Jokanović, Vukoman

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orcid::0000-0002-2976-8238
  • Jokanović, Vukoman (61)
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Author's Bibliography

Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats

Paraš, S.; Trišić, Dijana; Ajtić, O.M.; Prokić, B.; Drobne, D.; Živković, Slavoljub; Jokanović, Vukoman

(Mdpi Ag, 2020)

TY  - JOUR
AU  - Paraš, S.
AU  - Trišić, Dijana
AU  - Ajtić, O.M.
AU  - Prokić, B.
AU  - Drobne, D.
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2478
AB  - Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.
PB  - Mdpi Ag
T2  - Nanomaterials
T1  - Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats
VL  - 10
IS  - 5
DO  - 10.3390/nano10050918
ER  - 
@article{
author = "Paraš, S. and Trišić, Dijana and Ajtić, O.M. and Prokić, B. and Drobne, D. and Živković, Slavoljub and Jokanović, Vukoman",
year = "2020",
abstract = "Novel three-dimensional (3D) nanohydroxyapatite-PLGA scaffolds with high porosity was developed to better mimic mineral component and microstructure of natural bone. To perform a final assessment of this nanomaterial as a potential bone substitute, its toxicological profile was particularly investigated. Therefore, we performed a comet assay on human monocytes for in vitro genotoxicity investigation, and the systemic subchronic toxicity investigation on rats being per oral feed with exactly administrated extract quantities of the nano calcium hydroxyapatite covered with tiny layers of PLGA (ALBO-OS) for 120 days. Histological and stereological parameters of the liver, kidney, and spleen tissue were analyzed. Comet assay revealed low genotoxic potential, while histological analysis and stereological investigation revealed no significant changes in exposed animals when compared to controls, although the volume density of blood sinusoids and connective tissue, as well as numerical density and number of mitosis were slightly increased. Additionally, despite the significantly increased average number of the Ki67 and slightly increased number of CD68 positive cells in the presence of ALBO-OS, immunoreactive cells proliferation was almost neglected. Blood analyses showed that all of the blood parameters in rats fed with extract nanomaterial are comparable with corresponding parameters of no feed rats, taken as blind probe. This study contributes to the toxicological profiling of ALBO-OS scaffold for potential future application in bone tissue engineering.",
publisher = "Mdpi Ag",
journal = "Nanomaterials",
title = "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats",
volume = "10",
number = "5",
doi = "10.3390/nano10050918"
}
Paraš, S., Trišić, D., Ajtić, O.M., Prokić, B., Drobne, D., Živković, S.,& Jokanović, V.. (2020). Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials
Mdpi Ag., 10(5).
https://doi.org/10.3390/nano10050918
Paraš S, Trišić D, Ajtić O, Prokić B, Drobne D, Živković S, Jokanović V. Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats. in Nanomaterials. 2020;10(5).
doi:10.3390/nano10050918 .
Paraš, S., Trišić, Dijana, Ajtić, O.M., Prokić, B., Drobne, D., Živković, Slavoljub, Jokanović, Vukoman, "Toxicological profile of nanostructured bone substitute based on hydroxyapatite and poly(Lactide-co-glycolide) after subchronic oral exposure of rats" in Nanomaterials, 10, no. 5 (2020),
https://doi.org/10.3390/nano10050918 . .
3
3
3

Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study

Ćetenović, Bojana; Marković, Dejan; Gatman, Džejms; Perić, Tamara; Jokanović, Vukoman

(Srpsko lekarsko društvo, Beograd, 2019)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Marković, Dejan
AU  - Gatman, Džejms
AU  - Perić, Tamara
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2438
AB  - Introduction/Objective the purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results the positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p  lt  0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion the MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice.
AB  - Uvod/Cilj Svrha ove studije bila je procena efikasnosti endodontskog tretmana traumatizovanih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama posle definitivne opturacije mineralnim trioksidnim agregatom (MTA). Softver za analizu slike Adobe Photoshop CS korišćen je za procenu regeneracije. Metode Dvadeset i sedam traumatizovanih jednokorenih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama tretirani su nehirurškim procedurama pomoću triantibiotske paste i kalcijum-hidroksida kao intrakanalnog medikamenta. Definitivna opturacija izvedena je pomoću ProRoot MTA ili MTA+ Cerkamed. Kontrolni pregledi obavljeni su tri, šest, 12 i 24 meseca posle završetka lečenja. Rezultati Pozitivan klinički ishod zabeležen je u 24 (88,9%) slučaja, dok je radiografski uspeh bio prisutan u 26 (96,3%) slučaja. Statistički značajno smanjenje veličine periapikalnih lezija primećeno je u svim periodima (p  lt  0,001). Nije bilo statistički značajne razlike između dva testirana MTA materijala (p > 0,05). Zaključak MTA proizvodi su efikasni u opturaciji korenskih kanala i čini se da doprinose značajnom smanjenju ili potpunoj regresiji periapikalnih lezija u tretiranim zubima. Prikazana metodologija može biti predložena za svakodnevnu kliničku praksu.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study
T1  - Endodontsko lečenje traumatizovanih zuba sa hroničnim periapikalnim lezijama primenom antibiotske paste i opturacije mineralnim trioksidnim agregatom - preliminarna studija
VL  - 147
IS  - 5-6
SP  - 270
EP  - 275
DO  - 10.2298/SARH180301018C
ER  - 
@article{
author = "Ćetenović, Bojana and Marković, Dejan and Gatman, Džejms and Perić, Tamara and Jokanović, Vukoman",
year = "2019",
abstract = "Introduction/Objective the purpose of this study was to assess effectiveness of endodontic root canal procedures in traumatized permanent teeth with necrotic pulps and chronic periapical lesions after definitive obturation with mineral trioxide aggregate (MTA) products. Adobe Photoshop CS (San Jose, CA, USA) image-analysis software was used for healing assessment. Methods Twenty-seven traumatized single-rooted permanent teeth diagnosed with necrotic pulps and chronic periapical lesions were treated with non-surgical procedure using a tri-antibiotic paste and calcium hydroxide as intra-canal medication. Definitive obturation was performed with ProRoot MTA (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA) or MTA+ Cerkamed (Cerkamed, Stalowa Wola, Poland). Control follow-ups were done three, six, 12, and 24 months following the completion of treatment. Results the positive clinical outcome was recorded in 24 (88.9%) cases, while radiographic success was present in 26 (96.3%) cases. A statistically significant decrease in the sizes of periapical lesions was consistently observed at follow-up periods (p  lt  0.001). There was no statistically significant difference between the two tested MTA materials (p > 0.05). Conclusion the MTA products were effective for the root canal obturation and appeared to contribute to the significant reduction or complete regression of periapical lesions in teeth treated. The presented procedure may be proposed for everyday clinical practice., Uvod/Cilj Svrha ove studije bila je procena efikasnosti endodontskog tretmana traumatizovanih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama posle definitivne opturacije mineralnim trioksidnim agregatom (MTA). Softver za analizu slike Adobe Photoshop CS korišćen je za procenu regeneracije. Metode Dvadeset i sedam traumatizovanih jednokorenih stalnih zuba sa nekrotičnom pulpom i hroničnim periapikalnim lezijama tretirani su nehirurškim procedurama pomoću triantibiotske paste i kalcijum-hidroksida kao intrakanalnog medikamenta. Definitivna opturacija izvedena je pomoću ProRoot MTA ili MTA+ Cerkamed. Kontrolni pregledi obavljeni su tri, šest, 12 i 24 meseca posle završetka lečenja. Rezultati Pozitivan klinički ishod zabeležen je u 24 (88,9%) slučaja, dok je radiografski uspeh bio prisutan u 26 (96,3%) slučaja. Statistički značajno smanjenje veličine periapikalnih lezija primećeno je u svim periodima (p  lt  0,001). Nije bilo statistički značajne razlike između dva testirana MTA materijala (p > 0,05). Zaključak MTA proizvodi su efikasni u opturaciji korenskih kanala i čini se da doprinose značajnom smanjenju ili potpunoj regresiji periapikalnih lezija u tretiranim zubima. Prikazana metodologija može biti predložena za svakodnevnu kliničku praksu.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study, Endodontsko lečenje traumatizovanih zuba sa hroničnim periapikalnim lezijama primenom antibiotske paste i opturacije mineralnim trioksidnim agregatom - preliminarna studija",
volume = "147",
number = "5-6",
pages = "270-275",
doi = "10.2298/SARH180301018C"
}
Ćetenović, B., Marković, D., Gatman, D., Perić, T.,& Jokanović, V.. (2019). Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 147(5-6), 270-275.
https://doi.org/10.2298/SARH180301018C
Ćetenović B, Marković D, Gatman D, Perić T, Jokanović V. Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study. in Srpski arhiv za celokupno lekarstvo. 2019;147(5-6):270-275.
doi:10.2298/SARH180301018C .
Ćetenović, Bojana, Marković, Dejan, Gatman, Džejms, Perić, Tamara, Jokanović, Vukoman, "Endodontic treatment of traumatized teeth with chronic periapical lesions using antibiotic paste and mineral trioxide aggregate obturation: A preliminary study" in Srpski arhiv za celokupno lekarstvo, 147, no. 5-6 (2019):270-275,
https://doi.org/10.2298/SARH180301018C . .
4
3
3

Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition

Magić, Marko; Čolović, Božana; Jokanović, Vukoman; Vasilijić, Saša; Marković, Milan; Vučević, Dragana; Rudolf, Rebeka; Čolić, Snježana; Čolić, Miodrag

(Vojnomedicinska akademija - Institut za naučne informacije, Beograd, 2019)

TY  - JOUR
AU  - Magić, Marko
AU  - Čolović, Božana
AU  - Jokanović, Vukoman
AU  - Vasilijić, Saša
AU  - Marković, Milan
AU  - Vučević, Dragana
AU  - Rudolf, Rebeka
AU  - Čolić, Snježana
AU  - Čolić, Miodrag
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2445
AB  - Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo.
AB  - Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition
T1  - Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme
VL  - 76
IS  - 5
SP  - 492
EP  - 501
DO  - 10.2298/VSP170809097M
ER  - 
@article{
author = "Magić, Marko and Čolović, Božana and Jokanović, Vukoman and Vasilijić, Saša and Marković, Milan and Vučević, Dragana and Rudolf, Rebeka and Čolić, Snježana and Čolić, Miodrag",
year = "2019",
abstract = "Background/Aim. The deposition of hydroxyapatite (HAP) on the surface of titanium (Ti) alloys enhances bioactivity and osseointegration of the alloys which are widely used as implant materials in dentistry and orthopaedic surgery. However, the stability of HAP and subsequent biocompatibility of such alloys depends on the coating technique. The aim of this work was to test the cytotoxicity of a Ti alloy (Ti6Al4V), coated with HAP by a new plasma deposition method. Methods. The Ti6Al4V samples prepared as discs, 10 mm in diameter and 2 mm in thickness, were coated with HAP (one or both sides of the alloy) by an innovative atmospheric plasma jet method. The cytotoxicity of uncoated and HAP coated Ti6Al4V samples was evaluated by examining the morphological changes and viability of L929 fibroblasts in direct contact with the test materials. Adequate negative (polystyrene) and positive (nickel) control discs of the same size were used. The indirect cytotoxicity was determined by cultivating L929 cells with conditioning medium (CM), prepared as extract of the test samples incubated in the complete Roswell Park Memorial Institute (RPMI) 1640 medium for cell cultures. The cytotoxic effect was evaluated based on the degree of metabolic activity, necrosis, apoptosis and proliferation of L929 cells, using the appropriate assays. Results. Uncoated and one side HAP coated Ti6Al4V alloys were classified as non-cytotoxic according to the current ISO 10993-5 criteria, whereas two sides HAP coated Ti6Al4V alloy samples were slightly-moderate cytotoxic. The cytotoxicity manifested as the inhibition of metabolic activity and proliferation of L929 cells as well as the induction of their apoptosis and necrosis was significantly reduced by conditioning of HAP/Ti6Al4V alloys for 24 hours. The cytotoxic effect of HAP/Ti6Al4V CM only partly decreased in the presence of nifelate, a calcium (Ca) channel blocker, suggesting that Ca ions were not the only responsible cytotoxic agent. Conclusion. The original HAP coating procedure by atmospheric plasma spraying with high energy input enables the production of the stable adhesive coatings on Ti6Al4V alloys. Their cytotoxicity, which depends on the quantity of HAP coating layer, could be significantly reduced up to the non-cytotoxic level by prior conditioning of the alloys in culture medium. Such a procedure, which removes leachable toxic components, could be useful before implantation of HAP coated alloys in vivo., Uvod/Cilj. Oblaganje površine legura titana (Ti) hidroksiapatitom (HAP) poboljšava bioaktivnost i oseointegraciju Ti legura, koje se široko koriste kao implantacioni materijali u stomatologiji i ortopediji. Međutim, stabilnost HAP prevlake i biokompatibilnost takvih legura zavise od primenjene tehnike oblaganja. Cilj ovog rada je bio da se ispita citotoksičnost Ti6Al4V legure obložene sa HAP pomoću plazme korišćenjem originalne metode. Metode. Uzorci Ti6Al4V legure u obliku diska, prečnika 10 mm, debljine 2 mm su presvučeni sa HAP (jednostrano ili obostrano) mlazom atmosferske plazme. Citotoksičnost neobložene i HAP-om obloženih Ti6Al4V legura je ispitivana na osnovu morfoloških karakteristika i vijabilnosti L929 fibroblasta u direktnom kontaktu ćelija sa test materijalima. Odgovarajuća negativna kontrola (polistirenski diskovi) i pozitivna kontrola (diskovi od nikla) istih veličina kao i diskovi Ti6Al4V legura su takođe uključeni u eksperimente. Indirektna citotoksičnost je procenjivana nakon kultivisanja L929 ćelija sa kondicioniranim medijumom (CM), koji je predstavljao ekstrakt testiranih uzoraka inkubiranih u kompletnom Roswel Park Memorial Institute (RPMI) 1640 medijumu za ćelijske kulture. Citotoksični efekat CM je procenjivan na osnovu stepena metaboličke aktivnosti, nekroze, apoptoze i proliferacije L929 ćelija, korišćenjem adekvatnih testova. Rezultati. Neobložena Ti6Al4V legura i Ti6Al4V legura obložena jednostrano sa HAP su okarakterisane kao necitotoksične na osnovu ISO 10993-5 kriterijuma, dok je Ti6Al4V legura obložena sa HAP obostrano pokazivala blagu do umerenu citotoksičnost. Citotoksičnost, koja se manifestovala smanjenjem metaboličke aktivnosti i proliferacije L929 ćelija kao i indukcijom njihove apoptoze i nekroze, je bila značajno smanjena ako su uzorci HAP-om presvučenih legura kondicionirani u medijumu u toku 24 časa. Citotoksičnost CM pripremljenih od Ti6Al4V legura obloženih sa HAP je bila samo delimično smanjena u prisustvu nifelata, blokatora kalcijumovih (Ca) kanala, što ukazuje da Ca joni nisu jedini citotoksični faktor. Zaključak. Originalna metoda oblaganja Ti6Al4V legure sa HAP pomoću atmosferske plazme u obliku spreja visoke energije omogućava stabilnu adheziju prevlake. Citotoksičnost ovako obrađene legure, koja zavisi od količine nanetog HAP, se može znatno smanjiti do necitotoksičnog nivoa prethodnim kondicioniranjem u medijumu. Ova procedura, kojom se uklanjaju rastvorljive toksične komponente, može biti korisna pre in vivo implantacije legura obloženih sa HAP.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition, Citotoksičnost legure titana obložene hidroksiapatitom pomoću mlaza plazme",
volume = "76",
number = "5",
pages = "492-501",
doi = "10.2298/VSP170809097M"
}
Magić, M., Čolović, B., Jokanović, V., Vasilijić, S., Marković, M., Vučević, D., Rudolf, R., Čolić, S.,& Čolić, M.. (2019). Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(5), 492-501.
https://doi.org/10.2298/VSP170809097M
Magić M, Čolović B, Jokanović V, Vasilijić S, Marković M, Vučević D, Rudolf R, Čolić S, Čolić M. Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition. in Vojnosanitetski pregled. 2019;76(5):492-501.
doi:10.2298/VSP170809097M .
Magić, Marko, Čolović, Božana, Jokanović, Vukoman, Vasilijić, Saša, Marković, Milan, Vučević, Dragana, Rudolf, Rebeka, Čolić, Snježana, Čolić, Miodrag, "Cytotoxicity of a titanium alloy coated with hydroxyapatite by plasma jet deposition" in Vojnosanitetski pregled, 76, no. 5 (2019):492-501,
https://doi.org/10.2298/VSP170809097M . .

A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis

Čolović, Božana; Janković, Ognjenka; Živković, Slavoljub; Zizak, Zeljko; Zizak, Irina Besu; Jokanović, Vukoman

(Elsevier Sci Ltd, Oxford, 2019)

TY  - JOUR
AU  - Čolović, Božana
AU  - Janković, Ognjenka
AU  - Živković, Slavoljub
AU  - Zizak, Zeljko
AU  - Zizak, Irina Besu
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2442
AB  - The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.
PB  - Elsevier Sci Ltd, Oxford
T2  - Ceramics International
T1  - A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
VL  - 45
IS  - 7
SP  - 9211
EP  - 9218
DO  - 10.1016/j.ceramint.2019.01.266
ER  - 
@article{
author = "Čolović, Božana and Janković, Ognjenka and Živković, Slavoljub and Zizak, Zeljko and Zizak, Irina Besu and Jokanović, Vukoman",
year = "2019",
abstract = "The synthesis of a new endodontic mixture based on calcium aluminate cement is reported. Physico-chemical and mechanical properties of the mixture were investigated before and after various hydration periods. Phase analysis of the mixture was performed using X-ray diffraction (XRD), while morphological analysis was performed by scanning electron microscopy (SEM) equipped with EDS which revealed elemental composition of the mixture sample. Investigations of mechanical properties showed that this new endodontic mixture possessed good mechanical properties (compressive strength after 28 days aging was 94 MPa) and relatively short setting time (2 h). Additionally, cytotoxicity of the mixture was investigated by MTT assay, while genotoxicity was investigated by Comet assay, using MRC-5 cell line. The obtained results make this material very promising for potential application in dental practice.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Ceramics International",
title = "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis",
volume = "45",
number = "7",
pages = "9211-9218",
doi = "10.1016/j.ceramint.2019.01.266"
}
Čolović, B., Janković, O., Živković, S., Zizak, Z., Zizak, I. B.,& Jokanović, V.. (2019). A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International
Elsevier Sci Ltd, Oxford., 45(7), 9211-9218.
https://doi.org/10.1016/j.ceramint.2019.01.266
Čolović B, Janković O, Živković S, Zizak Z, Zizak IB, Jokanović V. A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis. in Ceramics International. 2019;45(7):9211-9218.
doi:10.1016/j.ceramint.2019.01.266 .
Čolović, Božana, Janković, Ognjenka, Živković, Slavoljub, Zizak, Zeljko, Zizak, Irina Besu, Jokanović, Vukoman, "A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis" in Ceramics International, 45, no. 7 (2019):9211-9218,
https://doi.org/10.1016/j.ceramint.2019.01.266 . .
3
2
3

Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate

Radović, Igor; Stojanović, Nikola; Krunić, Jelena; Davidović, Lado; Lečić, Jelena; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2019)

TY  - JOUR
AU  - Radović, Igor
AU  - Stojanović, Nikola
AU  - Krunić, Jelena
AU  - Davidović, Lado
AU  - Lečić, Jelena
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2430
AB  - Introduction Materials used for root canal obturation and root perforation are expected to have, in addition to preventing apical, lateral and coronary leakage, antimicrobial effects on microorganisms that are not accessible to chemomechanical instrumentation and intra-canal medications. The aim of this study was to evaluate the antimicrobial effect of a novel calcium aluminate-based endodontic nanostructured biomaterial (ALBO-MCCA) using agar diffusion test. Material and methods the two materials were tested in the study. The nanostructured calcium aluminate was synthesized by the hydrothermal sol-gel method from individual components of calcium aluminate (CaOxAl2 O3 ), calcite (CaCO3 ) and barium sulfate (BaSO4 ) as radiocontrast agent in the ratio of 2:2:1 according to V. Jokanovic's recipe. The other used material was calcium silicate MTA Angelus (Londrina, Brazil). The antimicrobial effect was assessed using agar diffusion test. Standard strains of Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, cultured on blood Mueller-Hinton agar and Candida albicans cultivated on Sabouraud Dextrose Agar, were used as test microorganisms. Results the best antimicrobial effect after 24 h both materials showed against S. aureus. The mean values of the growth inhibition zone for ALBO MCCA were 5.7 ± 0.25 mm and MTA 6.2 ± 0.4 mm. The ALBO MCCA material showed slightly stronger antibacterial effect against E. coli compared to MTA (p  lt  0.05), whereas none of the materials showed antibacterial effect against E. faecalis. Conclusion the ALBO MCCA material showed certain antibacterial effect on Escherichia coli, Staphylococcus aureus and Candida albicans that was comparable to antibacterial effect of MTA.
AB  - Uvod Od materijala koji se koriste za opturaciju kanala korena i perforacija na korenu se očekuje da pored sprečavanja apikalnog, lateralnog i koronarnog curenja, poseduju i antimikrobno delovanje na mikroorganizme, koji nisu dostupni hemomehaničkoj obradi i intrakanalnim medikamentima. Cilj ovog istraživanja je bio da se testom difuzije u agaru proceni antimikrobni efekat novog endodontskog nanostrukturnog biomaterijala na bazi kalcijum-aluminata (ALBO-MCCA). Materijal i metode U istraživanju su korišćena dva materijala. Nanostrukturni kalcijum-aluminat je sintetisan hidrotermalnom sol-gel metodom od pojedinačnih komponenata kalcijum-aluminata (CaO·Al2 O3 ), kalcita (CaCO3 ) i barijum-sulfata (BaSO4 ) kao rendgen-kontrastnog sredstva u odnosu 2 : 2 : 1 prema recepturi V. Jokanovića. Drugi korišćeni materijal je kalcijum-silikatni MTA Angelus (Londrina, Brazil). Antimikrobni efekat je ispitivan testom difuzije u agaru. Kao test mikroorganizmi korišćeni su standardni sojevi Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, koji su kultivisani na krvnom agaru MuellerHinton, dok je C. albicans kultivisana na agaru Sabouraud Dextrose. Rezultati Najbolji antimikrobni efekat nakon 24 h oba materijala su pokazala prema bakteriji S. aureus. Izmerene prosečne vrednosti zone inhibicije rasta iznosile su kod ALBO-MCCA (5,7 ± 0,25 mm), odnosno kod MTA (6,2 ± 0,4 mm). Materijal ALBO-MCCA je pokazao nešto veći antibakterijski efekat prema bakteriji E. coli u odnosu na MTA (p  lt  0,05), dok prema bakteriji E. faecalis nijedan materijal nije ispoljio antibakterijski efekat. Zaključak Materijal ALBO-MCCA je ispoljio određeni antibakterijski efekat na bakterije Escherichia coli, Staphylococcus aureus i Candida albicans komparabilan sa antibakterijskim efektom MTA.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate
T1  - Antimikrobna aktivnost novosintetisanog endodontskog nanomaterijala na bazi kalcijum-aluminata
VL  - 66
IS  - 3
SP  - 113
EP  - 119
DO  - 10.2478/sdj-2019-0012
ER  - 
@article{
author = "Radović, Igor and Stojanović, Nikola and Krunić, Jelena and Davidović, Lado and Lečić, Jelena and Jokanović, Vukoman and Živković, Slavoljub",
year = "2019",
abstract = "Introduction Materials used for root canal obturation and root perforation are expected to have, in addition to preventing apical, lateral and coronary leakage, antimicrobial effects on microorganisms that are not accessible to chemomechanical instrumentation and intra-canal medications. The aim of this study was to evaluate the antimicrobial effect of a novel calcium aluminate-based endodontic nanostructured biomaterial (ALBO-MCCA) using agar diffusion test. Material and methods the two materials were tested in the study. The nanostructured calcium aluminate was synthesized by the hydrothermal sol-gel method from individual components of calcium aluminate (CaOxAl2 O3 ), calcite (CaCO3 ) and barium sulfate (BaSO4 ) as radiocontrast agent in the ratio of 2:2:1 according to V. Jokanovic's recipe. The other used material was calcium silicate MTA Angelus (Londrina, Brazil). The antimicrobial effect was assessed using agar diffusion test. Standard strains of Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, cultured on blood Mueller-Hinton agar and Candida albicans cultivated on Sabouraud Dextrose Agar, were used as test microorganisms. Results the best antimicrobial effect after 24 h both materials showed against S. aureus. The mean values of the growth inhibition zone for ALBO MCCA were 5.7 ± 0.25 mm and MTA 6.2 ± 0.4 mm. The ALBO MCCA material showed slightly stronger antibacterial effect against E. coli compared to MTA (p  lt  0.05), whereas none of the materials showed antibacterial effect against E. faecalis. Conclusion the ALBO MCCA material showed certain antibacterial effect on Escherichia coli, Staphylococcus aureus and Candida albicans that was comparable to antibacterial effect of MTA., Uvod Od materijala koji se koriste za opturaciju kanala korena i perforacija na korenu se očekuje da pored sprečavanja apikalnog, lateralnog i koronarnog curenja, poseduju i antimikrobno delovanje na mikroorganizme, koji nisu dostupni hemomehaničkoj obradi i intrakanalnim medikamentima. Cilj ovog istraživanja je bio da se testom difuzije u agaru proceni antimikrobni efekat novog endodontskog nanostrukturnog biomaterijala na bazi kalcijum-aluminata (ALBO-MCCA). Materijal i metode U istraživanju su korišćena dva materijala. Nanostrukturni kalcijum-aluminat je sintetisan hidrotermalnom sol-gel metodom od pojedinačnih komponenata kalcijum-aluminata (CaO·Al2 O3 ), kalcita (CaCO3 ) i barijum-sulfata (BaSO4 ) kao rendgen-kontrastnog sredstva u odnosu 2 : 2 : 1 prema recepturi V. Jokanovića. Drugi korišćeni materijal je kalcijum-silikatni MTA Angelus (Londrina, Brazil). Antimikrobni efekat je ispitivan testom difuzije u agaru. Kao test mikroorganizmi korišćeni su standardni sojevi Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, koji su kultivisani na krvnom agaru MuellerHinton, dok je C. albicans kultivisana na agaru Sabouraud Dextrose. Rezultati Najbolji antimikrobni efekat nakon 24 h oba materijala su pokazala prema bakteriji S. aureus. Izmerene prosečne vrednosti zone inhibicije rasta iznosile su kod ALBO-MCCA (5,7 ± 0,25 mm), odnosno kod MTA (6,2 ± 0,4 mm). Materijal ALBO-MCCA je pokazao nešto veći antibakterijski efekat prema bakteriji E. coli u odnosu na MTA (p  lt  0,05), dok prema bakteriji E. faecalis nijedan materijal nije ispoljio antibakterijski efekat. Zaključak Materijal ALBO-MCCA je ispoljio određeni antibakterijski efekat na bakterije Escherichia coli, Staphylococcus aureus i Candida albicans komparabilan sa antibakterijskim efektom MTA.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate, Antimikrobna aktivnost novosintetisanog endodontskog nanomaterijala na bazi kalcijum-aluminata",
volume = "66",
number = "3",
pages = "113-119",
doi = "10.2478/sdj-2019-0012"
}
Radović, I., Stojanović, N., Krunić, J., Davidović, L., Lečić, J., Jokanović, V.,& Živković, S.. (2019). Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 66(3), 113-119.
https://doi.org/10.2478/sdj-2019-0012
Radović I, Stojanović N, Krunić J, Davidović L, Lečić J, Jokanović V, Živković S. Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate. in Stomatološki glasnik Srbije. 2019;66(3):113-119.
doi:10.2478/sdj-2019-0012 .
Radović, Igor, Stojanović, Nikola, Krunić, Jelena, Davidović, Lado, Lečić, Jelena, Jokanović, Vukoman, Živković, Slavoljub, "Antibacterial activity of newly synthesized endodontic nanomaterial based on calcium aluminate" in Stomatološki glasnik Srbije, 66, no. 3 (2019):113-119,
https://doi.org/10.2478/sdj-2019-0012 . .

Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics

Jokanović, Vukoman; Čolović, Božana; Ćetenović, Bojana; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2019)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Ćetenović, Bojana
AU  - Živković, Slavoljub
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2427
AB  - Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties.
AB  - U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics
T1  - Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji
VL  - 66
IS  - 2
SP  - 87
EP  - 96
DO  - 10.2478/sdj-2019-0010
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Ćetenović, Bojana and Živković, Slavoljub",
year = "2019",
abstract = "Various magnetron-sputtering methods for surface modification of shape memory alloys (SMA) are described in this paper. These methods belong to the most effective methods, which enable mechanical reinforcing of the SMA, showing numerous advantages over conventional methods of electro-polishing. In addition, surface modified SMA, particularly with equiatomic TiNi coatings, is crucial for further development of various endodontic instruments; wires and brackets used for orthodontic teeth movements. Active coatings with bactericide properties and coatings that can be used as barrier for release of toxic Ni ions from the bulk body of SMA obtained by various magnetron-sputtering methods can be successfully combined. Therefore, the review of these methods is given in this paper, with their main characteristics and drawbacks. Magnetron sputtering deposition involves surface modification of SMA in a single-layer, multilayer, graded layers, and nanocomposite thin coatings for obtaining systems with superior "functional" characteristics. These are hardness, scratch, abrasion, and erosion resistance, improved adhesion to various technologically important substrate materials such as polymers, hydrophobicity or hydrophilicity, long-term chemical, thermal, and environmental stability, gas and vapor impermeability, and others. This paper is critical review of the advances in the development of magnetron sputtering modified SMA products in dentistry, with in advance predictable physicochemical, structural and antimicrobial properties., U ovom radu su opisane različite metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura (shape memory alloys - SMA). Ove metode spadaju u najefikasnije metode koje omogućavaju mehaničko ojačavanje SMA, pokazujući brojne prednosti u odnosu na konvencionalne metode elektropoliranja, koje su najčešće korišćene u savremenoj ortodontskoj i endodontskoj praksi. Pored toga, površinski modifikovane SMA, posebno sa ekviatomskim Ti i Ni udelima unutar prevlake, presudne su za dalji razvoj različitih endodontskih instrumenata, žica i konzola koje se koriste za ortodontska pomeranja zuba. Aktivne prevlake sa baktericidnim svojstvima i prevlake koje služe kao barijere protiv otpuštanja toksičnih Ni jona iz SMA unutar organizma mogu biti dobijene različitim metodama magnetronskog raspršivanja, pri čemu se različite varijante ove metode mogu uspešno kombinovati. Zbog svega toga u ovom radu je dat pregled ovih metoda, sa njihovim glavnim karakteristikama i nedostacima. Magnetronsko nanošenje raspršivanjem uključuje površinsku modifikaciju SMA u jednoslojnim, višeslojnim, gradiranim slojevima i nanokompozitne tanke prevlake za dobijanje sistema sa superiornim "funkcionalnim" karakteristikama, kao što su vrlo visoka tvrdoća, otpornost na habanje, abraziju i eroziju, poboljšano prijanjanje na različite tehnološki važne supstratne materijale kao što su polimeri, hidrofobnost ili hidrofilnost, dugotrajna hemijska, termička i ekološka stabilnost, nepropusnost gasa i para i drugi. Ovaj rad predstavlja neku vrstu kritičkog pregleda napretka u razvoju magnetronskog raspršivanja modifikovanih SMA proizvoda u stomatologiji, sa unapred predvidljivim fizičko-hemijskim, strukturnim i antimikrobnim osobinama.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics, Metode magnetronskog raspršivanja za površinsku modifikaciju memorijskih legura za primenu u ortodonciji i endodonciji",
volume = "66",
number = "2",
pages = "87-96",
doi = "10.2478/sdj-2019-0010"
}
Jokanović, V., Čolović, B., Ćetenović, B.,& Živković, S.. (2019). Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 66(2), 87-96.
https://doi.org/10.2478/sdj-2019-0010
Jokanović V, Čolović B, Ćetenović B, Živković S. Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics. in Stomatološki glasnik Srbije. 2019;66(2):87-96.
doi:10.2478/sdj-2019-0010 .
Jokanović, Vukoman, Čolović, Božana, Ćetenović, Bojana, Živković, Slavoljub, "Magnetron sputtering methods for surface modification of shape memory alloys for applications in orthodontics and endodontics" in Stomatološki glasnik Srbije, 66, no. 2 (2019):87-96,
https://doi.org/10.2478/sdj-2019-0010 . .

Antibacterial effects of new endodontic materials based on calcium silicates

Trišić, Dijana; Ćetenović, Bojana; Zdravković, Nemanja; Marković, Tatjana; Dojčinović, Biljana; Jokanović, Vukoman; Marković, Dejan

(Vojnomedicinska akademija - Institut za naučne informacije, Beograd, 2019)

TY  - JOUR
AU  - Trišić, Dijana
AU  - Ćetenović, Bojana
AU  - Zdravković, Nemanja
AU  - Marković, Tatjana
AU  - Dojčinović, Biljana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2444
AB  - Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution.
AB  - Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Antibacterial effects of new endodontic materials based on calcium silicates
T1  - Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata
VL  - 76
IS  - 4
SP  - 365
EP  - 372
DO  - 10.2298/VSP161231130T
ER  - 
@article{
author = "Trišić, Dijana and Ćetenović, Bojana and Zdravković, Nemanja and Marković, Tatjana and Dojčinović, Biljana and Jokanović, Vukoman and Marković, Dejan",
year = "2019",
abstract = "Background/Aim. The main task of endodontic treatment is to eliminate pathologically altered tissue, to disinfect root canal space and to obtain its three-dimensional hermetic obturation. The main purpose of this study was to evaluate antimicrobial activity of new endodontic nano-structured highly active calcium silicates based materials albo-mineral plyoxide carbonate aggregate (ALBO-MPCA) and calcium silicates (CS) in comparison to mineral trioxide aggregate (MTA +) and UltraCal XS (CH). Methods. The antimicrobial activity of materials was tested against Staphylococcus aureus (ATCC 25923) and Entero-coccus faecalis (ATCC 14506) strains, and following clinical isolates: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus and Streptococcus vestibularis using a double layer agar diffusion test. The pH measurements were performed using the pH meter. Total amount of released ions was determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Results. All tested materials showed the best antibacterial potential after 1 h of incubation. After 3h and 24h of the incubation period, the an-tibacterial potential of all tested materials were similar. The Agar diffusion test showed that ALBO-MPCA, CS and MTA + had similar inhibition zones (p > 0.05), except in the activity against Staphylococcus aureus where ALBO-MPCA showed better antimicrobial properties than MTA + in 3h and 24h of the incubation period (p  lt  0.05). Following 24h of the incubation, the inhibition zones were the strongest with CH against Staphylococcus aureus (16.67 ± 2.34 mm) followed by ALBO-MPCA (14.67 ± 1.21 mm) and the weakest with CS against Enterococcus faecalis (6.50 ± 1.76 mm). CH showed the highest pH, followed by ALBO-MPCA, CS and MTA +. Conclusion. The expressed antibacterial effects indicate that materials based on nano-structured highly active calcium silicates represent effective therapeutic agents for root canal obturation in one-visit apexification treatment, therefore they are recommend for further examination and clinical trials as they are proposed for MTA substitution., Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA +) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylococcus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vred-nosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBOMPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p  lt  0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slučaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBOMPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Antibacterial effects of new endodontic materials based on calcium silicates, Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata",
volume = "76",
number = "4",
pages = "365-372",
doi = "10.2298/VSP161231130T"
}
Trišić, D., Ćetenović, B., Zdravković, N., Marković, T., Dojčinović, B., Jokanović, V.,& Marković, D.. (2019). Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 76(4), 365-372.
https://doi.org/10.2298/VSP161231130T
Trišić D, Ćetenović B, Zdravković N, Marković T, Dojčinović B, Jokanović V, Marković D. Antibacterial effects of new endodontic materials based on calcium silicates. in Vojnosanitetski pregled. 2019;76(4):365-372.
doi:10.2298/VSP161231130T .
Trišić, Dijana, Ćetenović, Bojana, Zdravković, Nemanja, Marković, Tatjana, Dojčinović, Biljana, Jokanović, Vukoman, Marković, Dejan, "Antibacterial effects of new endodontic materials based on calcium silicates" in Vojnosanitetski pregled, 76, no. 4 (2019):365-372,
https://doi.org/10.2298/VSP161231130T . .
1

Physico-chemical and biological properties of dental calcium silicate cements - literature review

Ilić, Dragan; Antonijević, Đorđe; Biočanin, Vladimir; Čolović, Božana; Danilović, Vesna; Komlev, Vladimir S.; Teterina, Anastasia; Jokanović, Vukoman

(Savez hemijskih inženjera, Beograd, 2019)

TY  - JOUR
AU  - Ilić, Dragan
AU  - Antonijević, Đorđe
AU  - Biočanin, Vladimir
AU  - Čolović, Božana
AU  - Danilović, Vesna
AU  - Komlev, Vladimir S.
AU  - Teterina, Anastasia
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2411
AB  - Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Physico-chemical and biological properties of dental calcium silicate cements - literature review
VL  - 73
IS  - 5
SP  - 281
EP  - 294
DO  - 10.2298/HEMIND190614027I
ER  - 
@article{
author = "Ilić, Dragan and Antonijević, Đorđe and Biočanin, Vladimir and Čolović, Božana and Danilović, Vesna and Komlev, Vladimir S. and Teterina, Anastasia and Jokanović, Vukoman",
year = "2019",
abstract = "Dental cement materials have been developed with the aim to replace hard dental tissues. The first material used for pulp capping, root canal obturation, bifurcation perforation and apexification is calcium hydroxide (in 1920). A half century later, glass-ionomer cements began to suppress it as dentine substitutes. Finally, in the 1990s, calcium silicate (CS) material appeared in the dental research community as the most promising dentine substitute capable to adequately meet all clinical requirements. The aim of this paper is to present an overview of literature related to studies about CS materials taking into account their physical, chemical and biological properties and clinical applications. This review aims to discuss beneficial and adverse characteristics of CSs concerning interactions to the hard dentine and soft pulp/periodontal tissues. This review article deals with the literature data about currently commercially available CS concerning laboratory and clinical findings. 109 scientific articles were analyzed of which 62 references reported in vitro and 26 in vivo investigations while 21 references comprised reports, reviews and books dealing with both, in vitro and in vivo investigations. Although further data collection is necessary, CSs are promising materials that represent a gold standard for numerous dental clinical procedures.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Physico-chemical and biological properties of dental calcium silicate cements - literature review",
volume = "73",
number = "5",
pages = "281-294",
doi = "10.2298/HEMIND190614027I"
}
Ilić, D., Antonijević, Đ., Biočanin, V., Čolović, B., Danilović, V., Komlev, V. S., Teterina, A.,& Jokanović, V.. (2019). Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 73(5), 281-294.
https://doi.org/10.2298/HEMIND190614027I
Ilić D, Antonijević Đ, Biočanin V, Čolović B, Danilović V, Komlev VS, Teterina A, Jokanović V. Physico-chemical and biological properties of dental calcium silicate cements - literature review. in Hemijska Industrija. 2019;73(5):281-294.
doi:10.2298/HEMIND190614027I .
Ilić, Dragan, Antonijević, Đorđe, Biočanin, Vladimir, Čolović, Božana, Danilović, Vesna, Komlev, Vladimir S., Teterina, Anastasia, Jokanović, Vukoman, "Physico-chemical and biological properties of dental calcium silicate cements - literature review" in Hemijska Industrija, 73, no. 5 (2019):281-294,
https://doi.org/10.2298/HEMIND190614027I . .
2
1
4

Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis

Paras, S.; Janković, Ognjenka; Trišić, Dijana; Čolović, Božana; Mitrović-Ajtić, O.; Dekić, R.; Soldatović, Ivan; Živković-Sandić, Marija; Živković, Slavoljub; Jokanović, Vukoman

(Wiley, Hoboken, 2019)

TY  - JOUR
AU  - Paras, S.
AU  - Janković, Ognjenka
AU  - Trišić, Dijana
AU  - Čolović, Božana
AU  - Mitrović-Ajtić, O.
AU  - Dekić, R.
AU  - Soldatović, Ivan
AU  - Živković-Sandić, Marija
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2393
AB  - Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis
VL  - 52
IS  - 8
SP  - 1162
EP  - 1172
DO  - 10.1111/iej.13105
ER  - 
@article{
author = "Paras, S. and Janković, Ognjenka and Trišić, Dijana and Čolović, Božana and Mitrović-Ajtić, O. and Dekić, R. and Soldatović, Ivan and Živković-Sandić, Marija and Živković, Slavoljub and Jokanović, Vukoman",
year = "2019",
abstract = "Aim To examine the potential systemic toxicity of nanostructured materials based on calcium silicate and calcium aluminate, for potential application in Dentistry. Methodology Twenty-four Albino Wistar rats aged 2 months were used as an in vivo animal model for subcutaneous implantation of the investigated materials, placed in polyethylene tubes. Thirty days after implantation, the livers of the rats were analysed and following histological and stereological parameters were evaluated for volume density of hepatocytes and blood sinusoids, number and numerical density of hepatocytes, surface of hepatocytes and their nucleuses, nucleocytoplasmic ratio and mitotic index of hepatocytes. Stereological measurements were achieved using Cavalieri's principle, with grid P2 and unbiased analysis. Additionally, immunohistochemistry studies were performed to further analyse changes in liver tissue. Several haematological and biochemical parameters of blood of experimental animals were also analysed, as well as local tissue reactions around the implants. Statistical analysis was performed using parametric (anova and t-test) and nonparametric tests (Kruskal-Wallis and Mann-Whitney U-test) depending on data distribution. Results Implanted dental cements led to an increase in stereological and histological parameters in liver tissue compared to control rats. Although the investigated parameters mostly showed significant differences between control and experimental animals, the liver tissue of the experimental animals did not have visible signs of pathological changes. This was supported by the analysis of blood parameters which were not significantly different between control and experimental animals. Also, the subcutaneous tissues had minimal inflammatory reactions. Immunohistochemistry studies revealed that nanostructured materials induced proliferation of hepatocytes, but that the immunological response to the materials was not strong enough to induce proliferation of immunoreactive cells in liver in the observed time period. Conclusions This study was performed as a contribution to the attestation of the biocompatibility of dental cements based on calcium silicate and calcium aluminate. Although these materials induced several changes in the liver structure, they were not clinically relevant and represent a normal and reversible response of the liver to the presence of biocompatible materials in the body. Blood and immunohistochemistry analyses and local tissue reactions further confirmed that these materials possess good biocompatible potential.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis",
volume = "52",
number = "8",
pages = "1162-1172",
doi = "10.1111/iej.13105"
}
Paras, S., Janković, O., Trišić, D., Čolović, B., Mitrović-Ajtić, O., Dekić, R., Soldatović, I., Živković-Sandić, M., Živković, S.,& Jokanović, V.. (2019). Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal
Wiley, Hoboken., 52(8), 1162-1172.
https://doi.org/10.1111/iej.13105
Paras S, Janković O, Trišić D, Čolović B, Mitrović-Ajtić O, Dekić R, Soldatović I, Živković-Sandić M, Živković S, Jokanović V. Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis. in International Endodontic Journal. 2019;52(8):1162-1172.
doi:10.1111/iej.13105 .
Paras, S., Janković, Ognjenka, Trišić, Dijana, Čolović, Božana, Mitrović-Ajtić, O., Dekić, R., Soldatović, Ivan, Živković-Sandić, Marija, Živković, Slavoljub, Jokanović, Vukoman, "Influence of nanostructured calcium aluminate and calcium silicate on the liver: histological and unbiased stereological analysis" in International Endodontic Journal, 52, no. 8 (2019):1162-1172,
https://doi.org/10.1111/iej.13105 . .
9
6
9

Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite

Opačić-Galić, Vanja; Petrović, Violeta; Popović-Bajić, Marijana; Jokanović, Vukoman; Živković, Slavoljub; Nikolić, Biljana; Mitić-Ćulafić, Dragana

(Springer International Publishing, 2018)

TY  - CHAP
AU  - Opačić-Galić, Vanja
AU  - Petrović, Violeta
AU  - Popović-Bajić, Marijana
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
AU  - Nikolić, Biljana
AU  - Mitić-Ćulafić, Dragana
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2371
PB  - Springer International Publishing
T2  - Dental Applications of Nanotechnology
T1  - Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite
SP  - 247
EP  - 271
DO  - 10.1007/978-3-319-97634-1_13
ER  - 
@inbook{
author = "Opačić-Galić, Vanja and Petrović, Violeta and Popović-Bajić, Marijana and Jokanović, Vukoman and Živković, Slavoljub and Nikolić, Biljana and Mitić-Ćulafić, Dragana",
year = "2018",
publisher = "Springer International Publishing",
journal = "Dental Applications of Nanotechnology",
booktitle = "Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite",
pages = "247-271",
doi = "10.1007/978-3-319-97634-1_13"
}
Opačić-Galić, V., Petrović, V., Popović-Bajić, M., Jokanović, V., Živković, S., Nikolić, B.,& Mitić-Ćulafić, D.. (2018). Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. in Dental Applications of Nanotechnology
Springer International Publishing., 247-271.
https://doi.org/10.1007/978-3-319-97634-1_13
Opačić-Galić V, Petrović V, Popović-Bajić M, Jokanović V, Živković S, Nikolić B, Mitić-Ćulafić D. Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite. in Dental Applications of Nanotechnology. 2018;:247-271.
doi:10.1007/978-3-319-97634-1_13 .
Opačić-Galić, Vanja, Petrović, Violeta, Popović-Bajić, Marijana, Jokanović, Vukoman, Živković, Slavoljub, Nikolić, Biljana, Mitić-Ćulafić, Dragana, "Physical properties and biocompatibility of nanostructural biomaterials based on active calcium silicate systems and hydroxyapatite" in Dental Applications of Nanotechnology (2018):247-271,
https://doi.org/10.1007/978-3-319-97634-1_13 . .
2
1

Stem cells in tissue engineering: Dynamic cultivation requirement

Trišić, Dijana; Jokanović, Vukoman; Antonijević, Đorđe; Marković, Dejan

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2018)

TY  - JOUR
AU  - Trišić, Dijana
AU  - Jokanović, Vukoman
AU  - Antonijević, Đorđe
AU  - Marković, Dejan
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2369
AB  - Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells' niche from in vivo conditions. In an attempt to provide biomimetic microenvironments for stem cells in vitro growth, three-dimensional culture techniques have been developed. In our study advantages of newly developed porous scaffolds as the most promising in vitro imitation of niche that provides physical support, enables cell growth, regeneration and neovascularization, while they are replaced in time with newly created tissue was explained. Furthermore, dynamic cultivation techniques have been described, as new way of cell culturing that will be the main subject of our future research. In that manner, by developing an optimal dynamic culturing method, high-quality new cells and tissues would be possible to obtain, for any future clinical application.
AB  - Matične ćelije su pokazale veliki potencijal za primenu u tkivnom inženjerstvu u in vitro uslovima, regenerativnoj medicini, lečenju matičnim ćelijama i farmaceutskoj primeni. Sve ove grane, posebno primena u kliničkim istraživanjima, zahtevaće kontrolisano stvaranje visokokvalitetnih ćelija. Tradicionalnim tehnikama izolovana je većina primarnih ćelijskih kultura i ćelijskih linija, i vremenom su one postale standardne tehnike za različite ćelijske analize. Ipak, ovi uslovi gajenja nisu u mogućnosti da imitiraju dinamične, trodimenzionalne uslove mikrosredine niše matičnih ćelija iz in vivo uslova. U pokušaju da se obezbede navedeni uslovi i u in vitro uzgajanju ćelija, razvile su se trodimenzionalne tehnike gajenja ćelija. U ovom preglednom radu opisujemo prednosti novorazvijenih poroznih nosača ćelija, kao ključnih činilaca u imitaciji ćelijske niše koji obezbeđuju mehaničku potporu, omogućuju rast ćelija, regeneraciju i razvoj novih krvnih sudova, dok vremenom bivaju razgrađeni i zamenjeni novostvorenim tkivom. Dalje, tehnike dinamičnog gajenja ćelija su opisane kao vid novog načina gajenja ćelijskih kultura koji predstavlja i pravac naših budućih istraživanja. U tom smislu, razvijajući optimalan, dinamički model gajenja ćelija, biće moguće obezbediti nove ćelije i tkiva visokog kvaliteta za sva dalja klinička istraživanja.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Stem cells in tissue engineering: Dynamic cultivation requirement
T1  - Matične ćelije u tkivnom inženjerstvu - potreba za dinamičnom kultivacijom
VL  - 65
IS  - 1
SP  - 37
EP  - 44
DO  - 10.2478/sdj-2018-0005
ER  - 
@article{
author = "Trišić, Dijana and Jokanović, Vukoman and Antonijević, Đorđe and Marković, Dejan",
year = "2018",
abstract = "Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells' niche from in vivo conditions. In an attempt to provide biomimetic microenvironments for stem cells in vitro growth, three-dimensional culture techniques have been developed. In our study advantages of newly developed porous scaffolds as the most promising in vitro imitation of niche that provides physical support, enables cell growth, regeneration and neovascularization, while they are replaced in time with newly created tissue was explained. Furthermore, dynamic cultivation techniques have been described, as new way of cell culturing that will be the main subject of our future research. In that manner, by developing an optimal dynamic culturing method, high-quality new cells and tissues would be possible to obtain, for any future clinical application., Matične ćelije su pokazale veliki potencijal za primenu u tkivnom inženjerstvu u in vitro uslovima, regenerativnoj medicini, lečenju matičnim ćelijama i farmaceutskoj primeni. Sve ove grane, posebno primena u kliničkim istraživanjima, zahtevaće kontrolisano stvaranje visokokvalitetnih ćelija. Tradicionalnim tehnikama izolovana je većina primarnih ćelijskih kultura i ćelijskih linija, i vremenom su one postale standardne tehnike za različite ćelijske analize. Ipak, ovi uslovi gajenja nisu u mogućnosti da imitiraju dinamične, trodimenzionalne uslove mikrosredine niše matičnih ćelija iz in vivo uslova. U pokušaju da se obezbede navedeni uslovi i u in vitro uzgajanju ćelija, razvile su se trodimenzionalne tehnike gajenja ćelija. U ovom preglednom radu opisujemo prednosti novorazvijenih poroznih nosača ćelija, kao ključnih činilaca u imitaciji ćelijske niše koji obezbeđuju mehaničku potporu, omogućuju rast ćelija, regeneraciju i razvoj novih krvnih sudova, dok vremenom bivaju razgrađeni i zamenjeni novostvorenim tkivom. Dalje, tehnike dinamičnog gajenja ćelija su opisane kao vid novog načina gajenja ćelijskih kultura koji predstavlja i pravac naših budućih istraživanja. U tom smislu, razvijajući optimalan, dinamički model gajenja ćelija, biće moguće obezbediti nove ćelije i tkiva visokog kvaliteta za sva dalja klinička istraživanja.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Stem cells in tissue engineering: Dynamic cultivation requirement, Matične ćelije u tkivnom inženjerstvu - potreba za dinamičnom kultivacijom",
volume = "65",
number = "1",
pages = "37-44",
doi = "10.2478/sdj-2018-0005"
}
Trišić, D., Jokanović, V., Antonijević, Đ.,& Marković, D.. (2018). Stem cells in tissue engineering: Dynamic cultivation requirement. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(1), 37-44.
https://doi.org/10.2478/sdj-2018-0005
Trišić D, Jokanović V, Antonijević Đ, Marković D. Stem cells in tissue engineering: Dynamic cultivation requirement. in Stomatološki glasnik Srbije. 2018;65(1):37-44.
doi:10.2478/sdj-2018-0005 .
Trišić, Dijana, Jokanović, Vukoman, Antonijević, Đorđe, Marković, Dejan, "Stem cells in tissue engineering: Dynamic cultivation requirement" in Stomatološki glasnik Srbije, 65, no. 1 (2018):37-44,
https://doi.org/10.2478/sdj-2018-0005 . .

Compressive strength of calcium silicate-based cement

Opačić-Galić, Vanja; Stamenić, Zoran; Petrović, Violeta; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2018)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Stamenić, Zoran
AU  - Petrović, Violeta
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2358
AB  - Introduction The aim of this study was to compare compressive strength (Cs) of new nanostructural calcium silicate based cement (nCS) with commercial calcium silicate cement and conventional GIC. Methods Four nanostructural materials were tested: nanostructural calcium silicate based cement (nCS) (Jokanović et al.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Five samples of each material were mixed in accordance with manifecturer's guidelines and positioned in metal moulds (ϕ4mm and 6mm). Compressive strength (Cs) expressed in MPa was determined after 24 hours, 7 days and 28 days respectively. Measurements were performed on universal testing equipment (Tinius Olsen, USA) at a crosshead speed of 1mm/min. For processing the results one-way ANOVA and post-hoc test were used. Results The highest values of compressive strength after 24h was found in conventional GIC Fuji IX (mean 38.56±13.31) and Ketac Universal (mean 40.77±7.96). Calcium silicate cements after 24h showed low values of compressive strength (MTA Plus 5.91±0.28 MPa, nCS 1.35±0.36 MPa). After 7 days, FUJI IX 47.42±9.33 MPa and Ketac Universal 35.25±10.60 MPa showed higher value of compressive strength than MTA Plus (15.09±2.77 MPa) and nCS (11.06±0.88 MPa). After 28 days the Cs value for conventional GIC Fuji IX was 48.03±7.82 MPa and Ketac Universal 36.65±11.13 MPa while for calcium silicate cements it was 16.47±1.89 MPa and nCS 14.39±1.63 MPa. There was statistically significant difference (p lt 0.05) in Cs between conventional GIC and CS cements after 24h, 7 and 28 days. Conclusions Calcium silicate cements initially showed lower values of compressive strength than conventional GIC that increased over time.
AB  - Uvod Cilj ovog rada je bio da se proveri kompresivna čvrstoća (KČ) novog nanostrukturnog kalcijum-silikatnog cementa (nCS) i uporedi sa komercijalnim kalcijum-silikatnim cementom i konvencionalnim GJC u funkciji vremena. Materijal i metod Testirana su četiri materijala - nanostrukturni CS (Jokanović i sar.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Po pet uzoraka za svaki materijal je zamešano po proizvođačkom uputstvu i postavljano u metalne kalupe (ϕ 4 mm i 6 mm visoke). KČ, izražena u megapaskalima, merena je posle 24 sata i posle 7 i 28 dana na univerzalnoj test mašini (Tinius Olsen, USA) sa brzinom utiskivača od 1 mm/min. Dobijeni rezultati su statistički obrađeni one-way ANOVA i post hoc Tukey, s testovima. Resultati Posle 24 sata najveću KČ imao je FUJI IX (38,56 ± 13,31 MPa), zatim Ketac Univerzal (40,77 ± 7,96 MPa). Kalcijum-silikatni cementi su pokazali niže vrednosti KČ 24 sata posle mešanja i to MTA 5,91 ± 0,28, a nCS 1,35 ± 0,36 MPa. Posle sedam dana KČ za FUJI IX je bila 47,72 ± 9,33 MPa, a za Ketac Universal 35,25 ± 10,60 MPa, dok je vrednost za MTA bila 15,09 ± 2,77 MPa, a nCS 11,06 ± 0,88. Posle 28 dana KČ za FUJI IX je bila 48,03 ± 7,82 MPa, a za Ketac 36,65 ± 11,13 MPa. KČ kalcijum-silikatnih cemenata posle 28 dana je bila 16,47 ± 1,89 za MTA, a za nCS 14,39 ± 1,63 MPa, bez statističi značajne razlike između njih. Između konvencionalnih GJC i CS cemenata postoji statistički značajna razlika (p  lt  0,05) posle 24 h, kao i posle 7 i 28 dana. Zaključak Kalcijum-silikatni cementi su inicijalno pokazali niže vrednosti KČ u odnosu na konvencionalne GJC, ali su se one povećavale u funkciji vremena.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Compressive strength of calcium silicate-based cement
T1  - Ispitivanje kompresivne čvrstoće kalcijum-silikatnih cemenata
VL  - 65
IS  - 1
SP  - 7
EP  - 13
DO  - 10.2478/sdj-2018-0001
ER  - 
@article{
author = "Opačić-Galić, Vanja and Stamenić, Zoran and Petrović, Violeta and Jokanović, Vukoman and Živković, Slavoljub",
year = "2018",
abstract = "Introduction The aim of this study was to compare compressive strength (Cs) of new nanostructural calcium silicate based cement (nCS) with commercial calcium silicate cement and conventional GIC. Methods Four nanostructural materials were tested: nanostructural calcium silicate based cement (nCS) (Jokanović et al.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Five samples of each material were mixed in accordance with manifecturer's guidelines and positioned in metal moulds (ϕ4mm and 6mm). Compressive strength (Cs) expressed in MPa was determined after 24 hours, 7 days and 28 days respectively. Measurements were performed on universal testing equipment (Tinius Olsen, USA) at a crosshead speed of 1mm/min. For processing the results one-way ANOVA and post-hoc test were used. Results The highest values of compressive strength after 24h was found in conventional GIC Fuji IX (mean 38.56±13.31) and Ketac Universal (mean 40.77±7.96). Calcium silicate cements after 24h showed low values of compressive strength (MTA Plus 5.91±0.28 MPa, nCS 1.35±0.36 MPa). After 7 days, FUJI IX 47.42±9.33 MPa and Ketac Universal 35.25±10.60 MPa showed higher value of compressive strength than MTA Plus (15.09±2.77 MPa) and nCS (11.06±0.88 MPa). After 28 days the Cs value for conventional GIC Fuji IX was 48.03±7.82 MPa and Ketac Universal 36.65±11.13 MPa while for calcium silicate cements it was 16.47±1.89 MPa and nCS 14.39±1.63 MPa. There was statistically significant difference (p lt 0.05) in Cs between conventional GIC and CS cements after 24h, 7 and 28 days. Conclusions Calcium silicate cements initially showed lower values of compressive strength than conventional GIC that increased over time., Uvod Cilj ovog rada je bio da se proveri kompresivna čvrstoća (KČ) novog nanostrukturnog kalcijum-silikatnog cementa (nCS) i uporedi sa komercijalnim kalcijum-silikatnim cementom i konvencionalnim GJC u funkciji vremena. Materijal i metod Testirana su četiri materijala - nanostrukturni CS (Jokanović i sar.), MTA Plus (Cerkamed, Poland), Fuji IX (GC Corporation, Japan) and Ketac Universal Aplicap (3M ESPE, USA). Po pet uzoraka za svaki materijal je zamešano po proizvođačkom uputstvu i postavljano u metalne kalupe (ϕ 4 mm i 6 mm visoke). KČ, izražena u megapaskalima, merena je posle 24 sata i posle 7 i 28 dana na univerzalnoj test mašini (Tinius Olsen, USA) sa brzinom utiskivača od 1 mm/min. Dobijeni rezultati su statistički obrađeni one-way ANOVA i post hoc Tukey, s testovima. Resultati Posle 24 sata najveću KČ imao je FUJI IX (38,56 ± 13,31 MPa), zatim Ketac Univerzal (40,77 ± 7,96 MPa). Kalcijum-silikatni cementi su pokazali niže vrednosti KČ 24 sata posle mešanja i to MTA 5,91 ± 0,28, a nCS 1,35 ± 0,36 MPa. Posle sedam dana KČ za FUJI IX je bila 47,72 ± 9,33 MPa, a za Ketac Universal 35,25 ± 10,60 MPa, dok je vrednost za MTA bila 15,09 ± 2,77 MPa, a nCS 11,06 ± 0,88. Posle 28 dana KČ za FUJI IX je bila 48,03 ± 7,82 MPa, a za Ketac 36,65 ± 11,13 MPa. KČ kalcijum-silikatnih cemenata posle 28 dana je bila 16,47 ± 1,89 za MTA, a za nCS 14,39 ± 1,63 MPa, bez statističi značajne razlike između njih. Između konvencionalnih GJC i CS cemenata postoji statistički značajna razlika (p  lt  0,05) posle 24 h, kao i posle 7 i 28 dana. Zaključak Kalcijum-silikatni cementi su inicijalno pokazali niže vrednosti KČ u odnosu na konvencionalne GJC, ali su se one povećavale u funkciji vremena.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Compressive strength of calcium silicate-based cement, Ispitivanje kompresivne čvrstoće kalcijum-silikatnih cemenata",
volume = "65",
number = "1",
pages = "7-13",
doi = "10.2478/sdj-2018-0001"
}
Opačić-Galić, V., Stamenić, Z., Petrović, V., Jokanović, V.,& Živković, S.. (2018). Compressive strength of calcium silicate-based cement. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 65(1), 7-13.
https://doi.org/10.2478/sdj-2018-0001
Opačić-Galić V, Stamenić Z, Petrović V, Jokanović V, Živković S. Compressive strength of calcium silicate-based cement. in Stomatološki glasnik Srbije. 2018;65(1):7-13.
doi:10.2478/sdj-2018-0001 .
Opačić-Galić, Vanja, Stamenić, Zoran, Petrović, Violeta, Jokanović, Vukoman, Živković, Slavoljub, "Compressive strength of calcium silicate-based cement" in Stomatološki glasnik Srbije, 65, no. 1 (2018):7-13,
https://doi.org/10.2478/sdj-2018-0001 . .

Biocompatibility of nanostructured biomaterials based on calcium aluminate

Janković, Ognjenka; Paraš, Smiljana; Tadić-Latinović, Ljiljana; Josipović, Renata; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo, Beograd, 2018)

TY  - JOUR
AU  - Janković, Ognjenka
AU  - Paraš, Smiljana
AU  - Tadić-Latinović, Ljiljana
AU  - Josipović, Renata
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2352
AB  - Introduction/Objective The aim of this paper was to verify the biocompatibility of the newly synthesized nanostructured material based on calcium aluminate after implantation into the subcutaneous tissue of rats. Methods The study included 18 rats aged 10-11 weeks, divided into two experimental groups (n = 9). In all animals, incision took place on the back and two pockets of 15 mm in depth were made, in which sterile polyethylene tubes with test materials [calcium aluminate cement (ALBO-CA), calcium silicate cement with the addition of hydroxyapatite (ALBO-CSHA), and mineral trioxide aggregate (MTA) for the control group) were implanted. Six rats of each group were sacrificed in three observational periods (seven, 15, 30 days). Pathohistological analysis included inflammation, bleeding, fibrous capsule, and tissue integrity around the implanted material. Results After seven days of treatment, ALBO-CA and ALBO-CSHA showed better tissue response compared to MTA, with a statistically significant difference in inflammation intensity (p = 0.2781). The difference in vascular congestion and thickness of the fibrous capsule after implantation of ALBO-CA material compared to MTA was also statistically significant (p = 0.5567). At the end of the 30-day evaluation period, an identical inflammatory response of connective tissue at the site of implanting ALBO-CA, ALBO-CSHA, and MTA (score of 0.7) was recorded. The formation of thick or moderately thick fibrous capsule was found to be the thickest in ALBO-CA (grade 3.7). There were no statistically significant differences between the parameters analyzed after 30 days. Conclusion Newly synthesized ALBO-CA showed a satisfactory tissue response and confirmed biocompatibility after implantation in subcutaneous tissue of rats.
AB  - Uvod/Cilj Cilj ovog rada je bio da se proveri biokompatibilnost novosintetisanog nanostrukturnog materijala na bazi kalcijum-aluminata posle implantacije u potkožno tkivo pacova. Metode U istraživanje je uključeno 18 pacova starosti 10-11 sedmica, koji su podeljeni u dve eksperimentalne grupe (n = 9). Kod svih životinja je urađena incizija na leđima i formirana su dva džepa dubine 15 mm u koja su aplicirane sterilne polietilenske tubice sa testnim materijalima (ALBO-CA, ALBO-CSHA i kontrola MTA). Po šest pacova svake grupe je žrtvovano u tri opservaciona perioda (7, 15 i 30 dana). Patohistološki su analizirani inflamacija, krvarenje, fibrozna kapsula i integritet tkiva oko implantiranog materijala. Rezultati Posle sedam dana ALBO-CA i ALBO-CSHA su pokazali bolji tkivni odgovor u odnosu na MTA sa statistički značajnom razlikom u intenzitetu inflamacije (p = 0,2781). Razlika u vaskularnoj kongestiji i debljini fibrozne kapsule posle implantacije materijala ALBO-CA u odnosu na MTA je takođe bila statistički značajna (p = 0,5567). Na kraju evaluacionog perioda od 30 dana zabeležen je identičan inflamatorni odgovor rastresitog vezivnog tkiva na mestu implantacije ALBO-CA, ALBO-CSHA i MTA (ocena 0,7). Konstatovano je formiranje debele ili umereno debele fibrozne kapsule, koja je bila najdeblja kod ALBO-CA (ocena 3,7). Statistički značajne razlike između analiziranih parametara posle 30 dana nije bilo. Zaključak Novosintetisani ALBO-CA je pokazao zadovoljavajuću tkivnu reakciju i potvrdio biokompatibilnost posle implantacije u potkožno tkivo pacova.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Biocompatibility of nanostructured biomaterials based on calcium aluminate
T1  - Biokompatibilnost nanostrukturnih biomaterijala na bazi kalcijum-aluminata
VL  - 146
IS  - 11-12
SP  - 634
EP  - 640
DO  - 10.2298/SARH171211030J
ER  - 
@article{
author = "Janković, Ognjenka and Paraš, Smiljana and Tadić-Latinović, Ljiljana and Josipović, Renata and Jokanović, Vukoman and Živković, Slavoljub",
year = "2018",
abstract = "Introduction/Objective The aim of this paper was to verify the biocompatibility of the newly synthesized nanostructured material based on calcium aluminate after implantation into the subcutaneous tissue of rats. Methods The study included 18 rats aged 10-11 weeks, divided into two experimental groups (n = 9). In all animals, incision took place on the back and two pockets of 15 mm in depth were made, in which sterile polyethylene tubes with test materials [calcium aluminate cement (ALBO-CA), calcium silicate cement with the addition of hydroxyapatite (ALBO-CSHA), and mineral trioxide aggregate (MTA) for the control group) were implanted. Six rats of each group were sacrificed in three observational periods (seven, 15, 30 days). Pathohistological analysis included inflammation, bleeding, fibrous capsule, and tissue integrity around the implanted material. Results After seven days of treatment, ALBO-CA and ALBO-CSHA showed better tissue response compared to MTA, with a statistically significant difference in inflammation intensity (p = 0.2781). The difference in vascular congestion and thickness of the fibrous capsule after implantation of ALBO-CA material compared to MTA was also statistically significant (p = 0.5567). At the end of the 30-day evaluation period, an identical inflammatory response of connective tissue at the site of implanting ALBO-CA, ALBO-CSHA, and MTA (score of 0.7) was recorded. The formation of thick or moderately thick fibrous capsule was found to be the thickest in ALBO-CA (grade 3.7). There were no statistically significant differences between the parameters analyzed after 30 days. Conclusion Newly synthesized ALBO-CA showed a satisfactory tissue response and confirmed biocompatibility after implantation in subcutaneous tissue of rats., Uvod/Cilj Cilj ovog rada je bio da se proveri biokompatibilnost novosintetisanog nanostrukturnog materijala na bazi kalcijum-aluminata posle implantacije u potkožno tkivo pacova. Metode U istraživanje je uključeno 18 pacova starosti 10-11 sedmica, koji su podeljeni u dve eksperimentalne grupe (n = 9). Kod svih životinja je urađena incizija na leđima i formirana su dva džepa dubine 15 mm u koja su aplicirane sterilne polietilenske tubice sa testnim materijalima (ALBO-CA, ALBO-CSHA i kontrola MTA). Po šest pacova svake grupe je žrtvovano u tri opservaciona perioda (7, 15 i 30 dana). Patohistološki su analizirani inflamacija, krvarenje, fibrozna kapsula i integritet tkiva oko implantiranog materijala. Rezultati Posle sedam dana ALBO-CA i ALBO-CSHA su pokazali bolji tkivni odgovor u odnosu na MTA sa statistički značajnom razlikom u intenzitetu inflamacije (p = 0,2781). Razlika u vaskularnoj kongestiji i debljini fibrozne kapsule posle implantacije materijala ALBO-CA u odnosu na MTA je takođe bila statistički značajna (p = 0,5567). Na kraju evaluacionog perioda od 30 dana zabeležen je identičan inflamatorni odgovor rastresitog vezivnog tkiva na mestu implantacije ALBO-CA, ALBO-CSHA i MTA (ocena 0,7). Konstatovano je formiranje debele ili umereno debele fibrozne kapsule, koja je bila najdeblja kod ALBO-CA (ocena 3,7). Statistički značajne razlike između analiziranih parametara posle 30 dana nije bilo. Zaključak Novosintetisani ALBO-CA je pokazao zadovoljavajuću tkivnu reakciju i potvrdio biokompatibilnost posle implantacije u potkožno tkivo pacova.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Biocompatibility of nanostructured biomaterials based on calcium aluminate, Biokompatibilnost nanostrukturnih biomaterijala na bazi kalcijum-aluminata",
volume = "146",
number = "11-12",
pages = "634-640",
doi = "10.2298/SARH171211030J"
}
Janković, O., Paraš, S., Tadić-Latinović, L., Josipović, R., Jokanović, V.,& Živković, S.. (2018). Biocompatibility of nanostructured biomaterials based on calcium aluminate. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 146(11-12), 634-640.
https://doi.org/10.2298/SARH171211030J
Janković O, Paraš S, Tadić-Latinović L, Josipović R, Jokanović V, Živković S. Biocompatibility of nanostructured biomaterials based on calcium aluminate. in Srpski arhiv za celokupno lekarstvo. 2018;146(11-12):634-640.
doi:10.2298/SARH171211030J .
Janković, Ognjenka, Paraš, Smiljana, Tadić-Latinović, Ljiljana, Josipović, Renata, Jokanović, Vukoman, Živković, Slavoljub, "Biocompatibility of nanostructured biomaterials based on calcium aluminate" in Srpski arhiv za celokupno lekarstvo, 146, no. 11-12 (2018):634-640,
https://doi.org/10.2298/SARH171211030J . .
1
1

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Jokanović, Vukoman; Čolović, Božana; Prokić, Bogomir Bolka; Tomanović, Nada; Popović-Bajić, Marijana; Živković, Slavoljub

(Hindawi Ltd, London, 2018)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Prokić, Bogomir Bolka
AU  - Tomanović, Nada
AU  - Popović-Bajić, Marijana
AU  - Živković, Slavoljub
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2313
AB  - As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.
PB  - Hindawi Ltd, London
T2  - Advances in Materials Science & Engineering
T1  - Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates
VL  - 2018
DO  - 10.1155/2018/8493439
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Prokić, Bogomir Bolka and Tomanović, Nada and Popović-Bajić, Marijana and Živković, Slavoljub",
year = "2018",
abstract = "As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in many in vitro and in vivo studies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.",
publisher = "Hindawi Ltd, London",
journal = "Advances in Materials Science & Engineering",
title = "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates",
volume = "2018",
doi = "10.1155/2018/8493439"
}
Jokanović, V., Čolović, B., Prokić, B. B., Tomanović, N., Popović-Bajić, M.,& Živković, S.. (2018). Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering
Hindawi Ltd, London., 2018.
https://doi.org/10.1155/2018/8493439
Jokanović V, Čolović B, Prokić BB, Tomanović N, Popović-Bajić M, Živković S. Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates. in Advances in Materials Science & Engineering. 2018;2018.
doi:10.1155/2018/8493439 .
Jokanović, Vukoman, Čolović, Božana, Prokić, Bogomir Bolka, Tomanović, Nada, Popović-Bajić, Marijana, Živković, Slavoljub, "Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates" in Advances in Materials Science & Engineering, 2018 (2018),
https://doi.org/10.1155/2018/8493439 . .
3
2
2

Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study

Ćetenović, Bojana; Čolović, Božana; Vasilijić, Saša; Prokić, Bogomir; Pašalić, Snežana; Jokanović, Vukoman; Tepavčević, Zvezdana; Marković, Dejan

(Springer, Dordrecht, 2018)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana
AU  - Vasilijić, Saša
AU  - Prokić, Bogomir
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman
AU  - Tepavčević, Zvezdana
AU  - Marković, Dejan
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2275
AB  - The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Journal of Materials Science-Materials in Medicine
T1  - Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study
VL  - 29
IS  - 12
DO  - 10.1007/s10856-018-6200-z
ER  - 
@article{
author = "Ćetenović, Bojana and Čolović, Božana and Vasilijić, Saša and Prokić, Bogomir and Pašalić, Snežana and Jokanović, Vukoman and Tepavčević, Zvezdana and Marković, Dejan",
year = "2018",
abstract = "The aim of this study was to investigate the biocompatibility of nanostructured materials based on highly active calcium silicates mixed with different radiocontrast agents in comparison to MTA(+) using in vitro and in vivo model. Morphology of materials' samples was analyzed using SEM while the phase compositions were identified by XRD. pH values of materials' suspensions were conducted by pH-meter. The cytotoxicity of materials' solutions was tested by MTT test (100, 50, 25 and 12.5mg/ml). LDH and H-3-thymidine assay were utilized for biocompatibility investigations of materials' eluates (24h, 7 day and 21 day). Eighteen Guinea pigs were used for intramuscular implantation, as teflon tubes with freshly prepared materials were placed into intramuscular pockets. All samples were composed of round and needle-like particles equally distributed with Ca/Si ratio 2.7 at%, with the presence of hydrated calcium silicate phases. The pH values of ALBO-MPCA(1) and ALBO-MPCA(2) were high alkaline, while in case of MTA(+) they were lower and continuously declined (p lt 0.05). Investigated materials didn't exhibit dose-dependent effect on metabolic activity of L929 cells (p>0.05). Significant differences in the percentage of cytotoxicity between diluted and undiluted extracts between all tested materials after 24h and 7 day were noticed (p lt 0.05). Increase in L929 cells proliferation was noticed in case of undiluted eluates of ALBO-MPCA(1) and ALBO-MPCA(2) after 7 day (p lt 0.05). There were no statistically significant differences in the intensity of inflammatory response between investigated materials and control group after 60 day (p>0.05). Evaluation of biocompatibility of both ALBO-MPCA(1) and ALBO-MPCA(2) indicate their potential clinical use. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Journal of Materials Science-Materials in Medicine",
title = "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study",
volume = "29",
number = "12",
doi = "10.1007/s10856-018-6200-z"
}
Ćetenović, B., Čolović, B., Vasilijić, S., Prokić, B., Pašalić, S., Jokanović, V., Tepavčević, Z.,& Marković, D.. (2018). Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine
Springer, Dordrecht., 29(12).
https://doi.org/10.1007/s10856-018-6200-z
Ćetenović B, Čolović B, Vasilijić S, Prokić B, Pašalić S, Jokanović V, Tepavčević Z, Marković D. Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study. in Journal of Materials Science-Materials in Medicine. 2018;29(12).
doi:10.1007/s10856-018-6200-z .
Ćetenović, Bojana, Čolović, Božana, Vasilijić, Saša, Prokić, Bogomir, Pašalić, Snežana, Jokanović, Vukoman, Tepavčević, Zvezdana, Marković, Dejan, "Nanostructured endodontic materials mixed with different radiocontrast agentsbiocompatibility study" in Journal of Materials Science-Materials in Medicine, 29, no. 12 (2018),
https://doi.org/10.1007/s10856-018-6200-z . .
4
4
6

Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers

Ćetenović, Bojana; Prokić, Bogomir; Vasilijić, Saša; Dojčinović, Biljana; Magić, Marko; Jokanović, Vukoman; Marković, Dejan

(Elsevier Science Inc, New York, 2017)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Prokić, Bogomir
AU  - Vasilijić, Saša
AU  - Dojčinović, Biljana
AU  - Magić, Marko
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2196
AB  - Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.
PB  - Elsevier Science Inc, New York
T2  - Journal of Endodontics
T1  - Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers
VL  - 43
IS  - 3
SP  - 425
EP  - 432
DO  - 10.1016/j.joen.2016.10.041
ER  - 
@article{
author = "Ćetenović, Bojana and Prokić, Bogomir and Vasilijić, Saša and Dojčinović, Biljana and Magić, Marko and Jokanović, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Introduction: The aim of this article was to analyze biocompatibility and bioactivity of new endodontic materials on the basis of nanosynthesized calcium silicates (ALBO-MPCA(1) and ALBO-MPCA(2)) combined with different radiopacifiers in comparison with MTA(+). Methods: Morphology of the samples was studied by scanning electron microscopy, and the pH and ion release analysis were also assessed. Biocompatibility of materials' eluates (24-hour, 7-day, and 21-day) was conducted by using MIT test. Twelve New Zealand white rabbits were used for intraosseous implantation. Four calvarial defects per animal were created and filled with freshly prepared investigated materials. Results: Samples mostly consisted of agglomerates built up from nanoparticles, preferably spherical and rod-like. There was no significant difference among pH values of materials' eluates after 24 hours (P>.05). The amount of calcium and aluminum ion release decreased, whereas the amount of magnesium and bismuth (ALBO-MPCAl, MTA(+)) and barium (ALBO-MPCA(2)) increased during 21-day period. The metabolic activity of cells increased after the extraction time, except in case of undiluted elutes of ALBO-MPCA(2) and ALBO-MPCAI (21-day). Histologic analysis of the samples revealed newly formed bone tissue with moderate inflammation for all investigated materials, which subsided during 90-day period to mild. Both MIA(+) and ALBO-MPCAI were in direct contact with the newly formed bone tissue. After 90 days, statistically significant difference in hard tissue formation was observed in comparison of MIA(+) and ALBO-MPCki with control group (P  lt .05). Conclusions: Experimental materials ALBOMPCA, and ALBO-MPCA(2) possess both biocompatibility and bioactivity. Because ALBO-MPCAA provokes favorable biological response, it is especially good candidate for further clinical investigations.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Endodontics",
title = "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers",
volume = "43",
number = "3",
pages = "425-432",
doi = "10.1016/j.joen.2016.10.041"
}
Ćetenović, B., Prokić, B., Vasilijić, S., Dojčinović, B., Magić, M., Jokanović, V.,& Marković, D.. (2017). Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics
Elsevier Science Inc, New York., 43(3), 425-432.
https://doi.org/10.1016/j.joen.2016.10.041
Ćetenović B, Prokić B, Vasilijić S, Dojčinović B, Magić M, Jokanović V, Marković D. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers. in Journal of Endodontics. 2017;43(3):425-432.
doi:10.1016/j.joen.2016.10.041 .
Ćetenović, Bojana, Prokić, Bogomir, Vasilijić, Saša, Dojčinović, Biljana, Magić, Marko, Jokanović, Vukoman, Marković, Dejan, "Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Combined with Different Radiopacifiers" in Journal of Endodontics, 43, no. 3 (2017):425-432,
https://doi.org/10.1016/j.joen.2016.10.041 . .
3
9
9
10

Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation

Jokanović, Vukoman; Čolović, Božana; Marković, Dejan; Petrović, Milan; Soldatović, Ivan; Antonijević, Đorđe; Milosavljević, Petar; Sjerobabin, Nikola; Sopta, Jelena

(Walter De Gruyter Gmbh, Berlin, 2017)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Soldatović, Ivan
AU  - Antonijević, Đorđe
AU  - Milosavljević, Petar
AU  - Sjerobabin, Nikola
AU  - Sopta, Jelena
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2187
AB  - This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.
PB  - Walter De Gruyter Gmbh, Berlin
T2  - Biomedical Engineering - Biomedizinische Technik
T1  - Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation
VL  - 62
IS  - 3
SP  - 295
EP  - 306
DO  - 10.1515/bmt-2015-0164
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Soldatović, Ivan and Antonijević, Đorđe and Milosavljević, Petar and Sjerobabin, Nikola and Sopta, Jelena",
year = "2017",
abstract = "This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffold's suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.",
publisher = "Walter De Gruyter Gmbh, Berlin",
journal = "Biomedical Engineering - Biomedizinische Technik",
title = "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation",
volume = "62",
number = "3",
pages = "295-306",
doi = "10.1515/bmt-2015-0164"
}
Jokanović, V., Čolović, B., Marković, D., Petrović, M., Soldatović, I., Antonijević, Đ., Milosavljević, P., Sjerobabin, N.,& Sopta, J.. (2017). Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik
Walter De Gruyter Gmbh, Berlin., 62(3), 295-306.
https://doi.org/10.1515/bmt-2015-0164
Jokanović V, Čolović B, Marković D, Petrović M, Soldatović I, Antonijević Đ, Milosavljević P, Sjerobabin N, Sopta J. Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering - Biomedizinische Technik. 2017;62(3):295-306.
doi:10.1515/bmt-2015-0164 .
Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Soldatović, Ivan, Antonijević, Đorđe, Milosavljević, Petar, Sjerobabin, Nikola, Sopta, Jelena, "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation" in Biomedical Engineering - Biomedizinische Technik, 62, no. 3 (2017):295-306,
https://doi.org/10.1515/bmt-2015-0164 . .
1
10
7
9

Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study

Opačić-Galić, Vanja; Petrović, Violeta; Jokanović, Vukoman; Živković, Slavoljub

(Srpsko lekarsko društvo, Beograd, 2017)

TY  - JOUR
AU  - Opačić-Galić, Vanja
AU  - Petrović, Violeta
AU  - Jokanović, Vukoman
AU  - Živković, Slavoljub
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2261
AB  - Introduction/Objective: Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatite-based (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods: The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results: Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ± 0.48), while CS and CS-HA scored 3 ± 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ± 0.45). The least visible inflammatory reaction of the rabbits’ pulp tissue was spotted with the CS (1.83 ± 0.75), than with the MTA and CS-HA (2.67 ± 1.53, 3 ± 0.63). Conclusion: The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits’ pulp tissue only in the immediate vicinity of the implanted material.
AB  - Uvod/Cilj: Usavršavanje materijala koji bi mogli da se koriste kao biološke zamene kosti jedna je od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Cilj ovog rada je bio da se ispita odgovor tkiva na novosintetisane nanomaterijale na bazi kalcijum-silikatnih sistema (KS) i hidroksiapatita (KS-HA) posle implantacije u potkožno tkivo pacova i direktnog prekrivanja pulpe zuba kunića. Metode: U potkožno tkivo 40 vistar pacova su implantirani testirani materijali, a posle 7, 15, 30 i 60 dana životinje su žrtvovane. Direktno prekrivanje pulpe je realizovano na zubima kunića. Na vestibularnim površinama sekutića preparisani su kaviteti, a eksponirana pulpa je prekrivana testiranim materijalima. Životinje su žrtvovane posle 10 i 15 dana. Kontrolni materijal u oba eksperimenta je bio mineralni trioksidni agregat (MTA). Histološka analiza je obuhvatila praćenje ćelijske komponente zapaljenja, prisustva gigantskih ćelija i nekroze tkiva. Rezultati: Sedam dana posle supkutane implantacije najjači zapaljenski odgovor dao je MTA (3,30 ± 0,48), dok je za KS i KS-HA on ocenjen sa 3,00 ± 0,71. Posle 60 dana došlo je do opadanja znakova zapaljenja, koje je bilo najmanje izraženo oko KS-HA (0,20 ± 0,45). Najmanje izražena zapaljenska reakcija pulpnog tkiva kunića uočena je kod materijala KS (1,83 ± 0,75), zatim kod MTA i KS-HA (2,67 ± 1,53, 3,00 ± 0,63). Zaključak: Novosintetisani materijali su izazvali blagu zapaljensku reakciju potkožnog tkiva pacova, a KS-HA je pokazao najbolju tkivnu toleranciju. Nanostrukturni biomaterijali KS i KS-HA su uzrokovali blagu do umerenu zapaljensku reakciju pupnog tkiva kunića samo u neposrednoj blizini implantiranog materijala.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study
T1  - Histološke reakcije tkiva na novosintetisane bioaktivne materijale na bazi kalcijum-silikatnih sistema i hidroksiapatita – In vivo studija
VL  - 145
IS  - 7-8
SP  - 370
EP  - 377
DO  - 10.2298/SARH160719063O
ER  - 
@article{
author = "Opačić-Galić, Vanja and Petrović, Violeta and Jokanović, Vukoman and Živković, Slavoljub",
year = "2017",
abstract = "Introduction/Objective: Development of materials which could be used as biological bone substitutes is one of the most valuable and active fields of biomaterial research. The goal of the study was to research the reaction of tissue on calcium silicate- (CS) and hydroxyapatite-based (CS-HA) newly synthesized nanomaterials, after being implanted into the subcutaneous tissue of a rats and direct pulp capping of rabbit teeth. Methods: The tested materials were implanted in 40 Wistar male rats, sacrificed after seven, 15, 30, and 60 days. The direct pulp capping was performed on the teeth of rabbits. Cavities were prepared on the vestibular surface of the incisors. The animals were sacrificed after 10 and 15 days. The control material was mineral trioxide aggregate (MTA). Histological analysis covered the tracking of inflammatory reaction cellular components, presence of gigantic cells, and necrosis of the tissue. Results: Seven days after the implantation, the strongest inflammatory response was given by the MTA (3.3 ± 0.48), while CS and CS-HA scored 3 ± 0.71. After 60 days, the rate of inflammatory reactions dropped, which was the least visible with CS-HA (0.2 ± 0.45). The least visible inflammatory reaction of the rabbits’ pulp tissue was spotted with the CS (1.83 ± 0.75), than with the MTA and CS-HA (2.67 ± 1.53, 3 ± 0.63). Conclusion: The newly synthesized materials caused a slight reaction of the subcutaneous tissue. CS-HA showed the best tissue tolerance. Nanostructural biomaterials caused a slight to moderate inflammatory reaction of the rabbits’ pulp tissue only in the immediate vicinity of the implanted material., Uvod/Cilj: Usavršavanje materijala koji bi mogli da se koriste kao biološke zamene kosti jedna je od najznačajnijih i najaktivnijih oblasti istraživanja biomaterijala. Cilj ovog rada je bio da se ispita odgovor tkiva na novosintetisane nanomaterijale na bazi kalcijum-silikatnih sistema (KS) i hidroksiapatita (KS-HA) posle implantacije u potkožno tkivo pacova i direktnog prekrivanja pulpe zuba kunića. Metode: U potkožno tkivo 40 vistar pacova su implantirani testirani materijali, a posle 7, 15, 30 i 60 dana životinje su žrtvovane. Direktno prekrivanje pulpe je realizovano na zubima kunića. Na vestibularnim površinama sekutića preparisani su kaviteti, a eksponirana pulpa je prekrivana testiranim materijalima. Životinje su žrtvovane posle 10 i 15 dana. Kontrolni materijal u oba eksperimenta je bio mineralni trioksidni agregat (MTA). Histološka analiza je obuhvatila praćenje ćelijske komponente zapaljenja, prisustva gigantskih ćelija i nekroze tkiva. Rezultati: Sedam dana posle supkutane implantacije najjači zapaljenski odgovor dao je MTA (3,30 ± 0,48), dok je za KS i KS-HA on ocenjen sa 3,00 ± 0,71. Posle 60 dana došlo je do opadanja znakova zapaljenja, koje je bilo najmanje izraženo oko KS-HA (0,20 ± 0,45). Najmanje izražena zapaljenska reakcija pulpnog tkiva kunića uočena je kod materijala KS (1,83 ± 0,75), zatim kod MTA i KS-HA (2,67 ± 1,53, 3,00 ± 0,63). Zaključak: Novosintetisani materijali su izazvali blagu zapaljensku reakciju potkožnog tkiva pacova, a KS-HA je pokazao najbolju tkivnu toleranciju. Nanostrukturni biomaterijali KS i KS-HA su uzrokovali blagu do umerenu zapaljensku reakciju pupnog tkiva kunića samo u neposrednoj blizini implantiranog materijala.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study, Histološke reakcije tkiva na novosintetisane bioaktivne materijale na bazi kalcijum-silikatnih sistema i hidroksiapatita – In vivo studija",
volume = "145",
number = "7-8",
pages = "370-377",
doi = "10.2298/SARH160719063O"
}
Opačić-Galić, V., Petrović, V., Jokanović, V.,& Živković, S.. (2017). Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 145(7-8), 370-377.
https://doi.org/10.2298/SARH160719063O
Opačić-Galić V, Petrović V, Jokanović V, Živković S. Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study. in Srpski arhiv za celokupno lekarstvo. 2017;145(7-8):370-377.
doi:10.2298/SARH160719063O .
Opačić-Galić, Vanja, Petrović, Violeta, Jokanović, Vukoman, Živković, Slavoljub, "Histological evaluation of tissue reactions to newly synthetized calcium silicate-and hydroxyapatite-based bioactive materials: In vivo study" in Srpski arhiv za celokupno lekarstvo, 145, no. 7-8 (2017):370-377,
https://doi.org/10.2298/SARH160719063O . .
6
4
5

Scaffold in bone tissue engineering

Jokanović, Vukoman; Čolović, Božana; Popović-Bajić, Marijana; Živković-Sandić, Marija

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2017)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Popović-Bajić, Marijana
AU  - Živković-Sandić, Marija
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2252
AB  - Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods.
AB  - Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Scaffold in bone tissue engineering
T1  - Skafoldi u inženjerstvu koštanog tkiva
VL  - 64
IS  - 1
SP  - 32
EP  - 40
DO  - 10.1515/sdj-2017-0004
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Popović-Bajić, Marijana and Živković-Sandić, Marija",
year = "2017",
abstract = "Treatment of bone tissue injuries and diseases is still a great challenge for surgeons, but also for researchers who work with materials. Today stem cells are commonly used in bone tissue engineering. However, advances in biocompatible materials design, especially biodegradable porous structure (scaffold) is gaining an important role in the treatment of diseased bone tissue. The basic advantage of these carriers is specifically designed scaffold with defined porosity and pore structure that is favourable for cells settlement. Scaffolds are most commonly used as ceramic brackets because they have excellent characteristics in biodegradation and bioactivity. The process of scaffold production is important because the appropriate technology must ensure control of liquids and reproducibility of scaffold production through standardized process. The aim of this study was to present some of different procedures of scaffold production in bone tissue engineering and point out the advantages and disadvantages of these methods., Terapija i lečenje brojnih povreda i oboljenja koštanog tkiva je još uvek veliki izazov za hirurge, ali i za one istraživače koji se bave materijalima. Na polju inženjerstva koštanog tkiva danas se najčešće koriste matične ćelije. Međutim, napredak u dizajniranju biokompatibilnih materijala, a posebno biodegradibilnih poroznih struktura (skafolda) sve više dobija vrlo značajnu ulogu u lečenju obolelih koštanih tkiva. Specifično dizajnirani skafoldi sa definisanom poroznošću i strukturom pora koja je povoljna za naseljavanje ćelija osnovna je prednost ovih nosača. Skafoldi se najčešće koriste kao keramički nosači jer imaju izvanredne osobine vezane za biodegradaciju i jako izraženu bioaktivnost. Postupak izrade skafolda je vrlo važan jer se odgovarajućom tehnologijom mora obezbediti kontrola tečnosti i reproduktivnost izrade skafolda kroz standardizaciju procesa. Cilj ovog rada je bio da se predstave različiti metodološki postupci izrade skafolda u inženjerstvu koštanog tkiva i ukaže na određene prednosti i nedostatke tih metoda.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Scaffold in bone tissue engineering, Skafoldi u inženjerstvu koštanog tkiva",
volume = "64",
number = "1",
pages = "32-40",
doi = "10.1515/sdj-2017-0004"
}
Jokanović, V., Čolović, B., Popović-Bajić, M.,& Živković-Sandić, M.. (2017). Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 64(1), 32-40.
https://doi.org/10.1515/sdj-2017-0004
Jokanović V, Čolović B, Popović-Bajić M, Živković-Sandić M. Scaffold in bone tissue engineering. in Stomatološki glasnik Srbije. 2017;64(1):32-40.
doi:10.1515/sdj-2017-0004 .
Jokanović, Vukoman, Čolović, Božana, Popović-Bajić, Marijana, Živković-Sandić, Marija, "Scaffold in bone tissue engineering" in Stomatološki glasnik Srbije, 64, no. 1 (2017):32-40,
https://doi.org/10.1515/sdj-2017-0004 . .
2

In vitro biocompatibility of nanostructured endodontic materials using SCAP cells

Ćetenović, Bojana; Čolović, Božana; Vasilijić, Saša; Pašalić, Snežana; Jokanović, Vukoman; Marković, Dejan

(Udruženje stomatologa Balkana, 2017)

TY  - JOUR
AU  - Ćetenović, Bojana
AU  - Čolović, Božana
AU  - Vasilijić, Saša
AU  - Pašalić, Snežana
AU  - Jokanović, Vukoman
AU  - Marković, Dejan
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2249
AB  - Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes after the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for further clinical studies.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Dental Medicine
T1  - In vitro biocompatibility of nanostructured endodontic materials using SCAP cells
VL  - 21
IS  - 3
SP  - 167
EP  - 170
DO  - 10.1515/bjdm-2017-0029
ER  - 
@article{
author = "Ćetenović, Bojana and Čolović, Božana and Vasilijić, Saša and Pašalić, Snežana and Jokanović, Vukoman and Marković, Dejan",
year = "2017",
abstract = "Background/Aim: Lately, fully innovative sol-gel method with high-temperature self-propagating reaction was used for the synthesis of new nanostructured endodontic materials, in combination with different radiopacifiers: bismuth (ALBO-MPCA1) and barium (ALBO-MPCA2). The aim of this study was to investigate the biocompatibility of nanostructured endodontic materials based on highly active calcium silicates and mixed with different radiopacifiers in comparison to MTA+ using human stem cells from the apical papilla- SCAP cells. Material and Methods: Morphology of the samples was studied by SEM. The tested materials were mixed with distilled water in a ratio 2:1 (m/m). Fifteen minutes after the preparation, samples were used in the experiment. The biocompatibility of fresh materials, after 3h and 7 days, was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide- MTT test. Results: Samples mostly consisted of spherical and rode-like. The relative viability of cells increased following the exposure time. Conclusion: The biocompatibility of synthesized materials is comparable to the control material MTA+, and therefore these materials can be recommended for further clinical studies.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Dental Medicine",
title = "In vitro biocompatibility of nanostructured endodontic materials using SCAP cells",
volume = "21",
number = "3",
pages = "167-170",
doi = "10.1515/bjdm-2017-0029"
}
Ćetenović, B., Čolović, B., Vasilijić, S., Pašalić, S., Jokanović, V.,& Marković, D.. (2017). In vitro biocompatibility of nanostructured endodontic materials using SCAP cells. in Balkan Journal of Dental Medicine
Udruženje stomatologa Balkana., 21(3), 167-170.
https://doi.org/10.1515/bjdm-2017-0029
Ćetenović B, Čolović B, Vasilijić S, Pašalić S, Jokanović V, Marković D. In vitro biocompatibility of nanostructured endodontic materials using SCAP cells. in Balkan Journal of Dental Medicine. 2017;21(3):167-170.
doi:10.1515/bjdm-2017-0029 .
Ćetenović, Bojana, Čolović, Božana, Vasilijić, Saša, Pašalić, Snežana, Jokanović, Vukoman, Marković, Dejan, "In vitro biocompatibility of nanostructured endodontic materials using SCAP cells" in Balkan Journal of Dental Medicine, 21, no. 3 (2017):167-170,
https://doi.org/10.1515/bjdm-2017-0029 . .
2

Various methods of 3D and bio-printing

Jokanović, Vukoman; Čolović, Božana; Antonijević, Đorđe; Mićić, Milutin; Živković, Slavoljub

(Srpsko lekarsko društvo - Stomatološka sekcija, Beograd, 2017)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Antonijević, Đorđe
AU  - Mićić, Milutin
AU  - Živković, Slavoljub
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2242
AB  - There is growing need for synthetic tissue replacement materials designed in a way that mimic complex structure of tissues and organs. Among various methods for fabrication of implants (scaffolds), 3D printing is very powerful technique because it enables creation of scaffolds with complex internal structures and high resolution, based on medical data sets. This method allows fabrication of scaffolds with desired macro- and micro-porosity and fully inter- connected pore network. Rapid development of 3D printing technologies has enabled various applications from the creation of anatomical training models for complex surgical procedures to the printing of tissue engineering constructs. The aim of current investigations was to develop compatible printers and materials (bioinks) to obtain biomimetic scaffolds, which allow printing of living cells without significant loss of cell viability. The advanced level of such printing assumes “in situ” printing, i.e. printing cells and biomaterials directly onto or in a patient that will reduce recovery time.
AB  - Danas postoji sve veća potreba za sintetičkim materijalima za zamenu tkiva dizajniranih na način koji imitira složenu strukturu tkiva i organa. Među različitim metodama proizvodnje implantata (skafolda), 3D štampanje je veoma moćna tehnika jer omogućava kreiranje skafolda sa složenim unutrašnjim strukturama i visokom rezolucijom, zasnovanim na medicinskim skupovima podataka. Ova metoda omogućava proizvodnju skafolda sa željenom makroporoznošću i mikroporoznošću i potpuno povezanom mrežom pora. Brzi razvoj tehnologija 3D štampanja omogućio je različite primene – od kreiranja anatomskih modela za uvežbavanje složenih hirurških procedura do štampanja konstrukata za tkivno inženjerstvo. Cilj tekućih istraživanja je razvoj kompatibilnih štampača i materijala (bio-mastila) za dobijanje biomimičnih skafolda, koji omogućavaju štampanje živih ćelija bez značajnog gubitka njihove vijabilnosti. Napredni nivo takvog štampanja pretpostavlja štampanje in situ, tj. štampanje ćelija i biomaterijala direktno na pacijentu ili u pacijenta, što će smanjiti vreme oporavka.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Various methods of 3D and bio-printing
T1  - Različite metode 3D štampanja i bio-štampanja
VL  - 64
IS  - 3
SP  - 136
EP  - 145
DO  - 10.1515/sdj-2017-0014
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Antonijević, Đorđe and Mićić, Milutin and Živković, Slavoljub",
year = "2017",
abstract = "There is growing need for synthetic tissue replacement materials designed in a way that mimic complex structure of tissues and organs. Among various methods for fabrication of implants (scaffolds), 3D printing is very powerful technique because it enables creation of scaffolds with complex internal structures and high resolution, based on medical data sets. This method allows fabrication of scaffolds with desired macro- and micro-porosity and fully inter- connected pore network. Rapid development of 3D printing technologies has enabled various applications from the creation of anatomical training models for complex surgical procedures to the printing of tissue engineering constructs. The aim of current investigations was to develop compatible printers and materials (bioinks) to obtain biomimetic scaffolds, which allow printing of living cells without significant loss of cell viability. The advanced level of such printing assumes “in situ” printing, i.e. printing cells and biomaterials directly onto or in a patient that will reduce recovery time., Danas postoji sve veća potreba za sintetičkim materijalima za zamenu tkiva dizajniranih na način koji imitira složenu strukturu tkiva i organa. Među različitim metodama proizvodnje implantata (skafolda), 3D štampanje je veoma moćna tehnika jer omogućava kreiranje skafolda sa složenim unutrašnjim strukturama i visokom rezolucijom, zasnovanim na medicinskim skupovima podataka. Ova metoda omogućava proizvodnju skafolda sa željenom makroporoznošću i mikroporoznošću i potpuno povezanom mrežom pora. Brzi razvoj tehnologija 3D štampanja omogućio je različite primene – od kreiranja anatomskih modela za uvežbavanje složenih hirurških procedura do štampanja konstrukata za tkivno inženjerstvo. Cilj tekućih istraživanja je razvoj kompatibilnih štampača i materijala (bio-mastila) za dobijanje biomimičnih skafolda, koji omogućavaju štampanje živih ćelija bez značajnog gubitka njihove vijabilnosti. Napredni nivo takvog štampanja pretpostavlja štampanje in situ, tj. štampanje ćelija i biomaterijala direktno na pacijentu ili u pacijenta, što će smanjiti vreme oporavka.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Various methods of 3D and bio-printing, Različite metode 3D štampanja i bio-štampanja",
volume = "64",
number = "3",
pages = "136-145",
doi = "10.1515/sdj-2017-0014"
}
Jokanović, V., Čolović, B., Antonijević, Đ., Mićić, M.,& Živković, S.. (2017). Various methods of 3D and bio-printing. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 64(3), 136-145.
https://doi.org/10.1515/sdj-2017-0014
Jokanović V, Čolović B, Antonijević Đ, Mićić M, Živković S. Various methods of 3D and bio-printing. in Stomatološki glasnik Srbije. 2017;64(3):136-145.
doi:10.1515/sdj-2017-0014 .
Jokanović, Vukoman, Čolović, Božana, Antonijević, Đorđe, Mićić, Milutin, Živković, Slavoljub, "Various methods of 3D and bio-printing" in Stomatološki glasnik Srbije, 64, no. 3 (2017):136-145,
https://doi.org/10.1515/sdj-2017-0014 . .
1

Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth

Marković, Dejan; Ćetenović, Bojana; Vuković, Ana; Jokanović, Vukoman; Marković, Tatjana

(Elsevier Inc., 2016)

TY  - CHAP
AU  - Marković, Dejan
AU  - Ćetenović, Bojana
AU  - Vuković, Ana
AU  - Jokanović, Vukoman
AU  - Marković, Tatjana
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2154
AB  - Calcium-silicate-based materials are used in many endodontic and oral-surgical procedures. The advantages of calcium-silicate-based materials are good apical sealing, setting in the presence of moisture, and acquiring high pH after mixing. However, these materials also have some disadvantages: low flowability, dry consistency, and long setting time. By applying nanotechnology during synthesis, very active calcium silicate, such as nanostructured mineral polyoxide carbonate aggregate, may be used to overcome the above-mentioned disadvantages and further enhance physical properties of the reaction mixture consisting of calcite and monoclinic bismuth oxide. Application of the sol-gel method for calcite production and combination of such a method with high-temperature, self-propagating synthesis of calcium silicates makes the strategy completely innovative. Materials based on nanostructured calcium silicates may represent effective therapeutic agents for root canal obturation, especially in the case of immature roots. The application of calcium-silicate-based materials may significantly decrease the duration of therapy, reduce the risk of tooth fractures, and overcome incomplete calcification problem.
PB  - Elsevier Inc.
T2  - Nanobiomaterials in Dentistry: Applications of Nanobiomaterials
T1  - Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth
VL  - 11
SP  - 269
EP  - 307
DO  - 10.1016/B978-0-323-42867-5.00011-4
ER  - 
@inbook{
author = "Marković, Dejan and Ćetenović, Bojana and Vuković, Ana and Jokanović, Vukoman and Marković, Tatjana",
year = "2016",
abstract = "Calcium-silicate-based materials are used in many endodontic and oral-surgical procedures. The advantages of calcium-silicate-based materials are good apical sealing, setting in the presence of moisture, and acquiring high pH after mixing. However, these materials also have some disadvantages: low flowability, dry consistency, and long setting time. By applying nanotechnology during synthesis, very active calcium silicate, such as nanostructured mineral polyoxide carbonate aggregate, may be used to overcome the above-mentioned disadvantages and further enhance physical properties of the reaction mixture consisting of calcite and monoclinic bismuth oxide. Application of the sol-gel method for calcite production and combination of such a method with high-temperature, self-propagating synthesis of calcium silicates makes the strategy completely innovative. Materials based on nanostructured calcium silicates may represent effective therapeutic agents for root canal obturation, especially in the case of immature roots. The application of calcium-silicate-based materials may significantly decrease the duration of therapy, reduce the risk of tooth fractures, and overcome incomplete calcification problem.",
publisher = "Elsevier Inc.",
journal = "Nanobiomaterials in Dentistry: Applications of Nanobiomaterials",
booktitle = "Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth",
volume = "11",
pages = "269-307",
doi = "10.1016/B978-0-323-42867-5.00011-4"
}
Marković, D., Ćetenović, B., Vuković, A., Jokanović, V.,& Marković, T.. (2016). Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth. in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials
Elsevier Inc.., 11, 269-307.
https://doi.org/10.1016/B978-0-323-42867-5.00011-4
Marković D, Ćetenović B, Vuković A, Jokanović V, Marković T. Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth. in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials. 2016;11:269-307.
doi:10.1016/B978-0-323-42867-5.00011-4 .
Marković, Dejan, Ćetenović, Bojana, Vuković, Ana, Jokanović, Vukoman, Marković, Tatjana, "Nanosynthesized calcium-silicate-based biomaterials in endodontic treatment of young permanent teeth" in Nanobiomaterials in Dentistry: Applications of Nanobiomaterials, 11 (2016):269-307,
https://doi.org/10.1016/B978-0-323-42867-5.00011-4 . .
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7

Biological aspects of application of nanomaterials in tissue engineering

Marković, Dejan; Karadžić, Ivana; Jokanović, Vukoman; Vuković, Ana; Vučić, Vesna

(Savez hemijskih inženjera, Beograd, 2016)

TY  - JOUR
AU  - Marković, Dejan
AU  - Karadžić, Ivana
AU  - Jokanović, Vukoman
AU  - Vuković, Ana
AU  - Vučić, Vesna
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2083
AB  - Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly / CICEQ
T1  - Biological aspects of application of nanomaterials in tissue engineering
VL  - 22
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2298/CICEQ141231028M
ER  - 
@article{
author = "Marković, Dejan and Karadžić, Ivana and Jokanović, Vukoman and Vuković, Ana and Vučić, Vesna",
year = "2016",
abstract = "Millions of patients worldwide need surgery to repair or replace tissue that has been damaged through trauma or disease. To solve the problem of lost tissue, a major emphasis of tissue engineering (TE) is on tissue regeneration. Stem cells and highly porous biomaterials used as cell carriers (scaffolds) have an essential role in the production of new tissue by TE. The cellular component is important for the generation and establishment of the extracellular matrix, while a scaffold is necessary to determine the shape of the newly formed tissue and facilitate migration of cells into the desired location, as well as their growth and differentiation. This review describes the types, characteristics and classification of stem cells. Furthermore, it includes functional features of cell carriers - biocompatibility, biodegradability and mechanical properties of biomaterials used in developing state-of-the-an scaffolds for TE applications, as well as suitability for different tissues. Moreover, it explains the importance of nanotechnology and defines the challenges and the purpose of future research in this rapidly advancing field.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly / CICEQ",
title = "Biological aspects of application of nanomaterials in tissue engineering",
volume = "22",
number = "2",
pages = "145-153",
doi = "10.2298/CICEQ141231028M"
}
Marković, D., Karadžić, I., Jokanović, V., Vuković, A.,& Vučić, V.. (2016). Biological aspects of application of nanomaterials in tissue engineering. in Chemical Industry & Chemical Engineering Quarterly / CICEQ
Savez hemijskih inženjera, Beograd., 22(2), 145-153.
https://doi.org/10.2298/CICEQ141231028M
Marković D, Karadžić I, Jokanović V, Vuković A, Vučić V. Biological aspects of application of nanomaterials in tissue engineering. in Chemical Industry & Chemical Engineering Quarterly / CICEQ. 2016;22(2):145-153.
doi:10.2298/CICEQ141231028M .
Marković, Dejan, Karadžić, Ivana, Jokanović, Vukoman, Vuković, Ana, Vučić, Vesna, "Biological aspects of application of nanomaterials in tissue engineering" in Chemical Industry & Chemical Engineering Quarterly / CICEQ, 22, no. 2 (2016):145-153,
https://doi.org/10.2298/CICEQ141231028M . .
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Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design

Sjerobabin, Nikola; Čolović, Božana; Petrović, Milan; Marković, Dejan; Živković, Slavoljub; Jokanović, Vukoman

(Srpsko lekarsko društvo, Beograd, 2016)

TY  - JOUR
AU  - Sjerobabin, Nikola
AU  - Čolović, Božana
AU  - Petrović, Milan
AU  - Marković, Dejan
AU  - Živković, Slavoljub
AU  - Jokanović, Vukoman
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2162
AB  - Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained.
AB  - Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design
T1  - Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom
VL  - 144
IS  - 5-6
SP  - 280
EP  - 287
DO  - 10.2298/SARH1606280S
ER  - 
@article{
author = "Sjerobabin, Nikola and Čolović, Božana and Petrović, Milan and Marković, Dejan and Živković, Slavoljub and Jokanović, Vukoman",
year = "2016",
abstract = "Introduction: Biodegradable porous scaffolds are found to be very promising bone substitutes, acting as a temporary physical support to guide new tissue regeneration, until the entire scaffold is totally degraded and replaced by the new tissue. Objective The aim of this study was to investigate cytotoxicity of a synthesized calcium hydroxyapatite-based scaffold, named ALBO-OS, with high porosity and optimal topology. Methods The ALBO-OS scaffold was synthesized by the method of polymer foam template. The analysis of pore geometry and scaffold walls' topography was made by scanning electron microscope (SEM). The biological investigations assumed the examinations of ALBO-OS cytotoxicity to mouse L929 fibroblasts, using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) and lactate dehydrogenase (LDH) tests and inverse phase microscopy. Results The SEM analysis showed high porosity with fair pore distribution and interesting morphology from the biological standpoint. The biological investigations showed that the material is not cytotoxic to L929 cells. Comparison of ALBO-OS with Bio-Oss, as the global gold standard as a bone substitute, showed similar results in MTT test, while LDH test showed significantly higher rate of cell multiplication with ALBO-OS. Conclusion The scaffold design from the aspect of pore size, distribution, and topology seems to be very convenient for cell adhesion and occupation, which makes it a promising material as a bone substitute. The results of biological assays proved that ALBO-OS is not cytotoxic for L929 fibroblasts. In comparison with Bio-Oss, similar or even better results were obtained., Uvod: Porozni biodegradabilni nosači su se pokazali kao dobri zamenici kosti jer deluju kao privremena fizička potpora za usmerenu regeneraciju tkiva dok se čitav nosač potpuno ne razgradi i zameni novim tkivom. Cilj rada Cilj ovog rada je bio da se ispita citotoksičnost nosača tkiva na bazi kalcijum-hidroksiapatita, visoke poroznosti i optimalne topologije, nazvanog ALBO-OS. Metode rada Zamenik kosti ALBO-OS je sintetisan metodom matrice napravljene od polimerne pene. Geometrija pora i zidova nosača analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Biološka istraživanja izvedena su ispitivanjem citotoksičnosti ALBO-OS-a na mišjim fibroblastima L929 pomoću MTT i LDH testova i fazno kontrastne mikroskopije. Rezultati SEM analiza je pokazala veliku i ravnomernu poroznost i zanimljivu morfologiju sa biološkog stanovišta. Biološka istraživanja pokazala su da materijal nije citotoksičan. Poređenjem materijala ALBO-OS i Bio-Oss, koji je, globalno, zlatni standard među zamenicima kosti, dobijeni su slični rezultati na MTT testu, dok su rezulati LDH testa pokazali značajno veći broj ćelijskih deoba u kontaktu sa ALBO-OS-om. Zaključak Dizajn nosača sa stanovišta raspodele veličine pora i topologije je veoma pogodan za adheziju i naseljavanje ćelija, zbog čega ima veliki potencijal kao zamenik kosti. Rezultati bioloških testova su pokazali da ALBO-OS nije citotoksičan za L929 fibroblaste. U poređenju sa materijalom Bio-Oss, dobijeni su slični ili bolji rezultati.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design, Ispitivanje citotoksičnosti nosača na bazi hidroksiapatita s unapređenim strukturnim dizajnom",
volume = "144",
number = "5-6",
pages = "280-287",
doi = "10.2298/SARH1606280S"
}
Sjerobabin, N., Čolović, B., Petrović, M., Marković, D., Živković, S.,& Jokanović, V.. (2016). Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(5-6), 280-287.
https://doi.org/10.2298/SARH1606280S
Sjerobabin N, Čolović B, Petrović M, Marković D, Živković S, Jokanović V. Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):280-287.
doi:10.2298/SARH1606280S .
Sjerobabin, Nikola, Čolović, Božana, Petrović, Milan, Marković, Dejan, Živković, Slavoljub, Jokanović, Vukoman, "Cytotoxicity investigation of a new hydroxyapatite scaffold with improved structural design" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):280-287,
https://doi.org/10.2298/SARH1606280S . .
1
1
1

In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA

Jokanović, Vukoman; Čolović, Božana; Marković, Dejan; Petrović, Milan; Jokanović, Milan; Milosavljević, Petar; Sopta, Jelena

(Hindawi Ltd, London, 2016)

TY  - JOUR
AU  - Jokanović, Vukoman
AU  - Čolović, Božana
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Jokanović, Milan
AU  - Milosavljević, Petar
AU  - Sopta, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2168
AB  - A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.
PB  - Hindawi Ltd, London
T2  - Journal of Nanomaterials
T1  - In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA
VL  - 2016
DO  - 10.1155/2016/3948768
ER  - 
@article{
author = "Jokanović, Vukoman and Čolović, Božana and Marković, Dejan and Petrović, Milan and Jokanović, Milan and Milosavljević, Petar and Sopta, Jelena",
year = "2016",
abstract = "A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Nanomaterials",
title = "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA",
volume = "2016",
doi = "10.1155/2016/3948768"
}
Jokanović, V., Čolović, B., Marković, D., Petrović, M., Jokanović, M., Milosavljević, P.,& Sopta, J.. (2016). In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/3948768
Jokanović V, Čolović B, Marković D, Petrović M, Jokanović M, Milosavljević P, Sopta J. In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials. 2016;2016.
doi:10.1155/2016/3948768 .
Jokanović, Vukoman, Čolović, Božana, Marković, Dejan, Petrović, Milan, Jokanović, Milan, Milosavljević, Petar, Sopta, Jelena, "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA" in Journal of Nanomaterials, 2016 (2016),
https://doi.org/10.1155/2016/3948768 . .
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