TY  - JOUR
AU  - Milašin, Jelena
AU  - Buffo, Annalisa
AU  - Carulli, Daniela
AU  - Strata, Piergiorgio
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1351
AB  - Subtotal lesion of the inferior olive (IO) achieved by treating experimental animals with 3-acetylpyridine (3AP) induces partial Purkinje cells (PCs) deafferentation that leads to PC hyperactivity and new spine formation. Coincidentally, the olivary terminals belonging to the few survived olivary neurons undergo an extensive collateral sprouting resulting in reinnervation of the neighboring denervated PCs. We obtained chemical deafferentation of PCs in adult rats (body weight, 120-170 g; age, 35-40 days) by a single intraperitoneal injection of 3AP (65 mg/kg body weight), and as early as 3 days after 3AP treatment, important morphological changes could be observed on PCs. Mitogen-activated protein kinase (MAPK) cascades and more specifically extracellular signal-regulated kinases 1/2 (ERK1/2) play a critical role in the signaling events underlying synaptic plasticity. For instance, long-term depression (LTD) in the adult hippocampus and long-term potentiation (LTP) in cerebellum both involve ERK activation. Since PCs deprived of their climbing fibers (CFs) afferents initiate an intensive remodeling of the spines and rapid recall of the remaining CFs, it prompted us to see whether the observed phenomena correlated with MAPK and Akt activation. Immunohistochemistry and Western blotting were done at various time points after 3AP application (from 24 h to 6 days), as the exact dynamics of CF loss is not precisely known. As judged by Western blotting, there was no increase of activated ERK in the cerebellum. However, immunohistochemistry revealed increased ERK phosphorylation in the "pinceaux" of basket cells in 3AP animals. Similarly, stress-activated protein kinase(SAPK)/c-Jun N-terminal kinase (JNK), p38 MAPK, and Akt activation were also studied by means of Western blotting and immunohistochemistry. Upon 3AP treatment no changes in phosphorylation status could be seen in the different kinases subjected to analysis. Our results suggest that activation of MAPK and Akt cascades is not essential in this model of neuronal plasticity.
PB  - Blackwell Publishing, Oxford
T2  - Signal Transduction Pathways, Pt D: Inflammatory Signaling Pathways & Neuropathology
T1  - Intensive remodeling of Purkinje cell spines after climbing fibers deafferentation does not involve MAPK and Akt activation
VL  - 1096
SP  - 230
EP  - 238
DO  - 10.1196/annals.1397.089
ER  - 

@article{
author = "Milašin, Jelena and Buffo, Annalisa and Carulli, Daniela and Strata, Piergiorgio",
year = "2007",
abstract = "Subtotal lesion of the inferior olive (IO) achieved by treating experimental animals with 3-acetylpyridine (3AP) induces partial Purkinje cells (PCs) deafferentation that leads to PC hyperactivity and new spine formation. Coincidentally, the olivary terminals belonging to the few survived olivary neurons undergo an extensive collateral sprouting resulting in reinnervation of the neighboring denervated PCs. We obtained chemical deafferentation of PCs in adult rats (body weight, 120-170 g; age, 35-40 days) by a single intraperitoneal injection of 3AP (65 mg/kg body weight), and as early as 3 days after 3AP treatment, important morphological changes could be observed on PCs. Mitogen-activated protein kinase (MAPK) cascades and more specifically extracellular signal-regulated kinases 1/2 (ERK1/2) play a critical role in the signaling events underlying synaptic plasticity. For instance, long-term depression (LTD) in the adult hippocampus and long-term potentiation (LTP) in cerebellum both involve ERK activation. Since PCs deprived of their climbing fibers (CFs) afferents initiate an intensive remodeling of the spines and rapid recall of the remaining CFs, it prompted us to see whether the observed phenomena correlated with MAPK and Akt activation. Immunohistochemistry and Western blotting were done at various time points after 3AP application (from 24 h to 6 days), as the exact dynamics of CF loss is not precisely known. As judged by Western blotting, there was no increase of activated ERK in the cerebellum. However, immunohistochemistry revealed increased ERK phosphorylation in the "pinceaux" of basket cells in 3AP animals. Similarly, stress-activated protein kinase(SAPK)/c-Jun N-terminal kinase (JNK), p38 MAPK, and Akt activation were also studied by means of Western blotting and immunohistochemistry. Upon 3AP treatment no changes in phosphorylation status could be seen in the different kinases subjected to analysis. Our results suggest that activation of MAPK and Akt cascades is not essential in this model of neuronal plasticity.",
publisher = "Blackwell Publishing, Oxford",
journal = "Signal Transduction Pathways, Pt D: Inflammatory Signaling Pathways & Neuropathology",
title = "Intensive remodeling of Purkinje cell spines after climbing fibers deafferentation does not involve MAPK and Akt activation",
volume = "1096",
pages = "230-238",
doi = "10.1196/annals.1397.089"
}

Milašin, J., Buffo, A., Carulli, D.,& Strata, P.. (2007). Intensive remodeling of Purkinje cell spines after climbing fibers deafferentation does not involve MAPK and Akt activation. in Signal Transduction Pathways, Pt D: Inflammatory Signaling Pathways & Neuropathology
Blackwell Publishing, Oxford., 1096, 230-238.
https://doi.org/10.1196/annals.1397.089

Milašin J, Buffo A, Carulli D, Strata P. Intensive remodeling of Purkinje cell spines after climbing fibers deafferentation does not involve MAPK and Akt activation. in Signal Transduction Pathways, Pt D: Inflammatory Signaling Pathways & Neuropathology. 2007;1096:230-238.
doi:10.1196/annals.1397.089 .

Milašin, Jelena, Buffo, Annalisa, Carulli, Daniela, Strata, Piergiorgio, "Intensive remodeling of Purkinje cell spines after climbing fibers deafferentation does not involve MAPK and Akt activation" in Signal Transduction Pathways, Pt D: Inflammatory Signaling Pathways & Neuropathology, 1096 (2007):230-238,
https://doi.org/10.1196/annals.1397.089 . .

TY  - JOUR
AU  - Milašin, Jelena
AU  - Buffo, Annalisa
AU  - Carulli, Daniela
AU  - Andjus, P
AU  - Strata, Piergiorgio
PY  - 2005
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1245
AB  - The mitogen-activated protein kinases (MAPKs) are a family of signal transduction mediators that regulate a number of cellular activities, including cell growth and proliferation, differentiation and survival, via phosphorylation (activation) of protein kinases. MAPKs are also recruited during synaptic plasticity and remodeling. In the present study we used Western blotting and immunohistochemistry to examine the effects of harmaline administration on the phosphorylation state of three MAPKs: the extracellular signalregulated kinase (ERK1/2), c-jun-N-terminal kinase/stress-activated protein kinase (JNK/SAPK), and p38 MAPK. Harmaline is a tremorigenic drug known to induce enhanced and rhythmic firing of the inferior olive. In rats, synchronous activity of the inferior olive cells induced by harmaline administered for four days from postnatal day 9 to 12 resulted in prolonged maintenance of polyinnervation of Purkinje cells by climbing fibers (axons of olivary cells). Immunohistochemistry showed small but sustained cytoplasmic positivity to phospho-ERK in Purkinje cells and a strong signal for phospho-ERK in the "pinceaux," terminals of the interneuronal basket cells onto Purkinje cells. A similar pattern was observed for JNK/SAPK, while no changes in p38 were noticed. Thus, it was revealed that the activation of two members of the MAPK family in these inhibitory presynaptic terminals is also one consequence of synchronous olivary input to Purkinje cells known to affect developmental plasticity.
PB  - New York Acad Sciences, New York
T2  - Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - MAPK activation in cerebellar basket cell terminals after harmaline treatment
VL  - 1048
SP  - 411
EP  - 417
DO  - 10.1196/annals.1342.051
ER  - 

@article{
author = "Milašin, Jelena and Buffo, Annalisa and Carulli, Daniela and Andjus, P and Strata, Piergiorgio",
year = "2005",
abstract = "The mitogen-activated protein kinases (MAPKs) are a family of signal transduction mediators that regulate a number of cellular activities, including cell growth and proliferation, differentiation and survival, via phosphorylation (activation) of protein kinases. MAPKs are also recruited during synaptic plasticity and remodeling. In the present study we used Western blotting and immunohistochemistry to examine the effects of harmaline administration on the phosphorylation state of three MAPKs: the extracellular signalregulated kinase (ERK1/2), c-jun-N-terminal kinase/stress-activated protein kinase (JNK/SAPK), and p38 MAPK. Harmaline is a tremorigenic drug known to induce enhanced and rhythmic firing of the inferior olive. In rats, synchronous activity of the inferior olive cells induced by harmaline administered for four days from postnatal day 9 to 12 resulted in prolonged maintenance of polyinnervation of Purkinje cells by climbing fibers (axons of olivary cells). Immunohistochemistry showed small but sustained cytoplasmic positivity to phospho-ERK in Purkinje cells and a strong signal for phospho-ERK in the "pinceaux," terminals of the interneuronal basket cells onto Purkinje cells. A similar pattern was observed for JNK/SAPK, while no changes in p38 were noticed. Thus, it was revealed that the activation of two members of the MAPK family in these inhibitory presynaptic terminals is also one consequence of synchronous olivary input to Purkinje cells known to affect developmental plasticity.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "MAPK activation in cerebellar basket cell terminals after harmaline treatment",
volume = "1048",
pages = "411-417",
doi = "10.1196/annals.1342.051"
}

Milašin, J., Buffo, A., Carulli, D., Andjus, P.,& Strata, P.. (2005). MAPK activation in cerebellar basket cell terminals after harmaline treatment. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 411-417.
https://doi.org/10.1196/annals.1342.051

Milašin J, Buffo A, Carulli D, Andjus P, Strata P. MAPK activation in cerebellar basket cell terminals after harmaline treatment. in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:411-417.
doi:10.1196/annals.1342.051 .

Milašin, Jelena, Buffo, Annalisa, Carulli, Daniela, Andjus, P, Strata, Piergiorgio, "MAPK activation in cerebellar basket cell terminals after harmaline treatment" in Biophysics from Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):411-417,
https://doi.org/10.1196/annals.1342.051 . .