TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Nikolić-Jakoba, Nataša
AU  - Popović, Branka
AU  - Ilić, Vesna
AU  - Mojsilović, Sonja
AU  - Marković, Dragana
AU  - Milošević-Jovčić, Nadežda
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2046
AB  - Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects.
AB  - Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.
PB  - Srpsko lekarsko društvo - Stomatološka sekcija, Beograd
T2  - Stomatološki glasnik Srbije
T1  - Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease
T1  - Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije
VL  - 62
IS  - 2
SP  - 55
EP  - 64
DO  - 10.1515/sdj-2015-0006
ER  - 

@article{
author = "Brajović, Gavrilo and Nikolić-Jakoba, Nataša and Popović, Branka and Ilić, Vesna and Mojsilović, Sonja and Marković, Dragana and Milošević-Jovčić, Nadežda",
year = "2015",
abstract = "Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p lt 0.01) and eight time higher in patients with advanced periodontitis (p lt 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p lt 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects., Uvod Fibronektin može da interreaguje s molekulima imunoglobulina G (IgG) i utiče na normalan klirens ili poremećeno deponovanje imunskih kompleksa. Cilj ovog rada je bio da se ispita veza između fibronektina i IgG različitih glikoformi u gingivalnoj tečnosti osoba obolelih od parodontopatije i parodontalno zdravih ispitanika. Materijal i metode rada U studiju je uključeno 30 pacijenata s umerenom i uznapredovalom parodontopatijom i 22 parodontalno zdrave osobe. U gingivalnoj tečnosti određivan je sadržaj IgG i fibronektina dot blot i imunoblot tehnikama. IgG iz gingivalnih tečnosti su afinitetno izolovani i analizirani na prisustvo fibronektina i ekspresiju galaktoze. Rezultati Sadržaj IgG u gingivalnoj tečnosti osoba s umerenom parodontopatijom bio je oko pet puta veći u odnosu na sadržaj IgG kod zdravih osoba (p lt 0,01), dok je kod uznapredovalih oblika bio oko osam puta veći (p lt 0,001). Takođe, hipogalaktozilovane forme IgG su većoj meri postojale kod osoba sa uznapredovalom parodontopatijom u odnosu na zdrave i osobe s umerenom parodontopatijom (p lt 0,05). U sve tri analizirane grupe dominirali su fibronektinski fragmenti od 48 do 53 kDa. Uočeno je da su IgG izolovani iz gingivalne tečnosti vezani za fragmente fibronektina, pri čemu su IgG osoba sa uznapredovalom parodontopatijom, imali najveću količinu ovih vezanih fragmenata. Zaključak Dobijeni rezultati pokazuju da IgG gingivalne tečnosti zdravih i osoba s parodontopatijom formiraju komplekse sa fibronektinom.",
publisher = "Srpsko lekarsko društvo - Stomatološka sekcija, Beograd",
journal = "Stomatološki glasnik Srbije",
title = "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease, Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije",
volume = "62",
number = "2",
pages = "55-64",
doi = "10.1515/sdj-2015-0006"
}

Brajović, G., Nikolić-Jakoba, N., Popović, B., Ilić, V., Mojsilović, S., Marković, D.,& Milošević-Jovčić, N.. (2015). Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije
Srpsko lekarsko društvo - Stomatološka sekcija, Beograd., 62(2), 55-64.
https://doi.org/10.1515/sdj-2015-0006

Brajović G, Nikolić-Jakoba N, Popović B, Ilić V, Mojsilović S, Marković D, Milošević-Jovčić N. Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease. in Stomatološki glasnik Srbije. 2015;62(2):55-64.
doi:10.1515/sdj-2015-0006 .

Brajović, Gavrilo, Nikolić-Jakoba, Nataša, Popović, Branka, Ilić, Vesna, Mojsilović, Sonja, Marković, Dragana, Milošević-Jovčić, Nadežda, "Interaction between fibronectin fragments and immunoglobulin G in gingival crevicular fluid of patients with periodontal disease" in Stomatološki glasnik Srbije, 62, no. 2 (2015):55-64,
https://doi.org/10.1515/sdj-2015-0006 . .

TY  - JOUR
AU  - Ilić, Vesna
AU  - Milošević-Jovčić, Nadežda
AU  - Marković, D.
AU  - Petrović, S.
AU  - Stefanović, Gordana
PY  - 2007
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1346
AB  - The association between a particular Gm haplotype and susceptibility to multiple myeloma (MM) is not clear. The reason is probably because no investigations have so far been carried out on the relationship between the Gm haplotype, which represents the inherited combination of IgG Gm allotypes, and the Gm allotype expressed at the IgG paraprotein (M-component), which reflects the enhanced gene expression within the haplotype in MM. We studied the incidence of Gm allotypic markers present in IgG subclasses in the serum from 52 patients with MM and in parallel with the isolated IgG paraproteins. The results showed that 84.6% of the patients were heterozygous for haplotypes Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) and 15.3% were homozygous for Gm(f; n/n-; b1; b0; b5), while no homozygous Gm(a; z; n-; g) individuals were found among the studied patients. The incidence of these combinations in the healthy population in Serbia is 34%, 66% and  lt  1%, respectively. Subjects with Gm(a; z; n-; g)/Gm(f; n+/n-; b1; b0; b5) combination are over 10 times [odds ratio (OR) = 10.69; 95% confidence interval 1.67-68] as likely to be affected by the disease as the subjects with homozygous Gm(f; n+/n-; b1; b0; b5) combination (OR = 0.35, 95% confidence interval 0.06-2.23). However, despite the Gm heterozygosity, most of the Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) positive patients with MM (86.3%) had IgG paraprotein with the allotypic marker from the Gm(f; n+/n-; b1; b0; b5) haplotype. Together with patients homozygous for this haplotype, the relative number of patients with serum IgG paraprotein carrying allotypic marker from the Gm(f; n/n-; b1; b0; b5) haplotype was 88.5%. These results suggest that the development of an M-component could be related to a disturbance on chromosome 14q32 carrying the Gm (f; n+/n-; b1; b0; b5) set of genes.
PB  - Wiley, Hoboken
T2  - International Journal of Immunogenetics
T1  - A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development
VL  - 34
IS  - 2
SP  - 119
EP  - 125
DO  - 10.1111/j.1744-313X.2007.00673.x
ER  - 

@article{
author = "Ilić, Vesna and Milošević-Jovčić, Nadežda and Marković, D. and Petrović, S. and Stefanović, Gordana",
year = "2007",
abstract = "The association between a particular Gm haplotype and susceptibility to multiple myeloma (MM) is not clear. The reason is probably because no investigations have so far been carried out on the relationship between the Gm haplotype, which represents the inherited combination of IgG Gm allotypes, and the Gm allotype expressed at the IgG paraprotein (M-component), which reflects the enhanced gene expression within the haplotype in MM. We studied the incidence of Gm allotypic markers present in IgG subclasses in the serum from 52 patients with MM and in parallel with the isolated IgG paraproteins. The results showed that 84.6% of the patients were heterozygous for haplotypes Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) and 15.3% were homozygous for Gm(f; n/n-; b1; b0; b5), while no homozygous Gm(a; z; n-; g) individuals were found among the studied patients. The incidence of these combinations in the healthy population in Serbia is 34%, 66% and  lt  1%, respectively. Subjects with Gm(a; z; n-; g)/Gm(f; n+/n-; b1; b0; b5) combination are over 10 times [odds ratio (OR) = 10.69; 95% confidence interval 1.67-68] as likely to be affected by the disease as the subjects with homozygous Gm(f; n+/n-; b1; b0; b5) combination (OR = 0.35, 95% confidence interval 0.06-2.23). However, despite the Gm heterozygosity, most of the Gm(a; z; n-; g;)/Gm(f; n+/n-; b1; b0; b5) positive patients with MM (86.3%) had IgG paraprotein with the allotypic marker from the Gm(f; n+/n-; b1; b0; b5) haplotype. Together with patients homozygous for this haplotype, the relative number of patients with serum IgG paraprotein carrying allotypic marker from the Gm(f; n/n-; b1; b0; b5) haplotype was 88.5%. These results suggest that the development of an M-component could be related to a disturbance on chromosome 14q32 carrying the Gm (f; n+/n-; b1; b0; b5) set of genes.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Immunogenetics",
title = "A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development",
volume = "34",
number = "2",
pages = "119-125",
doi = "10.1111/j.1744-313X.2007.00673.x"
}

Ilić, V., Milošević-Jovčić, N., Marković, D., Petrović, S.,& Stefanović, G.. (2007). A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development. in International Journal of Immunogenetics
Wiley, Hoboken., 34(2), 119-125.
https://doi.org/10.1111/j.1744-313X.2007.00673.x

Ilić V, Milošević-Jovčić N, Marković D, Petrović S, Stefanović G. A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development. in International Journal of Immunogenetics. 2007;34(2):119-125.
doi:10.1111/j.1744-313X.2007.00673.x .

Ilić, Vesna, Milošević-Jovčić, Nadežda, Marković, D., Petrović, S., Stefanović, Gordana, "A biased Gm haplotype and Gm paraprotein allotype in multiple myeloma suggests a role for the Gm system in myeloma development" in International Journal of Immunogenetics, 34, no. 2 (2007):119-125,
https://doi.org/10.1111/j.1744-313X.2007.00673.x . .

TY  - JOUR
AU  - Stefanović, Gordana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Brajović, Gavrilo
AU  - Petrović, Sonja
AU  - Milošević-Jovčić, Nadežda
PY  - 2006
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1318
AB  - Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.
PB  - Amer Acad Periodontology, Chicago
T2  - Journal of Periodontology
T1  - Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis
VL  - 77
IS  - 11
SP  - 1887
EP  - 1893
DO  - 10.1902/jop.2006.060049
ER  - 

@article{
author = "Stefanović, Gordana and Marković, Dragana and Ilić, Vesna and Brajović, Gavrilo and Petrović, Sonja and Milošević-Jovčić, Nadežda",
year = "2006",
abstract = "Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot-blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) separation of IgG and by capture enzyme-linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA-1) and Bandeiraea simplicifolia (BS-II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypo-galactosylated glycoforms may occur during the process of inflammation of the gingiva.",
publisher = "Amer Acad Periodontology, Chicago",
journal = "Journal of Periodontology",
title = "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis",
volume = "77",
number = "11",
pages = "1887-1893",
doi = "10.1902/jop.2006.060049"
}

Stefanović, G., Marković, D., Ilić, V., Brajović, G., Petrović, S.,& Milošević-Jovčić, N.. (2006). Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology
Amer Acad Periodontology, Chicago., 77(11), 1887-1893.
https://doi.org/10.1902/jop.2006.060049

Stefanović G, Marković D, Ilić V, Brajović G, Petrović S, Milošević-Jovčić N. Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis. in Journal of Periodontology. 2006;77(11):1887-1893.
doi:10.1902/jop.2006.060049 .

Stefanović, Gordana, Marković, Dragana, Ilić, Vesna, Brajović, Gavrilo, Petrović, Sonja, Milošević-Jovčić, Nadežda, "Hypogalactosylation of salivary and gingival fluid immunoglobulin G in patients with advanced periodontitis" in Journal of Periodontology, 77, no. 11 (2006):1887-1893,
https://doi.org/10.1902/jop.2006.060049 . .