Radičević, Goran

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Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization

Dačić, Stefan; Dačić-Simonović, Dragica; Živković, Slavoljub; Dačić, Miloš; Radičević, Goran; Mitić, Aleksandar; Tošić, Goran; Igić, Marko

(Srpsko lekarsko društvo, Beograd, 2014)

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Dačić, Miloš
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Tošić, Goran
AU  - Igić, Marko
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1914
AB  - Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems.
AB  - Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization
T1  - Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije
VL  - 142
IS  - 7-8
SP  - 404
EP  - 412
DO  - 10.2298/SARH1408404D
ER  - 
@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Dačić, Miloš and Radičević, Goran and Mitić, Aleksandar and Tošić, Goran and Igić, Marko",
year = "2014",
abstract = "Introduction Bonding between composite and hard dental tissue is most commonly assessed by measuring bonding strength or absence of marginal gap along the restoration interface. Marginal index (MI) is a significant indicator of the efficiency of the bond between material and dental tissue because it also shows the values of width and length of marginal gap. Objective The aim of this investigation was to estimate quantitative and qualitative features of the bond between composite resin and enamel and to determine the values of MI in enamel after application of two techniques of photopolymerization with two composite systems. Methods Forty Class V cavities on extracted teeth were prepared and restored for scanning electron microscope (SEM) analysis of composite bonding to enamel. Adhesion to enamel was achieved by Adper Single Bond 2 - ASB (3M ESPE), or by Adper Easy One - AEO (3M ESPE). Photopolymerization of Filtek Ultimate - FU (3M ESPE) was performed using constant halogen light (HIP) or soft start program (SOF). Results Quantitative and qualitative analysis, showed better mikromorphological bonding with SOF photopolymerization and ASB/FU composite system. Differences in MI between different photopolymerization techniques (HIP: 0.6707; SOF: 0.2395) were statistically significant (p lt 0.001), as well as differences between the composite systems (ASB/FU: 0.0470; AEO/ FU: 0.8651) (p lt 0.001) by two-way ANOVA test. Conclusion Better marginal adaptation of composite to enamel was obtained with SOF photopolymerization in both composite systems., Uvod Kvalitet veze kompozita za tvrda zubna tkiva se najčešće procenjuje jačinom vezivanja ili izostankom marginalne pukotine po obodu restauracija. Marginalni indeks (MI) je važan pokazatelj efikasnosti pripoja materijala za zubna tkiva jer istovremeno pokazuje vrednosti i širine i dužine marginalne pukotine. Cilj rada Cilj rada je bio da se kvantitativno i kvalitativno proceni veza kompozitnih materijala za gleđ i utvrde vrednosti MI u gleđi nakon primene dve tehnike svetlosne polimerizacije i dva kompozitna sistema. Metode rada Na ekstrahovanim zubima je restaurisano 40 kaviteta pete klase za analizu pripoja kompozita za gleđ skening- elektronskom mikroskopijom (SEM). Adhezija za gleđ je obezbeđivana primenom Adper Single Bond 2 - ASB (3M ESPE), odnosno primenom Adper Easy One - AEO (3M ESPE). Svetlosna polimerizacija kompozitnog materijala (Filtek Ultimate - FU; 3M ESPE) vršena je standardnim halogenim svetlom (HIP) ili tzv. soft start programom (SOF). Rezultati Kvantitativna i kvalitativna analiza je pokazala bolje mikromorfološko vezivanje kompozita za gleđ kod SOF osvetljavanja i ASB/FU kompozitnog sistema. Na osnovu dvosmernog ANOVA testa, razlike u vrednosti MI između različitih svetlosnopolimerizujućih tehnika (HIP: 0,6707 i SOF: 0,2395) bile su statistički značajne (p lt 0,001), kao i razlike između kompozitnih sistema (ASB/FU: 0,0470 i AEO/FU: 0,8651; p lt 0,001). Zaključak Bolji marginalni pripoj kompozita za gleđ obezbeđen je primenom SOF osvetljavanja kod oba kompozitna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization, Analiza ivičnog pripoja kompozitnih smola za gleđ skening-elektronskom mikroskopijom nakon primene standardne i postepene tehnike fotopolimerizacije",
volume = "142",
number = "7-8",
pages = "404-412",
doi = "10.2298/SARH1408404D"
}
Dačić, S., Dačić-Simonović, D., Živković, S., Dačić, M., Radičević, G., Mitić, A., Tošić, G.,& Igić, M.. (2014). Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(7-8), 404-412.
https://doi.org/10.2298/SARH1408404D
Dačić S, Dačić-Simonović D, Živković S, Dačić M, Radičević G, Mitić A, Tošić G, Igić M. Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):404-412.
doi:10.2298/SARH1408404D .
Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Dačić, Miloš, Radičević, Goran, Mitić, Aleksandar, Tošić, Goran, Igić, Marko, "Scanning electron microscopy analysis of marginal adaptation of composite resines to enamel after using of standard and gradual photopolimerization" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):404-412,
https://doi.org/10.2298/SARH1408404D . .
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Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures

Popović, Jelena; Gašić, Jovanka; Živković, Slavoljub; Petrović, A.; Radičević, Goran

(Wiley-Blackwell Publishing, Inc, Malden, 2010)

TY  - JOUR
AU  - Popović, Jelena
AU  - Gašić, Jovanka
AU  - Živković, Slavoljub
AU  - Petrović, A.
AU  - Radičević, Goran
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1529
AB  - Aim To examine the presence of biological debris and the level of contamination on reusable endodontic instruments those were subjected to different cleaning methods prior to sterilization. Methodology One hundred and eighty endodontic instruments from eighteen dental practices were analysed. These practices used different decontamination protocols for reusable instruments. The presence of organic debris was detected by the use of Van Gieson's stain. Forty-eight new stainless steel hand instruments were used as controls. The samples were examined by light microscopy. Results Residual biological debriswasobserved in 96% of the samples. The mean value of maximum biological contamination was 34% in the group in which the instruments were brushed manually and immersed in alcohol, 25% in the group in which commercially available disinfectants were used and 5% in the group in which the instruments were cleaned ultrasonically. There was a statistically significant difference in the mean values with respect to the cleaning protocol applied (P  lt  0.001). Conclusions The methods used to clean endodontic instruments appear to be generally ineffective for the removal of biological debris. The best method was the one that included mechanical, chemical and ultrasonic cleaning of instruments.
PB  - Wiley-Blackwell Publishing, Inc, Malden
T2  - International Endodontic Journal
T1  - Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures
VL  - 43
IS  - 4
SP  - 336
EP  - 341
DO  - 10.1111/j.1365-2591.2010.01686.x
ER  - 
@article{
author = "Popović, Jelena and Gašić, Jovanka and Živković, Slavoljub and Petrović, A. and Radičević, Goran",
year = "2010",
abstract = "Aim To examine the presence of biological debris and the level of contamination on reusable endodontic instruments those were subjected to different cleaning methods prior to sterilization. Methodology One hundred and eighty endodontic instruments from eighteen dental practices were analysed. These practices used different decontamination protocols for reusable instruments. The presence of organic debris was detected by the use of Van Gieson's stain. Forty-eight new stainless steel hand instruments were used as controls. The samples were examined by light microscopy. Results Residual biological debriswasobserved in 96% of the samples. The mean value of maximum biological contamination was 34% in the group in which the instruments were brushed manually and immersed in alcohol, 25% in the group in which commercially available disinfectants were used and 5% in the group in which the instruments were cleaned ultrasonically. There was a statistically significant difference in the mean values with respect to the cleaning protocol applied (P  lt  0.001). Conclusions The methods used to clean endodontic instruments appear to be generally ineffective for the removal of biological debris. The best method was the one that included mechanical, chemical and ultrasonic cleaning of instruments.",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "International Endodontic Journal",
title = "Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures",
volume = "43",
number = "4",
pages = "336-341",
doi = "10.1111/j.1365-2591.2010.01686.x"
}
Popović, J., Gašić, J., Živković, S., Petrović, A.,& Radičević, G.. (2010). Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures. in International Endodontic Journal
Wiley-Blackwell Publishing, Inc, Malden., 43(4), 336-341.
https://doi.org/10.1111/j.1365-2591.2010.01686.x
Popović J, Gašić J, Živković S, Petrović A, Radičević G. Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures. in International Endodontic Journal. 2010;43(4):336-341.
doi:10.1111/j.1365-2591.2010.01686.x .
Popović, Jelena, Gašić, Jovanka, Živković, Slavoljub, Petrović, A., Radičević, Goran, "Evaluation of biological debris on endodontic instruments after cleaning and sterilization procedures" in International Endodontic Journal, 43, no. 4 (2010):336-341,
https://doi.org/10.1111/j.1365-2591.2010.01686.x . .
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SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system

Dačić, Stefan; Dačić-Simonović, Dragica; Živković, Slavoljub; Radičević, Goran; Mitić, Aleksandar; Stanojević, Ivana; Veselinović, Aleksandar

(Srpsko lekarsko društvo, Beograd, 2009)

TY  - JOUR
AU  - Dačić, Stefan
AU  - Dačić-Simonović, Dragica
AU  - Živković, Slavoljub
AU  - Radičević, Goran
AU  - Mitić, Aleksandar
AU  - Stanojević, Ivana
AU  - Veselinović, Aleksandar
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1457
AB  - Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems.
AB  - Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system
T1  - SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema
VL  - 137
IS  - 9-10
SP  - 475
EP  - 481
DO  - 10.2298/SARH0910475D
ER  - 
@article{
author = "Dačić, Stefan and Dačić-Simonović, Dragica and Živković, Slavoljub and Radičević, Goran and Mitić, Aleksandar and Stanojević, Ivana and Veselinović, Aleksandar",
year = "2009",
abstract = "Introduction Quality and durability of enamel/composite interface essentially depend on an adhesive restorative system. Objective The aim of this study was to evaluate the quality of marginal adaptation of composite adhesive systems to enamel by scanning electron microscopy (SEM), and to analyze the morphology of the enamel surface along margins of composite restorations, following exposure to cariogenic solution. Methods The study material included 56 extracted human third molars. Class V cavities were prepared with margins at enamel. Cavities were restored with Single Bond/Z250 and Single Bond/Filtek flow, using the total etch adhesive system, and Prompt-L-Pop/Z250 and Prompt-L-Pop/Filtek flow, using the self etch adhesive system. After the restorative procedure, the restorations were submitted to demineralization during 7 and 28 days. Samples were stored in a cariogenic solution (lactic acid, pH 4.5; 0.1 M) at 37°C or in deionized water (the control group). The margins of restorations and perimarginal enamel were examined by scanning electron microscope (SEM). The gap formations around restoration were measured on cervical, occlusal and approximate margins. Results The usage of the total etch adhesive system showed statistically significant lower marginal gap width around composite restoration, compared with the self etch system (p lt 0.01). The SEM examination also showed perimarginal enamel zones presenting several signs of demineralization and erosion (alteration of rods, porosities) after acting of a cariogenic solution, in both adhesive methods. Less alteration was found on the enamel surfaces not included in the perimarginal zone. Conclusion Treating the cavity with 35% phosphoric acid in the total etch system significantly improved the adaptation of the composite resins to enamel, compared with the self etch treatment. Stronger demineralization of the perimarginal enamel in a cariogenic solution was observed around all restorations in both adhesive systems., Uvod Kvalitet i trajnost veze gleđi i kompozitnih materijala zavisi, između ostalog, i od korišćenog adhezivnog restaurativnog sistema. Cilj rada Cilj istraživanja je bio da se analizom pomoću skening elektronske mikroskopije (SEM) proceni kvalitet ivičnog pripoja kompozitnih adhezivnih sistema i gleđi, odnosno prouče morfološke osobine gleđne površine duž ivica kompozitnih restauracija nakon delovanja kariogenog rastvora. Metode rada Kao materijal u istraživanju korišćeno je 56 ekstrahovanih humanih trećih molara. Na svim zubima su preparirani kaviteti pete klase s ivicama u gleđi. Kaviteti su restaurirani kompozitnim adhezivnim sistemima: Single Bond/Z250 i Single Bond/Filtek Flow, korišćenjem tehnike totalnog nagrizanja, i Prompt-L-Pop/Z250 i Prompt- L-Pop/Filtek Flow, primenom tehnike sa samonagrizajućim adhezivnim sistemima. Posle postupka restauracije zubi su izlagani demineralizaciji tokom sedam i 28 dana potapanjem u kariogeni rastvor (mlečna kiselina pH 4,5; 0,1 M) na 37°C ili držani u dejonizovanoj vodi (kontrolna grupa). Ivice restauracija i perimarginalna gleđ ispitivane su pomoću SEM. Ivična pukotina oko restauracija merena je u predelu vrata zuba, okluzivno i na aproksimalnim stranama. Rezultati Primena tehnike totalnog nagrizanja je pokazala značajno manju širinu ivične pukotine oko kompozitnih restauracija u odnosu na samonagrizajući sistem (p lt 0,01). SEM ispitivanja su takođe otkrila izrazitu demineralizaciju i eroziju ivične zone gleđi (oštećenja prizmi, poroziteti) nakon delovanja kariogenog rastvora kod oba adhezivna sistema. Na gleđnim površinama van perimarginalne zone uočena su neznatna oštećenja. Zaključak Tretman kaviteta sa 35-procentnom fosfornom kiselinom kod totalno nagrizajućeg postupka značajno poboljšava vezu kompozitnih smola za gleđ. Rastvaranje ivične gleđi u kariogenom rastvoru uočeno je oko svih restauracija i kod oba adhezivna sistema.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system, SEM analiza kvaliteta ivičnog pripoja kompozitnih ispuna za gleđ posle primene totalno nagrizajućeg i samonagrizajućeg adhezivnog sistema",
volume = "137",
number = "9-10",
pages = "475-481",
doi = "10.2298/SARH0910475D"
}
Dačić, S., Dačić-Simonović, D., Živković, S., Radičević, G., Mitić, A., Stanojević, I.,& Veselinović, A.. (2009). SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 475-481.
https://doi.org/10.2298/SARH0910475D
Dačić S, Dačić-Simonović D, Živković S, Radičević G, Mitić A, Stanojević I, Veselinović A. SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):475-481.
doi:10.2298/SARH0910475D .
Dačić, Stefan, Dačić-Simonović, Dragica, Živković, Slavoljub, Radičević, Goran, Mitić, Aleksandar, Stanojević, Ivana, Veselinović, Aleksandar, "SEM investigation of composite restoration adaptation to enamel after use of total etch and self etch adhesive system" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):475-481,
https://doi.org/10.2298/SARH0910475D . .
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