TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Milašin, Jelena
AU  - Živković, Slavoljub
AU  - Gašić, Jovanka
AU  - Mitić, Vladimir
AU  - Popović, Jelena
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1795
AB  - Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly.
AB  - Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique
T1  - Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR
VL  - 141
IS  - 3-4
SP  - 155
EP  - 162
DO  - 10.2298/SARH1304155M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Milašin, Jelena and Živković, Slavoljub and Gašić, Jovanka and Mitić, Vladimir and Popović, Jelena",
year = "2013",
abstract = "Introduction. Clinically acceptable antiseptic should possess organolithic-mineralolithic properties and antimicrobial efficacy, and should be non-toxic. Objective. The aim of the paper was to assess the presence of genomes of the most common microorganisms (Porphyromonas gingivalis, Agregatibacter actinomycetemcomitans, Tanerella forsythensis, Prevotella intermedia, Treponema denticola and Enterococcus faecalis) in infected tooth root canals before and after rinsing with solution of doxycycline, citric acid and detergent Tween-80 (MTAD) in patients with clinically diagnosed primary apex periodontitis. Methods. The content of primarily infected canals before and after using the MTAD solution was used as a biological material in which the presence of microorganisms DNA was proved. For the detection of bacterial genome the multiplex PCR technique was applied. Results. The percentage of positive samples before canal treatment was 100%. In infected root canals E. faecalis was most dominant (37%). In a relatively high percentage we detected P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) and P. gingivalis (10%). After rinsing the canal system using MTAD solution, there was a statistically significant decrease in E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) and P. gingivalis (0%). The presence of other bacteria was also diminished but not statistically significantly. Conclusion. With the application of multiplex PCR technique which provided a simultaneous amplification of various genomic sequences, using several pairs of primers, the most dominant in infected root canals were E. faecalis. P. intermedia, A. actinomycetemcomitans, T. denticola, T. forsythensis and P. gingivalis. After mechanic treatment and irrigation of root canals with MTAD solution, P. intermedia, P. gingivalis and T. forsythensis were not found. The presence of E. faecalis, A. actinomycetemcomitans and T. denticola was diminished, however, not statistically significantly., Uvod. Klinički prihvatljiv intrakanalni antiseptik mora imati organolitičko-mineralolitičko dejstvo i antibakterijsku efikasnost i ne sme biti toksičan. Cilj rada. Cilj rada je bio da se ispita postojanje genoma najčešćih mikroorganizama (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythensis, Prevotella intermedia, Treponema denticola i Enterococcus faecalis) u inficiranim kanalima korena zuba pre i posle irigacije rastvorom doksiciklina, limunske kiseline i deterdženta Tween­80 (MTAD) kod osoba s klinički dijagnostikovanim primarnim apeksnim parodontitisom. Metode rada. Kao biološki materijal u kojem je dokazivano prisustvo DNK mikroorganizama korišćen je sadržaj iz primarno inficiranih kanala pre i posle irigacije rastvorom MTAD. Za otkrivanje bakterijskog genoma primenjena je multipleksna tehnika PCR. Rezultati. Procenat pozitivnih uzoraka pre obrade kanala bio je 100%. U inficiranim kanalima korena zuba najčešći je bio E. faecalis (37%). U relativno visokom procentu otkriveni su i: P. intermedia (25%), A. actinomycetemcomitans (20%), T. denticola (17%), T. forsythensis (15%) i P. gingivalis (10%). Posle irigacije kanalnog sistema rastvorom MTAD utvrđeno je statistički značajno smanjenje učestalosti E. faecalis (12%), P. intermedia (0%), T. forsythensis (0%) i P. gingivalis (0%). Učestalost ostalih bakterija takođe se smanjila, ali ne statistički značajno. Zaključak. Primenom multipleksne PCR tehnike, koja omogućava istovremenu amplifikaciju genskih sekvenci uz korišćenje dva para prajmera, u inficiranim kanalima korena zuba najčešće je utvrđen E. faecalis. U relativno visokom procentu otkrivene su P. intermedia, A. actinomycetem­comitans, T. denticola, T. forsythensis i P. gingivalis. Posle hemomehaničke obrade i irigacije kanala rastvorom MTAD nisu zabeležene P. intermedia, P. gingivalis i T. forsythensis, dok je učestalost E. faecalis, A. actinomycetemcomitans i T. denticola smanjena, ali ne statistički značajno.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique, Analiza antimikrobnog dejstva rastvora MTAD u inficiranom kanalnom sistemu korena zuba tehnikom PCR",
volume = "141",
number = "3-4",
pages = "155-162",
doi = "10.2298/SARH1304155M"
}

Mitić, A., Mitić, N., Milašin, J., Živković, S., Gašić, J., Mitić, V.,& Popović, J.. (2013). Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 155-162.
https://doi.org/10.2298/SARH1304155M

Mitić A, Mitić N, Milašin J, Živković S, Gašić J, Mitić V, Popović J. Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):155-162.
doi:10.2298/SARH1304155M .

Mitić, Aleksandar, Mitić, Nadica, Milašin, Jelena, Živković, Slavoljub, Gašić, Jovanka, Mitić, Vladimir, Popović, Jelena, "Analysis of antimicrobial effect of MTAD solution in infected canal system using PCR technique" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):155-162,
https://doi.org/10.2298/SARH1304155M . .

TY  - JOUR
AU  - Mitić, Aleksandar
AU  - Mitić, Nadica
AU  - Živković, Slavoljub
AU  - Tošić, Goran
AU  - Savić, Vojin
AU  - Dačić, Stefan
AU  - Stojanović, Miodrag
PY  - 2009
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1458
AB  - Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system.
AB  - Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Efficiency of final irrigation of root canal in removal of smear layer
T1  - Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja
VL  - 137
IS  - 9-10
SP  - 482
EP  - 489
DO  - 10.2298/SARH0910482M
ER  - 

@article{
author = "Mitić, Aleksandar and Mitić, Nadica and Živković, Slavoljub and Tošić, Goran and Savić, Vojin and Dačić, Stefan and Stojanović, Miodrag",
year = "2009",
abstract = "Introduction A smear layer forms on the root canal walls as the consequence of root instrumentation. The smear layer formed in such a way considerably influences the quality of root obturation and endodontic treatment outcome. Objective The aim of this study was to ultrastructurally analyze the surface of intracanal dentine after removal of the smear layer by the solution of doxycycline, citric acid and detergent Tween-80 (MTAD). Methods The study involved 60 single-rooted, extracted, human teeth divided into four groups. All samples were instrumented by a step-back technique and manual K-files, and rinsed during instrumentation by 2% CHX and H2O2. The first group of samples was exclusively rinsed by CHX and H2O2; in the second group, besides using CHX and H2O2, MTAD solution was used for the final irrigation. The samples which were rinsed by distilled water (+ control) and the samples rinsed by 5.25% NaOCl and 17% Na EDTA (-control) served as control groups. All samples were observed under the scanning electronic microscope JEOL-JSM-5300. The coronary, middle and apex thirds of the radix region were analyzed. Results The obtained results of the SEM analysis showed that the application of 2% chlorhexidine and hydrogen peroxide did not give clear dentine walls, and the smear layer could not be removed completely. The application of the same combination (CHX and H2O2), added by the final MTAD irrigation solution, led to the efficient removal of the smear layer, while the morphological structure of dentine surface remained unchanged. Statistical analysis showed that canal walls in the experimental group with MTAD as the final irrigation were significantly clearer compared to the control group (p lt 0.001). Conclusion Based on the obtained results, it can be concluded that MTAD is an efficient solution for the final irrigation of the canal system., Uvod Razmazni sloj koji se stvara na zidovima kanala korena zuba posledica je instrumentacije kanala. On značajno utiče na kvalitet opturacije kanala i krajnji ishod endodontskog lečenja. Cilj rada Cilj istraživanja je bio da se ustanovi stepen efikasnosti rastvora doksiciklina, limunske kiseline i deterxenta Tween-80 (poznate pod nazivom MTAD) kao završnog irigansa u uklanjanju razmaznog sloja s površina intrakanalnog dentina. Metode rada Ispitano je 60 jednokorenih ekstrahovanih humanih zuba, podeljenih u četiri grupe. Svi uzorci su instrumentirani tehnikom step-back i ručnim K-turpijama, a tokom instrumentacije ispirani dvoprocentnim hlorheksidinom (CHX) i tropostotnim vodonik-peroksidom (H2O2). Prva grupa uzoraka je ispirana isključivo sa CHX i H2O2, dok je za završnu irigaciju uzoraka druge grupe korišćen i rastvor MTAD. Svi uzorci su posmatrani na skening elektronskom mikroskopu JEOL-JSM-5300. Ultrastrukturno su analizirane koronarna, srednja i apikalna trećina korenske regije. Rezultati Primenom samo CHX i H2O2 kao irigansa ne dobijaju se čisti dentinski zidovi, a razmazni sloj sa zidova nije potpuno uklonjen. Istom kombinacijom irigansa koja je dopunjena rastvorom MTAD za završno ispiranje razmazni sloj se efikasno uklanja, a morfološka struktura dentinske površine se ne menja. Statistička analiza je pokazala da su zidovi kanala uzoraka ispiranih i sa MTAD značajno čistiji nego kod uzoraka kontrolne grupe (p lt 0,001). Zaključak Na osnovu rezultata istraživanja može se zaključiti da je MTAD bio najefikasniji rastvor za završnu irigaciju kanalnog sistema korena zuba.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Efficiency of final irrigation of root canal in removal of smear layer, Efikasnost sredstava za završnu irigaciju kanala korena zuba u uklanjanju razmaznog sloja",
volume = "137",
number = "9-10",
pages = "482-489",
doi = "10.2298/SARH0910482M"
}

Mitić, A., Mitić, N., Živković, S., Tošić, G., Savić, V., Dačić, S.,& Stojanović, M.. (2009). Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 137(9-10), 482-489.
https://doi.org/10.2298/SARH0910482M

Mitić A, Mitić N, Živković S, Tošić G, Savić V, Dačić S, Stojanović M. Efficiency of final irrigation of root canal in removal of smear layer. in Srpski arhiv za celokupno lekarstvo. 2009;137(9-10):482-489.
doi:10.2298/SARH0910482M .

Mitić, Aleksandar, Mitić, Nadica, Živković, Slavoljub, Tošić, Goran, Savić, Vojin, Dačić, Stefan, Stojanović, Miodrag, "Efficiency of final irrigation of root canal in removal of smear layer" in Srpski arhiv za celokupno lekarstvo, 137, no. 9-10 (2009):482-489,
https://doi.org/10.2298/SARH0910482M . .

TY  - THES
AU  - Mitić, Nadica
PY  - 1980
UR  - https://plus.sr.cobiss.net/opac7/bib/53341708
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/123
PB  - Univerzitet u Nišu, Stomatološki odsek Medicinskog fakulteta
T1  - Klinički značaj prisustva i difuzije slobodnog eugenola u pasti cink oksid oleum kariofilorum kod terapije dubokih karioznih lezija
UR  - https://hdl.handle.net/21.15107/rcub_smile_123
ER  - 

@phdthesis{
author = "Mitić, Nadica",
year = "1980",
publisher = "Univerzitet u Nišu, Stomatološki odsek Medicinskog fakulteta",
title = "Klinički značaj prisustva i difuzije slobodnog eugenola u pasti cink oksid oleum kariofilorum kod terapije dubokih karioznih lezija",
url = "https://hdl.handle.net/21.15107/rcub_smile_123"
}

Mitić, N.. (1980). Klinički značaj prisustva i difuzije slobodnog eugenola u pasti cink oksid oleum kariofilorum kod terapije dubokih karioznih lezija. 
Univerzitet u Nišu, Stomatološki odsek Medicinskog fakulteta..
https://hdl.handle.net/21.15107/rcub_smile_123

Mitić N. Klinički značaj prisustva i difuzije slobodnog eugenola u pasti cink oksid oleum kariofilorum kod terapije dubokih karioznih lezija. 1980;.
https://hdl.handle.net/21.15107/rcub_smile_123 .

Mitić, Nadica, "Klinički značaj prisustva i difuzije slobodnog eugenola u pasti cink oksid oleum kariofilorum kod terapije dubokih karioznih lezija" (1980),
https://hdl.handle.net/21.15107/rcub_smile_123 .