TY  - JOUR
AU  - De Marco, Patrizia
AU  - Zara, Susi
AU  - De Colli, Marianna
AU  - Radunović, Milena
AU  - Lazović, Vladimir
AU  - Ettorre, Valeria
AU  - Di Crescenzo, Antonello
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Fontana, Antonella
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2218
AB  - Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.
PB  - Iop Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts
VL  - 12
IS  - 5
DO  - 10.1088/1748-605X/aa7907
ER  - 

@article{
author = "De Marco, Patrizia and Zara, Susi and De Colli, Marianna and Radunović, Milena and Lazović, Vladimir and Ettorre, Valeria and Di Crescenzo, Antonello and Piattelli, Adriano and Cataldi, Amelia and Fontana, Antonella",
year = "2017",
abstract = "Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts",
volume = "12",
number = "5",
doi = "10.1088/1748-605X/aa7907"
}

De Marco, P., Zara, S., De Colli, M., Radunović, M., Lazović, V., Ettorre, V., Di Crescenzo, A., Piattelli, A., Cataldi, A.,& Fontana, A.. (2017). Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials
Iop Publishing Ltd, Bristol., 12(5).
https://doi.org/10.1088/1748-605X/aa7907

De Marco P, Zara S, De Colli M, Radunović M, Lazović V, Ettorre V, Di Crescenzo A, Piattelli A, Cataldi A, Fontana A. Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials. 2017;12(5).
doi:10.1088/1748-605X/aa7907 .

De Marco, Patrizia, Zara, Susi, De Colli, Marianna, Radunović, Milena, Lazović, Vladimir, Ettorre, Valeria, Di Crescenzo, Antonello, Piattelli, Adriano, Cataldi, Amelia, Fontana, Antonella, "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts" in Biomedical Materials, 12, no. 5 (2017),
https://doi.org/10.1088/1748-605X/aa7907 . .

TY  - JOUR
AU  - Radunović, Milena
AU  - De Colli, Marianna
AU  - De Marco, Patrizia
AU  - Di Nisio, Chiara
AU  - Fontana, Antonella
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2186
AB  - Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence
VL  - 105
IS  - 8
SP  - 2312
EP  - 2320
DO  - 10.1002/jbm.a.36085
ER  - 

@article{
author = "Radunović, Milena and De Colli, Marianna and De Marco, Patrizia and Di Nisio, Chiara and Fontana, Antonella and Piattelli, Adriano and Cataldi, Amelia and Zara, Susi",
year = "2017",
abstract = "Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence",
volume = "105",
number = "8",
pages = "2312-2320",
doi = "10.1002/jbm.a.36085"
}

Radunović, M., De Colli, M., De Marco, P., Di Nisio, C., Fontana, A., Piattelli, A., Cataldi, A.,& Zara, S.. (2017). Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 105(8), 2312-2320.
https://doi.org/10.1002/jbm.a.36085

Radunović M, De Colli M, De Marco P, Di Nisio C, Fontana A, Piattelli A, Cataldi A, Zara S. Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A. 2017;105(8):2312-2320.
doi:10.1002/jbm.a.36085 .

Radunović, Milena, De Colli, Marianna, De Marco, Patrizia, Di Nisio, Chiara, Fontana, Antonella, Piattelli, Adriano, Cataldi, Amelia, Zara, Susi, "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence" in Journal of Biomedical Materials Research Part A, 105, no. 8 (2017):2312-2320,
https://doi.org/10.1002/jbm.a.36085 . .