TY  - JOUR
AU  - Nikolić, Ljubinka
AU  - Čakić, Saša
AU  - Perunović, Neda
AU  - Čolak, Emina
AU  - Kotur-Stevuljević, Jelena
AU  - Janković, Saša
AU  - Đurić, Milanko
AU  - Plećaš, Darko
PY  - 2020
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2496
AB  - Background/Aim. Preterm birth is defined as a delivery prior to the completed 37th week of gestation. Literature data suggested that periodontal processes may influence to the feto-placental unit and induce preterm delivery. The degree of the periodontal disease is influenced by secretor status. Pro-inflammatory cytokines are involved in periodontitis as well as in delivery. The combined influence of these factors on the risk of preterm birth has not been explored. The aim of our study was to investigate the associations between periodontal diseases, secretor status, and interleukin1-b (IL1-ß) and prostaglandine E2 (PGE2) levels in women delivered preterm. Methods. The study included 56 preterm delivery women and 56 women delivered at term as a control group, aged between 17 and 41 years. Periodontal examination, blood and saliva sampling were performed within 48 hours following delivery. Secretor phenotype was determined by hemagglutination inhibition method. The concentrations of IL1-ß and PGE2 were measured by high sensitivity Enzyme-linked Immunosorbent Assay (ELISA). Results. In the pre-term birth group there were 66.1% of women with periodontitis, while in the control one there were 12.5% (p  lt  0.01). Concentrations of IL1-ß and PGE2 in plasma were significantly higher in the non-secretor group of women who gave birth pre-term and had periodontitis comparing to other groups. There was a significant correlation between salivary and plasma levels of PGE2 and IL1-ß in the preterm birth group (R = 0.416, p = 0.017 and R = -0.592, p  lt  0.001, respectively). There were no such correlations in women who delivered at term. Conclusion. Our results support the hypothesis that non-secretor phenotype and periodontitis are at least in part responsible for pathogenesis of preterm birth. This probability of negative impact of non-secretor status cannot be ignored. These findings support the need for additional research into the biology of human parturition.
AB  - Uvod/Cilj. Prevremeni porođaj se definiše kao porođaj pre navršene 37 nedelje gestacije. Podaci iz literature govore u prilog tome da periodontalni procesi mogu uticati na fetoplacentalnu jedinicu i indukovati preterminski porođaj. Sekretorni status može uticati na stepen periodontalne bolesti. Proinflamatorni citokini imaju uticaj na periodontitis kao i na porođaj. Kombinovani uticaj ovih faktora rizika za prevremeni porođaj nije dovoljno istražen. Cilj ove studije je bio da istraži povezanost između periodontalne bolesti, sekretornog statusa, nivoa interleukina 1-b (IL1-b) i prostaglandina E2 (PGE2) kod žena koje su imale prevremeni porođaj. Metode. Studijom je bilo obuhvaćeno 56 žena, koje su imale prevremen porođaj i 56 žena u kontrolnoj grupi koje su se porodile u terminu, starosti između 17 i 41 godine. Periodontalni pregled, uzorkovanje krvi i salive je izvršeno u prvih 48 sati po porođaju. Sekretorni status je određen metodom inhibicije hemaglutinacije. Koncentracije IL1-b i PGE2 su merene visoko senzitivnim Enzyme-linked Immunosorbent Assay (ELISA) testom. Rezultati. U grupi prevremenih porođaja bilo je 66,1% žena sa periodontitisom, a u kontrolnoj grupi 12,5% (p  lt  0.01). Prevremeno porođene žena, nesekretori sa periodontitisom imale su u plazmi značajno više vrednosti IL 1-b i PGE 2 u odnosu na ostale grupe (p  lt  0,01). U grupi prevremeno porođenih žena postojala je značajna korelacija između salivarnih i plazmatskih koncentracija PGE2 i IL1-b (R = 0.416, p = 0.017 i R = -0,592, p  lt  0,001, redom). Ove korelacije nisu postojale kod žena koje su imale terminski porođaj. Zaključak. Naši rezultati podržavaju hipotezu da su sekretorni status i periodontitis, bar delimično, odgovorni za patogenezu preterminskog porođaja. Verovatnoća negativnog uticaja nesekretornog statusa se ne sme ignorisati. Ovi zaključci ukazuju na potrebu za dodatnim istraživanjima porođaja.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis: A cross sectional study
T1  - Salivarni i inflamatorni medijatori plazme i sekretorni status prevremeno porođenih žena sa periodontitisom - studija preseka
VL  - 77
IS  - 3
SP  - 247
EP  - 255
DO  - 10.2298/VSP171106066N
ER  - 

@article{
author = "Nikolić, Ljubinka and Čakić, Saša and Perunović, Neda and Čolak, Emina and Kotur-Stevuljević, Jelena and Janković, Saša and Đurić, Milanko and Plećaš, Darko",
year = "2020",
abstract = "Background/Aim. Preterm birth is defined as a delivery prior to the completed 37th week of gestation. Literature data suggested that periodontal processes may influence to the feto-placental unit and induce preterm delivery. The degree of the periodontal disease is influenced by secretor status. Pro-inflammatory cytokines are involved in periodontitis as well as in delivery. The combined influence of these factors on the risk of preterm birth has not been explored. The aim of our study was to investigate the associations between periodontal diseases, secretor status, and interleukin1-b (IL1-ß) and prostaglandine E2 (PGE2) levels in women delivered preterm. Methods. The study included 56 preterm delivery women and 56 women delivered at term as a control group, aged between 17 and 41 years. Periodontal examination, blood and saliva sampling were performed within 48 hours following delivery. Secretor phenotype was determined by hemagglutination inhibition method. The concentrations of IL1-ß and PGE2 were measured by high sensitivity Enzyme-linked Immunosorbent Assay (ELISA). Results. In the pre-term birth group there were 66.1% of women with periodontitis, while in the control one there were 12.5% (p  lt  0.01). Concentrations of IL1-ß and PGE2 in plasma were significantly higher in the non-secretor group of women who gave birth pre-term and had periodontitis comparing to other groups. There was a significant correlation between salivary and plasma levels of PGE2 and IL1-ß in the preterm birth group (R = 0.416, p = 0.017 and R = -0.592, p  lt  0.001, respectively). There were no such correlations in women who delivered at term. Conclusion. Our results support the hypothesis that non-secretor phenotype and periodontitis are at least in part responsible for pathogenesis of preterm birth. This probability of negative impact of non-secretor status cannot be ignored. These findings support the need for additional research into the biology of human parturition., Uvod/Cilj. Prevremeni porođaj se definiše kao porođaj pre navršene 37 nedelje gestacije. Podaci iz literature govore u prilog tome da periodontalni procesi mogu uticati na fetoplacentalnu jedinicu i indukovati preterminski porođaj. Sekretorni status može uticati na stepen periodontalne bolesti. Proinflamatorni citokini imaju uticaj na periodontitis kao i na porođaj. Kombinovani uticaj ovih faktora rizika za prevremeni porođaj nije dovoljno istražen. Cilj ove studije je bio da istraži povezanost između periodontalne bolesti, sekretornog statusa, nivoa interleukina 1-b (IL1-b) i prostaglandina E2 (PGE2) kod žena koje su imale prevremeni porođaj. Metode. Studijom je bilo obuhvaćeno 56 žena, koje su imale prevremen porođaj i 56 žena u kontrolnoj grupi koje su se porodile u terminu, starosti između 17 i 41 godine. Periodontalni pregled, uzorkovanje krvi i salive je izvršeno u prvih 48 sati po porođaju. Sekretorni status je određen metodom inhibicije hemaglutinacije. Koncentracije IL1-b i PGE2 su merene visoko senzitivnim Enzyme-linked Immunosorbent Assay (ELISA) testom. Rezultati. U grupi prevremenih porođaja bilo je 66,1% žena sa periodontitisom, a u kontrolnoj grupi 12,5% (p  lt  0.01). Prevremeno porođene žena, nesekretori sa periodontitisom imale su u plazmi značajno više vrednosti IL 1-b i PGE 2 u odnosu na ostale grupe (p  lt  0,01). U grupi prevremeno porođenih žena postojala je značajna korelacija između salivarnih i plazmatskih koncentracija PGE2 i IL1-b (R = 0.416, p = 0.017 i R = -0,592, p  lt  0,001, redom). Ove korelacije nisu postojale kod žena koje su imale terminski porođaj. Zaključak. Naši rezultati podržavaju hipotezu da su sekretorni status i periodontitis, bar delimično, odgovorni za patogenezu preterminskog porođaja. Verovatnoća negativnog uticaja nesekretornog statusa se ne sme ignorisati. Ovi zaključci ukazuju na potrebu za dodatnim istraživanjima porođaja.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis: A cross sectional study, Salivarni i inflamatorni medijatori plazme i sekretorni status prevremeno porođenih žena sa periodontitisom - studija preseka",
volume = "77",
number = "3",
pages = "247-255",
doi = "10.2298/VSP171106066N"
}

Nikolić, L., Čakić, S., Perunović, N., Čolak, E., Kotur-Stevuljević, J., Janković, S., Đurić, M.,& Plećaš, D.. (2020). Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis: A cross sectional study. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 77(3), 247-255.
https://doi.org/10.2298/VSP171106066N

Nikolić L, Čakić S, Perunović N, Čolak E, Kotur-Stevuljević J, Janković S, Đurić M, Plećaš D. Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis: A cross sectional study. in Vojnosanitetski pregled. 2020;77(3):247-255.
doi:10.2298/VSP171106066N .

Nikolić, Ljubinka, Čakić, Saša, Perunović, Neda, Čolak, Emina, Kotur-Stevuljević, Jelena, Janković, Saša, Đurić, Milanko, Plećaš, Darko, "Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis: A cross sectional study" in Vojnosanitetski pregled, 77, no. 3 (2020):247-255,
https://doi.org/10.2298/VSP171106066N . .

TY  - JOUR
AU  - Novaković, Nada
AU  - Čakić, Saša
AU  - Todorović, Tatjana
AU  - Anđelski-Radičević, Biljana
AU  - Dožić, Ivan
AU  - Petrović, Vanja
AU  - Perunović, Neda
AU  - Špadijer-Gostović, Aleksandra
AU  - Kadović-Sretenović, Jana
AU  - Čolak, Emina
PY  - 2013
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1796
AB  - Introduction. Oxidative stress and antioxidants play an important role in the pathogenesis of inflammatory disease, including chronic periodontitis (CP). Saliva contains enzymatic (glutathione peroxidase - GPx, superoxide dismutase - SOD, etc.) and non-enzymatic (albumin - ALB, uric acid - UA, glutathione, etc.) antioxidants. Objective. The aims of this study were to investigate: a) level of SOD, GPx, UA, ALB and total antioxidative status (TAS) of saliva in CP patients before and after non-surgical treatment, and b) correlations between clinical periodontal parameters and levels of salivary antioxidants. Methods. Saliva was collected from 21 CP patients before and after non-surgical treatment. The condition of periodontium was assessed by plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss. Level of investigated antioxidants (except GPx) and TAS was determined using colorimetric method and commercial kits. GPx activity was determined using UV method and commercial kits. Results. After the treatment significant increase of UA, ALB, Gpx, TAS was detected (p lt 0.01) and decrease of SOD activity (p>0.05). A significant correlation was observed between GPx and PI (r=0.575, p=0.008), SOD and GI (r=0.525, p=0.017) before therapy, and SOD and bleeding on probing (BP) (r=0.59, p=0.006), TAS and BP (r=0.453, p=0.045) after therapy. Conclusion. These data suggest that levels of salivary antioxidants generally increase after non-surgical periodontal treatment. Correlation between some clinical periodontal parameters and level of salivary antioxidants was found.
AB  - Uvod. Oksidativni stres i antioksidansi igraju važnu ulogu u patogenezi zapaljenjskih oboljenja, uključujući i hroničnu parodontopatiju. Pljuvačka sadrži enzimske antioksidanse, kao što su glutation-peroksidaza (GPx) i superoksid-dismutaza (SOD), i neenzimske antioksidanse, poput albumina (ALB), mokraćne kiseline (UA), glutationa itd. Cilj rada. Cilj istraživanja bio je da se ispitaju nivoi SOD, GPx, UA i ALB i utvrdi ukupan antioksidantni status pljuvačke (TAS) kod osoba s hroničnom parodontopatijom pre i posle kauzalne terapije, te ustanove korelacije između kliničkih pokazatelja stanja parodoncijuma i nivoa antioksidansa u pljuvački. Metode rada. Pljuvačka je sakupljena od 21 pacijenta s hroničnom parodontopatijom pre i posle kauzalne terapije. Stanje parodoncijuma je procenjivano pomoću: plak-indeksa (PI), gingivalnog indeksa (GI), indeksa krvarenja gingive (BP), dubine parodontalnog džepa i nivoa pripojnog epitela. Nivo ispitivanih antioksidansa (osim GPx) i TAS određivan je pomoću kolorimetrijske metode i komercijalnih reagensa. GPx je određivan primenom UV metode i komercijalnih reagensa. Rezultati. Nakon terapije ustanovljeni su značajno povećanje koncentracije UA, GPx i TAS (p lt 0,01) i smanjenje aktivnosti SOD (p>0,05). Primećena je i značajna korelacija između GPx i PI (r=0,575; p=0,008), te SOD i GI (r=0,525; p=0,017) pre terapije, odnosno SOD i BP (r=0,59; p=0,006), kao i TAS i BP (r=0,453; p=0,045) posle nje. Zaključak. Dobijeni nalazi pokazuju da se nivoi antioksidansa u pljuvački povećavaju nakon kauzalne terapije parodontopatije. Uočene su pozitivne korelacije između kliničkih pokazatelja stanja parodoncijuma i ispitivanih koncentracija antioksidansa u pljuvački.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Antioxidative status of saliva before and after non-surgical periodontal treatment
T1  - Antioksidantni status pljuvačke pre i posle kauzalne terapije parodontopatije
VL  - 141
IS  - 3-4
SP  - 163
EP  - 168
DO  - 10.2298/SARH1304163N
ER  - 

@article{
author = "Novaković, Nada and Čakić, Saša and Todorović, Tatjana and Anđelski-Radičević, Biljana and Dožić, Ivan and Petrović, Vanja and Perunović, Neda and Špadijer-Gostović, Aleksandra and Kadović-Sretenović, Jana and Čolak, Emina",
year = "2013",
abstract = "Introduction. Oxidative stress and antioxidants play an important role in the pathogenesis of inflammatory disease, including chronic periodontitis (CP). Saliva contains enzymatic (glutathione peroxidase - GPx, superoxide dismutase - SOD, etc.) and non-enzymatic (albumin - ALB, uric acid - UA, glutathione, etc.) antioxidants. Objective. The aims of this study were to investigate: a) level of SOD, GPx, UA, ALB and total antioxidative status (TAS) of saliva in CP patients before and after non-surgical treatment, and b) correlations between clinical periodontal parameters and levels of salivary antioxidants. Methods. Saliva was collected from 21 CP patients before and after non-surgical treatment. The condition of periodontium was assessed by plaque index, gingival index, bleeding on probing, probing depth and clinical attachment loss. Level of investigated antioxidants (except GPx) and TAS was determined using colorimetric method and commercial kits. GPx activity was determined using UV method and commercial kits. Results. After the treatment significant increase of UA, ALB, Gpx, TAS was detected (p lt 0.01) and decrease of SOD activity (p>0.05). A significant correlation was observed between GPx and PI (r=0.575, p=0.008), SOD and GI (r=0.525, p=0.017) before therapy, and SOD and bleeding on probing (BP) (r=0.59, p=0.006), TAS and BP (r=0.453, p=0.045) after therapy. Conclusion. These data suggest that levels of salivary antioxidants generally increase after non-surgical periodontal treatment. Correlation between some clinical periodontal parameters and level of salivary antioxidants was found., Uvod. Oksidativni stres i antioksidansi igraju važnu ulogu u patogenezi zapaljenjskih oboljenja, uključujući i hroničnu parodontopatiju. Pljuvačka sadrži enzimske antioksidanse, kao što su glutation-peroksidaza (GPx) i superoksid-dismutaza (SOD), i neenzimske antioksidanse, poput albumina (ALB), mokraćne kiseline (UA), glutationa itd. Cilj rada. Cilj istraživanja bio je da se ispitaju nivoi SOD, GPx, UA i ALB i utvrdi ukupan antioksidantni status pljuvačke (TAS) kod osoba s hroničnom parodontopatijom pre i posle kauzalne terapije, te ustanove korelacije između kliničkih pokazatelja stanja parodoncijuma i nivoa antioksidansa u pljuvački. Metode rada. Pljuvačka je sakupljena od 21 pacijenta s hroničnom parodontopatijom pre i posle kauzalne terapije. Stanje parodoncijuma je procenjivano pomoću: plak-indeksa (PI), gingivalnog indeksa (GI), indeksa krvarenja gingive (BP), dubine parodontalnog džepa i nivoa pripojnog epitela. Nivo ispitivanih antioksidansa (osim GPx) i TAS određivan je pomoću kolorimetrijske metode i komercijalnih reagensa. GPx je određivan primenom UV metode i komercijalnih reagensa. Rezultati. Nakon terapije ustanovljeni su značajno povećanje koncentracije UA, GPx i TAS (p lt 0,01) i smanjenje aktivnosti SOD (p>0,05). Primećena je i značajna korelacija između GPx i PI (r=0,575; p=0,008), te SOD i GI (r=0,525; p=0,017) pre terapije, odnosno SOD i BP (r=0,59; p=0,006), kao i TAS i BP (r=0,453; p=0,045) posle nje. Zaključak. Dobijeni nalazi pokazuju da se nivoi antioksidansa u pljuvački povećavaju nakon kauzalne terapije parodontopatije. Uočene su pozitivne korelacije između kliničkih pokazatelja stanja parodoncijuma i ispitivanih koncentracija antioksidansa u pljuvački.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Antioxidative status of saliva before and after non-surgical periodontal treatment, Antioksidantni status pljuvačke pre i posle kauzalne terapije parodontopatije",
volume = "141",
number = "3-4",
pages = "163-168",
doi = "10.2298/SARH1304163N"
}

Novaković, N., Čakić, S., Todorović, T., Anđelski-Radičević, B., Dožić, I., Petrović, V., Perunović, N., Špadijer-Gostović, A., Kadović-Sretenović, J.,& Čolak, E.. (2013). Antioxidative status of saliva before and after non-surgical periodontal treatment. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 163-168.
https://doi.org/10.2298/SARH1304163N

Novaković N, Čakić S, Todorović T, Anđelski-Radičević B, Dožić I, Petrović V, Perunović N, Špadijer-Gostović A, Kadović-Sretenović J, Čolak E. Antioxidative status of saliva before and after non-surgical periodontal treatment. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):163-168.
doi:10.2298/SARH1304163N .

Novaković, Nada, Čakić, Saša, Todorović, Tatjana, Anđelski-Radičević, Biljana, Dožić, Ivan, Petrović, Vanja, Perunović, Neda, Špadijer-Gostović, Aleksandra, Kadović-Sretenović, Jana, Čolak, Emina, "Antioxidative status of saliva before and after non-surgical periodontal treatment" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):163-168,
https://doi.org/10.2298/SARH1304163N . .