TY  - JOUR
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - de Luka, Silvio R.
AU  - Dragović, Gordana
AU  - Jevtović, Đorđe
AU  - Maslać, Aleksandar
AU  - Ristić-Đurović, Jasna
AU  - Trbovich, Alexander M.
PY  - 2023
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3281
AB  - We appraised the relationship between the biological and the chrono-
logical age and estimated the rate of biological aging in HIV-infected patients. Two
independent biomarkers, the relative telomere length and iron metabolism parame-
ters, were analyzed in younger (,35) and older (.50) HIV-infected and uninfected
patients (control group). In our control group, telomeres of younger patients were
significantly longer than telomeres of older ones. However, in HIV-infected partici-
pants, the difference in the length of telomeres was lost. By combining the length of
telomeres with serum iron, ferritin, and transferrin iron-binding capacity, a new for-
mula for determination of the aging process was developed. The life expectancy of
the healthy population was related to their biological age, and HIV-infected patients
were biologically older. The effect of antiretroviral HIV drug therapies varied with
respect to the biological aging process.
IMPORTANCE This article is focused on the dynamics of human aging. Moreover, its
interdisciplinary approach is applicable to various systems that are aging.
PB  - American Society for Microbiology
T2  - Microbiology Spectrum
T1  - HIV-Infected Patients as a Model of Aging
VL  - 11
IS  - 3
SP  - e00532-23
DO  - 10.1128/spectrum.00532-23
ER  - 

@article{
author = "Toljić, Boško and Milašin, Jelena and de Luka, Silvio R. and Dragović, Gordana and Jevtović, Đorđe and Maslać, Aleksandar and Ristić-Đurović, Jasna and Trbovich, Alexander M.",
year = "2023",
abstract = "We appraised the relationship between the biological and the chrono-
logical age and estimated the rate of biological aging in HIV-infected patients. Two
independent biomarkers, the relative telomere length and iron metabolism parame-
ters, were analyzed in younger (,35) and older (.50) HIV-infected and uninfected
patients (control group). In our control group, telomeres of younger patients were
significantly longer than telomeres of older ones. However, in HIV-infected partici-
pants, the difference in the length of telomeres was lost. By combining the length of
telomeres with serum iron, ferritin, and transferrin iron-binding capacity, a new for-
mula for determination of the aging process was developed. The life expectancy of
the healthy population was related to their biological age, and HIV-infected patients
were biologically older. The effect of antiretroviral HIV drug therapies varied with
respect to the biological aging process.
IMPORTANCE This article is focused on the dynamics of human aging. Moreover, its
interdisciplinary approach is applicable to various systems that are aging.",
publisher = "American Society for Microbiology",
journal = "Microbiology Spectrum",
title = "HIV-Infected Patients as a Model of Aging",
volume = "11",
number = "3",
pages = "e00532-23",
doi = "10.1128/spectrum.00532-23"
}

Toljić, B., Milašin, J., de Luka, S. R., Dragović, G., Jevtović, Đ., Maslać, A., Ristić-Đurović, J.,& Trbovich, A. M.. (2023). HIV-Infected Patients as a Model of Aging. in Microbiology Spectrum
American Society for Microbiology., 11(3), e00532-23.
https://doi.org/10.1128/spectrum.00532-23

Toljić B, Milašin J, de Luka SR, Dragović G, Jevtović Đ, Maslać A, Ristić-Đurović J, Trbovich AM. HIV-Infected Patients as a Model of Aging. in Microbiology Spectrum. 2023;11(3):e00532-23.
doi:10.1128/spectrum.00532-23 .

Toljić, Boško, Milašin, Jelena, de Luka, Silvio R., Dragović, Gordana, Jevtović, Đorđe, Maslać, Aleksandar, Ristić-Đurović, Jasna, Trbovich, Alexander M., "HIV-Infected Patients as a Model of Aging" in Microbiology Spectrum, 11, no. 3 (2023):e00532-23,
https://doi.org/10.1128/spectrum.00532-23 . .

TY  - JOUR
AU  - Dragović, Gordana
AU  - Toljić, Boško
AU  - Jevtović, Đorđe
AU  - Lukić, Relja
AU  - de Luka, Silvio R.
AU  - Trbovich, Alexander
AU  - Milašin, Jelena
PY  - 2021
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/3285
AB  - Background: Components of the metabolic syndrome (MetS) play an important role in the accelerated aging process. Relative telomere length (RTL) is a marker of biological aging. The aim of our study was to determine RTL and its possible association with MetS and the components of MetS in HIV-infected patients treated with cART.

Methods: We included 24 HIV-infected men, all Caucasians, with successful cART (<50 HIV-RNA copies/mL) and on stable cART for at least 24 months. The presence of MetS and its components was determined by the criteria prescribed by the International Diabetes Federation. RTL was determined by Real-Time PCR and ΔΔCt method. We performed a multiple linear regression modeling on log-transformed RTL (dependant variable) to evaluate which components of the metabolic syndrome as well as cART duration and cART type, had an impact on RTL.

Results: Eleven (45.8%) patients had and 13 (54.2%) had not MetS. All patients, had an undetectable viral RNA and a relatively good immune status. The mean RTL was 0.62 ± 0.15 and 0.95 ± 0.36 in patients with and without MetS, respectively (p = 0.01). Multiple linear regression model showed no significant association between duration of cART, cART type and RTL (p = 0.2165, p = 0.8628, respectively). The same analysis showed that an increase in number of MetS components was associated with shorter telomere length (β = -0.4982, p = 0.042).

Conclusions: We showed for the first time association between RTL shortening in HIV-infected men with metabolic syndrome. Furthermore, our study also indicated that an increment of metabolic syndrome components is strongly associated with shorter telomere length.
PB  - Elsevier Inc.
T2  - Experimental Gerontology
T1  - Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy
VL  - 147
IS  - May
SP  - 111269
DO  - 10.1016/j.exger.2021.111269
ER  - 

@article{
author = "Dragović, Gordana and Toljić, Boško and Jevtović, Đorđe and Lukić, Relja and de Luka, Silvio R. and Trbovich, Alexander and Milašin, Jelena",
year = "2021",
abstract = "Background: Components of the metabolic syndrome (MetS) play an important role in the accelerated aging process. Relative telomere length (RTL) is a marker of biological aging. The aim of our study was to determine RTL and its possible association with MetS and the components of MetS in HIV-infected patients treated with cART.

Methods: We included 24 HIV-infected men, all Caucasians, with successful cART (<50 HIV-RNA copies/mL) and on stable cART for at least 24 months. The presence of MetS and its components was determined by the criteria prescribed by the International Diabetes Federation. RTL was determined by Real-Time PCR and ΔΔCt method. We performed a multiple linear regression modeling on log-transformed RTL (dependant variable) to evaluate which components of the metabolic syndrome as well as cART duration and cART type, had an impact on RTL.

Results: Eleven (45.8%) patients had and 13 (54.2%) had not MetS. All patients, had an undetectable viral RNA and a relatively good immune status. The mean RTL was 0.62 ± 0.15 and 0.95 ± 0.36 in patients with and without MetS, respectively (p = 0.01). Multiple linear regression model showed no significant association between duration of cART, cART type and RTL (p = 0.2165, p = 0.8628, respectively). The same analysis showed that an increase in number of MetS components was associated with shorter telomere length (β = -0.4982, p = 0.042).

Conclusions: We showed for the first time association between RTL shortening in HIV-infected men with metabolic syndrome. Furthermore, our study also indicated that an increment of metabolic syndrome components is strongly associated with shorter telomere length.",
publisher = "Elsevier Inc.",
journal = "Experimental Gerontology",
title = "Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy",
volume = "147",
number = "May",
pages = "111269",
doi = "10.1016/j.exger.2021.111269"
}

Dragović, G., Toljić, B., Jevtović, Đ., Lukić, R., de Luka, S. R., Trbovich, A.,& Milašin, J.. (2021). Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy. in Experimental Gerontology
Elsevier Inc.., 147(May), 111269.
https://doi.org/10.1016/j.exger.2021.111269

Dragović G, Toljić B, Jevtović Đ, Lukić R, de Luka SR, Trbovich A, Milašin J. Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy. in Experimental Gerontology. 2021;147(May):111269.
doi:10.1016/j.exger.2021.111269 .

Dragović, Gordana, Toljić, Boško, Jevtović, Đorđe, Lukić, Relja, de Luka, Silvio R., Trbovich, Alexander, Milašin, Jelena, "Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy" in Experimental Gerontology, 147, no. May (2021):111269,
https://doi.org/10.1016/j.exger.2021.111269 . .

TY  - JOUR
AU  - Oprić, D.
AU  - Stankovich, A.D.
AU  - Nenadović, A.
AU  - Kovačević, S.
AU  - Obradović, D.D.
AU  - de Luka, Silvio R.
AU  - Nešović-Ostojić, J.
AU  - Milašin, Jelena
AU  - Ilić, A.Ž.
AU  - Trbovich, Alexander M.
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2506
AB  - Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.
PB  - Elsevier Ltd
T2  - Biomedical Signal Processing & Control
T1  - Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils
VL  - 61
DO  - 10.1016/j.bspc.2020.101959
ER  - 

@article{
author = "Oprić, D. and Stankovich, A.D. and Nenadović, A. and Kovačević, S. and Obradović, D.D. and de Luka, Silvio R. and Nešović-Ostojić, J. and Milašin, Jelena and Ilić, A.Ž. and Trbovich, Alexander M.",
year = "2020",
abstract = "Objective: Inflammation is a biological response of tissue to harmful stimuli. A high-fat diet was linked to low-grade chronic liver inflammation, which can further lead to more severe health conditions. It is crucial to assess the intensity of inflammation and structural tissue changes to reduce the subjective judgment by the examiner. We propose fractal-based methods for early-stage low-degree liver inflammation grading. Methods: We have randomly divided 40 C57BL/6 female mice into four groups (control, linseed oil, palm oil, sunflower oil). After 100 days, animals were euthanized, and liver tissue collected for analyses. We performed calculations of fractal dimension, fractal lacunarity, multifractal spectra, local fractal dimension, and particle metrics, applicable to tissue segmentation and grading. Results: Pathohistological analysis of some liver tissue showed a low-grade inflammatory infiltrate around the portal vein of experimental groups subjected to different high-fat diets. Differences in fractal dimension and lacunarity of the inflamed tissue were, in most cases, statistically significant between the high-fat diet groups. Both the observed intensity and area of inflammation were lowest for the sunflower oil. The results of standard fractal analysis, local fractal analysis, and particle analysis were in an excellent agreement. Conclusions: This study demonstrated the efficiency of the fractal analysis based tools in the quantification of complexity and early-stage structural changes in inflamed liver tissue, which could potentially be used in the diagnostic workup of inflammation in the liver. The presented methods could be implemented within a wider scope computer-aided diagnostics system in a very straightforward manner.",
publisher = "Elsevier Ltd",
journal = "Biomedical Signal Processing & Control",
title = "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils",
volume = "61",
doi = "10.1016/j.bspc.2020.101959"
}

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, S. R., Nešović-Ostojić, J., Milašin, J., Ilić, A.Ž.,& Trbovich, A. M.. (2020). Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control
Elsevier Ltd., 61.
https://doi.org/10.1016/j.bspc.2020.101959

Oprić D, Stankovich A, Nenadović A, Kovačević S, Obradović D, de Luka SR, Nešović-Ostojić J, Milašin J, Ilić A, Trbovich AM. Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils. in Biomedical Signal Processing & Control. 2020;61.
doi:10.1016/j.bspc.2020.101959 .

Oprić, D., Stankovich, A.D., Nenadović, A., Kovačević, S., Obradović, D.D., de Luka, Silvio R., Nešović-Ostojić, J., Milašin, Jelena, Ilić, A.Ž., Trbovich, Alexander M., "Fractal analysis tools for early assessment of liver inflammation induced by chronic consumption of linseed, palm and sunflower oils" in Biomedical Signal Processing & Control, 61 (2020),
https://doi.org/10.1016/j.bspc.2020.101959 . .

TY  - CONF
AU  - Dragović, Gordana
AU  - Dimitrijević, B.
AU  - Toljić, Boško
AU  - Milašin, Jelena
AU  - de Luka, Silvio R.
AU  - Jevtović, Đorđe
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2314
PB  - John Wiley & Sons Ltd, Chichester
C3  - Journal of the International Aids Society
T1  - Disturbance in iron metabolism parameters: a possible marker of preterm ageing in HIV-infected males
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_smile_2314
ER  - 

@conference{
author = "Dragović, Gordana and Dimitrijević, B. and Toljić, Boško and Milašin, Jelena and de Luka, Silvio R. and Jevtović, Đorđe and Trbovich, Alexander M.",
year = "2018",
publisher = "John Wiley & Sons Ltd, Chichester",
journal = "Journal of the International Aids Society",
title = "Disturbance in iron metabolism parameters: a possible marker of preterm ageing in HIV-infected males",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_smile_2314"
}

Dragović, G., Dimitrijević, B., Toljić, B., Milašin, J., de Luka, S. R., Jevtović, Đ.,& Trbovich, A. M.. (2018). Disturbance in iron metabolism parameters: a possible marker of preterm ageing in HIV-infected males. in Journal of the International Aids Society
John Wiley & Sons Ltd, Chichester., 21.
https://hdl.handle.net/21.15107/rcub_smile_2314

Dragović G, Dimitrijević B, Toljić B, Milašin J, de Luka SR, Jevtović Đ, Trbovich AM. Disturbance in iron metabolism parameters: a possible marker of preterm ageing in HIV-infected males. in Journal of the International Aids Society. 2018;21.
https://hdl.handle.net/21.15107/rcub_smile_2314 .

Dragović, Gordana, Dimitrijević, B., Toljić, Boško, Milašin, Jelena, de Luka, Silvio R., Jevtović, Đorđe, Trbovich, Alexander M., "Disturbance in iron metabolism parameters: a possible marker of preterm ageing in HIV-infected males" in Journal of the International Aids Society, 21 (2018),
https://hdl.handle.net/21.15107/rcub_smile_2314 .

TY  - JOUR
AU  - Popović, Branka
AU  - Velimirović, Milica
AU  - Stojković, Tihomir
AU  - Brajović, Gavrilo
AU  - de Luka, Silvio R.
AU  - Milovanović, Ivan
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica
AU  - Ristic-Đurović, Jasna L.
AU  - Petronijević, Nataša
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2323
AB  - Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of ageing on the extrapineal melatonin synthetic pathway
VL  - 110
SP  - 151
EP  - 157
DO  - 10.1016/j.exger.2018.06.010
ER  - 

@article{
author = "Popović, Branka and Velimirović, Milica and Stojković, Tihomir and Brajović, Gavrilo and de Luka, Silvio R. and Milovanović, Ivan and Stefanović, Srđan and Nikolić, Dragica and Ristic-Đurović, Jasna L. and Petronijević, Nataša and Trbovich, Alexander M.",
year = "2018",
abstract = "Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of ageing on the extrapineal melatonin synthetic pathway",
volume = "110",
pages = "151-157",
doi = "10.1016/j.exger.2018.06.010"
}

Popović, B., Velimirović, M., Stojković, T., Brajović, G., de Luka, S. R., Milovanović, I., Stefanović, S., Nikolić, D., Ristic-Đurović, J. L., Petronijević, N.,& Trbovich, A. M.. (2018). The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 110, 151-157.
https://doi.org/10.1016/j.exger.2018.06.010

Popović B, Velimirović M, Stojković T, Brajović G, de Luka SR, Milovanović I, Stefanović S, Nikolić D, Ristic-Đurović JL, Petronijević N, Trbovich AM. The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology. 2018;110:151-157.
doi:10.1016/j.exger.2018.06.010 .

Popović, Branka, Velimirović, Milica, Stojković, Tihomir, Brajović, Gavrilo, de Luka, Silvio R., Milovanović, Ivan, Stefanović, Srđan, Nikolić, Dragica, Ristic-Đurović, Jasna L., Petronijević, Nataša, Trbovich, Alexander M., "The influence of ageing on the extrapineal melatonin synthetic pathway" in Experimental Gerontology, 110 (2018):151-157,
https://doi.org/10.1016/j.exger.2018.06.010 . .

TY  - JOUR
AU  - Toljić, Boško
AU  - Trbovich, Alexander M.
AU  - Matić-Petrović, Sanja
AU  - Kannosh, Ibrahim
AU  - Dragović, Gordana
AU  - Jevtović, Đorđe
AU  - de Luka, Silvio R.
AU  - Ristic-Đurović, Jasna L.
AU  - Milašin, Jelena
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2269
AB  - The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.
PB  - Luigi Ponzio e figlio Editori
T2  - New Microbiologica
T1  - Ageing with HIV - a periodontal perspective
VL  - 41
IS  - 1
SP  - 61
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_smile_2269
ER  - 

@article{
author = "Toljić, Boško and Trbovich, Alexander M. and Matić-Petrović, Sanja and Kannosh, Ibrahim and Dragović, Gordana and Jevtović, Đorđe and de Luka, Silvio R. and Ristic-Đurović, Jasna L. and Milašin, Jelena",
year = "2018",
abstract = "The importance of oral microflora composition in HIV-infected patients is well recognized. However, no studies so far have dealt with age-related changes in periodontal pathogens occurrence in HIV+ individuals. The aim of the present study was to assess and compare temporal changes of bacteria frequency in younger ( lt = 35 years) and older (>= 50 years) HIV-infected and non-infected individuals. Bacterial DNA was isolated from buccal swabs of 30 younger and 30 older subjects in both HIV+ and HIV-groups. By means of PCR the following microorganisms were detected: Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Peptostreptococcus micros, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola. Oral and periodontal examinations were performed in all subjects. The prevalence of microorganisms was significantly higher in HIV+ patients compared to controls, and their distribution showed a notable shift. The decreasing incidence in HIV-subjects was: Pi>Pm>Pg>Aa>Ec>Tf>Td whilst in HIV+ it was: Pi>Pm>Ec>Pg>Tf>Aa>Td. Oral manifestations of HIV infection were more frequent in older compared to younger patients. All measured values of clinical periodontal parameters were significantly higher in older compared to younger HIV+ patients. Ageing in HIV+ subjects is accompanied with a substantial increase and rearrangements of periodontal microflora, potentially aggravating oral and systemic health.",
publisher = "Luigi Ponzio e figlio Editori",
journal = "New Microbiologica",
title = "Ageing with HIV - a periodontal perspective",
volume = "41",
number = "1",
pages = "61-66",
url = "https://hdl.handle.net/21.15107/rcub_smile_2269"
}

Toljić, B., Trbovich, A. M., Matić-Petrović, S., Kannosh, I., Dragović, G., Jevtović, Đ., de Luka, S. R., Ristic-Đurović, J. L.,& Milašin, J.. (2018). Ageing with HIV - a periodontal perspective. in New Microbiologica
Luigi Ponzio e figlio Editori., 41(1), 61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269

Toljić B, Trbovich AM, Matić-Petrović S, Kannosh I, Dragović G, Jevtović Đ, de Luka SR, Ristic-Đurović JL, Milašin J. Ageing with HIV - a periodontal perspective. in New Microbiologica. 2018;41(1):61-66.
https://hdl.handle.net/21.15107/rcub_smile_2269 .

Toljić, Boško, Trbovich, Alexander M., Matić-Petrović, Sanja, Kannosh, Ibrahim, Dragović, Gordana, Jevtović, Đorđe, de Luka, Silvio R., Ristic-Đurović, Jasna L., Milašin, Jelena, "Ageing with HIV - a periodontal perspective" in New Microbiologica, 41, no. 1 (2018):61-66,
https://hdl.handle.net/21.15107/rcub_smile_2269 .

TY  - JOUR
AU  - Stanković, Marija
AU  - Pantić, Igor
AU  - de Luka, Silvio R.
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Pantić, Senka
AU  - Trbovich, Alexander M.
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2129
AB  - The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.
PB  - Wiley, Hoboken
T2  - Journal of Microscopy
T1  - Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation
VL  - 261
IS  - 3
SP  - 277
EP  - 284
DO  - 10.1111/jmi.12330
ER  - 

@article{
author = "Stanković, Marija and Pantić, Igor and de Luka, Silvio R. and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Pantić, Senka and Trbovich, Alexander M.",
year = "2016",
abstract = "The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Microscopy",
title = "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation",
volume = "261",
number = "3",
pages = "277-284",
doi = "10.1111/jmi.12330"
}

Stanković, M., Pantić, I., de Luka, S. R., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S., Pantić, S.,& Trbovich, A. M.. (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy
Wiley, Hoboken., 261(3), 277-284.
https://doi.org/10.1111/jmi.12330

Stanković M, Pantić I, de Luka SR, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Pantić S, Trbovich AM. Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. in Journal of Microscopy. 2016;261(3):277-284.
doi:10.1111/jmi.12330 .

Stanković, Marija, Pantić, Igor, de Luka, Silvio R., Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Pantić, Senka, Trbovich, Alexander M., "Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation" in Journal of Microscopy, 261, no. 3 (2016):277-284,
https://doi.org/10.1111/jmi.12330 . .

TY  - CONF
AU  - Stanković, Marija
AU  - Pantić, I
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - de Luka, Silvio R.
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2006
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy
T1  - The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation
VL  - 70
SP  - 188
EP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_smile_2006
ER  - 

@conference{
author = "Stanković, Marija and Pantić, I and Puškaš, Nela and Zaletel, Ivan and de Luka, Silvio R. and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy",
title = "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation",
volume = "70",
pages = "188-188",
url = "https://hdl.handle.net/21.15107/rcub_smile_2006"
}

Stanković, M., Pantić, I., Puškaš, N., Zaletel, I., de Luka, S. R., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy
Wiley-Blackwell, Hoboken., 70, 188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006

Stanković M, Pantić I, Puškaš N, Zaletel I, de Luka SR, Milutinović-Smiljanić S, Trbovich AM. The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation. in Allergy. 2015;70:188-188.
https://hdl.handle.net/21.15107/rcub_smile_2006 .

Stanković, Marija, Pantić, I, Puškaš, Nela, Zaletel, Ivan, de Luka, Silvio R., Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "The role of IL-33/ST2 axis in enhancing inflammation, tissue damage and structural changes in acute inflammation" in Allergy, 70 (2015):188-188,
https://hdl.handle.net/21.15107/rcub_smile_2006 .

TY  - JOUR
AU  - Stanković, Marija S.
AU  - Turuntas, Vladimir
AU  - de Luka, Silvio R.
AU  - Janković, Saša
AU  - Stefanović, Srđan
AU  - Puškaš, Nela
AU  - Zaletel, Ivan
AU  - Milutinović-Smiljanić, Sanja
AU  - Trbovich, Alexander M.
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2003
AB  - Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental & Molecular Pathology
T1  - Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation
VL  - 99
IS  - 3
SP  - 687
EP  - 692
DO  - 10.1016/j.yexmp.2015.11.016
ER  - 

@article{
author = "Stanković, Marija S. and Turuntas, Vladimir and de Luka, Silvio R. and Janković, Saša and Stefanović, Srđan and Puškaš, Nela and Zaletel, Ivan and Milutinović-Smiljanić, Sanja and Trbovich, Alexander M.",
year = "2015",
abstract = "Aim: The aim of this study was to examine the role of the IL-33/ST2 pathway in pathogenesis of acute inflammation by investigating its possible role in alteration of iron and hematological parameters in experimental model of acute inflammation. Material and methods: Wild-type and ST2 knockout BALB/c mice were divided into four groups: wild-type control group, ST2-/- control group, wild-type inflammatory group, and ST2-/- inflammatory group. Acute inflammation was induced by intramuscular injection of turpentine oil, while control groups were injected with saline. After 12 h animals were anesthetized, and the treated tissue, blood and spleen were collected. Iron concentration in the treated tissue, hemoglobin blood concentration, mean corpuscular hemoglobin (MCH), hematocrit, erythrocyte, neutrophil and lymphocyte blood count, and erythrocytes percentage in spleen were determined. Results: Iron concentration in the treated tissue was significantly higher in wild-type inflammatory group (WT-I) when compared to both, the wild-type control group (WT-C) and ST2-/- inflammatory group (KO-I). There was no significant difference in iron concentration between ST2-/- control group (KO-C) and the KO-I. MCH had significantly decreased in WT-I when compared to WT-C, while there was no significant difference between KO-C and KO-I. Hemoglobin blood concentration significantly increased in KO-I in comparison to KO-C, while it did not significantly differ between WT-I and KO-I. Erythrocyte count and hematocrit had significantly increased, while the percentage of erythrocytes in spleen decreased in both inflammatory groups when compared to their controls. Neutrophil count significantly decreased in WT-I, when compared to WT-C. Lymphocyte count decreased in both inflammatory groups when compared to their controls. Conclusion: Results of this study indicate that the IL-33/ST2 axis could have a role in the alteration of iron in acute inflammation, namely in an increase of iron concentration at the site of acute inflammation and a decrease of blood mean corpuscular hemoglobin.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental & Molecular Pathology",
title = "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation",
volume = "99",
number = "3",
pages = "687-692",
doi = "10.1016/j.yexmp.2015.11.016"
}

Stanković, M. S., Turuntas, V., de Luka, S. R., Janković, S., Stefanović, S., Puškaš, N., Zaletel, I., Milutinović-Smiljanić, S.,& Trbovich, A. M.. (2015). Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 99(3), 687-692.
https://doi.org/10.1016/j.yexmp.2015.11.016

Stanković MS, Turuntas V, de Luka SR, Janković S, Stefanović S, Puškaš N, Zaletel I, Milutinović-Smiljanić S, Trbovich AM. Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation. in Experimental & Molecular Pathology. 2015;99(3):687-692.
doi:10.1016/j.yexmp.2015.11.016 .

Stanković, Marija S., Turuntas, Vladimir, de Luka, Silvio R., Janković, Saša, Stefanović, Srđan, Puškaš, Nela, Zaletel, Ivan, Milutinović-Smiljanić, Sanja, Trbovich, Alexander M., "Effects of Il-33/St2 pathway on alteration of iron and hematological parameters in acute inflammation" in Experimental & Molecular Pathology, 99, no. 3 (2015):687-692,
https://doi.org/10.1016/j.yexmp.2015.11.016 . .