on zirconia microgrooved implants, to describe the surface roughness and chemistry, and to evaluate the activity of human fetal osteoblasts seeded on zirconia microgrooved disks. Materials and methodsThis experimental in vitro study used 62 zirconia implants and 130 zirconia disks. Two experimental groups were created for the implants: 31 non-microgrooved implants (Control) and 31 microgrooved implants (Test); two experimental groups were created for the disks: 65 non-microgrooved disks (Control) and 65 microgrooved disks (Test). The following evaluations of the implants were made: lateral surface area (LSA), cell morphology, and density of human fetal osteoblasts seeded on implant surfaces. On the disks, surface parameters (roughness and chemistry) and cell activity (alkaline phosphatase - ALP and alizarin red - ALZ) were evaluated at 7 and 15days. ResultsLSA was lower for control implants (62.8mm) compared with test implants (128.74mm) (P lt 0.05). Cell bodies on control surfaces were flattened and disorganized, while in the test group, they were aligned inside the microgrooves. Control group cells showed few lamellipodia, which were attached mainly inside topographical accidents (surface cracks, valleys, and pits). Test group implants presented cells rich in lamellipodia prolongations, attached to the inner walls or to the borders of the microgrooves and in the flat areas between the microgrooves. Cell density was higher in the test group compared with controls (P lt 0.05) Surface roughness and oxygen content increased in test disks samples compared with controls (P lt 0.05). Carbon and aluminum were reduced in disks test samples compared with controls (P lt 0.05), and ALP and ALZ levels were significantly increased on test surfaces (P lt 0.05) at both study times. ConclusionsWithin the limitations of this experimental study, it may be concluded that (i) Roughness is increased and chemical composition enhanced on the surface of zirconia implants with microgrooves. (ii) The LSA of microgrooved zirconia implants is greater and provides more available surface compared with implants of the same dimensions without microgrooves. (iii) Microgrooves on zirconia implants modify the morphology and guide the size and alignment of human fetal osteoblasts. (iv) Zirconia surfaces with microgrooves of 30m width and 70m separation between grooves enhance ALP and ALZ expression by human fetal osteoblasts.",
publisher = "Wiley, Hoboken",
journal = "Clinical Oral Implants Research",
title = "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study",
volume = "27",
number = "11",
pages = "e144-e153",
doi = "10.1111/clr.12585"
}

Delgado-Ruiz, R., Gomez-Moreno, G., Aguilar-Salvatierra, A., Marković, A., Eduardo Mate-Sanchez, J.,& Calvo Guirado, J. L.. (2016). Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research
Wiley, Hoboken., 27(11), e144-e153.
https://doi.org/10.1111/clr.12585

Delgado-Ruiz R, Gomez-Moreno G, Aguilar-Salvatierra A, Marković A, Eduardo Mate-Sanchez J, Calvo Guirado JL. Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study. in Clinical Oral Implants Research. 2016;27(11):e144-e153.
doi:10.1111/clr.12585 .

Delgado-Ruiz, Rafael, Gomez-Moreno, Gerardo, Aguilar-Salvatierra, Antonio, Marković, Aleksa, Eduardo Mate-Sanchez, Jose, Calvo Guirado, Jose Luis, "Human fetal osteoblast behavior on zirconia dental implants and zirconia disks with microstructured surfaces. An experimental in vitro study" in Clinical Oral Implants Research, 27, no. 11 (2016):e144-e153,
https://doi.org/10.1111/clr.12585 . .

TY  - JOUR
AU  - Gomez-Moreno, Gerardo
AU  - Aguilar-Salvatierra, Antonio
AU  - Fernandez-Cejas, Esther
AU  - Arcesio Delgado-Ruiz, Rafael
AU  - Marković, Aleksa
AU  - Calvo Guirado, Jose Luis
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2088
AB  - Objectives: The aim of this study was to evaluate the incidence of bleeding complications after dental implant placement in patients in treatment by the anticoagulant oral rivaroxaban without interrupting its administration or modifying dosage. Materials and methods: About 57 patients were divided into two groups: 18 had been in treatment by rivaroxaban for over 6 month before implant surgery and a control group consisted of 39 healthy subjects. All subjects received dental implants in different positions, without interrupting or modifying rivaroxaban dosage. Patients were treated in an outpatient setting. Non-absorbable sutures were used, and all patients were given gauze impregnated with tranexamic acid 5%, to bite on for 30 60 min. Results: One rivaroxaban patient presented moderate bleeding the day after surgery, and two control patients presented moderate bleeding the day after and on the second day. Bleeding was managed with gauzes impregnated with tranexamic acid. No statistically significant differences (P 0.688) were found in relation to bleeding episodes between the groups, with a relative risk 0.919 based on the pooled groups and 95% confidence interval of 0.078 10.844. Conclusions: Dental implant surgery in patients taking the anticoagulant oral rivaroxaban can be performed safely in outpatients departments applying local hemostatic measures without the need to modify or interrupt anticoagulant medication.
PB  - Wiley, Hoboken
T2  - Clinical Oral Implants Research
T1  - Dental implant surgery in patients in treatment with the anticoagulant oral rivaroxaban
VL  - 27
IS  - 6
SP  - 730
EP  - 733
DO  - 10.1111/clr.12653
ER  - 

@article{
author = "Gomez-Moreno, Gerardo and Aguilar-Salvatierra, Antonio and Fernandez-Cejas, Esther and Arcesio Delgado-Ruiz, Rafael and Marković, Aleksa and Calvo Guirado, Jose Luis",
year = "2016",
abstract = "Objectives: The aim of this study was to evaluate the incidence of bleeding complications after dental implant placement in patients in treatment by the anticoagulant oral rivaroxaban without interrupting its administration or modifying dosage. Materials and methods: About 57 patients were divided into two groups: 18 had been in treatment by rivaroxaban for over 6 month before implant surgery and a control group consisted of 39 healthy subjects. All subjects received dental implants in different positions, without interrupting or modifying rivaroxaban dosage. Patients were treated in an outpatient setting. Non-absorbable sutures were used, and all patients were given gauze impregnated with tranexamic acid 5%, to bite on for 30 60 min. Results: One rivaroxaban patient presented moderate bleeding the day after surgery, and two control patients presented moderate bleeding the day after and on the second day. Bleeding was managed with gauzes impregnated with tranexamic acid. No statistically significant differences (P 0.688) were found in relation to bleeding episodes between the groups, with a relative risk 0.919 based on the pooled groups and 95% confidence interval of 0.078 10.844. Conclusions: Dental implant surgery in patients taking the anticoagulant oral rivaroxaban can be performed safely in outpatients departments applying local hemostatic measures without the need to modify or interrupt anticoagulant medication.",
publisher = "Wiley, Hoboken",
journal = "Clinical Oral Implants Research",
title = "Dental implant surgery in patients in treatment with the anticoagulant oral rivaroxaban",
volume = "27",
number = "6",
pages = "730-733",
doi = "10.1111/clr.12653"
}

Gomez-Moreno, G., Aguilar-Salvatierra, A., Fernandez-Cejas, E., Arcesio Delgado-Ruiz, R., Marković, A.,& Calvo Guirado, J. L.. (2016). Dental implant surgery in patients in treatment with the anticoagulant oral rivaroxaban. in Clinical Oral Implants Research
Wiley, Hoboken., 27(6), 730-733.
https://doi.org/10.1111/clr.12653

Gomez-Moreno G, Aguilar-Salvatierra A, Fernandez-Cejas E, Arcesio Delgado-Ruiz R, Marković A, Calvo Guirado JL. Dental implant surgery in patients in treatment with the anticoagulant oral rivaroxaban. in Clinical Oral Implants Research. 2016;27(6):730-733.
doi:10.1111/clr.12653 .

Gomez-Moreno, Gerardo, Aguilar-Salvatierra, Antonio, Fernandez-Cejas, Esther, Arcesio Delgado-Ruiz, Rafael, Marković, Aleksa, Calvo Guirado, Jose Luis, "Dental implant surgery in patients in treatment with the anticoagulant oral rivaroxaban" in Clinical Oral Implants Research, 27, no. 6 (2016):730-733,
https://doi.org/10.1111/clr.12653 . .

TY  - JOUR
AU  - Delgado-Ruiz, Rafael
AU  - Calvo Guirado, Jose Luis
AU  - Abboud, Marcus
AU  - Piedad Ramirez-Fernandez, Maria
AU  - Eduardo Mate-Sanchez, Jose
AU  - Negri, Bruno
AU  - Gomez-Moreno, Gerardo
AU  - Marković, Aleksa
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1970
AB  - PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light
VL  - 17
IS  - 4
SP  - 667
EP  - 680
DO  - 10.1111/cid.12161
ER  - 

@article{
author = "Delgado-Ruiz, Rafael and Calvo Guirado, Jose Luis and Abboud, Marcus and Piedad Ramirez-Fernandez, Maria and Eduardo Mate-Sanchez, Jose and Negri, Bruno and Gomez-Moreno, Gerardo and Marković, Aleksa",
year = "2015",
abstract = "PurposeTo describe contact, thickness, density, and orientation of connective tissue fibers around healing abutments of different geometries by means of a new method using coordinates. Materials and MethodsFollowing the bilateral extraction of mandibular premolars (P2, P3, and P4) from six fox hound dogs and a 2-month healing period, 36 titanium implants were inserted, onto which two groups of healing abutments of different geometry were screwed: Group A (concave abutments) and Group B (wider healing abutment). After 3 months the animals were sacrificed and samples extracted containing each implant and surrounding soft and hard tissues. Histological analysis was performed without decalcifying the samples by means of circularly polarized light under optical microscope and a system of vertical and horizontal coordinates across all the connective tissue in an area delimited by the implant/abutment, epithelium, and bone tissue. ResultsIn no case had the connective tissue formed a connection to the healing abutment/implant in the internal zone; a space of 3510m separated the connective tissue fibers from the healing abutment surface. The total thickness of connective tissue in the horizontal direction was significantly greater in the medial zone in Group B than in Group A (p lt .05). The orientation of the fibers varied according to the coordinate area so that internal coordinates showed a higher percentage of parallel fibers in Group A (p lt .05) and a higher percentage of oblique fibers in Group B (p lt .05); medial coordinates showed more oblique fibers (p lt .05); and the area of external coordinates showed the highest percentage of perpendicular fibers (p lt .05). The fiber density was higher in the basal and medial areas (p lt .05). ConclusionsAbutment geometry influences the orientation of collagen fibers; therefore, an abutment with a profile wider than the implant platform favors oblique and perpendicular orientation of collagen fibers and greater connective tissue thickness.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light",
volume = "17",
number = "4",
pages = "667-680",
doi = "10.1111/cid.12161"
}

Delgado-Ruiz, R., Calvo Guirado, J. L., Abboud, M., Piedad Ramirez-Fernandez, M., Eduardo Mate-Sanchez, J., Negri, B., Gomez-Moreno, G.,& Marković, A.. (2015). Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 17(4), 667-680.
https://doi.org/10.1111/cid.12161

Delgado-Ruiz R, Calvo Guirado JL, Abboud M, Piedad Ramirez-Fernandez M, Eduardo Mate-Sanchez J, Negri B, Gomez-Moreno G, Marković A. Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light. in Clinical Implant Dentistry & Related Research. 2015;17(4):667-680.
doi:10.1111/cid.12161 .

Delgado-Ruiz, Rafael, Calvo Guirado, Jose Luis, Abboud, Marcus, Piedad Ramirez-Fernandez, Maria, Eduardo Mate-Sanchez, Jose, Negri, Bruno, Gomez-Moreno, Gerardo, Marković, Aleksa, "Connective Tissue Characteristics around Healing Abutments of Different Geometries: New Methodological Technique under Circularly Polarized Light" in Clinical Implant Dentistry & Related Research, 17, no. 4 (2015):667-680,
https://doi.org/10.1111/cid.12161 . .

TY  - JOUR
AU  - Marković, Aleksa
AU  - Luis Calvo-Guirado, Jose
AU  - Lazić, Zoran
AU  - Gomez-Moreno, Gerardo
AU  - Ćalasan, Dejan
AU  - Guardia, Javier
AU  - Čolić, Snježana
AU  - Aguilar-Salvatierra, Antonio
AU  - Gačić, Bojan
AU  - Delgado-Ruiz, Rafael
AU  - Janjić, Bojan
AU  - Mišić, Tijana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1816
AB  - Purpose: The aim of this study was to investigate the relationship between surgical techniques and implant macro-design (self-tapping/non-self-tapping) for the optimization of implant stability in the low-density bone present in the posterior maxilla using resonance frequency analysis (RFA). Materials and Methods: A total of 102 implants were studied. Fifty-six self-tapping BlueSkyBredent (R) (Bredent GmbH &, Senden, Germany) and 56 non-self-tapping Standard Plus Straumann (R) (Institut Straumann AG (R), Waldenburg, Switzerland) were placed in the posterior segment of the maxilla. Implants of both types were placed in sites prepared with either lateral bone-condensing or with bone-drilling techniques. Implant stability measurements were performed using RFA immediately after implant placement and weekly during a 12-week follow-up period. Results: Both types of implants placed after bone condensing achieved significantly higher stability immediately after surgery, as well as during the entire 12-week observation period compared with those placed following bone drilling. After bone condensation, there were no significant differences in primary stability or in implant stability after the first week between both implant types. From 2 to 12 postoperative weeks, significantly higher stability was shown by self-tapping implants. After bone drilling, self-tapping implants achieved significantly higher stability than non-self-tapping implants during the entire follow-up period. Conclusions: The outcomes of the present study indicate that bone drilling is not an effective technique for improving implant stability and, following this technique, the use of self-tapping implants is highly recommended. Implant stability optimization in the soft bone can be achieved by lateral bone-condensing technique, regardless of implant macro-design.
PB  - Wiley, Hoboken
T2  - Clinical Implant Dentistry & Related Research
T1  - Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study
VL  - 15
IS  - 3
SP  - 341
EP  - 349
DO  - 10.1111/j.1708-8208.2011.00415.x
ER  - 

@article{
author = "Marković, Aleksa and Luis Calvo-Guirado, Jose and Lazić, Zoran and Gomez-Moreno, Gerardo and Ćalasan, Dejan and Guardia, Javier and Čolić, Snježana and Aguilar-Salvatierra, Antonio and Gačić, Bojan and Delgado-Ruiz, Rafael and Janjić, Bojan and Mišić, Tijana",
year = "2013",
abstract = "Purpose: The aim of this study was to investigate the relationship between surgical techniques and implant macro-design (self-tapping/non-self-tapping) for the optimization of implant stability in the low-density bone present in the posterior maxilla using resonance frequency analysis (RFA). Materials and Methods: A total of 102 implants were studied. Fifty-six self-tapping BlueSkyBredent (R) (Bredent GmbH &, Senden, Germany) and 56 non-self-tapping Standard Plus Straumann (R) (Institut Straumann AG (R), Waldenburg, Switzerland) were placed in the posterior segment of the maxilla. Implants of both types were placed in sites prepared with either lateral bone-condensing or with bone-drilling techniques. Implant stability measurements were performed using RFA immediately after implant placement and weekly during a 12-week follow-up period. Results: Both types of implants placed after bone condensing achieved significantly higher stability immediately after surgery, as well as during the entire 12-week observation period compared with those placed following bone drilling. After bone condensation, there were no significant differences in primary stability or in implant stability after the first week between both implant types. From 2 to 12 postoperative weeks, significantly higher stability was shown by self-tapping implants. After bone drilling, self-tapping implants achieved significantly higher stability than non-self-tapping implants during the entire follow-up period. Conclusions: The outcomes of the present study indicate that bone drilling is not an effective technique for improving implant stability and, following this technique, the use of self-tapping implants is highly recommended. Implant stability optimization in the soft bone can be achieved by lateral bone-condensing technique, regardless of implant macro-design.",
publisher = "Wiley, Hoboken",
journal = "Clinical Implant Dentistry & Related Research",
title = "Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study",
volume = "15",
number = "3",
pages = "341-349",
doi = "10.1111/j.1708-8208.2011.00415.x"
}

Marković, A., Luis Calvo-Guirado, J., Lazić, Z., Gomez-Moreno, G., Ćalasan, D., Guardia, J., Čolić, S., Aguilar-Salvatierra, A., Gačić, B., Delgado-Ruiz, R., Janjić, B.,& Mišić, T.. (2013). Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study. in Clinical Implant Dentistry & Related Research
Wiley, Hoboken., 15(3), 341-349.
https://doi.org/10.1111/j.1708-8208.2011.00415.x

Marković A, Luis Calvo-Guirado J, Lazić Z, Gomez-Moreno G, Ćalasan D, Guardia J, Čolić S, Aguilar-Salvatierra A, Gačić B, Delgado-Ruiz R, Janjić B, Mišić T. Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study. in Clinical Implant Dentistry & Related Research. 2013;15(3):341-349.
doi:10.1111/j.1708-8208.2011.00415.x .

Marković, Aleksa, Luis Calvo-Guirado, Jose, Lazić, Zoran, Gomez-Moreno, Gerardo, Ćalasan, Dejan, Guardia, Javier, Čolić, Snježana, Aguilar-Salvatierra, Antonio, Gačić, Bojan, Delgado-Ruiz, Rafael, Janjić, Bojan, Mišić, Tijana, "Evaluation of Primary Stability of Self-Tapping and Non-Self-Tapping Dental Implants. A 12-Week Clinical Study" in Clinical Implant Dentistry & Related Research, 15, no. 3 (2013):341-349,
https://doi.org/10.1111/j.1708-8208.2011.00415.x . .