TY  - JOUR
AU  - Vasić, Marko
AU  - Loncar-Turukalo, Tatjana
AU  - Tasić, Tatjana
AU  - Matić, Marija
AU  - Glumac, Sofija
AU  - Bajić, Dragana
AU  - Popović, Branka
AU  - Japundžić-Žigon, Nina
PY  - 2019
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2469
AB  - Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Toxicology & Applied Pharmacology
T1  - Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy
VL  - 362
SP  - 43
EP  - 51
DO  - 10.1016/j.taap.2018.10.015
ER  - 

@article{
author = "Vasić, Marko and Loncar-Turukalo, Tatjana and Tasić, Tatjana and Matić, Marija and Glumac, Sofija and Bajić, Dragana and Popović, Branka and Japundžić-Žigon, Nina",
year = "2019",
abstract = "Using comprehensive analysis of heart rate (HRV) and blood pressure (BPV) short-term variability we estimated the time course of changes of autonomic nervous system remodeling in two stages of doxorubicin-induced cardiomyopathy (DCM). We also investigated the level of gene expression of cardiac beta-1 (beta-1AR) and beta-2 (beta-2AR) adrenoceptors. Experiments were performed in adult male Wistar rats equipped with indwelling catheters for BP recording and blood withdrawal. A 15 mg/kg total cumulative dose of doxorubicin was injected i.p. to rats to induce DCM or saline for control (n = 18). Rats were assessed for general toxicity, cardiovascular hemodynamic and echocardiography before treatment (n = 6), 35 days (DOX35; n = 6) and 70 days (DOX70; n = 6) post-treatment. HRV was evaluated by spectral analysis, Poincare plots, sample and approximate entropy. Expression of beta-1AR and beta-2AR mRNA was evaluated by RT-qPCR. Doxorubicin-treated rats exhibited poor general condition and lower survival than saline-treated rats. In DOX35 rats, there were no echocardiography signs of decompensation, no increase in serum cardiac troponins, but there was an increase of HRV and decrease of HR complexity. In these rats typical microscopic signs of cardiotoxicity were seen along with over-expression of beta-1AR mRNA. 70 days post-treatment echocardiography revealed signs of decompensation and serum cardiac troponin T was increased. At this stage BPV decreased. In conclusion, HRV increase matches transient over expression of cardiac beta-1AR mRNA in compensate stage of DCM while decompensate stage of DCM is characterized by a decrease of BPV and no changes in beta-1AR and beta-2AR gene expression.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Toxicology & Applied Pharmacology",
title = "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy",
volume = "362",
pages = "43-51",
doi = "10.1016/j.taap.2018.10.015"
}

Vasić, M., Loncar-Turukalo, T., Tasić, T., Matić, M., Glumac, S., Bajić, D., Popović, B.,& Japundžić-Žigon, N.. (2019). Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology
Academic Press Inc Elsevier Science, San Diego., 362, 43-51.
https://doi.org/10.1016/j.taap.2018.10.015

Vasić M, Loncar-Turukalo T, Tasić T, Matić M, Glumac S, Bajić D, Popović B, Japundžić-Žigon N. Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy. in Toxicology & Applied Pharmacology. 2019;362:43-51.
doi:10.1016/j.taap.2018.10.015 .

Vasić, Marko, Loncar-Turukalo, Tatjana, Tasić, Tatjana, Matić, Marija, Glumac, Sofija, Bajić, Dragana, Popović, Branka, Japundžić-Žigon, Nina, "Cardiovascular variability and beta-ARs gene expression at two stages of doxorubicin - Induced cardiomyopathy" in Toxicology & Applied Pharmacology, 362 (2019):43-51,
https://doi.org/10.1016/j.taap.2018.10.015 . .

TY  - JOUR
AU  - Vasić, N.
AU  - Glumac, Sofija
AU  - Pejić, S.
AU  - Amidžić, L. J.
AU  - Tadić-Latinović, Ljiljana
AU  - Dožić, Branko
AU  - Hinić, S.
AU  - Maksimović, Z.
PY  - 2017
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2226
AB  - Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a complex role in the pathogenesis of atherosclerosis. We compared (1) the histopathological findings in patients with abdominal aortic aneurysms (AAA) and aortoiliac occlusive disease (AOD); (2) the expression of MMP-2/MMP-9 and TIMP-1/TIMP-2 in aortic layers, inflammatory cells and smooth muscle cells (SMCs), aiming to identify the common underlying pathogenic mechanisms of the disease development. Samples were obtained from 30 patients with AAA and 30 with AOD. Aortic histology and immunohistochemistry were performed to evaluate inflammatory changes and MMP and TIMP expression. Thrombosis and ulceration were more frequent in AOD than in AAA. The MMP-9 expression was elevated in all aortic layers of AAA patients and in media/adventitia of AOD patients, mainly followed by lower expression of its inhibitor TIMP-1. Higher MMP-9 expression was also found in SMCs and macrophages of both AAA and AOD specimens, while higher TIMP-1/TIMP-2 were predominantly observed in the lymphocytes and macrophages of the aneurysm. These results showed that both conditions exhibited increased MMP-9 expression; however, the MMP expression pattern differed to some degree between the aneurysms and occlusive disease. The variations in molecular mechanisms underlying dilatative/stenosing disease warrant further investigation.
PB  - Charles Univ Prague, First Faculty Medicine, Prague 6
T2  - Folia Biologica
T1  - Expression of Matrix Metalloproteinases and Endogenous Inhibitors in Abdominal Aortic Aneurysm and Aortoiliac Occlusive Disease (Syndrome Leriche)
VL  - 63
IS  - 5-6
SP  - 209
EP  - 216
UR  - https://hdl.handle.net/21.15107/rcub_vinar_7676
ER  - 

@article{
author = "Vasić, N. and Glumac, Sofija and Pejić, S. and Amidžić, L. J. and Tadić-Latinović, Ljiljana and Dožić, Branko and Hinić, S. and Maksimović, Z.",
year = "2017",
abstract = "Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a complex role in the pathogenesis of atherosclerosis. We compared (1) the histopathological findings in patients with abdominal aortic aneurysms (AAA) and aortoiliac occlusive disease (AOD); (2) the expression of MMP-2/MMP-9 and TIMP-1/TIMP-2 in aortic layers, inflammatory cells and smooth muscle cells (SMCs), aiming to identify the common underlying pathogenic mechanisms of the disease development. Samples were obtained from 30 patients with AAA and 30 with AOD. Aortic histology and immunohistochemistry were performed to evaluate inflammatory changes and MMP and TIMP expression. Thrombosis and ulceration were more frequent in AOD than in AAA. The MMP-9 expression was elevated in all aortic layers of AAA patients and in media/adventitia of AOD patients, mainly followed by lower expression of its inhibitor TIMP-1. Higher MMP-9 expression was also found in SMCs and macrophages of both AAA and AOD specimens, while higher TIMP-1/TIMP-2 were predominantly observed in the lymphocytes and macrophages of the aneurysm. These results showed that both conditions exhibited increased MMP-9 expression; however, the MMP expression pattern differed to some degree between the aneurysms and occlusive disease. The variations in molecular mechanisms underlying dilatative/stenosing disease warrant further investigation.",
publisher = "Charles Univ Prague, First Faculty Medicine, Prague 6",
journal = "Folia Biologica",
title = "Expression of Matrix Metalloproteinases and Endogenous Inhibitors in Abdominal Aortic Aneurysm and Aortoiliac Occlusive Disease (Syndrome Leriche)",
volume = "63",
number = "5-6",
pages = "209-216",
url = "https://hdl.handle.net/21.15107/rcub_vinar_7676"
}

Vasić, N., Glumac, S., Pejić, S., Amidžić, L. J., Tadić-Latinović, L., Dožić, B., Hinić, S.,& Maksimović, Z.. (2017). Expression of Matrix Metalloproteinases and Endogenous Inhibitors in Abdominal Aortic Aneurysm and Aortoiliac Occlusive Disease (Syndrome Leriche). in Folia Biologica
Charles Univ Prague, First Faculty Medicine, Prague 6., 63(5-6), 209-216.
https://hdl.handle.net/21.15107/rcub_vinar_7676

Vasić N, Glumac S, Pejić S, Amidžić LJ, Tadić-Latinović L, Dožić B, Hinić S, Maksimović Z. Expression of Matrix Metalloproteinases and Endogenous Inhibitors in Abdominal Aortic Aneurysm and Aortoiliac Occlusive Disease (Syndrome Leriche). in Folia Biologica. 2017;63(5-6):209-216.
https://hdl.handle.net/21.15107/rcub_vinar_7676 .

Vasić, N., Glumac, Sofija, Pejić, S., Amidžić, L. J., Tadić-Latinović, Ljiljana, Dožić, Branko, Hinić, S., Maksimović, Z., "Expression of Matrix Metalloproteinases and Endogenous Inhibitors in Abdominal Aortic Aneurysm and Aortoiliac Occlusive Disease (Syndrome Leriche)" in Folia Biologica, 63, no. 5-6 (2017):209-216,
https://hdl.handle.net/21.15107/rcub_vinar_7676 .

TY  - JOUR
AU  - Dožić, Branko
AU  - Glumac, Sofija
AU  - Boricić, Novica
AU  - Dozić, Marko
AU  - Aničić, Boban
AU  - Boričić, Ivan
PY  - 2016
UR  - https://smile.stomf.bg.ac.rs/handle/123456789/2141
AB  - Purpose: Adenoid cystic carcinoma (ACC) is one of the most common malignant salivary gland tumors. It is characterized by a high rate of recurrence, perineural invasion and development of distant metastases many years after removal of the primary tumor. Disorders of the induction of apoptosis and its cascade reactions where caspases are involved may be significant in the pathogenesis of this tumor. Methods: The immunohistochemical expression of caspase 9 and caspase 3 was analyzed by tissue microarray (TMA) in 50 cases of ACC in relation with different clinicopathological parameters (gender, age, localization, histological type and overall survival). Results: Caspase 9 was expressed in the cytoplasm and nuclei of ACC tumor cells with varying degrees of staining intensity (1+, 6%; 2+, 54%, 3+, 40%). Comparison of caspase 9 expression in tumor cells with clinicopathological parameters (gender, age, localization, histological type and overall survival) showed no statistically significant difference except that the expression was more pronounced in females. Caspase 3 was expressed in the cytoplasm of tumor cells with varying degrees of staining intensity (1+, 22%; 2+, 36%; 3+, 42%). No correlation between the expression of caspase 3 and clinicopathological parameters was noticed. Conclusions: The expression of caspases 9 and 3 in ACC of the salivary glands can contribute in the better characterization of molecules involved in apoptosis of tumor cells.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Immunohistochemical expression of caspases 9 and 3 in adenoid cystic carcinoma of salivary glands and association with clinicopathological parameters
VL  - 21
IS  - 1
SP  - 152
EP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_smile_2141
ER  - 

@article{
author = "Dožić, Branko and Glumac, Sofija and Boricić, Novica and Dozić, Marko and Aničić, Boban and Boričić, Ivan",
year = "2016",
abstract = "Purpose: Adenoid cystic carcinoma (ACC) is one of the most common malignant salivary gland tumors. It is characterized by a high rate of recurrence, perineural invasion and development of distant metastases many years after removal of the primary tumor. Disorders of the induction of apoptosis and its cascade reactions where caspases are involved may be significant in the pathogenesis of this tumor. Methods: The immunohistochemical expression of caspase 9 and caspase 3 was analyzed by tissue microarray (TMA) in 50 cases of ACC in relation with different clinicopathological parameters (gender, age, localization, histological type and overall survival). Results: Caspase 9 was expressed in the cytoplasm and nuclei of ACC tumor cells with varying degrees of staining intensity (1+, 6%; 2+, 54%, 3+, 40%). Comparison of caspase 9 expression in tumor cells with clinicopathological parameters (gender, age, localization, histological type and overall survival) showed no statistically significant difference except that the expression was more pronounced in females. Caspase 3 was expressed in the cytoplasm of tumor cells with varying degrees of staining intensity (1+, 22%; 2+, 36%; 3+, 42%). No correlation between the expression of caspase 3 and clinicopathological parameters was noticed. Conclusions: The expression of caspases 9 and 3 in ACC of the salivary glands can contribute in the better characterization of molecules involved in apoptosis of tumor cells.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Immunohistochemical expression of caspases 9 and 3 in adenoid cystic carcinoma of salivary glands and association with clinicopathological parameters",
volume = "21",
number = "1",
pages = "152-160",
url = "https://hdl.handle.net/21.15107/rcub_smile_2141"
}

Dožić, B., Glumac, S., Boricić, N., Dozić, M., Aničić, B.,& Boričić, I.. (2016). Immunohistochemical expression of caspases 9 and 3 in adenoid cystic carcinoma of salivary glands and association with clinicopathological parameters. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 21(1), 152-160.
https://hdl.handle.net/21.15107/rcub_smile_2141

Dožić B, Glumac S, Boricić N, Dozić M, Aničić B, Boričić I. Immunohistochemical expression of caspases 9 and 3 in adenoid cystic carcinoma of salivary glands and association with clinicopathological parameters. in Journal of BUON. 2016;21(1):152-160.
https://hdl.handle.net/21.15107/rcub_smile_2141 .

Dožić, Branko, Glumac, Sofija, Boricić, Novica, Dozić, Marko, Aničić, Boban, Boričić, Ivan, "Immunohistochemical expression of caspases 9 and 3 in adenoid cystic carcinoma of salivary glands and association with clinicopathological parameters" in Journal of BUON, 21, no. 1 (2016):152-160,
https://hdl.handle.net/21.15107/rcub_smile_2141 .