Opačić-Galić, Vanja

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The influence of the final irrigation protocol on the efficiency of root canal cleaning

Nešković, Jelena; Ninković, Neda; Opačić-Galić, Vanja; Jovanović-Medojević, Milica; Popović-Bajić, Marijana; Maksimović, Miloš; Živković, Slavoljub

(Srpsko lekarsko društvo, Beograd, 2020)

TY  - JOUR
AU  - Nešković, Jelena
AU  - Ninković, Neda
AU  - Opačić-Galić, Vanja
AU  - Jovanović-Medojević, Milica
AU  - Popović-Bajić, Marijana
AU  - Maksimović, Miloš
AU  - Živković, Slavoljub
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2491
AB  - Introduction/Objective Irrigation has an important role in root canal cleaning and its efficiency depends on the type of irrigants, the amount, the technique and the irrigation protocol. The aim of this work was to estimate the efficiency of cleaning of the canal walls by using scanning electron microscope analysis after the instrumentation by rotary NiTi instruments with the use of three different irrigation solutions and two final irrigation protocols. Methods Sixty extracted human incisors were divided into two groups after the rotary instrumentation with the iRace instruments. In both groups, the same amount (1.5 ml) of three solutions (2% sodium hypochlorite solution, 2% chlorhexidine solution, and 10% citric acid solution) and total final irrigation time (90 seconds) was the same. The final irrigation in the first group was accomplished using the technique of continuous irrigation and in the second group it was done using the intermittent protocol. The roots were cut longitudinally and analyzed by thirds (coronal, middle, and apical) on a scanning electron microscope (JSM 6460LV, JEOL Ltd., Tokyo, Japan) with 1,000× magnification. Results the most efficient cleaning of the root canal walls in both groups was seen after the use of citric acid with the intermittent protocol of the final irrigation (90.7% clean walls), while the least efficient was the final irrigation by chlorhexidine with continuous irrigation (80.3%). The most efficient cleaning of the canal walls in both groups was observed in the coronal third and the largest amount of the smear layer in the apical third. Conclusion the most efficient cleaning of the canal was achieved by the use of citric acid and the intermittent protocol of the final irrigation. In all tested solutions, the intermittent protocol of irrigation was more efficient than continuous irrigation.
AB  - Uvod/Cilj Irigacija ima važnu ulogu u čišćenju kanala korena, a njena efikasnost zavisi od vrste irigansa, količine, odnosno tehnike i protokola irigacije. Cilj ovog rada bio je da se analizom SEM proceni efikasnost čišćenja zidova kanala posle instrumentacije rotirajućim NiTi instrumentima uz primenu tri različita rastvora za irigaciju i dva protokola finalne irigacije. Metode Šezdeset ekstrahovanih humanih sekutića je posle mašinske instrumentacije iRaCe instrumentima podeljeno u dve grupe. U obe grupe su korišćena po tri rastvora - 2% rastvor natrijum-hipohlorita, 2% rastvor hlorheksidina i 10% rastvor limunske kiseline, u istoj količini (1,5 ml) i ukupnom vremenu finalne irigacije (90 sekundi). Finalna irigacija u prvoj grupi je realizovana tehnikom kontinuirane, a u drugoj tehnikom intermitentne irigacije. Korenovi su presečeni uzdužno i analizirani po trećinama (krunična, srednja i apikalna) na skenirajućem elektronskom mikroskopu (JSM 6460LV JEOL, Tokio, Japan) na uveličanju od 1000×. Rezultati Najefikasnije čišćenje zidova kanala korena u obe grupe uočeno je posle primene limunske kiseline uz intermitentni protokol finalne irigacije (90,7% čistih zidova), dok je najmanje efikasna bila finalna irigacija hlorheksidinom uz kontinuiranu irigaciju (80,3%). Najefikasnije čišćenje zidova kanala i u prvoj i u drugoj grupi uočeno je u kruničnoj trećini, a najviše razmaznog sloja u apikalnoj trećini. Zaključak Najefikasnije čišćenje kanala ostvareno je primenom limunske kiseline i intermitentnog protokola finalne irigacije. Kod svih testiranih rastvora intermitentni protokol irigacije je bio nešto efikasniji od protokola kontinuirane irigacije.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The influence of the final irrigation protocol on the efficiency of root canal cleaning
T1  - Uticaj protokola finalne irigacije na efikasnost čišćenja kanala korena
VL  - 148
IS  - 1-2
SP  - 10
EP  - 16
DO  - 10.2298/SARH190513132N
ER  - 
@article{
author = "Nešković, Jelena and Ninković, Neda and Opačić-Galić, Vanja and Jovanović-Medojević, Milica and Popović-Bajić, Marijana and Maksimović, Miloš and Živković, Slavoljub",
year = "2020",
abstract = "Introduction/Objective Irrigation has an important role in root canal cleaning and its efficiency depends on the type of irrigants, the amount, the technique and the irrigation protocol. The aim of this work was to estimate the efficiency of cleaning of the canal walls by using scanning electron microscope analysis after the instrumentation by rotary NiTi instruments with the use of three different irrigation solutions and two final irrigation protocols. Methods Sixty extracted human incisors were divided into two groups after the rotary instrumentation with the iRace instruments. In both groups, the same amount (1.5 ml) of three solutions (2% sodium hypochlorite solution, 2% chlorhexidine solution, and 10% citric acid solution) and total final irrigation time (90 seconds) was the same. The final irrigation in the first group was accomplished using the technique of continuous irrigation and in the second group it was done using the intermittent protocol. The roots were cut longitudinally and analyzed by thirds (coronal, middle, and apical) on a scanning electron microscope (JSM 6460LV, JEOL Ltd., Tokyo, Japan) with 1,000× magnification. Results the most efficient cleaning of the root canal walls in both groups was seen after the use of citric acid with the intermittent protocol of the final irrigation (90.7% clean walls), while the least efficient was the final irrigation by chlorhexidine with continuous irrigation (80.3%). The most efficient cleaning of the canal walls in both groups was observed in the coronal third and the largest amount of the smear layer in the apical third. Conclusion the most efficient cleaning of the canal was achieved by the use of citric acid and the intermittent protocol of the final irrigation. In all tested solutions, the intermittent protocol of irrigation was more efficient than continuous irrigation., Uvod/Cilj Irigacija ima važnu ulogu u čišćenju kanala korena, a njena efikasnost zavisi od vrste irigansa, količine, odnosno tehnike i protokola irigacije. Cilj ovog rada bio je da se analizom SEM proceni efikasnost čišćenja zidova kanala posle instrumentacije rotirajućim NiTi instrumentima uz primenu tri različita rastvora za irigaciju i dva protokola finalne irigacije. Metode Šezdeset ekstrahovanih humanih sekutića je posle mašinske instrumentacije iRaCe instrumentima podeljeno u dve grupe. U obe grupe su korišćena po tri rastvora - 2% rastvor natrijum-hipohlorita, 2% rastvor hlorheksidina i 10% rastvor limunske kiseline, u istoj količini (1,5 ml) i ukupnom vremenu finalne irigacije (90 sekundi). Finalna irigacija u prvoj grupi je realizovana tehnikom kontinuirane, a u drugoj tehnikom intermitentne irigacije. Korenovi su presečeni uzdužno i analizirani po trećinama (krunična, srednja i apikalna) na skenirajućem elektronskom mikroskopu (JSM 6460LV JEOL, Tokio, Japan) na uveličanju od 1000×. Rezultati Najefikasnije čišćenje zidova kanala korena u obe grupe uočeno je posle primene limunske kiseline uz intermitentni protokol finalne irigacije (90,7% čistih zidova), dok je najmanje efikasna bila finalna irigacija hlorheksidinom uz kontinuiranu irigaciju (80,3%). Najefikasnije čišćenje zidova kanala i u prvoj i u drugoj grupi uočeno je u kruničnoj trećini, a najviše razmaznog sloja u apikalnoj trećini. Zaključak Najefikasnije čišćenje kanala ostvareno je primenom limunske kiseline i intermitentnog protokola finalne irigacije. Kod svih testiranih rastvora intermitentni protokol irigacije je bio nešto efikasniji od protokola kontinuirane irigacije.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The influence of the final irrigation protocol on the efficiency of root canal cleaning, Uticaj protokola finalne irigacije na efikasnost čišćenja kanala korena",
volume = "148",
number = "1-2",
pages = "10-16",
doi = "10.2298/SARH190513132N"
}
Nešković, J., Ninković, N., Opačić-Galić, V., Jovanović-Medojević, M., Popović-Bajić, M., Maksimović, M.,& Živković, S.. (2020). The influence of the final irrigation protocol on the efficiency of root canal cleaning. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 148(1-2), 10-16.
https://doi.org/10.2298/SARH190513132N
Nešković J, Ninković N, Opačić-Galić V, Jovanović-Medojević M, Popović-Bajić M, Maksimović M, Živković S. The influence of the final irrigation protocol on the efficiency of root canal cleaning. in Srpski arhiv za celokupno lekarstvo. 2020;148(1-2):10-16.
doi:10.2298/SARH190513132N .
Nešković, Jelena, Ninković, Neda, Opačić-Galić, Vanja, Jovanović-Medojević, Milica, Popović-Bajić, Marijana, Maksimović, Miloš, Živković, Slavoljub, "The influence of the final irrigation protocol on the efficiency of root canal cleaning" in Srpski arhiv za celokupno lekarstvo, 148, no. 1-2 (2020):10-16,
https://doi.org/10.2298/SARH190513132N . .
3
2

Gloss and surface analysis of various composite materials

Opačić-Galić, Vanja; Popović, Danica; Stasić, Jovana; Dželetović, Bojan; Manojlović, Stefan

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2019)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Popović, Danica
AU  - Stasić, Jovana
AU  - Dželetović, Bojan
AU  - Manojlović, Stefan
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2449
AB  - Introduction To obtain the ideal aesthetics, each restorative material must imitate natural tooth in color, surface texture and be stable over time. Damage or wear of the material causes poor optical properties of the restoration, so it is necessary to repolish, repair or replace it. The aim of this study was to test the gloss and surface changes of the composite materials with filler's particles of different size, before and after material artificial mechanical ageing. Material and Methods Four composite materials were tested, two microhybrid composites (Gradia Direct GC, Herculite XRV, Kerr) and two nanohybrid composites (Filtek Ultimate 3M; TetricEvoceram, Ivoclar). Composites' samples of 9 × 9 × 2 mm in size were polished in accordance to the standard protocol by Sof-Lex discs (2382 C, SM, F, SF) for 30 seconds. Gloss was measured after polishing and taking photos using optical microscope (400×). In the chewing simulator, ageing of the samples was conducted (100,000 cyclic kicks). After the samples' ageing, surface photos were taken and gloss measured. The obtained results were statistically processed (One-way ANOVA, t-test). Results The best gloss after polishing was shown by Filtek Ultimate (54.00 ± 14.06), the worst by Gradia Direct (47.33 ± 7.92). There was no statistically significant difference (p > 0.05) in surface gloss after polishing composite. The smallest defects on the surface of composites were detected in material Gradia Direct (21363.7 mm2 ) with the average value of the diameter 137 mm. Conclusion The tested nanohybrid and microhybrid composites showed comparable gloss value before and after artificial aging. Mechanical aging caused vivid changes regarding surface defects on all tested composite materials.
AB  - Uvod Da bi se dobila idealna estetika, svaki restaurativni materijal mora oponašati prirodni zub po boji i teksturi površine i mora biti stabilan u vremenu. Oštećenje ili habanje materijala uzrokuje loša optička svojstva ispuna, pa ga je neophodno prepolirati, reparirati ili zameniti. Cilj rada je bio da se ispitaju sjaj i površinske promene kompozita sa različitom veličinom čestica punilaca, pre i posle artificijalnog mehaničkog starenja. Materijal i metode Testirana su četiri kompozita, dva mikrohibridna (Gradia Direct GC; Herculite XRV, Kerr) i dva nanohibridna (Filtek Ultimate 3M; Tetric Evoceram, Ivoclar). Uzorci kompozita (9 × 9 × 2 mm) polirani su po standardnom protokolu diskovima Sof-Lex 30 sek. Posle poliranja izmeren je sjaj, a uzorci su posmatrani pod optičkim mikroskopom (×400). U simulatoru žvakanja uzorci su izloženi artificijalnom starenju sa 100.000 cikličnih udaraca. Posle mehaničkog starenja ponovo je izmeren sjaj, a površine su fotografisane i sačuvane u digitalnom formatu. Dobijeni rezultati su statistički obrađeni (One-way ANOVA, t-test). Rezultati Najveći sjaj posle poliranja pokazao je Filtek Ultimate (54,00 ± 14,06), a najmanji Gradia Direct (47,33 ± 7,92). Nije bilo statistički značajne razlike sjaja između testiranih materijala posle poliranja (p > 0,05). Najmanji defekti na površini kompozita uočeni su kod materijala Gradia Direct (21.364 mm), sa srednjom vrednošću prečnika 137 mm. Zaključak Ispitivani nanohibridni i mikrohibridni kompozitisu pokazali komparabilne vrednosti sjaja pre i posle artificijalnog starenja. Mehaničko starenje je izazvalo vidljive promene u vidu površinskih defekata na svim ispitivanim kompozitima.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Gloss and surface analysis of various composite materials
T1  - Analiza sjaja i površine različitih kompozitnih materijala
VL  - 66
IS  - 4
SP  - 167
EP  - 174
DO  - 10.2478/sdj-2019-0017
ER  - 
@article{
author = "Opačić-Galić, Vanja and Popović, Danica and Stasić, Jovana and Dželetović, Bojan and Manojlović, Stefan",
year = "2019",
abstract = "Introduction To obtain the ideal aesthetics, each restorative material must imitate natural tooth in color, surface texture and be stable over time. Damage or wear of the material causes poor optical properties of the restoration, so it is necessary to repolish, repair or replace it. The aim of this study was to test the gloss and surface changes of the composite materials with filler's particles of different size, before and after material artificial mechanical ageing. Material and Methods Four composite materials were tested, two microhybrid composites (Gradia Direct GC, Herculite XRV, Kerr) and two nanohybrid composites (Filtek Ultimate 3M; TetricEvoceram, Ivoclar). Composites' samples of 9 × 9 × 2 mm in size were polished in accordance to the standard protocol by Sof-Lex discs (2382 C, SM, F, SF) for 30 seconds. Gloss was measured after polishing and taking photos using optical microscope (400×). In the chewing simulator, ageing of the samples was conducted (100,000 cyclic kicks). After the samples' ageing, surface photos were taken and gloss measured. The obtained results were statistically processed (One-way ANOVA, t-test). Results The best gloss after polishing was shown by Filtek Ultimate (54.00 ± 14.06), the worst by Gradia Direct (47.33 ± 7.92). There was no statistically significant difference (p > 0.05) in surface gloss after polishing composite. The smallest defects on the surface of composites were detected in material Gradia Direct (21363.7 mm2 ) with the average value of the diameter 137 mm. Conclusion The tested nanohybrid and microhybrid composites showed comparable gloss value before and after artificial aging. Mechanical aging caused vivid changes regarding surface defects on all tested composite materials., Uvod Da bi se dobila idealna estetika, svaki restaurativni materijal mora oponašati prirodni zub po boji i teksturi površine i mora biti stabilan u vremenu. Oštećenje ili habanje materijala uzrokuje loša optička svojstva ispuna, pa ga je neophodno prepolirati, reparirati ili zameniti. Cilj rada je bio da se ispitaju sjaj i površinske promene kompozita sa različitom veličinom čestica punilaca, pre i posle artificijalnog mehaničkog starenja. Materijal i metode Testirana su četiri kompozita, dva mikrohibridna (Gradia Direct GC; Herculite XRV, Kerr) i dva nanohibridna (Filtek Ultimate 3M; Tetric Evoceram, Ivoclar). Uzorci kompozita (9 × 9 × 2 mm) polirani su po standardnom protokolu diskovima Sof-Lex 30 sek. Posle poliranja izmeren je sjaj, a uzorci su posmatrani pod optičkim mikroskopom (×400). U simulatoru žvakanja uzorci su izloženi artificijalnom starenju sa 100.000 cikličnih udaraca. Posle mehaničkog starenja ponovo je izmeren sjaj, a površine su fotografisane i sačuvane u digitalnom formatu. Dobijeni rezultati su statistički obrađeni (One-way ANOVA, t-test). Rezultati Najveći sjaj posle poliranja pokazao je Filtek Ultimate (54,00 ± 14,06), a najmanji Gradia Direct (47,33 ± 7,92). Nije bilo statistički značajne razlike sjaja između testiranih materijala posle poliranja (p > 0,05). Najmanji defekti na površini kompozita uočeni su kod materijala Gradia Direct (21.364 mm), sa srednjom vrednošću prečnika 137 mm. Zaključak Ispitivani nanohibridni i mikrohibridni kompozitisu pokazali komparabilne vrednosti sjaja pre i posle artificijalnog starenja. Mehaničko starenje je izazvalo vidljive promene u vidu površinskih defekata na svim ispitivanim kompozitima.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Gloss and surface analysis of various composite materials, Analiza sjaja i površine različitih kompozitnih materijala",
volume = "66",
number = "4",
pages = "167-174",
doi = "10.2478/sdj-2019-0017"
}
Opačić-Galić, V., Popović, D., Stasić, J., Dželetović, B.,& Manojlović, S.. (2019). Gloss and surface analysis of various composite materials. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 66(4), 167-174.
https://doi.org/10.2478/sdj-2019-0017
Opačić-Galić V, Popović D, Stasić J, Dželetović B, Manojlović S. Gloss and surface analysis of various composite materials. in Stomatološki glasnik Srbije. 2019;66(4):167-174.
doi:10.2478/sdj-2019-0017 .
Opačić-Galić, Vanja, Popović, Danica, Stasić, Jovana, Dželetović, Bojan, Manojlović, Stefan, "Gloss and surface analysis of various composite materials" in Stomatološki glasnik Srbije, 66, no. 4 (2019):167-174,
https://doi.org/10.2478/sdj-2019-0017 . .

Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite

Opačić-Galić, Vanja; Petrović, Violeta; Popović-Bajić, Marijana; Jokanović, Vukoman; Živković, Slavoljub; Nikolić, Biljana; Mitić-Ćulafić, Dragana

(Springer International Publishing, 2018)

TY  - CHAP
AU  - Opačić-Galić, Vanja
AU  - Petrović, Violeta
AU  - Popović-Bajić, Marijana
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
AU  - Nikolić, Biljana
AU  - Mitić-Ćulafić, Dragana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2371
PB  - Springer International Publishing
T2  - Dental Applications of Nanotechnology
T1  - Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite
SP  - 247
EP  - 271
DO  - 10.1007/978-3-319-97634-1_13
ER  - 
@inbook{
author = "Opačić-Galić, Vanja and Petrović, Violeta and Popović-Bajić, Marijana and Jokanović, Vukoman and Živković, Slavoljub and Nikolić, Biljana and Mitić-Ćulafić, Dragana",
year = "2018",
publisher = "Springer International Publishing",
journal = "Dental Applications of Nanotechnology",
booktitle = "Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite",
pages = "247-271",
doi = "10.1007/978-3-319-97634-1_13"
}
Opačić-Galić, V., Petrović, V., Popović-Bajić, M., Jokanović, V., Živković, S., Nikolić, B.,& Mitić-Ćulafić, D.. (2018). Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. in Dental Applications of Nanotechnology
Springer International Publishing., 247-271.
https://doi.org/10.1007/978-3-319-97634-1_13
Opačić-Galić V, Petrović V, Popović-Bajić M, Jokanović V, Živković S, Nikolić B, Mitić-Ćulafić D. Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. in Dental Applications of Nanotechnology. 2018;:247-271.
doi:10.1007/978-3-319-97634-1_13 .
Opačić-Galić, Vanja, Petrović, Violeta, Popović-Bajić, Marijana, Jokanović, Vukoman, Živković, Slavoljub, Nikolić, Biljana, Mitić-Ćulafić, Dragana, "Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite" in Dental Applications of Nanotechnology (2018):247-271,
https://doi.org/10.1007/978-3-319-97634-1_13 . .
2
1

Compressive strength of calcium silicate-based cement

Opačić-Galić, Vanja; Stamenić, Zoran; Petrović, Violeta; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2018)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Stamenić, Zoran
AU  - Petrović, Violeta
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2358
AB  - Introduction The aim of this study was to compare compressive strength (Cs) of new nanostructural calcium silicate based cement (nCS) with commercial calcium silicate cement and conventional GIC. Methods Four nanostructural materials were tested: nanostructural calcium silicate based cement (nCS) (Jokanović et al.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Five samples of each material were mixed in accordance with manifecturer's guidelines and positioned in metal moulds (ϕ4mm and 6mm). Compressive strength (Cs) expressed in MPa was determined after 24 hours, 7 days and 28 days respectively. Measurements were performed on universal testing equipment (Tinius Olsen, USA) at a crosshead speed of 1mm/min. For processing the results one-way ANOVA and post-hoc test were used. Results The highest values of compressive strength after 24h was found in conventional GIC Fuji IX (mean 38.56±13.31) and Ketac Universal (mean 40.77±7.96). Calcium silicate cements after 24h showed low values of compressive strength (MTA Plus 5.91±0.28 MPa, nCS 1.35±0.36 MPa). After 7 days, FUJI IX 47.42±9.33 MPa and Ketac Universal 35.25±10.60 MPa showed higher value of compressive strength than MTA Plus (15.09±2.77 MPa) and nCS (11.06±0.88 MPa). After 28 days the Cs value for conventional GIC Fuji IX was 48.03±7.82 MPa and Ketac Universal 36.65±11.13 MPa while for calcium silicate cements it was 16.47±1.89 MPa and nCS 14.39±1.63 MPa. There was statistically significant difference (p lt 0.05) in Cs between conventional GIC and CS cements after 24h, 7 and 28 days. Conclusions Calcium silicate cements initially showed lower values of compressive strength than conventional GIC that increased over time.
AB  - Uvod Cilj ovog rada je bio da se proveri kompresivna čvrstoća (KČ) novog nanostrukturnog kalcijum-silikatnog cementa (nCS) i uporedi sa komercijalnim kalcijum-silikatnim cementom i konvencionalnim GJC u funkciji vremena. Materijal i metod Testirana su četiri materijala - nanostrukturni CS (Jokanović i sar.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Po pet uzoraka za svaki materijal je zamešano po proizvođačkom uputstvu i postavljano u metalne kalupe (ϕ 4 mm i 6 mm visoke). KČ, izražena u megapaskalima, merena je posle 24 sata i posle 7 i 28 dana na univerzalnoj test mašini (Tinius Olsen, USA) sa brzinom utiskivača od 1 mm/min. Dobijeni rezultati su statistički obrađeni one-way ANOVA i post hoc Tukey, s testovima. Resultati Posle 24 sata najveću KČ imao je FUJI IX (38,56 ± 13,31 MPa), zatim Ketac Univerzal (40,77 ± 7,96 MPa). Kalcijum-silikatni cementi su pokazali niže vrednosti KČ 24 sata posle mešanja i to MTA 5,91 ± 0,28, a nCS 1,35 ± 0,36 MPa. Posle sedam dana KČ za FUJI IX je bila 47,72 ± 9,33 MPa, a za Ketac Universal 35,25 ± 10,60 MPa, dok je vrednost za MTA bila 15,09 ± 2,77 MPa, a nCS 11,06 ± 0,88. Posle 28 dana KČ za FUJI IX je bila 48,03 ± 7,82 MPa, a za Ketac 36,65 ± 11,13 MPa. KČ kalcijum-silikatnih cemenata posle 28 dana je bila 16,47 ± 1,89 za MTA, a za nCS 14,39 ± 1,63 MPa, bez statističi značajne razlike između njih. Između konvencionalnih GJC i CS cemenata postoji statistički značajna razlika (p  lt  0,05) posle 24 h, kao i posle 7 i 28 dana. Zaključak Kalcijum-silikatni cementi su inicijalno pokazali niže vrednosti KČ u odnosu na konvencionalne GJC, ali su se one povećavale u funkciji vremena.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Compressive strength of calcium silicate-based cement
T1  - Ispitivanje kompresivne čvrstoće kalcijum-silikatnih cemenata
VL  - 65
IS  - 1
SP  - 7
EP  - 13
DO  - 10.2478/sdj-2018-0001
ER  - 
@article{
author = "Opačić-Galić, Vanja and Stamenić, Zoran and Petrović, Violeta and Jokanović, Vukoman and Živković, Slavoljub",
year = "2018",
abstract = "Introduction The aim of this study was to compare compressive strength (Cs) of new nanostructural calcium silicate based cement (nCS) with commercial calcium silicate cement and conventional GIC. Methods Four nanostructural materials were tested: nanostructural calcium silicate based cement (nCS) (Jokanović et al.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Five samples of each material were mixed in accordance with manifecturer's guidelines and positioned in metal moulds (ϕ4mm and 6mm). Compressive strength (Cs) expressed in MPa was determined after 24 hours, 7 days and 28 days respectively. Measurements were performed on universal testing equipment (Tinius Olsen, USA) at a crosshead speed of 1mm/min. For processing the results one-way ANOVA and post-hoc test were used. Results The highest values of compressive strength after 24h was found in conventional GIC Fuji IX (mean 38.56±13.31) and Ketac Universal (mean 40.77±7.96). Calcium silicate cements after 24h showed low values of compressive strength (MTA Plus 5.91±0.28 MPa, nCS 1.35±0.36 MPa). After 7 days, FUJI IX 47.42±9.33 MPa and Ketac Universal 35.25±10.60 MPa showed higher value of compressive strength than MTA Plus (15.09±2.77 MPa) and nCS (11.06±0.88 MPa). After 28 days the Cs value for conventional GIC Fuji IX was 48.03±7.82 MPa and Ketac Universal 36.65±11.13 MPa while for calcium silicate cements it was 16.47±1.89 MPa and nCS 14.39±1.63 MPa. There was statistically significant difference (p lt 0.05) in Cs between conventional GIC and CS cements after 24h, 7 and 28 days. Conclusions Calcium silicate cements initially showed lower values of compressive strength than conventional GIC that increased over time., Uvod Cilj ovog rada je bio da se proveri kompresivna čvrstoća (KČ) novog nanostrukturnog kalcijum-silikatnog cementa (nCS) i uporedi sa komercijalnim kalcijum-silikatnim cementom i konvencionalnim GJC u funkciji vremena. Materijal i metod Testirana su četiri materijala - nanostrukturni CS (Jokanović i sar.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Po pet uzoraka za svaki materijal je zamešano po proizvođačkom uputstvu i postavljano u metalne kalupe (ϕ 4 mm i 6 mm visoke). KČ, izražena u megapaskalima, merena je posle 24 sata i posle 7 i 28 dana na univerzalnoj test mašini (Tinius Olsen, USA) sa brzinom utiskivača od 1 mm/min. Dobijeni rezultati su statistički obrađeni one-way ANOVA i post hoc Tukey, s testovima. Resultati Posle 24 sata najveću KČ imao je FUJI IX (38,56 ± 13,31 MPa), zatim Ketac Univerzal (40,77 ± 7,96 MPa). Kalcijum-silikatni cementi su pokazali niže vrednosti KČ 24 sata posle mešanja i to MTA 5,91 ± 0,28, a nCS 1,35 ± 0,36 MPa. Posle sedam dana KČ za FUJI IX je bila 47,72 ± 9,33 MPa, a za Ketac Universal 35,25 ± 10,60 MPa, dok je vrednost za MTA bila 15,09 ± 2,77 MPa, a nCS 11,06 ± 0,88. Posle 28 dana KČ za FUJI IX je bila 48,03 ± 7,82 MPa, a za Ketac 36,65 ± 11,13 MPa. KČ kalcijum-silikatnih cemenata posle 28 dana je bila 16,47 ± 1,89 za MTA, a za nCS 14,39 ± 1,63 MPa, bez statističi značajne razlike između njih. Između konvencionalnih GJC i CS cemenata postoji statistički značajna razlika (p  lt  0,05) posle 24 h, kao i posle 7 i 28 dana. Zaključak Kalcijum-silikatni cementi su inicijalno pokazali niže vrednosti KČ u odnosu na konvencionalne GJC, ali su se one povećavale u funkciji vremena.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Compressive strength of calcium silicate-based cement, Ispitivanje kompresivne čvrstoće kalcijum-silikatnih cemenata",
volume = "65",
number = "1",
pages = "7-13",
doi = "10.2478/sdj-2018-0001"
}
Opačić-Galić, V., Stamenić, Z., Petrović, V., Jokanović, V.,& Živković, S.. (2018). Compressive strength of calcium silicate-based cement. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(1), 7-13.
https://doi.org/10.2478/sdj-2018-0001
Opačić-Galić V, Stamenić Z, Petrović V, Jokanović V, Živković S. Compressive strength of calcium silicate-based cement. in Stomatološki glasnik Srbije. 2018;65(1):7-13.
doi:10.2478/sdj-2018-0001 .
Opačić-Galić, Vanja, Stamenić, Zoran, Petrović, Violeta, Jokanović, Vukoman, Živković, Slavoljub, "Compressive strength of calcium silicate-based cement" in Stomatološki glasnik Srbije, 65, no. 1 (2018):7-13,
https://doi.org/10.2478/sdj-2018-0001 . .

Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal

Opačić-Galić, Vanja; Stasić, Jovana

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2018)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Stasić, Jovana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2363
AB  - Introduction Calcium hydroxide (CH) is a medicament widely used in endodontic treatment due to its antibacterial, regenerative and biocompatible properties. Studies have shown that remaining CH on root canal walls and dentinal tubules can compromise sealer penetration, leading to its weak adhesion, volume change and, consequently, apical leakage. The aim of this study was to compare the efficacy of four different techniques in removing calcium hydroxide from the root canal. Material and Methods 32 extracted single-rooted teeth with one canal were used in this study. The canals were prepared using BioRaCe system (FKG Dentaire, Swiss) BR5 40/.04 with sodium hypochlorite irrigation after each instrument. Longitudinal grooves were formed on the proximal root surfaces. All canals (except negative control) were filled with aqueous CH suspension. After seven days of incubation, the teeth were allocated into the four groups (n=7), plus positive and negative control. Four techniques (systems) for CH removal were tested: conventional syringe irrigation (CSI), passive ultrasonic irrigation (PUI), XP-endo Finisher (FKG Dentaire, Swiss) and Canal Brush (Roeko, Coltene) with irrigation of 5 ml 2% NaOCl and 5 ml 10% citric acid. All the roots were then split into the two halves with chisel and observed under the stereomicroscope (Boeco, Germany) at magnification of 20x. The area with remaining CH on the root canal wall surface was then divided with the total root canal surface area (%). The obtained results were statistically processed using One-way ANOVA and Tukey post-hoc test (p lt 0.05). Results The most efficient system was XP-endo Finisher with 91.33% of clean surface, followed by PUI 88.36%, Canal Brush 87.83%, and CSI with 66.92%. Conclusion None of the systems completely removed the traces of the medicament from the root canal. For optimal clinical success, it is necessary to combine various systems with copious irrigation.
AB  - Uvod Kalcijum-hidroksid (CH) predstavlja medikament koji se u velikoj meri koristi u endodontskoj terapiji zbog svojih antibakterijskih, regenerativnih i biokompatibilnih svojstava. Istraživanja pokazuju da ostaci CH na dentinskim zidovima i u tubulima mogu kompromitovati prodor silera, što doprinosi njegovoj lošijoj adheziji, promeni volumena, a samim tim i apeksnom curenju. Cilj rada je bio da se uporedi efikasnost četiri različite tehnike uklanjanja kalcijum-hidroksida iz kanala korena zuba. Materijal i metode Korišćena su 32 ekstrahovana jednokorena zuba sa jednim kanalom. Kanali su mašinski obrađeni BioRaCe sistemom (FKG Dentaire, Swiss) BR5 40/.04 uz irigaciju natrijum-hipohloritom posle svakog instrumenta. Na aproksimalnim površinama korenova napravljeni su uzdužni žlebovi. Svi kanali (osim negativne kontrole) napunjeni su vodenom suspenzijom CH. Posle sedam dana u inkubatoru, zubi su podeljeni u četiri grupe (n = 7), uz pozitivnu i negativnu kontrolu. Testirane su četiri tehnike (sistema) za ukljanjanje CH iz kanala korena: konvencionalna irigacija špricem (CSI), pasivna ultrazvučna irigacija (PUI), XP-endo Finisher (FKG Dentaire, Swiss) i Canal Brush (Roeko, Coltene), uz irigaciju 5 ml 2% NaOCl i 5 ml 10% limunskom kiselinom. Svi korenovi su dletom podeljeni na dve polovine i posmatrani pod stereomikroskopom (Boeco, Germany) na uveličanju od 20×. Od vrednosti ukupne površine kanala korena oduzimane su vrednosti neočišćene površine (%). Dobijene vrednosti su statistički obrađene oneway ANOVA i Tukey post hoc testom (p  lt  0,05). Rezultati Najefikasniji sistem bio je XP-endo Finisher sa 91,33%, zatim PUI sa 88,36%, Canal Brush sa 87,83%, i konvencionalna irigacija špricem sa 66,92% očišćene površine kanala korena. Zaključak Nijedan sistem nije u potpunosti uklonio tragove medikamenta iz kanala korena. Za potpuni klinički uspeh neophodno je kombinovati više sistema uz obilnu irigaciju.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal
T1  - Efikasnost različitih tehnika irigacije u uklanjanju kalcijum-hidroksida iz kanala korena
VL  - 65
IS  - 3
SP  - 148
EP  - 155
DO  - 10.2478/sdj-2018-0015
ER  - 
@article{
author = "Opačić-Galić, Vanja and Stasić, Jovana",
year = "2018",
abstract = "Introduction Calcium hydroxide (CH) is a medicament widely used in endodontic treatment due to its antibacterial, regenerative and biocompatible properties. Studies have shown that remaining CH on root canal walls and dentinal tubules can compromise sealer penetration, leading to its weak adhesion, volume change and, consequently, apical leakage. The aim of this study was to compare the efficacy of four different techniques in removing calcium hydroxide from the root canal. Material and Methods 32 extracted single-rooted teeth with one canal were used in this study. The canals were prepared using BioRaCe system (FKG Dentaire, Swiss) BR5 40/.04 with sodium hypochlorite irrigation after each instrument. Longitudinal grooves were formed on the proximal root surfaces. All canals (except negative control) were filled with aqueous CH suspension. After seven days of incubation, the teeth were allocated into the four groups (n=7), plus positive and negative control. Four techniques (systems) for CH removal were tested: conventional syringe irrigation (CSI), passive ultrasonic irrigation (PUI), XP-endo Finisher (FKG Dentaire, Swiss) and Canal Brush (Roeko, Coltene) with irrigation of 5 ml 2% NaOCl and 5 ml 10% citric acid. All the roots were then split into the two halves with chisel and observed under the stereomicroscope (Boeco, Germany) at magnification of 20x. The area with remaining CH on the root canal wall surface was then divided with the total root canal surface area (%). The obtained results were statistically processed using One-way ANOVA and Tukey post-hoc test (p lt 0.05). Results The most efficient system was XP-endo Finisher with 91.33% of clean surface, followed by PUI 88.36%, Canal Brush 87.83%, and CSI with 66.92%. Conclusion None of the systems completely removed the traces of the medicament from the root canal. For optimal clinical success, it is necessary to combine various systems with copious irrigation., Uvod Kalcijum-hidroksid (CH) predstavlja medikament koji se u velikoj meri koristi u endodontskoj terapiji zbog svojih antibakterijskih, regenerativnih i biokompatibilnih svojstava. Istraživanja pokazuju da ostaci CH na dentinskim zidovima i u tubulima mogu kompromitovati prodor silera, što doprinosi njegovoj lošijoj adheziji, promeni volumena, a samim tim i apeksnom curenju. Cilj rada je bio da se uporedi efikasnost četiri različite tehnike uklanjanja kalcijum-hidroksida iz kanala korena zuba. Materijal i metode Korišćena su 32 ekstrahovana jednokorena zuba sa jednim kanalom. Kanali su mašinski obrađeni BioRaCe sistemom (FKG Dentaire, Swiss) BR5 40/.04 uz irigaciju natrijum-hipohloritom posle svakog instrumenta. Na aproksimalnim površinama korenova napravljeni su uzdužni žlebovi. Svi kanali (osim negativne kontrole) napunjeni su vodenom suspenzijom CH. Posle sedam dana u inkubatoru, zubi su podeljeni u četiri grupe (n = 7), uz pozitivnu i negativnu kontrolu. Testirane su četiri tehnike (sistema) za ukljanjanje CH iz kanala korena: konvencionalna irigacija špricem (CSI), pasivna ultrazvučna irigacija (PUI), XP-endo Finisher (FKG Dentaire, Swiss) i Canal Brush (Roeko, Coltene), uz irigaciju 5 ml 2% NaOCl i 5 ml 10% limunskom kiselinom. Svi korenovi su dletom podeljeni na dve polovine i posmatrani pod stereomikroskopom (Boeco, Germany) na uveličanju od 20×. Od vrednosti ukupne površine kanala korena oduzimane su vrednosti neočišćene površine (%). Dobijene vrednosti su statistički obrađene oneway ANOVA i Tukey post hoc testom (p  lt  0,05). Rezultati Najefikasniji sistem bio je XP-endo Finisher sa 91,33%, zatim PUI sa 88,36%, Canal Brush sa 87,83%, i konvencionalna irigacija špricem sa 66,92% očišćene površine kanala korena. Zaključak Nijedan sistem nije u potpunosti uklonio tragove medikamenta iz kanala korena. Za potpuni klinički uspeh neophodno je kombinovati više sistema uz obilnu irigaciju.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal, Efikasnost različitih tehnika irigacije u uklanjanju kalcijum-hidroksida iz kanala korena",
volume = "65",
number = "3",
pages = "148-155",
doi = "10.2478/sdj-2018-0015"
}
Opačić-Galić, V.,& Stasić, J.. (2018). Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(3), 148-155.
https://doi.org/10.2478/sdj-2018-0015
Opačić-Galić V, Stasić J. Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal. in Stomatološki glasnik Srbije. 2018;65(3):148-155.
doi:10.2478/sdj-2018-0015 .
Opačić-Galić, Vanja, Stasić, Jovana, "Efficacy of different irrigation techniques on calcium hydroxide removal from the root canal" in Stomatološki glasnik Srbije, 65, no. 3 (2018):148-155,
https://doi.org/10.2478/sdj-2018-0015 . .
1

Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study

Opačić-Galić, Vanja; Petrović, Violeta; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo, Beograd, 2017)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Petrović, Violeta
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2261
AB  - Introduction/Objective: Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatite-based (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods: The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results: Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ± 0.48), while CS and CS-HA scored 3 ± 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ± 0.45). The least visible inflammatory reaction of the rabbits’ pulp tissue was spotted with the CS (1.83 ± 0.75), than with the MTA and CS-HA (2.67 ± 1.53, 3 ± 0.63). Conclusion: The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits’ pulp tissue only in the immediate vicinity of the implanted material.
AB  - Uvod/Cilj: Usavršavanje materijala koji bi mogli da se koriste kao biološke zamene kosti jedna je od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Cilj ovog rada je bio da se ispita odgovor tkiva na novosintetisane nanomaterijale na bazi kalcijum-silikatnih sistema (KS) i hidroksiapatita (KS-HA) posle implantacije u potkožno tkivo pacova i direktnog prekrivanja pulpe zuba kunića. Metode: U potkožno tkivo 40 vistar pacova su implantirani testirani materijali, a posle 7, 15, 30 i 60 dana životinje su žrtvovane. Direktno prekrivanje pulpe je realizovano na zubima kunića. Na vestibularnim površinama sekutića preparisani su kaviteti, a eksponirana pulpa je prekrivana testiranim materijalima. Životinje su žrtvovane posle 10 i 15 dana. Kontrolni materijal u oba eksperimenta je bio mineralni trioksidni agregat (MTA). Histološka analiza je obuhvatila praćenje ćelijske komponente zapaljenja, prisustva gigantskih ćelija i nekroze tkiva. Rezultati: Sedam dana posle supkutane implantacije najjači zapaljenski odgovor dao je MTA (3,30 ± 0,48), dok je za KS i KS-HA on ocenjen sa 3,00 ± 0,71. Posle 60 dana došlo je do opadanja znakova zapaljenja, koje je bilo najmanje izraženo oko KS-HA (0,20 ± 0,45). Najmanje izražena zapaljenska reakcija pulpnog tkiva kunića uočena je kod materijala KS (1,83 ± 0,75), zatim kod MTA i KS-HA (2,67 ± 1,53, 3,00 ± 0,63). Zaključak: Novosintetisani materijali su izazvali blagu zapaljensku reakciju potkožnog tkiva pacova, a KS-HA je pokazao najbolju tkivnu toleranciju. Nanostrukturni biomaterijali KS i KS-HA su uzrokovali blagu do umerenu zapaljensku reakciju pupnog tkiva kunića samo u neposrednoj blizini implantiranog materijala.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study
T1  - Histološke reakcije tkiva na novosintetisane bioaktivne materijale na bazi kalcijum-silikatnih sistema i hidroksiapatita – In vivo studija
VL  - 145
IS  - 7-8
SP  - 370
EP  - 377
DO  - 10.2298/SARH160719063O
ER  - 
@article{
author = "Opačić-Galić, Vanja and Petrović, Violeta and Jokanović, Vukoman and Živković, Slavoljub",
year = "2017",
abstract = "Introduction/Objective: Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatite-based (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods: The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results: Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ± 0.48), while CS and CS-HA scored 3 ± 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ± 0.45). The least visible inflammatory reaction of the rabbits’ pulp tissue was spotted with the CS (1.83 ± 0.75), than with the MTA and CS-HA (2.67 ± 1.53, 3 ± 0.63). Conclusion: The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits’ pulp tissue only in the immediate vicinity of the implanted material., Uvod/Cilj: Usavršavanje materijala koji bi mogli da se koriste kao biološke zamene kosti jedna je od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Cilj ovog rada je bio da se ispita odgovor tkiva na novosintetisane nanomaterijale na bazi kalcijum-silikatnih sistema (KS) i hidroksiapatita (KS-HA) posle implantacije u potkožno tkivo pacova i direktnog prekrivanja pulpe zuba kunića. Metode: U potkožno tkivo 40 vistar pacova su implantirani testirani materijali, a posle 7, 15, 30 i 60 dana životinje su žrtvovane. Direktno prekrivanje pulpe je realizovano na zubima kunića. Na vestibularnim površinama sekutića preparisani su kaviteti, a eksponirana pulpa je prekrivana testiranim materijalima. Životinje su žrtvovane posle 10 i 15 dana. Kontrolni materijal u oba eksperimenta je bio mineralni trioksidni agregat (MTA). Histološka analiza je obuhvatila praćenje ćelijske komponente zapaljenja, prisustva gigantskih ćelija i nekroze tkiva. Rezultati: Sedam dana posle supkutane implantacije najjači zapaljenski odgovor dao je MTA (3,30 ± 0,48), dok je za KS i KS-HA on ocenjen sa 3,00 ± 0,71. Posle 60 dana došlo je do opadanja znakova zapaljenja, koje je bilo najmanje izraženo oko KS-HA (0,20 ± 0,45). Najmanje izražena zapaljenska reakcija pulpnog tkiva kunića uočena je kod materijala KS (1,83 ± 0,75), zatim kod MTA i KS-HA (2,67 ± 1,53, 3,00 ± 0,63). Zaključak: Novosintetisani materijali su izazvali blagu zapaljensku reakciju potkožnog tkiva pacova, a KS-HA je pokazao najbolju tkivnu toleranciju. Nanostrukturni biomaterijali KS i KS-HA su uzrokovali blagu do umerenu zapaljensku reakciju pupnog tkiva kunića samo u neposrednoj blizini implantiranog materijala.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study, Histološke reakcije tkiva na novosintetisane bioaktivne materijale na bazi kalcijum-silikatnih sistema i hidroksiapatita – In vivo studija",
volume = "145",
number = "7-8",
pages = "370-377",
doi = "10.2298/SARH160719063O"
}
Opačić-Galić, V., Petrović, V., Jokanović, V.,& Živković, S.. (2017). Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 145(7-8), 370-377.
https://doi.org/10.2298/SARH160719063O
Opačić-Galić V, Petrović V, Jokanović V, Živković S. Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study. in Srpski arhiv za celokupno lekarstvo. 2017;145(7-8):370-377.
doi:10.2298/SARH160719063O .
Opačić-Galić, Vanja, Petrović, Violeta, Jokanović, Vukoman, Živković, Slavoljub, "Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study" in Srpski arhiv za celokupno lekarstvo, 145, no. 7-8 (2017):370-377,
https://doi.org/10.2298/SARH160719063O . .
7
4
5

Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite

Popović-Bajić, Marijana; Petrović, Violeta; Opačić-Galić, Vanja; Danilović, Vesna; Jokanović, Vukoman; Prokić, Branislav; Bolka-Prokić, Bogomir; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2016)

TY  - JOUR
AU  - Popović-Bajić, Marijana
AU  - Petrović, Violeta
AU  - Opačić-Galić, Vanja
AU  - Danilović, Vesna
AU  - Jokanović, Vukoman
AU  - Prokić, Branislav
AU  - Bolka-Prokić, Bogomir
AU  - Živković, Slavoljub
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2101
AB  - Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs.
AB  - Uvod Direktno prekrivanje pulpe je važan terapijski postupak koji ima zadatak da obezbedi zatvaranje pulpne komore i omogući proces zarastanja. Cilj ovog rada je bio da se ispita efekat novih nanostrukturnih materijala na bazi kalcijum-silikatnih sistema i hidroksiapatita na eksponiranu pulpu zuba vijetnamskih svinja. Materijal i metode rada Istraživanje je sprovedeno na 30 zuba kod dve vijetnamske svinje (Sus scrofa verus). Na vestibularnim površinama sekutića, očnjaka i prvih premolara urađene su preparacije kaviteta V klase, pri čemu je malim okruglim svrdlom napravljena namerna eksploracija komore pulpe. U prvoj eksperimentalnoj grupi (10 zuba) perforacija je prekrivana novim nanostrukturnim materijalom na bazi kalcijum-silikatnih sistema (CS). U drugoj eksperimentalnoj grupi (10 zuba) perforacija je prekrivana smešom kalcijum-silikatnog sistema i hidroksiapatita (HA-CS). U kontrolnoj grupi (10 zuba) perforacija je prekrivana sa MTA® (Dentsply Tulsa Dental, Džonson Siti, Tenesi, SAD). Svi kaviteti su restaurirani glas-jonomer cementom (GC Fuji VIII, GC Corporation, Tokio, Japan). Opservacioni period iznosio je 28 dana. Nakon žrtvovanja životinja napravljeni su histološki preparati na kojima su analizirani postojanje dentinskog mostića, inflamatorna reakcija pulpe, reorganizacija pulpnog tkiva i prisustvo bakterija. Rezultati Na svim zubima eksperimentalnih grupa i kontrolne grupe zabeleženo je stvaranje dentinskog mostića. Zapaljenje pulpe je bilo blago do umereno i u eksperimentalnim i u kontrolnoj grupi. Uočeni su znaci neoangiogeneze i mnoštvo ćelija sličnih odontoblastima koje su odgovorne za stvaranje dentinskog mostića. Nekroza nije zabeležena ni u jednom slučaju, kao ni prisustvo gram-pozitivnih bakterija u pulpi. Zaključak Histološka analiza je ukazala na povoljne terapijske efekte novih nanostrukturnih materijala na bazi kalcijum-silikatnih sistema i hidroksiapatita u direktnom prekrivanju pulpe zuba vijetnamskih svinja.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite
T1  - Direktno prekrivanje pulpe novim nanostrukturnim materijalima na bazi kalcijum-silikatnih sistema i hidroksiapatita
VL  - 63
IS  - 4
SP  - 183
EP  - 192
DO  - 10.1515/sdj-2016-0019
ER  - 
@article{
author = "Popović-Bajić, Marijana and Petrović, Violeta and Opačić-Galić, Vanja and Danilović, Vesna and Jokanović, Vukoman and Prokić, Branislav and Bolka-Prokić, Bogomir and Živković, Slavoljub",
year = "2016",
abstract = "Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs., Uvod Direktno prekrivanje pulpe je važan terapijski postupak koji ima zadatak da obezbedi zatvaranje pulpne komore i omogući proces zarastanja. Cilj ovog rada je bio da se ispita efekat novih nanostrukturnih materijala na bazi kalcijum-silikatnih sistema i hidroksiapatita na eksponiranu pulpu zuba vijetnamskih svinja. Materijal i metode rada Istraživanje je sprovedeno na 30 zuba kod dve vijetnamske svinje (Sus scrofa verus). Na vestibularnim površinama sekutića, očnjaka i prvih premolara urađene su preparacije kaviteta V klase, pri čemu je malim okruglim svrdlom napravljena namerna eksploracija komore pulpe. U prvoj eksperimentalnoj grupi (10 zuba) perforacija je prekrivana novim nanostrukturnim materijalom na bazi kalcijum-silikatnih sistema (CS). U drugoj eksperimentalnoj grupi (10 zuba) perforacija je prekrivana smešom kalcijum-silikatnog sistema i hidroksiapatita (HA-CS). U kontrolnoj grupi (10 zuba) perforacija je prekrivana sa MTA® (Dentsply Tulsa Dental, Džonson Siti, Tenesi, SAD). Svi kaviteti su restaurirani glas-jonomer cementom (GC Fuji VIII, GC Corporation, Tokio, Japan). Opservacioni period iznosio je 28 dana. Nakon žrtvovanja životinja napravljeni su histološki preparati na kojima su analizirani postojanje dentinskog mostića, inflamatorna reakcija pulpe, reorganizacija pulpnog tkiva i prisustvo bakterija. Rezultati Na svim zubima eksperimentalnih grupa i kontrolne grupe zabeleženo je stvaranje dentinskog mostića. Zapaljenje pulpe je bilo blago do umereno i u eksperimentalnim i u kontrolnoj grupi. Uočeni su znaci neoangiogeneze i mnoštvo ćelija sličnih odontoblastima koje su odgovorne za stvaranje dentinskog mostića. Nekroza nije zabeležena ni u jednom slučaju, kao ni prisustvo gram-pozitivnih bakterija u pulpi. Zaključak Histološka analiza je ukazala na povoljne terapijske efekte novih nanostrukturnih materijala na bazi kalcijum-silikatnih sistema i hidroksiapatita u direktnom prekrivanju pulpe zuba vijetnamskih svinja.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite, Direktno prekrivanje pulpe novim nanostrukturnim materijalima na bazi kalcijum-silikatnih sistema i hidroksiapatita",
volume = "63",
number = "4",
pages = "183-192",
doi = "10.1515/sdj-2016-0019"
}
Popović-Bajić, M., Petrović, V., Opačić-Galić, V., Danilović, V., Jokanović, V., Prokić, B., Bolka-Prokić, B.,& Živković, S.. (2016). Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(4), 183-192.
https://doi.org/10.1515/sdj-2016-0019
Popović-Bajić M, Petrović V, Opačić-Galić V, Danilović V, Jokanović V, Prokić B, Bolka-Prokić B, Živković S. Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite. in Stomatološki glasnik Srbije. 2016;63(4):183-192.
doi:10.1515/sdj-2016-0019 .
Popović-Bajić, Marijana, Petrović, Violeta, Opačić-Galić, Vanja, Danilović, Vesna, Jokanović, Vukoman, Prokić, Branislav, Bolka-Prokić, Bogomir, Živković, Slavoljub, "Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite" in Stomatološki glasnik Srbije, 63, no. 4 (2016):183-192,
https://doi.org/10.1515/sdj-2016-0019 . .
3

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

Petrović, V.; Opačić-Galić, Vanja; Živković, Slavoljub; Nikolić, B.; Danilović, Vesna; Miletić, Vesna; Jokanović, Vukoman; Mitić-Ćulafić, Dragana

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Petrović, V.
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
AU  - Nikolić, B.
AU  - Danilović, Vesna
AU  - Miletić, Vesna
AU  - Jokanović, Vukoman
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2518
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 
@article{
author = "Petrović, V. and Opačić-Galić, Vanja and Živković, Slavoljub and Nikolić, B. and Danilović, Vesna and Miletić, Vesna and Jokanović, Vukoman and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}
Petrović, V., Opačić-Galić, V., Živković, S., Nikolić, B., Danilović, V., Miletić, V., Jokanović, V.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal
Wiley, Hoboken., 48(10), 966-975.
https://doi.org/10.1111/iej.12391
Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V, Jokanović V, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .
Petrović, V., Opačić-Galić, Vanja, Živković, Slavoljub, Nikolić, B., Danilović, Vesna, Miletić, Vesna, Jokanović, Vukoman, Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .
22
12
15

Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite

Petrović, Violeta; Opačić-Galić, Vanja; Dželetović, Bojan; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2015)

TY  - JOUR
AU  - Petrović, Violeta
AU  - Opačić-Galić, Vanja
AU  - Dželetović, Bojan
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2068
AB  - Introduction Calcium silicate cements can be successfully used for the treatment of root perforations due to their exceptional biological and sealing properties. The aim of this study was to test, using dye penetration method, marginal microleakage of newly synthesized nanostructured biomaterials based on calcium silicate system and hydroxyapatite after their application in interradicular perforation of extracted teeth. Material and Methods The study included 34 extracted human molars. Newly synthesized nanostructured materials: one based on calcium silicate system (CS), and the other one based on hydroxyapatite and active calcium silicate system (HA-CS) were tested. Mineral trioxide agreaggate (MTA; Angelus, Londrina, Brazil) was used as control. Marginal microleakage was evaluated using dye penetration test 6 months after the application of materials in experimentally prepared inter-radicular perforations in extracted human molars. Dye penetration was analyzed using light microscope at 30X magnification, a method of quantifying visual information in computer software for image processing (Adobe Photoshop CS5 Extended, version 12.0 x 32). The values were expressed in millimeters, and the results statistically analyzed using one-way ANOVA and Tukey post-hoc test (α=0.05). Results The shortest dye penetration was measured for calcium silicate system (0.44 mm), while slightly higher values were found for MTA (0.54 mm). Dye penetration for hydroxyapatite and active calcium silicate system (2.00 mm) was longer than for the other two materials (p lt 0.05). Conclusion The lowest marginal microleakage was observed in CS and was comparable to MTA. Microleakage in HA-CS was significantly higher than CS and MTA.
AB  - Uvod Primena cemenata od kalcijum-silikata u lečenju perforacija korena zuba zasniva se na njihovim izuzetnim biološkim svojstvima i odgovarajućem rubnom zaptivanju. Cilj ovog rada bio je da se testom prodora boje ispita marginalna mikropropustljivost novosintetisanih nanostrukturnih biomaterijala na bazi kalcijumsilikatnih sistema i hidroksiapatita nakon primene materijala u interradiksne perforacije ekstrahovanih zuba. Materijal i metode rada Ispitivanje je realizovano na 34 ekstrahovana humana molara. Testirani su novosintetisani nanostrukturni materijali: materijal na bazi aktivnih kalcijumsilikatnih sistema (CS) i materijal na bazi hidroksiapatita i aktivnih kalcijumsilikatnih sistema (HA-CS). Kao kontrolni materijal korišćen je komercijalni kalcijumsilikatni cement (MTA Angelus, Londrina, Brazil). Marginalna mikropropustljivost je ispitivana testom prodora boje šest meseci nakon primene materijala u eksperimentalno preparisane interradiksne perforacije na ekstrahovanim humanim molarima. Prodor boje je analiziran svetlosnim mikroskopom na uveličanju od 30 puta metodom kvantifikovanja vizuelnih informacija kompjuterskim programom za obradu slike (Adobe Photoshop CS5 Extended, verzija 12.0x32). Dobijene vrednosti su izražene u milimetrima, a dobijeni rezultati statistički su obrađeni primenom jednofaktorskog testa ANOVA sa Tukijevim (Tukey) post-hoc testom (α=0,05). Rezultati Najmanji prodor boje izmeren je kod materijala CS (0,44 mm), dok su nešto veće vrednosti zabeležene kod MTA (0,54 mm). Prodor boje kod materijala HA-CS (2,00 mm) bio je značajno veći u odnosu na materijale CS i MTA (p lt 0,05). Zaključak Najmanja marginalna mikropropustljivost zabeležena je kod materijala CS i bila je komparabilna sa MTA. Mikropropustljivost materijala HA-CS bila je značajno veća u odnosu na materijal CS i kontrolni materijal MTA.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite
T1  - Ispitivanje marginalne mikropropustljivosti novosintetisanih nanostrukturnih biomaterijala na bazi aktivnih kalcijum silikatnih sistema i hidroksiapatita
VL  - 62
IS  - 3
SP  - 109
EP  - 116
DO  - 10.1515/sdj-2015-0011
ER  - 
@article{
author = "Petrović, Violeta and Opačić-Galić, Vanja and Dželetović, Bojan and Jokanović, Vukoman and Živković, Slavoljub",
year = "2015",
abstract = "Introduction Calcium silicate cements can be successfully used for the treatment of root perforations due to their exceptional biological and sealing properties. The aim of this study was to test, using dye penetration method, marginal microleakage of newly synthesized nanostructured biomaterials based on calcium silicate system and hydroxyapatite after their application in interradicular perforation of extracted teeth. Material and Methods The study included 34 extracted human molars. Newly synthesized nanostructured materials: one based on calcium silicate system (CS), and the other one based on hydroxyapatite and active calcium silicate system (HA-CS) were tested. Mineral trioxide agreaggate (MTA; Angelus, Londrina, Brazil) was used as control. Marginal microleakage was evaluated using dye penetration test 6 months after the application of materials in experimentally prepared inter-radicular perforations in extracted human molars. Dye penetration was analyzed using light microscope at 30X magnification, a method of quantifying visual information in computer software for image processing (Adobe Photoshop CS5 Extended, version 12.0 x 32). The values were expressed in millimeters, and the results statistically analyzed using one-way ANOVA and Tukey post-hoc test (α=0.05). Results The shortest dye penetration was measured for calcium silicate system (0.44 mm), while slightly higher values were found for MTA (0.54 mm). Dye penetration for hydroxyapatite and active calcium silicate system (2.00 mm) was longer than for the other two materials (p lt 0.05). Conclusion The lowest marginal microleakage was observed in CS and was comparable to MTA. Microleakage in HA-CS was significantly higher than CS and MTA., Uvod Primena cemenata od kalcijum-silikata u lečenju perforacija korena zuba zasniva se na njihovim izuzetnim biološkim svojstvima i odgovarajućem rubnom zaptivanju. Cilj ovog rada bio je da se testom prodora boje ispita marginalna mikropropustljivost novosintetisanih nanostrukturnih biomaterijala na bazi kalcijumsilikatnih sistema i hidroksiapatita nakon primene materijala u interradiksne perforacije ekstrahovanih zuba. Materijal i metode rada Ispitivanje je realizovano na 34 ekstrahovana humana molara. Testirani su novosintetisani nanostrukturni materijali: materijal na bazi aktivnih kalcijumsilikatnih sistema (CS) i materijal na bazi hidroksiapatita i aktivnih kalcijumsilikatnih sistema (HA-CS). Kao kontrolni materijal korišćen je komercijalni kalcijumsilikatni cement (MTA Angelus, Londrina, Brazil). Marginalna mikropropustljivost je ispitivana testom prodora boje šest meseci nakon primene materijala u eksperimentalno preparisane interradiksne perforacije na ekstrahovanim humanim molarima. Prodor boje je analiziran svetlosnim mikroskopom na uveličanju od 30 puta metodom kvantifikovanja vizuelnih informacija kompjuterskim programom za obradu slike (Adobe Photoshop CS5 Extended, verzija 12.0x32). Dobijene vrednosti su izražene u milimetrima, a dobijeni rezultati statistički su obrađeni primenom jednofaktorskog testa ANOVA sa Tukijevim (Tukey) post-hoc testom (α=0,05). Rezultati Najmanji prodor boje izmeren je kod materijala CS (0,44 mm), dok su nešto veće vrednosti zabeležene kod MTA (0,54 mm). Prodor boje kod materijala HA-CS (2,00 mm) bio je značajno veći u odnosu na materijale CS i MTA (p lt 0,05). Zaključak Najmanja marginalna mikropropustljivost zabeležena je kod materijala CS i bila je komparabilna sa MTA. Mikropropustljivost materijala HA-CS bila je značajno veća u odnosu na materijal CS i kontrolni materijal MTA.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite, Ispitivanje marginalne mikropropustljivosti novosintetisanih nanostrukturnih biomaterijala na bazi aktivnih kalcijum silikatnih sistema i hidroksiapatita",
volume = "62",
number = "3",
pages = "109-116",
doi = "10.1515/sdj-2015-0011"
}
Petrović, V., Opačić-Galić, V., Dželetović, B., Jokanović, V.,& Živković, S.. (2015). Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(3), 109-116.
https://doi.org/10.1515/sdj-2015-0011
Petrović V, Opačić-Galić V, Dželetović B, Jokanović V, Živković S. Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite. in Stomatološki glasnik Srbije. 2015;62(3):109-116.
doi:10.1515/sdj-2015-0011 .
Petrović, Violeta, Opačić-Galić, Vanja, Dželetović, Bojan, Jokanović, Vukoman, Živković, Slavoljub, "Marginal microleakage of newly synthesized nanostructured biomaterials based on active calcium silicate systems and hydroxyapatite" in Stomatološki glasnik Srbije, 62, no. 3 (2015):109-116,
https://doi.org/10.1515/sdj-2015-0011 . .

Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study

Petrović, V.; Opačić-Galić, Vanja; Živković, Slavoljub; Nikolić, B.; Danilović, Vesna; Miletić, Vesna; Jokanović, Vukoman; Mitić-Ćulafić, Dragana

(Wiley, Hoboken, 2015)

TY  - JOUR
AU  - Petrović, V.
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
AU  - Nikolić, B.
AU  - Danilović, Vesna
AU  - Miletić, Vesna
AU  - Jokanović, Vukoman
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2012
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 
@article{
author = "Petrović, V. and Opačić-Galić, Vanja and Živković, Slavoljub and Nikolić, B. and Danilović, Vesna and Miletić, Vesna and Jokanović, Vukoman and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukey's post hoc test as well as Kruskall-Wallis test with Dunn's post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P lt 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P lt 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P>0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}
Petrović, V., Opačić-Galić, V., Živković, S., Nikolić, B., Danilović, V., Miletić, V., Jokanović, V.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal
Wiley, Hoboken., 48(10), 966-975.
https://doi.org/10.1111/iej.12391
Petrović V, Opačić-Galić V, Živković S, Nikolić B, Danilović V, Miletić V, Jokanović V, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .
Petrović, V., Opačić-Galić, Vanja, Živković, Slavoljub, Nikolić, B., Danilović, Vesna, Miletić, Vesna, Jokanović, Vukoman, Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .
22
12
15

Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite

Opačić-Galić, Vanja

(Univerzitet u Beogradu, Stomatološki fakultet, 2014)

TY  - THES
AU  - Opačić-Galić, Vanja
PY  - 2014
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2832
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:11033/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47571215
UR  - http://nardus.mpn.gov.rs/123456789/5270
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/974
AB  - Development of new materials, that could be used as biological bone "replacement", is today one of the most important and the most active research areas of biomaterials. Bioactivity and biocompatibility of these materials provides the interaction with biological systems, and thereby enables restoring the function of damaged tissues and/or organs in the human body. In recent years, a large number of materials for endodontic applications have been developed. Besides biocompatibility, these materials have an additional role as bio- inducers in health preservation of the periodontal and pulpal tissue, as well as stimulation of damaged tissues regeneration. The aim of this study was to examine the biocompatibility and bioactivity of new nanostructured materials based on calcium silicate systems (CS) and hydroxyapatites (CS-HA). The first step in this research was to analyze the composition, particle sizing, and to determine the setting time and the strength of the newly synthesized material. The structure of calcium silicates is mainly composed of agglomerates with few micrometers in size, which are made of smaller particles with sizes between 117 and 477nm. These particles are generally spherical or ellipsoidal shape, more or less elongated in one direction. HA particles were irregular in shape, mostly rounded, with dimensions of 60 nm to 470nm. EDS analysis showed that the chemical composition of calcium silicate (Ca 22.21 , 8.22 Si , O 69.7 in atomic % ) corresponded to the Ca/Si ratio in about 2.7 (in atomic %). The setting time of the CS began 3 minutes after addition of deionized water, and the bonding was completed within 10 minutes after. For HA-CS these values were 5 and 15 minutes, respectively. To test the biocompatibility of these materials pre-clinical in vitro and in vivo assays were used...
AB  - Usavršavanje materijala koji bi mogli da se koriste kao biološke «zamene» kosti,danas je jedna od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Biokompatibilnost i bioaktivnost ovih materijala obezbeđuje interakciju sa biološkim sistemima, a time i obnavljanje funkcije oštećenih tkiva i/ili organa u ljudskom organizmu... Poslednjih godina razvijen je veliki broj materijala za endodontsku primenu, koji osim biokompatibilnosti imaju ulogu i bio-induktora u očuvanju zdravlja pulpe i periodontalnog tkiva, ali i stimulišu regeneraciju oštećenih tkiva. Cilj ovog istraživanja je bio da se proveri biokompatibilnost i biofunkcionalnost novih nanostrukturnih materijala na bazi kalcijum silikatnih sistema (CS) i hidroksiapatita (CS-HA). Prvi korak u ovom istraživanju je bilo analiziranje sastava, određivanje veličine čestica, merenje vremena vezivanja i određivanje čvrstoće novosintetisanih materijala. Strukturu kalcijum silikata čine uglavnom aglomerati veličine nekoliko mikrometara koji su izgrađeni od manjih partikula (čestica) sa dimenzijama između 117 i 477nm. Ove partikule su uglavnom sferičnog ili elipsoidnog oblika, manje ili više izdužene u jednom pravcu. Partikule HA su bile nepravilnog oblika, većinom okruglaste, sa dimenzijama od 60 nm do 470nm. EDS analiza je pokazala da je hemijski sastav kalcijum silikata (Ca 22.21, Si 8.22, O 69.7 u atomskim%) odgovarao odnosu Ca/Si od približno 2.7 (atomski%). Vreme vezivanja za CS je započinjalo 3 minuta nakon dodavanja dejonizovane vode, a kompletno vezivanje se završava nakon 10 minuta. Za CSHA ove vrednosti su bile 5 i 15 minuta, respektivno. Za proveru biokompatibilnosti ovih materijala korišćeni su predklinički in vitro i in vivo testovi.
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite
T1  - Ispitivanje biokompatibilnosti nanostrukturnih materijala na bazi aktivnih kalcijum silikatnih sistema i hidroksiapatita
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5270
ER  - 
@phdthesis{
author = "Opačić-Galić, Vanja",
year = "2014",
abstract = "Development of new materials, that could be used as biological bone "replacement", is today one of the most important and the most active research areas of biomaterials. Bioactivity and biocompatibility of these materials provides the interaction with biological systems, and thereby enables restoring the function of damaged tissues and/or organs in the human body. In recent years, a large number of materials for endodontic applications have been developed. Besides biocompatibility, these materials have an additional role as bio- inducers in health preservation of the periodontal and pulpal tissue, as well as stimulation of damaged tissues regeneration. The aim of this study was to examine the biocompatibility and bioactivity of new nanostructured materials based on calcium silicate systems (CS) and hydroxyapatites (CS-HA). The first step in this research was to analyze the composition, particle sizing, and to determine the setting time and the strength of the newly synthesized material. The structure of calcium silicates is mainly composed of agglomerates with few micrometers in size, which are made of smaller particles with sizes between 117 and 477nm. These particles are generally spherical or ellipsoidal shape, more or less elongated in one direction. HA particles were irregular in shape, mostly rounded, with dimensions of 60 nm to 470nm. EDS analysis showed that the chemical composition of calcium silicate (Ca 22.21 , 8.22 Si , O 69.7 in atomic % ) corresponded to the Ca/Si ratio in about 2.7 (in atomic %). The setting time of the CS began 3 minutes after addition of deionized water, and the bonding was completed within 10 minutes after. For HA-CS these values were 5 and 15 minutes, respectively. To test the biocompatibility of these materials pre-clinical in vitro and in vivo assays were used..., Usavršavanje materijala koji bi mogli da se koriste kao biološke «zamene» kosti,danas je jedna od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Biokompatibilnost i bioaktivnost ovih materijala obezbeđuje interakciju sa biološkim sistemima, a time i obnavljanje funkcije oštećenih tkiva i/ili organa u ljudskom organizmu... Poslednjih godina razvijen je veliki broj materijala za endodontsku primenu, koji osim biokompatibilnosti imaju ulogu i bio-induktora u očuvanju zdravlja pulpe i periodontalnog tkiva, ali i stimulišu regeneraciju oštećenih tkiva. Cilj ovog istraživanja je bio da se proveri biokompatibilnost i biofunkcionalnost novih nanostrukturnih materijala na bazi kalcijum silikatnih sistema (CS) i hidroksiapatita (CS-HA). Prvi korak u ovom istraživanju je bilo analiziranje sastava, određivanje veličine čestica, merenje vremena vezivanja i određivanje čvrstoće novosintetisanih materijala. Strukturu kalcijum silikata čine uglavnom aglomerati veličine nekoliko mikrometara koji su izgrađeni od manjih partikula (čestica) sa dimenzijama između 117 i 477nm. Ove partikule su uglavnom sferičnog ili elipsoidnog oblika, manje ili više izdužene u jednom pravcu. Partikule HA su bile nepravilnog oblika, većinom okruglaste, sa dimenzijama od 60 nm do 470nm. EDS analiza je pokazala da je hemijski sastav kalcijum silikata (Ca 22.21, Si 8.22, O 69.7 u atomskim%) odgovarao odnosu Ca/Si od približno 2.7 (atomski%). Vreme vezivanja za CS je započinjalo 3 minuta nakon dodavanja dejonizovane vode, a kompletno vezivanje se završava nakon 10 minuta. Za CSHA ove vrednosti su bile 5 i 15 minuta, respektivno. Za proveru biokompatibilnosti ovih materijala korišćeni su predklinički in vitro i in vivo testovi.",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite, Ispitivanje biokompatibilnosti nanostrukturnih materijala na bazi aktivnih kalcijum silikatnih sistema i hidroksiapatita",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5270"
}
Opačić-Galić, V.. (2014). Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_5270
Opačić-Galić V. Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. 2014;.
https://hdl.handle.net/21.15107/rcub_nardus_5270 .
Opačić-Galić, Vanja, "Biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite" (2014),
https://hdl.handle.net/21.15107/rcub_nardus_5270 .

New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes

Opačić-Galić, Vanja; Petrović, V.; Živković, Slavoljub; Jokanović, Vukoman; Nikolić, B.; Knežević-Vukcević, Jelena; Mitić-Ćulafić, Dragana

(Wiley, Hoboken, 2013)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Petrović, V.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
AU  - Nikolić, B.
AU  - Knežević-Vukcević, Jelena
AU  - Mitić-Ćulafić, Dragana
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1838
AB  - Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes
VL  - 46
IS  - 6
SP  - 506
EP  - 516
DO  - 10.1111/iej.12017
ER  - 
@article{
author = "Opačić-Galić, Vanja and Petrović, V. and Živković, Slavoljub and Jokanović, Vukoman and Nikolić, B. and Knežević-Vukcević, Jelena and Mitić-Ćulafić, Dragana",
year = "2013",
abstract = "Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes",
volume = "46",
number = "6",
pages = "506-516",
doi = "10.1111/iej.12017"
}
Opačić-Galić, V., Petrović, V., Živković, S., Jokanović, V., Nikolić, B., Knežević-Vukcević, J.,& Mitić-Ćulafić, D.. (2013). New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal
Wiley, Hoboken., 46(6), 506-516.
https://doi.org/10.1111/iej.12017
Opačić-Galić V, Petrović V, Živković S, Jokanović V, Nikolić B, Knežević-Vukcević J, Mitić-Ćulafić D. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal. 2013;46(6):506-516.
doi:10.1111/iej.12017 .
Opačić-Galić, Vanja, Petrović, V., Živković, Slavoljub, Jokanović, Vukoman, Nikolić, B., Knežević-Vukcević, Jelena, Mitić-Ćulafić, Dragana, "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes" in International Endodontic Journal, 46, no. 6 (2013):506-516,
https://doi.org/10.1111/iej.12017 . .
2
40
29
36

Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats

Petrović, Violeta; Opačić-Galić, Vanja; Jokanović, Vukoman; Jovanović, M.; Basta-Jovanović, Gordana; Živković, Slavoljub

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2012)

TY  - JOUR
AU  - Petrović, Violeta
AU  - Opačić-Galić, Vanja
AU  - Jokanović, Vukoman
AU  - Jovanović, M.
AU  - Basta-Jovanović, Gordana
AU  - Živković, Slavoljub
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1708
AB  - The aim of the study was to investigate rat connective tissue response to a new calcium silicate system 7, 15, 30 and 60 days after implantation. Twenty Wistar albino male rats received two tubes half-filled with a new calcium silicate system (NCSS) or MTA in subcutaneous tissue. The empty half of the tubes served as controls. Five animals were sacrificed after 7, 15, 30 and 60 days and samples of the subcutaneous tissue around implanted material were submitted to histological analysis. The intensity of inflammation was evaluated based on the number of inflammatory cells present. Statistical analysis was performed using one way ANOVA and Holm Sidak's multiple comparison tests. Mild to moderate inflammatory reaction was observed after 7, 15 and 30 days around a NCSS while mild inflammatory reaction was detected after 60 days of implantation. In the MTA group, mild to moderate inflammatory reaction was found after 7 and 15 days while mild inflammatory reaction was present after 30 and 60 days. There was no statistically significant difference in the intensity of inflammatory reactions between the tested materials and control groups in any experimental period (ANOVA p>0.05). Regarding the intensity of inflammatory reactions at different experimental periods, a statistically significant difference was observed between 7 and 30 days, 7 and 60 days and 15 to 60 days for both materials. For the controls, a statistically significant difference was found between 7 and 60 days and 15 and 60 days of the experiment (Holm Sidak  lt  p 0.001). Subcutaneous tissue of rats showed good tolerance to a new calcium silicate system. Inflammatory reaction was similar to that caused by MTA.
AB  - Cilj ovog rada je bio da se ispita biokompatibilnost novog kalcijum silikatnog sistema nakon in vivo implantacije u potkožno tkivo pacova. Istraživanje je obuhvatilo 20 Wistar albino pacova muškog pola. U svaku životinju implantirane su dve tube do pola ispunjene novim kalcijum silikatnim sistemom (NCSS) odnosno MTA-om. Prazne polovine tuba služile su kao kontrola. Po 5 životinja žrtvovano je nakon 7, 15, 30 i 60 dana, nakon čega su uzorci potkožnog tkiva oko implantiranog materijala pripremljeni za histološku analizu. Intezitet zapaljenske reakcije je procenjivan na osnovu broja prisutnih ćelija zapaljenja. Statistička analiza je urađena ANOVA testom i Holm Sidak-ovim testom višestruke komparacije. U eksperimentalnim periodima 7, 15 i 30 dana u NCSS grupi je uočena blaga do umerena zapaljenska reakcija, a nakon 60 dana samo blaga zapaljenska reakcija. UMTA grupi, nakon 7 i 15 dana uočena je blaga do umerena zapaljenska reakcija, a nakon 30 i 60 dana blaga zapaljenska reakcija. Nije bilo statistički značajne razlike u intezitetu zapaljenske reakcije između testiranih materijala i kontrolne grupe ni u jednom eksperimentalnom periodu (ANOVA p>0.05). Poređenjem inteziteta zapaljenskih reakcija u različitim eksperimentalnim periodima, uočeno je postojanje statistički značajnih razlika kod oba testirana materijala između 7 i 30 dana, 7 i 60 dana kao i između 15 i 60 dana, a kod kontrole između 7 i 60 dana i 15 i 60 dana eksperimenta (Holm Sidak p  lt  0.001). Novi kalcijum silikatni sistem je pokazao biokompatibilno ponašanje. Inflamatorne reakcije potkožnog tkiva bile su slične onima koje je izazvao MTA.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats
T1  - Ispitivanje biokompatibilnosti novog nanostrukturalnog materijala na bazi kalcijum silikatnih sistema implantiranjem u potkožno tkivo pacova
VL  - 62
IS  - 5-6
SP  - 697
EP  - 708
DO  - 10.2298/AVB1206697P
ER  - 
@article{
author = "Petrović, Violeta and Opačić-Galić, Vanja and Jokanović, Vukoman and Jovanović, M. and Basta-Jovanović, Gordana and Živković, Slavoljub",
year = "2012",
abstract = "The aim of the study was to investigate rat connective tissue response to a new calcium silicate system 7, 15, 30 and 60 days after implantation. Twenty Wistar albino male rats received two tubes half-filled with a new calcium silicate system (NCSS) or MTA in subcutaneous tissue. The empty half of the tubes served as controls. Five animals were sacrificed after 7, 15, 30 and 60 days and samples of the subcutaneous tissue around implanted material were submitted to histological analysis. The intensity of inflammation was evaluated based on the number of inflammatory cells present. Statistical analysis was performed using one way ANOVA and Holm Sidak's multiple comparison tests. Mild to moderate inflammatory reaction was observed after 7, 15 and 30 days around a NCSS while mild inflammatory reaction was detected after 60 days of implantation. In the MTA group, mild to moderate inflammatory reaction was found after 7 and 15 days while mild inflammatory reaction was present after 30 and 60 days. There was no statistically significant difference in the intensity of inflammatory reactions between the tested materials and control groups in any experimental period (ANOVA p>0.05). Regarding the intensity of inflammatory reactions at different experimental periods, a statistically significant difference was observed between 7 and 30 days, 7 and 60 days and 15 to 60 days for both materials. For the controls, a statistically significant difference was found between 7 and 60 days and 15 and 60 days of the experiment (Holm Sidak  lt  p 0.001). Subcutaneous tissue of rats showed good tolerance to a new calcium silicate system. Inflammatory reaction was similar to that caused by MTA., Cilj ovog rada je bio da se ispita biokompatibilnost novog kalcijum silikatnog sistema nakon in vivo implantacije u potkožno tkivo pacova. Istraživanje je obuhvatilo 20 Wistar albino pacova muškog pola. U svaku životinju implantirane su dve tube do pola ispunjene novim kalcijum silikatnim sistemom (NCSS) odnosno MTA-om. Prazne polovine tuba služile su kao kontrola. Po 5 životinja žrtvovano je nakon 7, 15, 30 i 60 dana, nakon čega su uzorci potkožnog tkiva oko implantiranog materijala pripremljeni za histološku analizu. Intezitet zapaljenske reakcije je procenjivan na osnovu broja prisutnih ćelija zapaljenja. Statistička analiza je urađena ANOVA testom i Holm Sidak-ovim testom višestruke komparacije. U eksperimentalnim periodima 7, 15 i 30 dana u NCSS grupi je uočena blaga do umerena zapaljenska reakcija, a nakon 60 dana samo blaga zapaljenska reakcija. UMTA grupi, nakon 7 i 15 dana uočena je blaga do umerena zapaljenska reakcija, a nakon 30 i 60 dana blaga zapaljenska reakcija. Nije bilo statistički značajne razlike u intezitetu zapaljenske reakcije između testiranih materijala i kontrolne grupe ni u jednom eksperimentalnom periodu (ANOVA p>0.05). Poređenjem inteziteta zapaljenskih reakcija u različitim eksperimentalnim periodima, uočeno je postojanje statistički značajnih razlika kod oba testirana materijala između 7 i 30 dana, 7 i 60 dana kao i između 15 i 60 dana, a kod kontrole između 7 i 60 dana i 15 i 60 dana eksperimenta (Holm Sidak p  lt  0.001). Novi kalcijum silikatni sistem je pokazao biokompatibilno ponašanje. Inflamatorne reakcije potkožnog tkiva bile su slične onima koje je izazvao MTA.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats, Ispitivanje biokompatibilnosti novog nanostrukturalnog materijala na bazi kalcijum silikatnih sistema implantiranjem u potkožno tkivo pacova",
volume = "62",
number = "5-6",
pages = "697-708",
doi = "10.2298/AVB1206697P"
}
Petrović, V., Opačić-Galić, V., Jokanović, V., Jovanović, M., Basta-Jovanović, G.,& Živković, S.. (2012). Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 697-708.
https://doi.org/10.2298/AVB1206697P
Petrović V, Opačić-Galić V, Jokanović V, Jovanović M, Basta-Jovanović G, Živković S. Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats. in Acta veterinaria. 2012;62(5-6):697-708.
doi:10.2298/AVB1206697P .
Petrović, Violeta, Opačić-Galić, Vanja, Jokanović, Vukoman, Jovanović, M., Basta-Jovanović, Gordana, Živković, Slavoljub, "Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats" in Acta veterinaria, 62, no. 5-6 (2012):697-708,
https://doi.org/10.2298/AVB1206697P . .
7
6
6

Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth

Petrović, Violeta; Opačić-Galić, Vanja; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2011)

TY  - JOUR
AU  - Petrović, Violeta
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1671
AB  - Introduction. Postoperative pain may develop after chemomechanical preparation during endodontic treatment or retreatment. It is associated with acute apical periodontitis caused by bacteria penetrated from the root canal into the periapical tissue. The purpose of this study was to evaluate the incidence of postoperative pain during endodontic treatment after intracanal medication performed with calcium hydroxide (CH) paste or 1% chlorhexidine gel (CHX). Material and Methods. The study included 22 asymptomatic teeth in both male and female patients. Fourteen teeth had the diagnosis of pulp necrosis and the other eight were retreatment cases. The root canals were prepared by crown-down technique using K files and copious irrigation with 0.5% sodium hypochlorite (NaOCl). Intracanal dressing was performed using either calcium hydroxide paste or 1% CHX gel. Each medicament was placed in the root canals of eleven randomly selected teeth. The teeth were restored with temporary filling. Postoperative pain was registered during the seven day period between two appointments. The level of pain was rated as follows: no pain, mild pain, moderate pain and severe pain (flare-up). The obtained data was analyzed using Fisher exact test. The level of significance was α=0.05. Results. Postoperative pain was absent in 77.3% of total number of treated teeth. Severe pain (flare-up) was registered in 2 cases (9%). There was no significant difference in pain incidence between the CH paste and CHX gel group (p=0.610). Conclusion. Endodontic procedure used in this study which considered root canal instrumentation and irrigation followed by intracanal medication with CH and CHX resulted in low incidence of postoperative pain.
AB  - Uvod. Postoperacioni bol je najčešća komplikacija hemomehaničke preparacije kanala korena zuba, a posledica je akutnog zapaljenja u periradikularnom tkivu nastalog usled prodora nadražaja iz kanala korena, najčešće mikroorganizama. Cilj rada je bio da se ispita učestalost bola nakon endodontskog lečenja i ponovnog tretmana asimptomatskih zuba posle medikacije kanala korena pastom kalcijum-hidroksida (CH), odnosno gelom jednoprocentnog hlorheksidina (CHX). Materijal i metode rada. Tokom istraživanja ispitana su 22 asimptomatska zuba. Kod 14 zuba je dijagnostikovana nekroza pulpe, dok je kod osam zuba bilo potrebno uraditi ponovni tretman. Preparacija kanala korena je urađena crown-down tehnikom i K-turpijama uz obilnu irigaciju rastvorom NaOCl od 0,5%. Zubi su nasumično podeljeni u dve grupe od po 11 zuba. U prvoj grupi kanali korena su posle instrumentacije napunjeni CH pastom, a u drugoj grupi je za medikaciju kanala korišćen CHX gel. Nakon toga zubi su privremeno zatvoreni, a pojava bola je praćena do naredne posete posle sedam dana. Intenzitet bola je rangiran po sledećoj skali: bez bola, blag bol, umeren bol i jak (neizdržljiv) bol. Dobijeni podaci su statistički obrađeni primenom Fišerovog testa na nivou značajnosti od α=0,05. Rezultati. U 17 zuba (77,3%) nije bilo postoperacionog bola. Među pacijentima koji su osećali bolove jak bol je zabeležen u dva slučaja (9%). Nisu uočene statistički značajne razlike u učestalosti bola u zavisnosti od korišćenog intrakanalnog medikamenta (p=0,610). Zaključak. Endodontski postupci primenjeni u ovom istraživanju, zasnovani na temeljnoj instrumentaciji uz irigaciju rastvorom NaOCl i interseansnoj medikaciji kanala CH pastom, odnosno CHX gelom, doveli su do male incidencije postoperacionog bola.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth
T1  - Učestalost bola posle endodontskog lečenja i ponovnog tretmana asimptomatskih zuba
VL  - 58
IS  - 2
SP  - 75
EP  - 81
DO  - 10.2298/SGS1102075P
ER  - 
@article{
author = "Petrović, Violeta and Opačić-Galić, Vanja and Živković, Slavoljub",
year = "2011",
abstract = "Introduction. Postoperative pain may develop after chemomechanical preparation during endodontic treatment or retreatment. It is associated with acute apical periodontitis caused by bacteria penetrated from the root canal into the periapical tissue. The purpose of this study was to evaluate the incidence of postoperative pain during endodontic treatment after intracanal medication performed with calcium hydroxide (CH) paste or 1% chlorhexidine gel (CHX). Material and Methods. The study included 22 asymptomatic teeth in both male and female patients. Fourteen teeth had the diagnosis of pulp necrosis and the other eight were retreatment cases. The root canals were prepared by crown-down technique using K files and copious irrigation with 0.5% sodium hypochlorite (NaOCl). Intracanal dressing was performed using either calcium hydroxide paste or 1% CHX gel. Each medicament was placed in the root canals of eleven randomly selected teeth. The teeth were restored with temporary filling. Postoperative pain was registered during the seven day period between two appointments. The level of pain was rated as follows: no pain, mild pain, moderate pain and severe pain (flare-up). The obtained data was analyzed using Fisher exact test. The level of significance was α=0.05. Results. Postoperative pain was absent in 77.3% of total number of treated teeth. Severe pain (flare-up) was registered in 2 cases (9%). There was no significant difference in pain incidence between the CH paste and CHX gel group (p=0.610). Conclusion. Endodontic procedure used in this study which considered root canal instrumentation and irrigation followed by intracanal medication with CH and CHX resulted in low incidence of postoperative pain., Uvod. Postoperacioni bol je najčešća komplikacija hemomehaničke preparacije kanala korena zuba, a posledica je akutnog zapaljenja u periradikularnom tkivu nastalog usled prodora nadražaja iz kanala korena, najčešće mikroorganizama. Cilj rada je bio da se ispita učestalost bola nakon endodontskog lečenja i ponovnog tretmana asimptomatskih zuba posle medikacije kanala korena pastom kalcijum-hidroksida (CH), odnosno gelom jednoprocentnog hlorheksidina (CHX). Materijal i metode rada. Tokom istraživanja ispitana su 22 asimptomatska zuba. Kod 14 zuba je dijagnostikovana nekroza pulpe, dok je kod osam zuba bilo potrebno uraditi ponovni tretman. Preparacija kanala korena je urađena crown-down tehnikom i K-turpijama uz obilnu irigaciju rastvorom NaOCl od 0,5%. Zubi su nasumično podeljeni u dve grupe od po 11 zuba. U prvoj grupi kanali korena su posle instrumentacije napunjeni CH pastom, a u drugoj grupi je za medikaciju kanala korišćen CHX gel. Nakon toga zubi su privremeno zatvoreni, a pojava bola je praćena do naredne posete posle sedam dana. Intenzitet bola je rangiran po sledećoj skali: bez bola, blag bol, umeren bol i jak (neizdržljiv) bol. Dobijeni podaci su statistički obrađeni primenom Fišerovog testa na nivou značajnosti od α=0,05. Rezultati. U 17 zuba (77,3%) nije bilo postoperacionog bola. Među pacijentima koji su osećali bolove jak bol je zabeležen u dva slučaja (9%). Nisu uočene statistički značajne razlike u učestalosti bola u zavisnosti od korišćenog intrakanalnog medikamenta (p=0,610). Zaključak. Endodontski postupci primenjeni u ovom istraživanju, zasnovani na temeljnoj instrumentaciji uz irigaciju rastvorom NaOCl i interseansnoj medikaciji kanala CH pastom, odnosno CHX gelom, doveli su do male incidencije postoperacionog bola.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth, Učestalost bola posle endodontskog lečenja i ponovnog tretmana asimptomatskih zuba",
volume = "58",
number = "2",
pages = "75-81",
doi = "10.2298/SGS1102075P"
}
Petrović, V., Opačić-Galić, V.,& Živković, S.. (2011). Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 58(2), 75-81.
https://doi.org/10.2298/SGS1102075P
Petrović V, Opačić-Galić V, Živković S. Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth. in Stomatološki glasnik Srbije. 2011;58(2):75-81.
doi:10.2298/SGS1102075P .
Petrović, Violeta, Opačić-Galić, Vanja, Živković, Slavoljub, "Postoperative pain after primary endodontic treatment and retreatment of asymptomatic teeth" in Stomatološki glasnik Srbije, 58, no. 2 (2011):75-81,
https://doi.org/10.2298/SGS1102075P . .
4

Endodontic therapy of pathological resorptions of tooth root

Opačić-Galić, Vanja; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2004)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1218
AB  - Pathological resorptions of a tooth root very often represent both a diagnostic and therapeutical problem. The goal of this study was, based on clinical research on a number of pathological resorptions of various types and localization in relation to time, to analyze and evaluate applied endodontic therapy. Clinical research covered 30 patients (40 root canals in total). 18 teeth (22 canal) with external and 12 (18 canal) with internal root resorptions received endodontic therapy. After standard endodontic procedure had been applied (cleaning and canal shaping) calcium hydroxide paste was used as a canal medicament for cases with internal resorptions and calcium hydroxide paste and points for cases with external resorptions. Canal opturations were done by modified technique of lateral condesation applying AH- Plus paste. The obtained results showed that in cases of internal resorption after 12 months, 11 cases were successful and only 1 failure occurred. In case of external resorption, after the same period of time, 15 cases were successful and failures occurred in 3 cases. The success of resorption treatment depends on many factors but, primarily, it depends on time and accuracy of diagnosis and properly applied treatment and root canal opturation.
AB  - Patološke resorpcije na korenu zuba vrlo često predstavljaju i dijagnostič ki i terapijski problem. Cilj ovog rada bio je da se na osnovu kliničkih istraživanja na izvesnom broju tretiranih slučajeva patoloških resorpcija različitog tipa i lokalizacije u funkciji vremena analizira evolucija i uspeh preduzete endodontske terapije. Klinička ispitivanja obuhvatila su 30 pacijenata (40 kanala). Endodontski je lečeno 18 zuba (22 kanala) sa eksternim resorpcijama i 12 zuba (18 kanala) sa resorpcijama internog porekla. Nakon standardno sprovedene endodontske procedure (čišćenje i oblikovanje kanala) kao kanalna medikacija je korišćena pasta kalcijum hidroksida kod slučajeva sa internim resorpcijama, odnosno pasta kalcijum hidroksida ili štapići kalcijum hidroksida kod zuba sa eksternim resorpcijama. Opturacija kanala kod svih zuba urađena je modifikovanom tehnikom lateralne kondenzacije uz primenu paste AH-Plus. Dobijeni rezultati su pokazali da je kod slučajeva sa internim resorpcijama kod 11 slučajeva konstatovan uspešan ishod, a samo u jednom slučaju pogoršanje i kliničke i rendgenološ ke slike u periodu do 12 meseci. Kod eksternih resorpcija uspeh endodontskog lečenja je zabeležen u 15 slučajeva, a neuspeh u 3 slučaja. Uspeh terapije radiksnih resorpcija zavisi od više faktora, ali pre svega od pravovremeno postavljene dijagnoze i pravilno sprovedene obrade i opturacije kanalskog sistema zuba.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Endodontic therapy of pathological resorptions of tooth root
T1  - Endodontska terapija patoloških resorpcija korena zuba
VL  - 51
IS  - 3
SP  - 130
EP  - 135
DO  - 10.2298/SGS0403130O
ER  - 
@article{
author = "Opačić-Galić, Vanja and Živković, Slavoljub",
year = "2004",
abstract = "Pathological resorptions of a tooth root very often represent both a diagnostic and therapeutical problem. The goal of this study was, based on clinical research on a number of pathological resorptions of various types and localization in relation to time, to analyze and evaluate applied endodontic therapy. Clinical research covered 30 patients (40 root canals in total). 18 teeth (22 canal) with external and 12 (18 canal) with internal root resorptions received endodontic therapy. After standard endodontic procedure had been applied (cleaning and canal shaping) calcium hydroxide paste was used as a canal medicament for cases with internal resorptions and calcium hydroxide paste and points for cases with external resorptions. Canal opturations were done by modified technique of lateral condesation applying AH- Plus paste. The obtained results showed that in cases of internal resorption after 12 months, 11 cases were successful and only 1 failure occurred. In case of external resorption, after the same period of time, 15 cases were successful and failures occurred in 3 cases. The success of resorption treatment depends on many factors but, primarily, it depends on time and accuracy of diagnosis and properly applied treatment and root canal opturation., Patološke resorpcije na korenu zuba vrlo često predstavljaju i dijagnostič ki i terapijski problem. Cilj ovog rada bio je da se na osnovu kliničkih istraživanja na izvesnom broju tretiranih slučajeva patoloških resorpcija različitog tipa i lokalizacije u funkciji vremena analizira evolucija i uspeh preduzete endodontske terapije. Klinička ispitivanja obuhvatila su 30 pacijenata (40 kanala). Endodontski je lečeno 18 zuba (22 kanala) sa eksternim resorpcijama i 12 zuba (18 kanala) sa resorpcijama internog porekla. Nakon standardno sprovedene endodontske procedure (čišćenje i oblikovanje kanala) kao kanalna medikacija je korišćena pasta kalcijum hidroksida kod slučajeva sa internim resorpcijama, odnosno pasta kalcijum hidroksida ili štapići kalcijum hidroksida kod zuba sa eksternim resorpcijama. Opturacija kanala kod svih zuba urađena je modifikovanom tehnikom lateralne kondenzacije uz primenu paste AH-Plus. Dobijeni rezultati su pokazali da je kod slučajeva sa internim resorpcijama kod 11 slučajeva konstatovan uspešan ishod, a samo u jednom slučaju pogoršanje i kliničke i rendgenološ ke slike u periodu do 12 meseci. Kod eksternih resorpcija uspeh endodontskog lečenja je zabeležen u 15 slučajeva, a neuspeh u 3 slučaja. Uspeh terapije radiksnih resorpcija zavisi od više faktora, ali pre svega od pravovremeno postavljene dijagnoze i pravilno sprovedene obrade i opturacije kanalskog sistema zuba.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Endodontic therapy of pathological resorptions of tooth root, Endodontska terapija patoloških resorpcija korena zuba",
volume = "51",
number = "3",
pages = "130-135",
doi = "10.2298/SGS0403130O"
}
Opačić-Galić, V.,& Živković, S.. (2004). Endodontic therapy of pathological resorptions of tooth root. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 51(3), 130-135.
https://doi.org/10.2298/SGS0403130O
Opačić-Galić V, Živković S. Endodontic therapy of pathological resorptions of tooth root. in Stomatološki glasnik Srbije. 2004;51(3):130-135.
doi:10.2298/SGS0403130O .
Opačić-Galić, Vanja, Živković, Slavoljub, "Endodontic therapy of pathological resorptions of tooth root" in Stomatološki glasnik Srbije, 51, no. 3 (2004):130-135,
https://doi.org/10.2298/SGS0403130O . .
1

Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers

Živković, Slavoljub; Stevanović, Ružica; Dačić, Dragoslav; Opačić-Galić, Vanja; Pavlović, Violeta

(Udruženje stomatologa Balkana, 2004)

TY  - JOUR
AU  - Živković, Slavoljub
AU  - Stevanović, Ružica
AU  - Dačić, Dragoslav
AU  - Opačić-Galić, Vanja
AU  - Pavlović, Violeta
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1213
AB  - Objective: The purpose of an in vitro dye leakage study was to determine the influence of smear layer on the apical leakage of the root canals obturated by lateral condensation using 3 different root canal sealers. Material and Method: The root canals of 120 extracted human teeth were instrumented by step-back technique with HEDSTRÖM file and irrigated with 2.5 NaOCl. The teeth were divided into 2 experimental groups. In the first group the root canal was treated with 10 ml 17% REDTA and 5.25% NaOCl to remove the smear layer. In the second group the root canal was not pre-treated for the removal of the smear layer. All teeth were obturated by lateral condensation technique using three different root canal sealers: AH-26, RSA-Roeko Seal Automix and Ketac-Endo. The teeth were covered with nail varnish excluding 1 mm on the apex and were immersed in 50% solution of silver nitrate for 6 hours. After this period, the teeth were washed and cut longitudinally for apical leakage analysis. The linear breakthrough of the dye was estimated using binocular magnifying class that magnifies 25 times. Results: The results showed less apical leakage in the first group where smear layer was removed: AH-26 (0.81), RSA (0.86) and Ketac-Endo (1.12) in comparison to the group where smear layer was not removed: AH-26 (0.94), RSA (0.98) and Ketac-Endo (1.32). Statistical evaluation of the results showed no significant difference in the leakage between the groups with and without the smear layer. However, RSA and AH-26 were statistically different from Ketac Endo (p lt 0.001) and among the sealers. Conclusion: Used endodontic sealers allowed leakage to occur, and removal of the smear layer reduced apical leakage.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers
VL  - 8
IS  - 2
SP  - 119
EP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_smile_1213
ER  - 
@article{
author = "Živković, Slavoljub and Stevanović, Ružica and Dačić, Dragoslav and Opačić-Galić, Vanja and Pavlović, Violeta",
year = "2004",
abstract = "Objective: The purpose of an in vitro dye leakage study was to determine the influence of smear layer on the apical leakage of the root canals obturated by lateral condensation using 3 different root canal sealers. Material and Method: The root canals of 120 extracted human teeth were instrumented by step-back technique with HEDSTRÖM file and irrigated with 2.5 NaOCl. The teeth were divided into 2 experimental groups. In the first group the root canal was treated with 10 ml 17% REDTA and 5.25% NaOCl to remove the smear layer. In the second group the root canal was not pre-treated for the removal of the smear layer. All teeth were obturated by lateral condensation technique using three different root canal sealers: AH-26, RSA-Roeko Seal Automix and Ketac-Endo. The teeth were covered with nail varnish excluding 1 mm on the apex and were immersed in 50% solution of silver nitrate for 6 hours. After this period, the teeth were washed and cut longitudinally for apical leakage analysis. The linear breakthrough of the dye was estimated using binocular magnifying class that magnifies 25 times. Results: The results showed less apical leakage in the first group where smear layer was removed: AH-26 (0.81), RSA (0.86) and Ketac-Endo (1.12) in comparison to the group where smear layer was not removed: AH-26 (0.94), RSA (0.98) and Ketac-Endo (1.32). Statistical evaluation of the results showed no significant difference in the leakage between the groups with and without the smear layer. However, RSA and AH-26 were statistically different from Ketac Endo (p lt 0.001) and among the sealers. Conclusion: Used endodontic sealers allowed leakage to occur, and removal of the smear layer reduced apical leakage.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers",
volume = "8",
number = "2",
pages = "119-122",
url = "https://hdl.handle.net/21.15107/rcub_smile_1213"
}
Živković, S., Stevanović, R., Dačić, D., Opačić-Galić, V.,& Pavlović, V.. (2004). Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 8(2), 119-122.
https://hdl.handle.net/21.15107/rcub_smile_1213
Živković S, Stevanović R, Dačić D, Opačić-Galić V, Pavlović V. Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers. in Balkan Journal of Stomatology. 2004;8(2):119-122.
https://hdl.handle.net/21.15107/rcub_smile_1213 .
Živković, Slavoljub, Stevanović, Ružica, Dačić, Dragoslav, Opačić-Galić, Vanja, Pavlović, Violeta, "Influence of smear layer on the apical leakage of 1 new and 2 old canal sealers" in Balkan Journal of Stomatology, 8, no. 2 (2004):119-122,
https://hdl.handle.net/21.15107/rcub_smile_1213 .

Frequency of the external resorptions of root apex

Opačić-Galić, Vanja; Živković, Slavoljub

(Srpsko lekarsko društvo, Beograd, 2004)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Živković, Slavoljub
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1205
AB  - Root resorptions present a significant problem in endodontic therapy of the affected teeth and in dentistry in general. The objective of this study was to analyze, based on epidemiological and statistical research, the frequency of clinical incidence of pathological root resorptions in everyday practice related to localization, type of tooth, age and sex of patients. Radiographie documentation of patients treated from 1997 till 2002 at the Department of Conservative Dentistry and Endodontics, Faculty of Stomatology in Belgrade, was used as baseline for this study. Retroalveolar radiographs of teeth with visible signs of resorptions were singled out from 15654 patients' clinical records used for this study. The external resorptions were shown as radiolucent areas localized on various outer root surfaces, followed by significant or less significant resorption of lamina dura and alveolar bone. Out of all teeth analyzed in this study, 594 (3.79%) showed some kind of resorption. The external resorptions were found to be more present in the upper jaw (55.10%) and molars (50.30%) than in the lower jaw (44.90%) and single root teeth (49.70%), but in both cases without significant statistical differences. The most frequent localization of resorptions was root apex (82.44%). In regard to age, the most frequent resorptions were recorded in patients aged between 21 and 30 years (28.40%), and the lowest incidence was found in the youngest population (5.51%). The results also showed that resorptions were more frequent among the female population (59.04%) than among the male population (40.96%). Based on these results, we may conclude that the external root resorptions are not a frequent clinical phenomenon. Proper and early diagnostics of such tissue pathology is one of the basic prerequisites for successful endodontic therapy of the affected root.
AB  - Resorpcije korena zuba predstavljaju značajan problem u endodontskoj terapiji obolelih zuba i stomatološkoj praksi uopšte. Cilj ovog rada je da se na osnovu epidemiološko-statističkih istraživanja proveri učestalost kliničkih pojava patoloških resorpcija na korenu zuba bolesnika iz svakodnevne prakse u odnosu na vrstu zuba, lokalizaciju resorpcije, starosno doba i pol osobe sa resorptivnim promenama. Kao materijal za ovu studiju korišćena je rendgenološka dokumentacija bolesnika Klinike za bolesti zuba Stomatološkog fakulteta u Beogradu koji su lečeni od 1997. do 2002. godine. Ispitivanjem su obuhvaćeni podaci iz 15.654 klinička kartona bolesnika, iz kojih su izdvajani retroalveolarni rendgenografski snimci zuba sa vidljivim resorpcijama. Eksterne resorpcije su konstatovane u vidu rendgenoloških rasvetljenja na različitim lokalizacijama spoljašnje površine korena zuba, praćenih manjom ili većom resorpcijom lamine dure ili alveolarne kosti. Dobijeni rezultati su pokazali da je od ukupnog broja pregledanih zuba u 594 slučaja otkriven neki oblik resorpcije korena (3,79%). Eksterne resorpcije bile su češće kod gornjih (55,1%) i kod višekorenih zuba (50,3%), nego kod zuba donje vilice (44,9%) i jednokorenih zuba (49,7%), ali bez statistički značajnih razlika. Najčešća lokalizacija resorpcija bio je apeks korena zuba (82,44%). Eksterne resorpcije korena najčešće se javljaju u periodu od 21. do 30. godine (28,4%), a najmanji procenat otkriven je u populaciji od 18 do 20 godina (5,51%). Kod osoba ženskog pola (59,04%) znatno je češća pojava eksternih resorpcija nego kod muškaraca (40,96%). Na osnovu dobijenih rezultata može ce zaključiti da su eksterne resorpcije korena redak klinički fenomen. Budući da ovakve lezije mogu uticati i na opšte zdravlje bolesnika, pravilna i pravovremena dijagnostika ove patologije zubnih tkiva je jedan od osnovnih preduslova za uspešnu endodontsku terapiju korena obolelog zuba, odnosno prevenciju opštih oboljenja.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Frequency of the external resorptions of root apex
T1  - Učestalost eksternih resorpcija na korenu zuba
VL  - 132
IS  - 5-6
SP  - 152
EP  - 156
DO  - 10.2298/SARH0406152O
ER  - 
@article{
author = "Opačić-Galić, Vanja and Živković, Slavoljub",
year = "2004",
abstract = "Root resorptions present a significant problem in endodontic therapy of the affected teeth and in dentistry in general. The objective of this study was to analyze, based on epidemiological and statistical research, the frequency of clinical incidence of pathological root resorptions in everyday practice related to localization, type of tooth, age and sex of patients. Radiographie documentation of patients treated from 1997 till 2002 at the Department of Conservative Dentistry and Endodontics, Faculty of Stomatology in Belgrade, was used as baseline for this study. Retroalveolar radiographs of teeth with visible signs of resorptions were singled out from 15654 patients' clinical records used for this study. The external resorptions were shown as radiolucent areas localized on various outer root surfaces, followed by significant or less significant resorption of lamina dura and alveolar bone. Out of all teeth analyzed in this study, 594 (3.79%) showed some kind of resorption. The external resorptions were found to be more present in the upper jaw (55.10%) and molars (50.30%) than in the lower jaw (44.90%) and single root teeth (49.70%), but in both cases without significant statistical differences. The most frequent localization of resorptions was root apex (82.44%). In regard to age, the most frequent resorptions were recorded in patients aged between 21 and 30 years (28.40%), and the lowest incidence was found in the youngest population (5.51%). The results also showed that resorptions were more frequent among the female population (59.04%) than among the male population (40.96%). Based on these results, we may conclude that the external root resorptions are not a frequent clinical phenomenon. Proper and early diagnostics of such tissue pathology is one of the basic prerequisites for successful endodontic therapy of the affected root., Resorpcije korena zuba predstavljaju značajan problem u endodontskoj terapiji obolelih zuba i stomatološkoj praksi uopšte. Cilj ovog rada je da se na osnovu epidemiološko-statističkih istraživanja proveri učestalost kliničkih pojava patoloških resorpcija na korenu zuba bolesnika iz svakodnevne prakse u odnosu na vrstu zuba, lokalizaciju resorpcije, starosno doba i pol osobe sa resorptivnim promenama. Kao materijal za ovu studiju korišćena je rendgenološka dokumentacija bolesnika Klinike za bolesti zuba Stomatološkog fakulteta u Beogradu koji su lečeni od 1997. do 2002. godine. Ispitivanjem su obuhvaćeni podaci iz 15.654 klinička kartona bolesnika, iz kojih su izdvajani retroalveolarni rendgenografski snimci zuba sa vidljivim resorpcijama. Eksterne resorpcije su konstatovane u vidu rendgenoloških rasvetljenja na različitim lokalizacijama spoljašnje površine korena zuba, praćenih manjom ili većom resorpcijom lamine dure ili alveolarne kosti. Dobijeni rezultati su pokazali da je od ukupnog broja pregledanih zuba u 594 slučaja otkriven neki oblik resorpcije korena (3,79%). Eksterne resorpcije bile su češće kod gornjih (55,1%) i kod višekorenih zuba (50,3%), nego kod zuba donje vilice (44,9%) i jednokorenih zuba (49,7%), ali bez statistički značajnih razlika. Najčešća lokalizacija resorpcija bio je apeks korena zuba (82,44%). Eksterne resorpcije korena najčešće se javljaju u periodu od 21. do 30. godine (28,4%), a najmanji procenat otkriven je u populaciji od 18 do 20 godina (5,51%). Kod osoba ženskog pola (59,04%) znatno je češća pojava eksternih resorpcija nego kod muškaraca (40,96%). Na osnovu dobijenih rezultata može ce zaključiti da su eksterne resorpcije korena redak klinički fenomen. Budući da ovakve lezije mogu uticati i na opšte zdravlje bolesnika, pravilna i pravovremena dijagnostika ove patologije zubnih tkiva je jedan od osnovnih preduslova za uspešnu endodontsku terapiju korena obolelog zuba, odnosno prevenciju opštih oboljenja.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Frequency of the external resorptions of root apex, Učestalost eksternih resorpcija na korenu zuba",
volume = "132",
number = "5-6",
pages = "152-156",
doi = "10.2298/SARH0406152O"
}
Opačić-Galić, V.,& Živković, S.. (2004). Frequency of the external resorptions of root apex. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 132(5-6), 152-156.
https://doi.org/10.2298/SARH0406152O
Opačić-Galić V, Živković S. Frequency of the external resorptions of root apex. in Srpski arhiv za celokupno lekarstvo. 2004;132(5-6):152-156.
doi:10.2298/SARH0406152O .
Opačić-Galić, Vanja, Živković, Slavoljub, "Frequency of the external resorptions of root apex" in Srpski arhiv za celokupno lekarstvo, 132, no. 5-6 (2004):152-156,
https://doi.org/10.2298/SARH0406152O . .
6
5

Epidemiološko-klinička ispitivanja radiksnih resorpcija

Opačić-Galić, Vanja

(Univerzitet u Beogradu, Stomatološki fakultet, 2003)

TY  - THES
AU  - Opačić-Galić, Vanja
PY  - 2003
UR  - https://plus.sr.cobiss.net/opac7/bib/1024032910
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/388
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Epidemiološko-klinička ispitivanja radiksnih resorpcija
UR  - https://hdl.handle.net/21.15107/rcub_smile_388
ER  - 
@mastersthesis{
author = "Opačić-Galić, Vanja",
year = "2003",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Epidemiološko-klinička ispitivanja radiksnih resorpcija",
url = "https://hdl.handle.net/21.15107/rcub_smile_388"
}
Opačić-Galić, V.. (2003). Epidemiološko-klinička ispitivanja radiksnih resorpcija. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_smile_388
Opačić-Galić V. Epidemiološko-klinička ispitivanja radiksnih resorpcija. 2003;.
https://hdl.handle.net/21.15107/rcub_smile_388 .
Opačić-Galić, Vanja, "Epidemiološko-klinička ispitivanja radiksnih resorpcija" (2003),
https://hdl.handle.net/21.15107/rcub_smile_388 .