@article{
author = "Todorović, Tatjana and Dožić, Ivan and Vujanović, Dragana and Pejović, J. and Marjanović, Marjan",
year = "2005",
abstract = "In this paper, the influence of chronic lead intoxication on the activity of serum enzymes aspartate and alanine aminotransferases (AST and ALT) and alkaline phosphatase (ALP) was examined. The experiment was performed on 130 adult female DA rats and 80 young rats. Rats were treated by lead-acetate 100 and 30 mg Pb per kg body weight for 10, 20, 30, 40, 50 and 60 days. Young rats (offspring of studied female rats) were treated with lead only through the placenta and mother's milk. The activities of serum AST, ALT and ALP were determined spectrophotomerically by IFCC method. The activity of examined serum enzymes was significantly increased in conditions of chronic lead intoxication in female rats and their offspring in relation to the control group. The activity of serum AST, ALT and ALP was in a positive correlation with the time of intoxication. There were no significant differences between the activities of enzymes AST and ALT in the serum and the amount of lead. The activity of ALP was significantly higher in serum of rats treated with higher amounts of lead. Increased AST, ALT and ALP activity in serum is most likely the consequence of lead hepatotoxicity., U ovom radu su prikazani rezultati ispitivanja uticaja hroničnog trovanja olovom na aktivnost enzima aspartat i alanin aminotransferaze (AST i ALT) kao i alkalne fosfataze (ALP) u krvnom serumu pacova. Eksperiment je izveden na 130 ženki pacova i njihovih 80 mladunaca. Ženke su tretirane olovo-acetatom oralno putem pijaće vode u dve doze-100 i 30 mg/kg TM dnevno u trajanju od 10,20,30, 40, 50 i 60 dana, dok su njihovi mladunci olovo primali samo preko placente i putem mleka. Nakon žrtvovanja, od životinja je uzimana krv i u krvnom serumu je spektrofotometrijski, na automatskom analizatoru Hitachi 911, određivana aktivnost enzima AST, ALT i ALR Dobijeni rezultati su ukazali da hronično trovanje olovom ima za posledicu značajno povećanje aktivnosti enzima AST, ALT i ALP u krvnom serumu ženki pacova, pri čemu se aktivnost navedenih enzima linearno povećavala sa povećanjem vremena intoksikacije. Nisu zapažene značajne razlike u aktivnosti enzima AST i ALT u odnosu na primenjenu dozu olova, dok je u slučaju ALP ustanovljeno da veća doza olova ima za posledicu značajnije povećanje aktivnosti ovog enzima u krvnom serumu. U mladunaca ženki koje su tokom trudnoće i laktacije primale olovo, takođe je ustanovljeno značajno povećanje aktivnosti navedenih enzima u krvnom serumu u odnosu na kontrolnu grupu. Nisu ustanovljene značajne razlike u aktivnosti AST, ALT i ALP u serumu mladunaca u pogledu doze olova koje su njihove majke primale. Povećanje aktivnosti enzima AST, ALT i ALP u krvnom serumu ženki i mladunaca je najverovatnije posledica hepatotoksičnog dejstva olova udruženog sa intrahepatičkom holestazom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The influence of chronic lead poisoning on the activity of some serum enzymes in rats, Uticaj hroničnog trovanja olovom na aktivnost nekih enzima u krvnom serumu pacova",
volume = "55",
number = "5-6",
pages = "471-482",
doi = "10.2298/AVB0506471T"
}
