TY  - JOUR
AU  - De Colli, Marianna
AU  - Radunović, Milena
AU  - Zizzari, Vincenzo L.
AU  - Di Giacomo, Viviana
AU  - Di Nisio, Chiara
AU  - Piattelli, Adriano
AU  - Calvo Guirado, Jose Luis
AU  - Zavan, Barbara
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2304
AB  - Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.
PB  - Japanese Soc Dental Materials Devices, Tokyo
T2  - Dental Materials Journal
T1  - Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface
VL  - 37
IS  - 2
SP  - 197
EP  - 205
DO  - 10.4012/dmj.2016-418
ER  - 

@article{
author = "De Colli, Marianna and Radunović, Milena and Zizzari, Vincenzo L. and Di Giacomo, Viviana and Di Nisio, Chiara and Piattelli, Adriano and Calvo Guirado, Jose Luis and Zavan, Barbara and Cataldi, Amelia and Zara, Susi",
year = "2018",
abstract = "Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.",
publisher = "Japanese Soc Dental Materials Devices, Tokyo",
journal = "Dental Materials Journal",
title = "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface",
volume = "37",
number = "2",
pages = "197-205",
doi = "10.4012/dmj.2016-418"
}

De Colli, M., Radunović, M., Zizzari, V. L., Di Giacomo, V., Di Nisio, C., Piattelli, A., Calvo Guirado, J. L., Zavan, B., Cataldi, A.,& Zara, S.. (2018). Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal
Japanese Soc Dental Materials Devices, Tokyo., 37(2), 197-205.
https://doi.org/10.4012/dmj.2016-418

De Colli M, Radunović M, Zizzari VL, Di Giacomo V, Di Nisio C, Piattelli A, Calvo Guirado JL, Zavan B, Cataldi A, Zara S. Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface. in Dental Materials Journal. 2018;37(2):197-205.
doi:10.4012/dmj.2016-418 .

De Colli, Marianna, Radunović, Milena, Zizzari, Vincenzo L., Di Giacomo, Viviana, Di Nisio, Chiara, Piattelli, Adriano, Calvo Guirado, Jose Luis, Zavan, Barbara, Cataldi, Amelia, Zara, Susi, "Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface" in Dental Materials Journal, 37, no. 2 (2018):197-205,
https://doi.org/10.4012/dmj.2016-418 . .

TY  - JOUR
AU  - De Marco, Patrizia
AU  - Zara, Susi
AU  - De Colli, Marianna
AU  - Radunović, Milena
AU  - Lazović, Vladimir
AU  - Ettorre, Valeria
AU  - Di Crescenzo, Antonello
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Fontana, Antonella
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2218
AB  - Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.
PB  - Iop Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts
VL  - 12
IS  - 5
DO  - 10.1088/1748-605X/aa7907
ER  - 

@article{
author = "De Marco, Patrizia and Zara, Susi and De Colli, Marianna and Radunović, Milena and Lazović, Vladimir and Ettorre, Valeria and Di Crescenzo, Antonello and Piattelli, Adriano and Cataldi, Amelia and Fontana, Antonella",
year = "2017",
abstract = "Commercial collagen membranes are used in oral surgical procedures as scaffolds for bone deposition in guided bone regeneration. Here, we have enriched them with graphene oxide (GO) via a simple non-covalent functionalization, exploiting the capacity of oxygenated carbon functional moieties of GO to interact through hydrogen bonding with collagen. In the present paper, the GO-coated membranes have been characterized in terms of stability, nano-roughness, biocompatibility and induction of inflammatory response in human primary gingival fibroblast cells. The obtained coated membranes are demonstrated not to leak GO in the bulk solution, and to change some features of the membrane, such as stiffness and adhesion between the membrane and the atomic force microscopy (AFM) tip. Moreover, the presence of GO increases the roughness and the total surface exposed to the cells, as demonstrated by AFM analyses. The obtained material is biocompatible, and does not induce inflammation in the tested cells.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts",
volume = "12",
number = "5",
doi = "10.1088/1748-605X/aa7907"
}

De Marco, P., Zara, S., De Colli, M., Radunović, M., Lazović, V., Ettorre, V., Di Crescenzo, A., Piattelli, A., Cataldi, A.,& Fontana, A.. (2017). Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials
Iop Publishing Ltd, Bristol., 12(5).
https://doi.org/10.1088/1748-605X/aa7907

De Marco P, Zara S, De Colli M, Radunović M, Lazović V, Ettorre V, Di Crescenzo A, Piattelli A, Cataldi A, Fontana A. Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts. in Biomedical Materials. 2017;12(5).
doi:10.1088/1748-605X/aa7907 .

De Marco, Patrizia, Zara, Susi, De Colli, Marianna, Radunović, Milena, Lazović, Vladimir, Ettorre, Valeria, Di Crescenzo, Antonello, Piattelli, Adriano, Cataldi, Amelia, Fontana, Antonella, "Graphene oxide improves the biocompatibility of collagen membranes in an in vitro model of human primary gingival fibroblasts" in Biomedical Materials, 12, no. 5 (2017),
https://doi.org/10.1088/1748-605X/aa7907 . .

TY  - JOUR
AU  - Radunović, Milena
AU  - De Colli, Marianna
AU  - De Marco, Patrizia
AU  - Di Nisio, Chiara
AU  - Fontana, Antonella
AU  - Piattelli, Adriano
AU  - Cataldi, Amelia
AU  - Zara, Susi
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2186
AB  - Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.
PB  - Wiley, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence
VL  - 105
IS  - 8
SP  - 2312
EP  - 2320
DO  - 10.1002/jbm.a.36085
ER  - 

@article{
author = "Radunović, Milena and De Colli, Marianna and De Marco, Patrizia and Di Nisio, Chiara and Fontana, Antonella and Piattelli, Adriano and Cataldi, Amelia and Zara, Susi",
year = "2017",
abstract = "Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction. DPSCs were cultured on uncoated membranes and on both 2 and 10 mu g mL(-1) GO coated membranes up to 28 days. Alamar blue and LDH cytotocicity assay, PGE2 ELISA assay, real time RT-PCR for RUNX2, BMP2, SP7, TNF alpha and COX2 genes expression were performed. Proliferation is higher on GO coated membranes at days 14 and 28. LDH assay evidences no cytotoxicity. BMP2 and RUNX2 expression is higher on coated membranes, BMP2 at early and RUNX2 and SP7 at late experimental times. PGE2 levels are lower on GO coated membranes at days 14 and 28, both TNF alpha and COX2 expression is significantly decreased when GO is applied. GO coated membranes are not toxic for DPSCs, induce a faster DPSCs differentiation into odontoblasts/osteoblasts and may represent good alternative to conventional membranes thus ensuring more efficient bone formation and improving the clinical performance.",
publisher = "Wiley, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence",
volume = "105",
number = "8",
pages = "2312-2320",
doi = "10.1002/jbm.a.36085"
}

Radunović, M., De Colli, M., De Marco, P., Di Nisio, C., Fontana, A., Piattelli, A., Cataldi, A.,& Zara, S.. (2017). Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A
Wiley, Hoboken., 105(8), 2312-2320.
https://doi.org/10.1002/jbm.a.36085

Radunović M, De Colli M, De Marco P, Di Nisio C, Fontana A, Piattelli A, Cataldi A, Zara S. Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence. in Journal of Biomedical Materials Research Part A. 2017;105(8):2312-2320.
doi:10.1002/jbm.a.36085 .

Radunović, Milena, De Colli, Marianna, De Marco, Patrizia, Di Nisio, Chiara, Fontana, Antonella, Piattelli, Adriano, Cataldi, Amelia, Zara, Susi, "Graphene oxide enrichment of collagen membranes improves DPSCs differentiation and controls inflammation occurrence" in Journal of Biomedical Materials Research Part A, 105, no. 8 (2017):2312-2320,
https://doi.org/10.1002/jbm.a.36085 . .