TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Ilić, Vesna
AU  - Mojsilović, Sonja
AU  - Marković, Dragana
AU  - Milošević-Jovčić, Nadežda
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2046
AB  - Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects.
AB  - Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease
T1  - Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije
VL  - 62
IS  - 2
SP  - 55
EP  - 64
DO  - 10.1515/sdj-2015-0006
ER  - 

@article{
author = "Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Ilić, Vesna and Mojsilović, Sonja and Marković, Dragana and Milošević-Jovčić, Nadežda",
year = "2015",
abstract = "Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects., Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease, Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije",
volume = "62",
number = "2",
pages = "55-64",
doi = "10.1515/sdj-2015-0006"
}

Brajović, G., Nikolić-Jakoba, N., Popović, B., Ilić, V., Mojsilović, S., Marković, D.,& Milošević-Jovčić, N.. (2015). Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(2), 55-64.
https://doi.org/10.1515/sdj-2015-0006

Brajović G, Nikolić-Jakoba N, Popović B, Ilić V, Mojsilović S, Marković D, Milošević-Jovčić N. Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije. 2015;62(2):55-64.
doi:10.1515/sdj-2015-0006 .

Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Ilić, Vesna, Mojsilović, Sonja, Marković, Dragana, Milošević-Jovčić, Nadežda, "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease" in Stomatološki glasnik Srbije, 62, no. 2 (2015):55-64,
https://doi.org/10.1515/sdj-2015-0006 . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Stefanović, Gordana
AU  - Ilić, Vesna
AU  - Petrović, Sonja
AU  - Stefanović, Neda
AU  - Nikolić-Jakoba, Nataša
AU  - Milošević-Jovčić, Nadežda
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1563
AB  - Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis
VL  - 81
IS  - 10
SP  - 1472
EP  - 1480
DO  - 10.1902/jop.2010.100053
ER  - 

@article{
author = "Brajović, Gavrilo and Stefanović, Gordana and Ilić, Vesna and Petrović, Sonja and Stefanović, Neda and Nikolić-Jakoba, Nataša and Milošević-Jovčić, Nadežda",
year = "2010",
abstract = "Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis",
volume = "81",
number = "10",
pages = "1472-1480",
doi = "10.1902/jop.2010.100053"
}

Brajović, G., Stefanović, G., Ilić, V., Petrović, S., Stefanović, N., Nikolić-Jakoba, N.,& Milošević-Jovčić, N.. (2010). Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology
Wiley, Hoboken., 81(10), 1472-1480.
https://doi.org/10.1902/jop.2010.100053

Brajović G, Stefanović G, Ilić V, Petrović S, Stefanović N, Nikolić-Jakoba N, Milošević-Jovčić N. Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology. 2010;81(10):1472-1480.
doi:10.1902/jop.2010.100053 .

Brajović, Gavrilo, Stefanović, Gordana, Ilić, Vesna, Petrović, Sonja, Stefanović, Neda, Nikolić-Jakoba, Nataša, Milošević-Jovčić, Nadežda, "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis" in Journal of Periodontology, 81, no. 10 (2010):1472-1480,
https://doi.org/10.1902/jop.2010.100053 . .

TY  - JOUR
AU  - Todorović, Vera
AU  - Marković, Dejan
AU  - Milošević-Jovčić, Nadežda
AU  - Petakov, Marijana
AU  - Balint, Bela
AU  - Čolić, Miodrag
AU  - Milenković, Ana
AU  - Čolak, Ivana
AU  - Jokanović, Vukoman
AU  - Nikolić, Nebojša
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1412
AB  - To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages.
AB  - Iz zubne pulpe su do danas izolovane tri populacije matičnih ćelija koje su označene kao matične ćelije zubne pulpe (engl. Dental Pulp Stem Cells, DPSC), matične ćelije iz eksfoliranih mlečnih zuba (engl. Stem Cells From Human Exfoliated Decidual Teeth, SHED) i nezrele matične ćelije zubne pulpe (engl. Immature Dental Pulp Stem Cells, IDPC). Sve matične ćelije zubne pulpe su ektomezenhimalnog porekla i lokalizovane su u perivaskularnoj niši. One se lako i efikasno izoluju, visoko su proliferativne, klonogene, multipotentne, ispoljavaju visok stepen plasticiteta i i slične su mezenhimalnim matičnim ćelijama koštane srži (BMSC). U njima je pokazana visoka ekspresija gena alkalne fosfataze, proteina 1 matriksa dentina i dentin-sijalofosfoproteina. Takođe, istaknuta je važnost u ovim ćelijama ekspresije više gena koji kodiraju sintezu komponenti ekstracelularnog matriksa, molekula ćelijske adhezije, faktora rasta, transkripcionih faktora, gena prenosa ćelijskih signala, ćelijske komunikacije i metabolizma. U uslovima in vitro ili in vivo ove ćelije mogu da se diferenciraju, s određenim međusobnim razlikama, u pravcu odontoblasta, hondrocita, osteoblasta, adipocita, neurona/glije, glatkih i skeletnih mišićnih ćelija, endotelnih ćelija i melanocita. U uslovima in vivo, nakon implantacije, pokazuju različit potencijal za formiranje dentina, ali i koštanog, masnog i nervnog tkiva. Generalno se smatra da DPSC imaju anti-inflamatorno dejstvo i ispoljavaju imunom-odulatorni efekat. Takođe, dovode do imunološke tolerancije ukoliko se implantiraju u alogena tkiva. Sposobnost inhibicije proliferacije T limfocita ukazuje na njihovo imunosupresivno dejstvo. Matične ćelije zubne pulpe otvorile su nove perspektive u terapijskoj primeni ovih ćelija ne samo u regeneraciji dentina, tkiva periodoncijuma i koštano-zglobnog tkiva kraniofacijalne regije, već i u lečenju neurotraume, autoimunskih oboljenja, infarkta miokarda, mišićne distrofije i oštećenja vezivnog tkiva.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Dental pulp stem cells: Potential significance in regenerative medicine
T1  - Matične ćelije zubne pulpe i njihov potencijalni značaj u regenerativnoj medicini
VL  - 55
IS  - 3
SP  - 170
EP  - 179
DO  - 10.2298/SGS0803170T
ER  - 

@article{
author = "Todorović, Vera and Marković, Dejan and Milošević-Jovčić, Nadežda and Petakov, Marijana and Balint, Bela and Čolić, Miodrag and Milenković, Ana and Čolak, Ivana and Jokanović, Vukoman and Nikolić, Nebojša",
year = "2008",
abstract = "To date, three types of dental stem cells have been isolated: Dental Pulp Stem Cells (DPSC), Stem Cells From Human Exfoliated Deciduous Teeth (SHED) and Immature Dental Pulp Stem Cells (IDPC). These dental stem cells are considered as mesenchymal stem cells. They reside within the perivascular niche of dental pulp. They are highly proliferative, clonogenic, multipotent and are similar to mesenchymal Bone Marrow Stem Cells (BMSC). Also, they have high plasticity and can be easy isolated. The expressions of the alkaline phosphatase gene, dentin matrix protein 1 and dentinsialophosphoprotein are verified in these cells. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors and transcription regulators, cell signaling, cell communication or cell metabolism. In both conditions, in vivo and in vitro, these cells have the ability to differentiate into odontoblasts, chondrocytes, osteoblasts, adipocytes, neurons, melanocytes, smooth and skeletal muscles and endothelial cells. In vivo, after implantation, they have shown potential to differentiate into dentin but also into tissues like bone, adipose or neural tissue. In general, DPSCs are considered to have antiinflammatory and immunomodulatory abilities. After being grafted into allogenic tissues these cells are ableto induce immunological tolerance. Immunosuppressive effect is shown through the ability to inhibit proliferation of T lymphocytes. Dental pulp stem cells open new perspectives in therapeutic use not only in dentin regeneration, periodontal tissues and skeletoarticular, tissues of craniofacial region but also in treatment of neurotrauma, autoimmune diseases, myocardial infarction, muscular dystrophy and connective tissue damages., Iz zubne pulpe su do danas izolovane tri populacije matičnih ćelija koje su označene kao matične ćelije zubne pulpe (engl. Dental Pulp Stem Cells, DPSC), matične ćelije iz eksfoliranih mlečnih zuba (engl. Stem Cells From Human Exfoliated Decidual Teeth, SHED) i nezrele matične ćelije zubne pulpe (engl. Immature Dental Pulp Stem Cells, IDPC). Sve matične ćelije zubne pulpe su ektomezenhimalnog porekla i lokalizovane su u perivaskularnoj niši. One se lako i efikasno izoluju, visoko su proliferativne, klonogene, multipotentne, ispoljavaju visok stepen plasticiteta i i slične su mezenhimalnim matičnim ćelijama koštane srži (BMSC). U njima je pokazana visoka ekspresija gena alkalne fosfataze, proteina 1 matriksa dentina i dentin-sijalofosfoproteina. Takođe, istaknuta je važnost u ovim ćelijama ekspresije više gena koji kodiraju sintezu komponenti ekstracelularnog matriksa, molekula ćelijske adhezije, faktora rasta, transkripcionih faktora, gena prenosa ćelijskih signala, ćelijske komunikacije i metabolizma. U uslovima in vitro ili in vivo ove ćelije mogu da se diferenciraju, s određenim međusobnim razlikama, u pravcu odontoblasta, hondrocita, osteoblasta, adipocita, neurona/glije, glatkih i skeletnih mišićnih ćelija, endotelnih ćelija i melanocita. U uslovima in vivo, nakon implantacije, pokazuju različit potencijal za formiranje dentina, ali i koštanog, masnog i nervnog tkiva. Generalno se smatra da DPSC imaju anti-inflamatorno dejstvo i ispoljavaju imunom-odulatorni efekat. Takođe, dovode do imunološke tolerancije ukoliko se implantiraju u alogena tkiva. Sposobnost inhibicije proliferacije T limfocita ukazuje na njihovo imunosupresivno dejstvo. Matične ćelije zubne pulpe otvorile su nove perspektive u terapijskoj primeni ovih ćelija ne samo u regeneraciji dentina, tkiva periodoncijuma i koštano-zglobnog tkiva kraniofacijalne regije, već i u lečenju neurotraume, autoimunskih oboljenja, infarkta miokarda, mišićne distrofije i oštećenja vezivnog tkiva.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Dental pulp stem cells: Potential significance in regenerative medicine, Matične ćelije zubne pulpe i njihov potencijalni značaj u regenerativnoj medicini",
volume = "55",
number = "3",
pages = "170-179",
doi = "10.2298/SGS0803170T"
}

Todorović, V., Marković, D., Milošević-Jovčić, N., Petakov, M., Balint, B., Čolić, M., Milenković, A., Čolak, I., Jokanović, V.,& Nikolić, N.. (2008). Dental pulp stem cells: Potential significance in regenerative medicine. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 55(3), 170-179.
https://doi.org/10.2298/SGS0803170T

Todorović V, Marković D, Milošević-Jovčić N, Petakov M, Balint B, Čolić M, Milenković A, Čolak I, Jokanović V, Nikolić N. Dental pulp stem cells: Potential significance in regenerative medicine. in Stomatološki glasnik Srbije. 2008;55(3):170-179.
doi:10.2298/SGS0803170T .

Todorović, Vera, Marković, Dejan, Milošević-Jovčić, Nadežda, Petakov, Marijana, Balint, Bela, Čolić, Miodrag, Milenković, Ana, Čolak, Ivana, Jokanović, Vukoman, Nikolić, Nebojša, "Dental pulp stem cells: Potential significance in regenerative medicine" in Stomatološki glasnik Srbije, 55, no. 3 (2008):170-179,
https://doi.org/10.2298/SGS0803170T . .

TY  - JOUR
AU  - Ilić, Vesna
AU  - Milošević-Jovčić, Nadežda
AU  - Marković, D.
AU  - Petrović, S.
AU  - Stefanović, Gordana
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1346
AB  - The association between a particular Gm haplotype and susceptibility to multiple myeloma (MM) is not clear. The reason is probably because no investigations have so far been carried out on the relationship between the Gm haplotype, which represents the inherited combination of IgG Gm allotypes, and the Gm allotype expressed at the IgG paraprotein (M-component), which reflects the enhanced gene expression within the haplotype in MM. We studied the incidence of Gm allotypic markers present in IgG subclasses in the serum from 52 patients with MM and in parallel with the isolated IgG paraproteins. The results showed that 84.6% of the patients were heterozygous for haplotypes Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) and 15.3% were homozygous for Gm(f; n/n-; b1; b0; b5), while no homozygous Gm(a; z; n-; g) individuals were found among the studied patients. The incidence of these combinations in the healthy population in Serbia is 34%, 66% and  lt  1%, respectively. Subjects with Gm(a; z; n-; g)/Gm(f; n+/n-; b1; b0; b5) combination are over 10 times [odds ratio (OR) = 10.69; 95% confidence interval 1.67-68] as likely to be affected by the disease as the subjects with homozygous Gm(f; n+/n-; b1; b0; b5) combination (OR = 0.35, 95% confidence interval 0.06-2.23). However, despite the Gm heterozygosity, most of the Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) positive patients with MM (86.3%) had IgG paraprotein with the allotypic marker from the Gm(f; n+/n-; b1; b0; b5) haplotype. Together with patients homozygous for this haplotype, the relative number of patients with serum IgG paraprotein carrying allotypic marker from the Gm(f; n/n-; b1; b0; b5) haplotype was 88.5%. These results suggest that the development of an M-component could be related to a disturbance on chromosome 14q32 carrying the Gm (f; n+/n-; b1; b0; b5) set of genes.
PB  - Wiley, Hoboken
T2  - International Journal of Immunogenetics
T1  - A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development
VL  - 34
IS  - 2
SP  - 119
EP  - 125
DO  - 10.1111/j.1744-313X.2007.00673.x
ER  - 

@article{
author = "Ilić, Vesna and Milošević-Jovčić, Nadežda and Marković, D. and Petrović, S. and Stefanović, Gordana",
year = "2007",
abstract = "The association between a particular Gm haplotype and susceptibility to multiple myeloma (MM) is not clear. The reason is probably because no investigations have so far been carried out on the relationship between the Gm haplotype, which represents the inherited combination of IgG Gm allotypes, and the Gm allotype expressed at the IgG paraprotein (M-component), which reflects the enhanced gene expression within the haplotype in MM. We studied the incidence of Gm allotypic markers present in IgG subclasses in the serum from 52 patients with MM and in parallel with the isolated IgG paraproteins. The results showed that 84.6% of the patients were heterozygous for haplotypes Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) and 15.3% were homozygous for Gm(f; n/n-; b1; b0; b5), while no homozygous Gm(a; z; n-; g) individuals were found among the studied patients. The incidence of these combinations in the healthy population in Serbia is 34%, 66% and  lt  1%, respectively. Subjects with Gm(a; z; n-; g)/Gm(f; n+/n-; b1; b0; b5) combination are over 10 times [odds ratio (OR) = 10.69; 95% confidence interval 1.67-68] as likely to be affected by the disease as the subjects with homozygous Gm(f; n+/n-; b1; b0; b5) combination (OR = 0.35, 95% confidence interval 0.06-2.23). However, despite the Gm heterozygosity, most of the Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) positive patients with MM (86.3%) had IgG paraprotein with the allotypic marker from the Gm(f; n+/n-; b1; b0; b5) haplotype. Together with patients homozygous for this haplotype, the relative number of patients with serum IgG paraprotein carrying allotypic marker from the Gm(f; n/n-; b1; b0; b5) haplotype was 88.5%. These results suggest that the development of an M-component could be related to a disturbance on chromosome 14q32 carrying the Gm (f; n+/n-; b1; b0; b5) set of genes.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Immunogenetics",
title = "A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development",
volume = "34",
number = "2",
pages = "119-125",
doi = "10.1111/j.1744-313X.2007.00673.x"
}

Ilić, V., Milošević-Jovčić, N., Marković, D., Petrović, S.,& Stefanović, G.. (2007). A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development. in International Journal of Immunogenetics
Wiley, Hoboken., 34(2), 119-125.
https://doi.org/10.1111/j.1744-313X.2007.00673.x

Ilić V, Milošević-Jovčić N, Marković D, Petrović S, Stefanović G. A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development. in International Journal of Immunogenetics. 2007;34(2):119-125.
doi:10.1111/j.1744-313X.2007.00673.x .

Ilić, Vesna, Milošević-Jovčić, Nadežda, Marković, D., Petrović, S., Stefanović, Gordana, "A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development" in International Journal of Immunogenetics, 34, no. 2 (2007):119-125,
https://doi.org/10.1111/j.1744-313X.2007.00673.x . .

TY  - JOUR
AU  - Stefanović, Gordana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1318
AB  - Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.
PB  - Amer Acad Periodontology, Chicago
T2  - Journal of Periodontology
T1  - Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis
VL  - 77
IS  - 11
SP  - 1887
EP  - 1893
DO  - 10.1902/jop.2006.060049
ER  - 

@article{
author = "Stefanović, Gordana and Marković, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.",
publisher = "Amer Acad Periodontology, Chicago",
journal = "Journal of Periodontology",
title = "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis",
volume = "77",
number = "11",
pages = "1887-1893",
doi = "10.1902/jop.2006.060049"
}

Stefanović, G., Marković, D., Ilić, V., Brajović, G., Petrović, S.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology
Amer Acad Periodontology, Chicago., 77(11), 1887-1893.
https://doi.org/10.1902/jop.2006.060049

Stefanović G, Marković D, Ilić V, Brajović G, Petrović S, Milošević-Jovčić N. Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology. 2006;77(11):1887-1893.
doi:10.1902/jop.2006.060049 .

Stefanović, Gordana, Marković, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević-Jovčić, Nadežda, "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis" in Journal of Periodontology, 77, no. 11 (2006):1887-1893,
https://doi.org/10.1902/jop.2006.060049 . .

TY  - JOUR
AU  - Stefanović, Gordana
AU  - Ćirić, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević, Dragan
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1310
AB  - Changed glycosylation of immunoglobulin G (IgG), above all, the expression of thermal galactose, influence to numerous functions of those immunoglobulin and correlate with the inflammatory level in a number of diseases. Aim: This work analyses the distribution of IgG subclasses and the content of thermal galactose in them, in saliva and gingival fluid of the patients with periodontal disease and different gum inflammatory level. Materials and methods: It was used saliva and gingival fluid of 30 adults with clinical picture of periodontal disease and 20 persons with healthy periodontium. The qualification of IgG was done by “dot-blot” procedure and the, and thermal galactose was determined by lectin immunoblot procedure. Results: The division of IgG subclasses in both fluids was different in the patients with periodontal disease and in control samples. In saliva and gingival fluid of the diseased quantitatively dominated IgG2 subclasses, independently from periodontal status. In IgG of both fluids, thermal galactose was exprimated at the healthy periodontium persons (control) and with the person with initial periodontal disease, while at the person with increased periodontal disease the expression of this saccharide wasn’t registered in neither of fluids. Conclusion: The results showed that there is a shift towards hypogalactosyled IgG glikoforms during the process of gum inflammation at the periodontal disease patients.
AB  - Promenjena glikozilacija imunoglobulina G (IgG), pre svega ekspresija terminalne galaktoze, utiče na brojne funkcije ovih imunoglobulina i korelira sa stepenom zapaljenja u mnogim bolestima. Cilj rada: U ovom radu analizirana je distribucija IgG podklasa i sadržaj terminalne galaktoze u njima, u pljuvačci i gingivalnoj tečnosti bolesnika sa parodontopatijom različitog stepena inflamacije gingive. Materijal i metod: Kao materijal u ispitivanjima korišćena je gingivalna tečnost i pljuvačka 30 odraslih osoba sa kliničkom dijagnozom parodontopatija i 20 osoba sa zdravim parodoncijumom. Kvantifikacija IgG urađena je “dot-blot” postupkom, a određivanje terminalne galaktoze lektinskim imunoblot postupkom. Rezultati: Raspodela IgG podklasa u obe tečnosti se razlikovala kod parodontopatija i u kontrolnim uzorcima. I u pljuvačci i u gingivalnoj tečnosti obolelih, kvantitativno je dominirala IgG2 podklasa, nezavisno od parodontalnog statusa. U IgG obe oralne tečnosti terminalna galaktoza je bila eksprimirana kod osoba sa zdravim parodoncijumom (kontrola) i kod osoba sa početnom (inicijalnom) parodontopatijom, dok kod osoba sa uznapredovalom parodontopatijom ekspresija ovog šećera nije registrovana ni u jednoj od ove dve tečnosti. Zaključak: Rezultati ovih istraživanja ukazuju da postoji pomeranje prema hipogalaktozilovanim IgG glikoformama tokom procesa inflamacije gingive u obolelih od parodontopatije.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease
T1  - Hipogalaktozilacija imunoglobulina G (IgG) gingivalne tečnosti i pljuvačke u obolelih od parodontopatije
VL  - 53
IS  - 1
SP  - 7
EP  - 16
DO  - 10.2298/SGS0601007S
ER  - 

@article{
author = "Stefanović, Gordana and Ćirić, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević, Dragan and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Changed glycosylation of immunoglobulin G (IgG), above all, the expression of thermal galactose, influence to numerous functions of those immunoglobulin and correlate with the inflammatory level in a number of diseases. Aim: This work analyses the distribution of IgG subclasses and the content of thermal galactose in them, in saliva and gingival fluid of the patients with periodontal disease and different gum inflammatory level. Materials and methods: It was used saliva and gingival fluid of 30 adults with clinical picture of periodontal disease and 20 persons with healthy periodontium. The qualification of IgG was done by “dot-blot” procedure and the, and thermal galactose was determined by lectin immunoblot procedure. Results: The division of IgG subclasses in both fluids was different in the patients with periodontal disease and in control samples. In saliva and gingival fluid of the diseased quantitatively dominated IgG2 subclasses, independently from periodontal status. In IgG of both fluids, thermal galactose was exprimated at the healthy periodontium persons (control) and with the person with initial periodontal disease, while at the person with increased periodontal disease the expression of this saccharide wasn’t registered in neither of fluids. Conclusion: The results showed that there is a shift towards hypogalactosyled IgG glikoforms during the process of gum inflammation at the periodontal disease patients., Promenjena glikozilacija imunoglobulina G (IgG), pre svega ekspresija terminalne galaktoze, utiče na brojne funkcije ovih imunoglobulina i korelira sa stepenom zapaljenja u mnogim bolestima. Cilj rada: U ovom radu analizirana je distribucija IgG podklasa i sadržaj terminalne galaktoze u njima, u pljuvačci i gingivalnoj tečnosti bolesnika sa parodontopatijom različitog stepena inflamacije gingive. Materijal i metod: Kao materijal u ispitivanjima korišćena je gingivalna tečnost i pljuvačka 30 odraslih osoba sa kliničkom dijagnozom parodontopatija i 20 osoba sa zdravim parodoncijumom. Kvantifikacija IgG urađena je “dot-blot” postupkom, a određivanje terminalne galaktoze lektinskim imunoblot postupkom. Rezultati: Raspodela IgG podklasa u obe tečnosti se razlikovala kod parodontopatija i u kontrolnim uzorcima. I u pljuvačci i u gingivalnoj tečnosti obolelih, kvantitativno je dominirala IgG2 podklasa, nezavisno od parodontalnog statusa. U IgG obe oralne tečnosti terminalna galaktoza je bila eksprimirana kod osoba sa zdravim parodoncijumom (kontrola) i kod osoba sa početnom (inicijalnom) parodontopatijom, dok kod osoba sa uznapredovalom parodontopatijom ekspresija ovog šećera nije registrovana ni u jednoj od ove dve tečnosti. Zaključak: Rezultati ovih istraživanja ukazuju da postoji pomeranje prema hipogalaktozilovanim IgG glikoformama tokom procesa inflamacije gingive u obolelih od parodontopatije.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease, Hipogalaktozilacija imunoglobulina G (IgG) gingivalne tečnosti i pljuvačke u obolelih od parodontopatije",
volume = "53",
number = "1",
pages = "7-16",
doi = "10.2298/SGS0601007S"
}

Stefanović, G., Ćirić, D., Ilić, V., Brajović, G., Petrović, S., Milošević, D.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 53(1), 7-16.
https://doi.org/10.2298/SGS0601007S

Stefanović G, Ćirić D, Ilić V, Brajović G, Petrović S, Milošević D, Milošević-Jovčić N. Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease. in Stomatološki glasnik Srbije. 2006;53(1):7-16.
doi:10.2298/SGS0601007S .

Stefanović, Gordana, Ćirić, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević, Dragan, Milošević-Jovčić, Nadežda, "Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease" in Stomatološki glasnik Srbije, 53, no. 1 (2006):7-16,
https://doi.org/10.2298/SGS0601007S . .