TY  - JOUR
AU  - Petrović, Vanja
AU  - Danilović, Vesna
AU  - Marković, Dejan
AU  - Čakić, Saša
AU  - Krstić, N.
AU  - Marković, Danica
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1479
AB  - There are very few data about the effects of endogenous growth factors in vital pulp therapy, and still they are often controversial. This study was undertaken with the aim to evaluate the effects of platelet rich plasma (PRP) in conjugation with hydroxyapatite (HAP), as pulp capping materials, on root and periodontium formation. Eight young monkeys (Cercopithecus Aethiops) with permanent dentition and incomplete root formation were involved in this study. After pulpotomy, the pulp lesion was capped with calcium hydroxide (control), HAP (experimental) or HAP in conjugation with PRP (experimental). Six moths later, the animals were sacrificed, the tissue was removed en block, and prepared for histological analysis in a routine way. The results of histological analysis revealed that the healing process, characterized by dentin bridge formation, maintained morphological and functional integrity of dental pulp and complete formation of dental root and surrounding periodontium. The inflammatory reaction was scored as mild to moderate, in almost all samples in all groups, suggesting the biocompatibility of the used materials. Materials used in this study are convenient as capping agents, contributing in maintaining the integrity of the pulp tissue and facilitating root and periodontium formation. According to histological data it could be suggested that hydroxyapatite in conjugation with endogenous growth factors, represent a superior alternative to other materials used in this study.
AB  - Podaci iz literature o primeni faktora rasta u vitalnoj terapiji pulpe su oskudni i često kontraverzni. Cilj našeg rada je bio da se ispita uticaj plazme bogate trombocitima u kombinaciji sa hidroksiapatitom, kao materijala za direktno prekrivanje pulpe, na završetak rasta korena i formiranje okolnog parodoncijuma. Istraživanje je sprovedeno na osam mladih majmuna (Cercopithecus aethiops), sa stalnom denticijom i nezavršenim rastom korena. Nakon pulpotomije, pulpna lezija je prekrivena kalcijum hidroksidom (kontolna grupa), hidroksiapatitom (eksperimentalna grupa I) ili hidroksiapatitom u kombinaciji sa plazmom bogatom trombocitima (eksperimentalna grupa II). Šest meseci kasnije životinje su žrtvovane, a uzeto tkivo pripremljeno je za histološku analizu. Rezultati histološke analize ukazali su da je zarastanje bilo karakterisano stvaranjem mineralizovanog dentinskog mostića, uz očuvanje morfološkog i funkcionalnog integriteta pulpe i završetak rasta korena i okolnog parodoncijuma u uzorcima svih ispitivanih grupa. Zapaljenska reakcija je gotovo u svim ispitivnim uzorcima bila ocenjena kao blaga ili umerena, što govori u prilog biokompatibilnosti primenjenih materijala. Na osnovu dobijenih rezultata može se reći da su svi primenjeni materijali pogodni za direktno prekrivanje pulpe, da doprinose očuvanju integriteta pulpe i ubrzavaju završetak rasta korena i formiranje apikalnog parodoncijuma, s tim što hidroksipatit u kombinaciji sa endogenim faktorima rasta predstavlja superiorniju alternativu u odnosu na druge materijale korišćene u ovoj studiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effects of hydroxyapatite and platelet rich plasma on apexogenesis in monkeys
T1  - Uticaj hidroksiapatita i plazme bogate trombocitima na proces apeksogeneze kod majmuna
VL  - 59
IS  - 2-3
SP  - 277
EP  - 289
DO  - 10.2298/AVB0903277P
ER  - 

@article{
author = "Petrović, Vanja and Danilović, Vesna and Marković, Dejan and Čakić, Saša and Krstić, N. and Marković, Danica",
year = "2009",
abstract = "There are very few data about the effects of endogenous growth factors in vital pulp therapy, and still they are often controversial. This study was undertaken with the aim to evaluate the effects of platelet rich plasma (PRP) in conjugation with hydroxyapatite (HAP), as pulp capping materials, on root and periodontium formation. Eight young monkeys (Cercopithecus Aethiops) with permanent dentition and incomplete root formation were involved in this study. After pulpotomy, the pulp lesion was capped with calcium hydroxide (control), HAP (experimental) or HAP in conjugation with PRP (experimental). Six moths later, the animals were sacrificed, the tissue was removed en block, and prepared for histological analysis in a routine way. The results of histological analysis revealed that the healing process, characterized by dentin bridge formation, maintained morphological and functional integrity of dental pulp and complete formation of dental root and surrounding periodontium. The inflammatory reaction was scored as mild to moderate, in almost all samples in all groups, suggesting the biocompatibility of the used materials. Materials used in this study are convenient as capping agents, contributing in maintaining the integrity of the pulp tissue and facilitating root and periodontium formation. According to histological data it could be suggested that hydroxyapatite in conjugation with endogenous growth factors, represent a superior alternative to other materials used in this study., Podaci iz literature o primeni faktora rasta u vitalnoj terapiji pulpe su oskudni i često kontraverzni. Cilj našeg rada je bio da se ispita uticaj plazme bogate trombocitima u kombinaciji sa hidroksiapatitom, kao materijala za direktno prekrivanje pulpe, na završetak rasta korena i formiranje okolnog parodoncijuma. Istraživanje je sprovedeno na osam mladih majmuna (Cercopithecus aethiops), sa stalnom denticijom i nezavršenim rastom korena. Nakon pulpotomije, pulpna lezija je prekrivena kalcijum hidroksidom (kontolna grupa), hidroksiapatitom (eksperimentalna grupa I) ili hidroksiapatitom u kombinaciji sa plazmom bogatom trombocitima (eksperimentalna grupa II). Šest meseci kasnije životinje su žrtvovane, a uzeto tkivo pripremljeno je za histološku analizu. Rezultati histološke analize ukazali su da je zarastanje bilo karakterisano stvaranjem mineralizovanog dentinskog mostića, uz očuvanje morfološkog i funkcionalnog integriteta pulpe i završetak rasta korena i okolnog parodoncijuma u uzorcima svih ispitivanih grupa. Zapaljenska reakcija je gotovo u svim ispitivnim uzorcima bila ocenjena kao blaga ili umerena, što govori u prilog biokompatibilnosti primenjenih materijala. Na osnovu dobijenih rezultata može se reći da su svi primenjeni materijali pogodni za direktno prekrivanje pulpe, da doprinose očuvanju integriteta pulpe i ubrzavaju završetak rasta korena i formiranje apikalnog parodoncijuma, s tim što hidroksipatit u kombinaciji sa endogenim faktorima rasta predstavlja superiorniju alternativu u odnosu na druge materijale korišćene u ovoj studiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effects of hydroxyapatite and platelet rich plasma on apexogenesis in monkeys, Uticaj hidroksiapatita i plazme bogate trombocitima na proces apeksogeneze kod majmuna",
volume = "59",
number = "2-3",
pages = "277-289",
doi = "10.2298/AVB0903277P"
}

Petrović, V., Danilović, V., Marković, D., Čakić, S., Krstić, N.,& Marković, D.. (2009). The effects of hydroxyapatite and platelet rich plasma on apexogenesis in monkeys. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 277-289.
https://doi.org/10.2298/AVB0903277P

Petrović V, Danilović V, Marković D, Čakić S, Krstić N, Marković D. The effects of hydroxyapatite and platelet rich plasma on apexogenesis in monkeys. in Acta veterinaria. 2009;59(2-3):277-289.
doi:10.2298/AVB0903277P .

Petrović, Vanja, Danilović, Vesna, Marković, Dejan, Čakić, Saša, Krstić, N., Marković, Danica, "The effects of hydroxyapatite and platelet rich plasma on apexogenesis in monkeys" in Acta veterinaria, 59, no. 2-3 (2009):277-289,
https://doi.org/10.2298/AVB0903277P . .

TY  - JOUR
AU  - Marković, Danica
AU  - Kojić, Zvezdana
AU  - Marinković, D.
AU  - Danilović, Vesna
AU  - Radovanović, Anita
AU  - Janaćković, Đorđe
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1473
AB  - The purpose of this study was to evaluate the tissue response induced by bioceramical materials (BCM) implanted in rat subcutaneous tissue. Thirty two rats were assigned to 6 groups, as following: control group i.e. not treated; dental ceramic (DC) implants; alfa tricalcium phosphate synthetised at 22 MPa (αTCP22) and at 45 MPa (αTCP45); hydroxyapatite synthetised at 22 MPa (HAP22) and at 45 MPa (HAP45). Contra lateral sides of all test animals represented the blank control surgically incisied at the paravertebral sockets. The experimental groups received subcutaneous implants of the test BCM materials. Samples were collected after 14 days and processed histologically. Inflammatory reactions were noted microscopically. The inflammatory infiltrate consisted of lymphocytes, polimorphonuclears, macrophages and mastocytes. At same time there were signs of increased fibrosis around the graft places. In conclusion, the implant sides compared with surgical incision wounds induced a foreign body-type granuloma with subsequent fibrosis around the grafts. The presence of a capsule at the interface with BCM in rat subcutis did not elicit any remarkable immune responses, thus being considered biocompatible. By evaluating different responses according to BCM, the most intense tissue reaction was noticed at HAP45, graded as severe, and mild to moderate for DC, HAP22 and alfa tricaltium phosphate.
AB  - Svrha ove studije je bila procena tkivnog odgovora indukovanog biokeramič kim materijalima (BCM) inplantiranim u subkutano tkivo pacova. Trideset dva pacova je bilo podeljeno u sledećih 6 grupa: kontrolna grupa-neoperisana; inplantirane sa: dentalnom keramikom (DC); alfa trikalcijum fosfatima sintetisanim na 22 MPa (αTCP22) i na 45 MPa (αTCP45); hidroksiapatitom sintetisanim na 22 MPa (HAP22) i na 45 MPa (HAP45). Kontralateralne strane svih životinja bile su slepa kontrola i urađene su kao džepovi hirurške incizije u subkutanom tkivu. Eksperimentalne grupe su imale subkutano inplantirane biokeramičke materijale. Uzorci su sakupljeni posle 14 dana i sprovedeni u histološkoj proceduri, a inflamatorna reakcija je praćena mikroskopski. Inflamatorni infiltrat se sastojao od limfocita, polimorfonuklearnih leukocita, makrofaga i mastocita. Istovremeno su uočeni znaci rastuće fibroze oko mesta graftova. Inplantirana mesta u poređenju sa hirurškim ranama indukuju granulom karakterističnog tipa na strano telo sa posledičnom fibrozom oko graftova, kapsulom na dodirnoj površini sa BCM u subkutisu pacova, ne izazivajući uočljivu imunološ ku reakciju, tako da odgovor tkiva ukazuje na biokompatibilnost. Procenjivanjem odgovora sa različitim BCM, zabeležena je najintenzivnija (snažna) tkivna reakcija kod HAP45 inplanta, a blaga do umerena kod DC, HAP22 i alfa trikalcijumfosfata.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Histological and immunohistochemical evaluations of rat soft tissue response to bioceramical implants
T1  - Histološka i imunohistohemijska procena reakcije mekog tkiva pacova na biokeramičke implante
VL  - 59
IS  - 2-3
SP  - 243
EP  - 253
DO  - 10.2298/AVB0903243M
ER  - 

@article{
author = "Marković, Danica and Kojić, Zvezdana and Marinković, D. and Danilović, Vesna and Radovanović, Anita and Janaćković, Đorđe",
year = "2009",
abstract = "The purpose of this study was to evaluate the tissue response induced by bioceramical materials (BCM) implanted in rat subcutaneous tissue. Thirty two rats were assigned to 6 groups, as following: control group i.e. not treated; dental ceramic (DC) implants; alfa tricalcium phosphate synthetised at 22 MPa (αTCP22) and at 45 MPa (αTCP45); hydroxyapatite synthetised at 22 MPa (HAP22) and at 45 MPa (HAP45). Contra lateral sides of all test animals represented the blank control surgically incisied at the paravertebral sockets. The experimental groups received subcutaneous implants of the test BCM materials. Samples were collected after 14 days and processed histologically. Inflammatory reactions were noted microscopically. The inflammatory infiltrate consisted of lymphocytes, polimorphonuclears, macrophages and mastocytes. At same time there were signs of increased fibrosis around the graft places. In conclusion, the implant sides compared with surgical incision wounds induced a foreign body-type granuloma with subsequent fibrosis around the grafts. The presence of a capsule at the interface with BCM in rat subcutis did not elicit any remarkable immune responses, thus being considered biocompatible. By evaluating different responses according to BCM, the most intense tissue reaction was noticed at HAP45, graded as severe, and mild to moderate for DC, HAP22 and alfa tricaltium phosphate., Svrha ove studije je bila procena tkivnog odgovora indukovanog biokeramič kim materijalima (BCM) inplantiranim u subkutano tkivo pacova. Trideset dva pacova je bilo podeljeno u sledećih 6 grupa: kontrolna grupa-neoperisana; inplantirane sa: dentalnom keramikom (DC); alfa trikalcijum fosfatima sintetisanim na 22 MPa (αTCP22) i na 45 MPa (αTCP45); hidroksiapatitom sintetisanim na 22 MPa (HAP22) i na 45 MPa (HAP45). Kontralateralne strane svih životinja bile su slepa kontrola i urađene su kao džepovi hirurške incizije u subkutanom tkivu. Eksperimentalne grupe su imale subkutano inplantirane biokeramičke materijale. Uzorci su sakupljeni posle 14 dana i sprovedeni u histološkoj proceduri, a inflamatorna reakcija je praćena mikroskopski. Inflamatorni infiltrat se sastojao od limfocita, polimorfonuklearnih leukocita, makrofaga i mastocita. Istovremeno su uočeni znaci rastuće fibroze oko mesta graftova. Inplantirana mesta u poređenju sa hirurškim ranama indukuju granulom karakterističnog tipa na strano telo sa posledičnom fibrozom oko graftova, kapsulom na dodirnoj površini sa BCM u subkutisu pacova, ne izazivajući uočljivu imunološ ku reakciju, tako da odgovor tkiva ukazuje na biokompatibilnost. Procenjivanjem odgovora sa različitim BCM, zabeležena je najintenzivnija (snažna) tkivna reakcija kod HAP45 inplanta, a blaga do umerena kod DC, HAP22 i alfa trikalcijumfosfata.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Histological and immunohistochemical evaluations of rat soft tissue response to bioceramical implants, Histološka i imunohistohemijska procena reakcije mekog tkiva pacova na biokeramičke implante",
volume = "59",
number = "2-3",
pages = "243-253",
doi = "10.2298/AVB0903243M"
}

Marković, D., Kojić, Z., Marinković, D., Danilović, V., Radovanović, A.,& Janaćković, Đ.. (2009). Histological and immunohistochemical evaluations of rat soft tissue response to bioceramical implants. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 243-253.
https://doi.org/10.2298/AVB0903243M

Marković D, Kojić Z, Marinković D, Danilović V, Radovanović A, Janaćković Đ. Histological and immunohistochemical evaluations of rat soft tissue response to bioceramical implants. in Acta veterinaria. 2009;59(2-3):243-253.
doi:10.2298/AVB0903243M .

Marković, Danica, Kojić, Zvezdana, Marinković, D., Danilović, Vesna, Radovanović, Anita, Janaćković, Đorđe, "Histological and immunohistochemical evaluations of rat soft tissue response to bioceramical implants" in Acta veterinaria, 59, no. 2-3 (2009):243-253,
https://doi.org/10.2298/AVB0903243M . .