TY  - JOUR
AU  - Paraš, S.
AU  - Trišić, Dijana
AU  - Ajtić, O.M.
AU  - Prokić, B.
AU  - Drobne, D.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2478
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
PB  - Mdpi Ag
T2  - Nanomaterials
T1  - Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats
VL  - 10
IS  - 5
DO  - 10.3390/nano10050918
ER  - 

@article{
author = "Paraš, S. and Trišić, Dijana and Ajtić, O.M. and Prokić, B. and Drobne, D. and Živković, Slavoljub and Jokanović, Vukoman",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
publisher = "Mdpi Ag",
journal = "Nanomaterials",
title = "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats",
volume = "10",
number = "5",
doi = "10.3390/nano10050918"
}

Paraš, S., Trišić, D., Ajtić, O.M., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V.. (2020). Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials
Mdpi Ag., 10(5).
https://doi.org/10.3390/nano10050918

Paraš S, Trišić D, Ajtić O, Prokić B, Drobne D, Živković S, Jokanović V. Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials. 2020;10(5).
doi:10.3390/nano10050918 .

Paraš, S., Trišić, Dijana, Ajtić, O.M., Prokić, B., Drobne, D., Živković, Slavoljub, Jokanović, Vukoman, "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats" in Nanomaterials, 10, no. 5 (2020),
https://doi.org/10.3390/nano10050918 . .

TY  - JOUR
AU  - Popović-Bajić, Marijana
AU  - Prokić, B.
AU  - Prokić, Bogomir
AU  - Jokanović, Vukoman
AU  - Danilović, Vesna
AU  - Živković, Slavoljub
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1789
AB  - The aim of this study was to examine the effect of Biodentine® and two new nanostructured materials based on active silicate cements on exposed tooth pulp of Vietnamese pigs. The study comprised 40 teeth in two Vietnamese pigs (24 months old). After class V cavity preparation, the pulp on each tooth was exposed using a small round bur. The following materials were applied on pulp exposures: Biodentine® (10 teeth), ALBO MPCA-I (10 teeth), and aLbO MPCA-II (10 teeth). In the control group, exposed pulp was covered with ProRoot MTA® (10 teeth). After the observation period of 28 days, the animals were sacrificed and the teeth prepared for histological analysis. Light microscope was used for the analysis of dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. In the group of Biodentine®, a complete dentin bridge was noted in 3 cases, while incomplete dentin bridge in the form of dental islets was detected in 4 cases. Nanostructured material ALBO-MPCA I provided complete dentin bridge formation in 5 teeth, in 3 teeth the formed dentin bridge was incomplete. ALBO MPCA-II showed complete closure of the pulp opening by dentin bridge in 4 samples, while in the same number of teeth it was incomplete. In the control group, 4 teeth showed a complete dentin bridge, whereas in 6 teeth it was incomplete. Histological analysis indicated favourable therapeutic effects of Biodentine® and the two materials ALBO-MPCA I and ALBO-MPCA II after teeth pulp capping in Vietnamese pigs. Pulp reaction was similar to that caused by ProRoot MTA®.
AB  - Cilj ovog rada je bio dase ispita efekat Biodentina i dva nova nanostrukturna materijala na bazi aktivnih cilikatnih cemenata na eksponiranu pulpu zuba vijentamskih svinja. Istraživanje je obuhvatilo 40 zuba i dve vijetnamske svinje starosti 24 meseca. Na svima zubima je urađena preparacije kaviteta V klase i malim okruglim svrdlom eksponirana komora pulpe. Na perforaciju pulpe aplikovani su materijali: Biodentin (10 zuba), ALBO-MPCA I (10 zuba), ALBO-MPCA II (10 zuba). U kontrolnoj grupi pulpa je prekrivena MTA-om (10 zuba). Nakon observacionog perioda od 28 dana, životinje su žrtvovane i pripremljene za histološku analizu. Svetlosnom mikroskopijom analizirano je formiranje dentinskog mostića, postojanje reorganizacije tkiva, prisustvo inflamacije i bakterija u pulpi. Kod Biodentina kompletan dentinski mostić je zabeležen u 3 slučaja, a inkompletan dentinski mostić u vidu dentinskih ostrvaca u 4 slučaja. Nanostrukturni materijal ALBO-MPCA I je doveo do stvaranja kompletnog dentinskog mostića kod 5 zuba, a kod 3 zuba dentinski mostić je bio inkompletan. ALBO-MPCA II je pokazao kompletno zatvaranje komore pulpe dentinskim mostićem u 4 uzorka, dok je kod 4 uzorka on bio inkompletan. Kontrolni materijal MTA je kod 4 zuba imao stvoren kompletan dentinski mosti}, a u kod 6 zuba je on bio inkompletan. Histološka analiza je ukazala na povoljne terapijske efekte i Biodentina i dva novosintetisana materijala ALBO-MPCA I i ALBO-MPCA II u direktnom prekrivanju pulpezuba vijentamskih svinja. Reakcija pulpe bilaje slična onima koje je izazvao MTA.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine® on pulp tissue
T1  - Histološka procena stanja zubne pulpe nakon direktnog prekrivanja novim nanostrukturnim materijalima na bazi aktivnih silikatnih cemenata i biodentina
VL  - 63
IS  - 2-3
SP  - 347
EP  - 360
DO  - 10.2298/AVB1303347P
ER  - 

@article{
author = "Popović-Bajić, Marijana and Prokić, B. and Prokić, Bogomir and Jokanović, Vukoman and Danilović, Vesna and Živković, Slavoljub",
year = "2013",
abstract = "The aim of this study was to examine the effect of Biodentine® and two new nanostructured materials based on active silicate cements on exposed tooth pulp of Vietnamese pigs. The study comprised 40 teeth in two Vietnamese pigs (24 months old). After class V cavity preparation, the pulp on each tooth was exposed using a small round bur. The following materials were applied on pulp exposures: Biodentine® (10 teeth), ALBO MPCA-I (10 teeth), and aLbO MPCA-II (10 teeth). In the control group, exposed pulp was covered with ProRoot MTA® (10 teeth). After the observation period of 28 days, the animals were sacrificed and the teeth prepared for histological analysis. Light microscope was used for the analysis of dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. In the group of Biodentine®, a complete dentin bridge was noted in 3 cases, while incomplete dentin bridge in the form of dental islets was detected in 4 cases. Nanostructured material ALBO-MPCA I provided complete dentin bridge formation in 5 teeth, in 3 teeth the formed dentin bridge was incomplete. ALBO MPCA-II showed complete closure of the pulp opening by dentin bridge in 4 samples, while in the same number of teeth it was incomplete. In the control group, 4 teeth showed a complete dentin bridge, whereas in 6 teeth it was incomplete. Histological analysis indicated favourable therapeutic effects of Biodentine® and the two materials ALBO-MPCA I and ALBO-MPCA II after teeth pulp capping in Vietnamese pigs. Pulp reaction was similar to that caused by ProRoot MTA®., Cilj ovog rada je bio dase ispita efekat Biodentina i dva nova nanostrukturna materijala na bazi aktivnih cilikatnih cemenata na eksponiranu pulpu zuba vijentamskih svinja. Istraživanje je obuhvatilo 40 zuba i dve vijetnamske svinje starosti 24 meseca. Na svima zubima je urađena preparacije kaviteta V klase i malim okruglim svrdlom eksponirana komora pulpe. Na perforaciju pulpe aplikovani su materijali: Biodentin (10 zuba), ALBO-MPCA I (10 zuba), ALBO-MPCA II (10 zuba). U kontrolnoj grupi pulpa je prekrivena MTA-om (10 zuba). Nakon observacionog perioda od 28 dana, životinje su žrtvovane i pripremljene za histološku analizu. Svetlosnom mikroskopijom analizirano je formiranje dentinskog mostića, postojanje reorganizacije tkiva, prisustvo inflamacije i bakterija u pulpi. Kod Biodentina kompletan dentinski mostić je zabeležen u 3 slučaja, a inkompletan dentinski mostić u vidu dentinskih ostrvaca u 4 slučaja. Nanostrukturni materijal ALBO-MPCA I je doveo do stvaranja kompletnog dentinskog mostića kod 5 zuba, a kod 3 zuba dentinski mostić je bio inkompletan. ALBO-MPCA II je pokazao kompletno zatvaranje komore pulpe dentinskim mostićem u 4 uzorka, dok je kod 4 uzorka on bio inkompletan. Kontrolni materijal MTA je kod 4 zuba imao stvoren kompletan dentinski mosti}, a u kod 6 zuba je on bio inkompletan. Histološka analiza je ukazala na povoljne terapijske efekte i Biodentina i dva novosintetisana materijala ALBO-MPCA I i ALBO-MPCA II u direktnom prekrivanju pulpezuba vijentamskih svinja. Reakcija pulpe bilaje slična onima koje je izazvao MTA.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine® on pulp tissue, Histološka procena stanja zubne pulpe nakon direktnog prekrivanja novim nanostrukturnim materijalima na bazi aktivnih silikatnih cemenata i biodentina",
volume = "63",
number = "2-3",
pages = "347-360",
doi = "10.2298/AVB1303347P"
}

Popović-Bajić, M., Prokić, B., Prokić, B., Jokanović, V., Danilović, V.,& Živković, S.. (2013). Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine® on pulp tissue. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(2-3), 347-360.
https://doi.org/10.2298/AVB1303347P

Popović-Bajić M, Prokić B, Prokić B, Jokanović V, Danilović V, Živković S. Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine® on pulp tissue. in Acta veterinaria. 2013;63(2-3):347-360.
doi:10.2298/AVB1303347P .

Popović-Bajić, Marijana, Prokić, B., Prokić, Bogomir, Jokanović, Vukoman, Danilović, Vesna, Živković, Slavoljub, "Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine® on pulp tissue" in Acta veterinaria, 63, no. 2-3 (2013):347-360,
https://doi.org/10.2298/AVB1303347P . .