TY  - JOUR
AU  - Brajović, Gavrilo
AU  - Popović, Branka
AU  - Puletić, Miljan
AU  - Kostić, Marija
AU  - Milašin, Jelena
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2155
AB  - Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.
AB  - Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Estimation of total bacteria by real-time PCR in patients with periodontal disease
T1  - Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom
VL  - 144
IS  - 1-2
SP  - 10
EP  - 14
DO  - 10.2298/SARH1602010B
ER  - 

@article{
author = "Brajović, Gavrilo and Popović, Branka and Puletić, Miljan and Kostić, Marija and Milašin, Jelena",
year = "2016",
abstract = "Introduction Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55×107) and healthy control (2.37×106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (>7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis., Uvod Parodontopatija se povezuje sa postojanjem povećanog broja bakterija u parodontalnom džepu. Cilj rada Cilj rada je bila kvantifikacija ukupnih bakterija u uzorcima subgingivalnog dentalnog plaka kod osoba obolelih od parodontopatije. Metode rada U 20 uzoraka subgingivalnog plaka ispitanika sa ulceronekroznom parodontopatijom i 10 uzoraka osoba sa zdravim parodoncijumom izvršena je kvantifikacija ukupnog broja bakterija korišćenjem metode qRT- PCR. Kvantifikacija bakterija je zasnovana na određivanju ukupnog broja genskih kopija za rRNK poređenjem sa CT vrednošću standarda. Rezultati Ustanovljena je statistički značajna razlika u prosečnom broju genskih kopija ukupnih bakterija između ispitanika sa parodontopatijom (2,55×107) i ispitanika kontrolne grupe (2,37×106), (p=0,01). Takođe, trend porasta broja genskih kopija ukupnih bakterija s povećanjem dubine parodontalnog džepa (>7 mm) potvrđen je pozitivnom vrednošću koeficijenta korelacije (p=0,073). Zaključak Procena ukupnog broja bakterija na osnovu broja genskih kopija za 16S rRNK može biti važan dodatni parametar u dijagnostikovanju parodontopatije.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Estimation of total bacteria by real-time PCR in patients with periodontal disease, Procena broja ukupnih bakterija primenom real-time PCR metode kod pacijenata sa parodontopatijom",
volume = "144",
number = "1-2",
pages = "10-14",
doi = "10.2298/SARH1602010B"
}

Brajović, G., Popović, B., Puletić, M., Kostić, M.,& Milašin, J.. (2016). Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 144(1-2), 10-14.
https://doi.org/10.2298/SARH1602010B

Brajović G, Popović B, Puletić M, Kostić M, Milašin J. Estimation of total bacteria by real-time PCR in patients with periodontal disease. in Srpski arhiv za celokupno lekarstvo. 2016;144(1-2):10-14.
doi:10.2298/SARH1602010B .

Brajović, Gavrilo, Popović, Branka, Puletić, Miljan, Kostić, Marija, Milašin, Jelena, "Estimation of total bacteria by real-time PCR in patients with periodontal disease" in Srpski arhiv za celokupno lekarstvo, 144, no. 1-2 (2016):10-14,
https://doi.org/10.2298/SARH1602010B . .

TY  - JOUR
AU  - Perunović, Neda
AU  - Rakić, Mia
AU  - Janković, Saša
AU  - Aleksić, Zoran
AU  - Struillou, Xavier
AU  - Čakić, Saša
AU  - Puletić, Miljan
AU  - Leković, Vojislav
AU  - Milašin, Jelena
PY  - 2015
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2058
AB  - The objective of this pilot study was to investigate the potential role of-1562 C>T single nucleotide polymorphism (SNP) in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene as a risk modulator in the development of multiple gingival recessions (MGRs) in young adults in the Serbian population. The study sample comprised 161 systemically healthy people: 60 with MGRs and 101 controls with healthy periodontal tissues. Genotyping was done using polymerase chain reaction/restriction fragment length polymorphism approach on DNA obtained from buccal swabs. Clinical measurements included vertical recession depth (VRD), clinical attachment level (CAL), keratinized gingival width (KGW), visible plaque index (PI), and bleeding on probing (BOP). Heterozygotes (CT) were significantly more frequent in the MGRs group than in the control group (P = .005) and carriers of the T allele had an approximately threefold increase of MGRs risk. Patients with the CT genotype exhibited significantly higher values of VRD and CAL and significantly lower values of KGW than patients with the wildtype genotype. Associations among different genotypes and periodontal biotypes in the MGRs group remained insignificant because all participants exhibited thin biotype. The 1562 C>T SNP in the promoter region of MMP-9 appears to be a risk factor for MGR development and a potential predictor of more severe clinical phenotype.
PB  - Quintessence Publishing Co. Inc.
T2  - International Journal of Periodontics & Restorative Dentistry
T1  - MMP-9-1562 C > T (rs3918242) Promoter Polymorphism as a Susceptibility Factor for Multiple Gingival Recessions
VL  - 35
IS  - 2
SP  - 263
EP  - 269
DO  - 10.11607/prd.2087
ER  - 

@article{
author = "Perunović, Neda and Rakić, Mia and Janković, Saša and Aleksić, Zoran and Struillou, Xavier and Čakić, Saša and Puletić, Miljan and Leković, Vojislav and Milašin, Jelena",
year = "2015",
abstract = "The objective of this pilot study was to investigate the potential role of-1562 C>T single nucleotide polymorphism (SNP) in the promoter region of the matrix metalloproteinase-9 (MMP-9) gene as a risk modulator in the development of multiple gingival recessions (MGRs) in young adults in the Serbian population. The study sample comprised 161 systemically healthy people: 60 with MGRs and 101 controls with healthy periodontal tissues. Genotyping was done using polymerase chain reaction/restriction fragment length polymorphism approach on DNA obtained from buccal swabs. Clinical measurements included vertical recession depth (VRD), clinical attachment level (CAL), keratinized gingival width (KGW), visible plaque index (PI), and bleeding on probing (BOP). Heterozygotes (CT) were significantly more frequent in the MGRs group than in the control group (P = .005) and carriers of the T allele had an approximately threefold increase of MGRs risk. Patients with the CT genotype exhibited significantly higher values of VRD and CAL and significantly lower values of KGW than patients with the wildtype genotype. Associations among different genotypes and periodontal biotypes in the MGRs group remained insignificant because all participants exhibited thin biotype. The 1562 C>T SNP in the promoter region of MMP-9 appears to be a risk factor for MGR development and a potential predictor of more severe clinical phenotype.",
publisher = "Quintessence Publishing Co. Inc.",
journal = "International Journal of Periodontics & Restorative Dentistry",
title = "MMP-9-1562 C > T (rs3918242) Promoter Polymorphism as a Susceptibility Factor for Multiple Gingival Recessions",
volume = "35",
number = "2",
pages = "263-269",
doi = "10.11607/prd.2087"
}

Perunović, N., Rakić, M., Janković, S., Aleksić, Z., Struillou, X., Čakić, S., Puletić, M., Leković, V.,& Milašin, J.. (2015). MMP-9-1562 C > T (rs3918242) Promoter Polymorphism as a Susceptibility Factor for Multiple Gingival Recessions. in International Journal of Periodontics & Restorative Dentistry
Quintessence Publishing Co. Inc.., 35(2), 263-269.
https://doi.org/10.11607/prd.2087

Perunović N, Rakić M, Janković S, Aleksić Z, Struillou X, Čakić S, Puletić M, Leković V, Milašin J. MMP-9-1562 C > T (rs3918242) Promoter Polymorphism as a Susceptibility Factor for Multiple Gingival Recessions. in International Journal of Periodontics & Restorative Dentistry. 2015;35(2):263-269.
doi:10.11607/prd.2087 .

Perunović, Neda, Rakić, Mia, Janković, Saša, Aleksić, Zoran, Struillou, Xavier, Čakić, Saša, Puletić, Miljan, Leković, Vojislav, Milašin, Jelena, "MMP-9-1562 C > T (rs3918242) Promoter Polymorphism as a Susceptibility Factor for Multiple Gingival Recessions" in International Journal of Periodontics & Restorative Dentistry, 35, no. 2 (2015):263-269,
https://doi.org/10.11607/prd.2087 . .

TY  - THES
AU  - Puletić, Miljan
PY  - 2015
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2774
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10939/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47610127
UR  - http://nardus.mpn.gov.rs/123456789/5179
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/980
AB  - Periodontal disease is a chronic inflammatory disease caused by a small group of mainly anaerobic gram-negative bacteria, which leads to the destruction of all tissues of periodontium. Although bacterias are essential they are not enough to lead to the development of disease. Recent data indicate the importance of viruses in the pathogenesis of periodontal disease. The infection leads to inflammatory destruction of periodontal tissues. Oral anaerobic microorganisms and their products, such as lipopolysaccharide (LPS) present in subgingival dental plaque, penetrating into the connective tissue of the gingiva due in systemic circulation through ulcerated and discontinuous epithelium of the periodontal pocket. Currently, it is believed that the pathogenesis of some types of periodontal disease process of multiple steps including the complex interaction between the host, bacteria, viruses, and various environmental factors. The starting hypothesis: for the formation of periodontal bacteria, essential, but not sufficient to lead to the development of the disease, herpes viruses, EBV and CMV may provide answers to some open questions related to the mechanism of degradation of periodontal tissues as well as the consequent complications. Objectives: 1. Identifying presence of specific bacterial flora: Porphyromonas gingivalis(Pg), Tannerella forsythensis(Tf), Prevotella intermedi (Pi) and Aggregatibacter actinomycetemcomitans(A.g). 2. Detection of herpes viridae family members: Epstein Barr virus(EBV), human cytomegalovirus(HCMV) and herpes simplex virus type 1(HSV-1) with complications of periodontal disease (periodontitis and necrotizing ulcer-periodontal abscess)...
AB  - Parodontopatija je hronično inflamatorno oboljenje uzrokovano malom grupom uglavnom anaerobnih Gram-negativnih bakterija, u kom dolazi do destrukcije svih tkiva parodoncijuma. Iako su bakterije esencijalne one nisu dovoljne da dovedu do razvoja bolesti. Noviji podaci ukazuju na značaj virusa u patogenezi parodontopatije. Infekcija dovodi do inflamatorne destrukcije svih tkiva parodoncijuma. Oralni anaerobni mikroorganizmi i njihovi produkti, kao što je lipopolisaharid (LPS) prisutni u subgingivalnom dentalnom plaku, prodorom u vezivno tkivo gingive dospevaju u sistemsku cirkulaciju kroz ulcerisan i diskontinuiran epitel parodontalnog džepa. Trenutno, veruje se da je patogeneza nekih tipova parodontopatije proces od više koraka, uključujući složenu interakciju između domaćina, bakterija, virusa i raznih faktora okoline. Polazna hipoteza : za nastanak parodontopatije bakterije jesu esencijalne ali ne i dovoljne da dovedu do razvoja bolesti, herpes virusi, EBV i CMV mogu dati odgovore na neka otvorena pitanja vezana za mehanizam degradacije parodontalnih tkiva kao i posledičnih komplikacija. Ciljevi istraživanja : 1. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog bakterijskog genoma (Porphyromonas gingivalis (P.g), Tannerella forsythensis (T.f), Prevotella intermedia (P.i) i Aggregatibacter actinomycetemcomitans (A.g) kod komplikacija parodontopatije (ulcero- nekrozna parodontopatija i parodontalni apsces). 2. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog virusnog genoma Epštajn Barov virus (EBV), Humani Citomegalovirus (HCMV) i herpes simplex virus tip 1 (HSV-1) kod komplikacija parodontopatije (ulceronekrozna parodontopatija i parodontalni apsces)...
PB  - Univerzitet u Beogradu, Stomatološki fakultet
T1  - Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease
T1  - Povezanost pojedinih pripadnika familije herpesvirida i parodontopatogenih mikroorganizama sa komplikacijama parodontopatije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5179
ER  - 

@phdthesis{
author = "Puletić, Miljan",
year = "2015",
abstract = "Periodontal disease is a chronic inflammatory disease caused by a small group of mainly anaerobic gram-negative bacteria, which leads to the destruction of all tissues of periodontium. Although bacterias are essential they are not enough to lead to the development of disease. Recent data indicate the importance of viruses in the pathogenesis of periodontal disease. The infection leads to inflammatory destruction of periodontal tissues. Oral anaerobic microorganisms and their products, such as lipopolysaccharide (LPS) present in subgingival dental plaque, penetrating into the connective tissue of the gingiva due in systemic circulation through ulcerated and discontinuous epithelium of the periodontal pocket. Currently, it is believed that the pathogenesis of some types of periodontal disease process of multiple steps including the complex interaction between the host, bacteria, viruses, and various environmental factors. The starting hypothesis: for the formation of periodontal bacteria, essential, but not sufficient to lead to the development of the disease, herpes viruses, EBV and CMV may provide answers to some open questions related to the mechanism of degradation of periodontal tissues as well as the consequent complications. Objectives: 1. Identifying presence of specific bacterial flora: Porphyromonas gingivalis(Pg), Tannerella forsythensis(Tf), Prevotella intermedi (Pi) and Aggregatibacter actinomycetemcomitans(A.g). 2. Detection of herpes viridae family members: Epstein Barr virus(EBV), human cytomegalovirus(HCMV) and herpes simplex virus type 1(HSV-1) with complications of periodontal disease (periodontitis and necrotizing ulcer-periodontal abscess)..., Parodontopatija je hronično inflamatorno oboljenje uzrokovano malom grupom uglavnom anaerobnih Gram-negativnih bakterija, u kom dolazi do destrukcije svih tkiva parodoncijuma. Iako su bakterije esencijalne one nisu dovoljne da dovedu do razvoja bolesti. Noviji podaci ukazuju na značaj virusa u patogenezi parodontopatije. Infekcija dovodi do inflamatorne destrukcije svih tkiva parodoncijuma. Oralni anaerobni mikroorganizmi i njihovi produkti, kao što je lipopolisaharid (LPS) prisutni u subgingivalnom dentalnom plaku, prodorom u vezivno tkivo gingive dospevaju u sistemsku cirkulaciju kroz ulcerisan i diskontinuiran epitel parodontalnog džepa. Trenutno, veruje se da je patogeneza nekih tipova parodontopatije proces od više koraka, uključujući složenu interakciju između domaćina, bakterija, virusa i raznih faktora okoline. Polazna hipoteza : za nastanak parodontopatije bakterije jesu esencijalne ali ne i dovoljne da dovedu do razvoja bolesti, herpes virusi, EBV i CMV mogu dati odgovore na neka otvorena pitanja vezana za mehanizam degradacije parodontalnih tkiva kao i posledičnih komplikacija. Ciljevi istraživanja : 1. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog bakterijskog genoma (Porphyromonas gingivalis (P.g), Tannerella forsythensis (T.f), Prevotella intermedia (P.i) i Aggregatibacter actinomycetemcomitans (A.g) kod komplikacija parodontopatije (ulcero- nekrozna parodontopatija i parodontalni apsces). 2. Ispitati kvantitativno-kvalitativnu zastupljenost specifičnog virusnog genoma Epštajn Barov virus (EBV), Humani Citomegalovirus (HCMV) i herpes simplex virus tip 1 (HSV-1) kod komplikacija parodontopatije (ulceronekrozna parodontopatija i parodontalni apsces)...",
publisher = "Univerzitet u Beogradu, Stomatološki fakultet",
title = "Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease, Povezanost pojedinih pripadnika familije herpesvirida i parodontopatogenih mikroorganizama sa komplikacijama parodontopatije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5179"
}

Puletić, M.. (2015). Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease. 
Univerzitet u Beogradu, Stomatološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_5179

Puletić M. Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_5179 .

Puletić, Miljan, "Relationship between certain members of the family herpesviridae and paropathogenic microorganism with compilcations of periodontal disease" (2015),
https://hdl.handle.net/21.15107/rcub_nardus_5179 .

TY  - JOUR
AU  - Bajić, Miljan
AU  - Janković, Saša
AU  - Milinković, Iva
AU  - Čakić, Saša
AU  - Perunović, Neda
AU  - Novaković, Nada
AU  - Puletić, Miljan
AU  - Aleksić, Zoran
PY  - 2014
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/1918
AB  - Introduction Gingival recession is a displacement of gingival margin apically to cement-enamel junction. Objective The aim of this study was to compare the results achieved with two different surgical procedures used in gingival recession treatment. Methods Ten patients with bilateral buccal recession on maxillary canines or premolars were included in the study. Professional teeth cleaning was performed before surgery. Recession on the experimental side was treated with connective tissue graft in combination with coronally advanced, split thickness flap (tunnel technique). Control side recession was treated with connective tissue graft in combination with trapezoidal coronally advanced, full thickness flap. Coin toss was used for side decision. The following parameters were evaluated before surgery and 6 months post-op: Vertical Recession Dimension, Clinical Attachment Level, Apico-coronal width of the keratinized tissue, Healing index (Laundry), RES index, and Patient evaluation of esthetic results. Student's t-test was used for statistical analysis. Results Six months after surgery, mean root coverage was 91.5±14.1% and 90.1±14.6% on the experimental and on the control side, respectively. RES index, Healing index (Laundry) and Patient Subjective evaluation of esthetic results showed significantly better results (p≤0.05). Conclusion Both surgical procedures produce highly successful clinical results based on evaluated parameters, but this tunnel technique provides significantly better esthetic results.
AB  - Uvod Gingivalna recesija, koja nastaje kao posledica morfoloških nepravilnosti u mukogingivalnom kompleksu, loše utiče na nastanak i prognozu parodontopatije i odlikuje se nezadovoljavajućom estetikom. Cilj rada Cilj ove studije bio je da se uporede rezultati dobijeni primenom dveju različitih hirurških tehnika u lečenju gingivalnih recesija. Metode rada U studiju je uključeno 10 pacijenata Klinike za parodontologiju i oralnu medicinu Stomatološkog fakulteta Univerziteta u Beogradu. Osnovni kriterijum za uključivanje u studiju bilo je postojanje obostranih gingivalnih recesija I ili II klase prema klasifikaciji Milera (Miller) na gornjim očnjacima ili premolarima. Izvođenje studije odobrio je Etički odbor Stomatološkog fakulteta. Ispitanici su podvrgnuti hirurškom lečenju, obostrano, primenom metoda podeljenih usta. Jedna strana tretirana je primenom transplantata vezivnog tkiva tunel- tehnikom (eksperimentalna tehnika), a druga konvencionalnom tehnikom (kontrolna tehnika). Posmatrani klinički parametri - vertikalna dimenzija gingivalne recesije, nivo pripojnog epitela i širina keratinizovane gingive - zabeleženi su pre operacije i šest meseci nakon hirurškog lečenja. Zarastanje u ranoj postoperacionoj fazi procenjivano je na osnovu vrednosti indeksa tkivnog zarastanja. Objektivna procena estetskog ishoda terapije vršena je pomoću indeksa prekrivenosti korena zuba. Rezultati Šest meseci nakon intervencije primenom eksperimentalne tehnike ostvarena je prekrivenost korena od 91,5±14,1%, dok je primenom kontrolne tehnike ostvarena pokrivenost korena od 90,1±14,6%. Parametri za objektivnu procenu postignutih estetskih rezultata i indeksa zarastanja rane pokazali su statistički značajno bolje rezultate ostvarene primenom eksperimentalne tehnike. Zaključak Obe procedure uspešne su u lečenju gingivalne recesije i daju predvidljive rezultate, ali se primenom tunel- tehnike mogu očekivati bolji estetski rezultati.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Utilization of two different surgical techniques in gingival recession treatment: A comparative study
T1  - Komparativna analiza primene dveju tehnika transplantata vezivnog tkiva u lečenju recesija gingive
VL  - 142
IS  - 3-4
SP  - 155
EP  - 163
DO  - 10.2298/SARH1404155B
ER  - 

@article{
author = "Bajić, Miljan and Janković, Saša and Milinković, Iva and Čakić, Saša and Perunović, Neda and Novaković, Nada and Puletić, Miljan and Aleksić, Zoran",
year = "2014",
abstract = "Introduction Gingival recession is a displacement of gingival margin apically to cement-enamel junction. Objective The aim of this study was to compare the results achieved with two different surgical procedures used in gingival recession treatment. Methods Ten patients with bilateral buccal recession on maxillary canines or premolars were included in the study. Professional teeth cleaning was performed before surgery. Recession on the experimental side was treated with connective tissue graft in combination with coronally advanced, split thickness flap (tunnel technique). Control side recession was treated with connective tissue graft in combination with trapezoidal coronally advanced, full thickness flap. Coin toss was used for side decision. The following parameters were evaluated before surgery and 6 months post-op: Vertical Recession Dimension, Clinical Attachment Level, Apico-coronal width of the keratinized tissue, Healing index (Laundry), RES index, and Patient evaluation of esthetic results. Student's t-test was used for statistical analysis. Results Six months after surgery, mean root coverage was 91.5±14.1% and 90.1±14.6% on the experimental and on the control side, respectively. RES index, Healing index (Laundry) and Patient Subjective evaluation of esthetic results showed significantly better results (p≤0.05). Conclusion Both surgical procedures produce highly successful clinical results based on evaluated parameters, but this tunnel technique provides significantly better esthetic results., Uvod Gingivalna recesija, koja nastaje kao posledica morfoloških nepravilnosti u mukogingivalnom kompleksu, loše utiče na nastanak i prognozu parodontopatije i odlikuje se nezadovoljavajućom estetikom. Cilj rada Cilj ove studije bio je da se uporede rezultati dobijeni primenom dveju različitih hirurških tehnika u lečenju gingivalnih recesija. Metode rada U studiju je uključeno 10 pacijenata Klinike za parodontologiju i oralnu medicinu Stomatološkog fakulteta Univerziteta u Beogradu. Osnovni kriterijum za uključivanje u studiju bilo je postojanje obostranih gingivalnih recesija I ili II klase prema klasifikaciji Milera (Miller) na gornjim očnjacima ili premolarima. Izvođenje studije odobrio je Etički odbor Stomatološkog fakulteta. Ispitanici su podvrgnuti hirurškom lečenju, obostrano, primenom metoda podeljenih usta. Jedna strana tretirana je primenom transplantata vezivnog tkiva tunel- tehnikom (eksperimentalna tehnika), a druga konvencionalnom tehnikom (kontrolna tehnika). Posmatrani klinički parametri - vertikalna dimenzija gingivalne recesije, nivo pripojnog epitela i širina keratinizovane gingive - zabeleženi su pre operacije i šest meseci nakon hirurškog lečenja. Zarastanje u ranoj postoperacionoj fazi procenjivano je na osnovu vrednosti indeksa tkivnog zarastanja. Objektivna procena estetskog ishoda terapije vršena je pomoću indeksa prekrivenosti korena zuba. Rezultati Šest meseci nakon intervencije primenom eksperimentalne tehnike ostvarena je prekrivenost korena od 91,5±14,1%, dok je primenom kontrolne tehnike ostvarena pokrivenost korena od 90,1±14,6%. Parametri za objektivnu procenu postignutih estetskih rezultata i indeksa zarastanja rane pokazali su statistički značajno bolje rezultate ostvarene primenom eksperimentalne tehnike. Zaključak Obe procedure uspešne su u lečenju gingivalne recesije i daju predvidljive rezultate, ali se primenom tunel- tehnike mogu očekivati bolji estetski rezultati.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Utilization of two different surgical techniques in gingival recession treatment: A comparative study, Komparativna analiza primene dveju tehnika transplantata vezivnog tkiva u lečenju recesija gingive",
volume = "142",
number = "3-4",
pages = "155-163",
doi = "10.2298/SARH1404155B"
}

Bajić, M., Janković, S., Milinković, I., Čakić, S., Perunović, N., Novaković, N., Puletić, M.,& Aleksić, Z.. (2014). Utilization of two different surgical techniques in gingival recession treatment: A comparative study. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(3-4), 155-163.
https://doi.org/10.2298/SARH1404155B

Bajić M, Janković S, Milinković I, Čakić S, Perunović N, Novaković N, Puletić M, Aleksić Z. Utilization of two different surgical techniques in gingival recession treatment: A comparative study. in Srpski arhiv za celokupno lekarstvo. 2014;142(3-4):155-163.
doi:10.2298/SARH1404155B .

Bajić, Miljan, Janković, Saša, Milinković, Iva, Čakić, Saša, Perunović, Neda, Novaković, Nada, Puletić, Miljan, Aleksić, Zoran, "Utilization of two different surgical techniques in gingival recession treatment: A comparative study" in Srpski arhiv za celokupno lekarstvo, 142, no. 3-4 (2014):155-163,
https://doi.org/10.2298/SARH1404155B . .