TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Popović, Branka
AU  - Puletić, Miljan
AU  - Kostić, Marija
AU  - Milašin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2155
AB  - Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.
AB  - Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Estimation of total bacteria by real-time PCR in patients with periodontal disease
T1  - Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom
VL  - 144
IS  - 1-2
SP  - 10
EP  - 14
DO  - 10.2298/SARH1602010B
ER  - 

@article{
author = "Brajović, Gavrilo and Popović, Branka and Puletić, Miljan and Kostić, Marija and Milašin, Jelena",
year = "2016",
abstract = "Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis., Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Estimation of total bacteria by real-time PCR in patients with periodontal disease, Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom",
volume = "144",
number = "1-2",
pages = "10-14",
doi = "10.2298/SARH1602010B"
}

Brajović, G., Popović, B., Puletić, M., Kostić, M.,& Milašin, J.. (2016). Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(1-2), 10-14.
https://doi.org/10.2298/SARH1602010B

Brajović G, Popović B, Puletić M, Kostić M, Milašin J. Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo. 2016;144(1-2):10-14.
doi:10.2298/SARH1602010B .

Brajović, Gavrilo, Popović, Branka, Puletić, Miljan, Kostić, Marija, Milašin, Jelena, "Estimation of total bacteria by real-time PCR in patients with periodontal disease" in Srpski arhiv za celokupno lekarstvo, 144, no. 1-2 (2016):10-14,
https://doi.org/10.2298/SARH1602010B . .

TY  - JOUR
AU  - Andrić, Miroslav
AU  - Nikolić, Nadja
AU  - Bosković, Marija
AU  - Miličić, Biljana
AU  - Skodrić, Sanja
AU  - Basta-Jovanović, Gordana
AU  - Milašin, Jelena
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1755
AB  - Several single nucleotide polymorphisms in survivin gene promoters, notably -31G/C, have been shown to modulate the expression and activity of the survivin protein. Consequently, the -31G/C polymorphism has been identified as a risk factor for the development of several types of tumors. The aim of this study was to investigate a possible association between the -31G/C polymorphism and the risk for keratocystic odontogenic tumor (KCOT) development. DNA from 52 biopsy specimens of KCOTs and from 82 buccal swabs of healthy individuals was subjected to PCR restriction fragment length polymorphism analysis to identify individual genotypes. The distribution of genotypes in KCOT and control groups, respectively, was: GG: 30 (57.7%) vs. 26 (31.7%); CG: 17 (32.7%) vs. 45 (54.9%); and CC: 5 (9.6%) vs. 11 (13.4%), respectively. These differences were statistically significant. The G allele was more common in the KCOT group than in the control group: 76 (74%) vs. 96 (59%), respectively. Logistic regression analysis showed that GC heterozygotes had a considerably decreased susceptibility for KCOTs compared with GG homozygotes. The same was true for GC+CC vs. GG. The GG genotype of the -31G/C polymorphism might be a risk factor for KCOT development.
PB  - Wiley-Blackwell, Hoboken
T2  - European Journal of Oral Sciences
T1  - Survivin gene promoter polymorphism-31G/C as a risk factor for keratocystic odontogenic tumor development
VL  - 120
IS  - 1
SP  - 9
EP  - 13
DO  - 10.1111/j.1600-0722.2011.00919.x
ER  - 

@article{
author = "Andrić, Miroslav and Nikolić, Nadja and Bosković, Marija and Miličić, Biljana and Skodrić, Sanja and Basta-Jovanović, Gordana and Milašin, Jelena",
year = "2012",
abstract = "Several single nucleotide polymorphisms in survivin gene promoters, notably -31G/C, have been shown to modulate the expression and activity of the survivin protein. Consequently, the -31G/C polymorphism has been identified as a risk factor for the development of several types of tumors. The aim of this study was to investigate a possible association between the -31G/C polymorphism and the risk for keratocystic odontogenic tumor (KCOT) development. DNA from 52 biopsy specimens of KCOTs and from 82 buccal swabs of healthy individuals was subjected to PCR restriction fragment length polymorphism analysis to identify individual genotypes. The distribution of genotypes in KCOT and control groups, respectively, was: GG: 30 (57.7%) vs. 26 (31.7%); CG: 17 (32.7%) vs. 45 (54.9%); and CC: 5 (9.6%) vs. 11 (13.4%), respectively. These differences were statistically significant. The G allele was more common in the KCOT group than in the control group: 76 (74%) vs. 96 (59%), respectively. Logistic regression analysis showed that GC heterozygotes had a considerably decreased susceptibility for KCOTs compared with GG homozygotes. The same was true for GC+CC vs. GG. The GG genotype of the -31G/C polymorphism might be a risk factor for KCOT development.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "European Journal of Oral Sciences",
title = "Survivin gene promoter polymorphism-31G/C as a risk factor for keratocystic odontogenic tumor development",
volume = "120",
number = "1",
pages = "9-13",
doi = "10.1111/j.1600-0722.2011.00919.x"
}

Andrić, M., Nikolić, N., Bosković, M., Miličić, B., Skodrić, S., Basta-Jovanović, G.,& Milašin, J.. (2012). Survivin gene promoter polymorphism-31G/C as a risk factor for keratocystic odontogenic tumor development. in European Journal of Oral Sciences
Wiley-Blackwell, Hoboken., 120(1), 9-13.
https://doi.org/10.1111/j.1600-0722.2011.00919.x

Andrić M, Nikolić N, Bosković M, Miličić B, Skodrić S, Basta-Jovanović G, Milašin J. Survivin gene promoter polymorphism-31G/C as a risk factor for keratocystic odontogenic tumor development. in European Journal of Oral Sciences. 2012;120(1):9-13.
doi:10.1111/j.1600-0722.2011.00919.x .

Andrić, Miroslav, Nikolić, Nadja, Bosković, Marija, Miličić, Biljana, Skodrić, Sanja, Basta-Jovanović, Gordana, Milašin, Jelena, "Survivin gene promoter polymorphism-31G/C as a risk factor for keratocystic odontogenic tumor development" in European Journal of Oral Sciences, 120, no. 1 (2012):9-13,
https://doi.org/10.1111/j.1600-0722.2011.00919.x . .