TY  - JOUR
AU  - Popović, Jelena
AU  - Cvetković, Tatjana
AU  - Džopalić, Tanja
AU  - Nikolić, Marija
AU  - Mitić, Aleksandar
AU  - Barac, Radomir
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2366
AB  - Introduction Cytokine network plays an important role in pathogenesis of chronic periapical lesions. The aim of this study was to determine the concentration of interleukin-6 (IL-6) in tissue homogenates of human periapical lesions and correlate its levels with symptomatology and size of the lesions. Materials and Methods 93 samples of chronic periapical lesions were obtained after extraction of teeth. Samples were divided according to the clinical presentation as symptomatic and asymptomatic, and according to the size as large and small. The concentration of IL-6 was analyzed using ELISA. Results Statistically significant difference in IL-6 concentration was observed in symptomatic lesions compared to asymptomatic (p lt 0.001). Analysis showed statistically higher concentration in large symptomatic lesions compared to large asymptomatic lesions (p lt 0.001), and in small symptomatic lesions compared to small asymptomatic (p lt 0.05). Higher production of IL-6 was observed in large lesions compared to small but this difference was not statistically significant. Conclusion Higher concentration of IL-6 in lesions with expressed clinical symptoms as well as in large lesions indicates that IL-6 is an important factor responsible for the progression of lesions and bone resorption.
AB  - Uvod Mreža citokina igra značajnu ulogu u patogenezi hroničnih periapeksnih lezija. Cilj studije je bio da se odredi koncentracija IL-6 u tkivnim homogenatima hroničnih periapeksnih lezija i da se proveri korelacija sa simptomatologijom i veličinom lezija. Materijal i metode Ispitana su 93 uzorka hroničnih periapeksnih lezija dobijenih nakon ekstrakcije zuba. Uzorci lezija su podeljeni prema simptomatologiji pacijenata na simptomatske i asimptomatske, a prema veličini na velike i male. Koncentracija IL-6 je ispitivana pomoću ELISA testa, a dobijene vrednosti su analizirane u odnosu na grupe. Rezultati Statistički značajna razlika u koncentraciji IL-6 je uočena u grupi simptomatskih lezija u poređenju sa asimptomatskim (p  lt  0,001). Analiza je pokazala i statistički značajno veću koncentraciju u velikim simptomatskim lezijama u odnosu na velike asimptomatske (p  lt  0,001) i u malim simptomatskim lezijama u odnosu na male asimptomatske (p  lt  0,05). Zapažena je i povećana produkciju IL-6 u velikim lezijama u odnosu na male, međutim, razlika nije bila statistički značajna. Zaključak Veća koncentracija IL-6 u lezijama sa izraženim kliničkim simptomima kao i velikim lezijama ukazuje na njegovu važnu ulogu u progresiji periapeksnih lezija i koštane resorpcije. .
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - The role of interleukin-6 in pathogenesis of chronic periapical lesions
T1  - Uloga interleukina-6 u patogenezi hroničnih periapeksnih lezija
VL  - 65
IS  - 3
SP  - 126
EP  - 132
DO  - 10.2478/sdj-2018-0012
ER  - 

@article{
author = "Popović, Jelena and Cvetković, Tatjana and Džopalić, Tanja and Nikolić, Marija and Mitić, Aleksandar and Barac, Radomir and Živković, Slavoljub",
year = "2018",
abstract = "Introduction Cytokine network plays an important role in pathogenesis of chronic periapical lesions. The aim of this study was to determine the concentration of interleukin-6 (IL-6) in tissue homogenates of human periapical lesions and correlate its levels with symptomatology and size of the lesions. Materials and Methods 93 samples of chronic periapical lesions were obtained after extraction of teeth. Samples were divided according to the clinical presentation as symptomatic and asymptomatic, and according to the size as large and small. The concentration of IL-6 was analyzed using ELISA. Results Statistically significant difference in IL-6 concentration was observed in symptomatic lesions compared to asymptomatic (p lt 0.001). Analysis showed statistically higher concentration in large symptomatic lesions compared to large asymptomatic lesions (p lt 0.001), and in small symptomatic lesions compared to small asymptomatic (p lt 0.05). Higher production of IL-6 was observed in large lesions compared to small but this difference was not statistically significant. Conclusion Higher concentration of IL-6 in lesions with expressed clinical symptoms as well as in large lesions indicates that IL-6 is an important factor responsible for the progression of lesions and bone resorption., Uvod Mreža citokina igra značajnu ulogu u patogenezi hroničnih periapeksnih lezija. Cilj studije je bio da se odredi koncentracija IL-6 u tkivnim homogenatima hroničnih periapeksnih lezija i da se proveri korelacija sa simptomatologijom i veličinom lezija. Materijal i metode Ispitana su 93 uzorka hroničnih periapeksnih lezija dobijenih nakon ekstrakcije zuba. Uzorci lezija su podeljeni prema simptomatologiji pacijenata na simptomatske i asimptomatske, a prema veličini na velike i male. Koncentracija IL-6 je ispitivana pomoću ELISA testa, a dobijene vrednosti su analizirane u odnosu na grupe. Rezultati Statistički značajna razlika u koncentraciji IL-6 je uočena u grupi simptomatskih lezija u poređenju sa asimptomatskim (p  lt  0,001). Analiza je pokazala i statistički značajno veću koncentraciju u velikim simptomatskim lezijama u odnosu na velike asimptomatske (p  lt  0,001) i u malim simptomatskim lezijama u odnosu na male asimptomatske (p  lt  0,05). Zapažena je i povećana produkciju IL-6 u velikim lezijama u odnosu na male, međutim, razlika nije bila statistički značajna. Zaključak Veća koncentracija IL-6 u lezijama sa izraženim kliničkim simptomima kao i velikim lezijama ukazuje na njegovu važnu ulogu u progresiji periapeksnih lezija i koštane resorpcije. .",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "The role of interleukin-6 in pathogenesis of chronic periapical lesions, Uloga interleukina-6 u patogenezi hroničnih periapeksnih lezija",
volume = "65",
number = "3",
pages = "126-132",
doi = "10.2478/sdj-2018-0012"
}

Popović, J., Cvetković, T., Džopalić, T., Nikolić, M., Mitić, A., Barac, R.,& Živković, S.. (2018). The role of interleukin-6 in pathogenesis of chronic periapical lesions. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(3), 126-132.
https://doi.org/10.2478/sdj-2018-0012

Popović J, Cvetković T, Džopalić T, Nikolić M, Mitić A, Barac R, Živković S. The role of interleukin-6 in pathogenesis of chronic periapical lesions. in Stomatološki glasnik Srbije. 2018;65(3):126-132.
doi:10.2478/sdj-2018-0012 .

Popović, Jelena, Cvetković, Tatjana, Džopalić, Tanja, Nikolić, Marija, Mitić, Aleksandar, Barac, Radomir, Živković, Slavoljub, "The role of interleukin-6 in pathogenesis of chronic periapical lesions" in Stomatološki glasnik Srbije, 65, no. 3 (2018):126-132,
https://doi.org/10.2478/sdj-2018-0012 . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Mitić, Vladimir
AU  - Nikolić, Marija
AU  - Dačić, Stefan
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2153
AB  - Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur.
AB  - Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta medica Medianae
T1  - Effects of laser treatment on intracanal dentine and cementum at the root apex
T1  - Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba
VL  - 55
IS  - 1
SP  - 26
EP  - 32
DO  - 10.5633/amm.2016.0104
ER  - 

@article{
author = "Mitić, Aleksandar and Popović, Jelena and Živković, Slavoljub and Mitić, Vladimir and Nikolić, Marija and Dačić, Stefan",
year = "2016",
abstract = "Laser irradiation during root canal treatment is efficient but in order to prevent irreversible thermal damage to dentine, cementum and other periapical structures continuous water cooling is required. The aim of this study was to ultrastructurally analyze the surface of intracanal dentine and cementum at the root apex after Er:YAG laser irradiation with and without sterile saline solution irrigation. The study involved 70 intact maxillary and mandibular single-rooted and tworooted human teeth, divided into two experimental groups and one control group. In all teeth access cavity was created. For root canal irradiation, Er:YAG laser with the wavelength of 2940 nm, energy output of 140 mJ per pulse, pulse duration of 125-160 ms, pulse repetition rate of 10 Hz and power of 15 W was used. Total irradiation time for each root canal was 10 seconds. The ultrastructural analysis of intracanal dentine and the cementum of the root apex was done by using scanning electron microscope (JEOL JSM-5300 Tokyo Japan). Intracanal Er:YAG laser irradiation without water cooling showed melted circumpulpal dentine with large cracks in it, whereas cementum at the root apex was thermally modified in the form of plates and cracks. Intracanal Er:YAG laser irradiation with continuous water cooling resulted in clean intracanal dentine surface without morphological changes of the cementum. The canal system irradiation is acceptable and possible only when using constant water cooling; otherwise, irreversible thermal damage to intracanal dentine and cementum at the root apex occur., Obrada kanala korena zuba laserom je efikasna, ali zahteva obavezno hlađenje vodom kako bi se sprečila ireverzibilna termička oštećenja dentina, cementa i ostalih periapeksnih struktura. Cilj ovog rada bio je da se ultrastrukturno analizira površina intrakanalnog dentina i cementnog tkiva na apeksu korena zuba posle obrade kanala Er:YAG laserom bez irigacije i sa irigacijom sterilnim fiziološkim rastvorom. Korišćeno je 70 intaktnih maksilarnih i mandibularnih jednokorenih i dvokorenih humanih zuba, podeljenih u dve eksperimentalne i jednu kontrolnu grupu. Kod svih zuba je formiran pristupni kavitet. Za obradu kanala korišćen je Er:YAG laser talasne dužine 2940 nm, izlazne energije za impuls 140 mJ; u pulsnom režimu 125-160ms, stopom pulsne repeticije od 10 Hz i energijom od 15 W. Vreme obrade svakog kanala laserom iznosilo je 10 sekundi. Ultrastrukturna analiza intrakanalnog dentina i cementnog tkiva na apeksnom delu korena zuba urađena je na skening elektronskom mikroskopu (JEOL JSM -5300 Tokyo Japan). Intrakanalna obrada Er:YAG laserom bez vodenog hlađenja pokazuje stopljen cirkumpulparni dentin sa velikim pukotinama u dentinu, a cementno tkivo na apeksu je termički modifikovano u vidu ploča i pukotina. Intrakanalna obrada Er:YAG laserom uz kontinuirano vodeno hlađenje daje čistu intrakanalnu dentinsku površinu bez morfoloških promena cementnog tkiva. Obrada kanalnog sistema zuba je opravdana i moguća samo u uslovima stalnog hlađenja vodom, u protivnom nastaju ireverzibilna termička oštećenja intrakanalnog dentina i cementa na apeksu korena zuba.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta medica Medianae",
title = "Effects of laser treatment on intracanal dentine and cementum at the root apex, Efekti lasero-terapije na intrakanalni dentin i cementno tkivo apeksa korena zuba",
volume = "55",
number = "1",
pages = "26-32",
doi = "10.5633/amm.2016.0104"
}

Mitić, A., Popović, J., Živković, S., Mitić, V., Nikolić, M.,& Dačić, S.. (2016). Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae
Univerzitet u Nišu - Medicinski fakultet, Niš., 55(1), 26-32.
https://doi.org/10.5633/amm.2016.0104

Mitić A, Popović J, Živković S, Mitić V, Nikolić M, Dačić S. Effects of laser treatment on intracanal dentine and cementum at the root apex. in Acta medica Medianae. 2016;55(1):26-32.
doi:10.5633/amm.2016.0104 .

Mitić, Aleksandar, Popović, Jelena, Živković, Slavoljub, Mitić, Vladimir, Nikolić, Marija, Dačić, Stefan, "Effects of laser treatment on intracanal dentine and cementum at the root apex" in Acta medica Medianae, 55, no. 1 (2016):26-32,
https://doi.org/10.5633/amm.2016.0104 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Radenković, Goran
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2144
AB  - The application of irrigating solutions is essential in chemomechanical treatment of tooth root canal. However, chemical and electrochemical aggressiveness of the solutions, which directly act on the instruments, may damage their surface. The aim of this study was to investigate the sensitivity of the nickel-titanium (Ni-Ti) and stainless steel endodontic files to corrosive action of the sodium hypochlorite (NaOCl), chlorhexidine gluconate (CHX) and ethylene-diamine tetraacetic acid (EDTA). Testing of sensitivity to corrosion of the instruments was performed by dynamic potentiometric method. Measurements were made in 5.25% NaOCl, 0.2% CHX and 17% EDTA. Ni-Ti instruments immersed in 5.25% NaOCl showed the most intensive corrosive changes and the lowest value of pitting potential of 1.1 V. Stainless steel instruments immersed in 5.25% NaOCl showed higher value of pitting potential of 1.5 V. Stainless steel instruments immersed in 0.2% CHX showed lower corrosive surface changes and higher value of pitting potential of 1.6 V, whereas Ni-Ti instruments immersed in 0.2% CHX showed the pitting potential of 1.9 V. The corrosion was not observed in both types of instruments after immersion in 17% EDTA. The use of 5.25% NaOCl and 0.2% CHX may cause severe surface corrosion of Ni-Ti and stainless steel endodontic files.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly / CICEQ
T1  - The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions
VL  - 22
IS  - 1
SP  - 95
EP  - 100
DO  - 10.2298/CICEQ150103023P
ER  - 

@article{
author = "Popović, Jelena and Radenković, Goran and Gašić, Jovanka and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir",
year = "2016",
abstract = "The application of irrigating solutions is essential in chemomechanical treatment of tooth root canal. However, chemical and electrochemical aggressiveness of the solutions, which directly act on the instruments, may damage their surface. The aim of this study was to investigate the sensitivity of the nickel-titanium (Ni-Ti) and stainless steel endodontic files to corrosive action of the sodium hypochlorite (NaOCl), chlorhexidine gluconate (CHX) and ethylene-diamine tetraacetic acid (EDTA). Testing of sensitivity to corrosion of the instruments was performed by dynamic potentiometric method. Measurements were made in 5.25% NaOCl, 0.2% CHX and 17% EDTA. Ni-Ti instruments immersed in 5.25% NaOCl showed the most intensive corrosive changes and the lowest value of pitting potential of 1.1 V. Stainless steel instruments immersed in 5.25% NaOCl showed higher value of pitting potential of 1.5 V. Stainless steel instruments immersed in 0.2% CHX showed lower corrosive surface changes and higher value of pitting potential of 1.6 V, whereas Ni-Ti instruments immersed in 0.2% CHX showed the pitting potential of 1.9 V. The corrosion was not observed in both types of instruments after immersion in 17% EDTA. The use of 5.25% NaOCl and 0.2% CHX may cause severe surface corrosion of Ni-Ti and stainless steel endodontic files.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly / CICEQ",
title = "The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions",
volume = "22",
number = "1",
pages = "95-100",
doi = "10.2298/CICEQ150103023P"
}

Popović, J., Radenković, G., Gašić, J., Živković, S., Mitić, A., Nikolić, M.,& Barac, R.. (2016). The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions. in Chemical Industry & Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 22(1), 95-100.
https://doi.org/10.2298/CICEQ150103023P

Popović J, Radenković G, Gašić J, Živković S, Mitić A, Nikolić M, Barac R. The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions. in Chemical Industry & Chemical Engineering Quarterly / CICEQ. 2016;22(1):95-100.
doi:10.2298/CICEQ150103023P .

Popović, Jelena, Radenković, Goran, Gašić, Jovanka, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, "The examination of sensitivity to corrosion of nickel-titanium and stainless steel endodontic instruments in tooth root canal irrigating solutions" in Chemical Industry & Chemical Engineering Quarterly / CICEQ, 22, no. 1 (2016):95-100,
https://doi.org/10.2298/CICEQ150103023P . .

TY  - JOUR
AU  - Barac, Radomir
AU  - Popović, Jelena
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Gašić, Jovanka
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2109
AB  - Introduction During endodontic treatment smaller or larger areas of root canal wall remain non-instrumented. This can affect prognosis of endodontic treatment as some bacteria may be left behind. The purpose of this study was to evaluate the morphology of non-instrumented areas of the root canal wall using scanning-electron-microscopy (SEM) after completed instrumentation and various irrigation regiments. Materials and Methods Eighteen single-rooted extracted teeth were divided into the six groups. One tooth in each group represented a control sample. In all samples only one half of the canal was instrumented using ISO 40 hand files. Control samples were subjected to an irrigation protocols without instrumentation. Irrigants used were physiological saline, 3% sodium hypochlorite and 15% of ethylene-diamine-tetra-acetate. Irrigation protocol included using each of these irrigants alone, or a combination of NaOCl and EDTA, as well as their combination with final irrigation using NaOCl or chlorhexidine. Then after, roots were sectioned longitudinally and prepared for SEM. Results Saline irrigation left pulpal debris on uninstrumented areas of the canal wall. Irrigation with 3% NaOCl left behind canal wall with different forms of calcospherites. However, after EDTA irrigation dentin appeared as an undulating surface with open tubules without a smear layer. The combination of NaOCl and EDTA showed remnants of calcospherites and open slightly widened dentinal tubules. Final irrigation with NaOCl on the uninstrumented areas showed enlarged dentinal tubules along with dentinal erosion, while after final irrigation with CHX clean dentin and open dentinal tubules without smear layer were noted. Conclusion From the morphological point of view, the most favorable effect of irrigation on both uninstrumented and uninstrumented canal walls was achieved after irrigation with NaOCl and EDTA or NaOCl, EDTA and chlorhexidine as the final irrigant.
AB  - Uvod Tokom endodontske terapije manje ili veće površine kanala korena zuba ostaju neinstrumentisane i mogu sadržavati bakterije, što može dovesti do neuspešnog ishoda. Cilj rada je bio da se skening-elekron-mikroskopskom (SEM) analizom proceni morfologija neistrumentisanih delova zidova kanala posle instrumentacije i primene različitih irigacionih protokola. Materijal i metode rada Osamnaest jednokorenih ekstrahovanih zuba je podeljeno u šest grupa. Jedan zub iz svake grupe je predstavljao kontrolni uzorak. Eksperimentalni uzorci su preparisani do instrumenta veličine ISO40. Kod svakog zuba je instrumentisana samo jedna polovina kanala. Kontrolni uzorci su podvrgnuti samo irigacionim protokolima bez preparacije. Osim fiziološkog rastvora, 3% natrijum-hipohlorita i 15% etilen-diamin-tetra-acetata, koji su primenjeni samostalno, eksperimentalni uzorci su irigirani i sa kombinacijom NaOCl i EDTA, kao i sa kombinacijom istih iriganasa, pri čemu je kao završni irigans korišćen ili NaOCl ili hlorheksidin. Korenovi su uzdužno presečeni i pripremljeni za SEM. Rezultati Posle irigacije sa fiziološkim rastvorom u neinstrumentisanim delovima kanala uočen je dentin pokriven debrisom, posle irigacije sa NaOCl dentin sa različitim oblicima kalcisferita. Posle irigacije sa EDTA uočena je talasasta površina dentinskog zida sa otvorenim tubulima bez razmaznog sloja. Irigacija kombinacijom NaOCl i EDTA je pokazala ostatke kalcisferita i otvorene, blago proširene dentinske tubule. Završno ispiranje sa NaOCl na neinstrumentisanim delovima dentina pokazuje proširene dentinske tubule i eroziju dentina, a završno ispiranje sa CHX čist dentin i otvorene dentinske tubule bez razmaznog sloja. Zaključak Sa morfološkog aspekta, najpovoljniji efekat irigacije (i kod instrumentisanih i kod neinstrumentisanih delova kanala) ustanovljen je posle irigacionih protokola sa: NaOCl i EDTA i NaOCl, EDTA i hlorheksidinom kao završnim irigansom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens
T1  - Ultrastrukturna analiza neinstrumentisanih površina u kanalu korena posle različitih irigacionih protokola
VL  - 63
IS  - 2
SP  - 57
EP  - 65
DO  - 10.1515/sdj-2016-0006
ER  - 

@article{
author = "Barac, Radomir and Popović, Jelena and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Gašić, Jovanka",
year = "2016",
abstract = "Introduction During endodontic treatment smaller or larger areas of root canal wall remain non-instrumented. This can affect prognosis of endodontic treatment as some bacteria may be left behind. The purpose of this study was to evaluate the morphology of non-instrumented areas of the root canal wall using scanning-electron-microscopy (SEM) after completed instrumentation and various irrigation regiments. Materials and Methods Eighteen single-rooted extracted teeth were divided into the six groups. One tooth in each group represented a control sample. In all samples only one half of the canal was instrumented using ISO 40 hand files. Control samples were subjected to an irrigation protocols without instrumentation. Irrigants used were physiological saline, 3% sodium hypochlorite and 15% of ethylene-diamine-tetra-acetate. Irrigation protocol included using each of these irrigants alone, or a combination of NaOCl and EDTA, as well as their combination with final irrigation using NaOCl or chlorhexidine. Then after, roots were sectioned longitudinally and prepared for SEM. Results Saline irrigation left pulpal debris on uninstrumented areas of the canal wall. Irrigation with 3% NaOCl left behind canal wall with different forms of calcospherites. However, after EDTA irrigation dentin appeared as an undulating surface with open tubules without a smear layer. The combination of NaOCl and EDTA showed remnants of calcospherites and open slightly widened dentinal tubules. Final irrigation with NaOCl on the uninstrumented areas showed enlarged dentinal tubules along with dentinal erosion, while after final irrigation with CHX clean dentin and open dentinal tubules without smear layer were noted. Conclusion From the morphological point of view, the most favorable effect of irrigation on both uninstrumented and uninstrumented canal walls was achieved after irrigation with NaOCl and EDTA or NaOCl, EDTA and chlorhexidine as the final irrigant., Uvod Tokom endodontske terapije manje ili veće površine kanala korena zuba ostaju neinstrumentisane i mogu sadržavati bakterije, što može dovesti do neuspešnog ishoda. Cilj rada je bio da se skening-elekron-mikroskopskom (SEM) analizom proceni morfologija neistrumentisanih delova zidova kanala posle instrumentacije i primene različitih irigacionih protokola. Materijal i metode rada Osamnaest jednokorenih ekstrahovanih zuba je podeljeno u šest grupa. Jedan zub iz svake grupe je predstavljao kontrolni uzorak. Eksperimentalni uzorci su preparisani do instrumenta veličine ISO40. Kod svakog zuba je instrumentisana samo jedna polovina kanala. Kontrolni uzorci su podvrgnuti samo irigacionim protokolima bez preparacije. Osim fiziološkog rastvora, 3% natrijum-hipohlorita i 15% etilen-diamin-tetra-acetata, koji su primenjeni samostalno, eksperimentalni uzorci su irigirani i sa kombinacijom NaOCl i EDTA, kao i sa kombinacijom istih iriganasa, pri čemu je kao završni irigans korišćen ili NaOCl ili hlorheksidin. Korenovi su uzdužno presečeni i pripremljeni za SEM. Rezultati Posle irigacije sa fiziološkim rastvorom u neinstrumentisanim delovima kanala uočen je dentin pokriven debrisom, posle irigacije sa NaOCl dentin sa različitim oblicima kalcisferita. Posle irigacije sa EDTA uočena je talasasta površina dentinskog zida sa otvorenim tubulima bez razmaznog sloja. Irigacija kombinacijom NaOCl i EDTA je pokazala ostatke kalcisferita i otvorene, blago proširene dentinske tubule. Završno ispiranje sa NaOCl na neinstrumentisanim delovima dentina pokazuje proširene dentinske tubule i eroziju dentina, a završno ispiranje sa CHX čist dentin i otvorene dentinske tubule bez razmaznog sloja. Zaključak Sa morfološkog aspekta, najpovoljniji efekat irigacije (i kod instrumentisanih i kod neinstrumentisanih delova kanala) ustanovljen je posle irigacionih protokola sa: NaOCl i EDTA i NaOCl, EDTA i hlorheksidinom kao završnim irigansom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens, Ultrastrukturna analiza neinstrumentisanih površina u kanalu korena posle različitih irigacionih protokola",
volume = "63",
number = "2",
pages = "57-65",
doi = "10.1515/sdj-2016-0006"
}

Barac, R., Popović, J., Živković, S., Mitić, A., Nikolić, M.,& Gašić, J.. (2016). Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(2), 57-65.
https://doi.org/10.1515/sdj-2016-0006

Barac R, Popović J, Živković S, Mitić A, Nikolić M, Gašić J. Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens. in Stomatološki glasnik Srbije. 2016;63(2):57-65.
doi:10.1515/sdj-2016-0006 .

Barac, Radomir, Popović, Jelena, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Gašić, Jovanka, "Ultrastructural analysis of uninstrumented root canal areas following various irrigation regimens" in Stomatološki glasnik Srbije, 63, no. 2 (2016):57-65,
https://doi.org/10.1515/sdj-2016-0006 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Radenković, Goran
AU  - Gašić, Jovanka
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
AU  - Živković, Slavoljub
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2026
AB  - Introduction Separation (fracture) of endodontic instruments in the root canal during chemomechanical instrumentation is a complication that can compromise the final outcome of endodontic treatment. One of the most common factors that cause fatigue of endodontic instruments and consequent fracture is surface corrosion. The aim of this study was to investigate the ultrastructure of surface corrosion of endodontic instruments made of stainless steel and nickel-titanium after immersion in the most commonly used root canal irrigants. Material and Methods The study included 48 nickel-titanium and stainless steel endodontic hand files. All instruments were immersed in 5.25% sodium hypochlorite, 0.2% CHX and 17% EDTA. Surface corrosion was analyzed using a scanning electron microscope (SEM). Results Nickel-titanium instruments showed significantly higher susceptibility to corrosion after immersion in 5.25% sodium hypochlorite compared to stainless steel instruments (p lt 0,001). After immersion in 0.2% CHX corrosion damage was observed on both nickel-titanium and stainless steel instruments but the difference was not statistically significant (p=0.096). No corrosion was observed in both types of instruments after immersion in 17% EDTA. Conclusion The use of 5.25% NaOCl and 0.2% CHX as root canal irrigating solutions can cause serious corrosion changes on the surface of nickel-titanium and stainless steel endodontic instruments.
AB  - Uvod Fraktura endodontskih instrumenata u kanalu korena tokom hemomehaničke preparacije je značajna komplikacija koja može ugroziti konačan ishod endodontskog lečenja. Jedan od važnih faktora koji izazivaju zamor materijala endodontskog instrumenta je površinska korozija. Cilj ovog rada je bio da se ispita ultrastruktura površine endodontskih instrumenata od nerđajućeg čelika i nikl-titanijuma nakon potapanja u najčešće upotrebljavane rastvore za irigaciju kanala korena zuba. Materijal i metode rada U istraživanju je korišćeno 48 instrumenata od nikl-titanijuma i nerđajućeg čelika. Testiranje osetljivosti na koroziju je izvršeno potapanjem u rastvore NaOCl od 5,25%, CHX od 0,2% i EDTA od 17%. Analiza korozivnih oštećenja je urađena pomoću skening elektronskog mikroskopa (SEM) na različitim uveličanjima. Rezultati Instrumenti od nikl-titanijuma su pokazali statistički značajno veću osetljivost na koroziju nakon potapanja u 5,25% NaOCl u poređenju sa instrumentima od nerđajućeg čelika (p lt 0,001). Nakon potapanja u 0,2% CHX uočeno je korozivno oštećenje instrumenata od nikl-titanijuma i nerđajućeg čelika, ali razlika nije bila statistički značajna. Korozija nakon potapanja u 17% EDTA nije uočena ni kod jedne vrste endodontskih instrumenata. Zaključak Primena 5,25% NaOCl i 0,2% CHX kao rastvora za irigaciju kanala korena može izazvati ozbiljne korozivne promene na površini instrumenata od nikl-titanijuma i nerđajućeg čelika.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions
T1  - Ultrastrukturna analiza površine endodontskih instrumenata nakon potapanja u rastvore za irigaciju
VL  - 62
IS  - 4
SP  - 157
EP  - 165
DO  - 10.1515/sdj-2015-0016
ER  - 

@article{
author = "Popović, Jelena and Radenković, Goran and Gašić, Jovanka and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir and Živković, Slavoljub",
year = "2015",
abstract = "Introduction Separation (fracture) of endodontic instruments in the root canal during chemomechanical instrumentation is a complication that can compromise the final outcome of endodontic treatment. One of the most common factors that cause fatigue of endodontic instruments and consequent fracture is surface corrosion. The aim of this study was to investigate the ultrastructure of surface corrosion of endodontic instruments made of stainless steel and nickel-titanium after immersion in the most commonly used root canal irrigants. Material and Methods The study included 48 nickel-titanium and stainless steel endodontic hand files. All instruments were immersed in 5.25% sodium hypochlorite, 0.2% CHX and 17% EDTA. Surface corrosion was analyzed using a scanning electron microscope (SEM). Results Nickel-titanium instruments showed significantly higher susceptibility to corrosion after immersion in 5.25% sodium hypochlorite compared to stainless steel instruments (p lt 0,001). After immersion in 0.2% CHX corrosion damage was observed on both nickel-titanium and stainless steel instruments but the difference was not statistically significant (p=0.096). No corrosion was observed in both types of instruments after immersion in 17% EDTA. Conclusion The use of 5.25% NaOCl and 0.2% CHX as root canal irrigating solutions can cause serious corrosion changes on the surface of nickel-titanium and stainless steel endodontic instruments., Uvod Fraktura endodontskih instrumenata u kanalu korena tokom hemomehaničke preparacije je značajna komplikacija koja može ugroziti konačan ishod endodontskog lečenja. Jedan od važnih faktora koji izazivaju zamor materijala endodontskog instrumenta je površinska korozija. Cilj ovog rada je bio da se ispita ultrastruktura površine endodontskih instrumenata od nerđajućeg čelika i nikl-titanijuma nakon potapanja u najčešće upotrebljavane rastvore za irigaciju kanala korena zuba. Materijal i metode rada U istraživanju je korišćeno 48 instrumenata od nikl-titanijuma i nerđajućeg čelika. Testiranje osetljivosti na koroziju je izvršeno potapanjem u rastvore NaOCl od 5,25%, CHX od 0,2% i EDTA od 17%. Analiza korozivnih oštećenja je urađena pomoću skening elektronskog mikroskopa (SEM) na različitim uveličanjima. Rezultati Instrumenti od nikl-titanijuma su pokazali statistički značajno veću osetljivost na koroziju nakon potapanja u 5,25% NaOCl u poređenju sa instrumentima od nerđajućeg čelika (p lt 0,001). Nakon potapanja u 0,2% CHX uočeno je korozivno oštećenje instrumenata od nikl-titanijuma i nerđajućeg čelika, ali razlika nije bila statistički značajna. Korozija nakon potapanja u 17% EDTA nije uočena ni kod jedne vrste endodontskih instrumenata. Zaključak Primena 5,25% NaOCl i 0,2% CHX kao rastvora za irigaciju kanala korena može izazvati ozbiljne korozivne promene na površini instrumenata od nikl-titanijuma i nerđajućeg čelika.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions, Ultrastrukturna analiza površine endodontskih instrumenata nakon potapanja u rastvore za irigaciju",
volume = "62",
number = "4",
pages = "157-165",
doi = "10.1515/sdj-2015-0016"
}

Popović, J., Radenković, G., Gašić, J., Mitić, A., Nikolić, M., Barac, R.,& Živković, S.. (2015). Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(4), 157-165.
https://doi.org/10.1515/sdj-2015-0016

Popović J, Radenković G, Gašić J, Mitić A, Nikolić M, Barac R, Živković S. Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions. in Stomatološki glasnik Srbije. 2015;62(4):157-165.
doi:10.1515/sdj-2015-0016 .

Popović, Jelena, Radenković, Goran, Gašić, Jovanka, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, Živković, Slavoljub, "Ultrastructural analysis of the surface of endodontic instruments after immersion in irrigating solutions" in Stomatološki glasnik Srbije, 62, no. 4 (2015):157-165,
https://doi.org/10.1515/sdj-2015-0016 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Kesić, Ljiljana
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2007
AB  - Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion. Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p  lt  0.001). Large symptomatic lesions were 73.50 times more likely to harbor HCMV than small symptomatic lesions (p  lt  0.001). Large symptomatic lesions were 7.64 times more likely to be infected with EBV than small symptomatic lesions (p = 0.05). Large symptomatic lesions were 5.38 times more likely to harbor dual HCMV/EBV infection than small symptomatic lesions (p = 0.115). Conclusion: Detection of HCMV and EBV in the samples of periapical lesions suggests an important role of herpesviruses in periapical tissue destruction.
PB  - Karger, Basel
T2  - Intervirology
T1  - Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions
VL  - 58
IS  - 5
SP  - 271
EP  - 277
DO  - 10.1159/000441208
ER  - 

@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Kesić, Ljiljana and Mitić, Aleksandar and Nikolić, Marija and Milašin, Jelena",
year = "2015",
abstract = "Objective: The aim of this study was to investigate the presence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) in the tissue of chronic periapical lesions, and to compare the results in relation to the symptoms of patients and the size of the lesion. Methods: Periapical lesions analyzed in the study were collected from the roots of the teeth indicated for extraction. Samples were divided according to the symptoms into groups of symptomatic and asymptomatic, and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p  lt  0.001). Large symptomatic lesions were 73.50 times more likely to harbor HCMV than small symptomatic lesions (p  lt  0.001). Large symptomatic lesions were 7.64 times more likely to be infected with EBV than small symptomatic lesions (p = 0.05). Large symptomatic lesions were 5.38 times more likely to harbor dual HCMV/EBV infection than small symptomatic lesions (p = 0.115). Conclusion: Detection of HCMV and EBV in the samples of periapical lesions suggests an important role of herpesviruses in periapical tissue destruction.",
publisher = "Karger, Basel",
journal = "Intervirology",
title = "Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions",
volume = "58",
number = "5",
pages = "271-277",
doi = "10.1159/000441208"
}

Popović, J., Gašić, J., Živković, S., Kesić, L., Mitić, A., Nikolić, M.,& Milašin, J.. (2015). Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions. in Intervirology
Karger, Basel., 58(5), 271-277.
https://doi.org/10.1159/000441208

Popović J, Gašić J, Živković S, Kesić L, Mitić A, Nikolić M, Milašin J. Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions. in Intervirology. 2015;58(5):271-277.
doi:10.1159/000441208 .

Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Kesić, Ljiljana, Mitić, Aleksandar, Nikolić, Marija, Milašin, Jelena, "Prevalence of Human Cytomegalovirus and Epstein-Barr Virus in Chronic Periapical Lesions" in Intervirology, 58, no. 5 (2015):271-277,
https://doi.org/10.1159/000441208 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Cvetković, Tatjana
AU  - Džopalić, Tanja
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
AU  - Živković, Slavoljub
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1870
AB  - Introduction The balance between proinflammatory and anti-inflammatory cytokines plays an important role in the pathogenesis of chronic periapical lesions. The aim of this study was to determine the concentration of TNF-α in tissue homogenates of periapical lesions and analyze its levels in relation to the symptomatology and the size of lesions. Materials and Methods 93 samples of chronic periapical lesions were obtained after extraction of teeth. Samples were classified according to the clinical presentation as symptomatic and asymptomatic, and according to the size as large and small. The concentration of TNF-α was analyzed using ELISA. Results The results showed increased production of TNF-α in symptomatic lesions compared to asymptomatic. Higher concentration of TNF-α was demonstrated in large lesions compared to small. Large symptomatic lesions showed greater concentration of TNF-α compared to small symptomatic lesions, while bigger asymptomatic lesions demonstrated higher amount of the cytokines compared to small asymptomatic lesions. Conclusion Higher concentration of TNF-α in large symptomatic lesions indicates that TNF-α is an important factor responsible for the progression of lesions.
AB  - Uvod Balans između proinflamatornih i antiinflamatornih citokina igra važnu ulogu u patogenezi hroničnih periapeksnih lezija. Cilj ove studije je bio da se odredi koncentracija faktora nekroze tumora alfa (TNF-α) u homogenatima tkiva periapeksnih lezija i rezultati analiziraju u pogledu simptomatologije tih lezija kod pacijenata, odnosno veličine lezije. Materijal i metode rada Ispitana su 93 uzorka hroničnih periapeksnih lezija dobijenih nakon ekstrakcije zuba. Uzorci lezija su, prema simptomatologiji pacijenata, podeljeni na simptomatske i asimptomatske, a prema veličini na velike i male. Koncentracija TNF-α u uzorku ispitivana je pomoću testa ELISA. Rezultati Kod klinički simptomatskih lezija uočeno je povećano stvaranje TNF-α u odnosu na asimptomatske. Veća koncentracija TNF-α je dokazana i u velikim lezijama u odnosu na male. Velike simptomatske lezije su pokazale veću koncentraciju TNF-α u odnosu na male simptomatske lezije, dok su velike asimptomatske lezije imale veću količinu ovog citokina u odnosu na male asimptomatske lezije. Zaključak Veća koncentracija TNF-α u velikim i u lezijama s pojačanim kliničkim simptomima pokazuje da je TNF-α važan faktor odgovoran za napredovanje lezije.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - The concentration of tumor necrosis factor alpha in periapical lesions
T1  - Ispitivanje koncentracije faktora nekroze tumora alfa u tkivnim homogenatima periapeksnih lezija
VL  - 61
IS  - 1
SP  - 7
EP  - 13
DO  - 10.2298/sgs1401007p
ER  - 

@article{
author = "Popović, Jelena and Cvetković, Tatjana and Džopalić, Tanja and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir and Živković, Slavoljub",
year = "2014",
abstract = "Introduction The balance between proinflammatory and anti-inflammatory cytokines plays an important role in the pathogenesis of chronic periapical lesions. The aim of this study was to determine the concentration of TNF-α in tissue homogenates of periapical lesions and analyze its levels in relation to the symptomatology and the size of lesions. Materials and Methods 93 samples of chronic periapical lesions were obtained after extraction of teeth. Samples were classified according to the clinical presentation as symptomatic and asymptomatic, and according to the size as large and small. The concentration of TNF-α was analyzed using ELISA. Results The results showed increased production of TNF-α in symptomatic lesions compared to asymptomatic. Higher concentration of TNF-α was demonstrated in large lesions compared to small. Large symptomatic lesions showed greater concentration of TNF-α compared to small symptomatic lesions, while bigger asymptomatic lesions demonstrated higher amount of the cytokines compared to small asymptomatic lesions. Conclusion Higher concentration of TNF-α in large symptomatic lesions indicates that TNF-α is an important factor responsible for the progression of lesions., Uvod Balans između proinflamatornih i antiinflamatornih citokina igra važnu ulogu u patogenezi hroničnih periapeksnih lezija. Cilj ove studije je bio da se odredi koncentracija faktora nekroze tumora alfa (TNF-α) u homogenatima tkiva periapeksnih lezija i rezultati analiziraju u pogledu simptomatologije tih lezija kod pacijenata, odnosno veličine lezije. Materijal i metode rada Ispitana su 93 uzorka hroničnih periapeksnih lezija dobijenih nakon ekstrakcije zuba. Uzorci lezija su, prema simptomatologiji pacijenata, podeljeni na simptomatske i asimptomatske, a prema veličini na velike i male. Koncentracija TNF-α u uzorku ispitivana je pomoću testa ELISA. Rezultati Kod klinički simptomatskih lezija uočeno je povećano stvaranje TNF-α u odnosu na asimptomatske. Veća koncentracija TNF-α je dokazana i u velikim lezijama u odnosu na male. Velike simptomatske lezije su pokazale veću koncentraciju TNF-α u odnosu na male simptomatske lezije, dok su velike asimptomatske lezije imale veću količinu ovog citokina u odnosu na male asimptomatske lezije. Zaključak Veća koncentracija TNF-α u velikim i u lezijama s pojačanim kliničkim simptomima pokazuje da je TNF-α važan faktor odgovoran za napredovanje lezije.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "The concentration of tumor necrosis factor alpha in periapical lesions, Ispitivanje koncentracije faktora nekroze tumora alfa u tkivnim homogenatima periapeksnih lezija",
volume = "61",
number = "1",
pages = "7-13",
doi = "10.2298/sgs1401007p"
}

Popović, J., Cvetković, T., Džopalić, T., Mitić, A., Nikolić, M., Barac, R.,& Živković, S.. (2014). The concentration of tumor necrosis factor alpha in periapical lesions. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 61(1), 7-13.
https://doi.org/10.2298/sgs1401007p

Popović J, Cvetković T, Džopalić T, Mitić A, Nikolić M, Barac R, Živković S. The concentration of tumor necrosis factor alpha in periapical lesions. in Stomatološki glasnik Srbije. 2014;61(1):7-13.
doi:10.2298/sgs1401007p .

Popović, Jelena, Cvetković, Tatjana, Džopalić, Tanja, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, Živković, Slavoljub, "The concentration of tumor necrosis factor alpha in periapical lesions" in Stomatološki glasnik Srbije, 61, no. 1 (2014):7-13,
https://doi.org/10.2298/sgs1401007p . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Mitić, Aleksandar
AU  - Nikolić, Marija
AU  - Barac, Radomir
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1864
AB  - Introduction Although many studies have reported various advantages of nickel-titanium endodontic instruments, the use of stainless steel endodontic files still presents a standard in everyday practice. The aim of this study was to evaluate ultrastructure of the surface of stainless steel endodontic instruments before and after their use. Material and Methods Fourteen sets of non-used stainless steel endodontic reamers and files were included in the study. Each set of instruments was used one, three or six times in simulated clinical conditions on extracted maxillary premolars. After their use the instruments were subjected to cleaning and sterilization procedures. Ultrastructure of the surface of instruments was observed under scanning electron microscope (SEM). Surface analysis was performed based on the number of uses and size of instruments. The following characteristics were analyzed: defects of instrument flutes, defects of cutting edges and instrument deformations. Results SEM analysis showed defects on the surface of non-used instruments as a result of the manufacturing process. Surface defects, defects of cutting edges as well as flutes deformations were observed after a single use and were more often noticed in small sized instruments. After three uses, flutes deformations were observed in all small sized instruments and some of the medium size. After six uses all groups of instruments showed defects of cutting edges. Conclusion Non-used stainless steel endodontic instruments showed surface defects created by the manufacturing process. Defects of cutting edges and flutes can be observed after a single use. To minimize complications and mistakes during clinical preparation it is recommended to limit the number of instrument uses.
AB  - Uvod Iako brojne studije ističu prednost korišćenja instrumenata od nikl-titanijuma zbog njihovih prednosti, primena endodontskih instrumenata od nerđajućeg čelika i dalje je standard u svakodnevnoj praksi. Cilj ovog rada je bio da se provere ultrastrukturne odlike površine radnog dela endodontskih instrumenata od nerđajućeg čelika pre i posle njihove upotrebe. Materijal i metode rada U istraživanju je korišćeno 14 setova novih ručnih proširivača i turpija od nerđajućeg čelika. Instrumenti svakog seta su korišćeni jednom, tri i šest puta za preparaciju kanala u simuliranim kliničkim uslovima na ekstrahovanim gornjim premolarima. Nakon svake upotrebe instrumenti su očišćeni i sterilizovani. Površina instrumenata je posmatrana i analizirana na skening-elektronskom mikroskopu (SEM). Analiza ultrastrukture instrumenata vršena je u pogledu broja upotreba i veličine. Kada su u pitanju ultrastrukturne promene na instrumentima, analizirani su oštećenja površine i sečiva i deformacije instrumenata. Rezultati SEM analiza je pokazala da i kod novih instrumenata postoje oštećenja koja su verovatno posledica proizvodnog procesa. Nakon prve upotrebe javljala su se oštećenja površine, sečivnih ivica, kao i deformacije navoja, i to češće kod manjih veličina. Nakon tri upotrebe deformiteti navoja su se javili kod svih instrumenata malih veličina i kod pojedinih instrumenata srednje velicine. Nakon šeste upotrebe dominirala su oštećenja u vidu tupljenja i zalamanja sečivnih ivica, koja su bila jednako zastupljena u svim grupama instrumenata. Zaključak Novi endodontski instrumenti od nerđajućegčelika imaju na svojoj površini oštećenja koja potiču od procesa proizvodnje, a deformacije instrumenata i oštećenja seciva se javljaju već posle prve kliničke upotrebe. Da bi se sprečile komplikacije i greške u kliničkom radu, preporuka je da broj upotreba endodontskih instrumenata bude ograničen.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use
T1  - Ultrastrukturna ispitivanja površine endodontskih instrumenata od nerđajućeg čelika
VL  - 61
IS  - 4
SP  - 175
EP  - 182
DO  - 10.2298/sgs1404175p
ER  - 

@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Mitić, Aleksandar and Nikolić, Marija and Barac, Radomir",
year = "2014",
abstract = "Introduction Although many studies have reported various advantages of nickel-titanium endodontic instruments, the use of stainless steel endodontic files still presents a standard in everyday practice. The aim of this study was to evaluate ultrastructure of the surface of stainless steel endodontic instruments before and after their use. Material and Methods Fourteen sets of non-used stainless steel endodontic reamers and files were included in the study. Each set of instruments was used one, three or six times in simulated clinical conditions on extracted maxillary premolars. After their use the instruments were subjected to cleaning and sterilization procedures. Ultrastructure of the surface of instruments was observed under scanning electron microscope (SEM). Surface analysis was performed based on the number of uses and size of instruments. The following characteristics were analyzed: defects of instrument flutes, defects of cutting edges and instrument deformations. Results SEM analysis showed defects on the surface of non-used instruments as a result of the manufacturing process. Surface defects, defects of cutting edges as well as flutes deformations were observed after a single use and were more often noticed in small sized instruments. After three uses, flutes deformations were observed in all small sized instruments and some of the medium size. After six uses all groups of instruments showed defects of cutting edges. Conclusion Non-used stainless steel endodontic instruments showed surface defects created by the manufacturing process. Defects of cutting edges and flutes can be observed after a single use. To minimize complications and mistakes during clinical preparation it is recommended to limit the number of instrument uses., Uvod Iako brojne studije ističu prednost korišćenja instrumenata od nikl-titanijuma zbog njihovih prednosti, primena endodontskih instrumenata od nerđajućeg čelika i dalje je standard u svakodnevnoj praksi. Cilj ovog rada je bio da se provere ultrastrukturne odlike površine radnog dela endodontskih instrumenata od nerđajućeg čelika pre i posle njihove upotrebe. Materijal i metode rada U istraživanju je korišćeno 14 setova novih ručnih proširivača i turpija od nerđajućeg čelika. Instrumenti svakog seta su korišćeni jednom, tri i šest puta za preparaciju kanala u simuliranim kliničkim uslovima na ekstrahovanim gornjim premolarima. Nakon svake upotrebe instrumenti su očišćeni i sterilizovani. Površina instrumenata je posmatrana i analizirana na skening-elektronskom mikroskopu (SEM). Analiza ultrastrukture instrumenata vršena je u pogledu broja upotreba i veličine. Kada su u pitanju ultrastrukturne promene na instrumentima, analizirani su oštećenja površine i sečiva i deformacije instrumenata. Rezultati SEM analiza je pokazala da i kod novih instrumenata postoje oštećenja koja su verovatno posledica proizvodnog procesa. Nakon prve upotrebe javljala su se oštećenja površine, sečivnih ivica, kao i deformacije navoja, i to češće kod manjih veličina. Nakon tri upotrebe deformiteti navoja su se javili kod svih instrumenata malih veličina i kod pojedinih instrumenata srednje velicine. Nakon šeste upotrebe dominirala su oštećenja u vidu tupljenja i zalamanja sečivnih ivica, koja su bila jednako zastupljena u svim grupama instrumenata. Zaključak Novi endodontski instrumenti od nerđajućegčelika imaju na svojoj površini oštećenja koja potiču od procesa proizvodnje, a deformacije instrumenata i oštećenja seciva se javljaju već posle prve kliničke upotrebe. Da bi se sprečile komplikacije i greške u kliničkom radu, preporuka je da broj upotreba endodontskih instrumenata bude ograničen.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use, Ultrastrukturna ispitivanja površine endodontskih instrumenata od nerđajućeg čelika",
volume = "61",
number = "4",
pages = "175-182",
doi = "10.2298/sgs1404175p"
}

Popović, J., Gašić, J., Živković, S., Mitić, A., Nikolić, M.,& Barac, R.. (2014). Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 61(4), 175-182.
https://doi.org/10.2298/sgs1404175p

Popović J, Gašić J, Živković S, Mitić A, Nikolić M, Barac R. Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use. in Stomatološki glasnik Srbije. 2014;61(4):175-182.
doi:10.2298/sgs1404175p .

Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Mitić, Aleksandar, Nikolić, Marija, Barac, Radomir, "Ultrastructural analysis of the surface of stainless steel endodontic instruments before and after use" in Stomatološki glasnik Srbije, 61, no. 4 (2014):175-182,
https://doi.org/10.2298/sgs1404175p . .

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Dačić, Miloš
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Tošić, Goran
AU  - Igić, Marko
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1914
AB  - Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems.
AB  - Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization
T1  - Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije
VL  - 142
IS  - 7-8
SP  - 404
EP  - 412
DO  - 10.2298/SARH1408404D
ER  - 

@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Dačić, Miloš and Radičević, Goran and Mitić, Aleksandar and Tošić, Goran and Igić, Marko",
year = "2014",
abstract = "Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems., Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization, Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije",
volume = "142",
number = "7-8",
pages = "404-412",
doi = "10.2298/SARH1408404D"
}

Dačić, S., Dačić-Simonović, D., Živković, S., Dačić, M., Radičević, G., Mitić, A., Tošić, G.,& Igić, M.. (2014). Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(7-8), 404-412.
https://doi.org/10.2298/SARH1408404D

Dačić S, Dačić-Simonović D, Živković S, Dačić M, Radičević G, Mitić A, Tošić G, Igić M. Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):404-412.
doi:10.2298/SARH1408404D .

Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Dačić, Miloš, Radičević, Goran, Mitić, Aleksandar, Tošić, Goran, Igić, Marko, "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):404-412,
https://doi.org/10.2298/SARH1408404D . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Milašin, Jelena
AU  - Živković, Slavoljub
AU  - Gašić, Jovanka
AU  - Mitić, Vladimir
AU  - Popović, Jelena
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1795
AB  - Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
AB  - Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
T1  - Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR
VL  - 141
IS  - 3-4
SP  - 155
EP  - 162
DO  - 10.2298/SARH1304155M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Milašin, Jelena and Živković, Slavoljub and Gašić, Jovanka and Mitić, Vladimir and Popović, Jelena",
year = "2013",
abstract = "Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly., Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique, Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR",
volume = "141",
number = "3-4",
pages = "155-162",
doi = "10.2298/SARH1304155M"
}

Mitić, A., Mitić, N., Milašin, J., Živković, S., Gašić, J., Mitić, V.,& Popović, J.. (2013). Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 155-162.
https://doi.org/10.2298/SARH1304155M

Mitić A, Mitić N, Milašin J, Živković S, Gašić J, Mitić V, Popović J. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):155-162.
doi:10.2298/SARH1304155M .

Mitić, Aleksandar, Mitić, Nadica, Milašin, Jelena, Živković, Slavoljub, Gašić, Jovanka, Mitić, Vladimir, Popović, Jelena, "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):155-162,
https://doi.org/10.2298/SARH1304155M . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Živković, Slavoljub
AU  - Tošić, Goran
AU  - Savić, Vojin
AU  - Dačić, Stefan
AU  - Stojanović, Miodrag
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1458
AB  - Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system.
AB  - Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Efficiency of final irrigation of root canal in removal of smear layer
T1  - Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja
VL  - 137
IS  - 9-10
SP  - 482
EP  - 489
DO  - 10.2298/SARH0910482M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Živković, Slavoljub and Tošić, Goran and Savić, Vojin and Dačić, Stefan and Stojanović, Miodrag",
year = "2009",
abstract = "Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system., Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Efficiency of final irrigation of root canal in removal of smear layer, Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja",
volume = "137",
number = "9-10",
pages = "482-489",
doi = "10.2298/SARH0910482M"
}

Mitić, A., Mitić, N., Živković, S., Tošić, G., Savić, V., Dačić, S.,& Stojanović, M.. (2009). Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 482-489.
https://doi.org/10.2298/SARH0910482M

Mitić A, Mitić N, Živković S, Tošić G, Savić V, Dačić S, Stojanović M. Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):482-489.
doi:10.2298/SARH0910482M .

Mitić, Aleksandar, Mitić, Nadica, Živković, Slavoljub, Tošić, Goran, Savić, Vojin, Dačić, Stefan, Stojanović, Miodrag, "Efficiency of final irrigation of root canal in removal of smear layer" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):482-489,
https://doi.org/10.2298/SARH0910482M . .

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Stanojević, Ivana
AU  - Veselinović, Aleksandar
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1457
AB  - Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems.
AB  - Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system
T1  - SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema
VL  - 137
IS  - 9-10
SP  - 475
EP  - 481
DO  - 10.2298/SARH0910475D
ER  - 

@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Radičević, Goran and Mitić, Aleksandar and Stanojević, Ivana and Veselinović, Aleksandar",
year = "2009",
abstract = "Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems., Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system, SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema",
volume = "137",
number = "9-10",
pages = "475-481",
doi = "10.2298/SARH0910475D"
}

Dačić, S., Dačić-Simonović, D., Živković, S., Radičević, G., Mitić, A., Stanojević, I.,& Veselinović, A.. (2009). SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 475-481.
https://doi.org/10.2298/SARH0910475D

Dačić S, Dačić-Simonović D, Živković S, Radičević G, Mitić A, Stanojević I, Veselinović A. SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):475-481.
doi:10.2298/SARH0910475D .

Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Radičević, Goran, Mitić, Aleksandar, Stanojević, Ivana, Veselinović, Aleksandar, "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):475-481,
https://doi.org/10.2298/SARH0910475D . .