TY  - JOUR
AU  - Čolović, Božana
AU  - Janković, Ognjenka
AU  - Živković, Slavoljub
AU  - Zizak, Zeljko
AU  - Zizak, Irina Besu
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2442
AB  - The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.
PB  - Elsevier Sci Ltd, Oxford
T2  - Ceramics International
T1  - A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
VL  - 45
IS  - 7
SP  - 9211
EP  - 9218
DO  - 10.1016/j.ceramint.2019.01.266
ER  - 

@article{
author = "Čolović, Božana and Janković, Ognjenka and Živković, Slavoljub and Zizak, Zeljko and Zizak, Irina Besu and Jokanović, Vukoman",
year = "2019",
abstract = "The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Ceramics International",
title = "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis",
volume = "45",
number = "7",
pages = "9211-9218",
doi = "10.1016/j.ceramint.2019.01.266"
}

Čolović, B., Janković, O., Živković, S., Zizak, Z., Zizak, I. B.,& Jokanović, V.. (2019). A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International
Elsevier Sci Ltd, Oxford., 45(7), 9211-9218.
https://doi.org/10.1016/j.ceramint.2019.01.266

Čolović B, Janković O, Živković S, Zizak Z, Zizak IB, Jokanović V. A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International. 2019;45(7):9211-9218.
doi:10.1016/j.ceramint.2019.01.266 .

Čolović, Božana, Janković, Ognjenka, Živković, Slavoljub, Zizak, Zeljko, Zizak, Irina Besu, Jokanović, Vukoman, "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis" in Ceramics International, 45, no. 7 (2019):9211-9218,
https://doi.org/10.1016/j.ceramint.2019.01.266 . .

TY  - JOUR
AU  - Paras, S.
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Mitrović-Ajtić, O.
AU  - Dekić, R.
AU  - Soldatović, Ivan
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2393
AB  - Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
VL  - 52
IS  - 8
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 

@article{
author = "Paras, S. and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana and Mitrović-Ajtić, O. and Dekić, R. and Soldatović, Ivan and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman",
year = "2019",
abstract = "Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
volume = "52",
number = "8",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}

Paras, S., Janković, O., Trišić, D., Čolović, B., Mitrović-Ajtić, O., Dekić, R., Soldatović, I., Živković-Sandić, M., Živković, S.,& Jokanović, V.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal
Wiley, Hoboken., 52(8), 1162-1172.
https://doi.org/10.1111/iej.13105

Paras S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković-Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;52(8):1162-1172.
doi:10.1111/iej.13105 .

Paras, S., Janković, Ognjenka, Trišić, Dijana, Čolović, Božana, Mitrović-Ajtić, O., Dekić, R., Soldatović, Ivan, Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman, "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal, 52, no. 8 (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .

TY  - JOUR
AU  - Janković, Ognjenka
AU  - Paraš, Smiljana
AU  - Tadić-Latinović, Ljiljana
AU  - Josipović, Renata
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2352
AB  - Introduction/Objective The aim of this paper was to verify the biocompatibility of the newly synthesized nanostructured material based on calcium aluminate after implantation into the subcutaneous tissue of rats. Methods The study included 18 rats aged 10-11 weeks, divided into two experimental groups (n = 9). In all animals, incision took place on the back and two pockets of 15 mm in depth were made, in which sterile polyethylene tubes with test materials [calcium aluminate cement (ALBO-CA), calcium silicate cement with the addition of hydroxyapatite (ALBO-CSHA), and mineral trioxide aggregate (MTA) for the control group) were implanted. Six rats of each group were sacrificed in three observational periods (seven, 15, 30 days). Pathohistological analysis included inflammation, bleeding, fibrous capsule, and tissue integrity around the implanted material. Results After seven days of treatment, ALBO-CA and ALBO-CSHA showed better tissue response compared to MTA, with a statistically significant difference in inflammation intensity (p = 0.2781). The difference in vascular congestion and thickness of the fibrous capsule after implantation of ALBO-CA material compared to MTA was also statistically significant (p = 0.5567). At the end of the 30-day evaluation period, an identical inflammatory response of connective tissue at the site of implanting ALBO-CA, ALBO-CSHA, and MTA (score of 0.7) was recorded. The formation of thick or moderately thick fibrous capsule was found to be the thickest in ALBO-CA (grade 3.7). There were no statistically significant differences between the parameters analyzed after 30 days. Conclusion Newly synthesized ALBO-CA showed a satisfactory tissue response and confirmed biocompatibility after implantation in subcutaneous tissue of rats.
AB  - Uvod/Cilj Cilj ovog rada je bio da se proveri biokompatibilnost novosintetisanog nanostrukturnog materijala na bazi kalcijum-aluminata posle implantacije u potkožno tkivo pacova. Metode U istraživanje je uključeno 18 pacova starosti 10-11 sedmica, koji su podeljeni u dve eksperimentalne grupe (n = 9). Kod svih životinja je urađena incizija na leđima i formirana su dva džepa dubine 15 mm u koja su aplicirane sterilne polietilenske tubice sa testnim materijalima (ALBO-CA, ALBO-CSHA i kontrola MTA). Po šest pacova svake grupe je žrtvovano u tri opservaciona perioda (7, 15 i 30 dana). Patohistološki su analizirani inflamacija, krvarenje, fibrozna kapsula i integritet tkiva oko implantiranog materijala. Rezultati Posle sedam dana ALBO-CA i ALBO-CSHA su pokazali bolji tkivni odgovor u odnosu na MTA sa statistički značajnom razlikom u intenzitetu inflamacije (p = 0,2781). Razlika u vaskularnoj kongestiji i debljini fibrozne kapsule posle implantacije materijala ALBO-CA u odnosu na MTA je takođe bila statistički značajna (p = 0,5567). Na kraju evaluacionog perioda od 30 dana zabeležen je identičan inflamatorni odgovor rastresitog vezivnog tkiva na mestu implantacije ALBO-CA, ALBO-CSHA i MTA (ocena 0,7). Konstatovano je formiranje debele ili umereno debele fibrozne kapsule, koja je bila najdeblja kod ALBO-CA (ocena 3,7). Statistički značajne razlike između analiziranih parametara posle 30 dana nije bilo. Zaključak Novosintetisani ALBO-CA je pokazao zadovoljavajuću tkivnu reakciju i potvrdio biokompatibilnost posle implantacije u potkožno tkivo pacova.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Biocompatibility of nanostructured biomaterials based on calcium aluminate
T1  - Biokompatibilnost nanostrukturnih biomaterijala na bazi kalcijum-aluminata
VL  - 146
IS  - 11-12
SP  - 634
EP  - 640
DO  - 10.2298/SARH171211030J
ER  - 

@article{
author = "Janković, Ognjenka and Paraš, Smiljana and Tadić-Latinović, Ljiljana and Josipović, Renata and Jokanović, Vukoman and Živković, Slavoljub",
year = "2018",
abstract = "Introduction/Objective The aim of this paper was to verify the biocompatibility of the newly synthesized nanostructured material based on calcium aluminate after implantation into the subcutaneous tissue of rats. Methods The study included 18 rats aged 10-11 weeks, divided into two experimental groups (n = 9). In all animals, incision took place on the back and two pockets of 15 mm in depth were made, in which sterile polyethylene tubes with test materials [calcium aluminate cement (ALBO-CA), calcium silicate cement with the addition of hydroxyapatite (ALBO-CSHA), and mineral trioxide aggregate (MTA) for the control group) were implanted. Six rats of each group were sacrificed in three observational periods (seven, 15, 30 days). Pathohistological analysis included inflammation, bleeding, fibrous capsule, and tissue integrity around the implanted material. Results After seven days of treatment, ALBO-CA and ALBO-CSHA showed better tissue response compared to MTA, with a statistically significant difference in inflammation intensity (p = 0.2781). The difference in vascular congestion and thickness of the fibrous capsule after implantation of ALBO-CA material compared to MTA was also statistically significant (p = 0.5567). At the end of the 30-day evaluation period, an identical inflammatory response of connective tissue at the site of implanting ALBO-CA, ALBO-CSHA, and MTA (score of 0.7) was recorded. The formation of thick or moderately thick fibrous capsule was found to be the thickest in ALBO-CA (grade 3.7). There were no statistically significant differences between the parameters analyzed after 30 days. Conclusion Newly synthesized ALBO-CA showed a satisfactory tissue response and confirmed biocompatibility after implantation in subcutaneous tissue of rats., Uvod/Cilj Cilj ovog rada je bio da se proveri biokompatibilnost novosintetisanog nanostrukturnog materijala na bazi kalcijum-aluminata posle implantacije u potkožno tkivo pacova. Metode U istraživanje je uključeno 18 pacova starosti 10-11 sedmica, koji su podeljeni u dve eksperimentalne grupe (n = 9). Kod svih životinja je urađena incizija na leđima i formirana su dva džepa dubine 15 mm u koja su aplicirane sterilne polietilenske tubice sa testnim materijalima (ALBO-CA, ALBO-CSHA i kontrola MTA). Po šest pacova svake grupe je žrtvovano u tri opservaciona perioda (7, 15 i 30 dana). Patohistološki su analizirani inflamacija, krvarenje, fibrozna kapsula i integritet tkiva oko implantiranog materijala. Rezultati Posle sedam dana ALBO-CA i ALBO-CSHA su pokazali bolji tkivni odgovor u odnosu na MTA sa statistički značajnom razlikom u intenzitetu inflamacije (p = 0,2781). Razlika u vaskularnoj kongestiji i debljini fibrozne kapsule posle implantacije materijala ALBO-CA u odnosu na MTA je takođe bila statistički značajna (p = 0,5567). Na kraju evaluacionog perioda od 30 dana zabeležen je identičan inflamatorni odgovor rastresitog vezivnog tkiva na mestu implantacije ALBO-CA, ALBO-CSHA i MTA (ocena 0,7). Konstatovano je formiranje debele ili umereno debele fibrozne kapsule, koja je bila najdeblja kod ALBO-CA (ocena 3,7). Statistički značajne razlike između analiziranih parametara posle 30 dana nije bilo. Zaključak Novosintetisani ALBO-CA je pokazao zadovoljavajuću tkivnu reakciju i potvrdio biokompatibilnost posle implantacije u potkožno tkivo pacova.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Biocompatibility of nanostructured biomaterials based on calcium aluminate, Biokompatibilnost nanostrukturnih biomaterijala na bazi kalcijum-aluminata",
volume = "146",
number = "11-12",
pages = "634-640",
doi = "10.2298/SARH171211030J"
}

Janković, O., Paraš, S., Tadić-Latinović, L., Josipović, R., Jokanović, V.,& Živković, S.. (2018). Biocompatibility of nanostructured biomaterials based on calcium aluminate. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 146(11-12), 634-640.
https://doi.org/10.2298/SARH171211030J

Janković O, Paraš S, Tadić-Latinović L, Josipović R, Jokanović V, Živković S. Biocompatibility of nanostructured biomaterials based on calcium aluminate. in Srpski arhiv za celokupno lekarstvo. 2018;146(11-12):634-640.
doi:10.2298/SARH171211030J .

Janković, Ognjenka, Paraš, Smiljana, Tadić-Latinović, Ljiljana, Josipović, Renata, Jokanović, Vukoman, Živković, Slavoljub, "Biocompatibility of nanostructured biomaterials based on calcium aluminate" in Srpski arhiv za celokupno lekarstvo, 146, no. 11-12 (2018):634-640,
https://doi.org/10.2298/SARH171211030J . .