Petrović-Matić, Sanja

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Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction

Petrović-Matić, Sanja; Zelić, Ksenija; Milašin, Jelena; Popović, Branka; Pucar, Ana; Zelić, Obrad

(Srpsko lekarsko društvo, Beograd, 2014) 

TY  - JOUR
AU  - Petrović-Matić, Sanja
AU  - Zelić, Ksenija
AU  - Milašin, Jelena
AU  - Popović, Branka
AU  - Pucar, Ana
AU  - Zelić, Obrad
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1917
AB  - Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis.
AB  - Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction
T1  - Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze
VL  - 142
IS  - 5-6
SP  - 296
EP  - 300
DO  - 10.2298/SARH1406296M
ER  - 
@article{
author = "Petrović-Matić, Sanja and Zelić, Ksenija and Milašin, Jelena and Popović, Branka and Pucar, Ana and Zelić, Obrad",
year = "2014",
abstract = "Introduction Pathogenesis and some characteristics of periodontitis cannot be fully explained by bacterial etiology alone. Herpes viruses may bridge the gap between clinical characteristics and molecular understanding of periodontal destruction. Objective The aim of this study was to investigate the prevalence of herpes simplex virus type 1 (HSV-1) in gingival crevicular fluid (GCF) of healthy and damaged periodontium in Serbian population and to explore potential correlation between the presence of this virus and the level of periodontal destruction. Methods Samples were collected from gingival sulcus/periodontal pockets by sterile paper points and the presence of viral DNA in gingival crevicular fluid was assessed by PCR. Results There was no statistically significant difference in HSV-1 in presence between periodontitis patients (PG=38.9%) and healthy controls (HC=32.3%), (Chi-square test, with Yates' correction p=0.7574). However, HSV-1 positive patients showed significantly higher values of parameters of periodontal destruction (PPD=7.11±2.52, CAL=5.46±2.34) than periodontitis patients without HSV-1 in gingival crevicular fluid (PPD=4.70±1.79, CAL=3.39±2.65) (p values respectively, p=0.002 and p=0.023, Independent Samples T-Test). HSV-1 occurred more often in deeper (PPD≥6 mm) (69.2%) than in shallow pockets (3 mm lt PPD lt 6 mm) (18.2%) (Chi-square test, with Yates' correction, p=0.008). Plaque index was lower in the HSV-1 positive group (0.84± 0.69 vs. 1.43±0.76, p=0.023, Independent Samples T-Test). Conclusion This study demonstrated that the presence of HSV-1 in the gingival crevicular fluid coincides with a higher degree of tissue destruction in patients with periodontitis., Uvod Patogeneza i neke kliničke odlike parodontopatije ne mogu se do kraja objasniti bakterijskom etiologijom. Herpesvirusi bi mogli da premoste jaz koji postoji između kliničkih osobina i poznavanja patogenetskih mehanizama parodontopatije na molekularnom nivou. Cilj rada Cilj ove studije bio je određivanje prevalencije virusa herpes simplex tip 1 (HSV-1) u zdravom i obolelom parodoncijumu osoba u Srbiji, kao i utvrđivanje moguće korelacije između postojanja ovih virusa i stepena oštećenja parodoncijuma. Metode rada Uzorci su uzimani papirnim poenima iz gingivalnih sulkusa ili parodontalnih džepova, a virus je otkrivan reakcijom lančanog umnožavanja molekula DNK (engl. polymerase chain reaction - PCR). Rezultati Nije utvrđena statistički značajna razlika u prevalenciji HSV-1 između osoba s parodontopatijom (32,3%) i zdravim parodoncijumom (38,9%); χ2-test sa Jejtsovom (Yates) korekcijom: p=0,7574. U grupi ispitanika s parodontopatijom parametri koji označavaju stepen oštećenja (dubina parodontalnog džepa - DPDž; nivo pripojnog epitela - NPE) bili su značajno veći kod onih s otkrivenim virusom (DPDž: 7,11±2,52 mm; NPE: 5,46±2,34), nego kod ispitanika bez virusa u uzorcima gingivalne tečnosti (DPDž=4,70±1,79 mm; NPE=3,39±2,65); Studentov t-test za nevezane uzorke: p=0,002, odnosno p=0,023. U dubljim parodontalnim džepovima (DPDž≥6 mm) HSV-1 je otkriven statistički značajno češće (69,2%) nego u plićim džepovima (DPDž=3-6 mm) (18,2%); χ2-test sa Jejtsovom korekcijom: p=0,008. Prosečne vrednosti plak-indeksa bile su niže kod ispitanika sa HSV-1 (0,84±0,69) u poređenju sa ispitanicima kod kojih ovaj virus nije otkriven (1,43±0,76); Studentov t-test za nevezane uzorke: p=0,023. Zaključak Prikazana studija pokazala je da je postojanje HSV-1 povezano sa nivoom oštećenja tkiva kod osoba s parodontopatijom.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction, Otkrivanje virusa herpes simplex tip 1 u gingivalnoj tečnosti sulkusa ili parodontalnog džepa lančanom reakcijom polimeraze",
volume = "142",
number = "5-6",
pages = "296-300",
doi = "10.2298/SARH1406296M"
}
Petrović-Matić, S., Zelić, K., Milašin, J., Popović, B., Pucar, A.,& Zelić, O.. (2014). Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(5-6), 296-300.
https://doi.org/10.2298/SARH1406296M
Petrović-Matić S, Zelić K, Milašin J, Popović B, Pucar A, Zelić O. Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction. in Srpski arhiv za celokupno lekarstvo. 2014;142(5-6):296-300.
doi:10.2298/SARH1406296M .
Petrović-Matić, Sanja, Zelić, Ksenija, Milašin, Jelena, Popović, Branka, Pucar, Ana, Zelić, Obrad, "Detection of herpes simplex virus type 1 in gingival crevicular fluid of gingival sulcus/periodontal pocket using polymerase chain reaction" in Srpski arhiv za celokupno lekarstvo, 142, no. 5-6 (2014):296-300,
https://doi.org/10.2298/SARH1406296M . .
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