TY  - JOUR
AU  - Čolović, Božana
AU  - Janković, Ognjenka
AU  - Živković, Slavoljub
AU  - Zizak, Zeljko
AU  - Zizak, Irina Besu
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2442
AB  - The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.
PB  - Elsevier Sci Ltd, Oxford
T2  - Ceramics International
T1  - A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
VL  - 45
IS  - 7
SP  - 9211
EP  - 9218
DO  - 10.1016/j.ceramint.2019.01.266
ER  - 

@article{
author = "Čolović, Božana and Janković, Ognjenka and Živković, Slavoljub and Zizak, Zeljko and Zizak, Irina Besu and Jokanović, Vukoman",
year = "2019",
abstract = "The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Ceramics International",
title = "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis",
volume = "45",
number = "7",
pages = "9211-9218",
doi = "10.1016/j.ceramint.2019.01.266"
}

Čolović, B., Janković, O., Živković, S., Zizak, Z., Zizak, I. B.,& Jokanović, V.. (2019). A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International
Elsevier Sci Ltd, Oxford., 45(7), 9211-9218.
https://doi.org/10.1016/j.ceramint.2019.01.266

Čolović B, Janković O, Živković S, Zizak Z, Zizak IB, Jokanović V. A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International. 2019;45(7):9211-9218.
doi:10.1016/j.ceramint.2019.01.266 .

Čolović, Božana, Janković, Ognjenka, Živković, Slavoljub, Zizak, Zeljko, Zizak, Irina Besu, Jokanović, Vukoman, "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis" in Ceramics International, 45, no. 7 (2019):9211-9218,
https://doi.org/10.1016/j.ceramint.2019.01.266 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Ćetenović, Bojana
AU  - Živković, Slavoljub
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2427
AB  - Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties.
AB  - U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics
T1  - Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji
VL  - 66
IS  - 2
SP  - 87
EP  - 96
DO  - 10.2478/sdj-2019-0010
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Ćetenović, Bojana and Živković, Slavoljub",
year = "2019",
abstract = "Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties., U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics, Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji",
volume = "66",
number = "2",
pages = "87-96",
doi = "10.2478/sdj-2019-0010"
}

Jokanović, V., Čolović, B., Ćetenović, B.,& Živković, S.. (2019). Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 66(2), 87-96.
https://doi.org/10.2478/sdj-2019-0010

Jokanović V, Čolović B, Ćetenović B, Živković S. Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije. 2019;66(2):87-96.
doi:10.2478/sdj-2019-0010 .

Jokanović, Vukoman, Čolović, Božana, Ćetenović, Bojana, Živković, Slavoljub, "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics" in Stomatološki glasnik Srbije, 66, no. 2 (2019):87-96,
https://doi.org/10.2478/sdj-2019-0010 . .

TY  - JOUR
AU  - Paras, S.
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Mitrović-Ajtić, O.
AU  - Dekić, R.
AU  - Soldatović, Ivan
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2393
AB  - Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
VL  - 52
IS  - 8
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 

@article{
author = "Paras, S. and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana and Mitrović-Ajtić, O. and Dekić, R. and Soldatović, Ivan and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman",
year = "2019",
abstract = "Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
volume = "52",
number = "8",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}

Paras, S., Janković, O., Trišić, D., Čolović, B., Mitrović-Ajtić, O., Dekić, R., Soldatović, I., Živković-Sandić, M., Živković, S.,& Jokanović, V.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal
Wiley, Hoboken., 52(8), 1162-1172.
https://doi.org/10.1111/iej.13105

Paras S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković-Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;52(8):1162-1172.
doi:10.1111/iej.13105 .

Paras, S., Janković, Ognjenka, Trišić, Dijana, Čolović, Božana, Mitrović-Ajtić, O., Dekić, R., Soldatović, Ivan, Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman, "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal, 52, no. 8 (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .

TY  - JOUR
AU  - Magić, Marko
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Vasilijić, Saša
AU  - Marković, Milan
AU  - Vučević, Dragana
AU  - Rudolf, Rebeka
AU  - Čolić, Snježana
AU  - Čolić, Miodrag
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2445
AB  - Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo.
AB  - Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition
T1  - Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme
VL  - 76
IS  - 5
SP  - 492
EP  - 501
DO  - 10.2298/VSP170809097M
ER  - 

@article{
author = "Magić, Marko and Čolović, Božana and Jokanović, Vukoman and Vasilijić, Saša and Marković, Milan and Vučević, Dragana and Rudolf, Rebeka and Čolić, Snježana and Čolić, Miodrag",
year = "2019",
abstract = "Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo., Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition, Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme",
volume = "76",
number = "5",
pages = "492-501",
doi = "10.2298/VSP170809097M"
}

Magić, M., Čolović, B., Jokanović, V., Vasilijić, S., Marković, M., Vučević, D., Rudolf, R., Čolić, S.,& Čolić, M.. (2019). Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(5), 492-501.
https://doi.org/10.2298/VSP170809097M

Magić M, Čolović B, Jokanović V, Vasilijić S, Marković M, Vučević D, Rudolf R, Čolić S, Čolić M. Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled. 2019;76(5):492-501.
doi:10.2298/VSP170809097M .

Magić, Marko, Čolović, Božana, Jokanović, Vukoman, Vasilijić, Saša, Marković, Milan, Vučević, Dragana, Rudolf, Rebeka, Čolić, Snježana, Čolić, Miodrag, "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition" in Vojnosanitetski pregled, 76, no. 5 (2019):492-501,
https://doi.org/10.2298/VSP170809097M . .

TY  - JOUR
AU  - Ilić, Dragan
AU  - Antonijević, Đorđe
AU  - Biočanin, Vladimir
AU  - Čolović, Božana
AU  - Danilović, Vesna
AU  - Komlev, Vladimir S.
AU  - Teterina, Anastasia
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2411
AB  - Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Physico-chemical and biological properties of dental calcium silicate cements - literature review
VL  - 73
IS  - 5
SP  - 281
EP  - 294
DO  - 10.2298/HEMIND190614027I
ER  - 

@article{
author = "Ilić, Dragan and Antonijević, Đorđe and Biočanin, Vladimir and Čolović, Božana and Danilović, Vesna and Komlev, Vladimir S. and Teterina, Anastasia and Jokanović, Vukoman",
year = "2019",
abstract = "Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Physico-chemical and biological properties of dental calcium silicate cements - literature review",
volume = "73",
number = "5",
pages = "281-294",
doi = "10.2298/HEMIND190614027I"
}

Ilić, D., Antonijević, Đ., Biočanin, V., Čolović, B., Danilović, V., Komlev, V. S., Teterina, A.,& Jokanović, V.. (2019). Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 73(5), 281-294.
https://doi.org/10.2298/HEMIND190614027I

Ilić D, Antonijević Đ, Biočanin V, Čolović B, Danilović V, Komlev VS, Teterina A, Jokanović V. Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija. 2019;73(5):281-294.
doi:10.2298/HEMIND190614027I .

Ilić, Dragan, Antonijević, Đorđe, Biočanin, Vladimir, Čolović, Božana, Danilović, Vesna, Komlev, Vladimir S., Teterina, Anastasia, Jokanović, Vukoman, "Physico-chemical and biological properties of dental calcium silicate cements - literature review" in Hemijska Industrija, 73, no. 5 (2019):281-294,
https://doi.org/10.2298/HEMIND190614027I . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Prokić, Bogomir Bolka
AU  - Tomanović, Nada
AU  - Popović-Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2313
AB  - As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.
PB  - Hindawi Ltd, London
T2  - Advances in Materials Science & Engineering
T1  - Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates
VL  - 2018
DO  - 10.1155/2018/8493439
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Prokić, Bogomir Bolka and Tomanović, Nada and Popović-Bajić, Marijana and Živković, Slavoljub",
year = "2018",
abstract = "As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.",
publisher = "Hindawi Ltd, London",
journal = "Advances in Materials Science & Engineering",
title = "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates",
volume = "2018",
doi = "10.1155/2018/8493439"
}

Jokanović, V., Čolović, B., Prokić, B. B., Tomanović, N., Popović-Bajić, M.,& Živković, S.. (2018). Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering
Hindawi Ltd, London., 2018.
https://doi.org/10.1155/2018/8493439

Jokanović V, Čolović B, Prokić BB, Tomanović N, Popović-Bajić M, Živković S. Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering. 2018;2018.
doi:10.1155/2018/8493439 .

Jokanović, Vukoman, Čolović, Božana, Prokić, Bogomir Bolka, Tomanović, Nada, Popović-Bajić, Marijana, Živković, Slavoljub, "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates" in Advances in Materials Science & Engineering, 2018 (2018),
https://doi.org/10.1155/2018/8493439 . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana
AU  - Vasilijić, Saša
AU  - Prokić, Bogomir
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman
AU  - Tepavčević, Zvezdana
AU  - Marković, Dejan
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2275
AB  - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study
VL  - 29
IS  - 12
DO  - 10.1007/s10856-018-6200-z
ER  - 

@article{
author = "Ćetenović, Bojana and Čolović, Božana and Vasilijić, Saša and Prokić, Bogomir and Pašalić, Snežana and Jokanović, Vukoman and Tepavčević, Zvezdana and Marković, Dejan",
year = "2018",
abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study",
volume = "29",
number = "12",
doi = "10.1007/s10856-018-6200-z"
}

Ćetenović, B., Čolović, B., Vasilijić, S., Prokić, B., Pašalić, S., Jokanović, V., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 29(12).
https://doi.org/10.1007/s10856-018-6200-z

Ćetenović B, Čolović B, Vasilijić S, Prokić B, Pašalić S, Jokanović V, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine. 2018;29(12).
doi:10.1007/s10856-018-6200-z .

Ćetenović, Bojana, Čolović, Božana, Vasilijić, Saša, Prokić, Bogomir, Pašalić, Snežana, Jokanović, Vukoman, Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study" in Journal of Materials Science-Materials in Medicine, 29, no. 12 (2018),
https://doi.org/10.1007/s10856-018-6200-z . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Soldatović, Ivan
AU  - Antonijević, Đorđe
AU  - Milosavljević, Petar
AU  - Sjerobabin, Nikola
AU  - Sopta, Jelena
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2187
AB  - This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedical Engineering - Biomedizinische Technik
T1  - Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation
VL  - 62
IS  - 3
SP  - 295
EP  - 306
DO  - 10.1515/bmt-2015-0164
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Soldatović, Ivan and Antonijević, Đorđe and Milosavljević, Petar and Sjerobabin, Nikola and Sopta, Jelena",
year = "2017",
abstract = "This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedical Engineering - Biomedizinische Technik",
title = "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation",
volume = "62",
number = "3",
pages = "295-306",
doi = "10.1515/bmt-2015-0164"
}

Jokanović, V., Čolović, B., Marković, D., Petrović, M., Soldatović, I., Antonijević, Đ., Milosavljević, P., Sjerobabin, N.,& Sopta, J.. (2017). Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin., 62(3), 295-306.
https://doi.org/10.1515/bmt-2015-0164

Jokanović V, Čolović B, Marković D, Petrović M, Soldatović I, Antonijević Đ, Milosavljević P, Sjerobabin N, Sopta J. Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik. 2017;62(3):295-306.
doi:10.1515/bmt-2015-0164 .

Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Soldatović, Ivan, Antonijević, Đorđe, Milosavljević, Petar, Sjerobabin, Nikola, Sopta, Jelena, "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation" in Biomedical Engineering - Biomedizinische Technik, 62, no. 3 (2017):295-306,
https://doi.org/10.1515/bmt-2015-0164 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Antonijević, Đorđe
AU  - Mićić, Milutin
AU  - Živković, Slavoljub
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2242
AB  - There is growing need for synthetic tissue replacement materials designed in a way that mimic complex structure of tissues and organs. Among various methods for fabrication of implants (scaffolds), 3D printing is very powerful technique because it enables creation of scaffolds with complex internal structures and high resolution, based on medical data sets. This method allows fabrication of scaffolds with desired macro- and micro-porosity and fully inter- connected pore network. Rapid development of 3D printing technologies has enabled various applications from the creation of anatomical training models for complex surgical procedures to the printing of tissue engineering constructs. The aim of current investigations was to develop compatible printers and materials (bioinks) to obtain biomimetic scaffolds, which allow printing of living cells without significant loss of cell viability. The advanced level of such printing assumes “in situ” printing, i.e. printing cells and biomaterials directly onto or in a patient that will reduce recovery time.
AB  - Danas postoji sve veća potreba za sintetičkim materijalima za zamenu tkiva dizajniranih na način koji imitira složenu strukturu tkiva i organa. Među različitim metodama proizvodnje implantata (skafolda), 3D štampanje je veoma moćna tehnika jer omogućava kreiranje skafolda sa složenim unutrašnjim strukturama i visokom rezolucijom, zasnovanim na medicinskim skupovima podataka. Ova metoda omogućava proizvodnju skafolda sa željenom makroporoznošću i mikroporoznošću i potpuno povezanom mrežom pora. Brzi razvoj tehnologija 3D štampanja omogućio je različite primene – od kreiranja anatomskih modela za uvežbavanje složenih hirurških procedura do štampanja konstrukata za tkivno inženjerstvo. Cilj tekućih istraživanja je razvoj kompatibilnih štampača i materijala (bio-mastila) za dobijanje biomimičnih skafolda, koji omogućavaju štampanje živih ćelija bez značajnog gubitka njihove vijabilnosti. Napredni nivo takvog štampanja pretpostavlja štampanje in situ, tj. štampanje ćelija i biomaterijala direktno na pacijentu ili u pacijenta, što će smanjiti vreme oporavka.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Various methods of 3D and bio-printing
T1  - Različite metode 3D štampanja i bio-štampanja
VL  - 64
IS  - 3
SP  - 136
EP  - 145
DO  - 10.1515/sdj-2017-0014
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Antonijević, Đorđe and Mićić, Milutin and Živković, Slavoljub",
year = "2017",
abstract = "There is growing need for synthetic tissue replacement materials designed in a way that mimic complex structure of tissues and organs. Among various methods for fabrication of implants (scaffolds), 3D printing is very powerful technique because it enables creation of scaffolds with complex internal structures and high resolution, based on medical data sets. This method allows fabrication of scaffolds with desired macro- and micro-porosity and fully inter- connected pore network. Rapid development of 3D printing technologies has enabled various applications from the creation of anatomical training models for complex surgical procedures to the printing of tissue engineering constructs. The aim of current investigations was to develop compatible printers and materials (bioinks) to obtain biomimetic scaffolds, which allow printing of living cells without significant loss of cell viability. The advanced level of such printing assumes “in situ” printing, i.e. printing cells and biomaterials directly onto or in a patient that will reduce recovery time., Danas postoji sve veća potreba za sintetičkim materijalima za zamenu tkiva dizajniranih na način koji imitira složenu strukturu tkiva i organa. Među različitim metodama proizvodnje implantata (skafolda), 3D štampanje je veoma moćna tehnika jer omogućava kreiranje skafolda sa složenim unutrašnjim strukturama i visokom rezolucijom, zasnovanim na medicinskim skupovima podataka. Ova metoda omogućava proizvodnju skafolda sa željenom makroporoznošću i mikroporoznošću i potpuno povezanom mrežom pora. Brzi razvoj tehnologija 3D štampanja omogućio je različite primene – od kreiranja anatomskih modela za uvežbavanje složenih hirurških procedura do štampanja konstrukata za tkivno inženjerstvo. Cilj tekućih istraživanja je razvoj kompatibilnih štampača i materijala (bio-mastila) za dobijanje biomimičnih skafolda, koji omogućavaju štampanje živih ćelija bez značajnog gubitka njihove vijabilnosti. Napredni nivo takvog štampanja pretpostavlja štampanje in situ, tj. štampanje ćelija i biomaterijala direktno na pacijentu ili u pacijenta, što će smanjiti vreme oporavka.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Various methods of 3D and bio-printing, Različite metode 3D štampanja i bio-štampanja",
volume = "64",
number = "3",
pages = "136-145",
doi = "10.1515/sdj-2017-0014"
}

Jokanović, V., Čolović, B., Antonijević, Đ., Mićić, M.,& Živković, S.. (2017). Various methods of 3D and bio-printing. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 64(3), 136-145.
https://doi.org/10.1515/sdj-2017-0014

Jokanović V, Čolović B, Antonijević Đ, Mićić M, Živković S. Various methods of 3D and bio-printing. in Stomatološki glasnik Srbije. 2017;64(3):136-145.
doi:10.1515/sdj-2017-0014 .

Jokanović, Vukoman, Čolović, Božana, Antonijević, Đorđe, Mićić, Milutin, Živković, Slavoljub, "Various methods of 3D and bio-printing" in Stomatološki glasnik Srbije, 64, no. 3 (2017):136-145,
https://doi.org/10.1515/sdj-2017-0014 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Popović-Bajić, Marijana
AU  - Živković-Sandić, Marija
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2252
AB  - Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods.
AB  - Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Scaffold in bone tissue engineering
T1  - Skafoldi u inženjerstvu koštanog tkiva
VL  - 64
IS  - 1
SP  - 32
EP  - 40
DO  - 10.1515/sdj-2017-0004
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Popović-Bajić, Marijana and Živković-Sandić, Marija",
year = "2017",
abstract = "Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods., Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Scaffold in bone tissue engineering, Skafoldi u inženjerstvu koštanog tkiva",
volume = "64",
number = "1",
pages = "32-40",
doi = "10.1515/sdj-2017-0004"
}

Jokanović, V., Čolović, B., Popović-Bajić, M.,& Živković-Sandić, M.. (2017). Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 64(1), 32-40.
https://doi.org/10.1515/sdj-2017-0004

Jokanović V, Čolović B, Popović-Bajić M, Živković-Sandić M. Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije. 2017;64(1):32-40.
doi:10.1515/sdj-2017-0004 .

Jokanović, Vukoman, Čolović, Božana, Popović-Bajić, Marijana, Živković-Sandić, Marija, "Scaffold in bone tissue engineering" in Stomatološki glasnik Srbije, 64, no. 1 (2017):32-40,
https://doi.org/10.1515/sdj-2017-0004 . .

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana
AU  - Vasilijić, Saša
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2249
AB  - Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes after the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for further clinical studies.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - In vitro biocompatibility of nanostructured endodontic materials using SCAP cells
VL  - 21
IS  - 3
SP  - 167
EP  - 170
DO  - 10.1515/bjdm-2017-0029
ER  - 

@article{
author = "Ćetenović, Bojana and Čolović, Božana and Vasilijić, Saša and Pašalić, Snežana and Jokanović, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes after the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for further clinical studies.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "In vitro biocompatibility of nanostructured endodontic materials using SCAP cells",
volume = "21",
number = "3",
pages = "167-170",
doi = "10.1515/bjdm-2017-0029"
}

Ćetenović, B., Čolović, B., Vasilijić, S., Pašalić, S., Jokanović, V.,& Marković, D.. (2017). In vitro biocompatibility of nanostructured endodontic materials using SCAP cells. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 21(3), 167-170.
https://doi.org/10.1515/bjdm-2017-0029

Ćetenović B, Čolović B, Vasilijić S, Pašalić S, Jokanović V, Marković D. In vitro biocompatibility of nanostructured endodontic materials using SCAP cells. in Balkan Journal of Dental Medicine. 2017;21(3):167-170.
doi:10.1515/bjdm-2017-0029 .

Ćetenović, Bojana, Čolović, Božana, Vasilijić, Saša, Pašalić, Snežana, Jokanović, Vukoman, Marković, Dejan, "In vitro biocompatibility of nanostructured endodontic materials using SCAP cells" in Balkan Journal of Dental Medicine, 21, no. 3 (2017):167-170,
https://doi.org/10.1515/bjdm-2017-0029 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Jokanović, Milan
AU  - Milosavljević, Petar
AU  - Sopta, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2168
AB  - A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.
PB  - Hindawi Ltd, London
T2  - Journal of Nanomaterials
T1  - In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA
VL  - 2016
DO  - 10.1155/2016/3948768
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Jokanović, Milan and Milosavljević, Petar and Sopta, Jelena",
year = "2016",
abstract = "A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Nanomaterials",
title = "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA",
volume = "2016",
doi = "10.1155/2016/3948768"
}

Jokanović, V., Čolović, B., Marković, D., Petrović, M., Jokanović, M., Milosavljević, P.,& Sopta, J.. (2016). In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/3948768

Jokanović V, Čolović B, Marković D, Petrović M, Jokanović M, Milosavljević P, Sopta J. In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials. 2016;2016.
doi:10.1155/2016/3948768 .

Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Jokanović, Milan, Milosavljević, Petar, Sopta, Jelena, "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA" in Journal of Nanomaterials, 2016 (2016),
https://doi.org/10.1155/2016/3948768 . .

TY  - JOUR
AU  - Sjerobabin, Nikola
AU  - Čolović, Božana
AU  - Petrović, Milan
AU  - Marković, Dejan
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2162
AB  - Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained.
AB  - Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design
T1  - Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom
VL  - 144
IS  - 5-6
SP  - 280
EP  - 287
DO  - 10.2298/SARH1606280S
ER  - 

@article{
author = "Sjerobabin, Nikola and Čolović, Božana and Petrović, Milan and Marković, Dejan and Živković, Slavoljub and Jokanović, Vukoman",
year = "2016",
abstract = "Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained., Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design, Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom",
volume = "144",
number = "5-6",
pages = "280-287",
doi = "10.2298/SARH1606280S"
}

Sjerobabin, N., Čolović, B., Petrović, M., Marković, D., Živković, S.,& Jokanović, V.. (2016). Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(5-6), 280-287.
https://doi.org/10.2298/SARH1606280S

Sjerobabin N, Čolović B, Petrović M, Marković D, Živković S, Jokanović V. Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):280-287.
doi:10.2298/SARH1606280S .

Sjerobabin, Nikola, Čolović, Božana, Petrović, Milan, Marković, Dejan, Živković, Slavoljub, Jokanović, Vukoman, "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):280-287,
https://doi.org/10.2298/SARH1606280S . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Živković-Sandić, Marija
AU  - Popović-Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2108
AB  - This paper analyzes mechanisms of fluorapatite mechanochemical synthesis and its structural characteristics. Several studies of Jokanovic et al. published in appropriate journals and the book 'Nanomedicine, the biggest challenge of the 21st century' are the base for this article. Characteristics of obtained materials show numerous biological advantages associated with the specific structural design of material during the process of synthesis. X-ray diffraction (XRD) and infrared spectroscopy with Fourier transform (FTIR) were used for studying the processes of fluorapatite synthesis.
AB  - U radu su analizirani mehanizmi mehanohemijske sinteze fluorapatita i njegove strukturne karakteristike. Suštinu istraživanja čine istraživanja V. Jokanovića i saradnika objavljena u odgovarajućim časopisima i knjizi Nanomedicina, najveći izazov 21. veka. Karakteristike dobijenih materijala pokazuju njihove brojne biološke prednosti, koje su povezane sa specifičnostima strukturnog dizajna materijala tokom procesa sinteze. Kao osnove za proučavanje procesa sinteze i mehanizma nastajanja fluorapatita korišćene su metode rendgenske difrakcije (XRD) i infracrvene spektroskopije sa Furijeovom transformacijom (FTIR).
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis
T1  - Strukturne karakteristike i mehanizmi mehanohemijske sinteze fluorapatita
VL  - 63
IS  - 2
SP  - 74
EP  - 84
DO  - 10.1515/sdj-2016-0008
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Živković-Sandić, Marija and Popović-Bajić, Marijana and Živković, Slavoljub",
year = "2016",
abstract = "This paper analyzes mechanisms of fluorapatite mechanochemical synthesis and its structural characteristics. Several studies of Jokanovic et al. published in appropriate journals and the book 'Nanomedicine, the biggest challenge of the 21st century' are the base for this article. Characteristics of obtained materials show numerous biological advantages associated with the specific structural design of material during the process of synthesis. X-ray diffraction (XRD) and infrared spectroscopy with Fourier transform (FTIR) were used for studying the processes of fluorapatite synthesis., U radu su analizirani mehanizmi mehanohemijske sinteze fluorapatita i njegove strukturne karakteristike. Suštinu istraživanja čine istraživanja V. Jokanovića i saradnika objavljena u odgovarajućim časopisima i knjizi Nanomedicina, najveći izazov 21. veka. Karakteristike dobijenih materijala pokazuju njihove brojne biološke prednosti, koje su povezane sa specifičnostima strukturnog dizajna materijala tokom procesa sinteze. Kao osnove za proučavanje procesa sinteze i mehanizma nastajanja fluorapatita korišćene su metode rendgenske difrakcije (XRD) i infracrvene spektroskopije sa Furijeovom transformacijom (FTIR).",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis, Strukturne karakteristike i mehanizmi mehanohemijske sinteze fluorapatita",
volume = "63",
number = "2",
pages = "74-84",
doi = "10.1515/sdj-2016-0008"
}

Jokanović, V., Čolović, B., Živković-Sandić, M., Popović-Bajić, M.,& Živković, S.. (2016). Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 63(2), 74-84.
https://doi.org/10.1515/sdj-2016-0008

Jokanović V, Čolović B, Živković-Sandić M, Popović-Bajić M, Živković S. Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis. in Stomatološki glasnik Srbije. 2016;63(2):74-84.
doi:10.1515/sdj-2016-0008 .

Jokanović, Vukoman, Čolović, Božana, Živković-Sandić, Marija, Popović-Bajić, Marijana, Živković, Slavoljub, "Structural characteristics and mechanisms of fluorapatite mechanochemical synthesis" in Stomatološki glasnik Srbije, 63, no. 2 (2016):74-84,
https://doi.org/10.1515/sdj-2016-0008 . .

TY  - CHAP
AU  - Marković, Dejan
AU  - Petrović, Bojan
AU  - Jokanović, Vukoman
AU  - Perić, Tamara
AU  - Čolović, Božana
AU  - Karadžić, Ivana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2163
AB  - Traditional strategies for skeletal regeneration in the orofacial region involve the use of autogenous and allogenic bone grafts that may not always be available or safe. One alternative is to develop materials for use as scaffolds for tissue engineering. These calcium-phosphate-based materials are porous, have a large surface area to volume ratio, and can be used to deliver drugs or cells. This enables the development of scaffolds for applications in tissue engineering and bone regeneration. Nanostructured materials promote greater amounts of specific protein interactions and more efficiently stimulate new bone formation. When features of scaffolds are nanoscaled, a variety of interactions can be stimulated at the cellular level. The main requirements for bone tissue engineering scaffolds are discussed, as well as the main types and design strategies. The mechanism by which nanomaterials promote bone formation is explained and the current research status of main types of nanostructured scaffolds is reviewed.
PB  - Elsevier Inc.
T2  - Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials
T1  - Nanomaterials as scaffolds in bone tissue engineering in dental medicine
SP  - 413
EP  - 442
DO  - 10.1016/B978-0-323-42862-0.00014-6
ER  - 

@inbook{
author = "Marković, Dejan and Petrović, Bojan and Jokanović, Vukoman and Perić, Tamara and Čolović, Božana and Karadžić, Ivana",
year = "2016",
abstract = "Traditional strategies for skeletal regeneration in the orofacial region involve the use of autogenous and allogenic bone grafts that may not always be available or safe. One alternative is to develop materials for use as scaffolds for tissue engineering. These calcium-phosphate-based materials are porous, have a large surface area to volume ratio, and can be used to deliver drugs or cells. This enables the development of scaffolds for applications in tissue engineering and bone regeneration. Nanostructured materials promote greater amounts of specific protein interactions and more efficiently stimulate new bone formation. When features of scaffolds are nanoscaled, a variety of interactions can be stimulated at the cellular level. The main requirements for bone tissue engineering scaffolds are discussed, as well as the main types and design strategies. The mechanism by which nanomaterials promote bone formation is explained and the current research status of main types of nanostructured scaffolds is reviewed.",
publisher = "Elsevier Inc.",
journal = "Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials",
booktitle = "Nanomaterials as scaffolds in bone tissue engineering in dental medicine",
pages = "413-442",
doi = "10.1016/B978-0-323-42862-0.00014-6"
}

Marković, D., Petrović, B., Jokanović, V., Perić, T., Čolović, B.,& Karadžić, I.. (2016). Nanomaterials as scaffolds in bone tissue engineering in dental medicine. in Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials
Elsevier Inc.., 413-442.
https://doi.org/10.1016/B978-0-323-42862-0.00014-6

Marković D, Petrović B, Jokanović V, Perić T, Čolović B, Karadžić I. Nanomaterials as scaffolds in bone tissue engineering in dental medicine. in Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials. 2016;:413-442.
doi:10.1016/B978-0-323-42862-0.00014-6 .

Marković, Dejan, Petrović, Bojan, Jokanović, Vukoman, Perić, Tamara, Čolović, Božana, Karadžić, Ivana, "Nanomaterials as scaffolds in bone tissue engineering in dental medicine" in Nanobiomaterials in Hard Tissue Engineering: Applications of Nanobiomaterials (2016):413-442,
https://doi.org/10.1016/B978-0-323-42862-0.00014-6 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Živković, Slavoljub
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2033
AB  - Bioactive glasses are often used nanomaterials in tissue engineering of bone and soft tissue. Many newly synthesized materials for applications in medicine and dentistry are based on these bioactive substances. Bioactive glass is usually used as a scaffold or as an implant coating on implants and it allows fast formation of apatite layer with positive effect on osteoblasts proliferation. These biomaterials play an important role in dentistry and endodontics. This study is mostly part of the monograph titled 'Nanomedicine, the Greatest Challenge of the 21st Century', that for two years has received attention from professional and scientific community in various fields. Information presented in this paper highlight structural characteristics of bioactive glasses that have a significant role in bone tissue engineering.
AB  - Bioaktivna stakla su nanomaterijali koji se dosta često koriste u inženjerstvu koštanih i mekih tkiva. Brojni novosintetisani materijali za primenu u medicini i stomatologiji se baziraju upravo na ovim bioaktivnim supstancama. Bioaktivno staklo se najčešće koristi kao skafold ili prevlaka na implantatima, i omogućava brzo formiranje apatitnog sloja, odnosno povoljno utiče na proliferaciju osteoblasta. Značaj ovih biomaterijala za primenu u stomatologiji i endodonciji zaslužuje posebno mesto, pa će im zato u okviru ovog rada biti posvećena posebna pažnja. I ovaj rad je najvećim delom sastavni deo monografije pod nazivom 'Nanomedicina, najveći izazov 21. veka', koja je već dve godine u žiži interesovanja stručne i naučne javnosti iz različitih oblasti. Informacije predstavljene u radu su vrlo značajne za teorijsko osvetljavanje značaja i strukturnih odlika bioaktivnih stakala, koji su od neprocenjivog značaja za inženjerstvo koštanih tkiva.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Bioactive glasses in bone tissue engineering
T1  - Bioaktivna stakla u inženjerstvu koštanih tkiva
VL  - 62
IS  - 2
SP  - 71
EP  - 79
DO  - 10.1515/sdj-2015-0008
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Živković, Slavoljub",
year = "2015",
abstract = "Bioactive glasses are often used nanomaterials in tissue engineering of bone and soft tissue. Many newly synthesized materials for applications in medicine and dentistry are based on these bioactive substances. Bioactive glass is usually used as a scaffold or as an implant coating on implants and it allows fast formation of apatite layer with positive effect on osteoblasts proliferation. These biomaterials play an important role in dentistry and endodontics. This study is mostly part of the monograph titled 'Nanomedicine, the Greatest Challenge of the 21st Century', that for two years has received attention from professional and scientific community in various fields. Information presented in this paper highlight structural characteristics of bioactive glasses that have a significant role in bone tissue engineering., Bioaktivna stakla su nanomaterijali koji se dosta često koriste u inženjerstvu koštanih i mekih tkiva. Brojni novosintetisani materijali za primenu u medicini i stomatologiji se baziraju upravo na ovim bioaktivnim supstancama. Bioaktivno staklo se najčešće koristi kao skafold ili prevlaka na implantatima, i omogućava brzo formiranje apatitnog sloja, odnosno povoljno utiče na proliferaciju osteoblasta. Značaj ovih biomaterijala za primenu u stomatologiji i endodonciji zaslužuje posebno mesto, pa će im zato u okviru ovog rada biti posvećena posebna pažnja. I ovaj rad je najvećim delom sastavni deo monografije pod nazivom 'Nanomedicina, najveći izazov 21. veka', koja je već dve godine u žiži interesovanja stručne i naučne javnosti iz različitih oblasti. Informacije predstavljene u radu su vrlo značajne za teorijsko osvetljavanje značaja i strukturnih odlika bioaktivnih stakala, koji su od neprocenjivog značaja za inženjerstvo koštanih tkiva.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Bioactive glasses in bone tissue engineering, Bioaktivna stakla u inženjerstvu koštanih tkiva",
volume = "62",
number = "2",
pages = "71-79",
doi = "10.1515/sdj-2015-0008"
}

Jokanović, V., Čolović, B., Marković, D.,& Živković, S.. (2015). Bioactive glasses in bone tissue engineering. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(2), 71-79.
https://doi.org/10.1515/sdj-2015-0008

Jokanović V, Čolović B, Marković D, Živković S. Bioactive glasses in bone tissue engineering. in Stomatološki glasnik Srbije. 2015;62(2):71-79.
doi:10.1515/sdj-2015-0008 .

Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Živković, Slavoljub, "Bioactive glasses in bone tissue engineering" in Stomatološki glasnik Srbije, 62, no. 2 (2015):71-79,
https://doi.org/10.1515/sdj-2015-0008 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Jokanović, Bojan
AU  - Živković, Slavoljub
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1990
AB  - The aim of this study was to synthesize and characterize new superplastic endodontic mixtures (named as ALBO-MPSA) with strongly accelerated hydration rate. Two different ALBO-MPSA mixtures were prepared using combination of hydrothermal and high-temperature self-propagating method. Phase analysis of the mixtures, before and after hydration, was carried out by X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM). Investigations of mechanical properties showed that new superplastic, quick-bonding endodontic mixtures with good mechanical properties were obtained (compressive strength after 28days aging was from 35.7 +/- 0.5 and 37.4 +/- 0.5 MPa). The final phase compositions after hydration of the mixtures were determined on the base of XRD data, assumed mechanism of hydration and rational chemical analysis. The constitutive phases were tobermorite, portlandite, and amorphous Ca(OH)(2), ettringite and amorphous like ettringite phase, gypsum, hydroxyapatite and barite.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Applied Ceramic Technology
T1  - Superplastic, Quick-Bonding Endodontic Mixtures and Their Hydration
VL  - 12
SP  - E83
EP  - E91
DO  - 10.1111/ijac.12234
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Jokanović, Bojan and Živković, Slavoljub",
year = "2015",
abstract = "The aim of this study was to synthesize and characterize new superplastic endodontic mixtures (named as ALBO-MPSA) with strongly accelerated hydration rate. Two different ALBO-MPSA mixtures were prepared using combination of hydrothermal and high-temperature self-propagating method. Phase analysis of the mixtures, before and after hydration, was carried out by X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM). Investigations of mechanical properties showed that new superplastic, quick-bonding endodontic mixtures with good mechanical properties were obtained (compressive strength after 28days aging was from 35.7 +/- 0.5 and 37.4 +/- 0.5 MPa). The final phase compositions after hydration of the mixtures were determined on the base of XRD data, assumed mechanism of hydration and rational chemical analysis. The constitutive phases were tobermorite, portlandite, and amorphous Ca(OH)(2), ettringite and amorphous like ettringite phase, gypsum, hydroxyapatite and barite.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Applied Ceramic Technology",
title = "Superplastic, Quick-Bonding Endodontic Mixtures and Their Hydration",
volume = "12",
pages = "E83-E91",
doi = "10.1111/ijac.12234"
}

Jokanović, V., Čolović, B., Jokanović, B.,& Živković, S.. (2015). Superplastic, Quick-Bonding Endodontic Mixtures and Their Hydration. in International Journal of Applied Ceramic Technology
Wiley-Blackwell, Hoboken., 12, E83-E91.
https://doi.org/10.1111/ijac.12234

Jokanović V, Čolović B, Jokanović B, Živković S. Superplastic, Quick-Bonding Endodontic Mixtures and Their Hydration. in International Journal of Applied Ceramic Technology. 2015;12:E83-E91.
doi:10.1111/ijac.12234 .

Jokanović, Vukoman, Čolović, Božana, Jokanović, Bojan, Živković, Slavoljub, "Superplastic, Quick-Bonding Endodontic Mixtures and Their Hydration" in International Journal of Applied Ceramic Technology, 12 (2015):E83-E91,
https://doi.org/10.1111/ijac.12234 . .

TY  - JOUR
AU  - Čolović, Božana
AU  - Marković, D.
AU  - Petrović, Milan
AU  - Jokanović, Vukoman
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1932
AB  - In this paper, the synthesis and characterization of calcium hydroxyapatite particles, stabilized with poly(ethylene-vinyl acetate) / poly(ethylene-vinyl versatate) (PEVA/PEVV) are presented. The particles were synthesized by two-step hydrothermal method using PEVA/PEVV as a surface-active substance. The structure and crystallinity of the obtained powder were investigated by X-ray diffraction, TEM and HRTEM. The phase identification of hydroxyapatite stabilized with PEVA/PEVV was done by FTIR, while the surface morphology, as well as the size and shape of particles were studied by TEM and AFM. Finally, using MTT and LDH release assays to assess mitochondrial activity and cell membrane integrity, it was confirmed that so synthesized hydroxyapatite powder was not toxic to L929 cells.
PB  - National Institute of Optoelectronics
T2  - Journal of Optoelectronics & Advanced Materials
T1  - Two-step hydrothermal synthesis of nano hydroxyapatite particles and their characterization
VL  - 16
IS  - 11-12
SP  - 1356
EP  - 1360
UR  - https://hdl.handle.net/21.15107/rcub_vinar_339
ER  - 

@article{
author = "Čolović, Božana and Marković, D. and Petrović, Milan and Jokanović, Vukoman",
year = "2014",
abstract = "In this paper, the synthesis and characterization of calcium hydroxyapatite particles, stabilized with poly(ethylene-vinyl acetate) / poly(ethylene-vinyl versatate) (PEVA/PEVV) are presented. The particles were synthesized by two-step hydrothermal method using PEVA/PEVV as a surface-active substance. The structure and crystallinity of the obtained powder were investigated by X-ray diffraction, TEM and HRTEM. The phase identification of hydroxyapatite stabilized with PEVA/PEVV was done by FTIR, while the surface morphology, as well as the size and shape of particles were studied by TEM and AFM. Finally, using MTT and LDH release assays to assess mitochondrial activity and cell membrane integrity, it was confirmed that so synthesized hydroxyapatite powder was not toxic to L929 cells.",
publisher = "National Institute of Optoelectronics",
journal = "Journal of Optoelectronics & Advanced Materials",
title = "Two-step hydrothermal synthesis of nano hydroxyapatite particles and their characterization",
volume = "16",
number = "11-12",
pages = "1356-1360",
url = "https://hdl.handle.net/21.15107/rcub_vinar_339"
}

Čolović, B., Marković, D., Petrović, M.,& Jokanović, V.. (2014). Two-step hydrothermal synthesis of nano hydroxyapatite particles and their characterization. in Journal of Optoelectronics & Advanced Materials
National Institute of Optoelectronics., 16(11-12), 1356-1360.
https://hdl.handle.net/21.15107/rcub_vinar_339

Čolović B, Marković D, Petrović M, Jokanović V. Two-step hydrothermal synthesis of nano hydroxyapatite particles and their characterization. in Journal of Optoelectronics & Advanced Materials. 2014;16(11-12):1356-1360.
https://hdl.handle.net/21.15107/rcub_vinar_339 .

Čolović, Božana, Marković, D., Petrović, Milan, Jokanović, Vukoman, "Two-step hydrothermal synthesis of nano hydroxyapatite particles and their characterization" in Journal of Optoelectronics & Advanced Materials, 16, no. 11-12 (2014):1356-1360,
https://hdl.handle.net/21.15107/rcub_vinar_339 .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Živković-Sandić, Marija
AU  - Petrović, Violeta
AU  - Živković, Slavoljub
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1867
AB  - Calcium phosphates, together with polymers, are most commonly used materials in bone engineering since their composition is similar to bone. They are used to fulfill various defects caused by injury or bone disease, as well as for the preparation of endodontic mixtures. Because of their great importance in dentistry, these materials are given special attention in the current paper. This paper is a part of the monograph entitled 'Nanomedicine, the Greatest Challenge of the 21st Century', which attracted great interest of technical and professional communities in different areas of medicine. Also for the last two years this book is promoted by the Student Cultural Centre as the only national book chosen in the narrowest election. That fact is very important for young researchers who study tissue engineering, endodontics and implantology.
AB  - Kalcijum- fosfati, zajedno s nekim polimerima, najviše su korišćeni materijali u inženjerstvu koštanog tkiva, budući da su po sastavu bliski prirodnoj kosti. Koriste se za ispune različitih oštećenja nastalih usled povreda ili bolesti koštanog tkiva, kao i za pripremu endodontskih mešavina za primenu u stomatologiji. Zbog izuzetnog značaja u stomatologiji, kalcijumfosfatni materijali zaslužuju posebno mesto, pa će im u okviru ovog rada, ali i radova koji će uslediti, biti posvećena posebna pažnja. Radovi su najvećim delom sastavni deo monografije pod nazivom 'Nanomedicina, najveći izazov 21. veka', koja je pobudila veliko interesovanje stručne i profesionalne javnosti usmerene ka različitim oblastima medicine i koju je već dve godine zaredom Studentski kulturni centar, kao jedinu knjigu domaćeg autora, promovisao kao knjigu najužeg izbora. Verujemo da je ta činjenica posebno važna za mlađe istraživače koji se bave problemima inženjerstva tkiva, endodoncijom i implantologijom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Calcium phosphate materials in bone tissue engineering
T1  - Kalcijumfosfatni materijali u inženjerstvu koštanog tkiva
VL  - 61
IS  - 2
SP  - 93
EP  - 101
DO  - 10.2298/sgs1402093j
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Živković-Sandić, Marija and Petrović, Violeta and Živković, Slavoljub",
year = "2014",
abstract = "Calcium phosphates, together with polymers, are most commonly used materials in bone engineering since their composition is similar to bone. They are used to fulfill various defects caused by injury or bone disease, as well as for the preparation of endodontic mixtures. Because of their great importance in dentistry, these materials are given special attention in the current paper. This paper is a part of the monograph entitled 'Nanomedicine, the Greatest Challenge of the 21st Century', which attracted great interest of technical and professional communities in different areas of medicine. Also for the last two years this book is promoted by the Student Cultural Centre as the only national book chosen in the narrowest election. That fact is very important for young researchers who study tissue engineering, endodontics and implantology., Kalcijum- fosfati, zajedno s nekim polimerima, najviše su korišćeni materijali u inženjerstvu koštanog tkiva, budući da su po sastavu bliski prirodnoj kosti. Koriste se za ispune različitih oštećenja nastalih usled povreda ili bolesti koštanog tkiva, kao i za pripremu endodontskih mešavina za primenu u stomatologiji. Zbog izuzetnog značaja u stomatologiji, kalcijumfosfatni materijali zaslužuju posebno mesto, pa će im u okviru ovog rada, ali i radova koji će uslediti, biti posvećena posebna pažnja. Radovi su najvećim delom sastavni deo monografije pod nazivom 'Nanomedicina, najveći izazov 21. veka', koja je pobudila veliko interesovanje stručne i profesionalne javnosti usmerene ka različitim oblastima medicine i koju je već dve godine zaredom Studentski kulturni centar, kao jedinu knjigu domaćeg autora, promovisao kao knjigu najužeg izbora. Verujemo da je ta činjenica posebno važna za mlađe istraživače koji se bave problemima inženjerstva tkiva, endodoncijom i implantologijom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Calcium phosphate materials in bone tissue engineering, Kalcijumfosfatni materijali u inženjerstvu koštanog tkiva",
volume = "61",
number = "2",
pages = "93-101",
doi = "10.2298/sgs1402093j"
}

Jokanović, V., Čolović, B., Živković-Sandić, M., Petrović, V.,& Živković, S.. (2014). Calcium phosphate materials in bone tissue engineering. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 61(2), 93-101.
https://doi.org/10.2298/sgs1402093j

Jokanović V, Čolović B, Živković-Sandić M, Petrović V, Živković S. Calcium phosphate materials in bone tissue engineering. in Stomatološki glasnik Srbije. 2014;61(2):93-101.
doi:10.2298/sgs1402093j .

Jokanović, Vukoman, Čolović, Božana, Živković-Sandić, Marija, Petrović, Violeta, Živković, Slavoljub, "Calcium phosphate materials in bone tissue engineering" in Stomatološki glasnik Srbije, 61, no. 2 (2014):93-101,
https://doi.org/10.2298/sgs1402093j . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Mitrić, Miodrag
AU  - Marković, Dejan
AU  - Ćetenović, Bojana
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1945
AB  - A synthesis of a new dental material, based on nano-structured highly active calcium silicates and calcium carbonates, is described in this paper. Phase analysis of this material, before and after hydration, was performed by X-ray diffraction and showed that active silicate phases were transformed into tobermorite phase, while carbonates remained unchanged. The mechanism of hydration was fully described. The morphology of the sample was studied by SEM, and typical appearance of the present phases was particularly discussed. The mechanical properties and setting time of this material make it very promising for potential application in dental practice.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Applied Ceramic Technology
T1  - Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates
VL  - 11
IS  - 1
SP  - 57
EP  - 64
DO  - 10.1111/ijac.12070
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Mitrić, Miodrag and Marković, Dejan and Ćetenović, Bojana",
year = "2014",
abstract = "A synthesis of a new dental material, based on nano-structured highly active calcium silicates and calcium carbonates, is described in this paper. Phase analysis of this material, before and after hydration, was performed by X-ray diffraction and showed that active silicate phases were transformed into tobermorite phase, while carbonates remained unchanged. The mechanism of hydration was fully described. The morphology of the sample was studied by SEM, and typical appearance of the present phases was particularly discussed. The mechanical properties and setting time of this material make it very promising for potential application in dental practice.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Applied Ceramic Technology",
title = "Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates",
volume = "11",
number = "1",
pages = "57-64",
doi = "10.1111/ijac.12070"
}

Jokanović, V., Čolović, B., Mitrić, M., Marković, D.,& Ćetenović, B.. (2014). Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates. in International Journal of Applied Ceramic Technology
Wiley-Blackwell, Hoboken., 11(1), 57-64.
https://doi.org/10.1111/ijac.12070

Jokanović V, Čolović B, Mitrić M, Marković D, Ćetenović B. Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates. in International Journal of Applied Ceramic Technology. 2014;11(1):57-64.
doi:10.1111/ijac.12070 .

Jokanović, Vukoman, Čolović, Božana, Mitrić, Miodrag, Marković, Dejan, Ćetenović, Bojana, "Synthesis and Properties of a New Dental Material Based on Nano-Structured Highly Active Calcium Silicates and Calcium Carbonates" in International Journal of Applied Ceramic Technology, 11, no. 1 (2014):57-64,
https://doi.org/10.1111/ijac.12070 . .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Živković, Slavoljub
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1786
AB  - Introduction. Mineral trioxide aggregate (MTA) is one of the most commonly used materials in endodontics. Given its shortcomings, there is an intensive search for new materials. Calcium-silicate phase (CS phase) is a new material synthesized by the method based on a combination of sol-gel process and self-propagation synthesis which can significantly improve setting time through accelerated hydration. The aim of this study was to explain the mechanisms of hardening of CS phase in aqueous medium as similar mechanism is expected in contact with body fluids. Materials and Methods. CS phases Ca3SiO5 (C3S) and 2β-CaSiO4 (β-C2S) were synthesized from CaCl2·5H2O (Merck, Germany). To investigate the process of hydration, CS were mixed with water and kept at 37 °C for 28 days in closed polyethylene containers. Analysis of the composition of samples before and after immersion in water for 1, 3, 7 and 28 days was performed using X - ray diffractometry and IR. Results. XRD patterns for hydrated samples during 1, 3, 7 and 28 days showed that the amount of hydrated tobermorite phases relative to the amount of CS phase changes with the time of hydration. After 1 day, in addition to the hydrated phases, a significant amount of untransformed β-C2S and C3S were found while after 28 days hydrated CS phase was completely transformed to tobermorite with a small amount of portlandite. Conclusion. Using combined method of sol-gel and self-propagating waves at high temperature very active nanostructured silicate phases were obtained. Hydration process of CS phases was analyzed using XRD and FTIR, the mechanism of hydration was proposed and it was pointed to the difficulties in determining the exact reaction as well as the problem of determining the exact structure of tobermorite.
AB  - Uvod. Mineral trioksidni agregat (MTA) je jedan od najčešće korišćenih materijala u endodonciji. S obzirom na njegove nedostatke, intenzivno se traga za novim materijalima. Kalcijum-silikatna faza (CS faza) je novi materijal sintetisan prema metodi zasnovanoj na kombinaciji sol-gel procesa i samoprenosivoj sintezi koja može značajno poboljšati vreme vezivanja kroz ubrzanu hidrataciju. Cilj ove studije je bio da objasni mehanizme otvrdnjavanja CS faze u vodenom medijumu budući da se sličan mehanizam očekuje u kontaktu s telesnim tečnostima. Materijal i metode rada. CS faze Ca3SiO5 (C3S) i 2β-CaSiO4 (β-C2S) su sintetisane iz CaCl2·5H2O (Merck, Nemačka). Da bismo istražili proces hidratacije, CS su pomešani s vodom i ostavljeni 28 dana na 37°C u zatvorenim polietilenskim kontejnerima. Analiza sastava uzoraka pre potapanja u vodu i jedan dan, tri, sedam i 28 dana nakon potapanja izvršena je difraktometrijom radioloških zraka i FTIR. Rezultati. XRD obrasci za uzorke hidratisane tokom jednog, tri, sedam i 28 dana pokazuju da se količina hidratisane faze tobermorita u odnosu na količinu faze CS menja s vremenom hidratacije. Kod uzorka hidratisanog jedan dan, pored hidratisane faze tobermorita, utvrđene su i značajne količine β-C2S i C3S koje su ostale netransformisane, da bi kod uzoraka hidratisanih 28 dana CS faza bila potpuno transformisana u tobermorit uz male količine portlandita. Zaključak. Pomoću kombinovane metode sol-gel i metode samopropagirajućih talasa na visokoj temperaturi dobijene su veoma aktivne nanostrukturne silikatne faze. Proces hidratacije CS faza je analiziran pomoću XRD i FTIR, predložen je mehanizam hidratacije i ukazano na teškoće u utvrđivanju tačne reakcije, kao i na problem utvrđivanja tačne strukture tobermorita.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Highly active calcium-silicate phases for application in endodontics
T1  - Primena vrlo aktivne kalcijum-silikatne faze u endodonciji
VL  - 60
IS  - 4
SP  - 210
EP  - 217
DO  - 10.2298/SGS1304210J
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Živković, Slavoljub",
year = "2013",
abstract = "Introduction. Mineral trioxide aggregate (MTA) is one of the most commonly used materials in endodontics. Given its shortcomings, there is an intensive search for new materials. Calcium-silicate phase (CS phase) is a new material synthesized by the method based on a combination of sol-gel process and self-propagation synthesis which can significantly improve setting time through accelerated hydration. The aim of this study was to explain the mechanisms of hardening of CS phase in aqueous medium as similar mechanism is expected in contact with body fluids. Materials and Methods. CS phases Ca3SiO5 (C3S) and 2β-CaSiO4 (β-C2S) were synthesized from CaCl2·5H2O (Merck, Germany). To investigate the process of hydration, CS were mixed with water and kept at 37 °C for 28 days in closed polyethylene containers. Analysis of the composition of samples before and after immersion in water for 1, 3, 7 and 28 days was performed using X - ray diffractometry and IR. Results. XRD patterns for hydrated samples during 1, 3, 7 and 28 days showed that the amount of hydrated tobermorite phases relative to the amount of CS phase changes with the time of hydration. After 1 day, in addition to the hydrated phases, a significant amount of untransformed β-C2S and C3S were found while after 28 days hydrated CS phase was completely transformed to tobermorite with a small amount of portlandite. Conclusion. Using combined method of sol-gel and self-propagating waves at high temperature very active nanostructured silicate phases were obtained. Hydration process of CS phases was analyzed using XRD and FTIR, the mechanism of hydration was proposed and it was pointed to the difficulties in determining the exact reaction as well as the problem of determining the exact structure of tobermorite., Uvod. Mineral trioksidni agregat (MTA) je jedan od najčešće korišćenih materijala u endodonciji. S obzirom na njegove nedostatke, intenzivno se traga za novim materijalima. Kalcijum-silikatna faza (CS faza) je novi materijal sintetisan prema metodi zasnovanoj na kombinaciji sol-gel procesa i samoprenosivoj sintezi koja može značajno poboljšati vreme vezivanja kroz ubrzanu hidrataciju. Cilj ove studije je bio da objasni mehanizme otvrdnjavanja CS faze u vodenom medijumu budući da se sličan mehanizam očekuje u kontaktu s telesnim tečnostima. Materijal i metode rada. CS faze Ca3SiO5 (C3S) i 2β-CaSiO4 (β-C2S) su sintetisane iz CaCl2·5H2O (Merck, Nemačka). Da bismo istražili proces hidratacije, CS su pomešani s vodom i ostavljeni 28 dana na 37°C u zatvorenim polietilenskim kontejnerima. Analiza sastava uzoraka pre potapanja u vodu i jedan dan, tri, sedam i 28 dana nakon potapanja izvršena je difraktometrijom radioloških zraka i FTIR. Rezultati. XRD obrasci za uzorke hidratisane tokom jednog, tri, sedam i 28 dana pokazuju da se količina hidratisane faze tobermorita u odnosu na količinu faze CS menja s vremenom hidratacije. Kod uzorka hidratisanog jedan dan, pored hidratisane faze tobermorita, utvrđene su i značajne količine β-C2S i C3S koje su ostale netransformisane, da bi kod uzoraka hidratisanih 28 dana CS faza bila potpuno transformisana u tobermorit uz male količine portlandita. Zaključak. Pomoću kombinovane metode sol-gel i metode samopropagirajućih talasa na visokoj temperaturi dobijene su veoma aktivne nanostrukturne silikatne faze. Proces hidratacije CS faza je analiziran pomoću XRD i FTIR, predložen je mehanizam hidratacije i ukazano na teškoće u utvrđivanju tačne reakcije, kao i na problem utvrđivanja tačne strukture tobermorita.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Highly active calcium-silicate phases for application in endodontics, Primena vrlo aktivne kalcijum-silikatne faze u endodonciji",
volume = "60",
number = "4",
pages = "210-217",
doi = "10.2298/SGS1304210J"
}

Jokanović, V., Čolović, B., Marković, D.,& Živković, S.. (2013). Highly active calcium-silicate phases for application in endodontics. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 60(4), 210-217.
https://doi.org/10.2298/SGS1304210J

Jokanović V, Čolović B, Marković D, Živković S. Highly active calcium-silicate phases for application in endodontics. in Stomatološki glasnik Srbije. 2013;60(4):210-217.
doi:10.2298/SGS1304210J .

Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Živković, Slavoljub, "Highly active calcium-silicate phases for application in endodontics" in Stomatološki glasnik Srbije, 60, no. 4 (2013):210-217,
https://doi.org/10.2298/SGS1304210J . .

TY  - JOUR
AU  - Petrović, Milan
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2012
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1692
AB  - The role of the process of designing a scaffold in bone tissue engineering is to provide optimal conditions for new bone tissue growth. The primary concern of such engineering is to create an adequate nanotopology of the scaffold inner walls, which can initiate the growth and activity of bone cells. Here, we present a completely new scaffold designing process based on a biomimetic approach in order to improve the nanostructure of pore walls of previously-made calcium hydroxyapatite (CHA) porous scaffolds. CHA porous scaffolds were covered with different polymer thin films (alginate, cellulose and PLGA) and exposed to simulated body fluid (SBF) for 42 days. SBF induced in situ formation of "bone-like" apatite phases on the surface of CHA/polymer composites. Fourier Transformed Infrared (FTIR) spectroscopy showed that the biomimetically-assembled phase is CHA of slightly shifted stoichiometry. X-ray diffraction confirmed that CHA is self-assembled on the surface of all investigated thin films. The calculation of crystallite sizes showed small differences in the degree of crystallinity between different samples. Scanning electron microscopy revealed a dominant blow-ball morphology of CHA particles (size 1 - 5 mu m) with nano-sized branches on their surfaces.
T2  - Journal of Ceramic Processing Research
T1  - Self assembly of biomimetic hydroxyapatite on the surface of different polymer thin films
VL  - 13
IS  - 4
SP  - 398
EP  - 404
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5031
ER  - 

@article{
author = "Petrović, Milan and Čolović, Božana and Jokanović, Vukoman and Marković, Dejan",
year = "2012",
abstract = "The role of the process of designing a scaffold in bone tissue engineering is to provide optimal conditions for new bone tissue growth. The primary concern of such engineering is to create an adequate nanotopology of the scaffold inner walls, which can initiate the growth and activity of bone cells. Here, we present a completely new scaffold designing process based on a biomimetic approach in order to improve the nanostructure of pore walls of previously-made calcium hydroxyapatite (CHA) porous scaffolds. CHA porous scaffolds were covered with different polymer thin films (alginate, cellulose and PLGA) and exposed to simulated body fluid (SBF) for 42 days. SBF induced in situ formation of "bone-like" apatite phases on the surface of CHA/polymer composites. Fourier Transformed Infrared (FTIR) spectroscopy showed that the biomimetically-assembled phase is CHA of slightly shifted stoichiometry. X-ray diffraction confirmed that CHA is self-assembled on the surface of all investigated thin films. The calculation of crystallite sizes showed small differences in the degree of crystallinity between different samples. Scanning electron microscopy revealed a dominant blow-ball morphology of CHA particles (size 1 - 5 mu m) with nano-sized branches on their surfaces.",
journal = "Journal of Ceramic Processing Research",
title = "Self assembly of biomimetic hydroxyapatite on the surface of different polymer thin films",
volume = "13",
number = "4",
pages = "398-404",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5031"
}

Petrović, M., Čolović, B., Jokanović, V.,& Marković, D.. (2012). Self assembly of biomimetic hydroxyapatite on the surface of different polymer thin films. in Journal of Ceramic Processing Research, 13(4), 398-404.
https://hdl.handle.net/21.15107/rcub_vinar_5031

Petrović M, Čolović B, Jokanović V, Marković D. Self assembly of biomimetic hydroxyapatite on the surface of different polymer thin films. in Journal of Ceramic Processing Research. 2012;13(4):398-404.
https://hdl.handle.net/21.15107/rcub_vinar_5031 .

Petrović, Milan, Čolović, Božana, Jokanović, Vukoman, Marković, Dejan, "Self assembly of biomimetic hydroxyapatite on the surface of different polymer thin films" in Journal of Ceramic Processing Research, 13, no. 4 (2012):398-404,
https://hdl.handle.net/21.15107/rcub_vinar_5031 .

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Živković, Slavoljub
AU  - Živojinović, Vesna
AU  - Marković, Dejan
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1673
AB  - Mineral trioxide aggregate (MTA) is a material with broad indications in endodontics. Currently, this material is used for pulp capping, pulpotomy, as apical barrier in teeth with necrotic pulp and open apex, and in the treatment of various root canal perforations. By comparing the biological response of pulp tissue capped with calcium hydroxide and MTA, the superiority of MTA has been indicated. The bridge of hard tissue formed under MTA prevented the irritation of pulp. Histological studies of pulpotomy in dog teeth have shown after 120 days a hard tissue without necrosis formed under MTA. MTA is also the material of choice for retrograde root canal obturation and lateral perforation. Meta analysis of published papers on MTA in recent years indicated the great clinical success of this material which ensured a good canal seal and superior biocompatibility. It is the only material for retrograde root canal obturation that encourages tissue regeneration. Studies have also shown that MTA has inductive and conductive properties for hard tissue as well as the ability to release ions in a liquid medium. The main disadvantage of MTA is related to its color, the presence of iron and mangan ions, the difficulties in maintaining the characteristics of material during time, absence of solvent for this material and the difficulty for its removal after crosslinking.
AB  - Mineral trioksid-agregat (MTA) je materijal koji ima široke indikacije u endodonciji. Danas se koristi kod prekrivanja vitalne pulpe i pulpotomije, kao apeksna barijera kod zuba s nekrotičnom pulpom i otvorenim apeksom korena, odnosno u lečenju različitih perforacija kanala korena. Poređenja biološkog odgovora tkiva pulpe prekrivene kalcijum-hidroksidom i MTA ukazuju na superiornost MTA, jer se formira most tvrdog tkiva koji sprečava iritaciju pulpe. Histološke studije pulpotomija na zubima pasa pokazaLe su da se ispod MTA stvara tvrdo koštano tkivo bez nekroze posle 120 dana. MTA je takođe materijal izbora za retrogradno punjenje kanala korena, odnosno za terapiju lateralnih perforacija. Metaanaliza publikovanih radova o MTA poslednjih godina ukazuje na veliki klinički uspeh ovog materijala, koji obezbeđuje dobro zaptivanje, pokazuje superiornu biokompatibilnost i jedini je materijal za retrogradno punjenje kanala korena zuba koji pospešuje regeneraciju tkiva. Istraživanja takođe pokazuju da MTA ima induktivna i konduktivna svojstva za formiranje tvrdog tkiva, kao i osobinu otpuštanja različitih jona kada se nalazi u tečnoj sredini. Glavni nedostatak MTA je u vezi sa bojom zbog prisustva gvožđa i mangana, sa teškoćama održavanja osobina materijala tokom vremena, sa izostankom rastvarača za ovaj materijal i sa teškoćama njegovog uklanjanja posle umrežavanja.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Mineral trioxide aggregate as material of choice in endodontic therapy
T1  - Mineral trioksid-agregat kao materijal izbora u endodonciji
VL  - 58
IS  - 2
SP  - 97
EP  - 107
DO  - 10.2298/SGS1102097J
ER  - 

@article{
author = "Jokanović, Vukoman and Čolović, Božana and Živković, Slavoljub and Živojinović, Vesna and Marković, Dejan",
year = "2011",
abstract = "Mineral trioxide aggregate (MTA) is a material with broad indications in endodontics. Currently, this material is used for pulp capping, pulpotomy, as apical barrier in teeth with necrotic pulp and open apex, and in the treatment of various root canal perforations. By comparing the biological response of pulp tissue capped with calcium hydroxide and MTA, the superiority of MTA has been indicated. The bridge of hard tissue formed under MTA prevented the irritation of pulp. Histological studies of pulpotomy in dog teeth have shown after 120 days a hard tissue without necrosis formed under MTA. MTA is also the material of choice for retrograde root canal obturation and lateral perforation. Meta analysis of published papers on MTA in recent years indicated the great clinical success of this material which ensured a good canal seal and superior biocompatibility. It is the only material for retrograde root canal obturation that encourages tissue regeneration. Studies have also shown that MTA has inductive and conductive properties for hard tissue as well as the ability to release ions in a liquid medium. The main disadvantage of MTA is related to its color, the presence of iron and mangan ions, the difficulties in maintaining the characteristics of material during time, absence of solvent for this material and the difficulty for its removal after crosslinking., Mineral trioksid-agregat (MTA) je materijal koji ima široke indikacije u endodonciji. Danas se koristi kod prekrivanja vitalne pulpe i pulpotomije, kao apeksna barijera kod zuba s nekrotičnom pulpom i otvorenim apeksom korena, odnosno u lečenju različitih perforacija kanala korena. Poređenja biološkog odgovora tkiva pulpe prekrivene kalcijum-hidroksidom i MTA ukazuju na superiornost MTA, jer se formira most tvrdog tkiva koji sprečava iritaciju pulpe. Histološke studije pulpotomija na zubima pasa pokazaLe su da se ispod MTA stvara tvrdo koštano tkivo bez nekroze posle 120 dana. MTA je takođe materijal izbora za retrogradno punjenje kanala korena, odnosno za terapiju lateralnih perforacija. Metaanaliza publikovanih radova o MTA poslednjih godina ukazuje na veliki klinički uspeh ovog materijala, koji obezbeđuje dobro zaptivanje, pokazuje superiornu biokompatibilnost i jedini je materijal za retrogradno punjenje kanala korena zuba koji pospešuje regeneraciju tkiva. Istraživanja takođe pokazuju da MTA ima induktivna i konduktivna svojstva za formiranje tvrdog tkiva, kao i osobinu otpuštanja različitih jona kada se nalazi u tečnoj sredini. Glavni nedostatak MTA je u vezi sa bojom zbog prisustva gvožđa i mangana, sa teškoćama održavanja osobina materijala tokom vremena, sa izostankom rastvarača za ovaj materijal i sa teškoćama njegovog uklanjanja posle umrežavanja.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Mineral trioxide aggregate as material of choice in endodontic therapy, Mineral trioksid-agregat kao materijal izbora u endodonciji",
volume = "58",
number = "2",
pages = "97-107",
doi = "10.2298/SGS1102097J"
}

Jokanović, V., Čolović, B., Živković, S., Živojinović, V.,& Marković, D.. (2011). Mineral trioxide aggregate as material of choice in endodontic therapy. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 58(2), 97-107.
https://doi.org/10.2298/SGS1102097J

Jokanović V, Čolović B, Živković S, Živojinović V, Marković D. Mineral trioxide aggregate as material of choice in endodontic therapy. in Stomatološki glasnik Srbije. 2011;58(2):97-107.
doi:10.2298/SGS1102097J .

Jokanović, Vukoman, Čolović, Božana, Živković, Slavoljub, Živojinović, Vesna, Marković, Dejan, "Mineral trioxide aggregate as material of choice in endodontic therapy" in Stomatološki glasnik Srbije, 58, no. 2 (2011):97-107,
https://doi.org/10.2298/SGS1102097J . .

TY  - JOUR
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Jokanović, Vukoman
PY  - 2011
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1633
AB  - Introduction. The aim of the study was to assess the formation of biomimetic calcium hydroxyapatite (HAP) on the surface of different substrates. Material and Methods. Silica coated stainless steel tapes and thin polymer films (alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited on hydroxyapatite scaffold were used as substrate. Supersaturated simulated body fluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle's Minimum Essential Medium (EMEM) were used as bioactive liquid medium where biomimetic nucleation of HAP occurred. Infrared spectroscopy with Fourier transformation was used to analyze the formed phases, while scanning electron microscopy indicated the morphology of nucleated phase. Results. The results of measuring the mass with volume adjustments done by the BET method showed that the thickness of the film of nucleated calcium hydroxyapatite depended on the time that samples spent soaked in SBF-in as well as the type of selected biomimetic medium. Conclusion. Biomimetic calcium hydroxyapatite is possible to produce by self nucleation on different substrates in the presence of simulating body fluid.
AB  - Uvod. Cilj ovog rada je bio da se ispita formiranje biomimetskog kalcijum-hidroksiapatita na površinama različitih supstrata. Materijal i metode rada. Kao supstrati su korišćeni tanki filmovi silicijum-dioksida naneti na čelične trake i tanki polimerni filmovi (alginat, celuloza, polilaktidkoglikolid) naneti na visokoporozni hidroksiapatitni nosač. Simulirana telesna tečnost (engl. simulated body fluid-SBF), kao i SBF kombinovan sa serumom iz fetusa govečeta ili Iglovim medijumom korišćeni su kao bioaktivni tečni medijum u kojem se odvija biomimetska nukleacija apatita. Za analizu formiranih faza korišćena je infracrvena spektroskopija sa Furijeovom transformacijom, dok je skening elektronska mikroskopija ukazivala na morfologiju nukleirane faze. Rezultati. Merenje mase uz korekciju zapremine primenom metode BET pokazalo je da debljina filma nukleiranog kalcijum-hidroksiapatita zavisi od vremena držanja uzoraka u SBF, kao i od vrste izabranog biomimetskog medijuma. Zaključak. Dobijanje biomimetskog kalcijum-hidroksiapatita moguće je samonukleacijom na različitim supstratima i primenom SBF.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Nucleation of biomimetic hydroxyapatite
T1  - Nukleacija biomimetskog hidroksiapatita
VL  - 58
IS  - 1
SP  - 7
EP  - 15
DO  - 10.2298/SGS1101007C
ER  - 

@article{
author = "Čolović, Božana and Marković, Dejan and Jokanović, Vukoman",
year = "2011",
abstract = "Introduction. The aim of the study was to assess the formation of biomimetic calcium hydroxyapatite (HAP) on the surface of different substrates. Material and Methods. Silica coated stainless steel tapes and thin polymer films (alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited on hydroxyapatite scaffold were used as substrate. Supersaturated simulated body fluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle's Minimum Essential Medium (EMEM) were used as bioactive liquid medium where biomimetic nucleation of HAP occurred. Infrared spectroscopy with Fourier transformation was used to analyze the formed phases, while scanning electron microscopy indicated the morphology of nucleated phase. Results. The results of measuring the mass with volume adjustments done by the BET method showed that the thickness of the film of nucleated calcium hydroxyapatite depended on the time that samples spent soaked in SBF-in as well as the type of selected biomimetic medium. Conclusion. Biomimetic calcium hydroxyapatite is possible to produce by self nucleation on different substrates in the presence of simulating body fluid., Uvod. Cilj ovog rada je bio da se ispita formiranje biomimetskog kalcijum-hidroksiapatita na površinama različitih supstrata. Materijal i metode rada. Kao supstrati su korišćeni tanki filmovi silicijum-dioksida naneti na čelične trake i tanki polimerni filmovi (alginat, celuloza, polilaktidkoglikolid) naneti na visokoporozni hidroksiapatitni nosač. Simulirana telesna tečnost (engl. simulated body fluid-SBF), kao i SBF kombinovan sa serumom iz fetusa govečeta ili Iglovim medijumom korišćeni su kao bioaktivni tečni medijum u kojem se odvija biomimetska nukleacija apatita. Za analizu formiranih faza korišćena je infracrvena spektroskopija sa Furijeovom transformacijom, dok je skening elektronska mikroskopija ukazivala na morfologiju nukleirane faze. Rezultati. Merenje mase uz korekciju zapremine primenom metode BET pokazalo je da debljina filma nukleiranog kalcijum-hidroksiapatita zavisi od vremena držanja uzoraka u SBF, kao i od vrste izabranog biomimetskog medijuma. Zaključak. Dobijanje biomimetskog kalcijum-hidroksiapatita moguće je samonukleacijom na različitim supstratima i primenom SBF.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Nucleation of biomimetic hydroxyapatite, Nukleacija biomimetskog hidroksiapatita",
volume = "58",
number = "1",
pages = "7-15",
doi = "10.2298/SGS1101007C"
}

Čolović, B., Marković, D.,& Jokanović, V.. (2011). Nucleation of biomimetic hydroxyapatite. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 58(1), 7-15.
https://doi.org/10.2298/SGS1101007C

Čolović B, Marković D, Jokanović V. Nucleation of biomimetic hydroxyapatite. in Stomatološki glasnik Srbije. 2011;58(1):7-15.
doi:10.2298/SGS1101007C .

Čolović, Božana, Marković, Dejan, Jokanović, Vukoman, "Nucleation of biomimetic hydroxyapatite" in Stomatološki glasnik Srbije, 58, no. 1 (2011):7-15,
https://doi.org/10.2298/SGS1101007C . .

TY  - JOUR
AU  - Čolović, Božana
AU  - Todorović-Marković, B.
AU  - Marković, Z.
AU  - Marković, Dejan
AU  - Plavsić, M.
AU  - Jokanović, Vukoman
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1561
AB  - Calcium hydroxyapatite (CHA) formation was produced by biomimetic deposition on the surfaces of silica layers of the steel bands, using slightly modified simulated body fluid (SBF). The used SBF had a pH value of 7.4 and a similar ionic composition like human plasma. Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) method was used to analyse obtained phases on the surfaces of silica films after various times of sample soaking in SBF. Atomic force microscopy (AFM) investigations pointed out the mechanism of CHA nucleation. Mass changes investigations of the CHA thin films showed that the depth of CHA films was dependent on the aging time of silica substrate in SBF.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Surface Engineering
T1  - Nucleation of calcium hydroxyapatite thin films from simulated body fluid
VL  - 26
IS  - 7
SP  - 532
EP  - 535
DO  - 10.1179/174329409X439005
ER  - 

@article{
author = "Čolović, Božana and Todorović-Marković, B. and Marković, Z. and Marković, Dejan and Plavsić, M. and Jokanović, Vukoman",
year = "2010",
abstract = "Calcium hydroxyapatite (CHA) formation was produced by biomimetic deposition on the surfaces of silica layers of the steel bands, using slightly modified simulated body fluid (SBF). The used SBF had a pH value of 7.4 and a similar ionic composition like human plasma. Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) method was used to analyse obtained phases on the surfaces of silica films after various times of sample soaking in SBF. Atomic force microscopy (AFM) investigations pointed out the mechanism of CHA nucleation. Mass changes investigations of the CHA thin films showed that the depth of CHA films was dependent on the aging time of silica substrate in SBF.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Surface Engineering",
title = "Nucleation of calcium hydroxyapatite thin films from simulated body fluid",
volume = "26",
number = "7",
pages = "532-535",
doi = "10.1179/174329409X439005"
}

Čolović, B., Todorović-Marković, B., Marković, Z., Marković, D., Plavsić, M.,& Jokanović, V.. (2010). Nucleation of calcium hydroxyapatite thin films from simulated body fluid. in Surface Engineering
Taylor & Francis Ltd, Abingdon., 26(7), 532-535.
https://doi.org/10.1179/174329409X439005

Čolović B, Todorović-Marković B, Marković Z, Marković D, Plavsić M, Jokanović V. Nucleation of calcium hydroxyapatite thin films from simulated body fluid. in Surface Engineering. 2010;26(7):532-535.
doi:10.1179/174329409X439005 .

Čolović, Božana, Todorović-Marković, B., Marković, Z., Marković, Dejan, Plavsić, M., Jokanović, Vukoman, "Nucleation of calcium hydroxyapatite thin films from simulated body fluid" in Surface Engineering, 26, no. 7 (2010):532-535,
https://doi.org/10.1179/174329409X439005 . .