TY  - JOUR
AU  - Popović, Branka
AU  - Velimirović, Milica
AU  - Stojković, Tihomir
AU  - Brajović, Gavrilo
AU  - de Luka, Silvio R.
AU  - Milovanović, Ivan
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica
AU  - Ristic-Đurović, Jasna L.
AU  - Petronijević, Nataša
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2323
AB  - Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of ageing on the extrapineal melatonin synthetic pathway
VL  - 110
SP  - 151
EP  - 157
DO  - 10.1016/j.exger.2018.06.010
ER  - 

@article{
author = "Popović, Branka and Velimirović, Milica and Stojković, Tihomir and Brajović, Gavrilo and de Luka, Silvio R. and Milovanović, Ivan and Stefanović, Srđan and Nikolić, Dragica and Ristic-Đurović, Jasna L. and Petronijević, Nataša and Trbovich, Alexander M.",
year = "2018",
abstract = "Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of ageing on the extrapineal melatonin synthetic pathway",
volume = "110",
pages = "151-157",
doi = "10.1016/j.exger.2018.06.010"
}

Popović, B., Velimirović, M., Stojković, T., Brajović, G., de Luka, S. R., Milovanović, I., Stefanović, S., Nikolić, D., Ristic-Đurović, J. L., Petronijević, N.,& Trbovich, A. M.. (2018). The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 110, 151-157.
https://doi.org/10.1016/j.exger.2018.06.010

Popović B, Velimirović M, Stojković T, Brajović G, de Luka SR, Milovanović I, Stefanović S, Nikolić D, Ristic-Đurović JL, Petronijević N, Trbovich AM. The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology. 2018;110:151-157.
doi:10.1016/j.exger.2018.06.010 .

Popović, Branka, Velimirović, Milica, Stojković, Tihomir, Brajović, Gavrilo, de Luka, Silvio R., Milovanović, Ivan, Stefanović, Srđan, Nikolić, Dragica, Ristic-Đurović, Jasna L., Petronijević, Nataša, Trbovich, Alexander M., "The influence of ageing on the extrapineal melatonin synthetic pathway" in Experimental Gerontology, 110 (2018):151-157,
https://doi.org/10.1016/j.exger.2018.06.010 . .

TY  - JOUR
AU  - Stanković, Marija S.
AU  - Turuntas, Vladimir
AU  - de Luka, Silvio R.
AU  - Janković, Saša
AU  - Stefanović, Srđan
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2003
AB  - Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental & Molecular Pathology
T1  - Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation
VL  - 99
IS  - 3
SP  - 687
EP  - 692
DO  - 10.1016/j.yexmp.2015.11.016
ER  - 

@article{
author = "Stanković, Marija S. and Turuntas, Vladimir and de Luka, Silvio R. and Janković, Saša and Stefanović, Srđan and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
abstract = "Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental & Molecular Pathology",
title = "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation",
volume = "99",
number = "3",
pages = "687-692",
doi = "10.1016/j.yexmp.2015.11.016"
}

Stanković, M. S., Turuntas, V., de Luka, S. R., Janković, S., Stefanović, S., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 99(3), 687-692.
https://doi.org/10.1016/j.yexmp.2015.11.016

Stanković MS, Turuntas V, de Luka SR, Janković S, Stefanović S, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Trbovich AM. Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology. 2015;99(3):687-692.
doi:10.1016/j.yexmp.2015.11.016 .

Stanković, Marija S., Turuntas, Vladimir, de Luka, Silvio R., Janković, Saša, Stefanović, Srđan, Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation" in Experimental & Molecular Pathology, 99, no. 3 (2015):687-692,
https://doi.org/10.1016/j.yexmp.2015.11.016 . .