TY  - JOUR
AU  - Popović, Branka
AU  - Velimirović, Milica
AU  - Stojković, Tihomir
AU  - Brajović, Gavrilo
AU  - de Luka, Silvio R.
AU  - Milovanović, Ivan
AU  - Stefanović, Srđan
AU  - Nikolić, Dragica
AU  - Ristic-Đurović, Jasna L.
AU  - Petronijević, Nataša
AU  - Trbovich, Alexander M.
PY  - 2018
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2323
AB  - Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - The influence of ageing on the extrapineal melatonin synthetic pathway
VL  - 110
SP  - 151
EP  - 157
DO  - 10.1016/j.exger.2018.06.010
ER  - 

@article{
author = "Popović, Branka and Velimirović, Milica and Stojković, Tihomir and Brajović, Gavrilo and de Luka, Silvio R. and Milovanović, Ivan and Stefanović, Srđan and Nikolić, Dragica and Ristic-Đurović, Jasna L. and Petronijević, Nataša and Trbovich, Alexander M.",
year = "2018",
abstract = "Ageing affects various physiological and metabolic processes in a body and a progressive accumulation of oxidative damage stands out as often used explanation. One of the most powerful scavenger of reactive oxygen species (ROS) in all organs is melatonin. A majority of melatonin supplied to the body via blood originates from the pineal gland. However, we have been interested in a locally produced melatonin. We have used 2.5- and 36-months-old Wistar rats. Tissues were collected and gene expression of AA-NAT and ASMT, the two key enzymes in a synthesis of melatonin, was determined in brain, liver, kidney, heart, skin, and intestine. Since melatonin can influence antioxidant enzymes, the activity of superoxide dismutase (SOD) and catalase (CAT), and the level of GSH were measured in liver. In addition, Copper (Cu), Zinc (Zn), and Manganese (Mn) were also determined in liver since these microelements might affect the activity of antioxidant enzymes. The expression of AA-NAT and ASMT was increased in liver and skin of old animals. A positive correlation in AA-NAT and ASMT expression was observed in liver, intestine and kidney. Moreover, the activity of CAT enzyme in liver was increased while SOD activity was decreased. SOD and CAT were probably affected by the observed decreased amount of Cu, Zn, and Mn in liver of old animals. In our model, extrapineal melatonin pathway in ageing consisted of complex interplay of locally produced melatonin, activities of SOD and CAT, and adequate presence of Cu, Zn and Mn microelements in order to defend organs against oxidative damage.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "The influence of ageing on the extrapineal melatonin synthetic pathway",
volume = "110",
pages = "151-157",
doi = "10.1016/j.exger.2018.06.010"
}

Popović, B., Velimirović, M., Stojković, T., Brajović, G., de Luka, S. R., Milovanović, I., Stefanović, S., Nikolić, D., Ristic-Đurović, J. L., Petronijević, N.,& Trbovich, A. M.. (2018). The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 110, 151-157.
https://doi.org/10.1016/j.exger.2018.06.010

Popović B, Velimirović M, Stojković T, Brajović G, de Luka SR, Milovanović I, Stefanović S, Nikolić D, Ristic-Đurović JL, Petronijević N, Trbovich AM. The influence of ageing on the extrapineal melatonin synthetic pathway. in Experimental Gerontology. 2018;110:151-157.
doi:10.1016/j.exger.2018.06.010 .

Popović, Branka, Velimirović, Milica, Stojković, Tihomir, Brajović, Gavrilo, de Luka, Silvio R., Milovanović, Ivan, Stefanović, Srđan, Nikolić, Dragica, Ristic-Đurović, Jasna L., Petronijević, Nataša, Trbovich, Alexander M., "The influence of ageing on the extrapineal melatonin synthetic pathway" in Experimental Gerontology, 110 (2018):151-157,
https://doi.org/10.1016/j.exger.2018.06.010 . .

TY  - JOUR
AU  - Čarkić, Jelena
AU  - Nikolić, Nadja
AU  - Radojević-Škodrić, Sanja
AU  - Kuzmanović-Pfićer, Jovana
AU  - Brajović, Gavrilo
AU  - Antunović, Marija
AU  - Milašin, Jelena
AU  - Popović, Branka
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2115
AB  - The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.
PB  - Nihon Univ, School Dentistry, Toyko
T2  - Journal of Oral Science
T1  - The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma
VL  - 58
IS  - 4
SP  - 449
EP  - 458
DO  - 10.2334/josnusd.16-0108
ER  - 

@article{
author = "Čarkić, Jelena and Nikolić, Nadja and Radojević-Škodrić, Sanja and Kuzmanović-Pfićer, Jovana and Brajović, Gavrilo and Antunović, Marija and Milašin, Jelena and Popović, Branka",
year = "2016",
abstract = "The aim of this study was to assess TERT-CLPTM1L single-nucleotide polymorphisms (SNPs) (rs402710 C/T in the CLPTM1L gene; rs2736100 A/C and rs2736098 G/A in the TERT gene) as risk factors for development of oral squamous cell carcinoma (OSCC), and to investigate the relationship between the analyzed polymorphisms, relative telomere length (RTL), telomerase expression and clinicopathologic characteristics of OSCC in a Serbian population. Paraffin-embedded tumor samples and buccal swabs from cancer-free controls were genotyped using PCR-RFLP, while tumor RTL values and telomerase expression were estimated by real-time PCR and immunohistochemistry, respectively. CLPTM1L rs402710 and TERT rs2736100 polymorphisms were associated with a significantly increased risk of OSCC, and TERT rs2736098 with a significantly decreased risk. No significant association was found between TERT-CLPTM1L polymorphisms, tumor RTL values, telomerase expression, and clinicopathologic features, although a trend towards longer telomeres was evident in telomerase-positive samples and less advanced tumors. Kaplan-Meier survival analysis showed that patients with longer telomeres in their tumors had significantly better overall survival than patients with shorter telomeres. Our research seems to provide strong evidence for an association between CLPTMIL rs402710C/T and TERT rs2736100A/C SNPs and the risk of OSSC, and suggests that higher tumor RTL values and positive hTERT expression may be applicable as early prognostic markers.",
publisher = "Nihon Univ, School Dentistry, Toyko",
journal = "Journal of Oral Science",
title = "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma",
volume = "58",
number = "4",
pages = "449-458",
doi = "10.2334/josnusd.16-0108"
}

Čarkić, J., Nikolić, N., Radojević-Škodrić, S., Kuzmanović-Pfićer, J., Brajović, G., Antunović, M., Milašin, J.,& Popović, B.. (2016). The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science
Nihon Univ, School Dentistry, Toyko., 58(4), 449-458.
https://doi.org/10.2334/josnusd.16-0108

Čarkić J, Nikolić N, Radojević-Škodrić S, Kuzmanović-Pfićer J, Brajović G, Antunović M, Milašin J, Popović B. The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma. in Journal of Oral Science. 2016;58(4):449-458.
doi:10.2334/josnusd.16-0108 .

Čarkić, Jelena, Nikolić, Nadja, Radojević-Škodrić, Sanja, Kuzmanović-Pfićer, Jovana, Brajović, Gavrilo, Antunović, Marija, Milašin, Jelena, Popović, Branka, "The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma" in Journal of Oral Science, 58, no. 4 (2016):449-458,
https://doi.org/10.2334/josnusd.16-0108 . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Popović, Branka
AU  - Puletić, Miljan
AU  - Kostić, Marija
AU  - Milašin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2155
AB  - Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.
AB  - Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Estimation of total bacteria by real-time PCR in patients with periodontal disease
T1  - Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom
VL  - 144
IS  - 1-2
SP  - 10
EP  - 14
DO  - 10.2298/SARH1602010B
ER  - 

@article{
author = "Brajović, Gavrilo and Popović, Branka and Puletić, Miljan and Kostić, Marija and Milašin, Jelena",
year = "2016",
abstract = "Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis., Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Estimation of total bacteria by real-time PCR in patients with periodontal disease, Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom",
volume = "144",
number = "1-2",
pages = "10-14",
doi = "10.2298/SARH1602010B"
}

Brajović, G., Popović, B., Puletić, M., Kostić, M.,& Milašin, J.. (2016). Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(1-2), 10-14.
https://doi.org/10.2298/SARH1602010B

Brajović G, Popović B, Puletić M, Kostić M, Milašin J. Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo. 2016;144(1-2):10-14.
doi:10.2298/SARH1602010B .

Brajović, Gavrilo, Popović, Branka, Puletić, Miljan, Kostić, Marija, Milašin, Jelena, "Estimation of total bacteria by real-time PCR in patients with periodontal disease" in Srpski arhiv za celokupno lekarstvo, 144, no. 1-2 (2016):10-14,
https://doi.org/10.2298/SARH1602010B . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Ilić, Vesna
AU  - Mojsilović, Sonja
AU  - Marković, Dragana
AU  - Milošević-Jovčić, Nadežda
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2046
AB  - Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects.
AB  - Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease
T1  - Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije
VL  - 62
IS  - 2
SP  - 55
EP  - 64
DO  - 10.1515/sdj-2015-0006
ER  - 

@article{
author = "Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Ilić, Vesna and Mojsilović, Sonja and Marković, Dragana and Milošević-Jovčić, Nadežda",
year = "2015",
abstract = "Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects., Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease, Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije",
volume = "62",
number = "2",
pages = "55-64",
doi = "10.1515/sdj-2015-0006"
}

Brajović, G., Nikolić-Jakoba, N., Popović, B., Ilić, V., Mojsilović, S., Marković, D.,& Milošević-Jovčić, N.. (2015). Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(2), 55-64.
https://doi.org/10.1515/sdj-2015-0006

Brajović G, Nikolić-Jakoba N, Popović B, Ilić V, Mojsilović S, Marković D, Milošević-Jovčić N. Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije. 2015;62(2):55-64.
doi:10.1515/sdj-2015-0006 .

Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Ilić, Vesna, Mojsilović, Sonja, Marković, Dragana, Milošević-Jovčić, Nadežda, "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease" in Stomatološki glasnik Srbije, 62, no. 2 (2015):55-64,
https://doi.org/10.1515/sdj-2015-0006 . .

TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Stefanović, Gordana
AU  - Ilić, Vesna
AU  - Petrović, Sonja
AU  - Stefanović, Neda
AU  - Nikolić-Jakoba, Nataša
AU  - Milošević-Jovčić, Nadežda
PY  - 2010
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1563
AB  - Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.
PB  - Wiley, Hoboken
T2  - Journal of Periodontology
T1  - Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis
VL  - 81
IS  - 10
SP  - 1472
EP  - 1480
DO  - 10.1902/jop.2010.100053
ER  - 

@article{
author = "Brajović, Gavrilo and Stefanović, Gordana and Ilić, Vesna and Petrović, Sonja and Stefanović, Neda and Nikolić-Jakoba, Nataša and Milošević-Jovčić, Nadežda",
year = "2010",
abstract = "Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48- to 53-kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains. J Periodontol 2010;81:1472-1480.",
publisher = "Wiley, Hoboken",
journal = "Journal of Periodontology",
title = "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis",
volume = "81",
number = "10",
pages = "1472-1480",
doi = "10.1902/jop.2010.100053"
}

Brajović, G., Stefanović, G., Ilić, V., Petrović, S., Stefanović, N., Nikolić-Jakoba, N.,& Milošević-Jovčić, N.. (2010). Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology
Wiley, Hoboken., 81(10), 1472-1480.
https://doi.org/10.1902/jop.2010.100053

Brajović G, Stefanović G, Ilić V, Petrović S, Stefanović N, Nikolić-Jakoba N, Milošević-Jovčić N. Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis. in Journal of Periodontology. 2010;81(10):1472-1480.
doi:10.1902/jop.2010.100053 .

Brajović, Gavrilo, Stefanović, Gordana, Ilić, Vesna, Petrović, Sonja, Stefanović, Neda, Nikolić-Jakoba, Nataša, Milošević-Jovčić, Nadežda, "Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis" in Journal of Periodontology, 81, no. 10 (2010):1472-1480,
https://doi.org/10.1902/jop.2010.100053 . .

TY  - JOUR
AU  - Milićević, Radovan
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Pavlica, Dušan
AU  - Leković, Vojislav
AU  - Milašin, Jelena
PY  - 2008
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1385
AB  - INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.
AB  - Uvod Epidemiološki podaci iz čitavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma ključnu ulogu igraju različiti rodovi Gram-negativnih bakterija, ponajviše striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u različitim uzorcima poreklom iz usne duplje pacijenata s klinički dijagnostikovanom parodontopatijom. Metod rada Kao biološki materijal u kojem je dokazivano postojanje DNK mikroorganizama korišćeni su zubni plak, tkivo zapaljene gingive i pljuvačka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lančanog umnožavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve različite bakterije. Rezultati S manjom ili većom učestalošću, u svim ispitanim uzorcima utvrđeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najčešći je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najvećem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, što je odlika hroničnog oblika parodontopatije, a u pljuvački ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapaženo u pljuvački. Zaključak Primenjeni metod PCR ima veliku osetljivost i specifičnost. Brzo i precizno otkrivanje mikroorganizama je veoma važno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i lečenje parodontopatija. U svakodnevnoj kliničkoj praksi optimalan biološki materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Identification of periodontopathogen microorganisms by PCR technique
T1  - Identifikacija parodontopatogenih mikroorganizama PCR tehnikom
VL  - 136
IS  - 9-10
SP  - 476
EP  - 480
DO  - 10.2298/SARH0810476M
ER  - 

@article{
author = "Milićević, Radovan and Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Pavlica, Dušan and Leković, Vojislav and Milašin, Jelena",
year = "2008",
abstract = "INTRODUCTION Periodontitis is an inflammatory disease of the supporting tissues of teeth and is a major cause of tooth loss in adults. The onset and progression of periodontal disease is attributed to the presence of elevated levels of a consortium of pathogenic bacteria. Gram negative bacteria, mainly strict anaerobes, play the major role. OBJECTIVE The present study aimed to assess the presence of the main types of microorganisms involved in the aetiopathogenesis of periodontal disease: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia and Prevotella intermedia in different samples collected from the oral cavity of 90 patients diagnosed with periodontitis. METHOD Bacterial DNA detection was performed in diverse biological materials, namely in dental plaque, gingival tissue and saliva, by means of multiplex PCR, a technique that allows simultaneous identification of two different bacterial genomes. RESULTS In the dental plaque of the periodontitis patients, Treponema denticola dominated. In the gingival tissue, Tannerella forsythia and Treponema denticola were the microbiota most frequently detected, whilst in saliva Treponema denticola and Eikenella corrodens were found with the highest percentage. CONCLUSION The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria., Uvod Epidemiološki podaci iz čitavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma ključnu ulogu igraju različiti rodovi Gram-negativnih bakterija, ponajviše striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u različitim uzorcima poreklom iz usne duplje pacijenata s klinički dijagnostikovanom parodontopatijom. Metod rada Kao biološki materijal u kojem je dokazivano postojanje DNK mikroorganizama korišćeni su zubni plak, tkivo zapaljene gingive i pljuvačka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lančanog umnožavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve različite bakterije. Rezultati S manjom ili većom učestalošću, u svim ispitanim uzorcima utvrđeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najčešći je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najvećem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, što je odlika hroničnog oblika parodontopatije, a u pljuvački ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapaženo u pljuvački. Zaključak Primenjeni metod PCR ima veliku osetljivost i specifičnost. Brzo i precizno otkrivanje mikroorganizama je veoma važno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i lečenje parodontopatija. U svakodnevnoj kliničkoj praksi optimalan biološki materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Identification of periodontopathogen microorganisms by PCR technique, Identifikacija parodontopatogenih mikroorganizama PCR tehnikom",
volume = "136",
number = "9-10",
pages = "476-480",
doi = "10.2298/SARH0810476M"
}

Milićević, R., Brajović, G., Nikolić-Jakoba, N., Popović, B., Pavlica, D., Leković, V.,& Milašin, J.. (2008). Identification of periodontopathogen microorganisms by PCR technique. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 136(9-10), 476-480.
https://doi.org/10.2298/SARH0810476M

Milićević R, Brajović G, Nikolić-Jakoba N, Popović B, Pavlica D, Leković V, Milašin J. Identification of periodontopathogen microorganisms by PCR technique. in Srpski arhiv za celokupno lekarstvo. 2008;136(9-10):476-480.
doi:10.2298/SARH0810476M .

Milićević, Radovan, Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Pavlica, Dušan, Leković, Vojislav, Milašin, Jelena, "Identification of periodontopathogen microorganisms by PCR technique" in Srpski arhiv za celokupno lekarstvo, 136, no. 9-10 (2008):476-480,
https://doi.org/10.2298/SARH0810476M . .

TY  - JOUR
AU  - Stefanović, Gordana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1318
AB  - Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.
PB  - Amer Acad Periodontology, Chicago
T2  - Journal of Periodontology
T1  - Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis
VL  - 77
IS  - 11
SP  - 1887
EP  - 1893
DO  - 10.1902/jop.2006.060049
ER  - 

@article{
author = "Stefanović, Gordana and Marković, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.",
publisher = "Amer Acad Periodontology, Chicago",
journal = "Journal of Periodontology",
title = "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis",
volume = "77",
number = "11",
pages = "1887-1893",
doi = "10.1902/jop.2006.060049"
}

Stefanović, G., Marković, D., Ilić, V., Brajović, G., Petrović, S.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology
Amer Acad Periodontology, Chicago., 77(11), 1887-1893.
https://doi.org/10.1902/jop.2006.060049

Stefanović G, Marković D, Ilić V, Brajović G, Petrović S, Milošević-Jovčić N. Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology. 2006;77(11):1887-1893.
doi:10.1902/jop.2006.060049 .

Stefanović, Gordana, Marković, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević-Jovčić, Nadežda, "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis" in Journal of Periodontology, 77, no. 11 (2006):1887-1893,
https://doi.org/10.1902/jop.2006.060049 . .

TY  - JOUR
AU  - Stefanović, Gordana
AU  - Ćirić, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević, Dragan
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1310
AB  - Changed glycosylation of immunoglobulin G (IgG), above all, the expression of thermal galactose, influence to numerous functions of those immunoglobulin and correlate with the inflammatory level in a number of diseases. Aim: This work analyses the distribution of IgG subclasses and the content of thermal galactose in them, in saliva and gingival fluid of the patients with periodontal disease and different gum inflammatory level. Materials and methods: It was used saliva and gingival fluid of 30 adults with clinical picture of periodontal disease and 20 persons with healthy periodontium. The qualification of IgG was done by “dot-blot” procedure and the, and thermal galactose was determined by lectin immunoblot procedure. Results: The division of IgG subclasses in both fluids was different in the patients with periodontal disease and in control samples. In saliva and gingival fluid of the diseased quantitatively dominated IgG2 subclasses, independently from periodontal status. In IgG of both fluids, thermal galactose was exprimated at the healthy periodontium persons (control) and with the person with initial periodontal disease, while at the person with increased periodontal disease the expression of this saccharide wasn’t registered in neither of fluids. Conclusion: The results showed that there is a shift towards hypogalactosyled IgG glikoforms during the process of gum inflammation at the periodontal disease patients.
AB  - Promenjena glikozilacija imunoglobulina G (IgG), pre svega ekspresija terminalne galaktoze, utiče na brojne funkcije ovih imunoglobulina i korelira sa stepenom zapaljenja u mnogim bolestima. Cilj rada: U ovom radu analizirana je distribucija IgG podklasa i sadržaj terminalne galaktoze u njima, u pljuvačci i gingivalnoj tečnosti bolesnika sa parodontopatijom različitog stepena inflamacije gingive. Materijal i metod: Kao materijal u ispitivanjima korišćena je gingivalna tečnost i pljuvačka 30 odraslih osoba sa kliničkom dijagnozom parodontopatija i 20 osoba sa zdravim parodoncijumom. Kvantifikacija IgG urađena je “dot-blot” postupkom, a određivanje terminalne galaktoze lektinskim imunoblot postupkom. Rezultati: Raspodela IgG podklasa u obe tečnosti se razlikovala kod parodontopatija i u kontrolnim uzorcima. I u pljuvačci i u gingivalnoj tečnosti obolelih, kvantitativno je dominirala IgG2 podklasa, nezavisno od parodontalnog statusa. U IgG obe oralne tečnosti terminalna galaktoza je bila eksprimirana kod osoba sa zdravim parodoncijumom (kontrola) i kod osoba sa početnom (inicijalnom) parodontopatijom, dok kod osoba sa uznapredovalom parodontopatijom ekspresija ovog šećera nije registrovana ni u jednoj od ove dve tečnosti. Zaključak: Rezultati ovih istraživanja ukazuju da postoji pomeranje prema hipogalaktozilovanim IgG glikoformama tokom procesa inflamacije gingive u obolelih od parodontopatije.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease
T1  - Hipogalaktozilacija imunoglobulina G (IgG) gingivalne tečnosti i pljuvačke u obolelih od parodontopatije
VL  - 53
IS  - 1
SP  - 7
EP  - 16
DO  - 10.2298/SGS0601007S
ER  - 

@article{
author = "Stefanović, Gordana and Ćirić, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević, Dragan and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Changed glycosylation of immunoglobulin G (IgG), above all, the expression of thermal galactose, influence to numerous functions of those immunoglobulin and correlate with the inflammatory level in a number of diseases. Aim: This work analyses the distribution of IgG subclasses and the content of thermal galactose in them, in saliva and gingival fluid of the patients with periodontal disease and different gum inflammatory level. Materials and methods: It was used saliva and gingival fluid of 30 adults with clinical picture of periodontal disease and 20 persons with healthy periodontium. The qualification of IgG was done by “dot-blot” procedure and the, and thermal galactose was determined by lectin immunoblot procedure. Results: The division of IgG subclasses in both fluids was different in the patients with periodontal disease and in control samples. In saliva and gingival fluid of the diseased quantitatively dominated IgG2 subclasses, independently from periodontal status. In IgG of both fluids, thermal galactose was exprimated at the healthy periodontium persons (control) and with the person with initial periodontal disease, while at the person with increased periodontal disease the expression of this saccharide wasn’t registered in neither of fluids. Conclusion: The results showed that there is a shift towards hypogalactosyled IgG glikoforms during the process of gum inflammation at the periodontal disease patients., Promenjena glikozilacija imunoglobulina G (IgG), pre svega ekspresija terminalne galaktoze, utiče na brojne funkcije ovih imunoglobulina i korelira sa stepenom zapaljenja u mnogim bolestima. Cilj rada: U ovom radu analizirana je distribucija IgG podklasa i sadržaj terminalne galaktoze u njima, u pljuvačci i gingivalnoj tečnosti bolesnika sa parodontopatijom različitog stepena inflamacije gingive. Materijal i metod: Kao materijal u ispitivanjima korišćena je gingivalna tečnost i pljuvačka 30 odraslih osoba sa kliničkom dijagnozom parodontopatija i 20 osoba sa zdravim parodoncijumom. Kvantifikacija IgG urađena je “dot-blot” postupkom, a određivanje terminalne galaktoze lektinskim imunoblot postupkom. Rezultati: Raspodela IgG podklasa u obe tečnosti se razlikovala kod parodontopatija i u kontrolnim uzorcima. I u pljuvačci i u gingivalnoj tečnosti obolelih, kvantitativno je dominirala IgG2 podklasa, nezavisno od parodontalnog statusa. U IgG obe oralne tečnosti terminalna galaktoza je bila eksprimirana kod osoba sa zdravim parodoncijumom (kontrola) i kod osoba sa početnom (inicijalnom) parodontopatijom, dok kod osoba sa uznapredovalom parodontopatijom ekspresija ovog šećera nije registrovana ni u jednoj od ove dve tečnosti. Zaključak: Rezultati ovih istraživanja ukazuju da postoji pomeranje prema hipogalaktozilovanim IgG glikoformama tokom procesa inflamacije gingive u obolelih od parodontopatije.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease, Hipogalaktozilacija imunoglobulina G (IgG) gingivalne tečnosti i pljuvačke u obolelih od parodontopatije",
volume = "53",
number = "1",
pages = "7-16",
doi = "10.2298/SGS0601007S"
}

Stefanović, G., Ćirić, D., Ilić, V., Brajović, G., Petrović, S., Milošević, D.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 53(1), 7-16.
https://doi.org/10.2298/SGS0601007S

Stefanović G, Ćirić D, Ilić V, Brajović G, Petrović S, Milošević D, Milošević-Jovčić N. Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease. in Stomatološki glasnik Srbije. 2006;53(1):7-16.
doi:10.2298/SGS0601007S .

Stefanović, Gordana, Ćirić, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević, Dragan, Milošević-Jovčić, Nadežda, "Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease" in Stomatološki glasnik Srbije, 53, no. 1 (2006):7-16,
https://doi.org/10.2298/SGS0601007S . .

TY  - JOUR
AU  - Todorović, Tatjana
AU  - Dožić, Ivan
AU  - Pavlica, Dušan
AU  - Marković, Dejan
AU  - Ivanović, Mirjana
AU  - Brajović, Gavrilo
AU  - Stefanović, Gordana
AU  - Mirković, Silvija
AU  - Anđelski, Biljana
PY  - 2005
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1233
AB  - Saliva is a secretion of the salivary and mucous glands and is of major importance in the maintainance of oral health. Over the last few decades, saliva has been evaluated as a diagnostic fluid in medicine for determining systemic disease markers as well as for monitoring numerous drugs, narcotics, and hormones. The biochemical analysis of saliva is particularly important in dentistry. The estimation of the risk of appearance and diagnosis of disease, monitoring of disease progression, evaluation of therapy efficacy for caries, periodontitis, premalignant and malignant oral lesions, as well as infectious diseases of the oral cavity, can be assessed by analyzing different constituent: of saliva, individuals at risk of caries can be identified using test: that determine saliva flow rate, saliva buffer capacity, and colonization of the oral cavity by cariogenic bacteria. Today, these rapid and simple diagnostic tests are used routinely in caries risk determination. The study and use of saliva-based diagnostics have increased over the last few decades. Clinical testing of saliva shows much promise. However, there is a need for much additional research in this area, before the true clinical value of saliva as a diagnostic fluid in dentistry can be determined.
AB  - Pljuvačka je sekret pljuvačnih i mukoznih žlezda koji je značajan za održavanje oralnog zdravlja. Poslednjih decenija sve više se razmatra mogućnost njene primene kao zamena krvi, odnosno krvnom serumu i plazme, radi dokazivanja pokazatelja sistemskih oboljenja, kao i za nadgledanje primene lekova, droga i hormona. Analiziranje biohemijskog sastava pljuvačke posebno je značajno u stomatologiji za procenu rizika nastanka, praćenja toka bolesti i kontrole rezultata primenjenog lečenja zubnog karijesa, parodontopatije, oralnih karcinoma i infektivnih oboljenja usne duplje. U proceni rizika nastanka karijesa na zubima rutinski se izvode ispitivanja kojima se određuju količina izlučene pljuvačke, njen puferski kapacitet i stepen kolonizacije usne duplje kariogenim vrstama bakterija. Jednostavnost izvođenja ovih ispitivanja omogućava da ih u stomatološkoj ordinaciji primenjuje lekar praktičar, što predstavlja veliku pomoć u proceni rizika za nastanak zubnog karijesa. Zbog bliskog kontakta s oralnim tkivima, pljuvačka sadrži brojne biohemijske pokazatelje patoloških procesa lokalizovanih u ovim tkivima (enzimi, imunoglobulini, ostali proteini, fenotipski pokazatelji). Ispitivanje pomenutih pokazatelja je značajno kao dopuna kliničkom dijagnostikovanju ukoliko ono ne pruža dovoljno informacija.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Use of saliva as a diagnostic fluid in dentistry
T1  - Mogućnosti upotrebe pljuvačke kao dijagnostičke tečnosti u stomatologiji
VL  - 133
IS  - 7-8
SP  - 372
EP  - 378
DO  - 10.2298/SARH0508372T
ER  - 

@article{
author = "Todorović, Tatjana and Dožić, Ivan and Pavlica, Dušan and Marković, Dejan and Ivanović, Mirjana and Brajović, Gavrilo and Stefanović, Gordana and Mirković, Silvija and Anđelski, Biljana",
year = "2005",
abstract = "Saliva is a secretion of the salivary and mucous glands and is of major importance in the maintainance of oral health. Over the last few decades, saliva has been evaluated as a diagnostic fluid in medicine for determining systemic disease markers as well as for monitoring numerous drugs, narcotics, and hormones. The biochemical analysis of saliva is particularly important in dentistry. The estimation of the risk of appearance and diagnosis of disease, monitoring of disease progression, evaluation of therapy efficacy for caries, periodontitis, premalignant and malignant oral lesions, as well as infectious diseases of the oral cavity, can be assessed by analyzing different constituent: of saliva, individuals at risk of caries can be identified using test: that determine saliva flow rate, saliva buffer capacity, and colonization of the oral cavity by cariogenic bacteria. Today, these rapid and simple diagnostic tests are used routinely in caries risk determination. The study and use of saliva-based diagnostics have increased over the last few decades. Clinical testing of saliva shows much promise. However, there is a need for much additional research in this area, before the true clinical value of saliva as a diagnostic fluid in dentistry can be determined., Pljuvačka je sekret pljuvačnih i mukoznih žlezda koji je značajan za održavanje oralnog zdravlja. Poslednjih decenija sve više se razmatra mogućnost njene primene kao zamena krvi, odnosno krvnom serumu i plazme, radi dokazivanja pokazatelja sistemskih oboljenja, kao i za nadgledanje primene lekova, droga i hormona. Analiziranje biohemijskog sastava pljuvačke posebno je značajno u stomatologiji za procenu rizika nastanka, praćenja toka bolesti i kontrole rezultata primenjenog lečenja zubnog karijesa, parodontopatije, oralnih karcinoma i infektivnih oboljenja usne duplje. U proceni rizika nastanka karijesa na zubima rutinski se izvode ispitivanja kojima se određuju količina izlučene pljuvačke, njen puferski kapacitet i stepen kolonizacije usne duplje kariogenim vrstama bakterija. Jednostavnost izvođenja ovih ispitivanja omogućava da ih u stomatološkoj ordinaciji primenjuje lekar praktičar, što predstavlja veliku pomoć u proceni rizika za nastanak zubnog karijesa. Zbog bliskog kontakta s oralnim tkivima, pljuvačka sadrži brojne biohemijske pokazatelje patoloških procesa lokalizovanih u ovim tkivima (enzimi, imunoglobulini, ostali proteini, fenotipski pokazatelji). Ispitivanje pomenutih pokazatelja je značajno kao dopuna kliničkom dijagnostikovanju ukoliko ono ne pruža dovoljno informacija.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Use of saliva as a diagnostic fluid in dentistry, Mogućnosti upotrebe pljuvačke kao dijagnostičke tečnosti u stomatologiji",
volume = "133",
number = "7-8",
pages = "372-378",
doi = "10.2298/SARH0508372T"
}

Todorović, T., Dožić, I., Pavlica, D., Marković, D., Ivanović, M., Brajović, G., Stefanović, G., Mirković, S.,& Anđelski, B.. (2005). Use of saliva as a diagnostic fluid in dentistry. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 133(7-8), 372-378.
https://doi.org/10.2298/SARH0508372T

Todorović T, Dožić I, Pavlica D, Marković D, Ivanović M, Brajović G, Stefanović G, Mirković S, Anđelski B. Use of saliva as a diagnostic fluid in dentistry. in Srpski arhiv za celokupno lekarstvo. 2005;133(7-8):372-378.
doi:10.2298/SARH0508372T .

Todorović, Tatjana, Dožić, Ivan, Pavlica, Dušan, Marković, Dejan, Ivanović, Mirjana, Brajović, Gavrilo, Stefanović, Gordana, Mirković, Silvija, Anđelski, Biljana, "Use of saliva as a diagnostic fluid in dentistry" in Srpski arhiv za celokupno lekarstvo, 133, no. 7-8 (2005):372-378,
https://doi.org/10.2298/SARH0508372T . .

TY  - JOUR
AU  - Brajović, Gavrilo
PY  - 2004
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1212
AB  - The autotomy phenomenon is an animal model that is used for studying chronic pain. It is based on the observation that transection of peripheral nerve in animals triggers self-mutilation of denervated limb. Autotomy phenomenon today presents the most reliable and useful tool for experimental investigation of pain-suppressing effect of some substances on experimentally induced pain. Organic alcohol glycerol has been used for peripheral blocks in treating paroxysmal trigeminal neuralgia. There is evidence that sorbitol, also an organic alcohol, disturbs nerve function in humans. Therefore, the present study was aimed to investigate the effects of 2 organic alcohols on autotomy phenomenon after peripheral nerve lesions. After inducing autotomy, 12 rats were divided into 3 groups, each comprising 4 animals, according to the substance applied in order to suppress autotomy (sorbitol solution in the 1st, absolute glycerol in the 2nd, and saline in the 3rd, the control group). Degree of self-mutilation was measured for 8 weeks. Results indicated the efficacy of glycerol and sorbitol in relieving experimentally induced pain.
PB  - Udruženje stomatologa Balkana
T2  - Balkan Journal of Stomatology
T1  - The effects of sorbitol on autotomy in rats after peripheral nerve lesions
VL  - 8
IS  - 2
SP  - 109
EP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_smile_1212
ER  - 

@article{
author = "Brajović, Gavrilo",
year = "2004",
abstract = "The autotomy phenomenon is an animal model that is used for studying chronic pain. It is based on the observation that transection of peripheral nerve in animals triggers self-mutilation of denervated limb. Autotomy phenomenon today presents the most reliable and useful tool for experimental investigation of pain-suppressing effect of some substances on experimentally induced pain. Organic alcohol glycerol has been used for peripheral blocks in treating paroxysmal trigeminal neuralgia. There is evidence that sorbitol, also an organic alcohol, disturbs nerve function in humans. Therefore, the present study was aimed to investigate the effects of 2 organic alcohols on autotomy phenomenon after peripheral nerve lesions. After inducing autotomy, 12 rats were divided into 3 groups, each comprising 4 animals, according to the substance applied in order to suppress autotomy (sorbitol solution in the 1st, absolute glycerol in the 2nd, and saline in the 3rd, the control group). Degree of self-mutilation was measured for 8 weeks. Results indicated the efficacy of glycerol and sorbitol in relieving experimentally induced pain.",
publisher = "Udruženje stomatologa Balkana",
journal = "Balkan Journal of Stomatology",
title = "The effects of sorbitol on autotomy in rats after peripheral nerve lesions",
volume = "8",
number = "2",
pages = "109-113",
url = "https://hdl.handle.net/21.15107/rcub_smile_1212"
}

Brajović, G.. (2004). The effects of sorbitol on autotomy in rats after peripheral nerve lesions. in Balkan Journal of Stomatology
Udruženje stomatologa Balkana., 8(2), 109-113.
https://hdl.handle.net/21.15107/rcub_smile_1212

Brajović G. The effects of sorbitol on autotomy in rats after peripheral nerve lesions. in Balkan Journal of Stomatology. 2004;8(2):109-113.
https://hdl.handle.net/21.15107/rcub_smile_1212 .

Brajović, Gavrilo, "The effects of sorbitol on autotomy in rats after peripheral nerve lesions" in Balkan Journal of Stomatology, 8, no. 2 (2004):109-113,
https://hdl.handle.net/21.15107/rcub_smile_1212 .

TY  - THES
AU  - Brajović, Gavrilo
PY  - 2000
UR  - https://plus.sr.cobiss.net/opac7/bib/1024084878
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/369
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Lokalni neurotropni efekti glicerola i sorbitola na periferni nerv pacova
UR  - https://hdl.handle.net/21.15107/rcub_smile_369
ER  - 

@mastersthesis{
author = "Brajović, Gavrilo",
year = "2000",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Lokalni neurotropni efekti glicerola i sorbitola na periferni nerv pacova",
url = "https://hdl.handle.net/21.15107/rcub_smile_369"
}

Brajović, G.. (2000). Lokalni neurotropni efekti glicerola i sorbitola na periferni nerv pacova. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_smile_369

Brajović G. Lokalni neurotropni efekti glicerola i sorbitola na periferni nerv pacova. 2000;.
https://hdl.handle.net/21.15107/rcub_smile_369 .

Brajović, Gavrilo, "Lokalni neurotropni efekti glicerola i sorbitola na periferni nerv pacova" (2000),
https://hdl.handle.net/21.15107/rcub_smile_369 .